JP6829968B2

JP6829968B2 - Culture equipment and culture method

Info

Publication number: JP6829968B2
Application number: JP2016173913A
Authority: JP
Inventors: 卓也塩原
Original assignee: Taikisha Ltd
Current assignee: Taikisha Ltd
Priority date: 2016-09-06
Filing date: 2016-09-06
Publication date: 2021-02-17
Anticipated expiration: 2036-09-06
Also published as: JP2018038309A

Description

本発明は、再生医療などを目的として生体由来（例えば、人由来）の細胞や組織を培養する培養設備、及び、その培養設備を用いて実施する培養方法に関する。
さらに詳しくは、培養物を培養する内部空間を所定の培養環境に保つ培養器を複数設けるとともに、内部で前記培養物に対して培養操作を施す培養操作器を設け、複数の前記培養器の夫々と前記培養操作器との間において前記培養物の受け渡しを行う培養設備、及び、その培養設備を用いて実施する培養方法に関する。 The present invention relates to a culture facility for culturing cells and tissues derived from a living body (for example, human origin) for the purpose of regenerative medicine, and a culture method carried out using the culture facility.
More specifically, a plurality of incubators for maintaining the internal space for culturing the culture in a predetermined culture environment are provided, and a culture operation device for internally performing a culture operation on the culture is provided, and each of the plurality of the incubators is provided. The present invention relates to a culture facility for delivering the culture to and from the culture controller, and a culture method carried out using the culture facility.

従来、この種の培養設備として、下記の特許文献１により提案された培養設備がある。 Conventionally, as this type of culture equipment, there is a culture equipment proposed by Patent Document 1 below.

この特許文献１の提案設備では（図４参照）、培養器としての複数のインキュベート部３２ａ〜３２ｃと、操作部３３と、保管部３７と、入出庫部３５とを備えている。 The proposed equipment of Patent Document 1 (see FIG. 4) includes a plurality of incubation units 32a to 32c as incubators, an operation unit 33, a storage unit 37, and a warehousing / delivery unit 35.

各インキュベート部３２ａ〜３２ｃでは、その内部において培養物（細胞）を培養する。操作部３３では、その内部に配置した操作ロボット３６や培養操作機器３８を用いて培養物を培養操作する。 In each incubation section 32a to 32c, a culture (cell) is cultured inside the incubation section 32a to 32c. The operation unit 33 cultures the culture using the operation robot 36 and the culture operation device 38 arranged inside the operation unit 33.

また、操作部３３は、操作ロボット３６を配置した機器設置部３１と、培養操作機器３８を配置した培養操作部３３ａとに区分されている。 Further, the operation unit 33 is divided into an equipment installation unit 31 in which the operation robot 36 is arranged and a culture operation unit 33a in which the culture operation equipment 38 is arranged.

保管部３７では、培養に必要な試薬類や培養機器を保管し、入出庫部３５では、新たに培養する培養物の搬入及び処理が完了した培養物の搬出を行う。 The storage unit 37 stores reagents and culturing equipment necessary for culturing, and the warehousing / delivery unit 35 carries in the newly cultivated culture and unloads the processed culture.

複数のインキュベート部３２ａ〜３２ｃの夫々は、保管部３７や入出庫部３５とともに、操作部３３における機器設置部３１に対し隣接させて配置し、各インキュベート部３２ａ〜３２ｃと操作部３３との間、保管部３７と操作部３３との間、入出庫部３５と操作部３３との間には、夫々、密閉扉Ｄを設けてある。 Each of the plurality of incubation units 32a to 32c is arranged adjacent to the equipment installation unit 31 in the operation unit 33 together with the storage unit 37 and the warehousing / delivery unit 35, and is located between the incubation units 32a to 32c and the operation unit 33. A closed door D is provided between the storage unit 37 and the operation unit 33, and between the warehousing / delivery unit 35 and the operation unit 33, respectively.

１つのインキュベート部３２ａ〜３２ｃにおいて培養する培養物（細胞）は同一人物に由来するものに限定し、インキュベート部３２ａ〜３２ｃごとに異なる人物に由来する培養物を培養する。 The cultures (cells) to be cultured in one incubation section 32a to 32c are limited to those derived from the same person, and cultures derived from different persons are cultured in each incubation section 32a to 32c.

培養物の培養操作を行う場合は、操作部３３の機器設置部３１に配置した操作ロボット３６により、いずれか１つのインキュベート部３２ａ〜３２ｃから培養物を取り出し、取り出した培養物に対し操作部３３（特に培養操作部３３ａ）において所定の培養操作を施す。 When culturing the culture, the operation robot 36 arranged in the equipment installation unit 31 of the operation unit 33 takes out the culture from any one of the incubation units 32a to 32c, and the operation unit 33 with respect to the taken out culture. (In particular, the culture operation unit 33a) performs a predetermined culture operation.

そして、培養操作を施した培養物は、再び操作ロボット３６により機器設置部３１を通じて元のインキュベート部３２ａ〜３２ｃに戻し、その後（即ち、一人物に由来する培養物の培養操作を行った後）は、全ての密閉扉Ｄを閉じた状態で、操作部３３に対してのみオートクレーブ滅菌を施す。 Then, the cultured culture is returned to the original incubation units 32a to 32c through the equipment installation unit 31 by the operation robot 36 again, and then (that is, after the culture operation of the culture derived from a single person is performed). Performs autoclave sterilization only on the operation unit 33 with all the closed doors D closed.

また、１つのインキュベート部３２ａ〜３２ｃにおける培養物の培養（即ち、一人物に由来する培養物の培養）が完了した場合は、培養が完了した培養物を取り出した後、培養物を取り出したインキュベート部３２ａ〜３２ｃと操作部３３との間の密閉扉Ｄだけを開いた状態で、培養物を取り出した１つのインキュベート部３２ａ〜３２ｃと操作部３３とに対してのみオートクレーブ滅菌を施す。 When the culture of the culture in one incubation section 32a to 32c (that is, the culture of the culture derived from a single substance) is completed, the culture in which the culture is completed is taken out, and then the culture is taken out. With only the closed door D between the parts 32a to 32c and the operation part 33 open, autoclave sterilization is performed only on one incubation part 32a to 32c from which the culture has been taken out and the operation part 33.

一方、保管部３７の滅菌が必要なときには、保管部３７と操作部３３との間の密閉扉Ｄを開いた状態で、保管部３７と操作部３３とに対してオートクレーブ滅菌を施し、同様に、入出庫部３５の滅菌が必要なときには、入出庫部３５と操作部３３との間の密閉扉Ｄを開いた状態で、入出庫部３５と操作部３３とに対してオートクレーブ滅菌を施す。 On the other hand, when the storage unit 37 needs to be sterilized, the storage unit 37 and the operation unit 33 are autoclaved in the same manner with the sealed door D between the storage unit 37 and the operation unit 33 open. When sterilization of the warehousing / delivery unit 35 is required, autoclave sterilization is performed on the warehousing / delivery unit 35 and the operation unit 33 with the sealed door D between the warehousing / delivery unit 35 and the operation unit 33 open.

つまり、この特許文献１の提案設備では、複数の密閉扉Ｄを選択的に開閉して、操作部３３を含む必要部をオートクレーブ滅菌することで、いわゆる交差汚染（即ち、異なる人物に由来する培養物どうしの間で細菌やウイルスなどの感染が生じたり、特定人物に由来する培養物に他人由来の培養物が混入したりするなどの汚染）を防止し、これにより、異なる人物に由来する培養物（細胞）を同時に培養することを可能にして、培養物の生産効率の向上を図っている。 That is, in the proposed equipment of Patent Document 1, a plurality of closed doors D are selectively opened and closed, and the necessary parts including the operation part 33 are autoclaved, so that so-called cross-contamination (that is, culture derived from a different person) is performed. Prevents infections such as bacteria and viruses between objects, and contamination of cultures derived from a specific person with cultures derived from another person), thereby preventing cultures derived from different people. It is possible to cultivate objects (cells) at the same time to improve the production efficiency of the culture.

なお、特許文献１の提案設備では、操作部３３における機器設置部３１と培養操作部３３ａとの間にも密閉シャッタＳを配置し、この密閉シャッタＳを閉じることで、操作ロボット３６を配置した機器設置部３１のみをオートクレーブ滅菌したり、培養操作機器３８を配置した培養操作部３３ａのみをオートクレーブ滅菌したりすることもできるようにしている。 In the proposed equipment of Patent Document 1, a closed shutter S is also arranged between the device installation unit 31 and the culture operation unit 33a in the operation unit 33, and the operation robot 36 is arranged by closing the closed shutter S. Only the device installation unit 31 can be autoclaved, or only the culture operation unit 33a in which the culture operation device 38 is arranged can be autoclaved.

特開２００８−５４６９０号公報Japanese Unexamined Patent Publication No. 2008-54690

しかし、上記した特許文献１の提案設備（図４）では、異なる人物に由来する培養物を各別のインキュベート部３２ａ〜３２ｃに収容して培養する点では、異なる人物に由来する培養物の同時培養が可能であるものの、交差汚染を防止する上で、異なる人物に由来する培養物（即ち、各別のインキュベート部３２ａ〜３２ｃで培養される培養物）を操作部３３における培養操作部３３ａにおいて同時に培養操作することはできず、この点において培養物の生産効率がやはり低く制限されてしまう問題がある。 However, in the above-mentioned proposed equipment of Patent Document 1 (FIG. 4), cultures derived from different persons are simultaneously accommodated and cultured in different incubation sections 32a to 32c. Although culturing is possible, in order to prevent cross-contamination, cultures derived from different persons (that is, cultures cultivated in different incubation units 32a to 32c) are used in the culture operation unit 33a of the operation unit 33. It is not possible to operate the culture at the same time, and in this respect, there is a problem that the production efficiency of the culture is still low and limited.

また、操作部３３において操作ロボット３６が配置される機器設置部３１は、各インキュベート部３２ａ〜３２ｃと操作部３３における培養操作部３３ａとを結ぶ共通路域に相当するが、特許文献１の提案設備では、一人物に由来する培養物を操作部３３における培養操作部３３ａにおいて培養操作する度に、培養操作部３３ａとともに、共通路域に相当する機器設置部３１も含む広い範囲を滅菌処理しなければならず、この点からも培養物の生産効率が低く制限される。 Further, the equipment installation unit 31 in which the operation robot 36 is arranged in the operation unit 33 corresponds to a common path area connecting the incubation units 32a to 32c and the culture operation unit 33a in the operation unit 33, but the proposal of Patent Document 1 In the equipment, every time a culture derived from a single object is cultured in the culture operation unit 33a of the operation unit 33, a wide range including the equipment installation unit 31 corresponding to the common path area is sterilized together with the culture operation unit 33a. In this respect as well, the production efficiency of the culture is low and limited.

この実情に鑑み、本発明の主たる課題は、合理的な設備形態を採ることで、上記問題を解消して、培養物の生産効率を効果的に向上させる点にある。 In view of this fact, the main problem of the present invention is to solve the above-mentioned problems and effectively improve the production efficiency of the culture by adopting a rational equipment form.

本発明の第１特徴構成は培養設備に係り、その特徴は、
培養物を培養する内部空間を所定の培養環境に保つ培養器を複数設けるとともに、内部で前記培養物に対して培養操作を施す培養操作器を、前記培養器の夫々とは離隔した位置に設け、
複数の前記培養器の夫々と前記培養操作器との間において前記培養物の受け渡しを行う培養設備であって、
前記培養操作器として、前記培養物に対して施す培養操作が互いに異なる複数種の培養操作器を含む複数の培養操作器を設け、
複数の前記培養操作器の夫々、及び、複数の前記培養器の夫々は、内部の除染が個別に可能な構成にし、
前記培養物を内部に収容する移動自在な配送容器を設け、
前記配送容器は、複数の前記培養操作器の夫々、及び、複数の前記培養器の夫々に対して接続及び接続解除が可能で、かつ、接続した前記培養操作器との間での前記培養物の受け渡し、及び、接続した前記培養器との間での前記培養物の受け渡しが可能な構成にし、
１つの前記配送容器に収容する１ロットの前記培養物ごとに設定されている処理手順に従って、前記配送容器の移動を制御することで、各ロットの前記培養物を前記処理手順の実行に必要な前記培養操作器又は前記培養器に対して自動配送する運転制御器を設け、
前記運転制御器は、実行が要求された複数の前記処理手順夫々の実行開始タイミングの相互関係を設備の運転効率が最大化される関係に自動調整した状態で、それら複数の前記処理手順に従って前記配送容器の移動を制御する構成にしてある点にある。 The first characteristic composition of the present invention relates to the culture equipment, and the characteristic is
A plurality of incubators for maintaining the internal space for culturing the culture in a predetermined culture environment are provided, and a culture operating device for internally performing a culture operation on the culture is provided at a position separated from each of the incubators. ,
A culture facility that transfers the culture between each of the plurality of culture devices and the culture operation device.
As the culture operator, a plurality of culture operators including a plurality of types of culture operators in which the culture operations performed on the culture are different from each other are provided.
Each of the plurality of the culture operating devices and each of the plurality of the culture devices are configured so that internal decontamination is possible individually.
A movable delivery container for accommodating the culture is provided.
The delivery container can be connected to and disconnected from each of the plurality of culture actuators and each of the plurality of culture vessels, and the culture can be connected to and from the connected culture controller. passing, and, in the delivery configuration capable of the culture between the incubator connected,
By controlling the movement of the delivery container according to the treatment procedure set for each of the cultures of one lot contained in the delivery container, the culture of each lot is required to carry out the treatment procedure. An operation controller for automatically delivering to the culture operator or the culture device is provided.
The operation controller automatically adjusts the mutual relationship of the execution start timings of the plurality of processing procedures requested to be executed so as to maximize the operation efficiency of the equipment, and according to the plurality of processing procedures. The point is that it is configured to control the movement of the delivery container .

この構成の培養設備では、例えば、図３に示すように２つの培養器２ａ，２ｂと、３つの培養操作器３ａ〜３ｃとを設ける場合、次のような運転形態を採ることができる。 In the culture facility having this configuration, for example, when two culture devices 2a and 2b and three culture operation devices 3a to 3c are provided as shown in FIG. 3, the following operation mode can be adopted.

つまり、培養器２ａで培養している培養物ｘ１を、培養器２ａに接続した配送容器６に受け渡す。培養物ｘ１を収容した配送容器６は、移動の上、培養操作器３ａに接続し、配送容器６に収容していた培養物ｘ１を培養操作器３ａに受け渡す。これにより、培養操作器３ａでは、受け取った培養物ｘ１に対して所定の培養操作を施す。 That is, the culture x1 cultivated in the incubator 2a is delivered to the delivery container 6 connected to the incubator 2a. The delivery container 6 containing the culture x1 is moved and connected to the culture operator 3a, and the culture x1 contained in the delivery container 6 is delivered to the culture operator 3a. As a result, the culture operation device 3a performs a predetermined culture operation on the received culture x1.

続いて、培養操作器３ａでの培養操作が終了した培養物ｘ１を、培養操作器３ａに接続した配送容器６に受け渡す。培養物ｘ１を収容した配送容器６は、移動の上、培養操作器３ｃに接続し、配送容器６に収容していた培養物ｘ１を培養操作器３ｃに受け渡す。これにより、培養操作器３ｃでは、受け取った培養物ｘ１に対して、培養操作器３ａでの培養操作に続き、所定の培養操作を施す。 Subsequently, the culture x1 for which the culture operation in the culture operation device 3a has been completed is delivered to the delivery container 6 connected to the culture operation device 3a. The delivery container 6 containing the culture x1 is moved and connected to the culture operation device 3c, and the culture x1 contained in the delivery container 6 is delivered to the culture operation device 3c. As a result, the culture operation device 3c performs a predetermined culture operation on the received culture x1 following the culture operation in the culture operation device 3a.

また、培養操作した培養物ｘ１を配送容器６に受け渡した後の培養操作器３ａでは、次の培養物の培養操作に備えて内部を除染する。 Further, in the culture operating device 3a after the cultured culture x1 is delivered to the delivery container 6, the inside is decontaminated in preparation for the culture operation of the next culture.

一方、この培養物ｘ１の一連の配送及び培養操作に併行する形態で、培養器２ｂで培養している培養物ｘ２を、培養器２ｂに接続した配送容器６に受け渡す。培養物ｘ２を収容した配送容器６は、移動の上、培養操作器３ｂに接続し、配送容器６に収容していた培養物ｘ２を培養操作器３ｂに受け渡す。これにより、培養操作器３ａでの培養物ｘ１の培養操作に併行する形態で、培養操作器３ｂでは、受け取った培養物ｘ２に対して所定の培養操作を施す。 On the other hand, the culture x2 cultivated in the incubator 2b is delivered to the delivery container 6 connected to the incubator 2b in a form parallel to the series of delivery and culturing operation of the culture x1. The delivery container 6 containing the culture x2 is moved and connected to the culture operator 3b, and the culture x2 contained in the delivery container 6 is delivered to the culture operator 3b. As a result, in the form of paralleling the culture operation of the culture x1 in the culture operation device 3a, the culture operation device 3b performs a predetermined culture operation on the received culture x2.

続いて、培養操作器３ｂでの培養操作が終了した培養物ｘ２を、培養操作器３ｂに接続した配送容器６に受け渡す。培養物ｘ２を収容した配送容器６は、移動の上、培養物ｘ１を培養操作した後の除染済の培養操作器３ａに接続し、配送容器６に収容していた培養物ｘ２を培養操作器３ａに受け渡す。これにより、培養操作器３ｃでの培養物ｘ１の培養操作に併行する形態で、培養操作器３ａでは、受け取った培養物ｘ２に対して所定の培養操作を施す。 Subsequently, the culture x2 for which the culture operation in the culture operation device 3b has been completed is delivered to the delivery container 6 connected to the culture operation device 3b. The delivery container 6 containing the culture x2 is moved, connected to the decontaminated culture operator 3a after the culture x1 is cultured, and the culture x2 contained in the delivery container 6 is cultured. Hand over to vessel 3a. As a result, in the form of paralleling the culture operation of the culture x1 in the culture operation device 3c, the culture operation device 3a performs a predetermined culture operation on the received culture x2.

また、培養操作した培養物ｘ２を配送容器６に受け渡した後の培養操作器３ｂでは、次の培養物の培養操作に備えて内部を除染する。 Further, in the culture operating device 3b after the cultured culture x2 is delivered to the delivery container 6, the inside is decontaminated in preparation for the culture operation of the next culture.

その後、培養操作器３ｃでの培養操作が終了した培養物ｘ１は、培養操作部３ｃに接続した配送容器６に受け渡して元の培養器２ａに戻し、また、培養操作した培養物ｘ１を配送容器６に受け渡した後の培養操作部３ｃでは、次の培養物の培養操作に備えて内部を除染する。 After that, the culture x1 that has completed the culture operation in the culture operation device 3c is handed over to the delivery container 6 connected to the culture operation unit 3c and returned to the original incubator 2a, and the culture operation x1 that has been cultured is delivered to the delivery container. In the culture operation unit 3c after the delivery to No. 6, the inside is decontaminated in preparation for the culture operation of the next culture.

同様に、培養操作器３ａでの培養操作が終了した培養物ｘ２も、培養操作部３ａに接続した配送容器６に受け渡して元の培養器２ｂに戻し、また、培養操作した培養物ｘ２を配送容器に受け渡した後の培養操作部３ａでは、次の培養物の培養操作に備えて内部を除染する。 Similarly, the culture x2 after the culture operation in the culture operation device 3a is also delivered to the delivery container 6 connected to the culture operation unit 3a and returned to the original incubator 2b, and the culture operation x2 is delivered. After the product is delivered to the container, the culture operation unit 3a decontaminates the inside in preparation for the culture operation of the next culture.

即ち、この構成の培養設備であれば、異なる生体（特に異なる人物）に由来する細胞や組織の培養物ｘ１，ｘ２を、各別の培養器２ａ，２ｂで同時に併行して培養するのに加えて、それら異なる生体に由来する培養物ｘ１，ｘ２を、夫々、いずれかの培養操作器３ａ〜３ｃを用いて同時に併行して培養操作することができ、これにより、先述した特許文献１の提案設備（図４）などに比べ、培養物の生産効率を効果的に高めることができる。 That is, in the case of the culture equipment having this configuration, in addition to simultaneously culturing the cultures x1 and x2 of cells and tissues derived from different living bodies (particularly different persons) in different incubators 2a and 2b in parallel. Therefore, the cultures x1 and x2 derived from these different living bodies can be simultaneously cultured in parallel using any of the culture operating devices 3a to 3c, whereby the above-mentioned proposal of Patent Document 1 is made. Compared with the equipment (Fig. 4), the production efficiency of the culture can be effectively increased.

また、この構成の培養設備であれば、複数の培養操作器３ａ〜３ｃの夫々、及び、複数の培養器２ａ，２ｂの夫々を、扱う培養物ごとに個別に除染するから、また、配送容器６も、扱う培養物ごとに除染することで、交差汚染も確実に防止することができる。 Further, in the case of the culture equipment having this configuration, the plurality of culture operation devices 3a to 3c and the plurality of culture devices 2a and 2b are individually decontaminated for each culture to be handled. By decontaminating the container 6 for each culture to be handled, cross-contamination can be reliably prevented.

そして、培養操作の度に、使用した培養操作器３ａ〜３ｃ及び配送容器６を除染するにしても、それら培養操作器３ａ〜３ｃや配送容器６の個々を除染するだけで済むから、特許文献１の提案設備（図４）のように、各インキュベート部３２ａ〜３２ｃ（各培養器に相当）と操作部３３の培養操作部３３ａ（培養操作器に相当）とを結ぶ共通路域としての機器設置部３１の全体を、培養操作部３３ａとともに、培養操作の度に滅菌（除染）するのに比べて、各回の除染を軽微なもので済ませることができ、この点からも、培養物の生産効率を一層効果的に高めることができる。 Even if the culture operating devices 3a to 3c and the delivery container 6 used are decontaminated each time the culture operation is performed, it is only necessary to decontaminate the culture operation devices 3a to 3c and the delivery container 6 individually. As a common passage area connecting the incubation units 32a to 32c (corresponding to each incubator) and the culture operation unit 33a (corresponding to the culture operation device) of the operation unit 33 as in the proposed equipment of Patent Document 1 (FIG. 4). Compared to sterilizing (decontaminating) the entire equipment installation unit 31 of the above together with the culture operation unit 33a at each culture operation, decontamination can be performed each time with a minor amount. The production efficiency of the culture can be increased more effectively.

さらに、この構成の培養設備であれば、複数の培養操作器３ａ〜３ｂのうちに、培養物ｘ１，ｘ２に対して施す培養操作が互いに異なる複数種の培養操作器（言わば、操作内容が互いに異なる専用の培養操作器）を含めるから、使用する培養操作器３ａ〜３ｃの組み合わせにより多様な培養操作を各培養物ｘ１，ｘ２に対して施すようにしながらも、種々の培養操作が可能な高機能の同種の培養操作器を単に複数配備するなどに比べて、設備費も効果的に低減することができる。 Further, in the case of the culture equipment having this configuration, among the plurality of culture operation devices 3a to 3b, a plurality of types of culture operation devices (so to speak, the operation contents are different from each other) in which the culture operations performed on the cultures x1 and x2 are different from each other. Since different dedicated culture operation devices) are included, various culture operations can be performed while performing various culture operations on each culture x1 and x2 by combining the culture operation devices 3a to 3c to be used. The equipment cost can be effectively reduced as compared with simply deploying a plurality of culture operators having the same function.

なお、本書において言う「同時に併行して」とは、一方の開始時点及び終了時点が他方の開始時点及び終了時点と一致する場合に限らず、一方の実施期間と他方の実施期間とに重複部分があることを意味するものであり、その重複部分は一方の実施期間中における一部期間であってもよく、また、他方の実施期間中における一部期間であってもよい。 The term "parallel at the same time" as used in this document is not limited to the case where one start time and end time coincide with the other start time and end time, and the overlap between one implementation period and the other implementation period. It means that there is, and the overlapping portion may be a part of the period in one implementation period, or may be a part of the period in the other implementation period.

また、上記第１特徴構成では、Further, in the above-mentioned first feature configuration,
１つの前記配送容器に収容する１ロットの前記培養物ごとに設定されている処理手順に従って、前記配送容器の移動を制御することで、各ロットの前記培養物を前記処理手順の実行に必要な前記培養操作器又は前記培養器に対して自動配送する運転制御器を設けてあるから、次の作用・効果も併せて得ることができる。By controlling the movement of the delivery container according to the treatment procedure set for each of the cultures of one lot contained in the delivery container, the culture of each lot is required to carry out the treatment procedure. Since the culture operating device or the operation controller that automatically delivers to the culture device is provided, the following actions and effects can also be obtained.

この構成では（図３参照）、各ロットの培養物ｘ１，ｘ２を、それら各ロットの培養物ｘ１，ｘ２ごとに設定されている処理手順ｒ１，ｒ２の実行に必要な培養操作器３ａ〜３ｃ又は培養器２ａ，２ｂに配送して、それら配送先の培養操作器３ａ〜３ｃで培養操作する又は配送先の培養器２ａ，２ｂで培養することで、各ロットの培養物ｘ１，ｘ２に対して、それら各ロットの培養物ｘ１，ｘ２ごとに設定されている処理手順ｒ１，ｒ２の夫々を実行する。In this configuration (see FIG. 3), the cultures x1 and x2 of each lot are subjected to the culture operating devices 3a to 3c necessary for executing the processing procedures r1 and r2 set for each of the cultures x1 and x2 of each lot. Alternatively, by delivering to the incubators 2a and 2b and culturing in the culture operating devices 3a to 3c of the delivery destination or culturing in the incubators 2a and 2b of the delivery destination, the cultures x1 and x2 of each lot can be treated. Then, each of the processing procedures r1 and r2 set for each of the cultures x1 and x2 of each lot is executed.

そして、この処理手順ｒ１，ｒ２の実行において、設定された処理手順ｒ１，ｒ２に従って配送容器６の移動を運転制御器により制御することで、各ロットの培養物ｘ１，ｘ２を必要な培養操作器３ａ〜３ｃ又は必要な培養器２ａ，２ｂに対して自動配送するから、配送容器６を手作業により移動させて各ロットの培養物ｘ１，ｘ２を必要な培養操作器３ａ〜３ｃや培養器２ａ，２ｂに配送するのに比べ、各ロットの培養物ｘ１，ｘ２ごとに設定されている処理手順ｒ１，ｒ２の夫々を一層容易に、また、誤配送を防止して一層確実に実行することができる。Then, in the execution of the processing procedures r1 and r2, the movement of the delivery container 6 is controlled by the operation controller according to the set processing procedures r1 and r2, so that the cultures x1 and x2 of each lot are required for the culture operating device. Since automatic delivery is performed to 3a to 3c or the necessary incubators 2a and 2b, the delivery container 6 is manually moved to move the cultures x1 and x2 of each lot to the necessary incubators 3a to 3c and the incubators 2a. , 2b, the processing procedures r1 and r2 set for each of the cultures x1 and x2 of each lot can be performed more easily and more reliably by preventing erroneous delivery. it can.

さらに、上記第１特徴構成では、Further, in the above first feature configuration,
前記運転制御器は、実行が要求された複数の前記処理手順夫々の実行開始タイミングの相互関係を設備の運転効率が最大化される関係に自動調整した状態で、それら複数の前記処理手順に従って前記配送容器の移動を制御する構成にしてあるから、次の作用・効果も併せて得ることができる。The operation controller automatically adjusts the mutual relationship of the execution start timings of the plurality of processing procedures requested to be executed so as to maximize the operation efficiency of the equipment, and according to the plurality of processing procedures. Since it is configured to control the movement of the delivery container, the following actions and effects can also be obtained.

つまり（図３参照）、２つの処理手順ｒ１，ｒ２を実行するのに、それら２つの処理手順ｒ１，ｒ２を同時に開始して実行するのでは、１つの培養操作器３ａ〜３ｃ又は１つの培養器２ａ，２ｂを２つのロットの培養物ｘ１，ｘ２の培養操作や培養に同時に重複して使用することが必要になるような場合でも、それら２つの処理手順ｒ１，ｒ２の実行開始タイミングに適当な時間差を与えるだけで、１つの培養操作器３ａ〜３ｃ又は１つの培養器２ａ，２ｂの同時の重複使用を回避した状態で、それら２つの処理手順ｒ１，ｒ２を併行して実行することが可能になり、そのことで、設備の運転効率を高めることが可能になることがある。That is (see FIG. 3), if the two processing procedures r1 and r2 are executed and the two processing procedures r1 and r2 are started and executed at the same time, one culture operating device 3a to 3c or one culture is executed. Even when it is necessary to use the vessels 2a and 2b in duplicate for the culture operation and culture of the cultures x1 and x2 of two lots at the same time, it is appropriate for the execution start timing of those two processing procedures r1 and r2. It is possible to execute the two processing procedures r1 and r2 in parallel while avoiding the simultaneous duplicate use of one culture operation device 3a to 3c or one culture device 2a, 2b only by giving a time difference. It will be possible, which may make it possible to improve the operating efficiency of the equipment.

即ち、上記構成では、このような場合に、１つの培養操作器３ａ〜３ｃ又は１つの培養器２ａ，２ｂの同時の重複使用を回避して複数の処理手順ｒ１、ｒ２を極力併行して実行できる状態に、実行が要求された複数の処理手順ｒ１，ｒ２夫々の実行開始タイミングの相互関係を予め自動調整し、これにより、設備の運転効率を最大化する。That is, in the above configuration, in such a case, a plurality of processing procedures r1 and r2 are executed in parallel as much as possible while avoiding simultaneous duplicate use of one culture operation device 3a to 3c or one culture device 2a and 2b. The mutual relationship between the execution start timings of the plurality of processing procedures r1 and r2 requested to be executed is automatically adjusted in advance so as to be possible, thereby maximizing the operating efficiency of the equipment.

したがって、この構成によれば、設備の運転効率（換言すれば、培養物ｘ１，ｘ２の生産効率）を一層確実かつ効果的に高めることができる。Therefore, according to this configuration, the operating efficiency of the equipment (in other words, the production efficiency of the cultures x1 and x2) can be further and effectively increased.

第１特徴構成の実施にあたり、複数の培養操作器３ａ〜３ｃの夫々は、培養物ｘ１，ｘ２に対する培養操作を担当者が手作業により行うもの、あるいは、培養物ｘ１，ｘ２に対する培養操作をコンピュータ制御などにより自動化ないし半自動化したもののいずれであってもよい。In carrying out the first characteristic configuration, in each of the plurality of culture operating devices 3a to 3c, the person in charge manually performs the culture operation on the cultures x1 and x2, or the culture operation on the cultures x1 and x2 is performed by a computer. It may be either automated or semi-automated by control or the like.

また、培養操作器３ａ〜３ｃないし培養器２ａ，２ｂに対する配送容器６の接続・接続解除操作は、コンピュータ制御などにより自動化ないし半自動化したものであってもよい。Further, the operation of connecting / disconnecting the delivery container 6 to the culture operating devices 3a to 3c or the incubators 2a and 2b may be automated or semi-automated by computer control or the like.

本発明の第２特徴構成は、第１特徴構成の実施に好適な実施形態を特定するものであり、その特徴は、The second feature configuration of the present invention specifies an embodiment suitable for carrying out the first feature configuration, and the feature is
前記配送容器を複数設けてある点にある。The point is that a plurality of the delivery containers are provided.

この構成によれば、２つの培養器２ａ，２ｂと、３つの培養操作器３ａ〜３ｃとを設ける前掲の例（図３）の場合、培養器２ａ−培養操作器３ａ−培養操作器３ｃ−培養器２ａの順に移動させる培養物ｘ１の配送と、培養器２ｂ−培養操作器３ｂ−培養操作器３ａ−培養器２ｂの順に移動させる培養物ｘ２の配送とを、各別の配送容器６を用いて同時に併行して行うことができ、これにより、培養物ｘ１，ｘ２の同時培養及び同時培養操作に対する設備の機能性を効果的に高めることができ、この点からも培養物の生産効率を一層効果的に高めることができる。According to this configuration, in the case of the above-mentioned example (FIG. 3) in which two incubators 2a and 2b and three culture operating devices 3a to 3c are provided, the incubator 2a-culture operating device 3a-culture operating device 3c- Delivery of the culture x1 to be moved in the order of the incubator 2a and delivery of the culture x2 to be moved in the order of the incubator 2b-culture operator 3b-culture operator 3a-incubator 2b are provided in separate delivery containers 6. It can be used in parallel at the same time, which can effectively enhance the functionality of the equipment for simultaneous culture and simultaneous culture operation of the cultures x1 and x2, and from this point as well, the production efficiency of the culture can be improved. It can be enhanced more effectively.

本発明の第３特徴構成は、第１又は第２特徴構成の実施に好適な実施形態を特定するものであり、その特徴は、The third feature configuration of the present invention specifies an embodiment suitable for carrying out the first or second feature configuration, and the feature is
前記配送容器の接続及び接続解除が可能で、かつ、接続した前記配送容器の内部を除染する容器用除染器を設けてある点にある。It is possible to connect and disconnect the delivery container, and a container decontamination device for decontaminating the inside of the connected delivery container is provided.

この構成によれば（図３参照）、扱う培養物ｘ１，ｘ２ごとに配送容器６を除染するのに、収容した培養物ｘ１，ｘ２を取り出して空になった配送容器６を、移動の上、容器用除染器４に接続するだけで、その配送容器６を次の使用に備えて除染することができる。According to this configuration (see FIG. 3), in order to decontaminate the delivery container 6 for each of the cultures x1 and x2 to be handled, the delivery container 6 that has been emptied by taking out the contained cultures x1 and x2 is moved. Above, the delivery container 6 can be decontaminated in preparation for the next use simply by connecting to the container decontamination device 4.

因みに、配送容器６を除染するには、使用後の空になった配送容器６を、複数の培養操作器３ａ〜３ｃのうちの空いた培養操作器や、複数の培養器２ａ，２ｂのうちの空いた培養器に接続して、それら空いた培養操作器や空いた培養器の除染機能により配送容器６を除染することも考えられる。Incidentally, in order to decontaminate the delivery container 6, the empty delivery container 6 after use may be subjected to the empty culture operation device among the plurality of culture operation devices 3a to 3c, or the plurality of culture operation devices 2a and 2b. It is also conceivable to connect to our vacant incubator and decontaminate the delivery container 6 by the decontamination function of those vacant culture operators and vacant incubators.

しかし、この場合、配送容器６を除染している間、配送容器６の除染に使用している培養操作器３ａ〜３ｃないし培養器２ａ，２ｂが、本来の培養操作や培養に使用できない状態になることで、培養物ｘ１，ｘ２の同時培養や同時培養操作に制限が生じる可能性がある。However, in this case, while the delivery container 6 is being decontaminated, the culture operation devices 3a to 3c or the incubators 2a and 2b used for decontaminating the delivery container 6 cannot be used for the original culture operation or culture. The state may limit the simultaneous culture and simultaneous culture operation of the cultures x1 and x2.

これに対し、容器用除染器４を設ける上記構成であれば、そのような制限が生じることを回避することができ、この点からも培養物ｘ１，ｘ２の生産効率を効果的に高めることができる。On the other hand, in the above configuration in which the container decontamination device 4 is provided, it is possible to avoid such restrictions, and from this point as well, the production efficiency of the cultures x1 and x2 can be effectively increased. Can be done.

本発明の第４特徴構成は、第１〜第３特徴構成のいずれかの実施に好適な実施形態を特定するものであり、その特徴は、The fourth feature configuration of the present invention specifies an embodiment suitable for carrying out any of the first to third feature configurations, and the feature is
前記配送容器を複数設け、A plurality of the delivery containers are provided,
前記運転制御器は、前記配送容器から前記培養操作器又は前記培養器に受け渡した前記培養物が、その培養操作器又は培養器から再び前記配送容器に受け渡されるまで、前記培養操作器又は前記培養器と前記配送容器との接続を保持する構成にしてある点にある。The operation controller is the culture controller or the culture until the culture delivered from the delivery container to the culture controller or the incubator is delivered again from the culture controller or the incubator to the delivery container. The point is that it is configured to maintain the connection between the incubator and the delivery container.

この構成によれば（図３参照）、例えば、配送容器６から培養操作器３ａ〜３ｃ又は培養器２ａ，２ｂに培養物ｘ１，ｘ２を受け渡すと、その度に、培養操作器３ａ〜３ｃ又は培養器２ａ，２ｂと配送容器６との接続を解除して、その配送容器６を除染の上で別の培養物の配送に使用するのに比べ、配送容器６に対する除染の必要頻度を効果的に低減することができ、また、配送容器６での交差汚染の発生も一層確実に防止することができる。According to this configuration (see FIG. 3), for example, when the cultures x1 and x2 are delivered from the delivery container 6 to the culture operators 3a to 3c or the incubators 2a and 2b, the culture operators 3a to 3c are delivered each time. Alternatively, the frequency of decontamination of the delivery container 6 is required as compared with the case where the incubators 2a and 2b are disconnected from the delivery container 6 and the delivery container 6 is used for delivery of another culture after decontamination. Can be effectively reduced, and the occurrence of cross-contamination in the delivery container 6 can be more reliably prevented.

培養方法に関する参考例を挙げると、
１つの前記配送容器に収容する１ロットの前記培養物ごとに設定されている処理手順に従って、手動により前記配送容器を移動させることで、各ロットの前記培養物を前記処理手順の実行に必要な前記培養操作器又は前記培養器に対して配送することが考えられる。 To give a reference example of the culture method,
By manually moving the delivery container according to the treatment procedure set for each of the cultures of one lot contained in the delivery container, the culture of each lot is required to carry out the treatment procedure. It is conceivable to deliver to the culture operating device or the culture device .

この参考例の培養方法では（図３参照）、先と同様、各ロットの培養物ｘ１，ｘ２を、それら各ロットの培養物ｘ１，ｘ２ごとに設定されている処理手順ｒ１，ｒ２の実行に必要な培養操作器３ａ〜３ｃ又は培養器２ａ，２ｂに配送して、それら配送先の培養操作器３ａ〜３ｃで培養操作する又は配送先の培養器２ａ，２ｂで培養することで、各ロットの培養物ｘ１，ｘ２に対して、それら各ロットの培養物ｘ１、ｘ２ごとに設定されている処理手順ｒ１，ｒ２の夫々を実行する。 In the culture method of this reference example (see FIG. 3), the cultures x1 and x2 of each lot are subjected to the execution of the processing procedures r1 and r2 set for each of the cultures x1 and x2 of each lot as before. Each lot is delivered to the necessary culture operating devices 3a to 3c or incubators 2a and 2b, and the culture is operated in the culture operating devices 3a to 3c at the delivery destination or cultured in the incubators 2a and 2b at the delivery destination. For the cultures x1 and x2 of the above, the processing procedures r1 and r2 set for each of the cultures x1 and x2 of each lot are executed.

そして、この参考例の培養方法では、処理手順ｒ１，ｒ２を実行するのに、設定された処理手順ｒ１，ｒ２に従って配送容器６を手動により移動させるから、配送容器６の移動を自動化する場合に比べ、設備構成を簡略にして設備コストを低減することができる。 Then, in the culture method of this reference example, the delivery container 6 is manually moved according to the set processing procedures r1 and r2 in order to execute the processing procedures r1 and r2. Therefore, when the movement of the delivery container 6 is automated. In comparison, the equipment configuration can be simplified and the equipment cost can be reduced.

上記参考例の培養方法では、
前記培養物を収容した前記配送容器において、収容した前記培養物に対して設定されている前記処理手順を表示することも考えられる。 In the culture method of the above reference example,
It is also conceivable to display the processing procedure set for the contained culture in the delivery container containing the culture .

このようにすれば（図３参照）、培養物ｘ１，ｘ２を収容した配送容器６を作業者が手動により移動させて、配送容器６に収容された培養物ｘ１，ｘ２を必要な培養操作器３ａ〜３ｃ又は培養器２ａ，２ｂに配送するのに、作業者は、配送容器６において表示された収容培養物ｘ１，ｘ２に対する処理手順ｒ１，ｒ２を確認して配送容器６を移動させることができ、これにより、配送容器６を手動により移動させる方式を採りながらも、培養物ｘ１，ｘ２を必要な培養操作器３ａ〜３ｃ又は培養器２ａ，２ｂに対し、誤配送を防止した状態で確実に配送することができる。 In this way (see FIG. 3), the operator manually moves the delivery container 6 containing the cultures x1 and x2, and the cultures x1 and x2 contained in the delivery container 6 are required as a culture operator. In order to deliver to 3a to 3c or the incubators 2a and 2b, the operator may move the delivery container 6 after confirming the processing procedures r1 and r2 for the contained cultures x1 and x2 displayed in the delivery container 6. This allows the cultures x1 and x2 to be reliably delivered to the required culture operators 3a to 3c or incubators 2a and 2b while preventing erroneous delivery, while adopting a method of manually moving the delivery container 6. Can be delivered to.

更に、上記参考例の培養方法では、
実行が要求された複数の前記処理手順夫々の実行開始タイミングの相互関係を設備の運転効率が最大化される関係に調整した状態で、それら複数の前記処理手順に従って前記配送容器を移動させることも考えられる。 Furthermore, in the culture method of the above reference example,
In execution operation efficiency of the interrelationship facilities of a plurality of said processing procedures each of execution start timing is required to adjust the relationship to be maximized state, also move the shipping container in accordance with the plurality of said processing procedures Conceivable.

つまり、前述したように（図３参照）、２つの処理手順ｒ１，ｒ２を実行するのに、それら２つの処理手順ｒ１，ｒ２を同時に開始して実行するのでは、１つの培養操作器３ａ〜３ｃ又は１つの培養器２ａ，２ｂを２つのロットの培養物ｘ１，ｘ２の培養操作や培養に同時に重複して使用することが必要になるような場合でも、それら２つの処理手順ｒ１，ｒ２の実行開始タイミングに適当な時間差を与えるだけで、１つの培養操作器３ａ〜３ｃ又は１つの培養器２ａ，２ｂの同時の重複使用を回避した状態で、それら２つの処理手順ｒ１，ｒ２を併行して実行することが可能になり、そのことで、設備の運転効率を高めることが可能になることがある。 That is, as described above (see FIG. 3), if the two processing procedures r1 and r2 are executed, but the two processing procedures r1 and r2 are started and executed at the same time, one culture operator 3a to Even when it is necessary to simultaneously use 3c or one incubator 2a, 2b for culturing or culturing two lots of cultures x1 and x2, those two treatment procedures r1 and r2 By simply giving an appropriate time difference to the execution start timing, the two processing procedures r1 and r2 are performed in parallel while avoiding the simultaneous duplicate use of one culture operation device 3a to 3c or one culture device 2a, 2b. It may be possible to improve the operating efficiency of the equipment.

即ち、上記方法では、このような場合に、１つの培養操作器３ａ〜３ｃ又は１つの培養器２ａ，２ｂの同時の重複使用を回避して複数の処理手順ｒ１，ｒ２を極力併行して実行できる状態に、実行が要求された複数の処理手順ｒ１，ｒ２夫々の実行開始タイミングの相互関係を予め調整し、これにより、設備の運転効率を最大化する。 That is, in the above method, in such a case, a plurality of processing procedures r1 and r2 are executed in parallel as much as possible while avoiding simultaneous duplicate use of one culture operation device 3a to 3c or one culture device 2a, 2b. The mutual relationship between the execution start timings of the plurality of processing procedures r1 and r2 requested to be executed is adjusted in advance so as to be possible, thereby maximizing the operating efficiency of the equipment.

したがって、配送容器６を手動により移動させる方式を採りながらも、設備の運転効率（換言すれば、培養物ｘ１、ｘ２の生産効率）を一層確実かつ効果的に高めることができる。 Thus, while taking the method of moving manually the distribution feed container 6, (in other words, the production efficiency of the culture x1, x2) operating efficiency of the facilities can be increased more reliably and effectively.

培養設備の全体構成を示す平面図Top view showing the overall configuration of the culture equipment 別実施形態を示す培養設備の平面図Top view of culture equipment showing another embodiment 本発明の作用効果を説明する概略説明図Schematic diagram for explaining the action and effect of the present invention 培養設備の従来例を示す概略平面図Schematic plan view showing a conventional example of a culture facility

図１は、再生医療などを目的として生体由来の細胞や組織を培養する培養設備を示し、１は培養生産室であり、この培養生産室１は、天井部に配置した高性能フィルタを通じて室内に清浄空気を供給するクリーンルームにしてある。 FIG. 1 shows a culture facility for culturing cells and tissues derived from a living body for the purpose of regenerative medicine, etc., and 1 is a culture production room, and this culture production room 1 is indoors through a high-performance filter arranged on the ceiling. It is a clean room that supplies clean air.

培養生産室１の室内には、２基の培養器２ａ，２ｂと、５基の培養操作器３ａ〜３ｅと、１基の容器用除染器４とを設置してあり、５基の培養操作器３ａ〜３ｅは、培養器２ａ，２ｂの夫々とは離隔した位置に設置してある。 In the culture production room 1, two incubators 2a and 2b, five culture operating devices 3a to 3e, and one container decontamination device 4 are installed, and five cultures are cultured. The actuators 3a to 3e are installed at positions separated from the incubators 2a and 2b, respectively.

培養器２ａ，２ｂは、生体由来の細胞や組織である培養物ｘ１〜ｘ３を１ロットずつ内部に収容して培養する装置（いわゆるインキュベート）であり、各培養器２ａ，２ｂは、培養物ｘ１〜ｘ３を培養する内部空間を所定の培養環境に保つ機能を個別に有する。 The incubators 2a and 2b are devices (so-called incubation) in which one lot of cultures x1 to x3, which are cells or tissues derived from a living body, are housed and cultured, and each of the incubators 2a and 2b is a culture x1. It has a function of individually maintaining the internal space for culturing ~ x3 in a predetermined culture environment.

即ち、別ロットとなる異種の培養物ｘ１〜ｘ３（例えば、異なる人物に由来する細胞や組織）を同時に併行して培養する場合、それら異種の培養物ｘ１〜ｘ３は２基の培養器２ａ，２ｂで各別に培養する。 That is, when different lots of different cultures x1 to x3 (for example, cells and tissues derived from different persons) are simultaneously cultured in parallel, the different cultures x1 to x3 are used in two incubators 2a, Incubate separately in 2b.

これら培養器２ａ，２ｂは、過酸化水素ガスなどの除染ガスにより内部を除染する機能を個別に有し、各培養器２ａ，２ｂは、１ロットの培養物ｘ１〜ｘ３に対する培養に使用した後は、その都度、次ロットの培養物に対する培養に備えて内部を除染する。 These incubators 2a and 2b have a function of individually decontaminating the inside with a decontamination gas such as hydrogen peroxide gas, and each incubator 2a and 2b is used for culturing one lot of cultures x1 to x3. After that, the inside is decontaminated in preparation for culturing for the next lot of culture each time.

５基の培養操作器３ａ〜３ｅは、夫々、内部で各ロットの培養物ｘ１〜ｘ３に所定の培養操作を施す装置であり、第１の培養操作器３ａでは、培養生産室１に持ち込んだ全てのロットの培養物ｘ１〜ｘ３に対して施す前処理操作を行う。 The five culture operation devices 3a to 3e are devices that internally perform a predetermined culture operation on the cultures x1 to x3 of each lot, and in the first culture operation device 3a, they are brought into the culture production room 1. A pretreatment operation is performed on the cultures x1 to x3 of all lots.

また、第５の培養操作器３ｅでは、一連の処理が終了した全てのロットの培養物ｘ１〜ｘ３に対して施す後処理操作を行う。 Further, in the fifth culture operating device 3e, a post-treatment operation is performed on the cultures x1 to x3 of all the lots for which the series of treatments have been completed.

第２〜第４の培養操作器３ｂ〜３ｄも、夫々、各ロットの培養物ｘ１〜ｘ３に対して互いに異なる培養操作を施すものであるが、これら第２〜第４の培養操作器３ｂ〜３ｄで施す培養操作は、いずれも、各ロットの培養物ｘ１〜ｘ３の種別に応じて（換言すれば、培養物ｘ１〜ｘ３の各ロット毎に）実施の要否が選択される操作である。 The second to fourth culture operators 3b to 3d also perform different culture operations on the cultures x1 to x3 of each lot, respectively, but these second to fourth culture operators 3b to 3d also perform different culture operations. Each of the culture operations performed in 3d is an operation in which the necessity of implementation is selected according to the type of the culture x1 to x3 of each lot (in other words, for each lot of the culture x1 to x3). ..

５基の培養操作器３ａ〜３ｅは、各培養器２ａ，２ｂと同じく、過酸化水素ガスなどの除染ガスにより内部を除染する機能を個別に有し、各培養操作器３ａ〜３ｅは、１ロットの培養物ｘ１〜ｘ３に対する培養操作に使用した後は、その都度、次ロットの培養物に対する培養操作に備えて内部を除染する。 Like the incubators 2a and 2b, the five culture operators 3a to 3e individually have a function of decontaminating the inside with a decontamination gas such as hydrogen peroxide gas, and each of the culture operators 3a to 3e has a function of decontaminating the inside. After each use for the culture operation for one lot of culture x1 to x3, the inside is decontaminated in preparation for the culture operation for the culture of the next lot.

培養生産室１の室壁には、各ロットの培養物ｘ１〜ｘ３の出入口として、複数基（図１に示す例では３基）のパスボックス５ａ〜５ｃを装備してあり、新たに培養処理する各ロットの培養物ｘ１〜ｘ３は、いずれかのパスボックス５ａ〜５ｃを通じて培養生産室１に搬入し、また、一連の処理を終了した各ロットの培養物ｘ１〜ｘ３は、いずれかのパスボックス５ａ〜５ｃを通じて培養生産室１から搬出する。 The chamber wall of the culture production chamber 1 is equipped with a plurality of pass boxes 5a to 5c (three in the example shown in FIG. 1) as entrances and exits for the cultures x1 to x3 of each lot, and a new culture treatment is performed. The cultures x1 to x3 of each lot to be carried into the culture production chamber 1 through any of the pass boxes 5a to 5c, and the cultures x1 to x3 of each lot after completing a series of treatments are any of the passes. It is carried out from the culture production room 1 through the boxes 5a to 5c.

また、培養生産室１の室内には、自走式の複数基（図１に示す例では３基）の配送容器６ａ〜６ｃを配備してあり、各ロットの培養物ｘ１〜ｘ３は、いずれかの配送容器６ａ〜６ｃの内部に収容した状態で、それら配送容器６ａ〜６ｃの自走により、いずれかの培養器２ａ，２ｂ、いずれかの培養操作器３ａ〜３ｅ、並びに、いずれかのパスボックス５ａ〜５ｃに対して配送する。 Further, in the culture production room 1, a plurality of self-propelled delivery containers 6a to 6c (three in the example shown in FIG. 1) are provided, and the cultures x1 to x3 of each lot can be any of them. In a state of being housed inside the delivery containers 6a to 6c, any of the incubators 2a and 2b, any of the culture operating devices 3a to 3e, and any of the incubators by self-propelled of the delivery containers 6a to 6c. Deliver to pass boxes 5a-5c.

なお、培養生産室１の床には案内線７を施設してあり、各配送容器６ａ〜６ｃは、案内線７による案内下において培養生産室１の室内を個別に自走移動する。 A guide line 7 is installed on the floor of the culture production room 1, and each of the delivery containers 6a to 6c moves independently in the culture production room 1 under the guidance of the guide line 7.

各配送容器６ａ〜６ｃは、各パスボックス５ａ〜５ｃ、各培養器２ａ，２ｂ、並びに、各培養操作器３ａ〜３ｅに対して個別に接続及び接続解除が可能であり、１つの配送容器６ａ〜６ｃを、１つのパスボックス５ａ〜５ｃ又は１つの培養器２ａ，２ｂ又は１つの培養操作器３ａ〜３ｅに対して接続すると、接続した配送容器６ａ〜６ｃの内部が、周囲空間に対する気密性を保った状態で、接続相手の内部空間に連通し、この連通状態において、接続した配送容器６ａ〜６ｃと接続相手との間で培養物ｘ１〜ｘ３の受け渡しが行われる。 Each delivery container 6a to 6c can be individually connected to and disconnected from each pass box 5a to 5c, each incubator 2a and 2b, and each incubator 3a to 3e, and one delivery container 6a. When ~ 6c is connected to one pass box 5a to 5c or one incubator 2a, 2b or one incubator 3a to 3e, the inside of the connected delivery container 6a to 6c is airtight to the surrounding space. In this communication state, the cultures x1 to x3 are delivered between the connected delivery containers 6a to 6c and the connection partner.

また、１つの配送容器６ａ〜６ｃと接続相手（１つのパスボックス５ａ〜５ｃ又は１つの培養器２ａ，２ｂ又は１つの培養操作器３ａ〜３ｅ）との接続が解除されると、上記連通が遮断されて、接続解除された配送容器６ａ〜６ｃの内部、及び、配送容器６ａ〜６ｃとの接続が解除された接続相手の内部がいずれも独立した状態の空間に戻る。 Further, when the connection between one delivery container 6a to 6c and the connection partner (one pass box 5a to 5c or one incubator 2a, 2b or one incubator 3a to 3e) is released, the above communication is established. The inside of the delivery containers 6a to 6c that have been cut off and disconnected, and the inside of the connection partner that has been disconnected from the delivery containers 6a to 6c both return to the space in an independent state.

各配送容器６ａ〜６ｃは、容器用除染器４に対しても同様に接続及び接続解除が可能であり、１つの配送容器６ａ〜６ｃを容器用除染器４に接続すると、接続した配送容器６ａ〜６ｃの内部に対し容器用除染器４から過酸化水素ガスなどの除染ガスが供給され、これにより、接続した配送容器６ａ〜６ｃの内部が除染される。 The delivery containers 6a to 6c can be connected to and disconnected from the container decontamination device 4 in the same manner, and when one delivery container 6a to 6c is connected to the container decontamination device 4, the connected delivery A decontamination gas such as hydrogen peroxide gas is supplied from the container decontamination device 4 to the insides of the containers 6a to 6c, whereby the insides of the connected delivery containers 6a to 6c are decontaminated.

即ち、一連の処理が終了した培養物ｘ１〜ｘ３をいずれかのパスボックス５ａ〜５ｃに受け渡して空になった使用済の配送容器６ａ〜６ｃは、その都度、容器用除染器４に接続することで内部を除染する。 That is, the used delivery containers 6a to 6c that have been emptied by passing the cultures x1 to x3 that have been subjected to a series of treatments to any of the pass boxes 5a to 5c are connected to the container decontamination device 4 each time. Decontaminate the inside by doing.

つまり、この培養設備では、別ロットとなる異種の培養物ｘ１〜ｘ３を複数の培養器２ａ，２ｂで各別に培養して、それら培養器２ａ，２ｂの夫々は１ロットの培養物ｘ１〜ｘ３の培養毎に個別に除染するのに加え、複数の培養操作器３ａ〜３ｅを設けて、これら培養操作器３ａ〜３ｅの夫々も１ロットの培養物ｘ１〜ｘ３の培養操作毎に個別に除染し、更に、各ロットの培養物ｘ１〜ｘ３は各別の配送容器６ａ〜６ｃに収容した状態で配送して、それら配送容器６ａ〜６ｃの夫々も１ロットの培養物ｘ１〜ｘ３の配送毎に個別に除染する。 That is, in this culture facility, different lots of different cultures x1 to x3 are separately cultured in a plurality of incubators 2a and 2b, and each of the incubators 2a and 2b is a lot of cultures x1 to x3. In addition to decontaminating each culture individually, a plurality of culture operation devices 3a to 3e are provided, and each of these culture operation devices 3a to 3e is also individually decontaminated for each culture operation of one lot of cultures x1 to x3. Decontamination is performed, and the cultures x1 to x3 of each lot are delivered in a state of being housed in separate delivery containers 6a to 6c, and each of the delivery containers 6a to 6c is also one lot of cultures x1 to x3. Decontaminate individually for each delivery.

このことから、この培養設備では、別ロットとなる異種の培養物ｘ１〜ｘ３どうしの間での交差汚染を確実に防止しながら、それら異種の培養物ｘ１〜ｘ３を同時に併行して培養するとともに、同時に併行して培養操作することができ、これにより、培養物ｘ１〜ｘ３の生産効率を効果的に高めることができる。 For this reason, in this culturing facility, cross-contamination between different kinds of cultures x1 to x3 in different lots is surely prevented, and these different kinds of cultures x1 to x3 are simultaneously cultured in parallel. , The culture can be operated in parallel at the same time, whereby the production efficiency of the cultures x1 to x3 can be effectively increased.

なお、図１は、第１ロットの培養物ｘ１を収容した配送容器６ａを第１の培養操作器３ａに接続して、第１ロットの培養物ｘ１を第１の培養操作器３ａで培養操作（前処理操作）し、それに続いて、第１ロットの培養物ｘ１を、再び配送容器６ａに収容した上で、配送容器６ａの自走により第２の培養操作部３ｂに配送しようとする状態、
及び、これに並行して、第２ロットの培養物ｘ２を収容した配送容器６ｂを培養器２ａに接続して、第２ロットの培養物ｘ２を培養器２ａで培養し、それに続いて、第２ロットの培養物ｘ２を、再び配送容器６ｂに収容した上で、配送容器６ｂの自走により第５の培養操作器３ｅに配送しようとする状態、
並びに、これに併行して、第３ロットの培養物ｘ３を収容した配送容器６ｃを第４の培養操作器３ｄに接続して、第３ロットの培養物ｘ３を第４の培養操作器３ｄで培養操作し、それに続いて、第３ロットの培養物ｘ３を、再び配送容器６ｃに収容した上で、配送容器６ｃの自走により培養器２ｂに配送しようとする状態を示している。 In FIG. 1, the delivery container 6a containing the culture x1 of the first lot is connected to the first culture operation device 3a, and the culture x1 of the first lot is cultured by the first culture operation device 3a. (Pretreatment operation) Subsequently, the culture x1 of the first lot is housed in the delivery container 6a again, and then the delivery container 6a is self-propelled to deliver the culture to the second culture operation unit 3b. ,
In parallel with this, the delivery container 6b containing the second lot of culture x2 is connected to the incubator 2a, the second lot of culture x2 is cultivated in the incubator 2a, and subsequently, the second lot. A state in which 2 lots of culture x2 are housed in the delivery container 6b again, and then the delivery container 6b is self-propelled to deliver the culture to the fifth culture operator 3e.
In parallel with this, the delivery container 6c containing the culture x3 of the third lot is connected to the fourth culture operator 3d, and the culture x3 of the third lot is connected to the fourth culture operator 3d. The culture operation is performed, and subsequently, the culture x3 of the third lot is housed in the delivery container 6c again, and then the delivery container 6c is self-propelled to deliver to the incubator 2b.

８は設備の運転制御を司る運転制御器であり、各配送容器６ａ〜６ｃの移動操作は運転制御器８により自動的に行われる。 Reference numeral 8 denotes an operation controller that controls the operation of the equipment, and the operation of moving each of the delivery containers 6a to 6c is automatically performed by the operation controller 8.

また、各配送容器６ａ〜６ｃの移動操作と連係して、各培養器２ａ，２ｂでは、接続された配送容器６ａ〜６ｃとの間での培養物ｘ１〜ｘ３の受け渡し、収容した培養物ｘ１〜ｘ３の培養、培養環境の調整、培養後の除染などが、運転制御器８により自動的に実施される。 Further, in cooperation with the movement operation of the delivery containers 6a to 6c, in the incubators 2a and 2b, the cultures x1 to x3 are delivered between the connected delivery containers 6a to 6c, and the contained cultures x1. The operation controller 8 automatically performs culturing of ~ x3, adjustment of the culturing environment, decontamination after culturing, and the like.

同様に、各培養操作器３ａ〜３ｅでは、各配送容器６ａ〜６ｃの移動操作と連係して、接続された配送容器６ａ〜６ｃとの間での培養物ｘ１〜ｘ３の受け渡し、収容した培養物ｘ１〜ｘ３に対する培養操作、培養操作環境の調整、培養操作後の除染などが、運転制御器８により自動的に実施される。 Similarly, in each of the culture operating devices 3a to 3e, the cultures x1 to x3 are delivered and housed between the connected delivery containers 6a to 6c in cooperation with the movement operation of the delivery containers 6a to 6c. The operation controller 8 automatically performs the culture operation for the objects x1 to x3, the adjustment of the culture operation environment, the decontamination after the culture operation, and the like.

パスボックス５ａ〜５ｃを通じて培養生産室１に搬入する各ロットの培養物ｘ１〜ｘ３には、各ロットの培養物ｘ１〜ｘ３毎に、処理手順である処理レシピｒ１〜ｒ３を予め設定してあり、これら処理レシピｒ１〜ｒ３には、各培養器２ａ，２ｂ及び各培養操作器３ａ〜３ｅのうちの使用器、使用器の使用順序、各使用器での滞在時間、各使用器での具体的な処理形態などに関する情報を纏めてある。 In the cultures x1 to x3 of each lot carried into the culture production chamber 1 through the pass boxes 5a to 5c, processing recipes r1 to r3, which are processing procedures, are preset for each culture x1 to x3 of each lot. In these processing recipes r1 to r3, the use device of each incubator 2a, 2b and each culture operation device 3a to 3e, the order of use of the use device, the staying time in each use device, and the specifics in each use device. Information on various processing modes is summarized.

これに対し、運転制御器８は、パスボックス５ａ〜５ｃを通じて各ロットの培養物ｘ１〜ｘ３を搬入する際に、それら各ロットの培養物ｘ１〜ｘ３に設定されている処理レシピｒ１〜ｒ３を読み取る。 On the other hand, when the operation controller 8 carries in the cultures x1 to x3 of each lot through the pass boxes 5a to 5c, the processing recipes r1 to r3 set in the cultures x1 to x3 of each lot are used. read.

そして、読み取った処理レシピｒ１〜ｒ３に従って、各ロットの培養物ｘ１〜ｘ３を収容した搬送容器６ａ〜６ｃの自走移動を制御することで、それら配送容器６ａ〜６ｃに収容した各ロットの培養物ｘ１〜ｘ３を、それら各ロットの培養物ｘ１〜ｘ３に設定されている処理レシピｒ１〜ｒ３の実行に必要な培養器２ａ，２ｂ又は必要な培養操作３ａ〜３ｅに対して、それら処理レシピｒ１〜ｒ３の実行に必要な順序及びタイミングで配送する。 Then, by controlling the self-propelled movement of the transport containers 6a to 6c containing the cultures x1 to x3 of each lot according to the read processing recipes r1 to r3, the culture of each lot contained in the delivery containers 6a to 6c is performed. The products x1 to x3 are treated with respect to the incubators 2a and 2b required for executing the treatment recipes r1 to r3 or the necessary culture operations 3a to 3e set in the cultures x1 to x3 of each lot. Deliver in the order and timing required to execute r1 to r3.

また、これに連係して、各培養器２ａ，２ｂでは、配送容器６ａ〜６ｃから受け渡された各ロットの培養物ｘ１〜ｘ３を、それら各ロットの培養物ｘ１〜ｘ３に設定されている処理レシピｒ１〜ｒ３に従って培養し、同様に、各培養操作器３ａ〜３ｃでは、配送容器６ａ〜６ｃから受け渡された各ロットの培養物ｘ１〜ｘ３を、それら各ロットの培養物ｘ１〜ｘ３に設定されている処理レシピｒ１〜ｒ３に従って培養操作する。 Further, in conjunction with this, in the incubators 2a and 2b, the cultures x1 to x3 of each lot delivered from the delivery containers 6a to 6c are set to the cultures x1 to x3 of each lot. Incubate according to the treatment recipes r1 to r3, and similarly, in each of the culture operators 3a to 3c, the cultures x1 to x3 of each lot delivered from the delivery containers 6a to 6c are transferred to the cultures x1 to x3 of each lot. The culture operation is performed according to the processing recipes r1 to r3 set in.

これにより、この培養設備では、各ロットの培養物ｘ１〜ｘ３を、それら各ロットの培養物ｘ１〜ｘ３に設定されている処理レシピｒ１〜ｒ３の通りに培養及び培養操作して、それら各ロットの培養物ｘ１〜ｘ３から、それぞれ所要の種類の生産培養物を得ることができる。 As a result, in this culturing facility, the cultures x1 to x3 of each lot are cultivated and cultivated according to the processing recipes r1 to r3 set in the cultures x1 to x3 of each lot, and each of these lots. The required types of production cultures can be obtained from the cultures x1 to x3 of the above.

また、運転制御器８は、所定期間において生産するべき生産培養物の種類別生産量が指定されるなどして、生産するべき生産培養物の種類別生産量の比が指定されると、その比で各種類の生産培養物を生産するのに必要な複数の処理レシピｒ１〜ｒ３に関し、それら複数の処理レシピｒ１〜ｒ３夫々の実行開始タイミングの相互関係として、設備の運転効率を最大化することができる最適な相互関係Ｋｔを演算する機能を有する。 Further, when the ratio of the production amount of the production culture to be produced is specified by the operation controller 8 by designating the production amount of the production culture to be produced in a predetermined period by type, the operation controller 8 is used. With respect to the plurality of processing recipes r1 to r3 required to produce each type of production culture in proportion, the operation efficiency of the equipment is maximized as the interrelationship between the execution start timings of the plurality of processing recipes r1 to r3. It has a function of calculating the optimum interrelationship Kt that can be performed.

そしてまた、運転制御器８は、それら実行が要求される複数の処理レシピｒ１〜ｒ３夫々の実行開始タイミングの相互関係を、演算した最適な相互関係Ｋｔに自動調整した状態で、それら複数の処理レシピｒ１〜ｒ３に従って配送容器６ａ〜６ｃの自走移動を制御する機能も有する。 Further, the operation controller 8 automatically adjusts the mutual relationship of the execution start timings of the plurality of processing recipes r1 to r3 required to be executed to the calculated optimum mutual relationship Kt, and performs the plurality of processing. It also has a function of controlling the self-propelled movement of the delivery containers 6a to 6c according to the recipes r1 to r3.

したがって、この培養設備では、運転制御器８により演算された最適な相互関係Ｋｔを実現できる範囲内の順序及び時間間隔をもって、所要の処理レシピｒ１〜ｒ３が設定された培養物ｘ１〜ｘ３をパスボックス５ａ〜５ｃに供給するだけで、設備の運転効率（換言すれば、培養物ｘ１〜ｘ３の生産効率）を自動的に最大化することができる。 Therefore, in this culture facility, the cultures x1 to x3 in which the required processing recipes r1 to r3 are set are passed in an order and time interval within a range in which the optimum interrelationship Kt calculated by the operation controller 8 can be realized. By simply supplying the boxes 5a to 5c, the operating efficiency of the equipment (in other words, the production efficiency of the cultures x1 to x3) can be automatically maximized.

図２は、本発明による培養設備の別実施形態を示し、図２に示す培養設備では、各配送容器６ａ〜６ｃを作業者が手作業により移動させるようにしている。 FIG. 2 shows another embodiment of the culture equipment according to the present invention, and in the culture equipment shown in FIG. 2, the operator manually moves each of the delivery containers 6a to 6c.

各配送容器６ａ〜６ｃには、それら配送容器６ａ〜６ｃに収容した各ロットの培養物ｘ１〜ｘ３に設定された処理レシピｒ１〜ｒ３を表示する表示器９を設けてあり、作業者は、この表示器９に表示された処理レシピｒ１〜ｒ３を確認しながら、その表示処理レシピｒ１〜ｒ３の実行に必要な培養操作器３ａ〜３ｃ又は必要な培養器２ａ，２ｂへ各配送容器６ａ〜６ｃを手作業により順次に移動させる。 Each delivery container 6a to 6c is provided with a display 9 for displaying the processing recipes r1 to r3 set in the cultures x1 to x3 of each lot contained in the delivery containers 6a to 6c. While checking the processing recipes r1 to r3 displayed on the display 9, each delivery container 6a to the culture operating devices 3a to 3c required to execute the display processing recipes r1 to r3 or the necessary incubators 2a and 2b. The 6c is manually moved in sequence.

また、この表示器９には、表示した処理レシピｒ１〜ｒ３の最適な実行開始タイミング（即ち、前述した最適相互関係Ｋｔを満足する各処理レシピｒ１〜ｒ３の実行開始タイミング）も表示するようにしてあり、これにより、作業者は、表示器９に表示された処理レシピｒ１〜ｒ３を、同じく表示器９に表示された最適な実行開始タイミングで開始させるように、各配送容器６ａ〜６ｃを移動させることができる。 Further, the display 9 also displays the optimum execution start timing of the displayed processing recipes r1 to r3 (that is, the execution start timing of each processing recipe r1 to r3 satisfying the above-mentioned optimum interrelationship Kt). As a result, the operator sets each of the delivery containers 6a to 6c so that the processing recipes r1 to r3 displayed on the display 9 are started at the optimum execution start timing also displayed on the display 9. Can be moved.

〔別実施形態〕
次に本発明の別実施形態を列記する。 [Another Embodiment]
Next, another embodiment of the present invention will be listed.

配送容器６ａ〜６ｃは、自走移動に代えて、コンベア装置やロボットなどにより移動させるようにしてもよい。 The delivery containers 6a to 6c may be moved by a conveyor device, a robot, or the like instead of the self-propelled movement.

また、配送容器６ａ〜６ｃは、人力による移動に代えて、作業者による遠隔操作で移動させることも考えられ、その場合、作業者が、配送容器６ａ〜６ｃに収容された培養物ｘ１〜ｘ３の設定処理レシピｒ１〜ｒ３を確認しながら、配送容器６ａ〜６ｃの移動を遠隔操作できるようにしてもよい。 Further, it is conceivable that the delivery containers 6a to 6c are moved by remote control by the operator instead of the movement by human power. In that case, the worker moves the cultures x1 to x3 contained in the delivery containers 6a to 6c. The movement of the delivery containers 6a to 6c may be remotely controlled while checking the setting processing recipes r1 to r3 of.

前述の実施形態では、配送容器６ａ〜６ｃから培養操作器３ａ〜３ｅ又は培養器２ａ，２ｂに受け渡した培養物ｘ１〜ｘ３が、その培養操作器３ａ〜３ｅ又は培養器２ａ，２ｂから再び配送容器６ａ〜６ｃに受け渡されるまで、培養操作器３ａ〜３ｅ又は培養器２ａ，２ｂと配送容器６ａ〜６ｃとの接続を保持する例を示したが、これに代えて、配送容器６ａ〜６ｃから培養操作器３ａ〜３ｅ又は培養器２ａ，２ｂに培養物ｘ１〜ｘ３を受け渡す度に、それら培養操作器３ａ〜３ｅ又は培養器２ａ，２ｂと配送容器６ａ〜６ｃとの接続を解除して、接続解除した配送容器６ａ〜６ｃを他の培養物の配送に使用するようにしてもよい。 In the above-described embodiment, the cultures x1 to x3 delivered from the delivery containers 6a to 6c to the culture operating devices 3a to 3e or the incubators 2a and 2b are delivered again from the culture operating devices 3a to 3e or the incubators 2a and 2b. An example of maintaining the connection between the culture operating devices 3a to 3e or the incubators 2a and 2b and the delivery containers 6a to 6c until they are delivered to the containers 6a to 6c has been shown, but instead of this, the delivery containers 6a to 6c Each time the cultures x1 to x3 are delivered to the culture operating devices 3a to 3e or the incubators 2a and 2b, the connection between the culture operating devices 3a to 3e or the incubators 2a and 2b and the delivery containers 6a to 6c is released. The disconnected delivery containers 6a to 6c may be used for delivery of other cultures.

複数の培養操作器３ａ〜３ｅは夫々、培養物ｘ１〜ｘ３を手作業により培養操作するものであってもよく、また、作業者による遠隔操作により培養物ｘ１〜ｘ３に培養操作を施すものであってもよい。 Each of the plurality of culture operating devices 3a to 3e may manually perform a culture operation on the cultures x1 to x3, or perform a culture operation on the cultures x1 to x3 by a remote operation by an operator. There may be.

また、その場合、培養物ｘ１〜ｘ３を手作業により培養操作する作業者や、遠隔操作により培養物ｘ１〜ｘ３に培養操作を施す作業者が、各培養物ｘ１〜ｘ３に設定されている処理レシピｒ１〜ｒ３を確認できるようにしてもよい。 Further, in that case, a worker who manually cultures the cultures x1 to x3 or a worker who remotely controls the cultures x1 to x3 is set to each culture x1 to x3. The recipes r1 to r3 may be confirmed.

配送容器６ａ〜６ｃや培養操作器３ａ〜３ｅ又は培養器２ａ，２ｂ、あるいは、遠隔操作位置において、作業者が培養物ｘ１〜ｘ３に設定されている処理レシピｒ１〜ｒ３を確認できるようにする場合、表示器による処理レシピｒ１〜ｒ３の表示、処理レシピを記載した紙片やカードの掲示による処理レシピｒ１〜ｒ３の表示、あるいは、音声や信号機による処理レシピｒ１〜ｒ３の表示など、どのような表示形態を採用してもよい。 Allow the operator to check the processing recipes r1 to r3 set in the cultures x1 to x3 at the delivery containers 6a to 6c, the culture operating devices 3a to 3e, the incubators 2a, 2b, or the remote control position. In this case, what kind of processing recipes r1 to r3 are displayed by the display, processing recipes r1 to r3 are displayed by posting a piece of paper or a card on which the processing recipes are described, or processing recipes r1 to r3 are displayed by voice or a signal. A display form may be adopted.

本発明は、再生医療を目的として生体由来の細胞や組織を培養する培養設備に限らず、薬品や食品の製造を目的として生体由来の細胞や組織を培養する培養設備など、どのような目的で生体由来の細胞や組織を培養する培養設備にも利用することができる。 The present invention is not limited to a culture facility for culturing living body-derived cells and tissues for the purpose of regenerative medicine, but for any purpose such as a culture facility for culturing living body-derived cells and tissues for the purpose of producing drugs and foods. It can also be used as a culture facility for culturing cells and tissues derived from living organisms.

ｘ１，ｘ２，ｘ３培養物
２ａ，２ｂ培養器
３ａ〜３ｅ培養操作器
６ａ〜６ｃ配送容器
４容器用除染器
ｒ１〜ｒ３処理レシピ（処理手順）
８運転制御器
Ｋｔ処理手順夫々の実行開始タイミングの最適な相互関係 x1, x2, x3 Culture 2a, 2b Incubator 3a to 3e Culture operator 6a to 6c Delivery container 4 Decontamination device for container r1 to r3 Treatment recipe (treatment procedure)
8 Operation controller Kt Processing procedure Optimal interrelationship between execution start timings

Claims

A plurality of incubators for maintaining the internal space for culturing the culture in a predetermined culture environment are provided, and a culture operation device for internally performing the culture operation on the culture is provided.
A culture facility that transfers the culture between each of the plurality of culture devices and the culture operation device.
As the culture operating device, a plurality of culture operating devices including a plurality of types of culture operating devices in which the culture operations performed on the culture are different from each other are provided at positions separated from each of the incubators.
Each of the plurality of the culture operating devices and each of the plurality of the culture devices are configured so that internal decontamination is possible individually.
A movable delivery container for accommodating the culture is provided.
The delivery container can be connected to and disconnected from each of the plurality of culture actuators and each of the plurality of culture vessels, and the culture can be connected to and from the connected culture controller. passing, and, in the delivery configuration capable of the culture between the incubator connected,
By controlling the movement of the delivery container according to the treatment procedure set for each of the cultures of one lot contained in the delivery container, the culture of each lot is required to carry out the treatment procedure. An operation controller for automatically delivering to the culture operator or the culture device is provided.
The operation controller automatically adjusts the mutual relationship of the execution start timings of the plurality of processing procedures requested to be executed so as to maximize the operation efficiency of the equipment, and according to the plurality of processing procedures. A culture facility that is configured to control the movement of delivery containers .

The culture facility according to claim 1, wherein a plurality of the delivery containers are provided.

The culture facility according to claim 1 or 2, wherein the delivery container can be connected and disconnected, and a container decontamination device for decontaminating the inside of the connected delivery container is provided.

A plurality of the delivery containers are provided,
The operation controller is the culture controller or the culture until the culture delivered from the delivery container to the culture controller or the incubator is delivered again from the culture controller or incubator to the delivery container. The culture facility according to any one of claims 1 to 3, which is configured to maintain the connection between the incubator and the delivery container .