JPH05157747A - Blood-sampling tube - Google Patents

Abstract

PURPOSE:To shorten time for coagulation after blood sampling to shorten time for blood inspection thereby and to enable sure execution of separation of clot from serum by a method wherein a blood coagulation accelerating medium impregnated with an enzymic blood coagulation accelerator is held in the central part inside a tube. CONSTITUTION:In this blood-sampling tybe, a blood coagulation accelerating medium which is a carrier having a higher specific gravity than blood and is impregnated with an enzymic blood coagulation accelerator is held in the central part inside the tube. As for the coagulation accelerator, an enzymic agent such as thermobin or venom enzyme is used therefor. The blood-sampling tube may be made of either glass or synthetic resin. In order to make the blood coagulation accelerating medium be held in the central part inside the tube, a support such as nonwoven fabric, filter paper or cloth is used. The nonwoven fabric or the like is used for holding the blood coagulation accelerating medium between tube walls by utilizing the rigidity or elasticity thereof. The support needs to have no hemolytic property, to produce no adverse effect on biochemical inspection, to have such flexibility as to be movable to a hemocyte layer by a stress at the time of centrifugation, and to have such a size as to allow it to sink into the hemocyte layer.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood collection tube. More specifically, it relates to a blood collection tube capable of coagulating blood.

[0002]

2. Description of the Related Art When serum is centrifuged from collected whole blood, if the blood coagulation is insufficient, a boundary surface having a so-called intermediate specific gravity is formed poorly after centrifugation, and when the blood collection tube is laid down sideways, blood cells become serum. There are problems such as leakage to the layers and generation of fibrin, which limits the amount of serum collected.

Therefore, conventionally, 45 minutes to 1 and a half hours after blood collection is sufficiently coagulated and then centrifuged, but still, due to individual differences, sufficient coagulation cannot be obtained and fibrin is deposited. There is.

Such a long coagulation time is not preferable from the viewpoint of efficiency of serum separation operation and blood test. Therefore, various measures have been conventionally proposed for promoting blood coagulation. For example, a method of coating a container in which glass powder is dispensed with a water-soluble polymer on the inner wall of a blood collection tube, a method of disposing a container in which glass powder is dispensed in a blood collection tube, and the like are known.

However, the former method is complicated in the steps of coating and drying, and is not preferable in the working environment because it uses an organic solvent, and the latter method requires a container for containing glass powder, which is costly. However, there is a problem in that it is difficult to manage for dispensing an appropriate amount of powder.

In order to solve such a problem, a blood collection tube containing a blood coagulation accelerating medium in a tube, the blood coagulation accelerating medium being a carrier having a higher specific gravity than blood and powdered through a binder soluble in serum. A blood collection tube carrying a blood coagulation accelerator is disclosed in Japanese Patent Publication No. 58-27933.

[0007]

DISCLOSURE OF THE INVENTION Problems to be Solved by the Invention
Even with a blood collection tube containing a coagulation promoter disclosed in Japanese Patent No. 933 or a combination of this tube and a separating agent, the coagulation time takes about 15 minutes, and the coagulation agent containing an anticoagulant such as a dialysis patient sample is included. It takes about 40 minutes for each sample,
Inadequate for rapid blood tests.

In this Japanese Patent Publication No. 58-27933, it is considered that the powdery blood coagulation promoter is selected from glass, kaolin, bentonite diatomaceous earth, diatomaceous earth and silica sand, so that the coagulation time is long.

Further, regarding a more rapid blood test, JP-A-56-106157 and JP-A-57-57
Japanese Patent No. 142561 discloses that a blood coagulation promoter for serum separation containing a venom such as Himehab as an active ingredient is preferable.

However, in this case, if there is a serum separating agent in the blood collection tube, this adsorbs or masks the snake venom enzyme,
There has been a problem that the function of the enzyme is diminished, for example, the dispersion of the enzyme is deteriorated.

The present invention provides a blood collection tube that coagulates blood in a shorter time than when a powdery blood coagulation promoter is used and is not affected by the serum separating agent in the blood collection tube.

[0012]

The present invention is a carrier having a specific gravity higher than that of blood, and is characterized in that a blood coagulation promoting medium impregnated with an enzyme-based blood coagulation promoting agent is contained in a central portion of a tube. The main point is blood vessels.

In the present invention, the fact that the blood coagulation promoting medium is contained in the central portion of the tube is to prevent the blood coagulation promoting medium and the serum separating agent at the bottom of the tube from contacting each other in the tube. It is sufficient if the separating agents are in a position where they do not contact each other.

[0014]

In the present invention, at the time of collecting blood, an enzyme-based accelerator that promptly promotes blood coagulation is dissolved in the blood to rapidly coagulate the blood. Also, in the present invention, since the blood coagulation promoter is present in the central portion of the tube separated from the serum separating agent by the support, the blood coagulation function can be sufficiently exerted.

[0015]

EXAMPLES Examples of the present invention will be described below. INDUSTRIAL APPLICABILITY The present invention is suitable as a blood collection tube with a sealant (for example, a rubber stopper) that a serum separating agent has, and thrombin as a coagulation accelerator,
Snake venom enzyme (reptilase, crotarose, ocord)
Enzyme-based drugs such as A well-known thixotropic gel-like substance is often used as the serum separating agent, and the blood collection tube may be made of glass or synthetic resin.

In the present invention, a support such as a non-woven fabric, a filter paper or a cloth is used to contain the blood coagulation promoting medium in the central portion of the tube, and the support is impregnated with the enzyme-based blood coagulation promoting agent. The non-woven fabric or the like is used as the support in order to hold the blood coagulation promoting medium between the tube walls by utilizing its rigidity or elasticity. In addition to having rigidity or elasticity, the support does not have hemolytic properties-It does not adversely affect biochemical tests-It has the flexibility to move to the blood cell layer due to the stress during centrifugation-The blood cell layer It is necessary that the size is such that it can sink inside.

When a blood coagulation promoter for serum separation containing venom of Himehab as an active ingredient is used, it is disclosed in JP-A-56-106.
With reference to Japanese Patent No. 157, it is manufactured as follows.

A snake venom was discharged from a living Himebu on a sheare, and a large amount of this was collected and cooled by a cooling centrifuge.
Centrifugation is performed at about C, and the supernatant is freeze-dried, and the freeze-dried product is used as a blood coagulation promoter.

The lyophilized product obtained here is dissolved in physiological saline in a necessary amount at the time of use, adjusted to a concentration similar to that of the snake venom, and passed through a filter membrane having a pore size of about 0.45 μm to prepare a sterile coagulation enzyme solution. To do.

Stabilizers and dispersants may be added to the sterile coagulation enzyme solution. The enzyme solution is impregnated with a support, and 0.2 NIH unit / ml is used per 1 ml of the blood sample. The NIH unit is an active unit for coagulating 1 ml of 0.1% fibrinogen solution in 15 seconds.

The blood collection tube containing the medium impregnated with the enzyme-based coagulation accelerator of the present invention or the combination of this tube and the separating agent has a coagulation time of 5 minutes or less, and is used for dialysis patient specimens and the like. 15 to 2 for samples containing anticoagulant
A blood test could be performed quickly, requiring only 0 minutes.

[0022]

EFFECTS OF THE INVENTION By using the blood collection tube of the present invention, the coagulation time after blood collection can be shortened to about 5 minutes, and blood containing an anticoagulant can be dealt with. Shortened. Further, in the present invention, the blood coagulation promoting medium is provided in the central portion of the tube, and the blood clot and the serum can be reliably separated.

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Claims (1)

[Claims] 1. A blood collection tube which is a carrier having a specific gravity higher than that of blood and which contains a blood coagulation promoting medium impregnated with an enzyme-based blood coagulation promoting agent in a central portion of a tube.