JPS5567653A - Measuring method of methotrexate concentration in body fluids and combination of measuring reagent - Google Patents
Measuring method of methotrexate concentration in body fluids and combination of measuring reagentInfo
- Publication number
- JPS5567653A JPS5567653A JP14067878A JP14067878A JPS5567653A JP S5567653 A JPS5567653 A JP S5567653A JP 14067878 A JP14067878 A JP 14067878A JP 14067878 A JP14067878 A JP 14067878A JP S5567653 A JPS5567653 A JP S5567653A
- Authority
- JP
- Japan
- Prior art keywords
- methotrexate
- solution
- reagent
- body fluids
- combination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To enable to measure the methotrexate concentration on body fluids with the pH buffer solution consisting of specific compounds and the reagent consisting of NADPH solution, DHF solution, enzyme solution and methotrexert standard solution, correctly and in a short time.
CONSTITUTION: The reagent kit for the measurement of methotrexate in body fluids is constituted by the combination of (A) the buffer solution of pH 7.5 consisting of 0.1M tris(hydroxymethyl)aminoethane, 0.01M2-mercaptoethanol and KCl 11.2g, (B) NADPH of 0.5W1mM, (C), DHF of 0.5W1mM, (D) engyme having the relative activity 50μ mol/mg/minute or more, and (E) methotrexate standard solution. The measuring time by this reagent is as short as about 10 minutes and the methotrexate in blood and urine can accurately be measured in a short time. Further, without requiring special equipment and location, the measurement can be made by using the spectrophotometer in hospitals and laboratories.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14067878A JPS5567653A (en) | 1978-11-15 | 1978-11-15 | Measuring method of methotrexate concentration in body fluids and combination of measuring reagent |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP14067878A JPS5567653A (en) | 1978-11-15 | 1978-11-15 | Measuring method of methotrexate concentration in body fluids and combination of measuring reagent |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5567653A true JPS5567653A (en) | 1980-05-21 |
JPS5629520B2 JPS5629520B2 (en) | 1981-07-08 |
Family
ID=15274204
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP14067878A Granted JPS5567653A (en) | 1978-11-15 | 1978-11-15 | Measuring method of methotrexate concentration in body fluids and combination of measuring reagent |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5567653A (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5685914A (en) * | 1979-12-14 | 1981-07-13 | Matsushita Electric Ind Co Ltd | Automatic gain control device of television receiver |
-
1978
- 1978-11-15 JP JP14067878A patent/JPS5567653A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPS5629520B2 (en) | 1981-07-08 |
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