JPH02286165A - Deodorant - Google Patents

Abstract

PURPOSE:To obtain the deodorant applicable for intraoral use in a wide range by using the high-polymer fraction of a specific mol.wt. obtd. by protease decomposition of separated soy bean protein as the effective component. CONSTITUTION:Dry green soy beans are roughly ground and after the skin is removed therefrom, the soy beans are further ground to <=30 mesh powder. The powder is degreased by using n-hexane or a soln. mixture composed of methanol and chloroform as a solvent and is dissolved by a weakly alkaline soln. The soln. is subjected to a solid-liquid sepn. by a centrifugal sepn. or the like by which the undissolved matter is removed. The pH of the supernatant liquid thereof is controlled to weak acidity to allow the protein to settle and the protein is subjected to the solid-liquid sepn. by the centrifugal sepn. or the like. The separated solid is freeze-dried after washing with water and is separated by which the soy bean protein is obtd. This protein is sufficiently decomposed by the protease and after the oxygen is deactivated, the protein is subjected to the solid-liquid sepn. by the centrifugal sepn. or the like. The residues thereof are dissolved again in the alkaline soln. of the weak alkalinity and <100000 fractions are removed by an ultrafilter membrane of 100000mol.wt. The remaining soln. of >=100000 fractions is subjected to isoelectric point precipitation to recover the protein. After the protein is thoroughly washed with water, the protein is freeze- dried.

Description

DETAILED DESCRIPTION OF THE INVENTION (Field of Industrial Application) The present invention relates to a deodorant that exhibits a good deodorizing effect on both hydrophilic and hydrophobic sulfur-based odor components.

(Conventional technology) Generally, sulfur compounds are well known as a source of bad odors.

For example, this applies to the odors that often occur in craft manufacturing factories, livestock farms, urine treatment plants, fish ilium treatment plants, and the like.

In addition, sulfur compounds are also the main components of bad breath, deep in the stomach, and inside garlic, which causes discomfort.

Therefore, many proposals have been made to date as deodorants for malodorous substances such as these sulfur-based odor components.

For example, activated carbon, zeolite, cyclodextrin, which adsorbs and clathrates odor components to make them physically odorless, various reagents such as oxidizer solutions, acid solutions, and chilkali solutions that decompose odor components through chemical reactions, or strong aroma components. There are various fragrances and the like that act as masking agents that make it difficult to perceive odors sensually.

By the way, with regard to deodorants contained in foods, toothpaste, mouthwash, etc. for the purpose of removing bad breath, standards have been established with particular consideration to safety for the human body, so it is not possible to use them. Materials are limited.

As an oral deodorant (useful compounds include fragrance compounds, cyclodextrin, chlorophyll, flavonoids, etc.).

However, cyclodextrin (hereinafter referred to as CD) is a fragrance compound that sensually numbs the sense of smell and makes it difficult to perceive odors due to the strong sting of the fragrance, and is not an essential deodorizer. The enzyme cyclomaltodextrin glucanotransferase (Oyc) is added to starch.
lomaltodextringlucanotran
aferase), and glucose is
-1,4 bond to form a ring. As types of CD, α-(3D consisting of 6 glucose units, β-CD consisting of 7 units, j-CD consisting of 8 units, etc.) are known. These CDs have a hydrophobic cyclic structure, Since CD has the property of taking substances into the intramolecular cavity and clathrating them, it has the effect of suppressing odor components.Also, since CD is almost tasteless and odorless, it can be applied to a wide range of foods.However, if the OD cavity The types of odor components that can be included are limited because there are restrictions on the properties, three-dimensional structure, and molecular weight of the molecules that can invade.Also, since inclusion is sufficient and sexual, hydrophobic CD
Hydrophobic sulfur compounds such as diallyl disulfide are difficult to be included in the ring, but hydrophobic sulfur compounds such as diallyl disulfide are easily included.

In addition, chlorophyll and flavonoids also exist on their own,
Also, its deodorizing effect is weaker than that of cyclodextrin.

As mentioned above, the reality is that conventional oral deodorants are inadequate in either the applicable product range or the types of odor components that can be deodorized.

(Problem to be solved by the invention Ml) The present invention was made in view of the above circumstances, and
The purpose of this product is to have a high deodorizing effect against both hydrophilic and hydrophobic sulfur-based odor components, and the deodorant itself is tasteless, odorless, and colorless, making it highly safe and suitable for use in the oral cavity. The purpose of the present invention is to provide a deodorant that can be applied in a wide range of applications.

(Means for Solving the Problem) The above object is achieved by a deodorant containing as an active ingredient a polymer fraction having a molecular weight of 100,000 or more obtained by decomposing isolated soybean protein with protease.

That is, in order to couple the above objectives, the present inventors conducted intensive studies and found that a molecule obtained by protease decomposition of soybean fraction l:a protein [more than 100,000 polymer fractions contain sulfur] The present invention was completed based on the discovery that it has an excellent deodorizing effect on odor components, and is almost tasteless, odorless, and colorless, making it suitable as an oral deodorant.

The present invention will be explained in detail below.

The isolated soybean proteolytic polymer fraction, which is the active ingredient of the deodorant of the present invention, can be produced, for example, as follows. That is, first, dry raw soybeans are roughly ground, the skins are removed, and then the powder is further made into a powder of sO Messi A or lower. This is n-
Degrease using hexane or a methanol/chloroform mixture as a solvent. The obtained defatted soybeans are dissolved in a weakly alkaline alkaline solution, solid-liquid separation is performed by centrifugation, etc., and undissolved substances are removed. Adjust the pH of the rice supernatant to slightly acidic, precipitate the protein, separate the solid-liquid layer by centrifugation, wash with water and dry for 1 minute to obtain soybean protein.

Next, the separated soybean protein from the kernel is sufficiently decomposed with protease, and after the enzyme is inactivated, solid-liquid separation is performed by centrifugation or the like to obtain a residue.

This residue was dissolved again in a slightly alkaline alkaline solution,
A fraction with a molecular weight of less than 100,000 is removed using an ultrafiltration membrane with a molecular weight of 100,000.

The remaining 100,000 or more solutions were subjected to isoelectric precipitation,
If the protein is collected, thoroughly washed with water, and then freeze-dried, a powdered separated soybean protein polymer fraction can be obtained.

Various proteases can be used as the protease mentioned above, including Aspergillus (Aapergillus R. Aspergillus or Bacillus subtilis).
From the viewpoint of flavor, it is preferable to use endo- and exo-type proteases produced by microorganisms originating from Bacillus subtilis.

The deodorant of the present invention obtained as described above has hydrophilicity,
It is hydrophobic and exhibits an excellent deodorizing effect against all sulfur-based odor components. These sulfur-based odor components include hydrogen sulfide, methyl mercaptan, ethyl mercaptan, lower alkyl and alkenyl sulfides, disulfides, trisulfides, etc. Examples include hydrophilic methyl mercaptan, which is a putrid odor, and garlic odor. One example is hydrophobic diallyl disulfide.

The deodorant of the present invention is pale in color and almost white,
There is almost no taste or odor, and there are no problems with product formulation. Furthermore, since it is derived from soybean protein, it is highly safe to use.

Although the deodorant of the present invention is sparingly soluble in water, it can be prepared into a desired form such as a suspension, paste, powder, or block. In this case, the deodorant of the present invention maintains its deodorizing effect very stably in various forms such as solid state and aqueous solution state, and can be formulated in various forms.

Due to the above-mentioned features, the deodorant of the present invention has - general food (
For example, it can be used as an oral deodorizer that is incorporated into confectionery such as gum), toothpaste, mouthwash, and the like.

At this time, the compounder of the deodorant of the present invention preferably uses 0.001 to sO weight % (hereinafter referred to as %), more preferably 0.1 to 10%, to the entire product. Furthermore, the deodorizer of the present invention is not limited to oral deodorizers, but is also suitable for garbage, refrigerators, car interiors, toilets, and sewage treatment plants, as well as works that generate volatile odors. It exhibits excellent deodorizing ability against odor components such as amines and lower fatty acids, but it is suitable to use it in combination with other deodorants such as β-0D1 flavonoids and chlorophyll. It is.

(Effects of the Invention) The deodorant of the present invention contains as an active ingredient a polymer fraction having a molecular weight of 100,000 or more obtained by decomposing isolated soybean protein with protease.

As mentioned above, this polymer fraction exhibits an excellent deodorizing effect against both hydrophilic and hydrophobic sulfur-based odor components such as methyl mercaptan and diallyl disulfide, which is the main component of garlic odor.

Furthermore, since it is a tasteless, odorless, and colorless powder, it poses few problems in product formulation and is highly safe to use.

Therefore, it can be used not only as a deodorant for the oral cavity but also as a deodorant for various sulfur-based odor components.

The present invention will be specifically described below with reference to Examples.

(Example) First, dry raw soybeans are roughly crushed, the skin is removed, and then
It was made into a powder with less than 0 mesh. This was defatted by refluxing in a flask at 80 to 60°C for 5 hours using methanol/chloroform (=1/1 mixed solution 1) to obtain defatted soybeans (Yo 1).The resulting defatted soybeans had a pH of 9. The protein was dissolved in an aqueous solution of sodium hydroxide, separated into solid and liquid by centrifugation, and undissolved matter was removed. Solid-liquid separation was performed, washed with water, and freeze-dried to obtain isolated soybean protein (N[L2).

Next, the thus obtained isolated soybean protein 5o.

g was dispersed in phosphate buffer 501 at pH 7.0, and 10 g of a protease preparation derived from Bacillus subtilg containing endo-type and exo-type proteases (manufactured by Nagase Sangyo Co., Ltd., Biobrase 8p-4) was added, The reaction was carried out at 40°C for 10 hours. After the reaction, heat at 86°C for 20 minutes to inactivate the enzyme,
The solution was centrifuged (10,000 rpm x 10 minutes) to obtain a supernatant solution and a residue.

This residue was thoroughly washed with water and then freeze-dried to obtain a powder of about 75.1%. This freeze-dried powder was dissolved in a pH II sodium hydroxide aqueous solution, and the molecular size was reduced to 100,000 or more, less than 100,000 to 20,000 or more, less than 20,000 using ultrafiltration membranes (manufactured by Sartorius) with molecular ff110,000 and 20,000. Fractionated. The fractions were subjected to isoelectric precipitation, centrifuged, thoroughly washed with water, and freeze-dried to form a powder. The yield of each powder is 84.81 (Nhj), o, asf (m4), 0, respectively.
．． 12511t8). In addition, the supernatant solution was freeze-dried. (Also, using the above isolated soybean protein, Aspergillus oryzae (Aspergillus oryzae) was used as a protease.
The same procedure as above was performed using a protease originating from A. After decomposition, centrifuge to separate the supernatant solution and residue, and use an ultrafiltration membrane to separate the residue into 100,000 or more, less than 100,000 to 20,000, and less than 20,000 in the same manner as above to obtain a powder. Ta. The respective yields are 14.3f (Nu1), 11F
(positive 8), 0.56y (positive 8). The supernatant solution was lyophilized. (Nllll O) Above sample No.1
~N010 and β-CD (11&111) were compared in terms of deodorizing power using the headspace method. That is, the above sample s is placed in a vial with an internal capacity of 20 m1.
Add omy and 1ml of water, and add 20μ of methyl mercaptan solution (1μfl/met ethanol solution) to this.
e Or diallyl disulfide solution (50 μtt
Add 20 μl of ethanol solution), immediately cover with a rubber stopper, stir vigorously for 10 minutes, and incubate at 25°C for 30 minutes.
It was left standing for a minute. After that, collect 0.4ml of headspace gas in the vial with a gastight syringe,
Immediately, the sample was injected into a gas chromatograph (14 people from Shimadzu) and the peak height count (8) of the sample was measured. The case where no sample was added was used as the peak height count (of the control), and the deodorization rate was calculated from the following formula.

C: Control peak height count 8: Sample peak height count The above operation was performed for each sample.

These results are shown in Table 1.

Qin) Examples of year-end inventions.

Also, in order to see the sensual deodorizing effect of each sample of the above Examples and Comparative Examples,! Evaluation was performed using criteria such as the g2 table. That is, methyl mercaptan solution (1μI/m
e, ethanol solution) and diallyl disulfide solution (
60μI/mL ethanol solution) was used as the target odor component, and the above sample s o o
mp was added, and 10 panelists added 6 as shown in Table 2.
The odor intensity was sensory evaluated in stages, and the average value was calculated.

In addition, odor intensity when no sample is added (blank)
was set as 4. Each sample was evaluated for two types of odor components. Table 5 shows the results.

Table 2 Table 3 1M) Examples of year-end inventions As described above, the deodorant active ingredient of the present invention has a high deodorization rate and a high deodorization rate against sulfur compounds such as hydrophilic methyl mercaptan and hydrophobic diallyl disulfide. It showed excellent deodorizing effect in both sensory evaluations. In particular, it showed far superior deodorizing power compared to β-OD, which is widely used as a deodorizing agent in ordinary foods.

In addition, the deodorant of the present invention is made from soybean protein, is almost colorless, tasteless, and odorless, and poses no danger to the human body, so it can be used in various forms in various products. I can do it.

An example of its use is shown below.

[Usage example 1] Chewing gum gum paste 20% sugar 64 glucose 6 water m 20 apple flavor
Ols total too, o% With the above formulation, chewing gum was prepared according to a conventional method, and 7
It was molded into a size of 2 x 19 x 1.9 m/m to obtain a deodorizing gum. This gum is a sheet gum with an apple flavor, and the deodorant of the present invention was able to maintain the original quality of the gum without affecting the flavor, texture, or color tone of the gum.

[Usage example 2] Tablet confectionery powder Sugar 80% glucose
11.5 Citric acid 2 Total 10 [Usage example S] Toothpaste calcium hydrogen phosphate 4 Propylene glycol sorbitol! Carboxymethyl cellulose sodium colloidal silica lauryl sulfate 0.0% 0.0% 2.0 1.1! . 0 1.6 Sodium monofluorophosphate 0.16 Water remaining 100.0% [Usage example 4] Mouth wash ethanol 20% Sodium lauryl sulfate 1 Fragrance 1 Glycerin
10 Sodium hydrogen phosphate 0.5 Water Total remaining 100.0% As mentioned above, the deodorant of the present invention itself is almost tasteless, odorless, and colorless, and exhibits a deodorizing effect in a small amount. It was possible to produce rice products using conventional production methods without affecting the quality of various products such as foods.

Claims (1)

[Claims] Molecular weight 1 obtained by decomposing isolated soybean protein with protease
A deodorant containing a polymer fraction of 0,000 or more as an active ingredient.