JPS5515004A - High-sensitive and simple coloring calculation method of aceto-acetic acid in blood - Google Patents
High-sensitive and simple coloring calculation method of aceto-acetic acid in bloodInfo
- Publication number
- JPS5515004A JPS5515004A JP8680378A JP8680378A JPS5515004A JP S5515004 A JPS5515004 A JP S5515004A JP 8680378 A JP8680378 A JP 8680378A JP 8680378 A JP8680378 A JP 8680378A JP S5515004 A JPS5515004 A JP S5515004A
- Authority
- JP
- Japan
- Prior art keywords
- aceto
- acetic acid
- blood
- azo compound
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
PURPOSE: To calculate aceto-acetic acid in blood with a high sensitivity simply by making aceto-acetic acid in blood into an azo compound by reaction on p-nitropheny diazonium fluoborate and converting this azo compound to a hydrazo compound and comparing color.
CONSTITUTION: Albuminous substances are eliminated from the blood serum of a tested person, and aceto-acetic acid is made into an azo compound by reaction on p-nitrophenyl diazonium fluoborate in citric acid buffer liquid of pH 4.5. Next, phosphorous acid is added to decompose an excess diazonium salt and convert the azo compound to a hydrazo compound, and this solution has the photo absorptivity measured by a wave length of 390nm, and the measured value is compared with a standard calculation line, thereby calculating aceto-acetic acid in blood. In the figure, the standard calculation line aceto-acetic acid is shown and indicates a clear linearity.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8680378A JPS5844210B2 (en) | 1978-07-18 | 1978-07-18 | Highly sensitive and simple colorimetric determination method for acetoacetic acid in blood |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8680378A JPS5844210B2 (en) | 1978-07-18 | 1978-07-18 | Highly sensitive and simple colorimetric determination method for acetoacetic acid in blood |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5515004A true JPS5515004A (en) | 1980-02-01 |
JPS5844210B2 JPS5844210B2 (en) | 1983-10-01 |
Family
ID=13896949
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8680378A Expired JPS5844210B2 (en) | 1978-07-18 | 1978-07-18 | Highly sensitive and simple colorimetric determination method for acetoacetic acid in blood |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5844210B2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4976522A (en) * | 1988-05-02 | 1990-12-11 | Olympus Optical Co., Ltd. | Objective lens system for endoscopes |
US6618205B2 (en) | 2001-05-14 | 2003-09-09 | Pentax Corporation | Endoscope objective optical system |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6452905U (en) * | 1987-09-30 | 1989-03-31 |
-
1978
- 1978-07-18 JP JP8680378A patent/JPS5844210B2/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4976522A (en) * | 1988-05-02 | 1990-12-11 | Olympus Optical Co., Ltd. | Objective lens system for endoscopes |
US6618205B2 (en) | 2001-05-14 | 2003-09-09 | Pentax Corporation | Endoscope objective optical system |
Also Published As
Publication number | Publication date |
---|---|
JPS5844210B2 (en) | 1983-10-01 |
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