JP6803110B2 - External and internal skin preparations containing an extract of nasturtium cultivated by irradiating light with a specific wavelength range - Google Patents

Description

The present invention relates to a novel external or internal skin preparation having excellent antioxidant effect, matrix metalloproteinase (MMP) inhibitory effect, whitening effect and the like.

The skin is located in the outermost layer of the living body, and is an organ that easily generates active oxygen due to the influence of ultraviolet rays and the like, and is constantly exposed to the oxygen stress. On the other hand, active oxygen scavenging enzyme exists in the skin cell and protects the skin cell from the damage of the active oxygen unless the active oxygen exceeding its capacity is generated. However, it is known that the activity of active oxygen scavenging enzyme in skin cells decreases with aging, and when the damage caused by active oxygen exceeds the defense reaction, the skin is oxidized, the cell function deteriorates and aging. It is thought that it will continue. In addition, it is considered that when an organ other than the skin is exposed to active oxygen exceeding its active oxygen scavenging ability, functional deterioration occurs and aging occurs, and various lifestyle-related diseases such as cancer and myocardial infarction develop. Therefore, active oxygen scavengers and antioxidants have been studied for the purpose of protection from damage caused by active oxygen, and active oxygen scavengers such as SOD and catalase, and active oxygen scavengers and antioxidants such as SOD-like active substances are blended. Foods, cosmetics, non-pharmaceutical products, pharmaceuticals, etc. have been developed (see Patent Documents 1 and 2).

The skin is exposed daily to various physical and chemical stresses such as UV, dry, cold, heat and drugs. As a result, skin dysfunction is caused, and various skin aging phenomena become apparent. Wrinkles are one of the skin aging phenomena. It is known that there are two types of wrinkles: epidermal wrinkles and dermal wrinkles. Epidermal wrinkles are called fine wrinkles, which are temporary wrinkles caused by a decrease in the amount of water in the stratum corneum of the epidermis due to dry skin. As a method for improving fine wrinkles, it is common to use cosmetics having a moisturizing effect. On the other hand, dermal wrinkles are wrinkles formed by ultraviolet rays contained in sunlight and aging. The formation mechanism includes promotion of collagen degradation by an increase in matrix metalloproteinase (MMP) in dermal fibroblasts due to ultraviolet rays and aging.

Epidermal wrinkles caused by dryness and dermal wrinkles have different histological morphology, onset mechanism, and treatment method, and dermal wrinkles caused by ultraviolet rays and aging can be improved by using cosmetics having a moisturizing effect. Can not.

So far, for the purpose of improving dermal wrinkles caused by ultraviolet rays, an agent for preventing / improving skin wrinkles containing hydrolyzed almond as an active ingredient (Patent Document 3), extracts of Jochokei, Tenxi and Kisenu An agent for improving wrinkles caused by irradiation with ultraviolet rays containing the active ingredient (Patent Document 4) has been reported.

Collagen is a major structural protein that makes up about one-third of mammalian tissue and is an essential component of many matrix tissues, including cartilage, bone, tendons, and skin. When one site is cleaved by collagenase (MMP-1), which belongs to matrix metalloproteinase (MMP), collagen molecules that are stable in normal tissues are denatured into single-stranded gelatin by various other proteases. It will be disassembled. As a result, the structural integrity of the matrix structure is lost.

Gelatinase (MMP-2, etc.) belonging to MMP is an enzyme produced by fibroblasts, endothelial cells, cancer cells, etc., and is a structural protein that forms a special component of elastic tissues such as collagen, gelatin, and elastin (arteries, tendons, skin, etc.). ) Etc. and laminin 5 which is a main constituent of the basement membrane are decomposed. It has been clarified that its expression and activity are greatly increased by irradiation with ultraviolet rays, cause a decrease in basement membrane components due to ultraviolet rays, cause structural changes in the basement membrane, and become a major factor such as formation of wrinkles and sagging in the skin. (See Non-Patent Document 1). Therefore, a substance having an inhibitory activity against zelatinase is expected to have an effect of suppressing angiogenesis and cancer metastasis in cancer tissues as well as preventing wrinkles and sagging in the skin, and is useful for prevention and treatment of cancer diseases. It is believed that there is. Furthermore, MMP has been reported to be involved in the degradation of extracellular matrix in various pathological conditions such as ulceration, rheumatoid arthritis, osteoporosis, and periodontitis. Therefore, if it has an inhibitory activity on MMP, it is useful for treating and ameliorating various diseases caused by an increase in MMP, such as cancer metastasis, ulceration, rheumatoid arthritis, osteoporosis, and periodontitis.

In general, skin pigmentation seen in age spots, freckles, sunburn, etc. is caused by excessive production of melanin pigment by melanocytes existing in the skin due to abnormal hormones or stimulation of ultraviolet rays, which is deposited in the skin. It is believed that. As one of the methods for preventing such pigmentation, a method for suppressing excessive production of melanin is known. Conventionally, ascorbic acid (vitamin C) and the like have been used for the treatment of pigmentation for internal and external use.

Known documents for nasturtium include scavenging active oxygen, promoting / decomposing hyaluronic acid synthesis, and promoting aquaporin synthesis (Patent Document 5).

On the other hand, as a method for enhancing the medicinal effect of a plant by a plant cultivation method, a method for characteristically increasing functional substances such as vitamins, polyphenols and rutin in the plant has already been reported in patent documents. Patent Document 6 discloses a method for increasing the amount of vitamin A and vitamin E contained in soybean sprouts by irradiating the soybean sprouts with light having a wavelength in the near-ultraviolet to blue region. A cultivation method for increasing α-tocopherol and vitamin C, which are functional substances, by performing artificial ultraviolet irradiation for 5 minutes a day is disclosed, and Patent Document 8 discloses blue light, red light, and far-red light of artificial light sources. A method of increasing vitamin C and vitamin A of Japanese mustard spinach and lettuce by adjusting the intensity of the Japanese mustard spinach is disclosed.

Japanese Unexamined Patent Publication No. 9-118630 Japanese Unexamined Patent Publication No. 9-208484 Japanese Unexamined Patent Publication No. 2000-119125 Japanese Unexamined Patent Publication No. 2006-199611 Japanese Unexamined Patent Publication No. 2004-168732 Japanese Unexamined Patent Publication No. 11-103680 Japanese Unexamined Patent Publication No. 2004-305040 Japanese Unexamined Patent Publication No. 8-205677

Gary J. Fisher et al. , Nature, Vol. 379, No. 25, pp335, 1996

An object of the present invention is to provide a novel external or internal skin preparation having excellent antioxidant effect, matrix metalloproteinase (MMP) inhibitory effect, whitening effect and the like.

As a result of intensive research to solve this problem, the present inventors have applied an antioxidant effect and matrix metalloproteinase to an extract of nastatium cultivated by simultaneously irradiating two kinds of light having a specific wavelength range. We have discovered that the protease (MMP) inhibitory effect and the whitening effect are excellent, and have completed the present invention.

That is, the present invention comprises the following (1) to (7).

(1) Water, lower alcohols and liquid polyhydric alcohols of nastathium cultivated by irradiating light having a photosynthetic photon flux density (PPFD) ratio of 8: 1 to 1: 1 in the wavelength range of 570 to 730 nm and 400 to 515 nm. An external preparation for skin, which comprises an extract from one or more solvents selected from the above.
(2) The external preparation for skin according to claim 1, wherein the photosynthetic photon flux density (PPFD) ratio between the wavelength range of 570 to 730 nm and 400 to 515 nm is 4: 1 to 2: 1.
(3) Nastatium cultivated with a fluorescent lamp or sunlight by cultivating it by irradiating it with light having a photosynthetic photon flux density (PPFD) ratio of 8: 1 to 1: 1 in the wavelength range of 570 to 730 nm and 400 to 515 nm. Nastatium, which is characterized by enhancing one or more effects selected from an antioxidant effect, a matrix metalloproteinase (MMP) inhibitory effect, and a whitening effect.
(4) Nastatium cultivated with a fluorescent lamp or sunlight by cultivating it by irradiating it with light having a photosynthetic photon flux density (PPFD) ratio of 8: 1 to 1: 1 in the wavelength range of 570 to 730 nm and 400 to 515 nm. One or two or more enhanced effects selected from antioxidative effect, matrix metalloproteinase (MMP) inhibitory effect and whitening effect, or one selected from water, lower alcohol and liquid polyhydric alcohol. A drug characterized by containing an extract extracted by two or more kinds of solvents.
(5) Nastatium cultivated with a fluorescent lamp or sunlight by cultivating it by irradiating it with light having a photosynthetic photon flux density (PPFD) ratio of 8: 1 to 1: 1 in the wavelength range of 570 to 730 nm and 400 to 515 nm. One or two or more enhanced effects selected from antioxidative effect, matrix metalloproteinase (MMP) inhibitory effect and whitening effect, or one selected from water, lower alcohol and liquid polyhydric alcohol. A food characterized by containing an extract extracted by two or more kinds of solvents.
(6) An extract of nastathium cultivated by irradiating light in the wavelength range of 570 to 730 nm and / or 400 to 515 nm, which is extracted by one or more solvents selected from water, lower alcohols and liquid polyhydric alcohols. A skin external preparation characterized by containing.
(7) Extracted with one or more solvents selected from nastatium cultivated by irradiating light in the wavelength range of 570 to 730 nm and / or 400 to 515 nm, or water, lower alcohol and liquid polyhydric alcohol of the nastatium. Extract to be.

The nastatium or its extract of the present invention has an excellent antioxidant effect, a matrix metalloproteinase (MMP) inhibitory effect and a whitening effect, and can contribute to the fields of pharmaceuticals, quasi-drugs, cosmetics and foods. is there.

The present invention will be described in detail below.

The extract of Nasturtium used in the present invention is extracted from a part or whole plant such as flowers, fruits, seeds, stems, leaves, roots, etc. It was done. The extraction method is not particularly limited, and for example, it may be extracted by heating or may be extracted at room temperature. Further, in the present invention, instead of the extract, the plant body can be used as it is, or it can be used raw or dried, and can be used properly depending on the purpose. Furthermore, the extract and the plant can be used in combination.

As a cultivation method, it can be cultivated using soil or hydroponics. In the case of hydroponics, the seeds can be sown and then used for hydroponics in a rooted state. Cultivation is preferably carried out in a facility where the temperature, light and carbon dioxide concentration are controlled. The cultivation temperature is 15 to 30 ° C, preferably 20 to 25 ° C. The cultivation period varies depending on the irradiation conditions, but it can be harvested in about 10 to 30 days. It is also possible to grow more.

As the light source, a light source used in a plant cultivation facility or the like can be used, and an optical semiconductor element such as a light emitting diode (LED) or a laser diode can be used, but a light source capable of selectively irradiating a specific wavelength range. If there is, it is not limited to LED.

In the cultivation of nasturtium, the wavelength to be irradiated is preferably blue light having a wavelength range of 400 to 515 nm and red light having a wavelength range of 570 to 730 nm, and more preferably light having a wavelength range of 430 to 460 nm and 630 to 680 nm. It is most preferable to irradiate these lights at the same time. The wavelength at this time refers to the maximum wavelength (peak wavelength) of the irradiation spectrum. As long as it is a light source having a peak of such a wavelength, an original one or a commercially available one can be used. Further, an optical filter may be used so that the above wavelength can be selectively irradiated. In addition to the above two types of light, a light source such as sunlight or a fluorescent lamp can also be used.

The amount of light to be irradiated is expressed as photosynthetic effective photon flux density (PPFD). When irradiating two types of illuminants in combination, it means the total amount of light. The amount of light is preferably 10 to 300 μmol · m ^-2 s ^-1 after germination, and more preferably 50 to 200 μmol · m ^-2 s ^-1 . If the light intensity is outside this range, growth may be impaired or poor growth may occur. Irradiation is preferably performed from a position 10 to 50 cm above the nasturtium. The irradiation time can be appropriately changed according to the characteristics and purpose of the plant, but is preferably 6 hours or more, and more preferably 12 to 24 hours.

The red and blue light intensity ratio means the ratio of each PPFD, and can be selected according to the purpose such as yield and effectiveness.

Among them, in order to increase the yield of plants, the light intensity ratio of red and blue is preferably 1: 0 to 2: 1, and among them, the light intensity ratio of red and blue is particularly high at 4: 1 to 2: 1. Obtained.

In the active oxygen scavenging action (free radical capture / removal action), the light intensity ratio of red and blue is preferably 1: 0 to 0: 1 in terms of effect, and more preferably 4: 1 to 0: 1. Among them, the ratio of red to blue light is most preferably 2: 1 to 0: 1.

In the MMP-1 mRNA expression inhibitory action, the light intensity ratio of red and blue is preferably 1: 0 to 2: 1 in terms of effect. Among them, the ratio of red to blue light is most preferably 8: 1-3: 1.

In the MMP-2 mRNA expression inhibitory action, a light intensity ratio of red to blue of 3: 1 is preferable in terms of effect.

In the melanin production inhibitory action, a red to blue light intensity ratio of 8: 1 to 1: 1 is preferable in terms of effect. Among them, the ratio of red to blue light is most preferably 4: 1 to 2: 1.

Overall, the ratio of red to blue light is preferably 8: 1 to 1: 1 and most preferably 4: 1 to 2: 1.

Examples of the extraction solvent include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol), liquid polyhydric alcohols (1,3-butylene glycol, propylene glycol, glycerin, etc.). ), Ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate, butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.) Can be mentioned. Polar solvents such as water, lower alcohols and liquid polyhydric alcohols are preferred, and water, ethanol, 1,3-butylene glycol and propylene glycol are particularly preferred. These solvents may be used alone or in admixture of two or more.

The above-mentioned extract may be used as it is in the extracted solution, or may be used after concentration, dilution and filtration treatment, decolorization with activated carbon or the like, deodorization treatment and the like, if necessary. Further, the extracted solution may be subjected to treatments such as concentrated drying, spray drying, freeze drying and the like, and used as a dried product. You can also eat it raw, such as salad.

The external preparation or internal preparation of the present invention shall also include foods, and the above-mentioned plants and / or extracts may be used as they are, and cosmetics and pharmaceuticals may be used as long as these effects are not impaired. Oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, moisturizers, which are ingredients used in external products, pharmaceuticals, foods, etc. Ingredients such as agents, powders, ultraviolet absorbers, thickeners, pigments, antioxidants, whitening agents, chelating agents, excipients, film agents, sweeteners and acidulants can also be blended.

The present invention can be used in pharmaceuticals, non-pharmaceutical products, cosmetics, and foods, and its dosage forms include, for example, lotions, creams, massage creams, emulsions, gels, aerosols, packs, cleaning agents, and baths. Agents, foundations, dusting, lipsticks, ointments, poultices, pastes, plasters, essences, powders, pills, tablets, injections, suppositories, emulsions, capsules, granules, liquids (tinki agents, flow extracts) , Suppositories, suspending agents, limonade agents, etc.), tablets, beverages, tea bags, spices, etc.

In the case of external use, the blending amount of the extract used in the present invention is preferably 0.0001% by weight or more, more preferably 0.001 to 10% by weight, in terms of solid matter, based on the total amount. Further, 0.01 to 5% by weight is most preferable. If it is less than 0.0001% by weight, it is difficult to expect a sufficient effect. When it is blended in excess of 10% by weight, it is difficult to recognize the enhancement of the effect and it is uneconomical. On the other hand, in the case of internal use, the dose varies depending on age, body weight, symptoms, therapeutic effect, administration method, treatment time, etc., but usually, the daily dose per adult is preferably 5 mg or more, preferably 10 mg to 5 g. Is more preferable. Further, 100 mg to 1 g is most preferable.

Next, in order to explain the present invention in detail, examples of production, experimental examples, and formulation examples of the extract of nastathium used in the present invention will be given, but the present invention is not limited thereto. The% shown in the production example indicates a weight%, and the compounding amount portion shown in the example indicates a weight portion.

(1) Experimental materials and growth conditions Nastatium seeds are sown on a mesh containing water, germinated in a dark place at a temperature of 22 to 24 ° C, wrapped in a sponge, and placed under a white fluorescent lamp at 22 to 24 ° C for 24 hours. Cultivated and raised seedlings. Then, using a hydroponic cultivation device, the blue LED (peak wavelength 450 nm) and the red LED (peak wavelength 660 nm) are simultaneously irradiated from a position 30 cm above the plant for 24 hours at room temperature 21 to 23 ° C. Cultivation was carried out with the light intensity ratio of red and blue set to 1: 0 to 0: 1 so that the total photosynthetic effective photon flux density of the blue LED was 100 μmol · m ^-2 s ^-1 . Further, as Comparative Example 1, cultivation was carried out under a white fluorescent lamp so that the photosynthetic effective photon bundle density was 100 μmol · m- ² s- ^1, and as Comparative Example 2, cultivation was carried out under sunlight. The light intensity ratio was not changed during cultivation. After cultivating for 4 weeks, it was harvested to obtain raw nasturtium. This was dried with warm air at about 60 ° C. to obtain a dried product of nasturtium (Table 1).

(2) Extraction
Production Example 1A Hot water extract 200 mL of purified water is added to 10 g of the dried product, extracted at 95 to 100 ° C. for 2 hours, filtered, the filtrate is concentrated, and freeze-dried to obtain the hot water extract. Obtained (Table 2).

Production Example 1B 50% ethanol extract 200 mL of 50% ethanol was added to 10 g of the dried product, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain a 50% ethanol extract. (Table 2).

Production Example 1C Ethanol extract 200 mL of ethanol was added to 10 g of the dried product, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain an ethanol extract (Table 2).

Similar to the above, Nastatium cultivated by changing the light intensity ratio of red and blue so that the total photosynthetic effective photon bundle density of red and blue LEDs is 100 μmol · m ^-2 s ^-1 , or photosynthetic effective photon as Comparative Example 1. Nastatium cultivated under white fluorescent lamps under the same growth conditions as above so that the bundle density is 100 μmol · m ^-2 s ^-1, and Nastatium cultivated in sunlight as Comparative Example 2 were used, and Production Examples 1A to 1C described above were used. The same extraction was performed as in Production Examples 2A to 7C, Comparative Production Examples 1A to 1C, and Comparative Production Examples 2A to 2C (Table 2).

Experimental Example 1 Active oxygen scavenging action Free radical capture and removal action was evaluated. Ascorbic acid was used as a positive control. As a model of free radicals, stable free radicals α, α-diphenyl-β-picrylhydrazil (hereinafter referred to as DPPH) are used, and the amount of radicals decreases after reacting with a sample at a constant ratio for a certain period of time. Was measured from the amount of decrease in absorbance at a wavelength of 517 nm.

Method for measuring free radical capture and removal effect Each sample has a final concentration of 0.1 to 1.0 mg / mL for hot water extract and 50% EtOH extract, and a final concentration of 0.03 to 0.05 mg for 100% EtOH extract. 2 mL of absolute ethanol and 1 mL of 0.5 mM DPPH absolute ethanol solution were added to 2 mL of 0.1 M acetate buffer (pH 5.5) added so as to be / mL (0.01 mg / mL for ascorbic acid) to prepare a reaction solution. .. Then, the reaction was carried out at 37 ° C. for 30 minutes, and the absorbance at a wavelength of 517 nm was measured using water as a control. (The 100% EtOH extract was reacted for 70 minutes.) In addition, the absorbance was measured using a reaction solution containing purified water instead of the sample as a blank. The concentration of the sample (IC ₅₀ ) when the absorbance was reduced by 50% as compared to the blank was calculated.

The results of these tests are shown in Table 3. It was found that the extract of the present invention has a stable and excellent free radical trapping and removing action. In particular, the light intensity ratio of red and blue is preferably 1: 0 to 0: 1, more preferably 4: 1 to 0: 1, and most preferably 2: 1 to 0: 1. At these times, a significantly higher effect was observed as compared with the conventional cultivated product using sunlight. Ascorbic acid was inactivated by heat treatment at 100 ° C. for 1 hour, but the activity of the extract of the present invention did not change.

Experimental Example 2 Matrix metalloproteinase (MMP) inhibitory effect The expression levels of MMP-1 and MMP-2 mRNA were measured. Human skin fibroblasts (NB1RGB) were seeded in 1 × 10 ⁵ pieces on a 60 mm dish and cultured in DMEM culture medium containing 10% FBS under 37 ° C. and 5% CO ₂ conditions. When the condition became confluent, each sample was added with a final concentration of 1 μg / mL for MMP-1 mRNA expression measurement, and each sample was added to a DMEM culture solution with a final concentration of 10 μg / mL for MMP-2 mRNA expression measurement. After culturing for 24 hours, total RNA was extracted. Extraction of total RNA from cells was performed using a TRIZOL Reagent (Invitrogen), and the total amount of RNA was determined by absorbance at 260 nm using a spectrophotometer (NanoDrop). The mRNA expression level was measured by the real-time RT-PCR method based on the total RNA extracted from the cells. As the real-time RT-PCR method, SuperScriptIII Platinum Two-Step qRT-PCR Kit with SYBR Green (Invitrogen) was used. That is, after a reverse transcription reaction of 500 ng of total RNA, a PCR reaction (95 ° C: 15 seconds, 60 ° C: 30 seconds, 40 cycles) was performed. For other operations, the expression levels of MMP-1 and MMP-2 mRNA were determined as a ratio to the expression level of β-actin mRNA, which is an internal standard, according to a predetermined method. The MMP expression level was calculated as the ratio of the expression level of MMP mRNA in the sample-added group to the expression level of control MMP mRNA. The primers used to measure the expression level of each gene are as follows.

Primer set for MMP-1 GGGAGATCATCGGGACAACTC (SEQ ID NO: 1)
TGAGCATCCCCCTCCAAATACC (SEQ ID NO: 2)
Primer set for MMP-2 CCGTCGCCCATCATCAA (SEQ ID NO: 3)
CTTTGCATCTTCTTTAGTGTGTCCTT (SEQ ID NO: 4)
Primer set for β-Actin CACTCTTCCAGCCTTCCTCC (SEQ ID NO: 5)
GTGTTGGGCGTACAGGTCTTG (SEQ ID NO: 6)

The results of these experiments are shown in Tables 4 and 5. As a result, the extract of the present invention was found to have an excellent MMP expression inhibitory effect (MMP inhibitory effect). As for the MMP-1 expression suppressing effect, a particularly high effect was observed in which the light intensity ratio of red and blue was 1: 0 to 2: 1. Among them, a particularly high effect was observed in which the light intensity ratio of red and blue was 8: 1-3: 1. Further, in MMP-2, a high effect was observed in which the light intensity ratio of red and blue was 3: 1.

Experimental Example 3 Melanin production suppression test Eagles'MEM (containing 10% fetal bovine serum) containing 3 × 10 ⁴ B16 mouse melanoma cells in the logarithmic growth phase on a 60 mm dish and containing each sample (final concentration 100 μg / mL). The cells were cultured in medium at 37 ° C. under 5% CO ₂ conditions for 5 days. Next, the cells were exfoliated from the dish, ultrasonically crushed, 4N NaOH was added, and the cells were treated at 60 ° C. for 2 hours. D. 475 nm was measured. The cell crushed solution after the ultrasonic treatment was quantified by the Lowry method (J. Biol. Chem., 193,265-275,1951), the amount of melanin per protein amount was calculated, and the amount of melanin without sample was added. Using the amount of production as a control, the melanin production suppression rate was calculated from the value of the amount of melanin produced when the sample was added to the control.

The results of these tests are shown in Table 6. It was found that the extract of the present invention has an excellent melanin production inhibitory effect. In particular, a high effect was observed with a light intensity ratio of red and blue of 8: 1 to 1: 1. Among them, a particularly high effect was observed in which the light intensity ratio of red and blue was 4: 1 to 2: 1.

Prescription example 1 Toner prescription compounding amount (part)
1. 1. Extract of Production Example 3A 1.0
2.1,3-butylene glycol 8.0
3. 3. Glycerin 2.0
4. Xanthan gum 0.02
5. Citric acid 0.01
6. Sodium citrate 0.1
7. Ethanol 5.0
8. Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40EO) 0.1
10. Appropriate amount of fragrance 11. [Manufacturing method] Components 1 to 6 and 11 having a total amount of 100 in purified water and components 7 to 10 are uniformly dissolved, and both are mixed and filtered to obtain a product.

Prescription example 2 Cream prescription compounding amount (part)
1. 1. Extract of Production Example 4A 0.5
2. 2. Squalene 5.5
3. 3. Olive oil 3.0
4. Stearic acid 2.0
5. Beeswax 2.0
6. Octyldodecyl myristate 3.5
7. Polyoxyethylene cetyl ether (20EO) 3.0
8. Behenyl alcohol 1.5
9. Glycerin monostearate 2.5
10. Fragrance 0.1
11. Methyl paraoxybenzoate 0.25
12.1,3-butylene glycol 8.5
13. [Manufacturing method] Ingredients 2 to 9 having a total amount of 100 in purified water are dissolved by heating and mixed, and kept at 70 ° C. to prepare an oil phase. Ingredients 1 and 11 to 13 are heated and dissolved and mixed to prepare an aqueous phase at 75 ° C. An aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring, component 10 is added at 45 ° C., and the mixture is further cooled to 30 ° C. to obtain a product.

In Formulation Example 2, the extracts of Production Example 4A were replaced with the extracts of Production Example 1A, the extracts of Production Example 7A, and the extracts of Production Example 4B, which were designated as Formulation Examples 3, 4 and 5.

Prescription example 6 Emulsion prescription compounding amount (part)
1. 1. Extract of Production Example 2A 1.0
2. 2. Squalene 5.0
3. 3. Olive oil 5.0
4. Jojoba oil 5.0
5. Cetanol 1.5
6. Glycerin monostearate 2.0
7. Polyoxyethylene cetyl ether (20EO) 3.0
8. Polyoxyethylene sorbitan monooleate (20EO) 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12. Methyl paraoxybenzoate 0.2
13. [Manufacturing method] Ingredients 2 to 8 having a total amount of 100 in purified water are dissolved by heating and mixed, and kept at 70 ° C. to prepare an oil phase. Ingredients 1 and 10 to 13 are heated, dissolved and mixed, and kept at 75 ° C. to prepare an aqueous phase. An aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring, component 9 is added at 45 ° C, and the product is further cooled to 30 ° C to obtain a product.

Prescription example 7 Gel agent Prescription compounding amount (part)
1. 1. Extract of Production Example 4C 0.001
2. 2. Ethanol 5.0
3. 3. Methyl paraoxybenzoate 0.1
4. Polyoxyethylene hydrogenated castor oil (60EO) 0.1
5. Appropriate amount of fragrance 6.1,3-butylene glycol 5.0
7. Glycerin 5.0
8. Xanthan gum 0.1
9. Carboxyvinyl polymer 0.2
10. Potassium hydroxide 0.2
11. [Manufacturing method] Ingredients 2 to 5 and components 1 and 6 to 11 having a total amount of 100 in purified water are uniformly dissolved, and the two are mixed to obtain a product.

Prescription example 8 pack prescription compounding amount (part)
1. 1. Extract of Production Example 5A 0.1
2. 2. Extract of Production Example 5B 0.1
3. 3. Polyvinyl alcohol 12.0
4. Ethanol 5.0
5.1,3-butylene glycol 8.0
6. Methyl paraoxybenzoate 0.2
7. Polyoxyethylene hydrogenated castor oil (20EO) 0.5
8. Citric acid 0.1
9. Sodium citrate 0.3
10. Appropriate amount of fragrance 11. [Manufacturing method] Ingredients 1 to 11 having a total amount of 100 are uniformly dissolved in purified water to prepare a product.

Prescription example 9 Foundation prescription compounding amount (part)
1. 1. Extract of Production Example 3A 1.0
2. 2. Stearic acid 2.4
3. 3. Polyoxyethylene sorbitan monostearate (20EO) 1.0
4. Polyoxyethylene cetyl ether (20EO) 2.0
5. Cetanol 1.0
6. Liquid lanolin 2.0
7. Liquid paraffin 3.0
8. Isopropyl myristate 6.5
9. Sodium Carboxymethyl Cellulose 0.1
10. Bentonite 0.5
11. Propylene glycol 4.0
12. Triethanolamine 1.1
13. Methyl paraoxybenzoate 0.2
14. Titanium dioxide 8.0
15. Talc 4.0
16. Bengala 1.0
17. Yellow iron oxide 2.0
18. Appropriate amount of fragrance 19. [Manufacturing method] Ingredients 2 to 8 having a total amount of 100 in purified water are dissolved by heating and kept at 80 ° C. to prepare an oil phase. Ingredient 19 is well swollen with Ingredient 9, followed by Additions 1 and 10-13 and mixed uniformly. Ingredients 14 to 17 pulverized and mixed by a pulverizer are added thereto, and the mixture is stirred with a homomixer and kept at 75 ° C. to prepare an aqueous phase. The aqueous phase is added to this oil phase while stirring to emulsify. After that, it is cooled, component 18 is added at 45 ° C., and the product is cooled to 30 ° C. with stirring.

Prescription example 10 Bath agent Prescription compounding amount (part)
1. 1. Extract of Production Example 6A 5.0
2. 2. Extract of Production Example 6B 1.0
3. 3. Sodium bicarbonate 50.0
4. Yellow No. 202 (1) Appropriate amount 5. Appropriate amount of fragrance 6. [Manufacturing method] Ingredients 1 to 6 with sodium sulfate to make the total amount 100 are uniformly mixed to prepare a product.

Prescription example 11 Ointment prescription compounding amount (part)
1. 1. Extract of Production Example 4B 0.5
2. 2. Polyoxyethylene cetyl ether (30EO) 2.0
3. 3. Glycerin monostearate 10.0
4. Liquid paraffin 5.0
5. Cetanol 6.0
6. Methyl paraoxybenzoate 0.1
7. Propylene glycol 10.0
8. [Manufacturing method] Ingredients 2 to 5 having a total amount of 100 in purified water are dissolved by heating and mixed, and kept at 70 ° C. to prepare an oil phase. Ingredients 1 and 6 to 8 are heated, dissolved and mixed, and kept at 75 ° C. to prepare an aqueous phase. An aqueous phase is added to the oil phase to emulsify, and the product is cooled to 30 ° C. with stirring.

Prescription example 12 Powder prescription compounding amount (part)
1. 1. Extract of Production Example 4A 20.0
2. 2. Dried cornstarch 30.0
3. 3. Microcrystalline Cellulose 50.0
[Manufacturing method] Ingredients 1 to 3 are mixed to prepare a powder.

Prescription example 13 Tablets Prescription compounding amount (part)
1. 1. Extract of Production Example 4A 3.0
2. 2. Dried cornstarch 27.0
3. 3. Carboxymethyl cellulose calcium 20.0
4. Microcrystalline Cellulose 40.0
5. Polyvinylpyrrolidone 7.0
6. Talc 3.0
[Manufacturing method] Ingredients 1 to 4 are mixed, and then an aqueous solution of ingredient 5 is added as a binder to form granules. Ingredient 6 is added to the molded granules and the mixture is locked. One tablet weighs 0.52 g.

Prescription example 14 Tablet confectionery prescription compounding amount (part)
1. 1. Extract of Production Example 5A 0.5
2. 2. Dried cornstarch 50.0
3. 3. Erythritol 40.0
4. Citric acid 5.0
5. Sucrose fatty acid ester 3.0
6. Appropriate amount of fragrance 7. [Manufacturing method] Ingredients 1 to 4 and 7 having a total amount of 100 are mixed with purified water to form granules. Ingredients 5 and 6 are added to the molded granules and the mixture is locked. One grain is 1.0 g.

Prescription example 15 Beverage prescription compounding amount (part)
1. 1. Extract of Production Example 5A 2.0
2. 2. Fructose-glucose liquid sugar 12.5
3. 3. Citric acid 0.1
4. Fragrance 0.05
5. [Manufacturing method] Ingredients 1 to 5 having a total amount of 100 are mixed with purified water to prepare a beverage.

Prescription example 16 Powdered beverage Prescription compounding amount (part)
1. 1. Extract of Production Example 7A 10.0
2. 2. Powdered sugar 65.0
3. 3. Powdered peach juice 15.0
4. L-ascorbic acid 8.0
5. Crystalline citric acid 1.2
6. Sodium citrate 0.75
7. Aspartame 0.02
8. Powdered peach flavor 0.03
[Manufacturing method] Ingredients 1 to 8 are mixed to prepare a powdered beverage.

Prescription example 17 Herbal tea prescription compounding amount (part)
1. 1. Nasturtium dried product (Table 1 of Example 1 excluding comparative examples) 1.0
2. 2. Peppermint 0.5
3. 3. Rosehip 0.5
[Manufacturing method] Ingredients 1 to 3 are mixed, and 2 g is sealed in a tea bag to make herbal tea.

From the above, nastatium and its extract cultivated by irradiating light having a specific wavelength range show excellent antioxidant effect, matrix metalloproteinase (MMP) inhibitory effect and whitening effect, and the skin containing these. External or internal preparations are particularly effective.

Claims (3)

The total photosynthetic photon flux density (PPFD) of red light in the wavelength range of 570 to 730 nm and blue light of 400 to 515 nm is 50 to 200 μmol · m- ² s ^-1 , and the ratio of red light to blue light is 2: 1. It is a cultivation method of nastathium in which irradiation is performed at a ratio of ~ 1: 1 and cultivation is performed at a cultivation temperature of 20 to 25 ° C. under cultivation conditions for 10 to 30 days, and irradiation is performed with fluorescent light or sunlight having the same photosynthetic photon bundle density. However, a method for cultivating nastathium , which is characterized by having a higher antioxidant effect than nastatium cultivated under the same cultivation conditions . The Nasturtium grown in the described cultivation method in claim 1, water, the extract extracted by one or two or more solvents selected from lower alcohols and liquid polyhydric alcohols containing from 0.001 to 10 wt% A wrinkle formation inhibitor characterized by this. The Nasturtium grown in the described cultivation method in claim 1, water, the extract extracted by one or two or more solvents selected from lower alcohols and liquid polyhydric alcohols containing from 0.001 to 10 wt% An antioxidant characterized by that.