JP7526572B2 - Sampling device, culture device, and method for producing culture - Google Patents

Description

The present invention relates to a sampling device, a culture device, and a method for producing a culture.

In the manufacture of pharmaceuticals, foods, cosmetics, chemical substances, etc., the operation of placing liquid raw materials, culture medium, etc. in a container and stirring is often used. For example, when culturing a culture of microorganisms, cells, etc., or when obtaining a culture product such as a substance produced by the cultured microorganisms, cells, etc., a culture solution containing the culture and liquid medium is placed in a container, and the culture is allowed to proceed while stirring the culture solution. For example, Patent Documents 1 to 3 describe connecting a port or tube to the culture container in order to sample the culture solution.

JP 2012-120495 A JP 2016-136928 A JP 2016-202089 A

When sampling a portion of the culture medium from a port or tube, the culture medium may be extracted from a sealing member such as a septum or rubber stopper using a syringe or the like. However, if the sealing member becomes deformed, deteriorates, cores, etc., there is a risk that the culture medium may become contaminated by the intrusion of outside air or foreign matter, or that it may become impossible to repeatedly sample the culture medium.

The present invention was made in consideration of the above circumstances, and aims to provide a sampling device, a culture device, and a method for producing a culture that can easily collect liquid from a container.

One aspect of the present invention is a sampling device that collects a portion of a liquid contained in a container, comprising: a sampling flow path that circulates the liquid toward a recovery section that collects a portion of the liquid collected from the container; a cleaning liquid supply section that supplies cleaning liquid to a washable section that is set in at least a portion of the sampling flow path; and a cleaning liquid discharge section that discharges the cleaning liquid from the washable section, wherein the flow of the liquid from the container to the washable section and the flow of the cleaning liquid from the cleaning liquid supply section to the washable section are alternatively selectable, and the flow of the liquid from the washable section to the recovery section and the flow of the cleaning liquid from the washable section to the cleaning liquid discharge section are alternatively selectable.

A one-way valve may be disposed in the sampling flow path between the container and the cleanable section, preventing flow from the cleanable section to the container.
A liquid delivery device for the liquid may be disposed in the sampling flow path, and a liquid delivery device for the cleaning liquid may be disposed in the cleaning liquid supply section or the cleaning liquid discharge section.
The liquid delivery device or the cleaning liquid delivery device may be capable of arbitrarily setting the amount of liquid to be delivered.
An ultraviolet irradiation device for sterilizing the sampling flow path may be disposed between the container and the washable section.

Another aspect of the present invention is a culture apparatus comprising the sampling device and a culture vessel connected to the sampling flow path as the vessel.
Moreover, one aspect of the present invention is a method for producing a culture, comprising: a culturing step of supplying a liquid medium to a culture vessel and culturing a culture; a sampling step of using the sampling device to collect a portion of the culture liquid contained in the culture vessel; and a cleaning step of using the sampling device to supply the cleaning liquid to the cleanable section and clean the cleanable section.

According to the present invention, the sampling flow path connected to the container can be cleaned with a cleaning solution, and contamination of the container can be suppressed, making sampling easy.

FIG. 2 is a configuration diagram illustrating a sampling process of the sampling device of the embodiment. 1 is a configuration diagram illustrating a cleaning process of the sampling device of the embodiment. FIG.

The present invention will be described below based on a preferred embodiment with reference to the drawings.

Figures 1 and 2 show a sampling device 100 according to an embodiment. This sampling device 100 collects a portion of liquid 11 contained in a container 12 in a liquid treatment device 10 such as a culture device. This liquid treatment device 10 includes a container 12 that contains the liquid 11, and a container installation section 13 in which the container 12 is installed. The container 12 can be used as an operation container (liquid treatment container) for the liquid 11 in an operation process such as culture or chemical reaction.

Examples of the liquid 11 include various liquids such as aqueous liquids, oil-based liquids, solutions, electrolytes, dispersions, and emulsions. The liquid 11 may contain air bubbles, particles, powders, granular matter, cells, microorganisms, and the like. The container 12 may be open to the outside air, but is preferably sealed from the outside air. When the container 12 is a culture container, the liquid 11 contains, for example, a culture liquid obtained by culturing a culture of cells, microorganisms, or the like in a liquid medium. Within the container 12, gas may be present in the head space, or gas may be dissolved in the liquid 11.

The capacity (size) of the container 12 is not particularly limited, but can be, for example, 0.1 L to 5000 L. The container 12 may be single-use (disposable). The container installation unit 13 may be a support stand that supports the container 12, and may have functions such as a rocking device that rocks the container 12 and a weighing scale that measures the weight of the container 12. The container 12 may be constructed from a film-like container such as a resin, and the container 12 may be housed in a hard outer container. In this case, the outer container housing the container 12 may be installed on the container installation unit 13.

The sampling device 100 has a sampling section 20 for collecting a portion of the liquid 11. The sampling section 20 has a sampling flow path 21 for circulating the liquid 11 toward a recovery section 30 that recovers a portion of the liquid 11 collected from the container 12. The container 12 and the sampling flow path 21 are connected using a connection port 14. When the liquid processing device 10 is a culture device or the like, it is preferable that the connection port 14 is configured from a sterile connection port, a sterile connection connector, or the like that allows for a sterile connection.

A cleanable section 21B is set in at least a portion of the sampling flow path 21. The cleanable section 21B may be set in one location of the sampling flow path 21 extending from the container 12 to the collection section 30, or in two or more locations. In the description of the embodiment, the section of the sampling flow path 21 from the container 12 to the cleanable section 21B may be referred to as the container side section 21A, and the section from the cleanable section 21B to the collection section 30 may be referred to as the collection section side section 21C.

The recovery unit 30 can recover the liquid 11 that has flowed through the sampling flow path 21 into the recovery container 33. The configuration of the recovery unit 30 is not particularly limited, but a nozzle 31 may be connected to the end of the recovery unit side section 21C of the sampling flow path 21, and the collected liquid 11 may be ejected from the nozzle 31 into the recovery container 33. In this case, since the beginning of the liquid 11 ejected from the nozzle 31 may be affected when flowing through the sampling flow path 21, the liquid 11 may be discarded in the waste liquid container 32 instead of being collected in the recovery container 33. By collecting the liquid 11 ejected from the nozzle 31 into the recovery container 33 after discarding an appropriate amount of liquid 11, the influence of the liquid 11 due to collection can be suppressed.

After a predetermined amount of liquid 11 is collected in the collection container 33, the liquid 11 discharged from the nozzle 31 may be disposed in the waste liquid container 32. It is preferable that the support base 35 is switchable between the waste liquid container 32 and the collection container 33 being placed under the nozzle 31. When the collection container 33 is a test tube, an ampule, a beaker, or the like, a holder 34 may be placed on the support base 35, and the collection container 33 may be housed in the holder 34. Two or more collection containers 33 may be placed on the holder 34 or support base 35, and the liquid 11 discharged from the nozzle 31 may be received in turn in the two or more collection containers 33.

In the collection section 30, instead of providing a nozzle 31, the sampling flow path 21 and the collection container 33 may be directly connected. Also, a branching section (not shown) may be provided at the end of the collection section side section 21C of the sampling flow path 21, and the waste liquid container 32 and the collection container 33 may be connected to this branching section in a switchable manner. The collection container 33 may be a device having a movable part, such as a syringe or an injector.

The sampling device 100 includes a cleaning liquid supply section 40 that supplies cleaning liquid 41 to the cleanable section 21B, and a cleaning liquid discharge section 50 that discharges cleaning liquid 41 from the cleanable section 21B. The cleaning process of supplying cleaning liquid 41 to the cleanable section 21B and discharging waste liquid 51 of the cleaning liquid 41 to the cleaning liquid discharge section 50 can be performed before the sampling process, after the sampling process, or both before and after the sampling process.

The cleaning liquid supply unit 40 includes a cleaning liquid supply container 42 that contains cleaning liquid 41, and a cleaning liquid supply flow path 43 that circulates the cleaning liquid 41 from the cleaning liquid supply container 42 toward the cleanable section 21B. The cleaning liquid 41 is used to wash away liquid 11 and the like that may remain in the cleanable section 21B during sampling.

The cleaning liquid 41 preferably contains a solvent such as water or an organic solvent that dissolves the liquid 11 or components such as its solutes. The cleaning liquid 41 may also contain a cleaning agent, a surfactant, a decomposing agent, a disinfectant, etc. When the liquid 11 is a culture liquid, it is preferable that the influence of the cleaning liquid 41 components remaining in the washable section 21B on the liquid 11 flowing through the sampling flow path 21 is suppressed. For example, a buffer solution, physiological saline, etc. are suitable as the cleaning liquid 41 for the culture liquid. Two or more cleaning liquid supply units 40 may be connected to the sampling flow path 21, and two or more types of cleaning liquid 41 may be used to clean the washable section 21B.

The cleaning liquid supply container 42 and the cleaning liquid supply flow path 43 are connected using a connection port 46. When the liquid 11 flowing through the sampling flow path 21 is a culture liquid or the like, it is preferable to configure the connection port 46 from a sterile connection port, a sterile connection connector, or the like that allows for a sterile connection. The cleaning liquid supply container 42 may have a cleaning liquid supply port 47 for replenishing the cleaning liquid 41.

The cleaning liquid discharge section 50 includes a cleaning liquid discharge container 52 that stores waste liquid 51 of the cleaning liquid 41 discharged from the cleanable section 21B, and a cleaning liquid discharge flow path 53 that distributes the waste liquid 51 of the cleaning liquid 41 from the cleanable section 21B toward the cleaning liquid discharge container 52. The waste liquid 51 of the cleaning liquid 41 is the cleaning liquid 41 that has passed through the cleanable section 21B. The waste liquid 51 of the cleaning liquid 41 may also include foreign matter removed from the cleanable section 21B. For this reason, in the following description, the cleaning liquid 41 that has passed through the cleanable section 21B may be simply referred to as the cleaning liquid 41 or waste liquid 51.

The cleaning liquid discharge flow path 53 may be provided with a flow meter 56 for checking the flow rate of the waste liquid 51. The cleaning liquid discharge container 52 may have a waste liquid port 57 for removing the waste liquid 51. By controlling the amount of cleaning liquid 41 circulating through the cleaning liquid discharge flow path 53 and the amount of waste liquid 51 contained in the cleaning liquid discharge container 52, it is possible to prevent the waste liquid 51 from overflowing from the cleaning liquid discharge container 52 and flowing back into the sampling flow path 21.

In the embodiment of the sampling device 100, the flow of the liquid 11 from the container 12 to the washable section 21B and the flow of the cleaning liquid 41 from the cleaning liquid supply unit 40 to the washable section 21B are configured to be alternatively selectable. For example, the valve 22 arranged in the container side section 21A of the sampling flow path 21 and the valves 44, 45 arranged in the cleaning liquid supply flow path 43 may be switched and controlled so that the valve 22 and the valves 44, 45 are not opened at the same time. The valve 22 in the container side section 21A and the valve 44 in the cleaning liquid supply flow path 43 may be configured separately, or a valve V1 that can be linked together may be configured. In addition, a flow path switching device such as a three-way valve having three or more inlets and outlets and having the functions of the valves 22 and 44 may be arranged at the position where the cleaning liquid supply flow path 43 joins the sampling flow path 21.

In addition, in the embodiment of the sampling device 100, the flow of the liquid 11 from the washable section 21B to the collection section 30 and the flow of the cleaning liquid 41 from the washable section 21B to the cleaning liquid discharge section 50 are configured to be alternatively selectable. For example, the valves 23, 24 arranged in the collection section 21C of the sampling flow path 21 and the valve 54 arranged in the cleaning liquid discharge flow path 53 may be switched and controlled so that the valves 23, 24 and the valve 54 are not opened at the same time. The valve 23 in the collection section 21C and the valve 54 in the cleaning liquid discharge flow path 53 may be configured separately, or a valve V2 that can be linked together may be configured. In addition, a flow path switching device such as a three-way valve having three or more inlets and outlets and having the functions of the valves 23, 54 may be arranged at the position where the cleaning liquid discharge flow path 53 branches off from the sampling flow path 21.

As shown in FIG. 1, in the sampling process, valves 22, 23, and 24 arranged in the container side section 21A and the recovery section 21C of the sampling flow path 21 are simultaneously open, while valve 44 arranged in the cleaning liquid supply flow path 43 and valve 54 arranged in the cleaning liquid discharge flow path 53 are closed. In this case, liquid 11 can be circulated from the container 12 toward the recovery section 30 by operating a liquid delivery device 25 such as a pump.

The liquid 11 delivery device 25 can be placed in at least one location of the sampling flow path 21, for example, in the container side section 21A, the cleanable section 21B, or the recovery section 21C. If the liquid delivery device 25 is not placed in the cleanable section 21B, but is placed in the container side section 21A or the recovery section 21C, cleaning of the liquid delivery device 25 becomes unnecessary when cleaning the cleanable section 21B. It is preferable that the liquid delivery device 25 for liquid 11 can arbitrarily set the amount of liquid to be delivered. This makes it easy to automate the sampling process of liquid 11.

As shown in FIG. 2, in the cleaning process, the valves 44, 45, and 54 arranged in the cleaning liquid supply flow path 43 and the cleaning liquid discharge flow path 53 are simultaneously open, while the valve 22 arranged in the container side section 21A of the sampling flow path 21 and the valve 23 arranged in the recovery section 21C are closed. In this case, the cleaning liquid 41 can be circulated from the cleaning liquid supply container 42 to the cleaning liquid discharge container 52 by operating a liquid delivery device 55 such as a pump.

The liquid delivery device 55 for the cleaning liquid 41 can be placed in at least one of the cleaning liquid supply section 40, the cleanable section 21B, or the cleaning liquid discharge section 50. If the liquid delivery device 55 is not placed in the cleanable section 21B, but is placed in the cleaning liquid supply section 40 or the cleaning liquid discharge section 50, cleaning of the liquid delivery device 55 becomes unnecessary when cleaning the cleanable section 21B. It is preferable that the liquid delivery amount of the liquid delivery device 55 for the cleaning liquid 41 can be set arbitrarily. This makes it possible to easily automate the cleaning process of the sampling flow path 21.

According to the embodiment of the sampling device 100, by connecting the cleaning liquid supply unit 40 and the cleaning liquid discharge unit 50 to the sampling flow path 21, the sampling flow path 21 can be cleaned with the cleaning liquid 41. This makes it possible to keep the sampling flow path 21 clean, making it easy to perform sampling. In addition, the flow of the liquid 11 from the container 12 to the cleanable section 21B can be switched by the valve 22, thereby preventing contamination of the container 12.

The proportion of the length of the sampling flow path 21 that is occupied by the washable section 21B is not particularly limited, but it is preferable that the length of the washable section 21B is longer than the length of the container side section 21A, the length of the collection section 21C, or the combined length of the container side section 21A and the collection section 21C. For example, the washable section 21B may occupy more than half of the length of the sampling flow path 21.

Contamination of the container 12 can be caused in part by the liquid 11, cleaning solution 41, waste liquid 51, etc. in the sampling flow path 21 flowing back into the container 12 via the sampling flow path 21. For this reason, it is preferable to place a one-way valve 26 in the container side section 21A of the sampling flow path 21 to block flow from the cleanable section 21B to the container 12. The one-way valve 26 has a higher ability to block backflow than the valve 24, which switches between on and off, and therefore can more reliably prevent contamination of the container 12.

A device for sterilizing the inside of the sampling flow path 21 may be placed in the container side section 21A of the sampling flow path 21. For example, an ultraviolet irradiation device 27 that irradiates ultraviolet (UV) rays can locally sterilize the inside of the sampling flow path 21 from outside the sampling flow path 21 without using high temperatures or chemicals, and without the ultraviolet rays affecting the liquid 11 in the container 12. For example, even if the automatic control of the sampling device 100 is affected due to a temporary power outage, contamination of the liquid 11 in the container 12 can be suppressed by operating the ultraviolet irradiation device 27. When the ultraviolet irradiation device 27 is used, at least the section of the sampling flow path 21 where the ultraviolet rays are irradiated is made of a flow path made of a material that is transparent to ultraviolet rays.

An optical sensor 28 may be disposed in the cleanable section 21B of the sampling flow path 21. The optical sensor 28 can be used to inspect the inside of the sampling flow path 21 from outside the sampling flow path 21, thereby checking the condition of the cleanable section 21B. The wavelength (type) of inspection light used by the optical sensor 28 is not limited to visible light, and may be infrared light, ultraviolet light, etc. When the optical sensor 28 is used, a flow path made of a material that is transparent to the inspection light is used in at least the section of the sampling flow path 21 where the inspection light is irradiated.

The cleaning liquid supply flow path 43 may have a branch section 43A where the flow path branches. A valve 48, a port 49, etc. may be arranged in the branch section 43A. For example, when an excess of cleaning liquid 41 is accumulated in the cleaning liquid supply flow path 43, the valve 48 of the branch section 43A can be opened and unnecessary cleaning liquid 41 can be drained from the port 49 while the valves 44, 45 provided before and after the point where the branch section 43A branches from the cleaning liquid supply flow path 43 are closed. The valves 45, 48 may be configured separately, or may be configured as an integrated valve V3.

When valves 44, 45 are arranged in two or more locations between the cleaning liquid supply container 42 and the cleanable section 21B of the cleaning liquid supply flow path 43, it is preferable to close at least the valve 44 closest to the cleanable section 21B during the sampling process. This makes it possible to suppress the amount of sampling liquid 11 entering the cleaning liquid supply flow path 43 and make it easier to control sampling. The same applies when valves 54 are arranged in two or more locations of the cleaning liquid discharge flow path 53.

The configuration of the flow paths such as the sampling flow path 21, the cleaning liquid supply flow path 43, and the cleaning liquid discharge flow path 53 is not particularly limited, and may be a flexible tube, a hose, or a hard pipe. Examples of materials for these flow paths include resin, rubber, elastomer, and metal. Each flow path may be provided with a filter, a flow monitor, a flow meter, and the like.

The configuration of the valves 22, 23, 24, 44, 45, 48, and 54 provided in the flow path is not particularly limited, but can be appropriately selected from the viewpoints of operability, reliability, convenience, etc. For flexible tubes, hoses, etc., it is preferable to use pinch valves that close the flow path by compressing the tubes, hoses, etc. from the outside, as this can suppress the influence and contamination of the contents of the flow path. For the same reason, it is preferable to use tube pumps as the liquid delivery devices 25 and 55, which apply power to the contents of the flow path by compressing the tubes, hoses, etc. from the outside.

The configuration of the containers such as the container 12, the cleaning liquid supply container 42, and the cleaning liquid discharge container 52 is not particularly limited, and may be a flexible container such as a film-like container, or a hard container. Examples of film-like containers include a flat bag in which two flat films are stacked together and sealed at the periphery, a gusset bag in which a folded gusset portion is joined and sealed between two flat films, and a cylindrical film container in which a flat film is joined and sealed to at least one opening of a cylindrical film with both ends open.

The cylindrical film container may have a structure in which circular flat films are joined to both ends of a cylindrical film, like a drum can liner. When a flat film is joined to only one opening of the cylindrical film, the cylindrical film may be crushed in the radial direction at the opening on the opposite side, and the inner surfaces of the cylindrical film may be directly joined together. The cylindrical film container is not limited to a cylindrical shape, and may be configured as a square tube whose cross-sectional shape on the horizontal plane is a polygon such as a square. It is preferable to configure the container with multiple bags, such as double bags or triple bags, because this makes it less likely for the contents of the container (liquid 11, cleaning liquid 41, waste liquid 51, etc.) to leak.

The material of the film constituting the film-like container is not particularly limited, but examples include thermoplastic resins such as polyamide, polyester, polyolefin, and acrylic, and laminates thereof. These thermoplastic resins may have an aliphatic or aromatic polymer structure, or may have a cyclic or non-cyclic structure. When the film-like container is mainly made of a highly versatile thermoplastic resin such as polyethylene (PE), polypropylene (PP), or polyethylene terephthalate (PET), it is preferable because even large containers can be manufactured relatively inexpensively and it is easy to meet the demand for single use.

When connecting accessories such as tubes, hoses, injection ports, and injection ports as various types of pipelines to the film-like container, it is preferable that the material of the accessories is an organic material such as resin, rubber, or elastomer that can be heat-sealed to the film-like container. This makes it easy to connect the accessories to the film-like container by heat sealing. It also makes it easy to configure the film-like container, including the accessories, for single use.

When a flexible container is housed in a rigid outer container, it is preferable that the outer container has a hardness (rigidity) that can mechanically protect the flexible container. The material of the outer container is not particularly limited, and it can be made of resin, rubber, glass, metal, wood, fiber-reinforced plastic, etc. By protecting the periphery of the flexible container with the outer container, the shape of the flexible container can be easily maintained. The side of the container containing the contents may be in close contact with the inner surface of the outer container, and the weight of the container may be supported by the outer container. The outer container may be equipped with a heater, a cooler, a radiator, a heat exchanger that circulates a heat medium, a thermometer, etc. to adjust the temperature of the contents housed in the container.

In the embodiment of the sampling device 100, when the container 12 is a culture container, the liquid treatment device 10 can be used as a culture device. The culture device supplies a liquid medium to the container 12 to culture a culture. When the liquid medium is supplied to the container 12 and a culture of microorganisms, cells, etc. is cultured, the liquid 11 contained in the container 12 becomes the culture liquid. The culture liquid may be any liquid used in the culture process. Depending on the progress of the culture process, the state of the culture liquid may be in various states, such as a state in which the liquid medium is the main component, a state in which the culture is seeded in the liquid medium, a state in which the culture has grown in the liquid medium, and a state in which products and waste products of the culture are secreted.

In addition, in the culture process in which a liquid medium is supplied to a culture vessel to culture a culture, when a sampling process is performed, a portion of the culture liquid contained in the culture vessel can be collected using the sampling device 100. The state of the culture liquid can be confirmed by inspecting, analyzing, etc. the sampled culture liquid. In addition, as described above, by performing a cleaning process before or after the sampling process, the sampling flow path 21 can be kept clean and the reliability of sampling can be maintained. In particular, when the culture process is performed on a large scale, the state of the large amount of culture liquid contained in the culture vessel can be accurately grasped through sampling of the culture liquid, making it easier to deal with the situation according to the circumstances.

In the culture process, in order to stir the contents of the culture vessel, a stirring member such as a stirring blade, stirring rod, magnetic stirrer, etc. may be placed inside the culture vessel, or the culture vessel may be rocked from outside the culture vessel using a rocking device. When a rocking device is used, the culture liquid can be stirred without the rocking device coming into contact with the culture liquid. Compared to using a stirring member, damage to the stirring member, contamination of the culture liquid due to deterioration, etc., and the trouble of replacing the stirring member can be avoided.

The rocking device may rock the liquid 11 by reciprocating the container 12. The rocking device may rock the liquid 11 by rotating the container 12. The direction of the reciprocating or rotational motion may be horizontal (left and right), vertical (up and down), or an inclined direction between these. The rotational motion may be a rotational motion in which the rotation axis passes through the center of gravity of the liquid 11, or a revolution motion in which the rotation axis passes through a position away from the center of gravity of the liquid 11. The rocking device may simultaneously superimpose two or more types of motion with different types or directions and apply them to the container 12. The rocking device may also change the type or direction of motion periodically or irregularly with respect to the time axis.

The cultures of microorganisms, cells, etc. cultured in the culture process are not particularly limited, but include fungi, bacteria, viruses, yeast, algae, insect cells, plant cells, animal cells, CHO (Chinese Hamster Ovary) cells for biopharmaceutical production, HeLa cells, COS cells, iPS cells for regenerative medicine applications, stem cells including mesenchymal stem cells, and animal cells such as differentiated tissue cells. In addition, in the culture process, it is not limited to obtaining cultures of microorganisms, cells, etc. as the target product, but it is also possible to recover products such as enzymes, antibodies, and chemical substances produced by the cultures from the culture solution.

The present invention has been described above based on a preferred embodiment, but the present invention is not limited to the above embodiment, and various modifications are possible without departing from the gist of the present invention.

In the above embodiment, the sampling flow path 21 connects one container 12 and one collection section 30, but the present invention is not limited to this. For example, the container side section 21A of the sampling flow path 21 may be branched into two or more sections, and two or more containers 12 may be connected to the sampling flow path 21. Also, the collection section side section 21C of the sampling flow path 21 may be branched into two or more sections, and two or more collection sections 30 may be connected to the sampling flow path 21.

For example, when culturing the same type of culture using multiple containers 12, the components used to recover the collected culture fluid (recovery section 30, recovery section side section 21C, etc.) and the components used to clean the sampling flow path 21 (cleaning liquid supply section 40, cleanable section 21B, cleaning liquid discharge section 50, etc.) can be standardized to reduce space, costs, etc.

10...liquid treatment device, 11...liquid, 12...container, 20...sampling section, 21...sampling flow path, 21B...cleanable section, 25...liquid delivery device, 26...one-way valve, 27...ultraviolet irradiator, 30...recovery section, 40...cleaning liquid supply section, 41...cleaning liquid, 42...cleaning liquid supply container, 43...cleaning liquid supply flow path, 50...cleaning liquid discharge section, 51...cleaning liquid waste, 52...cleaning liquid discharge container, 53...cleaning liquid discharge flow path, 55...cleaning liquid delivery device, 100...sampling device.

Claims (5)

A sampling device for sampling a portion of a liquid contained in a container, comprising:
a sampling flow path that causes the liquid to flow toward a collection unit that collects a portion of the liquid collected from the container;
a cleaning liquid supply unit that supplies a cleaning liquid to a cleanable section that is set in at least a portion of the sampling flow path;
a cleaning liquid discharge section that discharges the cleaning liquid from the cleanable section;
Equipped with
The sampling flow path has a container side section between the container and the washable section,
the sampling flow path has a one-way valve in the container side section that blocks flow from the washable section to the container;
an ultraviolet irradiator for sterilizing the sampling flow path is disposed on the container side of the one-way valve in the sampling flow path,
The flow of the liquid from the container to the washable section and the flow of the cleaning liquid from the cleaning liquid supply unit to the washable section can be alternatively selected, and
A sampling device characterized in that the flow of the liquid from the cleanable section to the recovery section and the flow of the cleaning liquid from the cleanable section to the cleaning liquid discharge section can be alternatively selected. The sampling device according to claim 1, wherein a liquid delivery device for the liquid is disposed in the sampling flow path, and a liquid delivery device for the cleaning liquid is disposed in the cleaning liquid supply section or the cleaning liquid discharge section. 3. The sampling device according to claim 2 , wherein the liquid delivery device or the cleaning liquid delivery device is capable of arbitrarily setting the amount of liquid to be delivered. A sampling device according to any one of claims 1 to 3 ;
A culture vessel connected to the sampling flow path as the vessel;
A culture device comprising: A culturing step of culturing a culture by supplying a liquid medium to the culture vessel using the culture apparatus according to claim 4 ;
A sampling step of collecting a portion of the culture solution contained in the culture vessel using the sampling device;
a cleaning step of supplying the cleaning liquid to the cleanable section by using the sampling device to clean the cleanable section;
A method for producing a culture, comprising the steps of:

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