JPH05256853A - Automatic analyzer - Google Patents

Abstract

PURPOSE:To improve operability and miniaturize a device by diluting a standard sample with a known concentration to an arbitrary dilution magnification and using it as a standard sample and then reducing the type of standard sample to be prepared. CONSTITUTION:A specified amount of standard sample (STD) is dispensed into a reaction container 2 by a pipetting mechanism 7. Then, a reaction disk 1 is rotated and then the container 2 is carried to a reagent pipetting device. Then, a specified amount of dilution liquid of a dilution cup on a reagent disk 9 is sucked by a pipetting mechanism 10B and is discharged to the container 2. The STD and the dilution liquid are stirred and blended by a stirring mechanism 11, thus obtaining a diluted STD. Then, the disk 1 is rotated and the container 2 is transported. Reagents which are needed for analysis are added to the container 2 with the STD by using reagent pipetting mechanism 10A and 10B for reaction, thus measuring absorbance by using a multiple wavelength photometer 12. A calibration curve is created by the absorbance which is taken in.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic analyzer for preparing a multipoint calibration curve using a standard sample solution.

[0002]

2. Description of the Related Art A conventional automatic analyzer is required to analyze a standard sample having a known concentration for each analysis item to prepare a multipoint calibration curve. The types of standard sample liquids required when creating a calibration curve vary from one type to several types for one analysis item. These standard sample liquids must be prepared by diluting the standard sample liquids by an operator and installed in the apparatus, which requires a large amount of labor and is likely to cause an operational error. Further, a space for accommodating many kinds of standard sample liquids is required, which causes a problem that the device becomes large.

Regarding automatic preparation of standard sample solution,
As described in JP-A-64-43759, a standard sample solution having a plurality of concentrations is dispensed by dispensing different amounts of the standard sample solution and a fixed amount of the analytical reagent, and mixing the standard sample solution with the analytical reagent. There is a way to get the reaction.

[0004]

In the above-mentioned prior art, a standard sample solution is usually used in a high concentration. Therefore, in preparing a multi-point calibration curve, it is necessary to use a wide range of concentrations. In order to obtain a low-concentration standard sample solution, the dispensed amount is very small. For this reason, there is a problem that a highly precise microdispensing is required, and an accurate dispensing accuracy cannot be obtained. Furthermore, since the amount of the reaction liquid changes depending on the dispensed amount of the standard sample liquid, a photometric system mechanism that can perform highly accurate measurement even if the amount of the reaction liquid changes greatly is required.

An object of the present invention is to obtain a standard sample solution having a wider range of concentration than one standard sample solution and prepare a multipoint calibration curve in accordance with the performance of the dispensing mechanism and the photometric system mechanism of the apparatus. It is to provide an automatic analyzer.

[0006]

In order to achieve the above object, an arbitrary amount of standard sample solution is diluted with an arbitrary amount of diluent to prepare a standard sample solution of desired concentration, and after this dilution This is a method for preparing a multi-point calibration curve using the standard sample solution of.

Further, the input position of the standard sample solution in the device for obtaining an arbitrary concentration for each analysis item by the input / output device,
A screen for inputting the dispensing amount of the standard sample solution and the dispensing amount of the diluting solution is provided, and the standard sample solution is diluted according to the information input from the screen in the calibration curve creation mode.

[0008]

In the automatic analyzer according to the present invention, in order to prepare the standard sample solution having the target concentration by the ratio of the dispensed amount of the standard sample solution and the diluted solution amount, the dispensing accuracy of the dispenser mechanism of the device is adjusted. , You can set the dispensing amount. Further, by re-dispensing the diluted standard sample solution as the standard sample solution, the amount of the reaction solution can be arbitrarily set according to the performance of the photometric system mechanism. This makes it possible to create a calibration curve with a small number of standard sample solutions.

[0009]

Embodiments of the present invention will be described below with reference to the drawings.

FIG. 1 is a block diagram of the principle of an automatic analyzer. In FIG. 1, 1 is a reaction disk, and a reaction container 2 is provided on the outer periphery of the reaction disk 1. The whole reaction disk 1 is kept at a predetermined temperature by a heat retaining tank 3.

Reference numeral 5 denotes a sample sample disk mechanism, and a large number of sample cups 6 are installed in this mechanism 5. The sample in the sample cup 6 is appropriately extracted by the nozzle 8 of the sample pipetting mechanism 7 and injected into the reaction container at the sample dispensing position 4. Reference numeral 9 is a reagent disk mechanism in which a reagent cup is installed, and each reagent disk mechanism 9 is equipped with a reagent pipetting mechanism 10A and 10B. A stirring mechanism 11 is arranged close to the reagent disc mechanism 9. Reference numeral 12 is a multi-wavelength photometer, 13 is a light source, and the reaction container 2 for accommodating the object of photometry is arranged between the multi-wavelength photometer 12 and the light source 13. 14 is a cleaning mechanism. Regarding the control system and the signal processing system, 15 is a microcomputer, 16 is an interface, and 17 is Log (logarithm).
Converter, 18 is an A / D converter, 19 is a reagent dispensing mechanism, 2
Reference numeral 0 is a washing pump, and 21 is a reagent dispensing mechanism. Again 22
Is a printer, 23 is a CRT, 24 is a floppy disk as a storage device, and 25 is an operation panel.

The sample placed in the sample cup 6 in FIG.
Microcomputer 15 input from the operation panel
A predetermined amount is dispensed into the reaction container 2 using the nozzle 8 of the sample pipetting mechanism 7 according to the analysis parameter stored in the internal memory.

Next, the reaction container 2 in which the sample has been dispensed is transferred to the reagent dispensing position by rotating the reaction disk 1. After that, according to the analysis parameter stored in the microcomputer 15 which is input from the operation panel, the reagent is supplied to the reaction container 2 into which the sample is dispensed using the nozzles of the reagent pipetting mechanisms 10A and 10B. Dispense. Then, the stirring mechanism 11 stirs and mixes the sample and the reagent. When the reaction container 2 crosses the photometric position, the absorbance is measured by the multi-wavelength photometer 12.
The measured absorbance is the multiplexer, Log conversion 17,
Via A / D converter 18 and interface 16,
It is taken into the microcomputer 15. This absorbance is based on the calibration curve created from the absorbance of the standard sample solution that has been measured by the analytical method specified in advance for each item,
Converted to density data. This component concentration data is output to a printer or screen.

In the above measurement principle, the accuracy of the calibration curve has a great influence on the measurement accuracy of the concentration data.

FIG. 2 shows an example of details of a method for realizing claim 1 of the present invention. In step 1, the standard sample solution (hereinafter STD
From the sample cup 6 in which 1 is omitted), a predetermined amount of STD 1 is dispensed into the reaction container 2 from the nozzle of the sample pipetting mechanism 7. After that, the reaction disk is rotated by a half turn and one reaction container, and the reaction container 2 containing STD1 is transferred to the reagent pipetting position. In step 2, a predetermined amount of the diluting liquid set in the diluting cup on the reagent disk 9 is sucked from the nozzle of the pipetting mechanism 10B and discharged into the reaction container containing STD1. The standard sample solution and the diluting solution are agitated by the agitation mechanism 11 to be mixed, and diluted STD
Get one. Then, the reaction disk is rotated by a half turn and one reaction container, and the reaction container 2 containing the diluted STD1 is transferred from the sample dispensing position 4 to a position two reaction containers ahead. In step 3, a predetermined amount of diluted STD1 is sucked and discharged into the reaction container at the sample dispensing position 4. This diluted ST
A reagent required for analysis is added to the reaction container 2 containing D1 and reacted, and the absorbance is measured by the multi-wavelength photometer 12, and a calibration curve is created by the taken absorbance.

According to the present embodiment, the standard sample solution having an arbitrary concentration can be obtained by diluting the standard sample solution at an arbitrary dilution ratio with the conventional apparatus configuration without adding a special dilution mechanism. You can

3 and 4 show the second embodiment of the present invention, which will be described below.

Dispensing parameters of the standard sample solution necessary for preparing a calibration curve for each analysis item are displayed on the analysis parameter screen as shown in FIG. To enter this screen, first, the analysis item 26 and the analysis method 27 are input. Next, the concentration value 28 and the standard sample dispensing amount 29 to the reaction container are input, and the installation position 30 of the standard sample liquid on the apparatus and the standard sample liquid dispensing amount (standard sample liquid before dilution, which are the features of the present invention, are input. Enter the amount 31 and the diluted liquid dispensed amount 32. The information input on this screen is stored in the storage device.

FIG. 4 is a process flow chart of claim 2 of the present invention. If you want to create a calibration curve, specify the calibration curve creation mode 33 and read the analysis parameters from the storage device 34. Determine whether there is a dilution specification 35 for each standard sample of each analysis item. If the dilution is not designated, the designated standard sample 36 is dispensed 37 into the reaction container.
When the dilution is designated, the designated standard sample 38 is dispensed 41 into the dilution container in the designated amount. Then, the designated amount of the diluting liquid is dispensed 40 into the diluting container. Dispense the diluted standard sample solution into the designated volume 41. Each standard sample dispensed into the reaction vessel is measured 42 and stored 43. These are repeated 44 times a specified number of times of calibration data. After that, read the data stored when the predetermined number of measurement data was obtained,
A calibration curve is created.

From the above, according to the performance of the dispensing mechanism and the photometric system mechanism of the device, it is possible to obtain a standard sample liquid having a wider range of concentration than one standard sample liquid and prepare a multipoint calibration curve. Became.

[0021]

According to the present invention, a standard sample having a known concentration is used as a standard sample while being diluted at an arbitrary dilution ratio, thereby reducing the types of standard samples to be prepared and improving the operability. It is possible to reduce the size of the automatic analyzer.

[Brief description of drawings]

FIG. 1 is a configuration diagram of an automatic analyzer according to an embodiment of the present invention.

FIG. 2 is a diagram showing an example of a method for diluting a standard sample.

FIG. 3 is a diagram showing an analysis parameter setting screen.

FIG. 4 is a processing flowchart.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Reaction disk, 2 ... Reaction container, 5 ... Sample sample disk mechanism, 6 ... Sample cup, 7 ... Sample pipetting mechanism, 8 ... Nozzle, 10B ... Reagent pipetting mechanism,
12 ... Multi-wavelength photometer, 13 ... Light source, 15 ... Microcomputer, 19 ... Reagent dispensing mechanism, 21 ... Sample dispensing mechanism,
23 ... CRT.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁵ Identification number Internal reference number FI Technical indication location G01N 21/27 F 7370-2J (72) Inventor Hiroshi Mimaki 882 Ichige, Ichige Katsuta, Ibaraki Stock Hitachi, Ltd. Measuring Instruments Division

Claims (2)

[Claims] 1. An analysis mechanism having a sample dilution function, a storage device for storing measured data and analysis conditions, an input / output device for inputting / outputting measurement data analysis conditions, the analysis mechanism, storage device, In an automatic analyzer that consists of a central processing unit that controls the input / output device, one standard sample solution is diluted with an arbitrary dilution ratio by changing the amounts of standard sample solution and diluting solution to obtain standard sample solutions of various concentrations. An automatic analyzer characterized in that it obtains a multipoint calibration curve. 2. The automatic analyzer according to claim 1, wherein a screen for inputting an installation position of the standard sample on the apparatus and a dispensed amount of the standard sample solution and a diluent is provided for each measurement item by an input / output device. , The information input on this screen is stored in the storage device, the information is read from the storage device in the calibration curve creation mode, and the standard sample solution is diluted according to the information to obtain standard samples of various concentrations. An automatic analyzer characterized by creating a multi-point calibration curve.