JP6687549B2 - Ｌ−トリプトファン生産能を有するエシェリキア属微生物及びこれを用いたｌ−トリプトファンの製造方法 - Google Patents

Ｌ−トリプトファン生産能を有するエシェリキア属微生物及びこれを用いたｌ−トリプトファンの製造方法 Download PDF

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Publication number: JP6687549B2
Authority: JP; Japan
Prior art keywords: tryptophan; microorganism; gene; phosphogluconate; escherichia
Prior art date: 2014-06-23
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Active

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JP2016575078A

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English (en)

Japanese (ja)

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JP2017518759A (ja

Inventor

ヨンチョン、キ

リムキム、キョン

ミョンイ、ソク

ホイ、グァン

チョルイ、クン

ピョホン、ヒョン

Original Assignee

シージェイチェイルジェダングコーポレイション

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2014-06-23

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2015-06-23

Publication date

2020-04-22

2015-06-23 Application filed by シージェイチェイルジェダングコーポレイション, シージェイチェイルジェダングコーポレイション filed Critical シージェイチェイルジェダングコーポレイション

2017-07-13 Publication of JP2017518759A publication Critical patent/JP2017518759A/ja

2020-04-22 Application granted granted Critical

2020-04-22 Publication of JP6687549B2 publication Critical patent/JP6687549B2/ja

Status Active legal-status Critical Current

2035-06-23 Anticipated expiration legal-status Critical

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QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 title claims description 86
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IFGCUJZIWBUILZ-UHFFFAOYSA-N sodium 2-[[2-[[hydroxy-(3,4,5-trihydroxy-6-methyloxan-2-yl)oxyphosphoryl]amino]-4-methylpentanoyl]amino]-3-(1H-indol-3-yl)propanoic acid Chemical compound [Na+].C=1NC2=CC=CC=C2C=1CC(C(O)=O)NC(=O)C(CC(C)C)NP(O)(=O)OC1OC(C)C(O)C(O)C1O IFGCUJZIWBUILZ-UHFFFAOYSA-N 0.000 claims description 5
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GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
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BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
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ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
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235000013402 health food Nutrition 0.000 description 1
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PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
230000001939 inductive effect Effects 0.000 description 1
238000001802 infusion Methods 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 description 1
229910000358 iron sulfate Inorganic materials 0.000 description 1
BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
239000004310 lactic acid Substances 0.000 description 1
235000014655 lactic acid Nutrition 0.000 description 1
235000018977 lysine Nutrition 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
229940099596 manganese sulfate Drugs 0.000 description 1
239000011702 manganese sulphate Substances 0.000 description 1
235000007079 manganese sulphate Nutrition 0.000 description 1
SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
239000000594 mannitol Substances 0.000 description 1
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235000015097 nutrients Nutrition 0.000 description 1
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230000008569 process Effects 0.000 description 1
238000000746 purification Methods 0.000 description 1
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238000011084 recovery Methods 0.000 description 1
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238000010839 reverse transcription Methods 0.000 description 1
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238000012216 screening Methods 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
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239000000600 sorbitol Substances 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
239000000758 substrate Substances 0.000 description 1
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101150044170 trpE gene Proteins 0.000 description 1
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Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C12P13/227—Tryptophan
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/02—Aldehyde-lyases (4.1.2)
- C12Y401/02014—2-Dehydro-3-deoxy-phosphogluconate aldolase (4.1.2.14)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/01—Hydro-lyases (4.2.1)
- C12Y402/01012—Phosphogluconate dehydratase (4.2.1.12)

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Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
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JP2016575078A 2014-06-23 2015-06-23 Ｌ−トリプトファン生産能を有するエシェリキア属微生物及びこれを用いたｌ−トリプトファンの製造方法 Active JP6687549B2 (ja)

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KR1020140076698A KR101835173B1 (ko)	2014-06-23	2014-06-23	L-트립토판 생산능을 갖는 에스케리키아속 미생물 및 이를 이용한 l-트립토판의 제조 방법
KR10-2014-0076698		2014-06-23
PCT/KR2015/006350 WO2015199406A1 (ko)	2014-06-23	2015-06-23	L-트립토판 생산능을 갖는 에스케리키아속 미생물 및 이를 이용한 l-트립토판의 제조 방법

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JP2018207937A Pending JP2019013256A (ja)	2014-06-23	2018-11-05	Ｌ−トリプトファン生産能を有するエシェリキア属微生物及びこれを用いたｌ−トリプトファンの製造方法

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US (1)	US10815510B2 (es)
EP (1)	EP3159401B1 (es)
JP (2)	JP6687549B2 (es)
KR (1)	KR101835173B1 (es)
CN (1)	CN106574239B (es)
BR (1)	BR112016030467B1 (es)
DK (1)	DK3159401T3 (es)
ES (1)	ES2918459T3 (es)
MY (1)	MY172369A (es)
RU (1)	RU2678139C2 (es)
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MX2020009840A (es)	2018-03-23	2020-10-14	Cj Cheiljedang Corp	Granulos que comprenden l-aminoacido y procedimiento de preparacion de los mismos.
KR101996767B1 (ko) *	2018-11-29	2019-07-04	씨제이제일제당 (주)	cAMP 수용 단백질 변이체 및 이를 이용한 L-아미노산 제조방법
KR102134418B1 (ko) *	2019-06-17	2020-07-16	씨제이제일제당 주식회사	L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법
EP3963067A1 (en) *	2019-07-11	2022-03-09	BP Corporation North America Inc.	Gluconate dehydratase enzymes and recombinant cells
CN115261365B (zh) *	2022-06-22	2024-01-30	南通紫琅生物医药科技有限公司	色氨酸合成酶突变体及其应用

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US913A (en) *		1838-09-12		Improved machine for removing snags and sawyers from the beds of rivers
US4371614A (en) *	1980-08-22	1983-02-01	Ajinomoto Co., Inc.	E.Coli bacteria carrying recombinant plasmids and their use in the fermentative production of L-tryptophan
JP3932945B2 (ja) *	2002-03-27	2007-06-20	味の素株式会社	Ｌ−アミノ酸の製造法
JP2004166595A (ja)	2002-11-20	2004-06-17	Ajinomoto Co Inc	メチロトローフを用いたｌ−アミノ酸の製造法
RU2004105179A (ru)	2004-02-25	2005-08-10	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU)	6-фосфоглюконолактоназа из escherichia coli, фрагмент днк, бактерия, принадлежащая к роду escherichia, -продуцент l-аминокислоты, и способ получения l-аминокислоты
US20050191684A1 (en) *	2004-02-25	2005-09-01	Zimenkov Danila V.	Method for producing L-amino acids
EP1718731B2 (en)	2004-02-25	2016-10-19	Ajinomoto Co., Inc.	Microorganism expressing 6-phosphogluconolactonase and its use in the production of L-amino acids.
RU2288268C2 (ru)	2005-01-26	2006-11-27	Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ)	6-фосфоглюконолактоназа из escherichia coli, фрагмент днк, бактерия, принадлежащая к роду escherichia - продуцент l-аминокислоты и способ получения l-аминокислоты
KR100792095B1 (ko) *	2006-12-29	2008-01-04	씨제이 주식회사	Ｌ-페닐알라닌 생산능을 갖는 대장균 변이주로부터유전자조작된 ｌ-트립토판 생산능을 갖는 재조합 대장균균주 및 이를 이용한 트립토판 제조방법
KR20100015815A (ko)	2007-03-23	2010-02-12	메타볼릭 익스플로러	1,2-프로판디올 및 아세톨의 제조를 위한 미생물 및 방법
WO2009101757A1 (ja) *	2008-02-14	2009-08-20	Panasonic Corporation	コンデンサマイクロホン及びｍｅｍｓデバイス
KR20090092373A (ko)	2008-02-27	2009-09-01	씨제이제일제당 (주)	피루베이트 옥시다아제가 불활성화된 ｌ-트립토판 생산미생물 및 이를 이용한 ｌ-트립토판 생산 방법
EP2803720B1 (en) *	2012-01-10	2022-03-09	Cj Cheiljedang Corporation	Microorganisms of escherichia coli having enhanced l-tryptophan production and method for producing l-tryptophan using same

2014
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- 2015-06-23 US US15/321,717 patent/US10815510B2/en active Active
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ES2918459T3 (es)	2022-07-15
KR20160000082A (ko)	2016-01-04
DK3159401T3 (da)	2022-07-04
US20170137854A1 (en)	2017-05-18
WO2015199406A1 (ko)	2015-12-30
RU2016152662A (ru)	2018-07-23
BR112016030467B1 (pt)	2023-11-14
EP3159401A1 (en)	2017-04-26
RU2678139C2 (ru)	2019-01-23
JP2019013256A (ja)	2019-01-31
KR101835173B1 (ko)	2018-03-07
CN106574239A (zh)	2017-04-19
CN106574239B (zh)	2024-05-14
JP2017518759A (ja)	2017-07-13
US10815510B2 (en)	2020-10-27
EP3159401B1 (en)	2022-05-18
BR112016030467A2 (pt)	2018-01-16
EP3159401A4 (en)	2018-01-10
MY172369A (en)	2019-11-21
RU2016152662A3 (es)	2018-07-23

Publication	Publication Date	Title
KR101261147B1 (ko)	2013-05-06	Ｌ-아미노산의 생산능이 향상된 미생물 및 이를 이용하여 ｌ-아미노산을 생산하는 방법
JP6870041B2 (ja)	2021-05-12	Ｌ−トリプトファン生産能を有するエシェリキア属微生物及びそれを用いたｌ−トリプトファンの製造方法
EP3272860B1 (en)	2023-08-09	Pyruvate dehydrogenase mutant, microorganism comprising mutant, and method for producing l-amino acid by using microorganism
JP2019013256A (ja)	2019-01-31	Ｌ−トリプトファン生産能を有するエシェリキア属微生物及びこれを用いたｌ−トリプトファンの製造方法
JP6375391B2 (ja)	2018-08-15	Ｏ−アセチル−ホモセリンを生産する微生物及びこれを用いてｏ−アセチル−ホモセリンを生産する方法
JP7383046B2 (ja)	2023-11-17	Ｌ－アミノ酸を生産する微生物及びそれを用いたｌ－アミノ酸の生産方法
JP6596105B2 (ja)	2019-10-23	Ｌ−トリプトファンを産生するエシェリキア属の微生物及びそれを用いたｌ−トリプトファンの製造方法
JP2018161154A (ja)	2018-10-18	Ｌ−スレオニン産生能を有する組換えエシェリキア属微生物およびこれを用いたｌ−スレオニンの産生方法
JP6177995B2 (ja)	2017-08-09	Ｌ−トリプトファン産生能を有する微生物およびこれを用いてｌ−トリプトファンを産生する方法
EP3130663B1 (en)	2020-11-18	Microorganism having l-lysine producing ability and l-lysine producing method using same

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