JPH0920606A - Degerming cleaning agent for microtiter tray for medical examination - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a cleaning agent having a strong degerming cleaning function and suitable foaming ability, capable of easily decomposing and removing a body fluid and at the same time surely killing microbes and viruses by compounding hypochlorite with specific 5 kinds of supplementary agents. SOLUTION: This cleaning agent is obtained by compounding (a) an alkyldiphenyletherdisulfonic acid salt, (b) an anionic surfactant consisting of an alkylphenylethersulfonate salt, (c) a hypochlorite, (d) a polyacrylic acid salt (a metal chelating agent), (e) a silicate (a metal corrosion inhibitor) and (f) an alkali hydroxide (a pH adjusting agent). The components (a)+(b) are preferably added in an amount of 1-20wt.% based on the component (c), and the weight ratio (a)/(b) is preferably 5-20. The components (d) and (e) are added in the amounts of 1-10wt.% and 1-20wt.% based on the component (c), respectively. The component (a) is preferably a compound of formula I (R1 is a 12-14C alkyl), and the component (b) is preferably the compound of formula II [R2 is a 8-18C alkyl; (n)=2-5]. Further, the component (f) is preferably added in a range capable of adjusting the pH of the cleaning agent to 11-13.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a microplate disinfecting detergent, which is generally used for a body fluid test such as a blood test and a serum test in a medical institution such as a hospital or a medical research institution. The present invention relates to a disinfecting detergent suitable for cleaning.

[0002]

2. Description of the Related Art Generally, as shown in FIG. 1, a microplate for medical examination has wells 2 which are a large number of circular recesses arranged vertically and horizontally on one side of a rectangular plate 1 made of synthetic resin or glass. These wells 2 are each provided with a body fluid such as blood or serum of a subject for one person in each row, and a required test reagent is dropped to perform blood type test, HB test, and HB test.
Various tests such as an antigen test and an HIV (AIDS virus) test are conducted, and after the test, the stains including the body fluid and the reagent adhering thereto are removed and then repeatedly used. However, stains at the time of inspection include bacteria and viruses in body fluids, and if these stains remain, they will naturally affect the next inspection and cause misdiagnosis, etc. Therefore, it is necessary to completely remove dirt and sterilize it in the above cleaning.

Conventionally, a neutral or alkaline detergent containing an anionic or nonionic surfactant has been used for washing the microplate 1. However, removal of protein-based stains in body fluids is very difficult. In addition to being difficult, sufficient sterilization properties cannot be obtained, and air bubbles remain in the wells 2 and easily cause poor cleaning. Especially, in the fully-automatic microplate washer often used in recent years Poor cleaning due to is a serious problem.

The fully automatic microplate washing machine is shown in FIG.
As shown in FIG. 3, dozens of microplates 1 ... are erected in the cleaning tank 11 of the apparatus main body 10 in the upright state by utilizing the fitting groove portions 12 at the bottom of the tank, and the mutual intervals are 15 to 20 mm.
First, the nozzle jet method is used to rinse with tap water, the water is drained by blowing air, and then the cleaning liquid 3 diluted in a chemical liquid tank (not shown) is gradually introduced into the cleaning tank 11, and the microplate 1 To the upper part of the washing liquid 4 for a certain period of time to sterilize and wash,
After discharging the cleaning liquid 3 in the cleaning tank 11, the nozzle jet method is used to rinse with tap water to wash away the cleaning liquid, and further a final rinse is carried out with distilled water (demineralized water), and finally it is dried by blowing air to finish. Has been done. In addition, 13 in FIG. 2 is an opening / closing lid of the cleaning tank 11, and 14
Is a viewing window provided on the opening / closing lid 13, and 15 is an operation panel.

However, since the microplate 1 at the time of sterilization washing is immersed in the washing liquid 4 in an upright state, the wells 2 ...
The bubbles that have entered are difficult to escape, and as shown in FIG. 4, bubbles 4 remain in the upper corner portion of the well 2 and the dirt on the surface in contact with the bubbles 4 is not removed.

In the case of manual cleaning, the microplate can be carefully rubbed in the cleaning solution to prevent the stain from remaining. However, as described above, the cleaning power and the disinfecting property of the conventional cleaning solution are reduced. Since it is not enough, it takes a lot of time to completely remove the dirt, and since it is a manual work, it is unavoidable that the cleaning degree varies. There was a problem of being scarce.

[0007]

In view of the above situation, the present invention provides a microplate disinfecting detergent for medical examinations,
It has a strong sterilizing and detergency and an appropriate foaming property, and can easily decompose and remove body fluid components such as proteins and fats, can surely kill bacteria and viruses, and wet and stain the plate surface. It has a high osmotic power to cells, can reliably remove air bubbles in wells, does not cause cleaning failure even when applied to cleaning with a fully automatic microplate washer, and has excellent storage stability. Is intended.

In order to achieve the above object, the microplate disinfecting detergent for medical examination according to claim 1 of the present invention comprises:
As essential components, a) alkyl diphenyl ether disulfonate, b) alkyl phenyl ether sulfate ester salt, c) hypochlorite, d) polyacrylate, e)
The structure is composed of an aqueous solution containing silicate and f) an alkali hydroxide.

According to a second aspect of the present invention, in the microplate disinfecting detergent for medical examination according to the first aspect, a component of an alkyldiphenyl ether disulfonate and a component of b is added to a component c of hypochlorite. The total amount of the alkyl phenyl ether sulfate ester salt is mixed in the range of 1 to 20% by weight.

A third aspect of the present invention is the above-mentioned claim 1 or 2.
Of the microplate disinfectant cleaning agent for medical examination of
Ingredients alkyl diphenyl ether disulfonate and b
The composition is such that the compounding ratio of the component alkyl phenyl ether sulfate ester salt is 5 to 20 in terms of a / b weight ratio.

According to a fourth aspect of the present invention, in the microplate disinfecting detergent for medical examination according to any one of the first to third aspects, the hypochlorite of the c component is replaced with the polyacrylate of the d component. Is adopted in the range of 1 to 10% by weight.

According to a fifth aspect of the present invention, in the microplate disinfecting detergent for medical examination according to any one of the first to fourth aspects, the component e is a silicate of 1 component with respect to the component c of hypochlorite. The composition is used in the range of up to 20% by weight.

According to a sixth aspect of the present invention, in the microplate disinfecting detergent for medical examination according to any one of the first to fifth aspects, the alkyl diphenyl ether disulfonate as the component a is of the following general formula (I);

[0014] The configuration is composed of the following.

In a seventh aspect of the present invention, the alkylphenyl ether sulfate ester salt of the component b in the microplate disinfecting detergent for medical examination according to any one of the first to sixth aspects is represented by the following general formula (II);

[0016] The configuration is composed of the following.

According to claim 8 of the present invention, the alkali hydroxide of the component f in the microplate disinfecting detergent for medical examination according to any one of claims 1 to 6 has a pH of 11 to 1 as a detergent.
The composition adopted is mixed in the range of 3.

[0018]

Detailed structure and action of the invention Among the essential components of the microplate disinfecting detergent for medical examination of the present invention, the hypochlorite of the component c has a strong oxidizing power (effective chlorine content in a commercially available aqueous solution is 12%). In addition to exhibiting a high bactericidal action based on this oxidizing power, it also has a cleaning action in the alkaline region, and a strong decomposition and removal action against protein-based stains that are generally insufficient to wash with alkaline detergents. Indicates. Sodium hypochlorite is suitable as such a hypochlorite. In addition, the compounding amount in the disinfectant detergent is from a to
It is preferable that the total amount of the active ingredient of f is 100% by weight, and it is about 10 to 50% by weight. If it is too small, the above-mentioned action cannot be sufficiently exhibited, and if it is too large, the action of other compounding ingredients is relatively large. Insufficient due to a decrease in the compounding ratio.

The alkyl diphenyl ether disulfonate of the component a and the alkyl phenyl ether sulfate ester of the component b are both anionic surfactants, but due to the synergistic effect of the combination of these two specific anionic surfactants, The appropriate foamability and cleaning power required for cleaning can be obtained, and excellent wettability to the microplate surface,
In addition, a high penetrating force against dirt adhering to the plate is exhibited, which makes it very easy to separate the bubbles from the wells, and the bubbles remain in the wells even when applied to the fully automatic microplate washing machine described above. The cleaning failure due to is surely prevented. Moreover, since the hypochlorite of the component c has a strong oxidizing power, it becomes unstable when mixed with a general surfactant and gradually decomposes to reduce the effective chlorine concentration. The anionic surfactants of the a-component and the b-component have a good affinity with hypochlorite, and therefore have an advantage of not impairing the stability of hypochlorite. Therefore,
The microplate disinfecting detergent for medical examinations of the present invention is excellent in storage stability and maintains a strong disinfecting detergent power for a long period of time.

The compounding amount of the alkyl diphenyl ether disulfonate of the component a and the alkyl phenyl ether sulfate ester of the component b is 1 to 20% by weight based on the total amount of the components and the hypochlorite of the component c. If the amount is too small, the cleaning power becomes insufficient, and conversely if the amount is too large, the foaming property of the disinfecting detergent becomes high, and the bubbles of the detergent tend to remain in the wells of the microplate. Become. Further, the mixing ratio of these a component and b component is 5 in terms of a / b weight ratio.
It is preferable to set it in the range of -20, and outside this range, there is a problem that the compatibility with hypochlorite deteriorates and precipitation of insoluble matter occurs. Particularly, when the amount of the component a is too small, the washing permeability for the body fluid stains adhering to the wells of the microplate becomes insufficient, and when the ratio of the component b becomes high, the foaming property becomes excessive, leading to poor washing, which is preferable. Absent.

The alkyl diphenyl ether disulfonate as the component a is not particularly limited, but sodium alkyl diphenyl ether disulfonate represented by the following general formula (I) is preferable.

[0022]

On the other hand, the alkyl phenyl ether sulfate ester salt of the component b is not particularly limited, but sodium alkyl phenyl ether sulfate represented by the following general formula (II) is preferable.

[0024]

Incidentally, the carbon number of R _{1 in} the sodium alkyldiphenyl ether disulfonate of the general formula (I) is out of the range of 12 to 14, and R _{2 in the} sodium alkylphenyl ether sulfate of the general formula (II). If the number of carbon atoms is outside the range of 8 to 18, the activity will be weak, and the cleaning effect when using these will be reduced.

As the metal chelating agent, the polyacrylic acid salt of the component d has a function of dissolving metal components such as calcium in the body fluid adhering to the wells of the microplate, and also acts as a cleaning aid. It is a thing. The polyacrylic acid salt is not particularly limited, but sodium polyacrylate is preferable, and those having an average molecular weight of about 6,000 to 10,000 are particularly preferable from the viewpoint of stability of the detergent and suppression of reduction of available chlorine. However, the amount of the polyacrylic acid salt compounded is preferably in the range of 1 to 10% by weight with respect to the hypochlorite of the component c. If it is too small, the sufficient effect cannot be exhibited, and if it is too large. However, it is uneconomical because it cannot be expected to have a greater effect.

As metal chelating agents other than polyacrylic acid salts, polyphosphoric acid salts, EDTA (ethylenediaminetetraacetic acid) salts, NTA (nitrilotriacetic acid) salts, etc. can be considered, but polyphosphoric acid salts are washed without phosphorus. It cannot be used as an agent, and EDTA salts and NTA salts have a problem that the available chlorine of hypochlorite is reduced.

On the other hand, the hypochlorite of the component c is corrosive to various metals in an aqueous solution, and if it is left as it is, it may corrode or corrode or discolor the metal washing tank or piping. In the disinfecting detergent of the invention, the silicate as the component e has a function of preventing metal corrosion due to hypochlorite. As such a silicate, sodium silicate and potassium silicate are preferable, but the compounding amount thereof is in the range of 1 to 20% by weight based on the hypochlorite of the component c from the viewpoint of the effect and solubility. Is good.

The alkali hydroxide of the component f is a PH which prevents the effective chlorine decomposition of the hypochlorite of the component c due to a decrease in PH.
It has a function as a regulator and a function for improving the cleaning property against stains of fats and lipids, and sodium hydroxide and potassium hydroxide are preferable. The amount of this alkali hydroxide blended is 11 to PH of the disinfecting detergent (product form).
The amount required to achieve a range of 13 is suitable.

The microplate disinfectant cleaning agent for medical examination of the present invention is an aqueous solution prepared by dissolving the above-mentioned components a to f in water and generally has a concentration of the hypochlorite of the component c of 4 in the aqueous solution.
It is commercialized in the form of an undiluted solution of about 0 to 60% by weight, and is set to be diluted with water by about 30 to 40 times for washing the microplate. In addition to the above essential components a to f, various additives such as citric acid as a chelating aid and an organic acid such as DL-malic acid may be added to the disinfectant detergent, if necessary. You may mix suitably.

This microplate disinfectant detergent for medical examinations has a strong disinfectant detergent power and an appropriate foaming property, and can easily decompose and remove body fluid components such as proteins and fats, and bacteria. You can definitely kill the virus
Moreover, since it has high wettability to the plate surface and penetrating power to stains, it can uniformly exert a strong disinfecting cleaning power on the entire plate without leaving air bubbles in the well, so it can be used for cleaning with a fully automatic microplate cleaning machine. Even if it is possible to reliably prevent cleaning failure due to residual air bubbles in the well,
It is characterized by good storage stability, little deterioration in sterilizing and detergency over time, and little corrosiveness with respect to metal, so that it is less likely to invade metal parts such as a washing tank and piping used for washing microplates.

[0032]

EXAMPLE Anionic surfactants (a) and (b), sodium hypochlorite, sodium hydroxide, sodium polyacrylate, sodium silicate, and potassium hydroxide were added in the proportions shown in Table 1 below. No. 1 to 9 germicidal cleaners were prepared. The blending amount of each component in Table 1 is parts by weight, and the total amount of the sterilizing detergent containing water added to these components is 100 parts by weight, and in all cases, PH is in the range of 11 to 13.
As sodium hypochlorite, a commercially available aqueous solution having an effective chlorine amount of 12% (concentration of 50%) is used, and as sodium polyacrylate, trade name Aron A-20U manufactured by Toagosei Co., Ltd.
(Average molecular weight of 7,000) was used. The anionic surfactants (a) and (b) are as follows.

Anionic surfactant (a): sodium alkyldiphenyl ether disulfonate in which R ₁ in the general formula (I) is an alkyl group having 9 carbon atoms (nonyl group). Anionic surfactant (b); R ₂ in the general formula (II) is an alkyl group having 9 carbon atoms (nonyl group), and n is 3 to 9
Alkyl phenyl ether sodium sulfate, which is an integer of.

[0034]

[Table 1]

Each of the above sterilizing detergents was tested for washability, storage stability and corrosiveness to metal when applied to washing with a fully automatic microplate washer. The results are shown in Table 2 below. The test method for each item is as follows.

[Disinfection cleaning method] 40 acrylic resin microplates (120 used for blood and serum tests)
Hole type ... 130 × 80 × 12 mm, hole diameter 6 mm) is set in a stainless steel washing tank of a fully automatic microplate washing machine (made by Techno-Eight Co., Ltd.), and rinsed with tap water by nozzle / jet method by timer setting ( Flow rate about 18
Liter / min) for 5 minutes, and drained by blowing air for 2 minutes.
65 liters of the double diluted cleaning solution was gradually introduced from the chemical solution tank into the cleaning tank, and the microplate was immersed in the disinfecting cleaning solution for 30 minutes to carry out disinfecting cleaning. Discharge and rinse with tap water for 15 minutes in the same manner as above to wash off the washing liquid, and then finish rinsing with demineralized water (flow rate about 20 liters / minute) for 2 minutes, and finally blow with air for 20 minutes to dry and wash. The process ends.

[Washability ... Washing state] In the sterilization washing by the above-mentioned fully automatic microplate washing machine, the state of the washing solution and the state of bubbles adhering to the wells of the microplate are observed, and the following four stages A to D are used. It was evaluated by. A ... Low foaming, good permeability to the surface of the microplate, no air bubbles remained inside the well, and good washability. B: Low foaming, but insufficient cleaning due to slight air bubbles remaining inside the well. C ... A little foaming occurred and the permeability to the surface of the microplate was poor, and some air bubbles remained in the well, resulting in insufficient washing. D: Highly foamed, and considerable air bubbles remained inside the well, resulting in poor cleaning.

[Washability ... Washing Result] The Ponso 3R dyeing solution is applied to the microplate that has been washed by the above-mentioned fully automatic microplate washing machine, left for about 3 minutes, and then rinsed with tap water to remove the Ponso 3R. The presence or absence of red color (coloring of protein stains) was examined, and the results were evaluated according to the following three grades. Good: No residual stain was observed. Slightly defective ... Slight residual stain was observed. Defective: Stain remained remarkably.

[Storage stability] The sterilizing detergent was stored at room temperature (25
It was allowed to stand for 5 months at ~ 30 ° C) and the residual rate of available chlorine was examined.

[Corrosion to Metals] The disinfecting detergent was diluted 30 times with ion-softening water, and the diluted solution was added at 25 ° C. to the SUS304 stainless steel plate (50 × 20).
(× 1 mm) was soaked for 7 days, and then the state of the plate surface was observed.

[0041]

[Table 2]

From the results shown in Table 2 above, N containing an appropriate ratio of the a-component alkyl diphenyl ether disulfonate and the b-component alkyl phenyl ether sulfate ester salt.
o. 3 and No. 3 The disinfectant cleaning agent of No. 4 exhibits excellent disinfectant cleaning properties, can reliably prevent cleaning failure due to air bubbles remaining in the wells, has good storage stability, and maintains high disinfectant cleaning power for a long period of time. It can be seen that it has an ideal quality of being capable of corroding metal and not damaging metal parts such as a cleaning tank and piping. On the other hand, the one containing only the above-mentioned component a (No. 1) is slightly inferior in cleanability,
In the case where only the b component is mixed (No. 7), the foaming property is extremely high, which causes poor cleaning, and in the case where the b component is mixed in a large proportion (Nos. 2, 5, 6), cleaning is caused by residual air bubbles. It is clear that the deterioration of the sex is likely to occur. On the other hand, in the case of containing no polyacrylate (No. 8), the hardness component in water becomes an insoluble substance at the time of washing, the insoluble salt is deposited on the well surface, and white spots appear on the surface of the microplate after drying. In addition, the one containing no silicate (No. 9) has a problem that corrosion of the metal surface occurs.

[0043]

According to the first aspect of the present invention, as a microplate disinfecting detergent for medical tests, it has a strong disinfecting and detergency, and has a stain protein or fat attached to the microplate after the inspection. Can easily decompose and remove the bodily fluid components of B, and can surely kill bacteria and viruses. Moreover, the wettability to the plate surface and the penetrating power to dirt are high, and the air bubbles in the well easily separate, It is possible to provide a cleaning product which is less likely to cause cleaning failure due to residue, has excellent applicability to cleaning by a fully automatic microplate cleaning machine, and has good storage stability.

According to the second aspect of the present invention, as the above-mentioned disinfecting detergent, one having more excellent detergency is provided.

According to the invention of claim 3, as the above-mentioned disinfecting detergent, especially the foaming property is appropriately adjusted, and it is possible to surely prevent the bubbles from remaining in the wells, so that the cleaning failure due to the bubbles can be completely eliminated. What can be prevented is provided.

According to the invention of claim 4, as the above-mentioned disinfecting detergent, there is provided an agent having a good ability to dissolve and remove a metal component in a body fluid adhering to a well of a microplate and to have an excellent washability.

According to the fifth aspect of the present invention, there is provided the above-mentioned disinfecting detergent, which is particularly excellent in storage stability and can maintain a high disinfecting and detergency over a long period of time.

According to the inventions of claims 6 and 7,
As the above-mentioned disinfecting detergent, one having particularly excellent detergency is provided.

According to the eighth aspect of the present invention, there is provided, as the above-mentioned disinfecting detergent, one having a low level of effective chlorine decomposition particularly during storage and having good detergency against dirt of fat and lipid.

[Brief description of drawings]

FIG. 1 is a perspective view of a microplate for medical examination.

FIG. 2 is a perspective view of a fully automatic microplate washing machine.

FIG. 3 is a cross-sectional view showing a disinfecting and washing state by the washing machine.

FIG. 4 is a sectional view of a main part showing a conventional disinfecting and washing state.

[Explanation of symbols]

 1 microplate 2 well 3 washing solution 4 air bubble 10 fully automatic microplate washing machine 11 washing tank

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code Agency reference number FI Technical display location C11D 3/48 C11D 3/48

Claims (8)

[Claims] 1. As essential components, a) alkyl diphenyl ether disulfonate, b) alkyl phenyl ether sulfate, c) hypochlorite, d) polyacrylate, e) silicate, f) hydroxylated. A microplate disinfectant cleaner for medical tests consisting of an aqueous solution containing alkali. 2. The total amount of the alkyl diphenyl ether disulfonate of the component a and the alkyl phenyl ether sulfate ester of the component b in the range of 1 to 20% by weight with respect to the hypochlorite of the component c. The microplate disinfecting detergent for medical examination according to claim 1, which is blended. 3. The medical examination according to claim 1, wherein the compounding ratio of the alkyl diphenyl ether disulfonate as the component a and the alkyl phenyl ether sulfate ester as the component b is 5 to 20 in terms of a / b weight ratio. Microplate disinfectant cleaning agent. 4. The medical examination according to any one of claims 1 to 3, wherein the component (c) is hypochlorite and the component (d) is a polyacrylate in an amount of 1 to 10% by weight. Microplate disinfectant cleaner. 5. The microplate for medical examination according to any one of claims 1 to 4, wherein the silicate of the component e is mixed in the range of 1 to 20% by weight with respect to the hypochlorite of the component c. Disinfectant cleaner. 6. The alkyl diphenyl ether disulfonate as the component a has the following general formula (I); The microplate disinfecting detergent for medical examination according to any one of claims 1 to 4, which is represented by the following formula. 7. The alkylphenyl ether sulfate ester salt of component b has the following general formula (II); The microplate disinfectant cleaning agent for medical examination according to any one of claims 1 to 5, which is represented by the following formula. 8. The alkali hydroxide of component f) is a cleaning agent P.
H is mixed in the range of 11 to 13.
The microplate disinfecting detergent for medical examination according to any one of 5 above.