JPH09215675A - Bag for 13c exhalation inspection - Google Patents

Abstract

PROBLEM TO BE SOLVED: To accurately measure<13> C concentration in vivo by providing an exhalation resistance means as resistance to the blowing of exhalation into a mouth part of an exhalation bag to prevent outside air inhaled deeply from being discharged directly. SOLUTION: An exhalation bag is a self-sustaining bag 2 comprising a resin film and a laminated sheet of aluminum foils. In the exhalation bag, a rigid tubular mouth part 3 is mounted sealed on a mouth part and a cap 4 is removably mounted. An exhalation resistance means 5 is fitted in the tubular mouth part 3 of the exhalation bag to generate a specified blowing resistance during the blowing of exhalation. As the resistance to blowing during the blowing of the exhalation develops a double breathing, even when a subject takes a deep breathing, outside air sucked is mixed sufficiently with air containing<13> C in vivo thereby eliminating drop in the<13> C in vivo. In other words, this prevents direct discharge of fresh outside air sucked by the deep breathing thereby achieving a mixing with air containing<13> C in vivo before the exhaustion.

Description

Detailed Description of the Invention

[0001]

This invention relates to stable isotopes of carbon ¹³
The present invention relates to an exhalation bag for efficiently exhaling exhaled air from a subject administered with a compound labeled with C.

[0002]

2. Description of the Related Art Conventionally, by administering a compound labeled with a stable carbon isotope ¹³ C and monitoring ¹³ C appearing in exhaled breath over time, digestion, absorption and metabolism of the compound in the body can be investigated. Methods for measuring are known, digestion and absorption tests,
It is used as a means for testing internal bacteria, testing liver function, and the like.

Such measurement of ¹³ C in exhaled breath is carried out, for example, in the human stomach by inhabiting and inhabiting the stomach, and is associated with the pathogenesis of chronic active gastritis, duodenal ulcer and the like.
It is used for the examination i) and liver function test of infants with atopic dermatitis.

The present inventors previously labeled ¹³ C by using an exhalation bag consisting of a laminated sheet of a plastic film and aluminum foil, and a self-supporting bag having a rigid tubular mouth attached to the mouth. The simple and reliable collection, storage, and transfer of exhaled breath from a subject administered with the compound described above, and the measurement of ¹³ C exhaled breath even when the exhaled breath collection place and the analytical measurement place are separated temporally and spatially. We proposed a ¹³ C exhalation measurement system that enables efficient measurement.

However, when the subject exhales into the exhalation bag, first, he / she takes a deep breath and then exhales into the exhalation bag, so that the first half of the exhalation becomes the outside air that does not contain ¹³ C taken by the deep breath. After that, exhaled breath including ¹³ C in the body comes in. Therefore, breath taken in breath bag is made as the concentration of ¹³ C were reduced, it was found that a problem that it is not possible to accurately measure the abundance of ¹³ C in the subject is exist.

[0006]

DISCLOSURE OF THE INVENTION The present invention prevents deeply breathed outside air from being directly discharged when a subject administered with a compound labeled with ¹³ C blows into the exhalation bag. It is intended to provide an exhalation bag capable of accurately measuring the ¹³ C concentration of.

[0007]

[Means for Solving the Problems] The means adopted by the present invention to solve the above-mentioned problems are to administer a compound labeled with a stable carbon isotope ¹³ C to a subject for measuring ¹³ C in exhaled breath. The expiratory bag for collecting the exhaled breath, characterized in that expiratory resistance means for resisting blowing of the exhaled breath is provided in the mouth of the expiratory bag.

Further, the expiratory resistance means is a filter means.

Further, the expiratory resistance means is characterized by comprising a tubular member having a filter means attachable to the mouth of the expiratory bag.

Further, the expiratory resistance means is characterized by comprising a narrow passage which can restrict the flow of air.

Further, the exhalation bag is characterized by comprising a self-supporting bag made of a laminated sheet of a plastic film and aluminum foil.

Further, the exhalation bag is composed of a laminated sheet of polyester film, aluminum foil, stretched nylon, and polyethylene film, and the folded bottom material is fused in an arc shape to close the opened bottom and close the mouth. It is characterized by being a self-supporting bag body to which a rigid tubular mouth portion is attached and a cap is detachable.

Furthermore, an elastic sealing body is provided on the cap mounted on the mouth of the exhalation bag so that the injection needle can be pierced from the outside.

[0014]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of the present invention will be described in detail below. In the figure, (1) is an exhalation bag according to the present invention, which does not need to be exchanged with outside air, has a high sealing property, allows a subject to easily exhale, and is easily connected to an analyzer. When it is not in use, it is folded and kept in a flat form, the volume can be easily expanded when exhaling, and there is no local deformation when crushing for exhalation, and exhalation can be easily pushed out. It is preferable that it is possible.

An example of such an exhalation bag (1) is a self-supporting bag (2) made of a laminated sheet of plastic film and aluminum foil. A rigid tubular mouth portion (3) is hermetically attached to the mouth portion of the exhalation bag (1), and a cap (4) is detachably attached. The laminated sheet constituting the bag body (2) is, for example, a polyester film, an aluminum foil, a stretched nylon film or a polyethylene film.
It is composed of a laminated sheet of layers, and is formed into a bag body having an opened lower surface, which is formed by joining two laminated sheets on the upper side and both side pieces by heat welding or adhesion and sealing. The opened lower surface is sealed by joining the folded bottom sheet in an arc shape. When the upper side of the bag body (2) is joined, the tubular mouth portion (3) is simultaneously attached and tightly joined.

The exhalation bag (1) is flatly folded when not in use, and when exhaled, the bottom portion of the exhalation bag (1) expands to become a self-supporting state. The expandability and self-supporting property of the bag is that the bag can be easily expanded when breath is inhaled to store a required amount of breath, and when the cap is removed, the bag retains its shape. There is no danger that the exhaled breath will be pushed out, and there is an advantage that it can be pushed out when it is crushed. The volume of the exhalation bag is appropriately set according to the exhalation amount used for one exhalation analysis, but since the usual required exhalation amount is 50 ml, it is desirable to have a volume of 50 ml or more.

In the tubular mouth portion (3) of the exhalation bag (1), exhalation resistance means (5) for producing a predetermined blowing resistance at the time of exhalation is provided. Since breathing resistance causes abdominal breathing during inhalation, even if the subject takes a deep breath, the inhaled outside air is sufficiently mixed with the air containing ¹³ C, and the ¹³ C concentration may decrease. Disappear. That is, the fresh air sucked by deep breathing is prevented from being directly discharged, and is mixed with the air containing ¹³ C in the body before exhausting. Thus, exhaled air that accurately represents the ¹³ C concentration of the subject is collected in the exhaled bag, and accurate ¹³ C concentration measurement can be realized.

Expiratory resistance means arranged in the tubular mouth (3)
In (5), in the illustrated embodiment, a filter means such as an ultrafiltration membrane, a membrane or a filter paper, which acts as an air flow resistance, is used. However, it is needless to say that it is not limited to such a filter means, and may be a thin slit or hole, a valve means, or the like. In essence, it can be anything that provides resistance that requires abdominal breathing when the subject exhales. In the illustrated form, the exhalation resistance means (5) is provided with a tubular member (8) which can be inserted into the tubular mouth portion (3), and the filter means (6) is attached to the tubular member (8). There is.

The cap (4) attached to the tubular mouth has a
An elastic sealing body (7) capable of piercing the injection needle (6) is attached, and the injection needle (6) can be pierced and the exhaled air in the exhalation bag can be taken out for analysis without removing the cap. You can do it. The elastic seal (7) allows the needle to be pierced, and when the needle is removed,
It is preferable to have rubber elasticity so that the puncture hole of the injection needle is elastically closed. As such an elastic sealing body having rubber elasticity, for example, silicon, polytetrafluoroethylene or the like can be used. By using the cap equipped with the elastic sealing body, it is not necessary to remove the cap at the time of analysis, and the handling becomes convenient.

The breath bag in which a breath resistance means were taken of a subject breath in the mouth of the invention was measured for the concentration of ¹³ C, ¹³ C in the breath collected at breath bag no expiratory resistance means About ¹³ times higher concentration of ¹³ C was measured, and this ¹³ C concentration was almost the same as the concentration of ¹³ C directly measured in the body of the subject. Further, when the residual amount of expired air in the expired bag in which the elastic sealing body (7) was attached to the cap (4) was measured with time, there was almost no change in the residual amount even after storage for 3 months.

In FIG. 4, after the amino group labeled with ¹³ C was drunk, 30 minutes later, exhaled air was continuously collected 7 times, and the amount of carbon dioxide gas labeled with ¹³ C was measured. In FIG. 4, A
Collects exhaled air with a conventional exhalation bag, and B discards one-third of the initial exhaled breath and leaves the remaining two-thirds without breathing.
Exhaled air was collected. CG are the samples of exhaled air using the exhalation bag of the present invention. In the exhalation bag of C,
Filter paper No. No. 1 for the exhalation bag D. 2 were used respectively. The E breath bag has a 0.22 micron pore size Millipore filter, the F breath bag has a 0.45 micron pore size filter, and the G breath bag has a 0.02 micron pore size filter. Were used as expiratory resistance means. As a result of the measurement, the exhaled air collected by the exhalation bag equipped with the expiratory resistance means of the present invention is almost exactly 13C.
Since the amount of carbon dioxide gas is included, the effect of the exhalation bag of the present invention could be confirmed.

[0022]

According to the present invention, the exhaled breath of a subject can be collected without lowering the ¹³ C concentration in the exhaled breath, and the attachment / detachment of the cap is unnecessary during the analysis, thus improving the workability.

[Brief description of drawings]

FIG. 1 is an external perspective view of an exhalation bag according to the present invention.

FIG. 2 is a cross-sectional view of the main part.

FIG. 3 is a perspective view showing a piercing state of an injection needle.

FIG. 4 is a graph showing the results of an experiment confirming the effects of the present invention.

[Explanation of symbols]

 (1) Exhalation bag (2) Bag (3) Tubular mouth (4) Cap (5) Exhalation resistance means (6) Injection needle (7) Elastic seal (8) Cylindrical object

Claims (7)

[Claims] 1. An exhalation bag for collecting exhaled air from a subject to measure ¹³ C in exhaled air by administering a compound labeled with a stable isotope ¹³ C of carbon. A ¹³ C breath test bag characterized by being provided with an expiratory resistance means which is resistant to blowing. 2. The ¹³ C breath test bag according to claim 1, wherein the breath resistance means is a filter means. 3. The ¹³ C breath test bag according to claim 2, wherein the expiratory resistance means comprises a tubular member having a filter means that is attachable to the mouth of the expiratory bag. 4. The ¹³ C according to claim 1, wherein the expiratory resistance means comprises a narrow passage capable of restricting the flow of air.
Breath test bag. 5. The ¹³ C according to claim 1, wherein the exhalation bag is a self-supporting bag body made of a laminated sheet of a plastic film and aluminum foil.
Breath test bag. 6. An exhalation bag is made of a laminated sheet of polyester film, aluminum foil, stretched nylon and polyethylene film, the folded bottom material is fused in an arc shape, the opened bottom is closed, and the mouth is opened. The ¹³ C breath test bag according to any one of claims 1 to 5, wherein the bag comprises a self-supporting bag body having a rigid tubular mouth portion attached thereto and a cap detachably attached thereto. 7. The elastic sealer capable of piercing an injection needle from the outside is disposed on a cap attached to the mouth portion of the exhalation bag, according to any one of claims 1 to 6. ¹³ C breath test bag.