JP6895287B2 - How to produce plant cuttings - Google Patents

Description

The present invention relates to a method for producing plant cuttings.

Cuttings are a method of rooting in the rooting bed using artificially cut plant tissue to produce one independent plant, which is an excellent method for mass growth of genetically uniform seedlings. Is.

Propagation by cuttings is simple, suitable for producing a large number of seedlings at one time, and it is a commercially advantageous method because a large number of individuals with excellent traits can be proliferated. However, there are many plant species that are difficult to root with cuttings.

Non-Patent Document 1 describes that the timing of fertilization in cuttings should be after the growth of buds is resumed. It is also described that the reason is that when the cutting bed has a large amount of fertilizer, water easily escapes from the cut end of the cutting due to osmotic pressure, and the new roots are easily affected by the concentration disorder.

Patent Document 1 describes that by using a cutting bed made by applying fertilizer to sludge compost, which is an aerobic fermentation product of organic sludge, fertilization obstacles at the time of insertion are suppressed and the rooting rate is increased. ..

JP-A-2010-124743

Hiroo Koike, "Grafted Fruit Trees, Cuttings, Layered Trees", Rural Culture Association Japan, February 20, 2010, 7th edition issued

However, even with the method of Patent Document 1, there is a problem that fertilizer application management is difficult because the appropriate amount of fertilizer applied at the time of insertion differs depending on the cultivation conditions such as the type of fertilizer, plant species, temperature, humidity, and pH. An object of the present invention is to provide a method for producing plant cuttings, which can increase the efficiency of seedling production by cuttings.

The present invention provides the following [1] to [4].
[1] In a method for producing plant cuttings, which includes a rooting culture step in which cuttings are inserted into a rooting medium and rooting culture is performed, and a seedling raising step in which the cuttings after rooting are raised in a seedling raising medium.
The electrical conductivity in the rooting medium was controlled to exceed 0.01 mS / cm and less than 0.5 mS / cm.
A method of controlling the electrical conductivity in a seedling raising medium to 0.5 mS / cm or more.
[2] The method of [1], wherein the difference between the electrical conductivity in the rooting medium and the electrical conductivity in the seedling raising medium is 0.05 to 0.6 mS / cm.
[3] The method of [1] or [2], wherein the plant is a plant of the genus Sugi, a plant of the genus Pine or a plant of the genus Eucalyptus.
[4] The method according to any one of [1] to [3], which uses a rooting promoter in the rooting culture step.

According to the present invention, the efficiency of seedling production by cuttings can be increased. Specifically, it is possible to increase the efficiency of seedling production by cuttings with a better seedling acquisition rate than in the case of rooting culture without fertilizer and topdressing at the time of raising seedlings. Further, by controlling the electric conductivity (also referred to as "EC value"), it is possible to appropriately control the amount of fertilizer applied to the medium for increasing the efficiency of seedling production regardless of the type of fertilizer.

The present invention provides a method for producing plant cuttings, which includes a rooting culture step and a seedling raising step.

[Rooting culture process]
In the rooting culture step, cuttings are inserted into a rooting medium for rooting culture.

(Cutting)
The cuttings may be the cuttings of the plant for which cutting seedlings are to be obtained. The type of plant is not particularly limited. Plants can be classified into woody plants and herbaceous plants, but this plant can be applied to any of these, preferably applied to woody plants, and has inferior rooting ability to herbaceous plants. More preferably, it is applied to woody plants. As woody plants, Eucalyptus plants, Pinus plants, Cryptomeria plants (Cryptomeria japonica, etc.), Sakura genus (Prunus spp.), Ume (Prunus mume), Yusuraume (Prunus tomentosa, etc.), Mangofera plants (Mangifera indica, etc.), Acacia plants, Yamamomo (Myrica) plants, Kunugi (Qu) Etc. (Quarcus actissima), grapes (Vitis), apples (Marus), roses (Rosa), camellia plants (Camellia sinensis, etc.), Jacaranda plants (Jacaraanda) Jacaranda (Jacaranda mimosifolia, etc.), crocodile (Persea) plants (Avocado (Persea americana), etc.), Pear (Pyrus) plants (Pear (Pyrus serotina Rehder, Pyrus) (Sandalum album, etc.) and the like) are exemplified. Of these, the effect of the present invention is more exhibited when applied to eucalyptus, pine, cedar, cherry, mango, avocado, acacia, bayberry, kunugi, grape, apple, rose, camellia, tea, plum, tomentosa, jacaranta, etc. Can be done. Among them, eucalyptus plants, pine plants, sugi genus plants, camellia genus plants, mango genus plants, and crocodile genus plants are preferable, eucalyptus genus plants, pine genus plants, and sugi genus plants are more preferable, and sugi genus plants are further preferable.

The cuttings may be at least a part of the plant, and branches such as green branches (current year branches) and mature branches (branches that grew before the previous year); shoots such as apical buds and axillary buds; leaves, cotyledons; hypocotyls, etc. Is illustrated. The cuttings in the case of woody plants are usually green or mature branches, and the cuttings in the case of herbaceous plants are usually leaves or buds, but are not limited thereto.

Shoots may be used as cuttings from the viewpoint that they are expected to form adventitious roots. The shoot refers to all tissues having rooting ability. Examples of the tissue include branches, stems, apical buds, axillary buds, adventitious buds, leaves, cotyledons, hypocotyls, adventitious embryos, shoot primordia and the like. The origin of the shoot is not particularly limited, and it may be a tissue obtained from an individual plant growing in a greenhouse or outdoors, a cultured tissue obtained by a tissue culture method, or a part of a natural plant body. It may be an organization of. Shoots can be efficiently obtained from the mother plant of cuttings or polyblasts. Among them, cuttings (cuttings obtained from the mother plant), polyblasts obtained by aseptically culturing an organ collected from the mother plant, or foliage obtained by aseptically growing the organ. It is preferable to have.

The multi-bud can be derived by cutting tissues such as apical buds and axillary buds from a plant for which the present invention is to be produced to produce cloned seedlings, and tissue culturing the tissues. The polybuds can be formed by aseptically culturing an organ collected from a mother plant according to the method and conditions described in JP-A-8-228621. The method and conditions are as follows. First, tissues such as apical buds and axillary buds are collected from the plant as a material, and the collected tissues are subjected to an aqueous solution of sodium hypochlorite having an effective chlorine content of about 0.5% to about 4% or an effective chlorine content of about 5% to. Surface sterilization is performed by immersing in an aqueous solution of hydrogen peroxide of about 15% for about 10 minutes to about 20 minutes. Then, this is washed with sterile water, inserted into a solid medium to open the buds, and the elongated foliage is subcultured in a medium having the same composition to form polyblasts. When a tissue of the genus Eucalyptus or the genus Acacia (for example, axillary bud) is used, the solid medium contains 1 to 5% by weight of sucrose, and benzyladenine (hereinafter abbreviated as BA) as a plant hormone is about 0.02 mg / L or more. Murashige sucrose (hereinafter abbreviated as MS) medium containing 1 mg / L or less, about 0.2% by weight or more of gellan gum or about 0.3% by weight or about 0.5% by weight or more of adenine or about 1% by weight or less, or MS. It is preferable to use a modified MS medium in which the ammonium nitrate component and the potassium nitrate component of the medium are halved. Shoots actively grow from the polyblasts formed in this way. The polyblast itself can be maintained and proliferated by appropriately dividing and culturing in a medium having the same composition as the medium used for polyblast formation.

(EC value of rooting medium)
In the rooting culture step, the electrical conductivity (also referred to as “EC value”) in the rooting medium is set to less than 0.5 mS / cm, preferably 0.49 mS / cm or less, more preferably 0.48 mS / cm or less. Control. As a result, an excellent seedling acquisition rate can be obtained as compared with the case of rooting culture without fertilization and topdressing at the time of raising seedlings, and the amount of fertilizer applied during rooting culture can be adjusted to an appropriate range. The lower limit may exceed 0.01 mS / cm, preferably 0.02 mS / cm or more, 0.05 mS / cm or more, 0.15 mS / cm or more, or 0.20 mS / cm or more. The electrical conductivity means the ease of charge flow and can be an index of the ion concentration in the medium. The method for measuring the electrical conductivity is not particularly limited, but the medium may be measured directly or indirectly (for example, after dilution with water or the like). The measuring device is not particularly limited, and examples thereof include a portable EC measuring device. The EC value of the rooting medium may be measured and confirmed periodically during the rooting culture process, but since it usually decreases with the lapse of the rooting culture period, rooting at the start of rooting culture If it can be confirmed that the EC value of the medium is less than 0.5 mS / cm, the subsequent confirmation work can be omitted. Examples of the method for controlling the electrical conductivity in the rooting medium include addition of fertilizer components, temperature adjustment, pH adjustment and the like.

(Rooting medium)
The rooting medium is not particularly limited, and for example, a cultivation medium such as a nutrient solution, hydroponic cultivation water, a plant tissue culture medium, a diluted medium of the cultivation medium, or a medium in which a fertilizer component is appropriately added to the cultivation medium. Can be mentioned. The rooting culture step may be carried out by, for example, hydroponics or soil cultivation.

Examples of the fertilizer component include components that can be a source of plant nutrients such as inorganic components, silver ions, antioxidants, carbon sources, vitamins, amino acids, and plant hormones. The form of the fertilizer component is not particularly limited, and may be either a solid substance (eg, powder, granule) or a liquid (eg, liquid fertilizer).

Examples of the inorganic component include elements such as nitrogen, phosphorus, potassium, sulfur, calcium, magnesium, iron, manganese, zinc, boron, molybdenum, chlorine, iodine and cobalt, and inorganic salts containing these elements. Examples of the inorganic salt include potassium nitrate, ammonium nitrate, ammonium chloride, sodium nitrate, potassium monohydrogen phosphate, sodium dihydrogen phosphate, potassium chloride, magnesium sulfate, ferrous sulfate, ferric sulfate, manganese sulfate, and zinc sulfate. , Copper sulfate, sodium sulfate, calcium chloride, magnesium chloride, boric acid, molybdenum trioxide, sodium molybdate, potassium nitrate, cobalt chloride and the like, and hydrates thereof. As the inorganic component, one type may be selected from the above specific examples, or two or more types may be used in combination. The liquid medium used in the present invention preferably contains nitrogen, phosphorus and potassium as essential elements. Therefore, among the specific examples of these inorganic components, nitrogen, phosphorus, potassium, an inorganic salt containing nitrogen, an inorganic salt containing phosphorus, and an inorganic salt containing potassium are preferable, and an inorganic salt containing nitrogen, phosphorus, potassium, and nitrogen is preferable. More preferred. The inorganic component is preferably added so that the concentration in the liquid medium is about 1 μM to about 100 mM in the case of one kind, and more preferably about 0.1 μM to about 100 mM. In the case of a combination of two or more kinds, it is preferable to add them so as to be about 0.1 μM to about 100 mM, and more preferably to add them so as to be about 1 μM to about 100 mM.

Examples of the silver ion include silver compounds (silver ion source) such as silver _{thiosulfate (STS, AgS 4} O _{6) and silver nitrate, and STS is preferable.} STS takes the form of silver thiosulfate ions in the medium and is presumed to be negatively charged, which can contribute to the promotion of healthy root rooting and elongation. The concentration of silver ions added to the medium depends on the type of silver ion source and other culture conditions, but the concentration of the silver ion source is preferably about 0.5 μM or more and about 6 μM or less, and about 2 μM or more and about 6 μM or less. More preferred.

Examples of the antioxidant include ascorbic acid and sulfite, and ascorbic acid is preferable. Since ascorbic acid has low persistence in the medium, environmental pollution can be suppressed. The concentration of the antioxidant added to the medium is preferably about 5 mg / l or more and about 200 mg / l or less, and more preferably about 20 mg / l or more and about 100 mg / l or less.

As the carbon source, compounds such as carbohydrates such as sucrose and derivatives thereof; organic acids such as fatty acids; and primary alcohols such as ethanol can be used. As the carbon source, one type may be selected from the above specific examples, or two or more types may be used in combination. The carbon source is preferably added to the liquid medium in an amount of about 1 g / l to about 100 g / l, and more preferably to an amount of about 10 g / l to about 100 g / l. However, when the cultivation is carried out while supplying carbon dioxide gas, the medium does not need to contain a carbon source and is preferably not contained. Organic compounds that can be a carbon source such as sucrose also serve as a carbon source for microorganisms. Therefore, when using a medium containing these, it is necessary to cultivate in a sterile environment, but a medium that does not contain a carbon source is used. This enables cultivation in a non-sterile environment.

As vitamins, for example, biotin, thiamine (vitamin B1), pyridoxine (vitamin B4), pyridoxal, pyridoxamine, calcium pantothenate, inositol, nicotinic acid, nicotinamide and / or riboflavin (vitamin B2) and the like should be used. Can be done. As the vitamins, one type may be selected from the above specific examples, or two or more types may be used in combination. The vitamins are preferably added to the liquid medium so that the concentration in the liquid medium is about 0.01 mg / l to about 200 mg / l, and about 0.02 mg / l to about 100 mg. It is more preferable to add it so as to be / l. In the case of a combination of two or more, it is preferable to add them to the liquid medium so as to be about 0.01 mg / l to about 150 mg / l, and to add them to about 0.02 mg / l to about 100 mg / l, respectively. It is more preferable to do so.

As the amino acids, for example, glycine, alanine, glutamic acid, cysteine, phenylalanine and / or lysine can be used. As the amino acids, one type may be selected from the above specific examples, or two or more types may be used in combination. Amino acids are preferably added to the liquid medium so that the concentration in the liquid medium is about 0.1 mg / l to about 1000 mg / l in the case of one type, and in the case of a combination of two or more types, the amino acids are preferably added. It is preferable to add each to a liquid medium so as to have a concentration of about 0.2 mg / l to about 1000 mg / l.
Examples of plant hormones include rooting promoters such as auxin and cytokinin. Examples of auxins include naphthalene acetic acid (NAA), indoleacetic acid (IAA), p-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid (2,4D), indolebutyric acid (IBA) and derivatives thereof. One or more or a combination of two or more selected from the above can be used. Examples of cytokinins include benzyladenine (BA), kinetin, zeatin and derivatives thereof, and one or more or a combination of two or more selected from these can be used. The plant hormone is preferably auxin or a combination of auxin and cytokinin.

The concentration of the plant hormone in the rooting medium is preferably 0.001 mg / l to 10 mg / l, and more preferably 0.01 mg / l to 10 mg / l when one type of plant hormone is used. preferable. When there are two or more plant hormones, the amount is preferably 0.001 mg / l to 10 mg / l, and more preferably 0.01 mg / l to 10 mg / l, respectively. The method of adding the plant hormone is not particularly limited, and it may be added according to the instruction manual of a commercially available product. Examples thereof include a method of applying the plant hormone as it is to the base of cuttings and a method of adding it to a medium.

The nutrient solution, hydroponic cultivation water, and medium for plant tissue culture may be appropriately selected from known ones and diluted as necessary. Examples of the medium for culturing plant tissues include MS (Murashige-Skoog) medium, Rinsemeier Skoog medium, White medium, Gambog B-5 medium, Nitch Nitch medium and the like. Of these, MS medium and Ganborg's B-5 medium are preferable.

The rooting medium may include a support. Thereby, the cuttings can be supported in the rooting medium and the rooting culture can be efficiently carried out. The support is not particularly limited as long as the cuttings can be held in a supported state during the rooting culture step, and a conventional support can be used. As the support, for example, natural soil such as sand and soil (eg, Akadama soil) (preferably Akadama soil); artificial soil such as paddy husk charcoal, coconut fiber, vermiculite, pearlite, peat moss, glass beads; , Porous molded products such as rock wool; solidifying agents (eg, agar or gellan gum) and the like. The support may be any one that does not interfere with the contact between the cuttings and the medium, and the support may contain at least a part of the medium.
The medium and support may be placed in a container. The container is not particularly limited, but a container having a structure divided so that a plurality of cuttings can be processed one by one is preferable. When the cuttings are branches, it is preferable to use a closed culture vessel. As a result, the cuttings can be easily placed under high humidity, so that the transpiration of the leaves attached to the branches can be suppressed, and the conventional partial excision of the leaves can be omitted.
The method of placing the rooting medium in the container is not particularly limited, and for example, a method of putting the support in the container and then putting the medium, a method of putting the medium in the container and then putting the support in the container, and a method of putting the support in the container. Examples thereof include a method of holding the medium in a container and a method of inflating the support with the medium in advance and placing the swollen support in a container.

In the rooting step, usually one kind of rooting medium is used, but a combination of two or more kinds of rooting media may be used by dividing the period or the like.

In the rooting culture step, the method of inserting the cuttings into the rooting medium may be appropriately selected depending on the type of medium, the culture conditions and the like. It is also preferable to apply a physical stimulus such as scratching the base of the cutting when the rooting medium is inserted to improve the rooting rate. The base of the cutting means the region at one end of the cutting where the root is formed (opposite to the end where the leaf is formed). When a multi-bud is used as a cutting, the base is a region having a cut surface when the multi-bud is divided. The size (size, shape, etc.) of the wound on the base of the cutting is not particularly limited. For example, when a polyblast is used as the cutting, it is preferable to scratch the base of the cutting (the cut surface described above) so as to form a cross when viewed from the front. When scratching, tools such as scissors and knives can be used.

(Culture place)
The place where the rooting culture step is carried out may be a closed space (eg, in a vinyl house, a carbon dioxide gas culture room, indoors) or an open space (eg, outdoors), but a closed space is preferable. This makes it easy to adjust seedling raising conditions such as temperature and humidity.

The culture period of the rooting culture step varies depending on the plant species, but is usually 2 weeks to 3 months, preferably 4 weeks to 2 months. The rooting culture step may be continued until rooting is observed from the cuttings.

[Seedling raising process]
In the seedling raising process, the cuttings after rooting are raised in a seedling raising medium.

The cuttings after rooting are cuttings that have been rooted through a rooting culture step. The rooting of the cuttings should be confirmed by visual observation.

(EC value of seedling medium)
In the seedling raising step, the EC value in the seedling raising medium is controlled to 0.5 mS / cm or more, preferably 0.53 mS / cm or more, more preferably 0.55 mS / cm or more, still more preferably 0.58 mS / cm or more. .. As a result, a sufficient amount of fertilizer can be applied and the growth of cuttings can be efficiently promoted. The upper limit is not particularly limited, and is usually 100 mS / cm or less, 50 mS / cm or less, 10 mS / cm or less, or 5 mS / cm or less. The EC value measuring method and measuring device are as described in the item of EC value of the rooting medium. The EC value of the seedling raising medium may be controlled by a method such as adding a fertilizer component, adjusting the temperature, or adjusting the pH while measuring it regularly.

The difference between the EC value in the rooting medium and the EC value in the seedling raising medium is preferably 0.05 mS / cm or more, more preferably 0.07 mS / cm or more, and even more preferably 0.1 mS / cm or more. The upper limit is preferably 0.6 mS / cm or less, more preferably 0.55 mS / cm or less, and even more preferably 0.5 mS / cm or less. As a result, cuttings can be efficiently produced.

(Seedling medium)
The example of the seedling raising medium is the same as the example of the rooting medium described in the item of rooting medium. The rooting medium and the seedling raising medium may be common or different, but are preferably common with the rooting medium, and the rooting medium may be used as it is as the seedling raising medium. That is, the seedling raising step may be continuously carried out using the rooting medium as the seedling raising medium while the cuttings after rooting are inserted into the rooting medium. On the other hand, the seedling raising step may be carried out by replacing the cuttings after rooting with a seedling raising medium prepared separately from the rooting medium. The seedling raising step may be carried out by, for example, hydroponics or soil cultivation.

(Nursery place)
The place where the seedling raising step is carried out may be a closed space (eg, in a vinyl house, a carbon dioxide gas culture room, indoors) or an open space (eg, outdoors), but a closed space is preferable. This makes it easy to adjust seedling raising conditions such as temperature and humidity. It is preferable that the rooting culture step and the seedling raising step are carried out at the same place.
In the rooting culture step, the seedling raising step may be carried out immediately after rooting, but the acclimatization step may be carried out after the rooting culture step is completed and before the seedling raising step is carried out. As a result, when the cuttings are shoots derived from cultured tissues such as adventitious shoots and seedling primordia, seedlings can be raised efficiently.

[Cultivation conditions]
The cultivation conditions for cultivating a plant are not particularly limited as long as the conditions allow rooting from the plant. It is difficult to unconditionally define the cultivation conditions depending on the type, site, condition, type of rooting medium, etc. of the plant, but an example will be described below.

(temperature)
The temperatures of the rooting medium and the seedling raising medium are usually 20 ° C. to 30 ° C., preferably 22 ° C. to 26 ° C., and may be different or the same within the above range. The temperature of the cultivation environment in the rooting culture step and the seedling raising step is preferably 20 ° C. or higher, more preferably 22 ° C. or higher, and even more preferably 23 ° C. or higher. The upper limit of the temperature of the cultivation environment in the rooting culture step and the seedling raising step is preferably 30 ° C. or lower, more preferably 28 ° C. or lower, and even more preferably 26 ° C. or lower. The temperature of the cultivation environment in the rooting culture step and the seedling raising step may be different or may be the same.

(PH of medium)
The pH of the rooting medium and the seedling raising medium is preferably 4 to 8, and more preferably about pH 4 (for example, pH 4 to 6). This makes it possible to suppress the growth of germs and the like. The pH of the rooting medium and the seedling raising medium may be different or may be the same.

(Amount of light)
The light irradiation conditions are not particularly limited, and sunlight may be used or artificial light may be used. The light intensity is not particularly limited, photosynthetic expressed as photon flux density is preferably not more than about 10μmol / m ² / s or more to about 1000 micro mol / m ² / s, about 50μmol / m ² / s or more to about 500 [mu] mol / More preferably, it is m ^{2 / s or less.}

The light wavelength is not particularly limited, but it is preferable to carry out the irradiation under irradiation of light containing a wavelength component of about 650 nm or more and about 670 nm or less and a wavelength component of about 450 nm or more and about 470 nm or less in a ratio of 9: 1 to 7: 3. It is more preferable to carry out the irradiation under irradiation of light containing these wavelength components at a ratio of 9: 1 to 8: 2. Rooting from plants can be further promoted by cultivating by irradiating light containing such a wavelength component.

In the rooting step, it is preferable to perform shading. The shading rate is preferably 30% or more and 70% or less, and more preferably 40% or more and 60% or less.

(Carbon dioxide concentration)
The carbon dioxide gas in the cultivation environment is usually preferably supplied so as to be 300 ppm or more and 2000 ppm or less, preferably 800 ppm or more and 1500 ppm or less. The amount of carbon dioxide supplied can be controlled by using equipment such as an artificial meteorological instrument or a culture vessel having a carbon dioxide permeable membrane at the opening.

Humidity can be adjusted according to cultivation conditions such as the type of plant, but is usually 50% or more, preferably 60% or more. As a result, rooting from plants can be promoted and seedlings can be raised efficiently. There is no particular limit on the upper limit.

Hereinafter, the present invention will be described with reference to examples, but the present invention is not limited thereto.

(Example 1)
A 20 cm apical bud branch to be a cutting was collected from a perennial branch of Sugi (Tanoaka; Cryptomeria japonica), and all the leaves in the lower 5 cm range were cut to prepare cuttings. Using a cell tray as a culture container, Akatama small-grain soil (manufactured by Yasushima Shoji Co., Ltd.) and peat moss (manufactured by Toho Co., Ltd.) are mixed 1: 1 and a commercially available chemical fertilizer (powder: trade name "Amino House") is used as the base fertilizer. No. 1 ”(manufactured by Otsuka Agrio Techno Co., Ltd.) was added at a ratio of 5 g / L and mixed, and water was added to these to prepare a medium. The mixed medium was filled in a culture vessel (cell tray) and inserted to prepare a bed. After preparing the cutting bed, the electrical conductivity (hereinafter referred to as EC value) of the medium was measured using a soil direct EC / ° C tester HI 98331N (Hanna Instruments Japan Co., Ltd.). Luton (registered trademark) (manufactured by Ishihara Bioscience Co., Ltd., a white powder containing the plant hormone α-naphthylacetamide (NAA)) at the base (cut portion) of the cuttings prepared as described above, the concentration of NAA is 0. After applying 5 to 10 mg of the powder of 4%), the cuttings were inserted up to 4 to 5 cm from the base and inserted into the floor. The cuttings were placed in a plastic greenhouse and rooted and cultured for 2 months. The cuttings after culturing were observed with the naked eye, and if roots were confirmed, it was judged that the roots had rooted.
After rooting culture, seedlings were continuously raised in a plastic greenhouse for 4 months. Chemical fertilizer (liquid fertilizer) diluted while measuring the EC value to 0.5 to 1.0 (mS / cm) during seedling raising (liquid agent: trade name "Hyponex undiluted solution" (manufactured by Hyponex Japan Co., Ltd.)) Was sprayed once a week to add fertilizer. In addition, the EC value immediately after topdressing was measured. The seedlings after raising the seedlings were observed with the naked eye, and it was judged that the seedlings were obtained if they were longer than the ears at the time of insertion and were alive. EC value, number of roots, rooting rate ((number of roots / number of cuttings) x 100), number of seedlings, rate of seedlings ((number of seedlings / number of roots) x 100), and growth amount (seedling height) ) Is shown in Table 1. The test was carried out in a vinyl house, and no particular adjustment was made except for the above-mentioned conditions. However, throughout the test, the pH of the medium during culturing was about 6, the carbon dioxide concentration was a natural value (about 400 ppm), and light. The temperature was around 25 ° C. and the humidity was around 60%, using sunlight instead of artificial light.

(Example 2)
The same procedure as in Example 1 was carried out except that a commercially available chemical fertilizer (powder) was added as a base fertilizer at a ratio of 2.5 g / L and mixed.

(Comparative Example 1)
The procedure was carried out in the same manner as in Example 1 except that no basal fertilizer was added.

(Comparative Example 2)
The same procedure as in Example 1 was carried out except that a commercially available chemical fertilizer (powder) was added as a base fertilizer at a ratio of 10 g / L and mixed.

(Comparative Example 3)
After rooting culture, it was carried out in the same manner as in Example 2 except that no additional fertilizer was applied.

As shown in Table 1, the rooting rate (survival rate) of Examples 1 and 2 was higher than that of Comparative Example 2. From this result, according to the present invention, by controlling the EC value to exceed 0.01 (mS / cm) and less than 0.5 (mS / cm), rooting culture can be performed so as not to deplete the cuttings. It turned out that.
In addition, the seedling acquisition rate (survival rate) of Examples 1 and 2 was higher and the amount of growth was larger than that of Comparative Examples 1 to 3. From this result, the EC value of the medium was controlled to exceed 0.01 (mS / cm) and less than 0.5 (mS / cm) during rooting culture, and the EC value of the medium was controlled to 0.5 (mS / cm) during seedling raising. It was found that by controlling the value above / cm), it is possible to improve the seedling yield rate (survival rate) and the growth amount (seedling height) as compared with the case where the EC value is not controlled within this range.

(Example 3)
As a cutting, instead of a cedar, a 20 cm apical bud branch to be a cutting was collected from a perennial branch of a black pine (Pinus sunbergii), and all the leaves in the lower 5 cm range were cut to prepare a cutting, as a basal fertilizer. Commercially available organic fertilizer (compost: trade name "High Power Compost" (manufactured by Asahi Kogyo Co., Ltd.)) was added at a ratio of 25 g / L and mixed to prepare a medium (rooting medium). Immerse the base (cut part) of the ear in Oxyberon (registered trademark) solution (manufactured by Bayer Crop Science Co., Ltd., plant hormone containing indol-3-butyric acid (IBA), plant hormone concentration 0.4%) for 10 seconds. What was done, the cuttings were placed in a carbon dioxide gas culture room (supplying carbon dioxide gas to 1000 ppm) instead of the vinyl greenhouse, and rooting culture was performed for 2 months, acclimatization (25 ° C, humidity 60-80). %, 14 days), but the same procedure as in Example 1 was carried out. Table 2 shows the EC value, the number of roots, the rooting rate (number of roots / number of cuttings), the number of seedlings obtained, the rate of seedlings obtained (number of seedlings / number of roots), and the amount of growth (seedling height).

(Example 4)
The same procedure as in Example 3 was carried out except that a commercially available organic fertilizer (compost) was added as a basal fertilizer at a ratio of 12.5 g / L and mixed.

(Comparative Example 4)
It was carried out in the same manner as in Example 3 except that the basal fertilizer was not added.

(Comparative Example 5)
The same procedure as in Example 3 was carried out except that a commercially available organic fertilizer (compost) was added as a basal fertilizer at a ratio of 50 g / L and mixed.

As shown in Table 2, the rooting rate (survival rate) of Examples 3 and 4 was higher than that of Comparative Example 5. From this result, according to the present invention, by controlling the EC value to exceed 0.01 m (S / cm) and less than 0.5 (mS / cm), rooting culture can be performed so as not to deplete the cuttings. I understood.
In addition, the seedling acquisition rate (survival rate) of Examples 3 and 4 was higher than that of Comparative Example 4. From this result, the EC value of the medium was controlled to exceed 0.01 m (S / cm) and less than 0.5 (mS / cm) during rooting culture, and the EC value of the medium was set to 0.5 (mS) during seedling raising. It was found that by controlling the value above / cm), it is possible to improve the seedling yield rate (survival rate) and the growth amount (seedling height) as compared with the case where the EC value is not controlled within this range.

(Example 5)
As cuttings, 10 cm apical buds to be cuttings were collected from the current year's branches of Eucalyptus albida instead of black pine, and all the leaves in the lower 2 cm range were cut to adjust the cuttings, basal fertilizer. A commercially available chemical fertilizer (powder) was added at a ratio of 5 g / L and mixed to prepare a medium (rooting medium). The same procedure as in Example 3 was carried out except that the cuttings were attached and the cuttings were placed in a carbon dioxide gas culture chamber and rooted and cultured for 1 month. Table 3 shows the EC value, the number of roots, the rooting rate (number of roots / number of cuttings), the number of seedlings obtained, the rate of seedlings obtained (number of seedlings / number of roots), and the amount of growth (seedling height).

(Example 6)
The same procedure as in Example 5 was carried out except that a commercially available chemical fertilizer (powder) was added (× 1/4 amount) as a basal fertilizer at a ratio of 2.5 g / L and mixed.

(Comparative Example 6)
The procedure was carried out in the same manner as in Example 5 except that no basal fertilizer was added.

(Comparative Example 7)
The same procedure as in Example 5 was carried out except that a commercially available chemical fertilizer (powder) was added (x1 amount) as a base fertilizer at a ratio of 10 g / L and mixed.

As shown in Table 3, the rooting rate (survival rate) of Examples 5 and 6 was higher than that of Comparative Example 7. From this result, according to the present invention, by controlling the EC value to exceed 0.01 m (S / cm) and less than 0.5 (mS / cm), rooting culture can be performed so as not to deplete the cuttings. I understood.
In addition, the seedling acquisition rate (survival rate) of Examples 5 and 6 was higher than that of Comparative Example 6. From this result, the EC value of the medium was controlled to exceed 0.01 m (S / cm) and less than 0.5 (mS / cm) during rooting culture, and the EC value of the medium was set to 0.5 (mS) during seedling raising. It was found that by controlling the value above / cm), it is possible to improve the seedling yield rate (survival rate) and the growth amount (seedling height) as compared with the case where the EC value is not controlled within this range.

Claims (3)

In a method for producing cuttings of a plant, which includes a rooting culture step in which cuttings are inserted into a rooting medium and rooting culture is performed, and a seedling raising step in which the cuttings after rooting are raised in a seedling raising medium.
In the rooting culture step, the electrical conductivity in the rooting medium is controlled to be 0.05 mS / cm or more and 0.49 mS / cm or less.
In the seedling raising step, the electric conductivity in the seedling raising medium is 0.5 mS / cm or more and 0.75 mS / cm or less, and the difference from the electric conductivity in the rooting medium is 0.1 to 0.5 mS / cm. controlled in such a way that,
The plant is a woody plant,
Method. The method according to claim 1 , wherein the plant is a plant of the genus Sugi, a plant of the genus Pine, or a plant of the genus Eucalyptus. The method according to claim 1 or 2 , wherein a rooting promoter is used in the rooting culture step.