JPH11343247A - Glucosyltransferase inhibitor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a glucosyltransferase inhibitor harmless to both humans and animals, and usable in e.g. preventing, treating dental plaque formation inhibition and periodontosis through its formulation in compositions for oral cavity, foods and feed and the like. SOLUTION: This inhibitor having high glucosyltransferase inhibitory activity is in the form of a solvent extract from at least one kind of plant selected from the group consisting of Parameria laevigate (Juss.) Moldenke (Scientific name) (Indonesian name: Kayu rapat), Eugenia aromatica O.K. (Indonesian name: Cengkeh), Quercus lusitanica Lamk (Indonesian name: Majakani), Illicium verum Hook. f. (Indonesian name: Bunga PK), Eucalyptus alba Reinw. Ex Bl. (Indonesian name: Ceplik sari), Punica granatum L. (Indonesian name: Delima putih), Terminalia belerica Roxb. (Indonesian name: Jakeling), Woodfordia fruticosa (L.) Kurz (Indonesian name: Sidewayah), and Garcinia mangostana L. (Indonesian name: Manggis).

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

FIELD OF THE INVENTION The present invention relates to a glucosyltransferase inhibitor useful as a component of an oral composition for suppressing plaque formation and preventing caries and periodontal disease, and a novel glucosyltransferase useful for food. And a highly safe glucosyltransferase inhibitor.

[0002]

2. Description of the Related Art Caries are generally called caries and are diseases in which teeth are locally and progressively destroyed. As a mechanism of caries, glucosyltransferase derived from oral bacterial streptococci, Streptococcus mutans, and S. sobrinus (hereinafter abbreviated as S. sobrinus) is insoluble and sticky from sucrose in food and drink. Is synthesized, and various bacteria including S. sobrinus proliferate in the synthesized glucan to form plaque as a flora, and then the bacteria in the plaque are produced by the acid produced by sugar fermentation. It is known that dental tissues demineralize and caries progress. Furthermore, it is known that dead bacteria and various substances and enzymes produced by the bacteria have an adverse effect on gums and cause periodontal disease. Therefore, by inhibiting glucosyltransferase, the formation of plaque can be suppressed or prevented, which leads to prevention of dental caries and periodontal disease. In recent years, the prevalence of dental caries and other oral diseases has significantly increased, and various glucosyltransferase inhibitors have been proposed in the course of research on their prevention. For example, JP-A-60-248137 discloses that a compound derived from a microorganism is added to chewing gum or the like, JP-A-59-15231 discloses a condensed tannin, and JP-A-59-152313 discloses a compound. Discloses that Mokuma ephedra and Obayashi faba are useful as anti-cariogenic agents. However, even with such a glucosyltransferase inhibitor, caries and periodontal disease cannot be sufficiently prevented, and no effective glucosyltransferase inhibitor that can be actually used has been found yet. It is.

[0003]

An object of the present invention is to provide a highly safe glucosyltransferase inhibitor which effectively inhibits glucosyltransferase and has no harmful effect on the human body or animals. is there.

[0004]

Means for Solving the Problems The present inventors have conducted intensive studies in order to achieve the above-mentioned object, and have screened various plants to effectively inhibit glucosyltransferase and have high safety from natural sources. When searching for a substance, it was found that an extract from a specific plant had a strong inhibitory effect on glucosyltransferase, and was useful for the prevention of dental caries and periodontal disease. Reached. That is, the present invention provides a scientific name: Parameria laevigata (Juss.) Moldenke (Indonesia name: Kayu rapat), a scientific name: Eugenia aromatica OK (Indonesia name: Ceng)
keh), Scientific name: Quercus lusitanica Lamk (Indonesia name: Ma)
jakani), Scientific name: Illicium verum Hook. f. (Indonesia name: Bu
nga PK), Scientific name: Eucalyptus alba Reinw. ex Bl. (Indonesia name: Ceplik sari), Scientific name: Punica granatum L. (Indonesia name: Delima p)
utih), Scientific name: Terminalia belerica Roxb. (Indonesia name:
Jakeling), Scientific name: Woodfordia fruticosa (L.) Kurz (Indonesia name: Sidowayah), and Scientific name: Garcinia mangostana L. (Indonesia name:
A glucosyltransferase inhibitor comprising, as an active ingredient, a solvent extract of at least one plant selected from the group consisting of Manggis) or an extract extracted by a supercritical extraction method using carbon dioxide. is there. In the present invention, the plant may be a bark of Scientific name: Parameria laevigata (Juss.) Moldenke (Indonesia name: Kayu rapat), Scientific name: Eugenia aromatica OK (Indonesia name: Ceng)
keh) Flower bud, Scientific name: Quercus lusitanica Lamk (Indonesia name: Ma
jakani), Scientific name: Illicium verum Hook. f. (Indonesia name: Bu
nga PK) Fruit, Scientific name: Eucalyptus alba Reinw. ex Bl. (Indonesia name: Ceplik sari) Fruit, Scientific name: Punica granatum L. (Indonesia name: Delima p)
utih peel, scientific name: Terminalia belerica Roxb. (Indonesia name:
Jakeling fruit, scientific name: Woodfordia fruticosa (L.) Kurz (Indonesia name: Sidowayah) flower bud, and scientific name: Garcinia mangostana L. (Indonesia name:
Glucosyltransferase inhibitor, which is at least one member selected from the group consisting of pericarp of Manggis). The present invention is also the glucosyltransferase inhibitor as described above, wherein the extract is a solvent-based extract. The present invention is further the above glucosyltransferase inhibitor, wherein the solvent is at least one selected from the group consisting of chloroform, methanol, ethanol, propylene glycol and 1,3-butylene glycol.

[0005]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail. Ingredients The plant used in the present invention is preferably the scientific name: Bark of Parameria laevigata (Juss.) Moldenke (Indonesia name: Kayu rapat), The scientific name: Eugenia aromatica OK (Indonesia name: Ceng)
keh) Flower bud, Scientific name: Quercus lusitanica Lamk (Indonesia name: Ma
jakani), Scientific name: Illicium verum Hook. f. (Indonesia name: Bu
nga PK) Fruit, Scientific name: Eucalyptus alba Reinw. ex Bl. (Indonesia name: Ceplik sari) Fruit, Scientific name: Punica granatum L. (Indonesia name: Delima p)
utih peel, scientific name: Terminalia belerica Roxb. (Indonesia name:
Fruit of Jakeling, Scientific name: Woodfordia fruticosa (L.) Kurz (Indonesia name: Sidowayah) Flower bud, Scientific name: Garcinia mangostana L. (Indonesia name: Mang)
gis) pericarp. These are usually used after drying. These raw materials are readily available in markets in Indonesia and other countries.

Extraction of Active Ingredients As a method for extracting an active ingredient from the above-mentioned raw materials, known methods can be employed, such as a supercritical extraction method using carbon dioxide gas and an extraction method using a solvent. Before the extraction operation, the raw materials may be divided into appropriate sizes. Examples of the extraction method using a solvent include a method in which a solvent is added to a raw material and heat treatment is performed at a reflux temperature of the solvent. Generally, this heat treatment is preferably carried out at a temperature of 80 ° C. or lower, and an extract can be obtained by performing heat treatment under reflux for 1 to 6 hours using a known extraction device. Alternatively, an extract can be obtained by digesting the dry powder of the raw material in a solvent. The supercritical extraction method and the extraction method using a solvent may be used in combination. These extraction operations are:
It can be repeatedly performed with the raw material residue after the second extraction operation. The amount of solvent used for extraction depends on the amount of raw material 1
100 to 10,000 parts by weight per 100 parts by weight is suitable, and more preferably 300 to 5,000 parts by weight.

The extraction solvent usable in the present invention includes water, lower alcohols such as methanol, ethanol, propyl alcohol, isopropyl alcohol, butanol and isobutanol, and polyhydric alcohols such as propylene glycol and 1,3-butylene glycol. Acetone, dioxane, methyl ethyl ketone, acetonitrile, ethyl acetate, butyl methyl ketone, diethyl ether, dichloromethane, xylene, trichloroethylene, carbon tetrachloride, benzene, chloroform, toluene and the like can be mentioned. Particularly, chloroform, methanol, ethanol, propylene glycol, and 1,3-butylene glycol are preferred. The above solvents may be used alone, or two or more kinds may be used as a mixture, or water and an organic solvent may be used in combination. When the lower alcohol and the polyhydric alcohol are used as hydroalcohols, the water content is preferably 50% or less. The extract thus obtained is concentrated under reduced pressure, and then dried to obtain the extract as a powder. Depending on the solvent used, the extract can be used as it is as an active ingredient of the glucosyltransferase inhibitor. For example, ethanol, propylene glycol, 1,3
-An extract with butylene glycol or the like may be used without removing the solvent.

[0008] Since the supercritical fluid can easily and continuously control its dissolving power by pressure and temperature over a wide range, a supercritical extraction method utilizing this property is expected as a new separation method. . In particular, supercritical carbon dioxide (SC-
Since CO ₂ ) has a relatively low critical point (critical temperature 304.2 K, critical pressure 72.8 atm), it can be applied to thermally unstable substances. Supercritical extraction is used in food, chemistry,
This is an extraction technology that has attracted attention in a wide range of fields, such as the pharmaceutical and cosmetic industries. In particular, when carbon dioxide is used as an extractant, its critical point (75 kg / cm ² , 31 ° C.) is relatively low and the safety is high, so that components which are unstable to heat and components with high volatility are used. Can be efficiently extracted and separated. Extraction of a target component from a raw material can be achieved by setting the pressure and temperature of carbon dioxide at or above a critical point. Generally, at the same temperature, the higher the pressure, the higher the dissolving power of supercritical carbon dioxide. The supercritical carbon dioxide extraction in the present invention can be performed by a method known per se. For example, using a supercritical extraction device, the temperature of the high-pressure cell section is 33 to 40 ° C., preferably 35 ° C., and the pressure is 75 to 300 a.
tm, preferably a 150 atm, carbon dioxide flow rate 0.5~5.0dm ³ / min, preferably 4.0dm ³ /
Minutes. Thereby, the extract containing the glucosyltransferase inhibitor can be efficiently collected.

The glucosyltransferase inhibitor of the present invention may be appropriately added to the glucosyltransferase inhibitor of the present invention, in addition to the extract of the above-mentioned raw material, which is an active ingredient, as long as the extract is not harmful to the extract and unsuitable for products utilizing the glucosyltransferase inhibitor. The agent can be blended according to a conventional method. Also, glycyrrhizic acid, dipotassium glycyrrhizinate, lysozyme chloride, lytic enzyme, mutanase, chlorhexidine,
Sorbic acid, alexidine, hinokitiol, cetylpyridinium chloride, alkyl glycine, alkyl diaminoethyl glycine salt, allantoin, ε-aminocaproic acid, azulene, vitamin E and derivatives thereof, sodium monofluorophosphate, sodium fluoride, stannous fluoride Other active ingredients such as water-soluble primary or secondary phosphates, quaternary ammonium compounds, and sodium chloride can also be blended.

Application to Products The glucosyltransferase inhibitor of the present invention can be added to oral compositions, foods, feeds, etc. according to a conventional method, and the amount added can be appropriately selected according to the type of product. Usually, the amount of the extract is preferably 0.001 to 5% by weight relative to the total weight of the product, and more preferably 0.005 to 2% by weight. As a product to which the glucosyltransferase inhibitor of the present invention is applied, specifically, dentifrices such as toothpaste, powdered toothpaste, water dentifrice, mouthwash, troche, gargle tablets, oral compositions such as coating agents, pasta, Foods such as chewing gum, chewing jelly and coffee beverages, and feeds such as dog food and cat food are included.

[0011] In addition to the glucosyltransferase inhibitor of the present invention, these products may be appropriately blended with additives depending on various products, and other active ingredients may be used. Of course, these additives may be blended with the glucosyltransferase inhibitor itself of the present invention. For example, in the case of toothpaste, abrasives such as hydroxyapatite, monobasic calcium phosphate, dibasic calcium phosphate (dihydrate and anhydrous), tribasic calcium phosphate, and calcium carbonate (either light or heavy, Quality is preferred), calcium pyrophosphate, insoluble sodium metaphosphate, tribasic magnesium phosphate, calcium sulfate,
Amorphous silica, crystalline silica, aluminosilicate, zirconosilicate, aluminum oxide, aluminum hydroxide, resin, etc .; as a binder, carboxymethylcellulose, hydroxyethylcellulose, alginate, carrageenan, gum arabic, polyvinyl alcohol, etc .; As an agent, polyethylene glycol, sorbitol,
Glycerin, propylene glycol, etc .;
Sodium lauryl sulfate, sodium dodecylbenzenesulfonate, hydrogenated coconut fatty acid monoglyceride monosulfate sodium, sodium lauryl sulfoacetate,
Sodium N-lauroyl sarcosinate, N-acyl glutamate, sucrose fatty acid ester and the like; essential oils such as peppermint and spearmint as flavoring agents, 1-menthol,
Carvone, eugenol, anethole and the like; sweeteners include sodium saccharin, stevioside, neohesperidyl dihydrochalcone, glycyrrhizin, perillartin, p-methoxycinnamic aldehyde and the like; and preservatives. These components and the glucosyltransferase inhibitor of the present invention or an extract which is an active ingredient of the inhibitor are mixed with water, and the mixture is produced according to a conventional method. Also, in mouthwashes such as mouthwashes and the like, components according to the properties of the product are appropriately compounded.

[0012]

Industrial Applicability The glucosyltransferase inhibitor of the present invention has no harmful effects on humans and animals, and is extremely safe. Therefore, by incorporating the present glucosyltransferase inhibitor into oral compositions, foods, feeds, etc., the formation of plaque by S. sobrinus-derived glucosyltransferase is effectively suppressed, and thus the occurrence of dental caries and periodontal disease are prevented. It can be prevented very effectively.

Hereinafter, production examples, test examples and application examples will be given.
The present invention will be described in more detail, but the present invention is not limited thereto.

Production Example 1 Scientific name: 100 g of dried bark of Parameria laevigata (Juss.) Moldenke (Indonesia name: Kayu rapat)
Then, 1,000 ml of 50 v / v% ethanol was added thereto, and the mixture was extracted with stirring at room temperature in a dark place for 7 days. This was centrifuged and filtered under pressure to obtain an extract. This extract was freeze-dried to obtain an extract. Production Example 2 Scientific name: Eugenia aromatica OK (Indonesia name: Ceng
keh) 50 g for 100 g of crushed dried flower buds
/ v% ethanol (1,000 ml)
Stirring extraction was performed for a day. This is centrifuged and filtered under pressure,
An extract was obtained. This extract was freeze-dried to obtain an extract. Production Example 3 Scientific name: Quercus lusitanica Lamk (Indonesia name: Ma
jakani) 5
1,000 ml of 0 v / v% ethanol was added, and the mixture was stirred and extracted in a dark place at room temperature for 7 days. This was centrifuged and filtered under pressure to obtain an extract. This extract was freeze-dried to obtain an extract. Production Example 4 Scientific name: Illicium verum Hook. F. (Indonesia name: Bu
nga PK) 50 g for 100 g of crushed real dried product
/ v% ethanol (1,000 ml)
Stirring extraction was performed for a day. This is centrifuged and filtered under pressure,
An extract was obtained. This extract was freeze-dried to obtain an extract. Production Example 5 Scientific name: 100 g of crushed dried fruit pulp of Eucalyptus alba Reinw. Ex Bl. (Indonesia name: Ceplik sari)
Then, 1,000 ml of 50 v / v% ethanol was added thereto, and the mixture was extracted with stirring at room temperature in a dark place for 7 days. This was centrifuged and filtered under pressure to obtain an extract. This extract was freeze-dried to obtain an extract. Production Example 6 Scientific name: Punica granatum L. (Indonesia name: Delima p
utih) per 100 g of crushed dried pericarp
/ v% ethanol (1,000 ml)
Stirring extraction was performed for a day. This is centrifuged and filtered under pressure,
An extract was obtained. This extract was freeze-dried to obtain an extract. Production Example 7 Scientific name: Terminalia belerica Roxb. (Indonesia name:
Jakeling) 50 g per 100 g of crushed real dried product
1,000 ml of v / v% ethanol was added, and the mixture was stirred and extracted in a dark place at room temperature for 7 days. This was centrifuged and filtered under pressure to obtain an extract. This extract was freeze-dried to obtain an extract. Production Example 8 Scientific name: Woodfordia fruticosa (L.) Kurz (Indonesia name: Sidowayah) To 100 g of dried florets crushed product, 1,000 ml of 50 v / v% ethanol was added, and the mixture was stirred at room temperature in a dark place for 7 days. An extraction was performed. This was centrifuged and filtered under pressure to obtain an extract. This extract was freeze-dried to obtain an extract. Production Example 9 Scientific name: Garcinia mangostana L. (Indonesia name: Mang
gis) per 100 g of crushed dried pericarp
/ v% ethanol (1,000 ml)
Stirring extraction was performed for a day. This is centrifuged and filtered under pressure,
An extract was obtained. This extract was freeze-dried to obtain an extract. Production Example 10 Scientific name: Garcinia mangostana L. (Indonesia name: Mang
gis) was charged into an extraction tower using 100 g of the crushed dried pericarp as a raw material, and extraction was performed under the conditions of a carbon dioxide pressure of 250 kg / cm ² and a temperature of 52 ° C., and thereafter, separation of carbon dioxide and the extract was performed. Was. The pressure of carbon dioxide was reduced to atmospheric pressure, and the obtained oil was used as an extract.

Test Examples Assay of Glucosyltransferase Inhibitory Activity The extracts obtained in the above Production Examples 1 to 9 were evaluated by the following method. i) Preparation of glucosyltransferase S. sobrinus 6715 strain was isolated from Todd-Hewitt broth (Difco)
The cells were cultured in a medium for 24 hours at 37 ° C., and the cells were extracted with 8 M urea at room temperature for 1 hour. The extract was dialyzed against 10 mM phosphate buffer (pH 6.0) to obtain a glucosyltransferase enzyme standard solution. ii) Measurement of glucosyltransferase inhibitory activity 100 mM phosphate buffer (pH 6.0), 1% sucrose,
1 mg / ml Dextran T 10, 10 mg of extract of Production Examples 1-9
A sample solution prepared at 0, 50, 15, and 5 μg / ml and a glucosyltransferase enzyme standard solution were mixed, and subjected to an enzyme reaction in a glass test tube at 37 ° C. for 3 hours. At this time, the amount of the enzyme was adjusted to an absorbance at 550 nm of 1.0 at 37 ° C. for 3 hours.
Was used in this experiment. The insoluble glucan was sonicated and the absorbance at 550 nm was measured as follows. Absorbance before the reaction using the sample solution (A), absorbance after the reaction using the sample solution (B), absorbance before (C) and after (D) using water instead of the sample solution The inhibition rate (%) was determined by the following formula. Glucosyltransferase inhibition rate (%) = [1- (BA) / (DC)] × 1
Iii) Measurement Results The results of measuring the glucosyltransferase inhibitory activity when using the sample solutions prepared with the extracts of Production Examples 1 to 9 using the above reaction system are shown in Table 1 below. It was clarified that the sample showed a higher inhibition rate at a lower sample concentration than the green tea extract or oolong tea extract used as a control.

[0016]

[Table 1] 検 Test sample inhibition rate (%) ──試 料 Sample concentration (μg / ml) 100 50 15 5 ──────────────────────────── ──────── Scientific name: 95.3 94.8 71.4 25.8 Bark extract of Parameria laevigata (Juss.) Moldenke Scientific name: Eugenia aromatica OK flower bud extract 94 93.2 86 6.1 Scientific name: Quercus lusitanica Lamk insect extract 96.9 93.5 93.3 90.3 Scientific name: Real extract of Illicium verum Hook. F. 95.8 92 83.4 7 Scientific name: 95.3 93 of Eucalyptus alba Reinw. Ex Bl. 91.9 32.6 Real extract Scientific name: Pericarp extract of Punica granatum L. 97 95.9 93.9 85.7 Scientific name : Real extract of Terminalia belerica Roxb. 97.4 94.7 93.5 83.2 Scientific name: Woodfordia fruticosa (L.) Kurz 96.8 97.6 98.5 96.9 Flower bud extract Scientific name: Pericarp extract of Garcinia mangostana L. 84.1 95.5 92.5 46.9 Green tea extract 40.0 22.0 3.4 1.1 Oolong tea extract 82.0 50.0 31.1 4.5 ────────────────────────────────────

Application Example 1 Toothpaste was manufactured according to the following Table 2 according to a conventional method. In addition, in the following application examples,% represents weight%.

[0018]

[Table 2] ─────────────────────────────── Component amount (%) ───────── ────────────────────── Dicalcium phosphate dihydrate 45.0 Sodium carboxymethyl cellulose 1.0 Sorbitol 10.0 Glycerin 10.0 Sodium lauryl sulfate 1.5 Fragrance 1.0 Saccharin sodium 0.15 Extract obtained in Production Example 1 0.1 Purified water qs合計 Total 100.0 ───────────────────────────────

Application Example 2 A gargle was prepared according to the recipe shown in Table 3 below according to a conventional method.

[0020]

[Table 3] ─────────────────────────────── Component amount (%) ───────── ────────────────────── Ethanol 20.0 Saccharin sodium 0.2 Fragrance 1.00 Glycerin 5.0 Chlorhexidine 0.005 Extract extract obtained in Production Example 2 1.0 Appropriate amount of purified water ─────────────────────────────── Total 100.0 ───────── ──────────────────────

Application Example 3 A gingival massage cream was produced according to the recipe shown in Table 4 below according to a conventional method.

[Table 4] ─────────────────────────────── Component Content (%) ─────────白色 White petrolatum 25.0 Stearyl alcohol 22.0 Propylene glycol 12.0 Sucrose stearic acid ester 0.6 Ethyl parahydroxybenzoate 0 .025 Propyl parahydroxybenzoate 0.015 Extract obtained in Production Example 3 0.1 Appropriate amount of purified water ───────────────────────────合計 Total 100.0 ───────────────────────────────

Application Example 4 Chewing jelly was produced according to the composition and procedure shown in Table 5 below.

[0023]

[Table 5] ──────────────────── Granulated sugar 31.0 starch syrup 29.0 gelatin 7.7 sorbitol 4.2 citric acid 0.7 Extract obtained in Production Example 4 0.05 Water (A) 11.8 Water (B) 15.5 Colorant 0.001 ────────────────────────────合計 Total 100.0 ─────────────────────────────────

After the granulated sugar, starch syrup and sorbitol are boiled down in water (A) to 130 ° C. (sugar content 85 ° C.),
Cooled to 15 ° C. Further, gelatin swelled with water (B) was added and dissolved, and citric acid, a methanol extract, a coloring agent, and a flavoring agent were combined, molded and cooled (25 ° C., 18 hours).

Application Example 5 According to a conventional method, a coffee beverage was produced according to the formulation shown in Table 6 below.

[0026]

[Table 6] ───────────────────────────────── Component amount (%) ─────── ────────────────────────── Instant coffee 1.7 Granulated sugar 5.0 Extract obtained in Production Example 5 0.05 Water (or Yu) Suitable amount ───────────────────────────────── Total 100.0 ────────── ───────────────────────

Application Example 6 A chewing gum was produced according to a conventional method according to the formulation shown in Table 7 below.

[0028]

[Table 7] ─────────────────────────────────── Component amount (%) ───── ────────────────────────────── Gum base 31.6 Granulated sugar 62.5 Glycerin 0.8 Citric acid 1.0 Sucrose Palmitate 1.0 Tricalcium phosphate 2.0 Ethanol extract obtained in Production Example 6 0.1 Fragrance 1.0合計 Total 100.0 ────────────────────────────────── ─

Application Example 7 According to a conventional method, dog food was produced according to the formulation and procedure shown in Table 8 below.

[0030]

[Table 8] ───────────────────────────────── Component amount (%) ─────── ────────────────────────── Flour 30.0 Cornflower 15.0 Soy flour 15.0 Meatmeal 20.0 Sugar 5.0 Tallow 5.0 Salt 1.0 Calcium phosphate 1.5 Potassium sorbate 0.3 Perfume 0.6 Extract obtained in Production Example 7 0.1 Propylene glycol 6.5合計 Total 100.0 ────────────────────────── ───────

40 parts by weight of water was added to 100 parts by weight of the above compound, and the mixture was extruded by an extruder at 150 ° C. and a screw compression ratio of 1: 3.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 7/26 A23L 2/00 F

Claims (4)

[Claims] [Claim 1] Scientific name: Parameria laevigata (Juss.) Mo
ldenke (Indonesia name: Kayu rapat), Scientific name: Eugenia aromatica OK (Indonesia name: Ceng)
keh), Scientific name: Quercus lusitanica Lamk (Indonesia name: Ma)
jakani), Scientific name: Illicium verum Hook. f. (Indonesia name: Bu
nga PK), Scientific name: Eucalyptus alba Reinw. ex Bl. (Indonesia name: Ceplik sari), Scientific name: Punica granatum L. (Indonesia name: Delima p)
utih), Scientific name: Terminalia belerica Roxb. (Indonesia name:
Jakeling), Scientific name: Woodfordia fruticosa (L.) Kurz (Indonesia name: Sidowayah), and Scientific name: Garcinia mangostana L. (Indonesia name:
A glucosyltransferase inhibitor comprising, as an active ingredient, at least one plant extract selected from the group consisting of Manggis) or an extract extracted by a supercritical extraction method using carbon dioxide. 2. The plant is scientifically named: Parameria laevigata (J
uss.) Bark of Moldenke (Indonesia name: Kayu rapat), Scientific name: Eugenia aromatica OK (Indonesia name: Ceng)
keh) Flower bud, Scientific name: Quercus lusitanica Lamk (Indonesia name: Ma
jakani), Scientific name: Illicium verum Hook. f. (Indonesia name: Bu
nga PK) Fruit, Scientific name: Eucalyptus alba Reinw. ex Bl. (Indonesia name: Ceplik sari) Fruit, Scientific name: Punica granatum L. (Indonesia name: Delima p)
utih peel, scientific name: Terminalia belerica Roxb. (Indonesia name:
Jakeling fruit, scientific name: Woodfordia fruticosa (L.) Kurz (Indonesia name: Sidowayah) flower bud, and scientific name: Garcinia mangostana L. (Indonesia name:
2. The glucosyltransferase inhibitor according to claim 1, which is at least one member selected from the group consisting of pericarp of Manggis). 3. The glucosyltransferase inhibitor according to claim 1, which comprises a solvent extract of a plant as an active ingredient. 4. The method according to claim 1, wherein the solvent is water, chloroform, methanol,
The glucosyltransferase inhibitor according to any one of claims 1 to 3, which is at least one member selected from the group consisting of ethanol, propylene glycol, and 1,3-butylene glycol.