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JPH06133757A - Device for photosynthetic reaction of fine alga - Google Patents

Device for photosynthetic reaction of fine alga

Info

Publication number
JPH06133757A
JPH06133757A JP28750092A JP28750092A JPH06133757A JP H06133757 A JPH06133757 A JP H06133757A JP 28750092 A JP28750092 A JP 28750092A JP 28750092 A JP28750092 A JP 28750092A JP H06133757 A JPH06133757 A JP H06133757A
Authority
JP
Japan
Prior art keywords
reaction tank
culture solution
photosynthetic reaction
microalgae
photosynthetic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP28750092A
Other languages
Japanese (ja)
Inventor
Masaaki Negoro
正明 根来
Tadayoshi Endo
忠良 遠藤
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Heavy Industries Ltd
Original Assignee
Mitsubishi Heavy Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Heavy Industries Ltd filed Critical Mitsubishi Heavy Industries Ltd
Priority to JP28750092A priority Critical patent/JPH06133757A/en
Publication of JPH06133757A publication Critical patent/JPH06133757A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18Flow directing inserts
    • C12M27/20Baffles; Ribs; Ribbons; Auger vanes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To reduce a site area and convert a photosynthetic reaction tank into a small size by forcibly circulating a nutrient solution in the photosynthetic reaction tank tall in the vertical direction at a high speed and blowing CO2 gas into the reaction tank from its bottom. CONSTITUTION:A nutrient solution is forcibly circulated in a photosynthetic reaction tank 1 tall in the vertical direction at a high speed, and light is uniformly irradiated on algae. A nutrient solution is well supplied to the algae to improve the rate of the photosynthetic reaction. A separation plate 2 is disposed to facilitate the circulation of the nutrient solution and a CO2-containing gas is blown into the culture solution from the lower part of the reaction tank to facilitate the dissolution of CO2 gas.

Description

【発明の詳細な説明】Detailed Description of the Invention

【0001】[0001]

【産業上の利用分野】本発明は微細藻類の光合成反応装
置に関する。
TECHNICAL FIELD The present invention relates to a photosynthetic reaction device for microalgae.

【0002】[0002]

【従来の技術】従来技術による微細藻の生産方法及び生
産装置を図4によって説明する。従来は光合成反応槽1
の中に供給液ポンプ14で培養液3と種となる少量の微
細藻4を導入し、光合成反応槽1の表面から太陽光を受
けて光合成反応を進行させて微細藻4を増殖させてい
る。光合成に必要な炭酸ガス(CO2 )はブロワ18か
ら散気装置19を介して反応槽1の中の培養液3に溶解
させ、培養液の混合攪拌は攪拌機13で行なわれ、通常
は羽根付きの水車(パドルフィール)が使用されてい
る。微細藻4が増殖すれば一定期間ごとに回収ポンプ1
6で生産藻体を回収する。生産藻体は培養液と共に回収
するのでその量分だけ供給液ポンプ14で培養液3のみ
を補充するいわゆる半連続運転方法か、あるいは連続的
な供給と抜出しを行う連続運転方法が採用されている。
なお、従来技術の光合成反応槽1は図4に示すように水
深が浅く(通常20〜50cm)表面積を広くとる構造
になっており、培養液3の攪拌混合方式は上記のパドル
フィールによって行われるが、その攪拌速度は遅く、毎
秒10〜20cm程度である。なお、図4中、21は光
合成反応部を示す。
2. Description of the Related Art A conventional method and apparatus for producing microalgae will be described with reference to FIG. Conventionally photosynthetic reaction tank 1
The culture solution 3 and a small amount of seed microalgae 4 are introduced by a supply liquid pump 14 into the inside, and the photosynthetic reaction is progressed by receiving sunlight from the surface of the photosynthesis reaction tank 1 to grow the microalgae 4. . Carbon dioxide (CO 2 ) required for photosynthesis is dissolved in the culture solution 3 in the reaction tank 1 from the blower 18 through the air diffuser 19, and the mixing and stirring of the culture solution is performed by the stirrer 13, usually with a blade. The water wheel (paddle feel) of is used. If the microalgae 4 grows, the collection pump 1
In step 6, the produced algal cells are collected. Since the produced algal cells are collected together with the culture solution, a so-called semi-continuous operation method in which only the culture solution 3 is replenished by the supply solution pump 14 in the amount, or a continuous operation method in which continuous supply and extraction are performed is adopted. .
As shown in FIG. 4, the prior art photosynthetic reaction tank 1 has a structure with a shallow water depth (usually 20 to 50 cm) and a large surface area, and the stirring and mixing method of the culture solution 3 is performed by the above paddle feel. However, the stirring speed is slow and is about 10 to 20 cm per second. In addition, in FIG. 4, 21 shows a photosynthesis reaction part.

【0003】[0003]

【発明が解決しようとする課題】従来技術は前述のとお
り水深が浅く光が当たる表面積が広い光合成反応槽であ
る。微細藻の増殖(生産)は次の反応式で示すように、
その基本反応は炭酸ガス(CO2 )と水(H2 O)から
太陽エネルギ(波長:400〜700nmの可視光)を
得て糖(C6 126 )を合成しこれから藻体が生合成
されるものである。 6CO2 +6H2 O → C6 126 +6O2
(光の存在下)
As described above, the prior art is a photosynthesis reaction tank having a shallow water depth and a large surface area exposed to light. The growth (production) of microalgae is as shown in the following reaction formula:
The basic reaction is to obtain solar energy (visible light with a wavelength of 400 to 700 nm) from carbon dioxide (CO 2 ) and water (H 2 O) to synthesize sugar (C 6 H 12 O 6 ), from which alga is produced. It is synthesized. 6CO 2 + 6H 2 O → C 6 H 12 O 6 + 6O 2
(In the presence of light)

【0004】藻体が分裂して増殖すると、これが光の水
中への透過を妨げるので光合成反応が進まなくなる。し
たがって光合成反応槽は水深を深くすることができず、
光が透過できるような水深の浅いものにあらざるを得な
くなり、微細藻を大量に生産する場合には表面積の広い
光合成反応槽が必要であり膨大な立地面積が要求され
る。
When the alga body divides and proliferates, it impedes the transmission of light into water and the photosynthetic reaction does not proceed. Therefore, the photosynthetic reaction tank can not deepen the water depth,
There is no choice but to use a shallow water depth that allows light to pass through, and a large-area photosynthetic reaction tank is required when producing a large amount of microalgae, which requires a huge site area.

【0005】本発明は上記技術水準に鑑み、水深の大な
る構造にして、その立地面積を低減することができる微
細藻類の光合成反応槽を提供しようとするものである。
In view of the above-mentioned state of the art, the present invention aims to provide a photosynthetic reaction tank for microalgae which has a structure with a large depth of water and whose site area can be reduced.

【0006】[0006]

【課題を解決するための手段】本発明は (1)培養液及び種となる微細藻類を供給する手段と増
殖した微細藻類を回収する手段を有する垂直方向に背の
高い光合成反応槽、該反応槽を上部と下部に一端を開け
て分割する静置あるいは可動式の隔離板、下部の培養液
を前記隔離板の上部に直接または堰を介して移送する手
段及び下部の培養液中にCO2 含有ガスを供給する手段
を具備してなることを特徴とする微細藻類の光合成反応
装置。 (2)隔離板が角度を任意に変えうる傾斜型隔離板であ
ることを特徴とする上記(1)記載の微細藻類の光合成
反応装置。 である。
The present invention provides (1) a vertically tall photosynthetic reaction tank having means for supplying a culture solution and seed microalgae and means for recovering grown microalgae, and the reaction. A stationary or movable separator that divides the tank into upper and lower parts with one end opened, means for transferring the lower culture solution directly or through a weir, and CO 2 in the lower culture solution A photosynthetic reaction device for microalgae, comprising a means for supplying a contained gas. (2) The photosynthetic reaction device for microalgae according to (1) above, wherein the separator is an inclined separator whose angle can be changed arbitrarily. Is.

【0007】本発明装置は光の当る表面積は一定にして
光合成を行わせる反応部は従来と同様かあるいはそれよ
り浅い水深とするが、反応槽全体は光の当る垂直方向に
背の高い形状とし、この反応槽の表面部分で光合成反応
を行わせるが下槽部にある培養液をこの表面部分に送り
上層部と下層部の液を強制的に循環させるようにしたも
のである。この培養液循環を容易に行わせるために上部
には光合成反応部の底にもなる板を配置する。これによ
って光合成反応部を流れる培養液の流速は速くなり、下
層部にある培養液との入れ替えが頻繁に行われるように
するものである。この循環流速は従来技術の場合よりも
速く急速攪拌とし、例えば1m/sec以上の速さにす
るのが好ましい。さらに、また上部の底板には突起部分
を設けるなどしてできる限り流れを攪乱させる構造物と
するのが好ましい。
In the apparatus of the present invention, the surface area exposed to light is constant, and the reaction section for performing photosynthesis is the same as or has a shallower water depth than the conventional one, but the entire reaction vessel has a tall shape in the vertical direction exposed to light. The photosynthetic reaction is carried out on the surface portion of this reaction tank, but the culture solution in the lower tank portion is sent to this surface portion to forcibly circulate the liquid in the upper layer portion and the lower layer portion. In order to facilitate the circulation of the culture solution, a plate which also serves as the bottom of the photosynthetic reaction section is arranged at the upper part. As a result, the flow rate of the culture solution flowing through the photosynthesis reaction section is increased, and the culture solution in the lower layer is frequently replaced. The circulation flow velocity is faster than that in the case of the conventional technique and rapid stirring is performed, and for example, it is preferable to set the speed to 1 m / sec or more. Furthermore, it is preferable that the upper bottom plate is provided with a protrusion so that the structure disturbs the flow as much as possible.

【0008】[0008]

【作用】光合成反応槽の表面積を一定にすると、ここに
照射される太陽エネルギの量は一定であるが培養液を表
層部に持上げて強制循環させることにより新しい藻が光
の当る部分に順次継続的に供給され、光が無駄なく有効
に利用される。
[Function] When the surface area of the photosynthetic reaction tank is constant, the amount of solar energy applied to the photosynthetic reaction tank is constant. Light is efficiently supplied without waste.

【0009】また深い光合成反応槽の底部からCO2
吹込むことにするため、吹込まれたCO2 ガス気泡の上
昇距離が長く(上昇時間も長く)なるので、CO2 ガス
の溶解効率が高くなりCO2 の利用効率が向上する。ま
た、上層部の光合成反応に関与する培養液を高流速で攪
乱させることにより微細藻と培養液の接触が高効率に行
われる。
Further, since CO 2 is blown from the bottom of the deep photosynthesis reaction tank, the rising distance of the blown CO 2 gas bubbles is long (the rising time is also long), so that the dissolution efficiency of CO 2 gas is high. The utilization efficiency of CO 2 is improved. In addition, by disturbing the culture solution involved in the photosynthetic reaction of the upper layer at a high flow rate, the contact between the microalgae and the culture solution is performed with high efficiency.

【0010】[0010]

【実施例】【Example】

(実施例1)本発明の一実施例を図1によって説明す
る。光合成反応槽1に培養液3が満たされ、微細藻4が
存在する。隔離板2で光合成反応槽1の上部と下部を区
切ることとし、モータ11駆動による水中ポンプ10で
培養液3を循環させる。CO2はブロワ18により散気
装置19を介して光合成反応槽1へ吹込ませる。光合成
反応部21は水深が浅く、またこの部分の液流速を高速
度にする。駆動装置30は隔離板2の位置を上下に稼動
させるためのものである。
(Embodiment 1) An embodiment of the present invention will be described with reference to FIG. The photosynthetic reaction tank 1 is filled with the culture solution 3 and the microalga 4 is present. The upper and lower parts of the photosynthesis reaction tank 1 are separated by the separator plate 2, and the culture solution 3 is circulated by the submersible pump 10 driven by the motor 11. CO 2 is blown into the photosynthesis reaction tank 1 by the blower 18 through the air diffuser 19. The photosynthesis reaction section 21 has a shallow water depth, and the liquid flow velocity in this section is high. The drive device 30 is for moving the position of the separator 2 up and down.

【0011】(実施例2)本発明の他の実施例を図2に
よって説明する。隔離板2とともに堰25を設置し、ポ
ンプ12で培養液3を堰25に送りオーバーフローする
培養液3は光合成反応部21を流れて再び光合成反応槽
1に戻るようにする。隔離板2は水平あるいは少し傾斜
させて培養液が流れやすいように設置するのが好まし
い。培養液供給ライン15は生産藻体を回収ポンプ16
で引抜いた時に同量分を供給するためのものである。
(Embodiment 2) Another embodiment of the present invention will be described with reference to FIG. The weir 25 is installed together with the separating plate 2, and the culture solution 3 is sent to the weir 25 by the pump 12 so that the overflowing culture solution 3 flows through the photosynthesis reaction section 21 and returns to the photosynthesis reaction tank 1 again. The separator 2 is preferably installed horizontally or slightly inclined so that the culture solution can easily flow. The culture solution supply line 15 has a pump 16 for collecting the produced algal cells.
It is for supplying the same amount when pulled out.

【0012】(実施例3)本発明のさらに異なる実施例
を図3よって説明する。図3は実施例2の装置(図2)
の変形装置であり、図2と同一部分には同一符号を付し
てある。この実施例装置は隔離板2を大きく傾斜させポ
ンプ12によって循環する培養液3を堰25を介して急
速に落下させるようにしたものであり、この隔離板2は
図示した固定壁55に沿って壁面昇降装置51によって
可動壁56を昇降自在になるようにされるほか、角度調
整装置50によって隔離板2を変えるようになってい
る。このように構成することにより、光合成反応部21
の液は大きく乱れを起こし、また培養液の厚みを小さく
することができるばかりでなく、太陽光の位置により光
合成反応部の光照射面積(反応槽1の据付面積当りの)
を大きくすることができる。なお、図3中、17は排出
ラインで、こゝから増殖した微細藻と培養液が回収され
る。
(Embodiment 3) Still another embodiment of the present invention will be described with reference to FIG. FIG. 3 shows an apparatus of Example 2 (FIG. 2)
2 and the same parts as those in FIG. 2 are denoted by the same reference numerals. In the apparatus of this embodiment, the separator 2 is largely tilted so that the culture solution 3 circulated by the pump 12 is rapidly dropped through the weir 25. The separator 2 extends along the illustrated fixed wall 55. The wall elevating device 51 allows the movable wall 56 to move up and down, and the angle adjusting device 50 changes the separator 2. With this configuration, the photosynthesis reaction section 21
Not only can the liquid of the culture be greatly disturbed and the thickness of the culture liquid can be reduced, but also the light irradiation area of the photosynthesis reaction section (per installation area of the reaction tank 1) depending on the position of sunlight.
Can be increased. In addition, in FIG. 3, 17 is a discharge line, from which microalgae and culture liquid that have grown are collected.

【0013】[0013]

【発明の効果】本発明装置によって下記の効果が奏され
る。 (1)培養液の強制的高流速循環により、光合成反応速
度が大になり、結果的に光合成反応槽の小型化が計れ
る。 (2)深層型反応槽であるから据付面積が小さくなり、
また、水深の大な培養液底部からCO2 を吹込むのでC
2 溶解効率が高い。
The device of the present invention has the following effects. (1) The photosynthetic reaction rate is increased by the forced circulation of the culture solution at a high flow rate, and as a result, the photosynthetic reaction tank can be downsized. (2) Since it is a deep reactor, the installation area is small,
Also, since CO 2 is blown from the bottom of the culture solution at a deep water depth, C
O 2 dissolution efficiency is high.

【図面の簡単な説明】[Brief description of drawings]

【図1】本発明の微細藻の光合成反応槽の一実施例の説
明図。
FIG. 1 is an explanatory view of an embodiment of a photosynthetic reaction tank for microalgae of the present invention.

【図2】本発明の微細藻の光合成反応槽の他の実施例の
説明図。
FIG. 2 is an explanatory view of another embodiment of the photosynthetic reaction tank for microalgae of the present invention.

【図3】本発明の微細藻の光合成反応槽のさらに他の実
施例の説明図。
FIG. 3 is an explanatory view of still another embodiment of the photosynthetic reaction tank for microalgae of the present invention.

【図4】従来の微細藻の光合成反応槽の一態様の説明
図。
FIG. 4 is an explanatory diagram of one mode of a conventional photosynthesis reaction tank for microalgae.

Claims (2)

【特許請求の範囲】[Claims] 【請求項1】 培養液及び種となる微細藻類を供給する
手段と増殖した微細藻類を回収する手段を有する垂直方
向に背の高い光合成反応槽、該反応槽を上部と下部に一
端を開けて分割する静置あるいは可動式の隔離板、下部
の培養液を前記隔離板の上部に直接または堰を介して移
送する手段及び下部の培養液中にCO 2 含有ガスを供給
する手段を具備してなることを特徴とする微細藻類の光
合成反応装置。
1. A culture solution and a seed microalgae are supplied.
Vertical method with means and means for collecting grown microalgae
A photosynthetic reaction tank that is taller than the other
Stationary or movable separator that opens at the end and divides, lower part
Transfer the culture solution to the upper part of the separator directly or through a weir.
CO in the culture solution in the lower part 2Supply containing gas
Light of microalgae characterized by comprising means for
Synthetic reactor.
【請求項2】 隔離板が角度を任意に変えうる傾斜型隔
離板であることを特徴とする請求項1記載の微細藻類の
光合成反応装置。
2. The photosynthetic reaction device for microalgae according to claim 1, wherein the separator is an inclined separator whose angle can be arbitrarily changed.
JP28750092A 1992-10-26 1992-10-26 Device for photosynthetic reaction of fine alga Withdrawn JPH06133757A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP28750092A JPH06133757A (en) 1992-10-26 1992-10-26 Device for photosynthetic reaction of fine alga

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP28750092A JPH06133757A (en) 1992-10-26 1992-10-26 Device for photosynthetic reaction of fine alga

Publications (1)

Publication Number Publication Date
JPH06133757A true JPH06133757A (en) 1994-05-17

Family

ID=17718152

Family Applications (1)

Application Number Title Priority Date Filing Date
JP28750092A Withdrawn JPH06133757A (en) 1992-10-26 1992-10-26 Device for photosynthetic reaction of fine alga

Country Status (1)

Country Link
JP (1) JPH06133757A (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003006629A1 (en) * 2001-07-11 2003-01-23 Toshirou Sekine Culturing method and device for photosynthetic microbes
WO2009134114A1 (en) * 2008-04-30 2009-11-05 Tet Shin Ho An apparatus for mass cultivation of micro algae and a method for cultivating the same
US20110078949A1 (en) * 2008-06-05 2011-04-07 Alge Oil Gmbh & Co. Kg Breeding and reproduction system for light-intensive microorganisms (such as algae)
US8062880B2 (en) 2007-04-13 2011-11-22 Freeman Energy Corporation Biomass cultivation system and corresponding method of operation
WO2012050221A2 (en) * 2010-10-13 2012-04-19 Sekine Toshirou Culturing method and device for photosynthetic microorganism
CN104787892A (en) * 2015-03-27 2015-07-22 沈阳航空航天大学 Closed ecological treatment system for microalgae wastewater in nano biological membrane
JP2016096769A (en) * 2014-11-20 2016-05-30 花王株式会社 Method of culturing fine algae
JP2020022386A (en) * 2018-08-07 2020-02-13 浜松ホトニクス株式会社 Culture apparatus

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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