JPH11311615A - Instrument for measuring substance in urine or urine ph - Google Patents

Abstract

PROBLEM TO BE SOLVED: To enable measuring equipment to be easily applied, without being affected by an interference substance in urine by forming an organic substance film that can be converted into a substance that is capable of reacting with a substance to be measured in urine at the insulation ion-sensitive part of an ion sensitive field effect transistor(ISFET), and by providing a structure for storing urine around the organic substance film. SOLUTION: In a dunted portion 3 being formed by an ISFET substrate 1 and an armor part 2, an insulation ion-sensitive part 4 of an ISFET is formed, and an organic substance film 5 is fixed onto the insulation ion-sensitive part 4 by an adhesive 6 for sealing. The insulation ion-sensitive part 4 of the ISFET, namely the interface potential of the surface of a gate surface, has a pH dependence property, and the interface potential is measured for measuring the pH of measurement sample solution. With measuring glucose instruct or pH measuring instruct in urine using the ISFET, an interface potential measurement circuit, a CPU, and the like are formed into a single body, the measuring instrument being subjected to waterproofing is installed at an arbitrary position in a toilet, and measurement constants are sent to an indicator by radio.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a urine substance measuring device or urine pH measuring device. More specifically, the present invention relates to a urine substance measuring instrument or urine pH measuring instrument which enables measurement without being affected by interfering substances in urine.

[0002]

2. Description of the Related Art In a conventional urine measurement system, for example, a certain amount of urine is collected and the state of urine or the concentration of a substance in the urine is determined based on the coloration using a test paper or the like, or urine is collected. Using a system of a complicated configuration composed of a vessel, a urine washer, a pump, a pipe for reagent supply liquid, a pipe for urine transport, a current detection type electrochemical biosensor measuring instrument or an optical measuring instrument and a display, It is common practice to measure the concentration of a substance in urine.

When the former test paper is used, the measurement is not economical because it lacks quantitativeness and is disposable. Also, in the latter urine measurement system, a current detection type electrochemical biosensor is used as a measuring device, and accurate measurement cannot be performed due to the influence of interfering substances in urine, such as ascorbic acid. There is a disadvantage that.
That is, in the case of a current detection type electrochemical biosensor, ascorbic acid or the like is oxidized on the electrode to apply a potential to the electrode, and the current value cannot avoid an error in the measurement.

In addition to the above, when an optical measuring device is used, not only a special reagent for colorimetry is required, but also a separate washing device is required to prevent urine from remaining on the measuring device after the measurement. In need of. Furthermore, since the urine collector is fixed or semi-fixed, it cannot be easily applied to users, for example, men, women and children. Moreover,
Auxiliary equipment such as reagents, buffers, washing solutions, pumps, urine collectors, and the like require complicated maintenance, for example, replacement of reagents. Also, since the system itself is large-scale as in the case described above, there is a problem in economy.

[0005]

SUMMARY OF THE INVENTION An object of the present invention is to provide a urine substance measuring device or urine pH measuring device which is not affected by interfering substances in urine and which does not require extra auxiliary equipment. To provide.

[0006]

SUMMARY OF THE INVENTION The object of the present invention is as follows.
An organic material film that can convert the substance to be measured in urine into a material that can respond to the insulating ion-sensitive part was formed on the insulating ion-sensitive part of the ion-sensitive field-effect transistor, and a structure was provided around which urine accumulated. This is achieved by a urine substance measuring device or a urine pH measuring device having a structure in which an organic substance film is removed from such a configuration.

Here, an organic substance film capable of converting a substance to be measured in urine into a substance capable of responding to the insulating ion sensitive part is, for example, glucose capable of converting glucose into gluconic acid capable of reacting with the insulating ion sensitive part. Like an oxidase membrane,
It refers to oxidoreductase film, antibody film, complex film and the like.

[0008]

DETAILED DESCRIPTION OF THE INVENTION Ion-sensitive field effect transistors (ISFETs) are known as pH sensors made from semiconductor materials. The interface potential of the insulating ion sensitive part of the ISFET, that is, the gate surface has a pH dependency, and the pH of the measurement sample solution can be measured by measuring the interface potential. ISFET is a potential detection type sensor, so unlike the current detection type electrochemical sensor used as a base sensor of ordinary biosensors, it has the advantage that it is not affected by ascorbic acid, which is an interfering substance contained in urine .

When the amount of glucose is to be measured using such an ISFET, glucose oxidase (GOD) is immobilized on an insulating ion-sensitive portion (gate) of the ISFET. The formation of the immobilized membrane of GOD on the ISFET is performed, for example, by preparing an aqueous solution containing a final concentration of 1% by weight of GOD (180,000 U / g), 0.2% by weight of glutaraldehyde, and 1% by weight of bovine serum albumin. This is performed by dropping 1 μl of an aqueous solution onto the vicinity of the gate of the ISFET and drying at 40 ° C. for 30 minutes.

The principle of measuring the glucose amount of the GOD-immobilized ISFET is as follows. Since the generated gluconic acid is acidic, the ISFET below the GOD-immobilized membrane detects gluconic acid generation. At that time, ISFET
Since the change in the interfacial potential measured in step (1) is proportional to the glucose concentration, the glucose concentration in urine can be measured.

FIG. 1 is a plan view of one embodiment of a urine substance measuring instrument.
(a) and the center line cross section (b). ISFET
The recess 3 formed by the base 1 and the exterior parts 2 and 2 has an ISFET
Is formed, and an organic material film 5 is fixed thereon with an adhesive 6 and sealed.

[0012] In the case of a urine pH meter, the pH is measured by ISFET itself.
Therefore, an organic material film that can be converted into a material sensitive to the insulating ion sensitive part is not required.

One embodiment of a urine pH measuring device is shown in FIG.
And a center line sectional view (b). In this case, since an organic material film is not required, the insulating ion sensitive part 4 of the ISFET is directly exposed to the depression 3 formed by the ISFET base 1 and the exterior parts 2. The adhesives 6, 6 seal the ends of the exterior parts 2, 2.

The sensitive part of the ISFET is exposed on the surface so as to come into contact with urine indirectly or directly through an organic material film. In this case, urine is indirectly indirectly connected to the sensitive part of the ISFET which is a sensor. Alternatively, it is preferable to have a structure in which urine accumulates around the sensor so as to make direct contact. Here, the structure in which urine accumulates is arbitrary, and may be, for example, a depression having a sensitive portion at the bottom. Therefore, a special device such as a urine collector is not required.

The urine substance measuring instrument and the urine pH measuring instrument can be provided on the same exterior substrate, and the two can be measured simultaneously.

A urine glucose meter or pH meter manufactured by using ISFET is a device in which an interfacial potential measuring circuit, an electromagnetic wave transmitter, a battery and a CPU are integrated, and the waterproofed measuring device is on the water surface. Attached at any location in the toilet that urine can contact. The attachment is performed by an arbitrary method such as a method using a suction cup, an adhesive, a double-sided tape, a magnet, or the like, or a method of directly processing a toilet and applying means such as standing, screwing, hooking, and the like.

The mounting position is arbitrary as long as it is a position in the toilet bowl to which urine can come into contact, as described above. In particular, it is the surface where urine is released and comes into contact, and the residual urine in the measuring section after measurement is measured. Is preferably a position to be washed. In addition, since the washing is automatically performed by washing with water, there is an advantage that residual urine, which is a measurement error, does not remain.

The contents measured by such a measuring device are sent to the display device wirelessly. The display is an electromagnetic wave receiver, CP
It is composed of a U, a display unit, a battery or a 100V power supply, and is attached to a toilet wall or the like. When urine is released, some of which come into indirect or direct contact with the ISFET sensitive part of the meter,
The measuring device measures the amount of glucose and pH in urine, and outputs the corresponding output to an indicator using electromagnetic waves (γ-ray, X-ray, ultraviolet, visible light, near infrared, infrared, far infrared, microwave, radio wave, etc.). send.

The display receives this electromagnetic wave and displays a state or a measured value according to the amount of glucose or pH. Since the concentration composition is set in the measuring instrument in advance, for example, the measured value is higher than the standard value, so be careful about the contents of the meal.Glucose concentration is 100 ± 20 mg / dl State display and measured value Display is enabled.

In such measurement and display, there is no need for a calibration reagent or a buffer solution during the measurement, and additional equipment such as piping and pumps for the measurement and is not an optical detector. No special reagents are required.

Urine collected in the ISFET sensitive part of the measuring instrument is replaced with water by a normal washing operation with water, and is prepared for the next measurement. The start and end of measurement are performed by starting the measuring device by operating the buttons on the display by an operator, automatically starting the measuring device and the display by infrared rays, etc. It is also possible to start up by a person who operates only the display.

The substance immobilized on the ISFET of the measuring instrument includes various enzymes other than GOD, antibodies, complexes and the like.
By the action of these substances, proteins, uric acid, occult blood,
Pregnancy can be measured.

[0023]

According to the present invention, the urine substance measuring instrument or urine
The pH meter is not affected by interfering substances in urine,
It can be easily applied to men, women, children, etc., and hardly requires the auxiliary equipment that was conventionally required,
It is also excellent in terms of economics, and this is a home health check
(Self-care), can be used effectively for community health examinations, etc.

[Brief description of the drawings]

FIG. 1 is a plan view of one embodiment of a urine substance measuring instrument according to the present invention.
(a) and a center line sectional view (b).

FIG. 2 is a plan view of one embodiment of a urine pH measuring device according to the present invention.
(a) and a center line sectional view (b).

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ISFET base 2 Exterior part 3 Depression 4 Insulating ion sensitive part 5 Organic material film 6 Adhesive

Claims (5)

[Claims] An insulative ion sensitive part of an ion sensitive field effect transistor is formed with an organic material film capable of converting a substance to be measured in urine into a substance sensitive to the insulative ion sensitive part. A urine substance measuring instrument provided with a storage structure. 2. The urine substance measuring instrument according to claim 1, wherein the organic substance film capable of reacting the substance to be measured in urine with the insulating ion sensitive part is an enzyme film, an antibody film or a complex film. 3. A urine pH measuring device having a structure in which urine is accumulated around an insulating ion-sensitive portion of an ion-sensitive field-effect transistor. 4. The urine substance measuring instrument according to claim 1, or the urine pH measuring instrument according to claim 2, which is attached to a position in a toilet that comes into contact with urine. 5. The measurement result of at least one of the urine substance measuring device according to claim 1 and the urine pH measuring device according to claim 2 is sent to a display unit by an electromagnetic wave, and the measurement result is displayed there. Urine measurement system.