JPH06319467A - Amino acid-enriched soybean proteinic composition - Google Patents

Abstract

PURPOSE:To provide a water-soluble amino acid-enriched soybean proteinic composition with suppressed solubility. CONSTITUTION:The water-soluble amino acid-enriched soybean proteinic composition comprises (a) a water-soluble amino acid in an amount of <=50wt.% based on the weight of the whole composition and (b) soybean proteins containing least 60wt.% proteins and is obtained by thermally gelatinizing a kneaded material of the water-soluble amino acid with the soybean proteins in a medium of water under conditions of pH 5.0-6.5 and then drying the resultant gelatinized material. Furthermore, the feed for aquatic animals contain this composition is provided.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a soybean protein composition fortified with a water-soluble amino acid. Specifically, such a composition can be used as a feed for aquatic animals.

[0002]

2. Description of the Related Art As a composition in which a water-soluble amino acid is gradually released, a composition in which the water-soluble amino acid is coated with a water-insoluble polymer material such as zein or a cellulose membrane or fats and oils is known. For example, Japanese Patent Application Laid-Open No. 4-173060 discloses a composition for aquatic feed in which a water-soluble amino acid is coated with an oil and fat, so that the content components do not elute, and sustained release and feed efficiency are improved. .

Other compositions for feed for aquatic animals include those containing complex amino acid iron as a main component (see JP-A-59-175851) and liquid egg of fish as an active ingredient. It is known that a protein raw material, vitamins, amino acids, soybean phospholipids, water, etc. are mixed and homogenized, and then dried and powdered (see Japanese Patent Application Laid-Open No. 1-304852). The latter is particularly for shrimp aquaculture. Intended for initial formula feed.

However, although water-insoluble polymers such as cellulose membranes and extremely hardened oils used as coating materials have an excellent effect of sustained release, their digestibility is not always clear. In some cases, the active ingredient may pass through the body while being coated and may not be fully utilized. On the other hand, soybean protein itself has a high nutritional value and can be used as a protein source by itself, so that it can be expected to provide an effective composition.

[0005]

Therefore, an object of the present invention is to carry a water-soluble amino acid, which is particularly effective for aquaculture of aquatic animals, in a state in which it is difficult to easily elute in water (giving sustained release property). An object is to provide an amino acid-enriched composition.

[0006]

[Means for Solving the Problems] The inventors of the present invention have conducted extensive research to impart sustained release properties to water-soluble amino acids,
A sustained-release amino acid-enriched composition can be obtained by treating a soy protein having a specific quality, which has been conventionally used as a binder for various feed compositions, with a certain amount of water-soluble amino acid under certain conditions. They have found the present invention.

Therefore, according to the present invention, there is provided an amino acid-enriched composition in which the solubility of a water-soluble amino acid is suppressed,
(A) 50% by weight or less of the water-soluble amino acid based on the total composition weight, (b) the soybean protein contains at least 60% by weight of protein, and (c) the water-soluble amino acid and the soybean protein. The kneaded product in the water medium of
There is provided a composition characterized by being heat-gelled and dried under a pH condition of 6.5.

Such a composition is advantageously used in the application of the feed for aquatic animals provided as another aspect of the present invention, but the use in other feeds and processed foods is also considered.

[0009]

Specific Embodiments The water-soluble amino acids used in the present invention are those which, when included in a certain composition, are easily eluted into water and include, but are not limited to, arginine, lysine and methionine. Etc. are mentioned as a typical thing. Of these, arginine is particularly preferable when used as a feed for aquatic animals, especially shrimp.

As the soybean protein in which these water-soluble amino acids are kneaded in an aqueous medium, it is necessary to use a soybean protein containing at least 60% by weight of protein. Such soybean protein is not limited depending on its production method, but for example, protein contained in the soybean protein highly enriched in protein content of 60% or more and undenatured defatted soybean powder as raw materials Examples include so-called isolated soybean protein prepared by collecting only soybean protein.

The water-soluble amino acid and the soybean protein are first kneaded in an aqueous medium. The amount of water used is not particularly limited as long as both components are sufficiently homogeneously kneaded. The mixing ratio of these components is 50% by weight of water-soluble amino acid based on the total amount of water-soluble amino acid and soybean protein.
It must be: This is because if it exceeds 50% by weight, the sustained release of the water-soluble amino acid cannot be expected so much.

Next, the kneaded product is heated to gel after being adjusted to a pH of 5.0 to 6.5. This pH range is important because the sustained-release effect of water-soluble amino acids is largely due to the gelation of soybean protein. The heating is not particularly limited as long as the soybean protein is not significantly denatured, but generally 70 ° C to 120 ° C, preferably 85 ° C.
It is preferable to carry out at 100 to 100 ° C.

The gelled composition is then dried. Further, it may be pulverized to prepare a powder having a certain particle size depending on the end use. The degree of drying may be such that it is dried to a water content convenient for grinding, and is usually around 10%. In the case of feed for aquatic animals, the powder particle size is preferably in the range of 50 to 1,000 microns. The aquatic animals referred to in the present invention include, but are not limited to, shrimp, black tiger, yellowtail, eel and the like. The composition of the present invention thus obtained can be used as such as various feeds, but may be mixed with other components if necessary.

[0014]

EXAMPLES The present invention will be described in more detail below with reference to examples. Examples 1 to 7 isolated soybean protein (Adipron-SU, protein content 90%)
80 g, 20 g of arginine hydrochloride and 280 g of water were mixed, hydrochloric acid was added to adjust the pH as shown in Table 1 below, and the mixture was kneaded for 10 minutes, packed in a casing, and heated at 90 ° C. for 30 minutes. 100-150 ° C after shredded heating gel into thin chips
Each composition was obtained by drying to 10% or less of water content in a dry air stream. 1 g of each dry composition was pulverized and classified to have an average particle size of 200 microns and 500 microns.
100 mL of water was placed in a 300 mL Erlenmeyer flask and shaken, and after 1 hour, the concentration of arginine in the supernatant was measured by a colorimetric method (8-oxyquinoline bromination method). This result also shows that the product of the present invention has a lower solubility of water-soluble amino acids than the comparative product.

The dissolution rate was obtained by the following equation, and the results are summarized in Table 1 below. Dissolution rate = Arginine content of supernatant / Arginine content added x 100

[0016]

[Table 1]

Example 8 To 30 g of lysine and 70 g of concentrated soybean protein (adipron S1: protein content 65%), 180 g of water was added, and the pH was adjusted to 5 with sulfuric acid.
After adjusting to 8, a mixed paste was prepared. It was packed in a casing tube, heated at 110 ° C. for 10 minutes, dried in the same manner as in the above example, and pulverized to have an average particle size of 300 μm.
The elution rate of this product into the aqueous solution after 1 hour was 72% of that of untreated lysine. An amino acid analyzer was used to quantify lysine. Arginine strengthening soy protein (pH5.6 the prototype in Application Example Example 3,
Arginine content of 0.5% with an average particle size of 300 microns
A black tiger breeding test was conducted using a feed added so as to increase the weight, and changes in weight gain were investigated. For comparison, the case where crystalline arginine was added as it was and the case where arginine coated with cellulose acetate phthalate (CAP) was evaluated at the same time. The results are shown in Table 2 below. The composition of the control bait is as follows. Composition of feed (control) Fish meal 530 Squid meal 80 Shrimp meal 100 Soybean meal 20 Wheat flour 220 Fish oil 10 Minerals 35 Premix 5 Process Water To do.

[0018]

[Table 2]

The samples in each test section contain arginine in the following amounts. Test Group 1: Control contained 2.0% by weight of arginine as arginine in the protein composition. Test section 2: Control + crystalline arginine (0.5% by weight arginine equivalent) was added. Test Group 3: Control + sample of Example 3 (arginine equivalent to 0.5% by weight) added. Test Group 4: Control + CAP-coated arginine (0.
Addition of arginine equivalent to 5% by weight.

[0020]

INDUSTRIAL APPLICABILITY According to the present invention, the solubility of water-soluble amino acids is suppressed (50 to 70 per hour as compared with the case of no treatment).
%) Amino acid-enriched soy protein composition is provided. Such compositions are particularly useful as feed ingredients for aquatic animals for use in water.

Claims (2)

[Claims] 1. An amino acid-enriched soybean protein composition in which the solubility of a water-soluble amino acid is suppressed, wherein (a) the water-soluble amino acid is 50% by weight or less based on the total weight of the composition, and (b).
The soy protein contains at least 60% by weight of protein, and (c) a kneaded product of the water-soluble amino acid and the soy protein in an aqueous medium is heated under a pH condition of 5.0 to 6.5. A composition characterized by being dried after gelation. 2. An aquatic animal feed comprising the composition according to claim 1.