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JPS56169582A - Tool for subculture cultivation of microorganism - Google Patents

Tool for subculture cultivation of microorganism

Info

Publication number
JPS56169582A
JPS56169582A JP7377180A JP7377180A JPS56169582A JP S56169582 A JPS56169582 A JP S56169582A JP 7377180 A JP7377180 A JP 7377180A JP 7377180 A JP7377180 A JP 7377180A JP S56169582 A JPS56169582 A JP S56169582A
Authority
JP
Japan
Prior art keywords
container
cultivation
stopping member
microorganism
tool
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP7377180A
Other languages
Japanese (ja)
Other versions
JPS5742307B2 (en
Inventor
Takeo Nomura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Terumo Corp
Original Assignee
Terumo Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Terumo Corp filed Critical Terumo Corp
Priority to JP7377180A priority Critical patent/JPS56169582A/en
Publication of JPS56169582A publication Critical patent/JPS56169582A/en
Publication of JPS5742307B2 publication Critical patent/JPS5742307B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

PURPOSE: To carry out the cultivation and subculture of an anaerobic microorganism by a simple operation, by equipping both a container with its port closed by a hermetic pierceable stopping member and a base plate supported by the stopping member in the container and provided with a medium in its surface.
CONSTITUTION: The port 11a of the container 11 is closed by the hermetic pierceable stopping member 12, e.g., rubber, etc. The base plate 13 forming the medium 14 on its surface is supported by the stopping member 12, the container 11 is decompressed, and the internal atmosphere is converted into an anaerobic atmosphere by carbon dioxide and nitrogen to make a cultivation tool. In using the cultivation tool, the container 11 is communicated with the cultivation bottle 18 storing the culture solution 19 having a microorganism by piercing the stopping member 12 of the container 11 by the needle 17, and the culture solution in an amount corresponding to the degree of reduced pressure of the container 11 is sucked to the container 11, to carry out cultivation.
COPYRIGHT: (C)1981,JPO&Japio
JP7377180A 1980-06-03 1980-06-03 Tool for subculture cultivation of microorganism Granted JPS56169582A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP7377180A JPS56169582A (en) 1980-06-03 1980-06-03 Tool for subculture cultivation of microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP7377180A JPS56169582A (en) 1980-06-03 1980-06-03 Tool for subculture cultivation of microorganism

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP5083582A Division JPS57181686A (en) 1982-03-29 1982-03-29 Device for subculture and cultivation of microorganism

Publications (2)

Publication Number Publication Date
JPS56169582A true JPS56169582A (en) 1981-12-26
JPS5742307B2 JPS5742307B2 (en) 1982-09-08

Family

ID=13527798

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7377180A Granted JPS56169582A (en) 1980-06-03 1980-06-03 Tool for subculture cultivation of microorganism

Country Status (1)

Country Link
JP (1) JPS56169582A (en)

Also Published As

Publication number Publication date
JPS5742307B2 (en) 1982-09-08

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