JPH01218698A - Denitrifying method - Google Patents
Denitrifying methodInfo
- Publication number
- JPH01218698A JPH01218698A JP4726188A JP4726188A JPH01218698A JP H01218698 A JPH01218698 A JP H01218698A JP 4726188 A JP4726188 A JP 4726188A JP 4726188 A JP4726188 A JP 4726188A JP H01218698 A JPH01218698 A JP H01218698A
- Authority
- JP
- Japan
- Prior art keywords
- nitrogen
- yeast
- nitrite
- filamentous fungi
- denitrification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 35
- 241000233866 Fungi Species 0.000 claims abstract description 20
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 17
- 239000000758 substrate Substances 0.000 claims abstract description 12
- 241000223218 Fusarium Species 0.000 claims abstract description 8
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims abstract description 4
- 241000235648 Pichia Species 0.000 claims abstract description 4
- 241000235527 Rhizopus Species 0.000 claims abstract description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 20
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims description 16
- 244000005700 microbiome Species 0.000 claims description 4
- 241000221960 Neurospora Species 0.000 claims description 2
- 241000894006 Bacteria Species 0.000 abstract description 7
- 239000002351 wastewater Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 229910002651 NO3 Inorganic materials 0.000 description 4
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 4
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000029058 respiratory gaseous exchange Effects 0.000 description 4
- 239000010865 sewage Substances 0.000 description 4
- 241000206602 Eukaryota Species 0.000 description 3
- 241000223221 Fusarium oxysporum Species 0.000 description 3
- 150000003863 ammonium salts Chemical class 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000001925 catabolic effect Effects 0.000 description 3
- 239000010842 industrial wastewater Substances 0.000 description 3
- 125000001477 organic nitrogen group Chemical group 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 230000034303 cell budding Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- IIPYXGDZVMZOAP-UHFFFAOYSA-N lithium nitrate Chemical compound [Li+].[O-][N+]([O-])=O IIPYXGDZVMZOAP-UHFFFAOYSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000588264 Rhizopus javanicus Species 0.000 description 1
- 240000005384 Rhizopus oryzae Species 0.000 description 1
- 235000013752 Rhizopus oryzae Nutrition 0.000 description 1
- MUOWPGBTEYOCTP-UHFFFAOYSA-J [Th+4].[O-]N=O.[O-]N=O.[O-]N=O.[O-]N=O Chemical compound [Th+4].[O-]N=O.[O-]N=O.[O-]N=O.[O-]N=O MUOWPGBTEYOCTP-UHFFFAOYSA-J 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 238000004993 emission spectroscopy Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002808 molecular sieve Substances 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 150000002826 nitrites Chemical class 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 235000010288 sodium nitrite Nutrition 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- -1 wastewater Chemical compound 0.000 description 1
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
Description
【発明の詳細な説明】
童深上の利用分野
本発明は、硝酸態窒素又は亜硝酸態窒素の脱窒方法に係
り、特に、廃水中に含有される硝酸態窒素又は亜硝酸態
窒素を還元して、カス状の窒素として除去する脱窒方法
に関する。DETAILED DESCRIPTION OF THE INVENTION Field of Application of Dosuka The present invention relates to a method for denitrifying nitrate nitrogen or nitrite nitrogen, and in particular to a method for denitrifying nitrate nitrogen or nitrite nitrogen contained in wastewater. The present invention relates to a denitrification method in which nitrogen is removed in the form of scum.
Ｕ９１月仔
自然界における窒素循環システムについては研究かかな
り行われ、有機態窒素やアンモニウム塩が、最終的には
、好気的条件下で、硝化細菌により亜硝酸態窒素及び硝
酸態窒素に酸化され、また、この亜硝酸態窒素及び硝酸
態窒素が、嫌気的条件下に通性嫌気性の脱窒素菌により
、ガス状の窒素に還元されることは良く知られている。Considerable research has been conducted on the nitrogen circulation system in nature, and it has been shown that organic nitrogen and ammonium salts are ultimately oxidized to nitrite nitrogen and nitrate nitrogen by nitrifying bacteria under aerobic conditions. It is also well known that nitrite nitrogen and nitrate nitrogen are reduced to gaseous nitrogen by facultative anaerobic denitrifying bacteria under anaerobic conditions.
近年、水域における富栄養化の進行及びこれによる藻や
赤潮の異常発生にともない、廃水中の窒素の除去プロセ
スとして、上記窒素循環システムの一部を利用する試み
が多々なされている。ずなわぢ、先ず、都市下水や工場
廃水等を、好気的条件下に活性汚泥と接触させ、有機物
の酸化とともに、有機態窒素及びアンモニウム塩を、亜
硝酸態窒素及び硝酸態窒素に酸化し、次いで、嫌気的条
件にし、亜硝酸態窒素及び硝酸態窒素を還元、脱窒する
方法が行われている。In recent years, with the progress of eutrophication in water bodies and the resulting abnormal occurrence of algae and red tide, many attempts have been made to utilize part of the nitrogen circulation system as a process for removing nitrogen from wastewater. First, urban sewage or industrial wastewater is brought into contact with activated sludge under aerobic conditions to oxidize organic matter and oxidize organic nitrogen and ammonium salts to nitrite nitrogen and nitrate nitrogen. Next, a method of reducing and denitrifying nitrite nitrogen and nitrate nitrogen under anaerobic conditions has been carried out.
尚、上記脱窒素作用は、原核生物であるバクテリアでは
知られているか、真核生物では、全く知られていない。The denitrification effect described above is known in bacteria, which are prokaryotes, or is completely unknown in eukaryotes.
なお、従来、上記脱窒の際に作用するｊｍ性嫌気性の脱
窒素菌は、好気性菌等に比べて大きなフロックかでき何
１く、したがって、脱窒後の菌体の分離が何ましいとい
う問題がある。Conventionally, the JM anaerobic denitrifying bacteria that act during denitrification have been unable to form large flocs compared to aerobic bacteria, and therefore the separation of bacterial bodies after denitrification has been difficult. There is a problem.
発明が解決しようとする課題
本発明は、−）二記問題点を解決して、硝酸態窒素又は
亜硝酸態窒素を含有する基質、例えば廃水中のこれら窒
素化合物を微生物を利用して還元し、ガス状の窒素とし
て除去するための脱窒方法を提供することを課題とする
。Problems to be Solved by the Invention The present invention solves the problems described in -) and reduces nitrogen compounds in substrates containing nitrate nitrogen or nitrite nitrogen, such as wastewater, using microorganisms. An object of the present invention is to provide a denitrification method for removing nitrogen as gaseous nitrogen.
裸苅玉邂扱−几友屹ｑ下段
本発明者は、植物病原菌である数種の糸状菌の代謝研究
を進めていた過程において、培地中に硝酸塩や亜硝酸塩
を添加し、通気を抑えて前記糸状菌を培養すると、異化
型の硝酸、亜硝酸呼吸を行うこと及び酵母についても同
様の作用があることを見い出した。かかる異化型の硝酸
、亜硝酸呼吸は、前述したように真核生物においては全
く知られていなかった。Handling naked calley balls - 几ゆ屹q lower row In the process of conducting metabolic research on several types of filamentous fungi that are plant pathogens, the present inventor added nitrates and nitrites to the culture medium to suppress aeration. It was discovered that when the filamentous fungus is cultured, it performs catabolic nitrate and nitrite respiration, and that yeast also has a similar effect. As mentioned above, such catabolic nitrate and nitrite respiration was completely unknown in eukaryotes.
本発明は、このような知見に基づきなされたもので、本
発明は、硝酸態窒素又は亜硝酸態窒素を含有する基質を
嫌気性条件下に糸状菌または酵母と接触させ、脱窒素す
ることから構成されるものである。尚、この発明におい
ては、リゾプス属、ギベレラ属、フザリウム属、ハンセ
ヌラ属に属する微生物から選択された菌株を用いること
が好ましい。The present invention was made based on such knowledge, and the present invention involves bringing a substrate containing nitrate nitrogen or nitrite nitrogen into contact with filamentous fungi or yeast under anaerobic conditions to denitrify the substrate. It is composed of In the present invention, it is preferable to use a strain selected from microorganisms belonging to the genus Rhizopus, Gibberella, Fusarium, and Hansenula.
本発明にいう糸状菌とは、菌糸からなり、当該菌糸が分
岐して菌糸体を形成し、葉緑素を有しない一群の生物を
指すが、生物分類学上では、菌類の中の真菌類を構成す
る接合菌類、子嚢菌類、担子菌類および不完全菌類の４
綱のうちで、主として出芽によって無性生殖を行ういわ
ゆる酵母を除外した部分で、しかも体が糸状を呈する一
群の微小な生物を指すものである。この糸状菌の中でも
、リゾプス（Ｒｈｉｚｏｐｕｓ）属、ギベレラ（Ｇｉｂ
ｂｅｒｅｌ Ｉａ）属、フザリウム（Ｆｕｓａｒｉｕｍ
）属、ニューｒ′−１スポラ（Ｎｅｕｒｏｓｐｏｒａ）
属等を好適に用いることができる。The term filamentous fungi as used in the present invention refers to a group of organisms that consist of hyphae, which branch to form mycelium, and do not have chlorophyll; 4 of the Zygomycetes, Ascomycetes, Basidiomycetes and Deuteromycetes
This class excludes so-called yeast, which reproduce asexually through budding, and refers to a group of microscopic organisms with filamentous bodies. Among these filamentous fungi, Rhizopus spp., Gibberella spp.
berel Ia) genus, Fusarium
), genus Neurospora
The genus etc. can be suitably used.
−・方、酵母は、菌類のなかで栄養細胞が出芽によって
増えるもので、有性生殖を行う有胞子酵母及び有性生殖
を行わない無胞子酵母を含むものである。この酵母の中
で、ハンセヌラ（ｌｌａｎｓｅｎｕｌａ）属等を好適に
用いることができる。On the other hand, yeast is a type of fungi in which vegetative cells multiply by budding, and includes spore-bearing yeast that reproduce sexually and non-spore yeast that do not reproduce sexually. Among these yeasts, the genus Hansenula and the like can be suitably used.
以上のような糸状菌又は酵母の菌株の一部は、第１表に
記載する受理番号で工業技術院微生物工業技術研究所（
ＦＥＲＭ）に寄託され、また、表に記載する菌株番号に
より、叫）発酵研究所（ＩＦＯ）、東京大学応用微生物
研究所（ｒＡＭ）から、当該技術分野におりる通常の知
識を有するものが容易に入手できるようになっている。Some of the filamentous fungi or yeast strains mentioned above have been reported to the National Institute of Microbiology, Agency of Industrial Science and Technology (National Institute of Microbial Technology, Agency of Industrial Science and Technology) with the accession numbers listed in Table 1.
FERM) and the strain numbers listed in the table can be easily identified by those with ordinary knowledge in the technical field from the Institute for Fermentation Research (IFO) and the Research Institute for Applied Microbiology (rAM), the University of Tokyo. It is now available.
第 １ 表
また、寄託微生物であるフザリウム・オキシスポラムの
菌学的性質は、１’、５ａＬｏｈ ｅｔ ａｌ、、アグ
リカルチュラルアン１〜ハイオロジカルケミストリイ
〔八（４ｒ、 Ｂｉｏｌ、 Ｃｈｃｍ、、４０．９５３
−９６１（１９７６） ）に詳記されている。Table 1 The mycological properties of Fusarium oxysporum, the deposited microorganism, are as follows: 1', 5aLoh et al.
[8 (4r, Biol, Chcm, 40.953
-961 (1976)).
本発明においては、上記糸状菌及び酵母を利用して、硝
酸態窒素又は亜硝酸態窒素を資化させるが、これらの窒
素は、専ら、イオンあるいは塩の形態で用いる。この硝
酸態窒素又は亜硝酸態窒素の濃度は、用いる糸状菌や酵
母の種類、濃度等により一概には決めらないが、１００
〜５０００ｐｐｍとすることが好ましい。また、基質中
には、これらの窒素以外に炭素源及び無機塩を、適宜添
加する。In the present invention, the above-mentioned filamentous fungi and yeast are used to assimilate nitrate nitrogen or nitrite nitrogen, and these nitrogens are used exclusively in the form of ions or salts. The concentration of nitrate nitrogen or nitrite nitrogen cannot be determined unconditionally depending on the type of filamentous fungus or yeast used, the concentration, etc.
It is preferable to set it as 5000 ppm. In addition to nitrogen, a carbon source and an inorganic salt are appropriately added to the substrate.
基質に対する糸状菌や酵母の濃度は、培地の形態、資化
方式等を勘案し、適宜選定される。尚、培地は、固体で
も、液体でも良く、特に、廃水処理に、本発明を適用す
る場合は、糸状菌或いは酵母の生育に必要な基質で、廃
水中に含まれていないものを添加すれば良い。The concentration of filamentous fungi and yeast relative to the substrate is appropriately selected in consideration of the form of the medium, assimilation method, etc. The medium may be either solid or liquid, and in particular, when the present invention is applied to wastewater treatment, a substrate necessary for the growth of filamentous fungi or yeast that is not contained in wastewater may be added. good.
糸状菌又は酵母による資化は、バクテリアによる脱窒と
比べ、より好気的であるので、雰囲気を高度の嫌気条件
にする必要もなく、空気とすることも可能である。また
、この資化は、ｐＨ５〜９．０〜５０℃の温度で行われ
、通常は、常圧下で行うが、加圧下で行ってもよい。Assimilation by filamentous fungi or yeast is more aerobic than denitrification by bacteria, so there is no need to make the atmosphere highly anaerobic, and air can be used. Moreover, this assimilation is carried out at a temperature of pH 5 to 9.0 to 50°C, and is usually carried out under normal pressure, but may be carried out under increased pressure.
資化方式は、回分式、連続式、半回分式のいずれでも良
いが、特に廃水処理に適用する場合は、前記糸状菌及び
酵母を固定し、いわゆるバイオリアクタ一方式で行うと
効率が良く、特に好ましい。The assimilation method may be batch, continuous, or semi-batch, but especially when applied to wastewater treatment, it is more efficient to fix the filamentous fungi and yeast and use a so-called bioreactor one-way method. Particularly preferred.
尚、都市下水や工場廃水等の処理に本発明を適用する場
合は、先ず、これらの廃水を好気的条件下に活性汚泥と
接触させ、を機動の酸化とともに、有機態窒素及びアン
モニウム塩を、亜硝酸態窒素及び硝酸態窒素に酸化し、
次いで、この処理水を、糸状菌又は酵母により、亜硝酸
態窒素及び硝酸態窒素を還元、脱窒する方法を採ると良
い。When applying the present invention to the treatment of urban sewage, industrial wastewater, etc., first, these wastewaters are brought into contact with activated sludge under aerobic conditions to oxidize the wastewater and remove organic nitrogen and ammonium salts. , oxidizes to nitrite nitrogen and nitrate nitrogen,
Next, it is preferable to adopt a method in which nitrite nitrogen and nitrate nitrogen are reduced and denitrified from this treated water using filamentous fungi or yeast.
以下実施例により、本発明及びその効果を具体的に説明
する。The present invention and its effects will be specifically explained below with reference to Examples.
実施例１
三つ口の３００ｍβ容三角フラスコに、第２表に示した
組成に亜硝酸すトリウムまたは硝酸す１〜リウムを０．
５％の濃度で加えた液体培地１８０ｍｆｆを入れ、これ
に前記第１表に記載した糸状菌又は酵母をそれぞれ、お
およそ１ｇ−湿潤菌体／βの濃度になるように接種し、
内部を二酸化炭素で置換した後、ゴム栓をした。２６．
５℃の温度で、ロータリーシェーカーを用いて振とう培
養し、所定時間経過に上部気体を０．５ｍ７！づつり“
ンプリングし、ガスクロマトグラフィーにより窒素ガス
の濃度を測定した。Example 1 In a three-necked 300 mβ Erlenmeyer flask, 0.00% of thorium nitrite or 1 to 30% of lithium nitrate was added to the composition shown in Table 2.
Add 180 mff of a liquid medium added at a concentration of 5%, inoculate each of the filamentous fungi or yeast listed in Table 1 above to a concentration of approximately 1 g - wet bacterial cells/β,
After replacing the inside with carbon dioxide, a rubber stopper was placed. 26.
Shake culture using a rotary shaker at a temperature of 5°C, and after a predetermined period of time, add 0.5 m7 of upper gas! Zitsuri “
The concentration of nitrogen gas was measured by gas chromatography.
この結果を第３表に示す。The results are shown in Table 3.
尚、培養開始時の酸素量は１５容量％であった。Note that the oxygen amount at the start of culture was 15% by volume.
第２表 培地組成 （水道水）
実施例２
実施例１の第２表と同様な組成に原子ｉ１５の安定同位
体窒素を含む亜硝酸ナトリウムまたは硝酸ナトリウムを
０．５％濃度で加えた液体培地に、実施例１で用いたフ
ザリウム・オキシスポラム、リヅブス・オリザエ、リヅ
プス・ジャバニカスの菌株を接種し、同様に培養した。Table 2 Medium composition (tap water) Example 2 Liquid medium with the same composition as in Table 2 of Example 1 with the addition of sodium nitrite or sodium nitrate containing the stable isotope nitrogen of atom i15 at a concentration of 0.5% The strains of Fusarium oxysporum, Rhizopus oryzae, and Rhizopus javanicus used in Example 1 were inoculated and cultured in the same manner.
培養７日後に、上部のガス中の窒素を、液体窒素で冷却
したモレキュラーシーブにトラップした後、放電管に導
き、窒素中の原子量１５の同位体の量を発光分光法によ
り測定した。原子量１５の同位体の星は、全窒素に対し
、フザリウム・オキシスポラムの場合は、６５％、他は
、１０％であった。このことから、糸状菌及び酵母が、
異化型の硝酸、亜硝酸呼吸をしていることが分かる。After 7 days of culture, the nitrogen in the upper gas was trapped in a molecular sieve cooled with liquid nitrogen, then introduced into a discharge tube, and the amount of isotope with an atomic weight of 15 in nitrogen was measured by emission spectroscopy. The isotopic stars with an atomic mass of 15 accounted for 65% of the total nitrogen in Fusarium oxysporum and 10% in the others. From this, filamentous fungi and yeast
It can be seen that they respire catabolic nitrate and nitrite.
以上の結果から明らかなように、真核生物である糸状菌
又は酵母が異化型の硝酸、亜硝酸呼吸を行い、これを用
いることにより、硝酸態窒素又は亜硝酸態窒素を含む基
質、例えば、都市下水や工場廃水中の脱窒素を行うこと
ができることは明らかである。As is clear from the above results, filamentous fungi or yeast, which are eukaryotes, perform dissimilatory nitrate and nitrite respiration, and by using this respiration, they can produce substrates containing nitrate nitrogen or nitrite nitrogen, for example. It is clear that denitrification of municipal sewage and industrial wastewater can be carried out.
発明の効果
本発明は、硝酸態窒素又は亜硝酸態窒素を含有する基質
を糸状菌又は酵母で資化させ、脱窒素するため、菌体の
分離が容易であり、都市下水や］−場廃水中の脱窒素を
効率良く行うことができるという効果を有する。Effects of the Invention In the present invention, a substrate containing nitrate nitrogen or nitrite nitrogen is assimilated by filamentous fungi or yeast and denitrified. Therefore, it is easy to separate the bacterial cells, and it can be used in urban sewage and field wastewater. This has the effect of efficiently denitrifying the inside.
Claims (2)
これら窒素の資化能を有する糸状菌又は酵母で資化させ
、脱窒素することを特徴とする脱窒方法。(1) Substrate containing nitrate nitrogen or nitrite nitrogen,
A denitrification method characterized in that nitrogen is assimilated by filamentous fungi or yeast having the ability to assimilate nitrogen.
ゾプス属、ギベレラ属、フザリウム属、ニューロスポラ
属、ハンセヌラ属に属する上記窒素の資化能を有する微
生物で資化させ、脱窒素することを特徴とする脱窒方法
。(2) Substrates containing nitrate nitrogen or nitrite nitrogen are denitrified by assimilation by microorganisms that are capable of assimilating the above nitrogen and belong to the genus Rhizopus, Gibberella, Fusarium, Neurospora, and Hansenula. A denitrification method characterized by:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63047261A JP2513495B2 (en) | 1988-02-29 | 1988-02-29 | Denitrification method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63047261A JP2513495B2 (en) | 1988-02-29 | 1988-02-29 | Denitrification method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01218698A true JPH01218698A (en) | 1989-08-31 |
| JP2513495B2 JP2513495B2 (en) | 1996-07-03 |
Family
ID=12770345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63047261A Expired - Lifetime JP2513495B2 (en) | 1988-02-29 | 1988-02-29 | Denitrification method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2513495B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006239583A (en) * | 2005-03-03 | 2006-09-14 | Mie Univ | Sintered body for water purification, and production method |
| JP2018176127A (en) * | 2017-04-20 | 2018-11-15 | 有限会社オフイスヨコオ | Method for removing nitrate nitrogen and nitrate nitrogen remover |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5351657A (en) * | 1976-10-20 | 1978-05-11 | Kuraray Co Ltd | Method for disposing organic waste water |
| JPS5458951A (en) * | 1977-10-19 | 1979-05-12 | Asahi Carbon Co Ltd | Method of disposing starch and*or saccharoid containing waste water |
| JPS54154162A (en) * | 1978-05-19 | 1979-12-05 | Ici Ltd | Disposal method of waste liquor formed from processing of milks or production of dairy products |
-
1988
- 1988-02-29 JP JP63047261A patent/JP2513495B2/en not_active Expired - Lifetime
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5351657A (en) * | 1976-10-20 | 1978-05-11 | Kuraray Co Ltd | Method for disposing organic waste water |
| JPS5458951A (en) * | 1977-10-19 | 1979-05-12 | Asahi Carbon Co Ltd | Method of disposing starch and*or saccharoid containing waste water |
| JPS54154162A (en) * | 1978-05-19 | 1979-12-05 | Ici Ltd | Disposal method of waste liquor formed from processing of milks or production of dairy products |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2006239583A (en) * | 2005-03-03 | 2006-09-14 | Mie Univ | Sintered body for water purification, and production method |
| JP2018176127A (en) * | 2017-04-20 | 2018-11-15 | 有限会社オフイスヨコオ | Method for removing nitrate nitrogen and nitrate nitrogen remover |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2513495B2 (en) | 1996-07-03 |
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