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JPS5567653A - Measuring method of methotrexate concentration in body fluids and combination of measuring reagent - Google Patents

Measuring method of methotrexate concentration in body fluids and combination of measuring reagent

Info

Publication number
JPS5567653A
JPS5567653A JP14067878A JP14067878A JPS5567653A JP S5567653 A JPS5567653 A JP S5567653A JP 14067878 A JP14067878 A JP 14067878A JP 14067878 A JP14067878 A JP 14067878A JP S5567653 A JPS5567653 A JP S5567653A
Authority
JP
Japan
Prior art keywords
methotrexate
solution
reagent
body fluids
combination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP14067878A
Other languages
Japanese (ja)
Other versions
JPS5629520B2 (en
Inventor
Atsuyuki Kato
Tadashi Hase
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NIPPON REDARII KK
Pfizer Japan Inc
Original Assignee
NIPPON REDARII KK
Lederle Japan Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NIPPON REDARII KK, Lederle Japan Ltd filed Critical NIPPON REDARII KK
Priority to JP14067878A priority Critical patent/JPS5567653A/en
Publication of JPS5567653A publication Critical patent/JPS5567653A/en
Publication of JPS5629520B2 publication Critical patent/JPS5629520B2/ja
Granted legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To enable to measure the methotrexate concentration on body fluids with the pH buffer solution consisting of specific compounds and the reagent consisting of NADPH solution, DHF solution, enzyme solution and methotrexert standard solution, correctly and in a short time.
CONSTITUTION: The reagent kit for the measurement of methotrexate in body fluids is constituted by the combination of (A) the buffer solution of pH 7.5 consisting of 0.1M tris(hydroxymethyl)aminoethane, 0.01M2-mercaptoethanol and KCl 11.2g, (B) NADPH of 0.5W1mM, (C), DHF of 0.5W1mM, (D) engyme having the relative activity 50μ mol/mg/minute or more, and (E) methotrexate standard solution. The measuring time by this reagent is as short as about 10 minutes and the methotrexate in blood and urine can accurately be measured in a short time. Further, without requiring special equipment and location, the measurement can be made by using the spectrophotometer in hospitals and laboratories.
COPYRIGHT: (C)1980,JPO&Japio
JP14067878A 1978-11-15 1978-11-15 Measuring method of methotrexate concentration in body fluids and combination of measuring reagent Granted JPS5567653A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP14067878A JPS5567653A (en) 1978-11-15 1978-11-15 Measuring method of methotrexate concentration in body fluids and combination of measuring reagent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP14067878A JPS5567653A (en) 1978-11-15 1978-11-15 Measuring method of methotrexate concentration in body fluids and combination of measuring reagent

Publications (2)

Publication Number Publication Date
JPS5567653A true JPS5567653A (en) 1980-05-21
JPS5629520B2 JPS5629520B2 (en) 1981-07-08

Family

ID=15274204

Family Applications (1)

Application Number Title Priority Date Filing Date
JP14067878A Granted JPS5567653A (en) 1978-11-15 1978-11-15 Measuring method of methotrexate concentration in body fluids and combination of measuring reagent

Country Status (1)

Country Link
JP (1) JPS5567653A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5685914A (en) * 1979-12-14 1981-07-13 Matsushita Electric Ind Co Ltd Automatic gain control device of television receiver

Also Published As

Publication number Publication date
JPS5629520B2 (en) 1981-07-08

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