JPH0995448A - Increase in biotin concentration in blood and biotin-containing beverage or food product - Google Patents

Abstract

PROBLEM TO BE SOLVED: To increase the concentration of biotin in blood in order to synergistically increase the absorption of biotin useful for prevention of obesity, diabetes, dermatitis, conjunctivitis, muscle ache and feeling of fatigue into blood. SOLUTION: Intestinal flora conditions (environments) are adjusted with oligosaccharides and simultaneously biotin is ingested to increase the concentration of biotin in blood. The biotin used is available from naturally occurring substances such as cereals, internal organs of live-stocks, egg yolk and cell bodies of microorganisms or from chemically synthetic substances mainly used as medicines. The oligosaccharides and biotin is preferably taken everyday continuously in effective, and the content of biotin in the food or beverage is preferably that corresponding to 30-300μg/day.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a method for increasing blood biotin concentration by ingesting biotin at the same time as oligosaccharides, and a biotin-containing food or drink having an action of increasing blood biotin concentration.

[0002]

2. Description of the Related Art In modern diets, foods whose biotin content is remarkably reduced during processing are frequently used, and biotin intake tends to be insufficient. In addition, as symptoms of biotin deficiency, dermatitis, conjunctivitis, myalgia, tiredness, elevation of blood glucose level, etc. are known.

The content of biotin is drastically reduced due to the processing and cooking of food materials, and the modern diet in which processed foods are heavily used is, so to speak, a low biotin intake lifestyle, with the potential risk of biotin deficiency. There is. Adults are not known to have biotin deficiency, but infants have biotin deficiency symptoms. The causes of sudden infant death are:
The association with stress under biotin deficiency has been pointed out. Since the amount of biotin has decreased in the process of manufacturing infant formula, WHO has recommended adding biotin to infant formula for decades.

Damage to biotin absorption by egg white avidin in humans has been known for a long time. However, it is said that in general healthy adults, biotin that meets the required amount is produced by intestinal bacteria, and dietary biotin deficiency is almost absent.

Regarding the use of biotin, it has been approved in Japan as a Japanese Pharmacopoeia standard, a cosmetic raw material standard and a feed additive. However, since it is not approved as a food additive, it cannot be used for food and drink at present. On the other hand, with the westernization of eating habits in recent years, the number of obese and diabetic patients due to excessive calorie intake is increasing,
It is known that the serum biotin concentration in these patients is significantly reduced.

[0006] Regarding the physiological action of biotin, it is involved in various carbonic acid fixation, carbonic acid transfer, decarboxylation reaction, etc. as a coenzyme, and it regulates metabolism of sugar system, rate-controlled stepwise control of fatty acid synthesis system, amino acid and nucleic acid. It is known to be involved in the metabolic system of. The decrease in these metabolic activities causes acidosis due to the accumulation of organic acids in the body, which causes a decrease in the cellular immune system. Therefore, since biotin plays an important role in the metabolism of carbohydrates and lipids, it is considered that biotin-containing foods can maintain nutritional balance in modern diet and be useful foods from the viewpoint of adult disease prevention. To be It is expected that biotin will be approved as a food additive as part of measures to prevent obesity and adult diseases.

[0007] A known technique utilizing the physiological action of biotin is a "fatigue-improving agent" containing biotin, which acts on the lactate metabolism pathway and the oxaloacetate supply pathway, as an active ingredient. 6-305963
No.), a "diabetic therapeutic agent" in which biotin is administered alone or together with insulin (JP-A-57-99520), and a "medicine and dietary composition" relating to the treatment of inflammatory disorders including rheumatoid arthritis (special feature). Kai 58-1
No. 64510).

[0008]

DISCLOSURE OF THE INVENTION The present inventors have focused on the relationship with the production of biotin by intestinal flora when developing foods and drinks that effectively utilize the physiological action of biotin. That is, the inventors have found that the concentration of biotin in blood can be increased by ingesting oligosaccharides to adjust the intestinal flora environment and simultaneously ingesting biotin, and completed the present invention.

[0009] An object of the present invention is to improve the tendency of intake of biotin to be insufficient by frequently using processed foods and drinks in daily eating habits. The object is to provide a method for increasing the concentration of biotin in the medium. Another object of the present invention is to provide an oligosaccharide- and biotin-containing food or drink that has the effect of increasing the concentration of biotin in blood.

[0010]

According to the present invention, there is provided a method for increasing the concentration of biotin in blood, which comprises simultaneously ingesting an oligosaccharide and biotin. Further, according to the present invention, there is provided a biotin-containing food or drink containing an oligosaccharide and biotin, which increases the biotin concentration in blood.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION Embodiments of the present invention will be described in detail below. The biotin-containing food or drink of the present invention that increases the blood biotin concentration is characterized by containing oligosaccharides and biotin.

The amount of biotin that can be contained in the biotin-containing food and drink of the present invention is 30 to 400 μg / day, estimated from the recommended daily intake of biotin in other countries. Is preferably used. It is preferably 30 to 300 μg / day.

However, even if biotin is excessively ingested, it is excreted in the urine without accumulating in the body, so 400 μg /
There is no problem if taken more than a day. This means that 60 mg / day of biotin was orally administered to humans for 3 weeks, and 100 mg / day.
It has been reported that no side effects are observed when administered intramuscularly for 3 weeks / day (Food Protecti
on Committee, National Res
search Council: Survey of
Substances Generally Recon
gnized as Safe. Biotin. N
national Academy of Science
es. Washington, D.R. C. 53 pp
(1972)).

The biotin used in the present invention includes foods containing a large amount of biotin, such as those obtained from natural products such as cereals, soybeans, livestock offal, egg yolk, nuts, royal jelly, yeasts and other microbial cells, and pharmaceuticals. Any of the chemically synthesized products mainly used in. Each can be appropriately selected depending on the processed form of food and drink and the application.

The amount of the oligosaccharide that can be contained in the biotin-containing food or drink of the present invention is not less than the minimum effective amount that exhibits an intestinal regulating action such as intestinal bifidobacteria growth activity. The effective daily intake (per 60 kg body weight) of the effective oligosaccharide contained in each oligosaccharide is 3 in the case of soybean oligosaccharide.
g, fructooligosaccharide 5g, isomaltooligosaccharide 1
3.5 g, milk fruit oligosaccharide 2 g, xylooligosaccharide 1.
It is set to 5g. In addition to various other oligosaccharides, one or more kinds of these oligosaccharides may be used in combination. The minimum effective oligosaccharide amount in the case of combination use is the additive amount of the calculated values according to the compounding ratio of the minimum effective oligosaccharide amount of each oligosaccharide.

The preferable intake amount of each oligosaccharide varies depending on the kind of oligosaccharide, but from the minimum effective intake amount of each or more to the maximum ineffective amount (maximum possible without causing abdominal symptoms such as diarrhea, flatulence, glistening, and flatus) Intake). For example, 0.05 to 0.8 for soybean oligosaccharides
5 / kg body weight / day, fructooligosaccharide 0.08-0.
3 / kg body weight / day, isomaltooligosaccharide 0.23 ~
0.6 / kg body weight / day.

Although the oligosaccharides and biotin may be simultaneously ingested in effective amounts within the same day, it is desirable to ingest them continuously every day. By ingesting continuously every day, the healthy state of the intestinal flora environment due to oligosaccharides is maintained,
Absorption of biotin into blood has a synergistic effect. In order to increase the blood biotin concentration by simultaneously ingesting the oligosaccharide of the present invention and biotin, it is preferable to continuously ingest food and drink containing an effective amount of oligosaccharide and biotin at the same time every day.

The period of ingestion of the oligosaccharide and biotin of the present invention is
Although affected by individual differences and dietary content, etc., in general, ingesting an effective amount for two or more consecutive days produces an effect of increasing blood biotin concentration compared to ingestion of biotin alone. This effect disappears when the simultaneous ingestion of oligosaccharide and biotin is stopped, that is, when only biotin is independently ingested.

As the composition components other than biotin and oligosaccharide which can be contained in the biotin-containing food and drink containing oligosaccharide and biotin of the present invention, all general food components other than raw egg white containing active avidin are used. be able to. Specific examples include organic acids and organic acid salts, monosaccharides, polysaccharides, lipids, proteins / amino acids, thickeners, fragrances, and pigments.

The biotin-containing food and drink of the present invention may be in any form of product, for example, beverage, solid form,
Examples thereof include gel and powder.

In addition, the biotin-containing food and drink of the present invention contains oligosaccharides and biotin, for example, processed milk products, processed livestock products, processed marine products, processed agricultural products such as cereals, beans and fruits, seasoned foods, and cooked foods. It may be contained in foods, confectioneries, desserts, health foods, soft drinks, fruit juice drinks, flavored drinks, milk drinks, alcoholic drinks, health drinks and the like.

[0022]

The present invention will be described below with reference to examples.
The present invention is not limited to these examples.

Example 1 The following materials were mixed and a biotin-containing beverage containing biotin and oligosaccharide was prepared to prepare a test solution A.

(1) Compounding amount of test liquid A (in 100 ml) ────────────────────────── Biotin * 300 μg Soybean oligosaccharide ** 18.8g Citric acid 0.29g Sodium citrate 0.13g Perfume 0.20g Purified water 86.0g ────────────────────────── * : Trade name "Roche" biotin (manufactured by Nippon Roche) **: R. Brix76, effective oligosaccharide (stachyose, raffinose, etc.) content 22.0 g / 100 g

(2) Formulation of Test Liquid B A test liquid B was prepared by separately adjusting the soybean oligosaccharide in the above test liquid A blended with glucose fructose syrup (manufactured by Japan Food Processing Co., Ltd.).

(3) Preparation of Test Liquids A and B 1316 g of soybean oligosaccharide (trade name: soybean oligosaccharide syrup, manufactured by Calpis Food Industry Co., Ltd.) was dissolved in 5166 g of purified water with stirring, and then 203 g of 10% by weight citric acid solution. And 10% by weight sodium citrate solution 91 g, 0.01
210 g of a wt% aqueous biotin solution and 14 g of a fruit juice flavor were added and dissolved by stirring. 95% of the obtained mixed solution of 7000 g
Sterilization was carried out at a temperature of up to 0 ° C., 100 g of each was hot-packed in 100 ml bottles, and then water-cooled to obtain a test solution A. Test liquid B was also prepared by the same treatment.

Ten healthy adults (23 to 43 years old) were divided into an oligosaccharide-administered group and a control group (oligosaccharide-non-administered group), 5 persons each. In the oligosaccharide-administered group, 1 week of soybean oligosaccharide beverage (trade name: Oligo CC, Calpis Food Industry Co., Ltd., stachyose, containing 3 g of raffinose, no biotin added) was administered 2 weeks before administration of the test solution A. Book / drank for 1 day.
At the start of the test, the test solution A was switched to and consumed. The control group did not drink the soybean oligosaccharide drink from 2 weeks ago, but allowed the test solution B to be drunk at the start of the test.

After drinking the test solution, 0, 0.5, 1, 2,
Blood was collected 4, 8, 24, 48 and 72 hours later, respectively.
The collected blood was centrifuged to obtain serum, and the amount of biotin was measured with a biotin detection kit (manufactured by γ-Biopharm). The result is shown in FIG. Serum biotin levels in the oligosaccharide-administered group were significantly higher than those in the control group, and the oligosaccharide administration effect was also significantly recognized in the test results by the two-way analysis of variance analysis.

Example 2 Using the test solutions A and B prepared in Example 1,
The continuous intake test described below was performed. Fifteen healthy adults (20 to 38 years old) were divided into three groups of five, each of which was designated as test liquid A, test liquid B, and soybean oligosaccharide beverage group. Each group was allowed to drink the test solution once a day for two consecutive weeks. Blood was collected before drinking, during the drinking period, and after drinking, and the biotin concentration in the blood was measured. Blood was collected every 48 hours during the drinking period.
The result is shown in FIG. It was confirmed that the test liquid A (oligosaccharide / biotin) administration group can maintain the blood biotin concentration at a significantly higher level than the control group. No increase in blood biotin concentration was observed in the soybean oligosaccharide drink group.

Example 3 Fruit jelly was produced by the following formulation. Each blended material was added with stirring and uniformly dissolved. The pectin was separately dissolved in water and used.
The solution was sterilized by reaching 85 ° C., and then hot-packed in a cup (made of polystyrene) having a capacity of 100 ml, and cooled by standing to obtain a fruit jelly product.

(1) Blended amount of fruit jelly products (in 100 g) ────────────────────────── Soybean oligosaccharide 15.0 g Glucose fructose Liquid sugar 3.0 g 6 times concentrated Valencia orange juice 1.0 g Pectin 0.65 g Crystalline citric acid 0.60 g Biotin 300 μg Purified water 79.45 g ──────────────────── ─────── A fruit jelly product was subjected to a continuous ingestion test for one person, one day, and one cup for two weeks under the same test settings as in Example 2. As a result, almost the same result as Example 2 was confirmed. Was done.

Example 4 A tablet confectionery was produced with the following formulation. V for each ingredient
The mixture was uniformly mixed using a mold powder mixer. The obtained mixed powder is tableted by a tableting machine (manufactured by Hata Tekko Co., Ltd., model HT-P18A, tableting pressure 500 kg / cm ² ) to give a tablet-type tablet confectionery product (about 1.5 g). / 1 tablet) was obtained.

(1) Blending amount of tablet confectionery products (per tablet) ────────────────────────── Soybean oligosaccharide powder * 1 .4 g Crystalline citric acid 0.04 g Vanilla flavor 0.04 Biotin 43 μg Enzymatic saccharified starch syrup 0.02 g Lubricant 0.02 g ──────────────────────── ─── *: Effective oligosaccharides (stachyose, raffinose, etc.) content 31.6 g / 100 g

The tablet confectionery product was subjected to a continuous ingestion test of 7 tablets per person per day for 2 weeks under the same test settings as in Example 2. As a result, almost the same results as in Example 2 were confirmed.

[0035]

As described above, according to the present invention, processed foods having a significantly reduced biotin content in the processing process in modern dietary life are often used, and biotin intake tends to be insufficient. Blood biotin concentration can be increased by ingesting biotin simultaneously with the oligosaccharide.

Further, according to the present invention, the biotin concentration in blood can be increased by ingesting biotin at the same time as the oligosaccharide, and biotin deficiency symptoms such as obesity, diabetic patients and skin disease patients can be improved.

[Brief description of drawings]

FIG. 1 is a graph showing a change with time in the amount of serum biotin in Example 1.

FIG. 2 is a graph showing the change with time of the amount of serum biotin in Example 2.

Continuation of the front page (51) Int.Cl. ⁶ Identification number Reference number within the agency FI Technical indication location A61K 47/26 A23L 2/00 F (72) Inventor Akihiro Masuyama 5-11-10 Fuchinobe, Sagamihara-shi, Kanagawa Calpis R & D Center of Food Industry Co., Ltd.

Claims (2)

[Claims] 1. A method for increasing blood biotin concentration, which comprises simultaneously ingesting oligosaccharide and biotin. 2. A biotin-containing food or drink which increases the blood biotin concentration, which comprises an oligosaccharide and biotin.