JP7336757B2 - Filter for phototherapy equipment - Google Patents

Description

The present invention relates to a phototherapeutic device filter used in a phototherapeutic device that treats skin diseases by irradiating ultraviolet rays.

BACKGROUND ART In recent years, a method of irradiating an affected area with ultraviolet rays has been widely used as a method for treating skin diseases. As a phototherapeutic device for skin diseases that treats skin diseases with ultraviolet rays, a device that includes a discharge lamp as an ultraviolet light source and emits ultraviolet rays (UVA1) in the wavelength range of 340 to 400 nm has been put into practical use (specifically, for example "SELLAMED 2000 System Dr. Sellmeier"). A certain type of phototherapeutic device for skin diseases that has been put into practical use is configured to irradiate the affected area with light from a metal halide lamp through a plurality of (specifically, for example, three) wavelength selection filters. Therefore, there is a problem that the utilization efficiency of the light (ultraviolet rays) from the ultraviolet light source is low.

Therefore, it has been proposed to use an LED element as an ultraviolet light source in a skin disease phototherapy device that treats a skin disease with ultraviolet light (see, for example, Patent Documents 1 and 2).
The phototherapy device for skin disease according to Patent Document 1 has a plurality of LED elements arranged on a circular substrate or on the concave surface of a housing having a concave surface. Further, in Patent Document 2, an LED element having a peak wavelength in the wavelength range of 350 to 390 nm is used for intractable eczema, dyshidrotic eczema, cutaneous T-cell lymphoma, atopic dermatitis, alopecia areata, keloid, scar, There is a description that it is effective for the treatment of skin atrophic linear and scleroderma.

On the other hand, long-wavelength ultraviolet rays with a wavelength of 320 to 400 nm are known to have an immediate darkening effect of darkening the skin immediately after irradiation. Non-Patent Document 1 shows a spectrum of action on the skin, and discloses that a wavelength of 340 nm is the peak of the immediate darkening action. Immediate darkening action is that the skin darkens immediately after irradiation with long-wavelength ultraviolet rays, and then returns to its original state in several hours or several days. However, repeated immediate tanning may result in persistent long-term hyperpigmentation.

JP-A-10-190058 JP 2007-151807 A

C.IRWIN, A. BARNES, D.VERES, K.KAIDBEY, "An ultraviolet radiation action spectrum for immediate pigment darkening", Photochemistry and Photobiology, Vol.57, No.3, pp.504-507, 1993

The inventors of the present invention conducted extensive research and found that an LED device with a peak wavelength of radiated light of 365 nm is most effective as a light source for use in a phototherapeutic device for scleroderma. Therefore, by using an LED element that emits light with a peak wavelength of 365 nm as a light source, an excellent and efficient therapeutic effect for scleroderma can be obtained.
Skin diseases to which ultraviolet light therapy is applied range from tens of cm ² to hundreds of cm ² . Therefore, in the case of a phototherapy device using an LED light source, it is necessary to mount a plurality of LED elements.

However, even if a plurality of LED elements manufactured aiming at a peak wavelength of 365 nm are used as the LED light source, the peak wavelength of the light emitted from the LED light source has a variation of about ±5 nm due to manufacturing variations of the LED elements. can occur. At that time, if the peak wavelength shifts to the short wavelength side with respect to 365 nm, the radiation amount of light around 340 nm increases, so an immediate blackening reaction is likely to occur. For example, for scleroderma, repeated irradiation with long-wavelength ultraviolet rays is assumed as a symptomatic treatment, so there is a high possibility that immediate blackening will be repeated and long-term pigmentation will occur. This pigmentation increases the absorption of light in the epidermis and reduces the penetration depth of the light into the targeted dermis, thus reducing treatment efficiency.
In order to obtain a phototherapeutic device capable of obtaining an excellent skin therapeutic effect while suppressing immediate blackening, the therapeutic light emitted from the phototherapeutic device has a wavelength at which the risk of immediate blackening increases is cut. must be bright light.
SUMMARY OF THE INVENTION Accordingly, it is an object of the present invention to provide a filter for a phototherapeutic device that can obtain excellent skin therapeutic effects while suppressing immediate blackening.

In order to solve the above problems, one aspect of the filter for a phototherapy device according to the present invention is a plurality of LED elements that emit light having a peak wavelength in a wavelength range of 365±5 nm and including light with a wavelength of 350 nm or less. is used as a light source to irradiate an affected area with therapeutic light, the filter for a phototherapy device is composed of a colored glass filter and has a first wavelength and a transmittance of 5%. is 350 nm or more and 365 nm or less, and has filter characteristics such that the interval between the first wavelength and the second wavelength is 30 nm or less, and the plurality of radiated light from the LED element is incident thereon , and transmitted light is emitted as the treatment light.

In this way, light with a wavelength of 355 nm or less is substantially blocked out of the light emitted from the plurality of LED elements, so even when the peak wavelength of the LED element falls within the range of 365±5 nm, the lower limit of variation is Even if there is, the light around 340 nm wavelength that causes the immediate darkening effect can be substantially removed from the treatment light, and the immediate darkening effect can be reduced. As a result, pigmentation due to repeated immediate darkening can be suppressed, and the deep penetration of light into the target dermis can be maintained. Therefore, by installing this filter for a phototherapeutic device, it is possible to obtain a phototherapeutic device capable of obtaining an excellent skin therapeutic effect for scleroderma while suppressing immediate blackening.

In particular, light with a wavelength of 355 nm or less is substantially removed from the therapeutic light among the light emitted from the plurality of LED elements, so that the therapeutic effect can be higher than the side effect (immediate blackening).

Furthermore, the filter for the phototherapeutic device described above may be composed of a colored glass filter.
In this case, the light utilization efficiency can be improved compared to, for example, the case where the phototherapeutic filter is a dielectric multilayer filter. In addition, since the dielectric multilayer filter depends on the incident angle, in order to improve the light utilization efficiency, the dielectric multilayer filter should be used after improving the angular characteristics of the light from the LED using a collimating lens. must be used, which complicates the filter configuration and increases the size. By configuring the filter for the phototherapeutic apparatus with a colored glass filter, the filter configuration can be simplified and the size of the filter can be reduced.

Further, in the above filter for a phototherapeutic device, the midpoint wavelength between the first wavelength at which the transmittance is 5% and the second wavelength at which the transmittance is 72% is 350 nm or more and 365 nm or less; It may have filter characteristics such that the distance between the one wavelength and the second wavelength is 30 nm or less. In this case, wavelengths (unnecessary wavelengths) that increase the risk of immediate blackening can be cut, while wavelengths that increase the therapeutic effect (effective wavelengths) can be transmitted.
Furthermore, the filter for phototherapy apparatus may have a filter characteristic that the first wavelength is 340 nm or less. In this case, the effective wavelength can be properly transmitted.
Further, the filter for phototherapy apparatus may have filter characteristics such that the average value of transmittance in the wavelength range from the second wavelength to 800 nm is 80% or more. In this case, the effective wavelength can be extracted efficiently.

ADVANTAGE OF THE INVENTION According to this invention, it can be set as the phototherapeutic apparatus which can acquire the outstanding skin therapeutic effect, suppressing immediate blackening.

BRIEF DESCRIPTION OF THE DRAWINGS It is a whole block diagram which shows an example of the phototherapeutic apparatus of this embodiment. It is a figure which shows the concrete structure of a light source part. It is a figure which shows the mechanism of immediate blackening. FIG. 4 is a diagram showing pigmentation due to repeated irradiation; Fig. 10 shows the action spectrum of light sources, filter characteristics and instant blackening; It is a figure explaining the transmission characteristic of a filter. It is a figure which shows the spectrum after filter transmission. FIG. 10 is a diagram showing the results of Experiment 1; FIG. 10 is a diagram showing the results of Experiment 2; It is a figure showing an effect|action when pigmentation occurs. It is a figure which shows another structure of a light source part.

BEST MODE FOR CARRYING OUT THE INVENTION An embodiment of the present invention will be described below with reference to the drawings.
FIG. 1 is an overall configuration diagram showing an example of a phototherapy device 10 according to this embodiment.
This phototherapy device 10 is a treatment device for skin diseases that performs UVA1 therapy by irradiating therapeutic light having a spectrum in the UVA1 region to an affected area.
UVA1 therapy is a treatment method that uses ultraviolet rays of 340 nm to 400 nm, and is characterized in that it reaches deeper into the skin than treatment methods that use ultraviolet rays in the UV-B region. Therefore, it is known to be effective against diseases having a etiology in the dermis, and highly effective against atopy, prurigo, and scleroderma.

In this embodiment, a case where the phototherapy device 10 is a treatment device for treating scleroderma will be described.
The phototherapy device 10 includes a light source section 20 and a control section 30 that controls the light source section 20 . The light source section 20 and the control section 30 are supported by the support 11 .
The support 11 includes a pedestal 12 supported on the floor surface via wheels 18, a support 13 extending upward at the central portion of the pedestal 12, and a light source section 20 attached to the support 13 at the upper end of the support 13. and an actuating arm 14 that is pivotably supported. In the support 11 , the light source section 20 is attached to the distal end of the operating arm 14 . Also, the control unit 30 is attached to the central portion of the post 13 by a fixing member (not shown).

The light source unit 20 includes, for example, a rectangular parallelepiped housing 27 and a window member 28 provided on an end face of the housing 27 , and radiates therapeutic light from the window member 28 . This window member 28 is a light irradiation window for irradiating therapeutic light onto a diseased part of a patient. The light source unit 20 may be provided with a manual lever 19 for manually swinging the light source unit 20 .
The control unit 30 includes, for example, a rectangular parallelepiped housing 37 and a graphic operation panel 39 provided on the side surface of the housing 37 . The graphic operation panel 39 can be operated by an operator (eg, doctor) of the phototherapy device 10 .

FIG. 2 is a diagram showing a specific configuration of the light source section 20. As shown in FIG.
As shown in FIG. 2, the light source section 20 includes a light source unit 21 arranged in a housing section 27a formed inside a housing 27. As shown in FIG. The light source unit 21 is supported by a support member (not shown) in the housing portion 27 a and arranged to face the light irradiation window 28 . Here, the light irradiation window 28 is arranged so as to close the opening 29 of the housing portion 27a.

The light source unit 21 includes a plurality of LED elements 24 as light sources. The plurality of LED elements 24 are arranged on, for example, a rectangular plate-like substrate 23 inside a rectangular tubular frame 25, for example. For example, the plurality of LED elements 24 can be arranged on the substrate 23 in a grid pattern arranged vertically and horizontally at predetermined intervals. For example, when 64 LED elements 24 are used, the LED elements 24 can be arranged in a matrix of 8×8. Note that the number and arrangement of the LED elements 24 are not limited to the above.
The distance between the substrate 23 and the light irradiation window 28 can be set to 40 mm, for example.
Further, the light source section 20 may include a heat sink 22a and an axial fan 22b for cooling the light source unit 21 on the opposite side of the light irradiation window 28 of the light source unit 21 in the housing section 27a.

Further, the light source unit 20 may include an irradiation attachment 25 outside the light irradiation window 28 so as to block the opening 29 of the housing portion 27a. By providing the irradiation attachment 25, it is possible to prevent the affected part from directly touching the light irradiation window 28. - 特許庁For example, the thickness of the irradiation attachment 25 can be set to 10 mm, and the light irradiation section 28 can be arranged at a position 5 mm from the irradiation attachment 25 inside the housing section 27a.
Furthermore, the light source section 20 may include a reflecting member 27b that reflects the light emitted from the LED element 24 on the inner surface of the housing section 27a. Note that the inner surface of the housing portion 27a may be provided with a reflective property by subjecting the inner surface of the housing portion 27a to a mirror finish or the like.

Each of the plurality of LED elements 24 has a peak wavelength in a wavelength range of 365±5 nm (hereinafter also referred to as a “specific wavelength range”) and emits ultraviolet light including light with a wavelength of 350 nm or less. Specifically, the plurality of LED elements 24 has a peak wavelength in a specific wavelength range and emits ultraviolet rays (UVA1) in a wavelength range of 340 nm to 400 nm. Further, the half-value width (full width at half maximum) of the optical spectrum of the plurality of LED elements 24 can be 5 nm to 20 nm.

Ultraviolet light with a peak wavelength of 365 nm is the most effective light for treating scleroderma. The above specific wavelength range is a wavelength range that takes into consideration variations in the peak wavelength of LED elements manufactured aiming at a peak wavelength of 365 nm.
The peak wavelength of an LED element may vary by about ±5 nm due to manufacturing variations (variation in bandgap, etc.), unlike the emission line spectrum of a discharge lamp, for example. The specific wavelength range is set to a wavelength of 365 ± 5 nm because when using a plurality of LED elements manufactured aiming at a peak wavelength of 365 nm as a light source, the peak wavelength of each LED element may vary within the wavelength range of 365 ± 5 nm. This is foreseen.
As the LED element 24, for example, a surface-mounted LED element made of an AlInGaN-based semiconductor can be used.

A cable (not shown) is electrically connected to the light source unit 21 for supplying power to the plurality of LED elements 24 included in the light source unit 21 . This cable electrically connects the light source section 20 (light source unit 21 ) and the control section 30 .
A filter 26 is arranged between the plurality of LED elements 24 and the light irradiation window 28 in the housing portion 27a. The filter 26 substantially cuts (blocks) light with a wavelength of 350 nm or less, preferably 355 nm or less, out of the light emitted from the plurality of LED elements 24 . The filter 26 is a phototherapy device filter that is attached to the phototherapy device 10 (light source unit 20). The filter 26 may be configured to be detachable from the phototherapeutic device 10 (light source unit 20). In addition, since the plurality of LED elements 24 emit light in the UVA1 wavelength range (340 nm to 400 nm), the filter 26 substantially cuts light with a wavelength of 340 nm or more and 350 nm or less, preferably 340 nm or more and 355 nm or less. can be anything.

As described above, ultraviolet irradiation having a peak wavelength of 365 nm is most effective for treating scleroderma. In other words, the action spectrum of scleroderma treatment has a peak at a wavelength of 365 nm, and the therapeutic effect decreases with increasing distance from the wavelength of 365 nm. On the other hand, it is known that long-wavelength ultraviolet rays with a wavelength of 320 nm to 400 nm have an immediate darkening action of darkening the skin immediately after irradiation.
Here, the mechanism of immediate blackening will be described.
As shown in FIG. 3( a ), the structure of human skin 100 is such that the layer closest to the surface is the epidermis 110 , and the layer below it is the dermis 120 . pigment cells) serve to produce melanin-112. When irradiated with ultraviolet rays, as shown in FIG. 3(b), melanin 112 that has already been produced is photo-oxidized and blackened. After a certain period of time (several hours or days), the melanin darkened by photo-oxidation is reduced and returned to its original state, as shown in FIG. 3(c). This is how instant blackening works.
Further, when the ultraviolet irradiation is repeated and the immediate blackening is repeated, as shown in FIG. 4, the blackening of the melanin 112 accumulates and pigmentation occurs.
The action spectrum of the immediate blackening action has a peak at a wavelength of 340 nm as shown by curve S in FIG. 5, and ultraviolet irradiation with a wavelength of around 340 nm is most likely to cause immediate blackening.

In this embodiment, the LED element 24 is used as the light source of the phototherapy device 10 . The light spectrum of the light emitted from the LED element 24 is broad as shown by curves a to c in FIG. 5, and includes ultraviolet light with a wavelength of around 340 nm. That is, there is a risk of immediate blackening. In FIG. 5, curve a is the light spectrum of the LED element with a peak wavelength of 365 nm, curve b is the light spectrum of the LED element with a peak wavelength of 370 nm (wavelength 365 + 5 nm), and curve c is the peak wavelength of 360 nm (wavelength 365-5 nm). ) is the optical spectrum of the LED element.
Further, as described above, the peak wavelength of the LED element 24 has a variation of about ±5 nm. Since it approaches the wavelength of 340 nm, which is the peak of the spectrum, the risk of immediate blackening increases.

In order to reduce the risk of immediate blackening and obtain a therapeutic effect on scleroderma, among the light emitted from the plurality of LED elements 24, blocking of light near a wavelength of 365 nm, which is the peak of the therapeutic effect, is suppressed. , it is necessary to block light around a wavelength of 340 nm, which is the peak risk of immediate darkening.
In this embodiment, considering the balance between the therapeutic effect and the risk of immediate blackening, the filter 26 is used to substantially cut light with a wavelength of 350 nm or less among the light emitted from the plurality of LED elements 24. do. In other words, the light in the wavelength region where the risk of immediate blackening is greater than the therapeutic effect is cut. In addition, by substantially cutting light with a wavelength of 355 nm or less among the light emitted from the plurality of LED elements 24, it is possible to ensure that the therapeutic effect is higher than the risk of immediate blackening.

Filter 26 may be a colored glass filter. In this case, silicate glass, phosphate glass, or the like can be used as the material of the filter 26 .
FIG. 6 is a diagram explaining each parameter indicating the transmission characteristics of the colored glass filter.
The filter 26 in this embodiment can have filter characteristics such that the transmission limit wavelength (λT) is 350 nm or more and 365 nm or less and the wavelength tilt width (Δλ) is 30 nm or less. In addition, the filter 26 can have filter characteristics such that the transmittance (TH) in the high transmission range is 80% or more. Furthermore, the filter 26 can have filter characteristics such that the absorption limit wavelength (λ5) is 340 nm or less.

Here, as shown in FIG. 6, the transmission limit wavelength (λT) is the first wavelength (absorption limit wavelength (λ5)) at which the transmittance is 5%, and the second wavelength at which the transmittance is 72%. (High transmission limit wavelength (λ72)). Also, the wavelength tilt width (Δλ) is the interval between the first wavelength (absorption limit wavelength (λ5)) and the second wavelength (high transmission limit wavelength (λ72)). Furthermore, the transmittance (TH) in the high transmission range is the average value of the transmittances in the high transmission range (wavelength range from the high transmission limit wavelength (λ72) to 800 nm).

If the transmission limit wavelength (λT) is short, it is not possible to cut wavelengths (unnecessary wavelengths) that increase the risk of immediate blackening. Resulting in. Therefore, λT is preferably 350 nm or more and 365 nm or less. Also, if the wavelength tilt width (Δλ) is too long, the effective wavelength and the unnecessary wavelength cannot be separated, so Δλ is preferably 30 nm or less. Furthermore, if the transmittance (TH) of the high transmission region is too low, effective wavelengths cannot be extracted efficiently, so TH is preferably 80% or more. Also, if the absorption limit wavelength (λ5) is long, the effective wavelength is cut, so λ5 is preferably 340 nm or less.

Thus, the light source unit 20 has a plurality of LED elements 24 that emit light having a peak wavelength in the wavelength range of 365±5 nm, and the filter 26 receives the emitted light from the plurality of LED elements 24, Of the radiated light, light with a wavelength of 350 nm or less, preferably 355 nm or less is substantially cut, and transmitted light is emitted as therapeutic light. Then, therapeutic light, which is light transmitted through the filter 26 , is emitted from the light irradiation window 28 .
Here, as the light irradiation window 28, it is preferable to use a material having high mechanical strength as well as having optical transparency with respect to the therapeutic light. A specific example of the material of the light irradiation window 28 is, for example, quartz glass. By using silica glass as the material of the light irradiation window 28, it is possible to provide high mechanical strength and prevent breakage due to impact. In addition, when the light irradiation window 28 becomes dirty, it can be easily cleaned with alcohol or the like.

The control unit 30 is provided with a control unit such as an LED driving power supply unit and a PLC (programmable logic controller) inside a housing 37, drives and controls a plurality of LED elements 24 provided in the light source unit 20, and controls the light source unit The irradiance of the light emitted by 20, emission time, etc. can be controlled.

The irradiance immediately below the light irradiation window 28 is preferably 33 mW/cm ² or more and 150 mW/cm ² or less. The term “directly below the light irradiation window 28” refers to a range less than 3 cm from the light irradiation window 28, for example.
Here, the irradiance of 33 mW/cm ² is necessary to obtain an ultraviolet irradiation dose (accumulated irradiation dose) of 60 J/cm ² required for treating scleroderma with a treatment time (irradiation time) of 30 minutes. is the irradiance. In general, skin disease treatment devices are required to complete treatment within 30 minutes. On the other hand, if the irradiance is too high, the patient may feel heat and pain during treatment. An irradiance of 150 mW/cm ² is an irradiance that can suppress the heat felt by the patient during treatment and the pain caused by the heat.

Note that the control unit 30 may be capable of individually driving and controlling the plurality of LED elements 24 . In this case, the controller 30 can selectively turn on some of the plurality of LED elements 24 according to the size and shape of the affected area.

When using the phototherapy device 10, the operator grips the manual lever 19 and places the light source unit 20 at a position where the light irradiation window 28 faces the diseased part of the patient. The phototherapeutic apparatus 10 is used in a state in which the diseased site and the light source unit 20 (light irradiation window 28) are in contact with each other or in close proximity (for example, separated by about 3 cm) from the viewpoint of ensuring a stable irradiance. preferably. By operating the graphic operation panel 39 of the control unit 30 by the operator, the LED element 24 to which power is supplied from the control unit 30 is lit in the light source unit 20, and the patient's diseased part is illuminated. Treatment light is irradiated (surface irradiation).

In the phototherapy device 10 of this embodiment, the therapeutic light emitted from the light source unit 20 is light having a peak wavelength within the specific wavelength range. Therefore, by irradiating the affected area with the therapeutic light, collagenase (MMP1), which is an enzyme that decomposes and fragments collagen that causes skin hardening in scleroderma, can be expressed with a significant difference. . Therefore, an excellent therapeutic effect on scleroderma can be obtained.
Further, in the phototherapy device 10 according to the present embodiment, the therapeutic light emitted from the light source unit 20 is substantially cut light having a wavelength of 350 nm or less. Therefore, even when the therapeutic light is applied to the affected area, immediate blackening can be suppressed. This point will be described below based on experimental examples.

(Experimental example 1)
First, 1×10 ⁵ cells of Normal Human Epidermal Melanocytes-Neonatal (HEMn, human melanocytes/neonatal) were seeded in a container such as a 35 mm dish, and the atmosphere in the incubator was maintained at a temperature of 37° C. using a constant humidity incubator. It was cultured for 24 hours under conditions of a carbon dioxide (CO ₂ ) concentration of 5%. After that, the culture solution was removed, and 1 ml of physiological saline (Phosphate buffered saline: PBS) was added.
Then, using an LED irradiator that irradiates light having a peak wavelength in the wavelength range of 365±5 nm, the irradiation conditions shown in Table 1 below are applied so that the irradiation amount (integrated irradiation amount) is 15 J/cm ² . was irradiated with light.
Light irradiation was performed using the following four groups. (1) non-irradiation (control), (2) without filter, (3) with A filter, and (4) with B filter.

Here, the A filter is a filter as an example, and the B filter is a filter as a comparative example.
The filter characteristics of the A and B filters are shown by curves A and B in FIG. Table 2 shows characteristic values of the A filter and the B filter. The A filter substantially cuts light with a wavelength of about 340 nm from the incident light, while the B filter has a characteristic of transmitting a certain amount (around 35%) of the light with a wavelength of about 340 nm from the incident light.

Light having a peak wavelength in the wavelength range of 365±5 nm emitted from the LED illuminator becomes light in which light having a wavelength of 350 nm or less is substantially cut after passing through the A filter. This point will be described below.
FIG. 7 shows the spectrum of the LED element having the lower limit of the center wavelength variation (center wavelength: 360 nm) after passing through the A filter and the B filter. In FIG. 7, the solid line c is the spectrum of the LED element with a center wavelength of 360 nm, the dashed-dotted line A is the spectral transmittance of the A filter, the double-dotted chain line B is the spectral transmittance of the B filter, and the dashed line ca is after the transmission of the A filter. and the dotted line cb is the spectrum of the LED element after passing through the B filter. Each spectrum is normalized with a peak value of 1.
The intensity ratio of light with a wavelength of 350 nm when the peak intensity of the LED element is 1, the intensity ratio of light with a wavelength of 350 nm when the peak intensity of the LED element after transmission through filter A is 1, and the intensity ratio of light with a wavelength of 350 nm after transmission through filter B Table 3 shows the intensity ratio of light with a wavelength of 350 nm when the peak intensity of the LED element is set to 1.

While the intensity ratio of light with a wavelength of 350 nm to the peak wavelength of the LED element is 10%, the intensity ratio of light with a wavelength of 350 nm to the peak wavelength of light after passing through the A filter is 3%. of light is substantially cut. On the other hand, the intensity ratio of light with a wavelength of 350 nm to the peak wavelength of light after passing through the B filter is 8%, and the light after passing through the B filter contains light with a wavelength of 350 nm.
In this way, the LED irradiator is equipped with an A filter to substantially cut light with a wavelength of 350 nm or less, and the LED irradiator is equipped with a B filter and does not cut light with a wavelength of 350 nm or less. did

Next, the PBS was aspirated off, the culture medium was added, and culture was carried out for 8 days using a constant humidity incubator under the conditions of a temperature of 37° C. and a CO ₂ concentration of 5% in the atmosphere of the incubator.
Cells were then harvested and absorbance at 405 nm was measured using a plate reader (SpectraMax 340/Molecular Devices). That is, an immediate blackening reaction was observed. The results are shown in FIG.

In FIG. 8, each measurement result is shown as a relative value when the measurement result at the time of non-irradiation (control) is set to 1. In FIG. As shown in FIG. 8, it can be seen that light irradiation increases absorbance, that is, promotes darkening of melanin. In addition, it was confirmed that the blackening was most accelerated when there was no filter, and the blackening was suppressed by mounting the filter. It was also confirmed that when the A filter was mounted, blackening was suppressed as compared to when the B filter was mounted.

(Experiment 2)
First, 1×10 ³ cells of Normal Human Epidermal Melanocytes-Neonatal (HEMn, human melanocytes/neonatal) were seeded in a container such as a 35 mm dish, and the atmosphere in the incubator was maintained at a temperature of 37° C. using a constant humidity incubator. It was cultured for 24 hours under conditions of a carbon dioxide (CO ₂ ) concentration of 5%. After that, the culture solution was removed, and 1 ml of physiological saline (Phosphate buffered saline: PBS) was added.
Then, using an LED irradiator that irradiates light having a peak wavelength in the wavelength range of 365±5 nm, the irradiation conditions shown in Table 1 above are applied so that the irradiation amount (integrated irradiation amount) is 15 J/cm ² . was irradiated with light.
Light irradiation was performed using the following four groups. (1) no irradiation (control), (2) no filter, (3) A filter installed, and (4) B filter installed. Here, the filter characteristics of the A filter and the B filter are the same as in Experimental example 1.

Next, the PBS was aspirated off, the culture medium was added, and the cells were cultured for 24 hours using a constant humidity incubator under conditions of a temperature of 37° C. and a CO ₂ concentration of 5% in the atmosphere of the incubator.
After that, the cells were collected and the expression of tyrosinase mRNA was measured by real-time PCR. In other words, the delayed blackening reaction was observed. The results are shown in FIG.

In FIG. 9, each measurement result is shown as a relative value when the measurement result at the time of non-irradiation (control) is set to 1. In FIG. As shown in FIG. 9, it can be seen that the expression of tyrosinase mRNA is promoted by light irradiation. It was also confirmed that the expression of tyrosinase mRNA was suppressed when the A filter was mounted, as compared to the case without the filter.

Based on the above experiments, by installing the A filter having the filter characteristics shown in Table 2, the immediate blackening reaction due to light irradiation when light having a peak wavelength in the wavelength range of 365 ± 5 nm is effectively suppressed. I have confirmed that it is possible. In other words, by using the A filter to substantially block light in the vicinity of a wavelength of 340 nm among the emitted light from the LED irradiator, side effects (immediate blackening) due to light irradiation are suppressed, and a good therapeutic effect is obtained. was confirmed to be obtained.

As described above, the light source unit 20 of the phototherapy device 10 in the present embodiment includes a plurality of LED elements 24 that emit light having a peak wavelength in the wavelength range of 365±5 nm, and the emitted light of the plurality of LED elements 4 and a light irradiation window 28 through which is incident and emits therapeutic light. A filter (filter for phototherapeutic device) 26 is attached to the light source unit 20 of the phototherapeutic device 10 . The filter 26 is arranged between the plurality of LED elements 24 and the light irradiation window 28 and substantially blocks light with a wavelength of 350 nm or less among the light emitted from the plurality of LED elements 24 .
In this way, in the phototherapy device 10 using a plurality of LED elements 24 as a light source, in order to reduce side effects (immediate blackening) in anticipation of variations in the peak wavelength of about ±5 nm, a filter that is a short wavelength cut filter 26 is installed.

As a result, the therapeutic light emitted from the light source unit 20 has a peak wavelength in the specific wavelength range of 365±5 nm, and light with a wavelength of 350 nm or less is substantially blocked. In other words, it is possible to irradiate light as therapeutic light that contains light around 365 nm, which provides excellent therapeutic effects for scleroderma, and substantially does not contain light around 340 nm, which causes immediate darkening. Therefore, it is possible to obtain an excellent skin therapeutic effect for scleroderma while suppressing immediate tanning.
For scleroderma, repeated treatments are envisioned. If immediate darkening is repeated with each treatment and long-term pigmentation occurs, the absorption of light in the epidermis increases and the depth of light penetration into the targeted dermis decreases, resulting in a decrease in treatment efficiency. end up

When there is no pigmentation, as shown in FIG. 10(a), the epidermis 110 absorbs less light. Therefore, the light (therapeutic light) UV applied to the skin 100 can reach the dermis 120 where the target cells are located. On the other hand, when pigmentation occurs as shown in FIG. 10B, light is absorbed by blackened melanin 112, and therapeutic light UV does not reach the dermis 120 sufficiently. Therefore, the therapeutic effect is reduced compared to when there is no pigmentation.
In the present embodiment, since immediate darkening can be suppressed as described above, it is possible to suppress pigmentation, maintain the deep penetration of light into the dermis, and maintain treatment efficiency.
Moreover, even when the peak wavelengths of the plurality of LED elements 24 vary, these LED elements can be used as they are as a light source. In other words, it is not necessary to sort out the LED elements in order to align the peak wavelengths of the plurality of LED elements 24 .

Furthermore, by using a colored glass filter as the filter 26, the light utilization efficiency can be improved as compared with the case of using a dielectric multilayer filter as the filter 26, for example. In addition, since the dielectric multilayer filter depends on the incident angle, in order to improve the light utilization efficiency, the dielectric multilayer filter should be used after improving the angular characteristics of the light from the LED using a collimating lens. must be used, which complicates the apparatus and increases its size. In contrast, by using a colored glass filter as the filter 26, the configuration of the apparatus can be simplified, and the size of the apparatus can be reduced.
In this embodiment, the filter 26 may be a filter that can substantially block light with a wavelength of 350 nm or less, preferably 355 nm, among the light emitted from the plurality of LED elements 24. For example, a dielectric It may be a multilayer filter. However, it is preferable to use a colored glass filter from the viewpoint of the light utilization efficiency.

Further, in the phototherapy device 10, the illuminance of the therapeutic light directly below the light irradiation window 28 can be set to 33 mW/cm ² or more and 150 mW/cm ² or less. Thus, by setting the illuminance of the treatment light directly under the light irradiation window to 33 mW/cm ² or more, the treatment time can be shortened. In addition, by setting the illuminance of the treatment light directly below the light irradiation window to 150 mW/cm ^{2 or} less, it is possible to suppress the heat felt by the patient during treatment and the pain caused by the heat.

(Modification)
In this embodiment, the case where the filter 26 is arranged between the plurality of LED elements 24 and the light irradiation window 28 has been described. However, the filter 26 may also serve as the light irradiation window 28 . That is, as shown in FIG. 11, a filter 26 as a light irradiation window may be arranged at the position of the light irradiation window 28 shown in FIG. In this case, since there is no need to dispose a light irradiation window (quartz window), the cost can be reduced accordingly.
Moreover, in the present embodiment, the phototherapy device 10 may be provided with the light source unit 20 and the control unit 30 . That is, the configuration of the light source unit 20 and the configuration of the control unit 30 are not limited to the configurations shown in FIGS. can be used. For example, the phototherapeutic device 10 may be a handy type therapeutic device in which a handle or the like is gripped with one hand and the light source unit 20 is moved to be placed at a desired position during treatment.

DESCRIPTION OF SYMBOLS 10... Phototherapy apparatus, 20... Light source part, 21... Light source unit, 24... LED element, 26... Filter (filter for phototherapy apparatus), 28... Window member (light irradiation window), 30... Control part

Claims (3)

For use in a phototherapeutic device that irradiates therapeutic light to an affected area using a plurality of LED elements that emit light having a peak wavelength in the range of 365±5 nm and including light with a wavelength of 350 nm or less as a light source. is a filter,
Consists of colored glass filters,
The midpoint wavelength between the first wavelength at which the transmittance is 5% and the second wavelength at which the transmittance is 72% is 350 nm or more and 365 nm or less, and the first wavelength and the second wavelength are has a filter characteristic in which the interval is 30 nm or less,
A filter for a phototherapeutic device, wherein light emitted from the plurality of LED elements is incident and transmitted light is emitted as the therapeutic light. 2. A filter for a phototherapeutic apparatus according to claim 1, wherein said first wavelength is 340 nm or less. 3. The filter for phototherapeutic apparatus according to claim 1, wherein the filter has a filter characteristic such that an average value of transmittance in a wavelength range from the second wavelength to 800 nm is 80% or more.

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