JPS56140898A - Novel method for determination of number of living bacterial cell - Google Patents
Novel method for determination of number of living bacterial cellInfo
- Publication number
- JPS56140898A JPS56140898A JP4418080A JP4418080A JPS56140898A JP S56140898 A JPS56140898 A JP S56140898A JP 4418080 A JP4418080 A JP 4418080A JP 4418080 A JP4418080 A JP 4418080A JP S56140898 A JPS56140898 A JP S56140898A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- determination
- oxygen
- solution
- membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To determine the number of facterial cells in a solution, easily, rapidly, and accurately, by concentrating the cell solution, and measuring the respiratory activity of the bacterial cells which is proportional to the number of the cells.
CONSTITUTION: A specimen solution which is to be subjected to the determination of the number of bacterial cells, is maintained at 0W5°C for 30W60min, and filtered with a membrane with does not permeate the cell. The membrane 1 is held near the Teflon diaphragm 5 with a holder 2 facing the surface attached with the cells toward the Teflon diaphragm 5 covered with the cathode 4 of the oxygen electrodes. The oxygen electrode is immersed in a phosphoric acid buffer solution containing a nutrient source such as glucose at a specific concentration and saturated with oxygen. About 10min after the application of a definite voltage to the oxygen electrodes, the stationary electric current is measured, and the number of objective living cells can be determined from the level of the current. The method enables the accurate determination of the number of the living cells in a dilute solution containing as low as ≤105/ml of living cells.
COPYRIGHT: (C)1981,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4418080A JPS56140898A (en) | 1980-04-04 | 1980-04-04 | Novel method for determination of number of living bacterial cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4418080A JPS56140898A (en) | 1980-04-04 | 1980-04-04 | Novel method for determination of number of living bacterial cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS56140898A true JPS56140898A (en) | 1981-11-04 |
Family
ID=12684371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4418080A Pending JPS56140898A (en) | 1980-04-04 | 1980-04-04 | Novel method for determination of number of living bacterial cell |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56140898A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6315150A (en) * | 1986-07-08 | 1988-01-22 | Terumo Corp | Method and apparatus for measuring viable cell number |
| EP0435767A2 (en) * | 1989-12-27 | 1991-07-03 | Shimadzu Corporation | Method for determination of aerobic bacteria and apparatus therefor |
| WO1994002632A1 (en) * | 1992-07-22 | 1994-02-03 | Daikin Industries, Ltd. | Method of examining with antibacterial and apparatus therefor |
| WO1994002631A1 (en) * | 1992-07-22 | 1994-02-03 | Daikin Industries, Ltd. | Infectious disease inspection method and apparatus therefor |
| US5654165A (en) * | 1992-07-22 | 1997-08-05 | Daikin Industries, Ltd. | Antibacterial drug inspection method and apparatus therefor |
| US5876959A (en) * | 1992-07-22 | 1999-03-02 | Daikin Industries, Ltd. | Method for testing infectious disease causing microorganisms |
| NL1021258C2 (en) * | 2002-08-12 | 2004-02-17 | Tno | Method and device for determining the number of living cells in a test fluid and the use thereof. |
-
1980
- 1980-04-04 JP JP4418080A patent/JPS56140898A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6315150A (en) * | 1986-07-08 | 1988-01-22 | Terumo Corp | Method and apparatus for measuring viable cell number |
| JPH0545182B2 (en) * | 1986-07-08 | 1993-07-08 | Terumo Corp | |
| EP0435767A2 (en) * | 1989-12-27 | 1991-07-03 | Shimadzu Corporation | Method for determination of aerobic bacteria and apparatus therefor |
| WO1994002632A1 (en) * | 1992-07-22 | 1994-02-03 | Daikin Industries, Ltd. | Method of examining with antibacterial and apparatus therefor |
| WO1994002631A1 (en) * | 1992-07-22 | 1994-02-03 | Daikin Industries, Ltd. | Infectious disease inspection method and apparatus therefor |
| US5654165A (en) * | 1992-07-22 | 1997-08-05 | Daikin Industries, Ltd. | Antibacterial drug inspection method and apparatus therefor |
| US5876959A (en) * | 1992-07-22 | 1999-03-02 | Daikin Industries, Ltd. | Method for testing infectious disease causing microorganisms |
| US6143555A (en) * | 1992-07-22 | 2000-11-07 | Daikin Industries Ltd. | Infectious disease inspection method and apparatus therefor |
| NL1021258C2 (en) * | 2002-08-12 | 2004-02-17 | Tno | Method and device for determining the number of living cells in a test fluid and the use thereof. |
| WO2004015413A1 (en) * | 2002-08-12 | 2004-02-19 | Nederlandse Organisatie Voor Toegepast- Natuurwetenschappelijk Onderzoek Tno | The method and apparatus for determining the number of living cells in a test fluid |
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