JP6364842B2 - Cancer cell capturing device, treatment liquid kit, and method for producing treatment liquid kit - Google Patents

Description

  The present invention relates to a cancer cell capturing apparatus for capturing cancer cells that have entered the blood with a filter for use in testing for metastatic cancer or anticancer evaluation, and a treatment liquid kit and a treatment liquid kit used therefor It relates to a manufacturing method.

  Cancers, also called “malignant tumors”, have seriously hindered the life support of living organisms when they progress, and various treatment methods have been studied. There is the presence or absence of cancer metastasis as a guideline when deciding the treatment policy for cancer patients. Metastasis is caused by cancer cells invading blood vessels, riding on blood, spreading throughout the body, and cancer cells moving to other organs. Therefore, the measurement of the presence and amount of cancer cells in the blood is important information for predicting cancer metastasis.

As a conventional technique for capturing cancer cells in blood that causes metastasis, for example, as shown in Patent Document 1, a method of capturing cancer cells in blood with a filter is known. Patent Document 1 discloses a method for manufacturing a filter using a lithography technique, the shape of a cell unit that houses the filter, and the structure of a flow path for flowing blood and processing liquid. According to the method described in Patent Document 1, cells that do not pass through the filter are collected on the filter by flowing blood containing cancer cells through the cell unit containing the filter. The cells present on the filter are identified as cancer cells by staining.
In Patent Document 2, a plurality of reagent containers are directly connected to each other by a integrally formed connecting device for the purpose of finding a reagent handling means that allows flexible handling even in various reagent charges. A modular reagent cartridge is disclosed.

US Patent Publication No. 2011/0053152 Japanese National Patent Publication No. 11-515106

  To evaluate the cancer cells captured by the filter, the white blood cells that have the same size as the cancer cells and remain on the filter together with the cancer cells are stained with the cancer cells and observed under a microscope. To distinguish between cancer cells and white blood cells. In the process of capturing and staining cancer cells with a filter, various types of processing are performed using a plurality of types of processing solutions. The following process is mentioned as an example of the process using several types of process liquid. That is, a first cleaning process for first cleaning the entire flow path with a cleaning liquid, followed by a capturing process for capturing the cancer cells by passing the test liquid through the filter, and a second cleaning process for subsequently cleaning the filter with the cleaning liquid Subsequently, an immobilization treatment that protects cancer cells from spoilage using a fixing solution, followed by a third washing treatment for washing out the fixing solution, followed by penetration of the staining solution into the cancer cells captured on the filter A permeation treatment that dissolves the cell membrane of cancer cells using a permeation solution, followed by a fourth washing treatment for washing out the permeation solution, followed by staining that stains cancer cells captured on the filter using a staining solution. This is a fifth cleaning process in which the process and the staining solution are washed away. After these processes are performed, observation with a microscope is performed.

  Thus, in order to observe a cancer cell, there are many operations that must be performed so far, which is complicated. In addition, when a commercially available processing solution is used when performing a plurality of treatments as described above, the effect may vary depending on the manufacturer and the combination with other processing solutions, even if the same type of processing solution is used. . For this reason, sufficient reproducibility may not be obtained.

  The present invention has been made in view of the above, and a cancer cell trapping device improved in work efficiency for capturing cancer cells and improved in reproducibility, a treatment liquid kit used therefor, and a production of the treatment liquid kit It aims to provide a method.

  In order to achieve the above object, the inventors of the present invention have made reproducibility to make a processing solution kit in which processing solutions whose reproducibility has been sufficiently confirmed are enclosed in a container and integrally connected as a result of intensive studies. It was found to be very useful for improving security and workability. Furthermore, in view of the fact that the staining solution is currently expensive and difficult to store at room temperature for a long period of time, these problems can be solved by making the amount almost useable in several tests related to the capture of cancer cells. I found that I can clear. That is, the treatment liquid kit according to the present invention is used in a cancer cell capturing device that captures cancer cells in a test solution with a filter, and is used for a series of processes for capturing and observing cancer cells. A treatment liquid kit comprising a plurality of treatment liquid containers each enclosing a plurality of kinds of treatment liquids, and a connecting means for integrally connecting the plurality of treatment liquid containers, wherein the treatment liquid stains cancer cells The amount of the processing liquid enclosed in the plurality of processing liquid containers is set so that the staining liquid disappears at the same timing as the other processing liquid or earlier than the other processing liquid. It is characterized by that.

  Further, in the above processing solution kit, the amount of staining solution enclosed in the processing solution container is set based on m times (m is a natural number) the amount of staining solution required for performing a series of processing once. The amount of other processing liquids that are different from the staining liquid for each of the other processing liquid containers that are different from the processing liquid container in which the staining liquid is enclosed is necessary when performing a series of processes once. It can be set as the aspect set so that it may become m times or more of each process liquid.

  Further, the cancer cell capturing device according to the present invention has a test liquid container containing a test liquid and a through-hole that captures cancer cells in the test liquid by passing the test liquid. A plurality of treatment liquid containers each containing a plurality of kinds of treatment liquids used in a series of treatments for capturing and observing cancer cells on the filter, a test liquid container, and a plurality of treatment liquid containers Each of the treatment liquid containers and a flow path that connects one side of the main surface of the filter, wherein the plurality of treatment liquid containers are integrally connected by a connecting means. The treatment liquid contains a staining liquid that stains cancer cells, and the amount of treatment liquid enclosed in a plurality of treatment liquid containers is equal to the timing at which the staining liquid is the same as other treatment liquids or It is set so that it disappears earlier than other processing liquids. To.

    Further, in the above-described cancer cell capturing apparatus, the amount of staining liquid enclosed in the processing liquid container is set based on m times (m is a natural number) the amount of staining liquid necessary for performing a series of processes once. The amount of the other processing liquid different from the staining liquid for each of the other processing liquid containers different from the processing liquid container in which the staining liquid is enclosed among the plurality of processing liquid containers is determined when performing a series of processes once. It is set so that it may become m times or more of each process liquid required for this.

  According to the treatment liquid kit and the cancer cell capture device, the treatment liquid container in which a plurality of treatment liquids used for a series of treatments for capturing and observing cancer cells on a filter is enclosed by the connecting means. The treatment liquid includes a staining liquid that stains cancer cells, and the amount of the processing liquid enclosed in the plurality of processing liquid containers is the same as the timing at which the staining liquid is the same as other processing liquids or By setting each so as to disappear earlier than the other processing liquids, the timing of replacement of the processing liquid can be minimized, so that workability is improved. It is preferable to use all the processing liquids sealed in a plurality of processing liquid containers at the same timing as much as possible. However, since the processing liquids other than the staining liquid are relatively inexpensive compared to the staining liquid, the staining liquid is eliminated. Sometimes some may remain. In this case, workability can be improved by replacing the remaining processing solution with a new processing solution kit without using it.

  In the treatment liquid kit and the cancer cell capturing apparatus, the amount of the staining liquid enclosed in the treatment liquid container is m times (m is a natural number) the amount of the staining liquid necessary for performing a series of treatments once. The amount of the other treatment liquid enclosed in the treatment liquid container is set to be m times or more of each treatment liquid required when performing a series of treatments once. Compared with the case of preparing individually, each processing solution including the staining solution can be exchanged at once, so that the work efficiency is improved. Furthermore, reproducibility in capturing and observing cancer cells can be improved by arranging treatment liquids used for a series of treatments as a treatment liquid kit and repeatedly using them for capturing and observing cancer cells. In addition, by preparing a treatment solution used for capturing and observing cancer cells as a kit, reproducibility can be improved by minimizing the lot difference between treatment solutions.

  Here, “a series of treatments” refers to the period from setting the treatment solution kit to the cancer cell capture device to observing cancer cells captured on the filter by passing one test solution through the filter. This is a process for one routine performed in the meantime. Specifically, a first cleaning process in which the entire flow path is first cleaned with a cleaning liquid, a capturing process in which a test solution is passed through a filter to capture cancer cells, and a filter is subsequently cleaned with a cleaning liquid. Washing treatment, followed by immobilization treatment that protects cancer cells from spoilage using a fixing solution, followed by a third washing treatment for washing out the fixing solution, followed by staining inside the cancer cells captured on the filter Permeation treatment that dissolves the cell membrane of cancer cells using the permeate so that the liquid can permeate, followed by a fourth washing treatment that rinses the permeate, followed by the cancer cells captured on the filter using the staining solution The dyeing process for dyeing and the fifth washing process for washing away the dyeing solution are included.

  “Integral” means “to the extent that it can be handled as one”. In addition, “set based on m times (m is a natural number) of the amount of staining solution necessary for performing a series of treatments” means that the amount of staining solution is a single amount of staining solution × m times + α. It is to be. Here, as factors for determining α, (A) the amount of the processing liquid remaining in the processing liquid container of the staining liquid after finishing m times of processing, and (B) the processing liquid after finishing processing of m times The amount of processing liquid remaining in the flow path from the container to the selection valve can be mentioned. In addition, “setting to be m times or more of each processing solution necessary for performing a series of processing” means that the amount of each processing solution is set to one processing liquid amount × m times + β. is there. Here, as factors determining β, in addition to the amount of processing liquid according to (A) and (B) above, (C) the amount of cleaning liquid used for additional cleaning work caused by unsteady work, etc. Can be mentioned. Specifically, the amount of the treatment liquid based on (A) is about 5% by volume of the enclosed amount. Further, the amount of the processing liquid based on (B) is a flow path volume from the processing liquid container to the selection valve. During processing, the processing liquid is retained in the flow path, thereby preventing bubbles from being mixed due to entrainment. In addition, the amount of the cleaning liquid based on (C) can be appropriately estimated and filled, and for example, the amount of the cleaning liquid can be about 5 treatments.

That is, the amount of staining liquid enclosed can be expressed by the following equation (1).
Enclosed amount of staining solution = (amount of staining solution for one time) × m + amount of staining solution based on (A) + amount of staining solution based on (B) Formula (1)

In addition, the amount of other treatment liquid enclosed can be expressed by the following equations (2) and (3).
Enclosed amount of fixative or permeate = (Amount of treatment liquid for one batch) × m + Amount of treatment liquid based on (A) + Amount of treatment liquid based on (B) (2)
Enclosed amount of cleaning liquid = (processing liquid amount for one time) × m + processing liquid volume based on (A) + processing liquid volume based on (B) + processing liquid volume based on (C) Formula (3)

  Here, the staining solution is obtained by labeling an antibody that reacts with an antigen specific to cancer cells or leukocytes with a fluorescent dye, and cannot be stored at room temperature for a long time. Therefore, the amount of staining solution enclosed in the processing solution container is set based on m times the amount of staining solution required when performing a series of processing once (m is a natural number), By setting the amount of other processing liquids to be m times or more of each processing liquid required when performing a series of processing once, the timing of replacement of each processing liquid container can be easily adjusted. By exchanging the treatment solution as a treatment solution kit, the treatment solution containers can be exchanged at a time rather than individually, so that the working efficiency can be remarkably improved.

  Here, the bottom of the inner wall of the processing liquid container has a tapered shape in which the cross-sectional area decreases from the top to the bottom, and when the amount of processing liquid inside the processing liquid container decreases, Since it accumulates, the enclosed processing liquid can be used without leaving more, and it is more preferable.

  Further, it is more preferable that the cross section of the inner wall of the processing liquid container is circular, because the possibility that the processing liquid inside the processing liquid container remains attached to the inner wall can be reduced.

  Further, it is more preferable that the connecting means is a lid member that integrally closes the openings of the plurality of processing liquid containers, since the effect of improving workability is excellent.

  In addition, when the connecting means is constituted by a connecting member that can be detached from each processing solution container, only the processing solution container containing the staining solution that needs to be refrigerated is separated from other processing solution containers before use. It is more preferable because it can be stored in a refrigerator and is excellent in workability.

  Further, it is more preferable to further include an identifier for identifying the own kit, because it becomes easy to manage the time and the remaining amount after opening the treatment liquid kit.

  The method for producing a treatment liquid kit according to the present invention is a method for producing a treatment liquid kit used in a cancer cell capture device for capturing cancer cells in a test solution with a filter, and captures cancer cells. Preparing a plurality of treatment liquid containers for a plurality of types of treatment liquids used in a series of treatments for observation, enclosing a treatment liquid in each of the plurality of treatment liquid containers, and a plurality of A step of integrally connecting the processing liquid containers by a connecting means, and the processing liquid contains a staining liquid that stains cancer cells and encloses the processing liquid sealed in a plurality of processing liquid containers The amount is set such that the staining liquid is set at the same timing as other processing liquids or earlier than the other processing liquids.

    Further, in the above-described method for manufacturing a treatment solution kit, the amount of staining solution enclosed in the treatment solution container is set based on m times (m is a natural number) the amount of staining solution required for performing a series of treatments once. Of the plurality of processing liquid containers, the amount of the other processing liquid different from the staining liquid for each of the other processing liquid containers different from the processing liquid container in which the staining liquid is enclosed is performed once as a series of processes. It is set so that it may become m times or more of each processing liquid required at the time.

  Since the treatment liquid kit obtained by such a manufacturing method can exchange the treatment liquids including the staining liquid in a lump compared with the case where the treatment liquids are individually prepared, the working efficiency is improved. Furthermore, reproducibility in capturing and observing cancer cells can be improved by arranging treatment liquids used for a series of treatments as a treatment liquid kit and repeatedly using them for capturing and observing cancer cells.

  Here, the connecting means is a lid member that integrally closes the openings of the plurality of treatment liquid containers, and the process liquid is sealed by integrally closing the openings of the plurality of treatment liquid containers with the lid member. , By simultaneously performing the step of integrally connecting a plurality of processing liquid containers, it is possible to perform the sealing of the processing liquid and the connection of the plurality of processing liquid containers at a time, and the number of processing liquid kits can be reduced with fewer steps. Manufacturing can be performed.

  ADVANTAGE OF THE INVENTION According to this invention, the cancer cell capture apparatus improved in the reproducibility with the improvement of the working efficiency which concerns on the capture | acquisition of a cancer cell, the processing liquid kit used for it, and the manufacturing method of a processing liquid kit are provided.

It is a figure explaining the composition of the cancer cell capture device concerning a 1st embodiment. FIG. 2A is a perspective view illustrating the configuration of the treatment liquid kit according to the first embodiment, and FIG. 2B is a cross-sectional view taken along the line IIB-IIB in FIG. It is sectional drawing explaining the process liquid kit which concerns on 2nd Embodiment. FIG. 4A is a perspective view illustrating the configuration of the treatment liquid kit according to the third embodiment, and FIG. 4B is a cross-sectional view taken along the line IVB-IVB in FIG. FIG. 5A is a perspective view illustrating the configuration of the processing liquid kit according to the fourth embodiment, and FIG. 5B is a top view of the processing liquid kit according to the fourth embodiment. It is sectional drawing explaining the processing liquid kit which concerns on 5th Embodiment. FIG. 7A is a perspective view illustrating the configuration of the processing liquid kit according to the sixth embodiment, and FIG. 7B is a top view of the processing liquid kit according to the sixth embodiment. FIG. 8A is a perspective view illustrating the configuration of the processing liquid kit according to the seventh embodiment, and FIG. 8B is a top view of the processing liquid kit according to the seventh embodiment. It is a figure explaining the modification of a process liquid kit.

  The preferred embodiments of the present invention will be described below in detail with reference to the accompanying drawings. However, the present invention is not limited to the following embodiments, and various modifications may be made within the scope of the invention. it can. In the description of the drawings, the same elements are denoted by the same reference numerals, and redundant description is omitted.

(First Embodiment-Cancer Cell Capture Device)
FIG. 1 is a diagram illustrating a configuration of a cancer cell capturing device according to the first embodiment. A cancer cell capturing device is a device that captures cancer cells contained in blood by filtering blood as a test solution using a filter. In addition, the cancer cells captured by the filter are stained using a treatment solution such as a staining solution, thereby identifying the cancer cells and counting the number of individual cancer cells.

  As shown in FIG. 1, the cancer cell capturing device 100 supplies a processing liquid (reagent) to the filter unit 2 in which a filter 1 for capturing cancer cells is provided, and the filter unit 2. A flow path 3 made of a soft tube and a flow path 4 made of a soft tube for supplying a test liquid (blood) to the filter unit 2 are provided. A processing liquid kit 6 including a plurality of processing liquid containers 5 is connected to the upstream side of the flow path 3. A plurality of processing liquid containers contain processing liquids (reagents) for performing processing such as staining. Examples of the processing liquid charged into the processing liquid container 5 include a staining liquid, a cleaning liquid, a permeating liquid, and a fixing liquid. Details of the treatment liquid will be described later. A flow path 7 made of a soft tube is inserted into each of the plurality of processing liquid containers 5 to form individual flow paths. These flow paths are connected to the selection valve 8, and the processing liquid container 5 containing the processing liquid connected to the flow path 3 is selected by rotating the selection valve 8.

  Connected to the flow path 4 connected to the filter unit 2 is a blood container 10 (test liquid container) in which blood suspected of containing cancer cells is contained as a test liquid. The filter unit 2 is configured to supply either one of the processing liquid and the blood instead of supplying the processing liquid and the blood at the same time. It is switched by valves 12 and 13 attached to 3 and 4 respectively. For example, when supplying blood to the filter unit 2, the valve 12 is closed and the valve 13 is opened. As the valves 12 and 13, pinch valves or the like for blocking the flow by pressurizing and deforming the soft tube can be used.

  Further, when any one of the processing liquid and the blood is supplied to the filter unit 2, the liquid is supplied by being sucked by the pump 14 provided downstream of the filter unit 2. The pump 14 has a structure capable of changing the flow rate of the liquid in the flow path by changing the rotation speed. As the pump 14, for example, a peristaltic pump (peristaltic pump) that sequentially moves a peristaltic point by pressurizing the soft tube can be used. By driving the pump 14, liquids such as processing liquid and blood flow in the direction toward the filter unit 2 through the flow path 3 or the flow path 4 and are supplied to the filter unit 2. The liquid that has passed through the filter 1 of the filter unit 2 is structured to flow into the waste liquid tank 16 through the flow path 15. With these structures, cancer cells in the blood are captured by the filter 1 provided on the flow path in the filter unit 2, and the cancer cells are stained with the staining solution.

  The filter unit 2 accommodates therein a filter 1 having a plurality of through holes 1A formed in the main surface in the thickness direction. The filter 1 is made of, for example, metal or polymer. On the upstream side of the filter 1 of the filter unit 2, a flow path 3 </ b> A connected to the flow path 3 formed of a soft tube and a flow path 4 </ b> A connected to the flow path 4 are formed. Further, on the downstream side of the filter 1 on the side opposite to the side where the flow paths 3A, 4A are formed, a flow path 15A for discharging the liquid that has passed through the through hole 1A of the filter 1 to the outside is provided. The through-hole 1A of the filter 1 is sized so that the cancer cells that are the capture target cannot pass through, and the cancer cells are captured on the filter 1 when blood passes through the through-hole 1A.

  Here, a part of blood cell components such as leukocytes that could not pass through the filter 1 other than the cancer cells remain on the filter 1, and these blood cell components and cancer cells are dyed separately by staining. Detect cells. In the washing and staining for detecting the captured cancer cells, after the blood filtering is completed, a staining solution, a washing solution, a permeating solution, and a fixing solution are supplied from the channel 3 of the tube connected to the channel 3A. Done in By this operation, the cancer cells are captured using the filter 1 and stained with the treatment liquid, and then the cancer cells on the filter 1 are identified and the number of cancer cells is measured.

(First embodiment-treatment liquid kit)
Next, the treatment liquid kit will be described with reference to FIG. 2A is a perspective view illustrating the configuration of the treatment liquid kit, and FIG. 2B is a cross-sectional view taken along the line IIB-IIB of the treatment liquid kit excluding the flow path 7 and the suction nozzle 71 in FIG. It is sectional drawing. In the processing liquid kit 6 described in the following embodiment, a cleaning liquid is sealed in the processing liquid container 5A, a fixing liquid is sealed in the processing liquid container 5B, and a permeating liquid is sealed in the processing liquid container 5C. A configuration in which the staining liquid is sealed in the processing liquid container 5D will be described.

  Here, the outline about the processing liquid used in the process which concerns on the identification of the cancer cell after the capture | acquisition operation | work of the cancer cell in blood, the quantity measurement of a cancer cell, etc. is demonstrated. The cleaning liquid is a processing liquid used for cleaning or removing the remaining amount of the chemical liquid used in the previous step. Examples of the washing liquid include a solution prepared by mixing bovine serum albumin and ethylenediaminetetraacetic acid in phosphate buffered saline to adjust the pH to about 7.4. The fixing solution is a treatment solution for performing a chemical treatment for protecting a biological sample from degradation due to self-degradation or spoilage. Examples of the fixing solution include a solution in which paraformaldehyde is mixed in 1 to 4% by mass in phosphate buffered saline. The permeation liquid is a treatment liquid for dissolving the cell membrane of cancer cells captured on the filter so that, for example, a dye and a staining liquid can permeate the polymer substance. Examples of the permeated liquid include a solution in which polyoxyethylene (10) octylphenyl ether is mixed with phosphate buffered saline. The staining liquid is a treatment liquid for labeling a fluorescent dye with respect to an antibody that reacts with a target antigen. Examples of the staining solution include 2 ′-(4-ethoxyphenyl) -5- (4-methyl-1-piperazinyl) -2,5′-bi-1H benzimidazole trihydrochloride aqueous solution, human leukocyte antigen and cytokeratin. And a solution obtained by diluting a mixed solution of (Cytokeratin) with phosphate buffered saline. These treatment liquids are used for treatment relating to capture and observation of cancer cells.

  Returning to FIG. 2, the treatment liquid containers 5 </ b> A to 5 </ b> D included in the treatment liquid kit 6 are each made of, for example, a resin material. Each of the processing liquid containers 5A to 5D has a circular cross section and includes a storage part 51 in which the processing liquid is stored. The processing liquid containers 5A to 5D include a flange part 52 that extends outward from the upper end at the opening of the storage part 51. And the upper surface side of each flange part 52 of process liquid container 5A-5D is adhere | attached on the lower surface side of the thin plate 55 which functions as a cover member. The thin plate 55 is preferably, for example, a resin film such as a silicone resin, a metal foil such as an aluminum foil, or a resin film such as a silicone resin or a polyolefin resin coated with aluminum. As the treatment liquid containers 5A to 5D, resins and glass are preferable, and polyolefin resins and the like are particularly preferable because they are excellent in chemical resistance and handleability. By providing such a configuration, the openings of the plurality of processing liquid containers 5A to 5D are integrally closed by the thin plate 55, and the processing liquid containers 5A to 5D are connected. That is, the thin plate 55 serves as a connection unit that integrally connects the treatment liquid containers 5A to 5D. Thereby, the plurality of processing liquid containers 5 </ b> A to 5 </ b> D are integrated as the processing liquid kit 6.

  The treatment liquid container 5D of the treatment liquid kit 6 includes a container in which a staining liquid for staining cancer cells is enclosed, and the amount of treatment liquid enclosed in the plurality of treatment liquid containers 5D is different from the staining liquid. Are set so as to disappear at the same timing as the other processing liquid or earlier than the other processing liquid. Here, “eliminates” means that the remaining amount in the processing liquid container 5D becomes less than the amount of liquid necessary for one process.

  As a more preferred embodiment, the treatment liquid container 5D of the treatment liquid kit 6 is based on m times (m is a natural number) of the amount necessary to perform a series of treatments related to capturing and observing cancer cells once. A set amount of staining solution is contained. The treatment liquid containers 5A to 5C contain an amount of treatment liquid that is set to be m times or more of the amount necessary to perform a series of treatments for capturing and observing cancer cells once. ing.

  The volume ratio of the processing liquid in one process is, for example, cleaning liquid (5A): fixing liquid (5B): permeating liquid (5C): staining liquid (5D) = 5: 1: 2: 1. The volume ratio of each treatment solution is determined based on, for example, the configuration of the cancer cell capturing device (filter size, flow path length, etc.) and the like.

  Further, “m” for specifying the number of times of a series of processing performed using one processing liquid kit 6 includes, for example, 10. This “m” can be appropriately set according to the standard time required for one series of treatments for capturing and observing cancer cells, the storage period of the staining solution, or the like.

  Table 1 exemplifies the amount of each treatment liquid enclosed when m = 10 and calculated based on the above equations (1) to (3). The volume of the flow path from the treatment liquid container to the selection valve was about 0.3 mL (flow path inner diameter φ1 mm, length 350 mm). Further, the amount of the processing liquid remaining in the processing liquid container of the staining liquid after 10 times of processing was 5% by volume of the enclosed amount.

Here, based on the case where the volume ratio of the treatment liquid in one treatment is set to wash liquid (5A): fixing liquid (5B): permeate liquid (5C): staining liquid (5D) = 5: 1: 2: 1. However, the concentration of the treatment liquid may be appropriately adjusted so that the amount of the fixing liquid, the permeation liquid, and the staining liquid is approximately the same. Further, regarding m = 10, the number of series of processes is based on the fact that the number of processes that can be performed per day is about 10 even if the processes are performed day and night. In the future, m can be increased if the number of times of processing increases as the technology advances.

  By adopting such an embodiment, it is possible to easily adjust the timing of replacement of each processing liquid container, and it is possible to replace the processing liquid kit at once instead of replacing it individually as a processing liquid kit. It is preferable in that it can be remarkably improved.

  Further, when the cross section is circular like the treatment liquid containers 5A to 5D of the treatment liquid kit 6, corners are not formed on the inner wall. Therefore, in the processing liquid containers 5A to 5D, it is possible to avoid the processing liquid from being collected at the corners of the inner wall.

  The treatment liquid kit 6 is manufactured by the following method. First, a plurality of processing liquid containers 5A to 5D for a plurality of types of processing liquids are prepared. Subsequently, the processing liquid is introduced into each of the plurality of processing liquid containers 5A to 5D. In the case of the processing liquid kit 6, the cleaning liquid is charged into the processing liquid container 5A, the fixing liquid is charged into the processing liquid container 5B, the permeated liquid is charged into the processing liquid container 5C, and the staining liquid is charged into the processing liquid container 5D. After that, the processing liquid containers 5A to 5D are closed by the thin plate 55 which is a lid member, so that the processing liquid is sealed in the processing liquid containers 5A to 5D. At the same time, these processing liquid containers 5 </ b> A to 5 </ b> D are integrally connected by the thin plate 55. Thereby, the treatment liquid kit 6 is formed. As described above, when the processing liquid containers 5A to 5D are closed by the thin plate 55 and the processing liquid containers 5A to 5D are connected at the same time, the processing liquid is sealed and the plurality of processing liquid containers are connected at a time. The processing solution kit can be manufactured with fewer steps.

  Then, when the treatment liquid kit 6 is used, the suction nozzle 71 connected to the flow path 7 is inserted through the thin plate 55 as shown in FIG. As a result, the treatment liquid containers 5 </ b> A to 5 </ b> D are opened, and the internal treatment liquid can be supplied to the filter unit 2 by the suction nozzle 71. In FIG. 2A, the flow path 7 and the suction nozzle 71 are illustrated for the purpose of explanation, but these are not included in the treatment liquid kit of the present invention.

  Moreover, when the series of processes related to the capture of cancer cells is completed m times as set forth above, the treatment liquid containers 5A to 5D can be exchanged all at once by exchanging the treatment liquid kit. Therefore, it is excellent in workability and preferable. In the case where it is necessary to perform a pretreatment such as a degassing treatment, it is preferable that the treatment liquid kit 6 is integrally connected so that the treatment liquid can be collectively degassed.

  Thus, in the treatment liquid kit 6 in the cancer cell capturing device 100 according to this embodiment, the treatment liquid containers 5A to 5D in which a plurality of treatment liquids used for a series of treatments for observing cancer cells are enclosed. Are integrally connected by a thin plate 55 which is a connecting means. Since the treatment liquid kit 6 has such a configuration, work such as pretreatment can be performed at a time as compared with a case where each treatment liquid is purchased and prepared individually, and work efficiency is improved. Furthermore, reproducibility in capturing and observing cancer cells can be improved by arranging the processing solution used for a series of processing as the processing solution kit 6 and using it for capturing and observing cancer cells.

  Hereinafter, the second to eighth embodiments will be described. Since all the treatment liquid kits according to the second to eighth embodiments can be used in the cancer cell capturing apparatus 100 shown in the first embodiment, only the configuration related to the treatment liquid kit will be described in the following embodiments. . Moreover, about the process liquid kit manufactured by the method similar to the process liquid kit 6 demonstrated in 1st Embodiment, the description which concerns on a manufacturing method is abbreviate | omitted.

(Second Embodiment)
FIG. 3 is a cross-sectional view illustrating a treatment liquid kit according to the second embodiment. The processing liquid kit 6A shown in FIG. 3 is obtained by integrally connecting four processing liquid containers 5I with thin plates 55. The point that the thin plate 55 functions as a connecting means is the same as the processing liquid kit 6 of the first embodiment. The difference between the processing liquid kit 6A according to the second embodiment and the processing liquid kit 6 of the first embodiment is the shape of the bottoms of the four processing liquid containers 5I.

  Specifically, the processing liquid container 5I has an annular first bottom portion 57B connected to the inner wall 57A, and a concave shape surrounded by the first bottom portion 57B and provided below the first bottom portion 57B. And a second bottom 57C. At this time, the tip of the suction nozzle 71 connected to the flow path 7 is inserted below the first bottom 57B. Further, of the processing liquid in the processing liquid container 5I, the processing liquid above the position where the tip 71A of the suction nozzle 71 reaches can be sucked, and the processing liquid L1 at a position lower than the tip 71A is not sucked. It remains in the processing liquid container 5I. This is preferable because air bubbles can be prevented from being mixed in the suction nozzle 71 by mistake. Here, when the amount of the processing liquid remaining in the processing liquid container after m times of processing is set to the volume of the concave second bottom portion 57C provided below the first bottom portion 57B, This is preferable because it is possible to more reliably prevent bubbles from being mixed.

  In the treatment liquid kit 6A, the bottom of the treatment liquid container 5I has a two-stage bottom part including a first bottom part 57B and a second bottom part 57C. As a result, the residual liquid of the processing liquid easily collects at the bottom, and when the tip 71A of the suction nozzle 71 is positioned below the first bottom 57B, the amount of the processing liquid L1 remaining at the bottom can be reduced. It is possible to reduce the waste of the processing liquid in the processing liquid container 5I and use it efficiently.

  In FIG. 3, the flow path 7 and the suction nozzle 71 are illustrated for explanation, but these are not included in the treatment liquid kit of the present invention.

(Third embodiment)
FIG. 4A is a perspective view illustrating the configuration of the treatment liquid kit according to the third embodiment, and FIG. 4B is a cross-sectional view taken along the line IVB-IVB in FIG. The processing liquid kit 6B shown in FIG. 4 is different from the processing liquid kit 6 of the first embodiment in that four processing liquid containers 5J are formed by one container member 58.

  In the processing liquid kit 6B, four concave portions 58A are formed in the container member 58, so that these concave portions 58A function as the processing liquid container 5J. Then, after the processing liquid is sealed in each of the processing liquid containers 5J, the upper surface part 58B extending in a substantially planar shape from the upper end of the recess 58A is bonded to the lower surface side of the thin plate 59, thereby functioning as the processing liquid container 5J. The opening of the recess 58A is closed. Such a container member 58 can be obtained, for example, by injection molding a resin material.

  In the processing liquid kit 6B, not only the thin plate 59 but also the upper surface portion 58B of the container member 58 functions as a connecting unit that integrally connects the plurality of processing liquid containers 5J. Thus, it is good also as a structure which changes the shape of the member by the side of the process liquid container 5J. In addition, it is preferable that the side wall of the processing liquid container 5J is shared with the adjacent processing liquid container so that the adjacent processing liquid containers 5J are connected to each other through the side wall because the processing liquid kit can be stored more compactly.

(Fourth embodiment)
FIG. 5A is a perspective view illustrating the configuration of the processing liquid kit according to the fourth embodiment, and FIG. 5B is a top view of the processing liquid kit according to the fourth embodiment. The processing liquid kit 6C shown in FIG. 5 relates to the first embodiment in that the connecting means for connecting the four processing liquid containers 5K is a frame member 76 provided with holes for attaching the processing liquid containers 5K. Different from the treatment liquid kit 6. The processing liquid kit 6 </ b> C according to the present embodiment has a configuration in which the processing liquid containers are connected to each other by a member different from the lid member that covers the opening of the processing liquid container. Note that the upper openings of the four processing liquid containers 5K are respectively closed by lid members or the like, but the illustration is omitted.

  The frame member 76 has a flat plate shape, and four holes 77 for attaching the treatment liquid container 5K are formed in the thickness direction thereof. Then, the four processing liquid containers 5K are integrally connected by inserting the processing liquid containers 5K into these holes 77. In addition, a non-slip member such as an elastic member or a fixing member such as an adhesive may be attached to the inside of the hole 77 so that the processing liquid container 5K does not move in the thickness direction of the frame member 76.

  Said process liquid kit 6C is manufactured, for example with the following method. First, a plurality of processing liquid containers 5K for a plurality of types of processing liquids are prepared. Subsequently, the processing liquid is charged into each of these four processing liquid containers 5K. Thereafter, the processing liquid is sealed in the processing liquid container 5K by closing each of the four processing liquid containers 5K with a lid member (not shown). Thereafter, these processing liquid containers 5K are attached to the frame member 76 so as to be integrally connected by the frame member 76. Thereby, the treatment liquid kit 6C is formed. In addition, it is good also as a structure which connects the four process liquid containers 5K previously by attaching the frame member 76, and encloses a process liquid in each process liquid container 5K after that.

(Fifth embodiment)
FIG. 6 is a cross-sectional view illustrating a treatment liquid kit according to the fifth embodiment. The processing liquid kit 6D shown in FIG. 6 is obtained by integrally connecting four processing liquid containers 5P with thin plates 55. The difference between the processing liquid kit 6D according to the fifth embodiment and the processing liquid kit 6 according to the first embodiment is the shape of the bottom of the four processing liquid containers 5P.

  Specifically, the bottom 57D of the processing liquid container 5P has a tapered shape in which the cross-sectional area decreases from the top to the bottom. At this time, the suction nozzle 71 connected to the flow path 7 is inserted below the peripheral edge 57F connected to the side wall 57E in the bottom 57D having a tapered shape. Here, of the processing liquid in the processing liquid container 5P, the processing liquid above the position where the tip 71A of the suction nozzle 71 reaches can be sucked, and the processing liquid L2 at a position lower than the tip 71A is not sucked. In the processing liquid container 5P. This is preferable because air bubbles can be prevented from being mixed in the suction nozzle 71 by mistake.

  In this way, in the processing liquid kit 6D, the bottom 57D of the processing liquid container 5P is tapered so that the cross-sectional area decreases from the top to the bottom, so that the residual liquid of the processing liquid easily collects at the bottom. When the tip 71A of the suction nozzle 71 is positioned below the peripheral edge 57F of the bottom 57D, the amount of the processing liquid L2 remaining on the bottom can be reduced. Therefore, in the treatment liquid kit 6D, waste of the treatment liquid in the treatment liquid container 5P can be reduced and used efficiently. When estimating the volume of the processing liquid in the processing liquid container 5P, the amount of the processing liquid L2 remaining at the bottom may be estimated to be about 5% by volume of the amount of each processing liquid enclosed. In FIG. 6, the flow path 7 and the suction nozzle 71 are illustrated for explanation, but these are not included in the treatment liquid kit of the present invention.

(Sixth embodiment)
FIG. 7A is a perspective view illustrating the configuration of the processing liquid kit according to the sixth embodiment, and FIG. 7B is a top view of the processing liquid kit according to the sixth embodiment. In the processing liquid kit 6E shown in FIG. 7, the connecting means for connecting the four processing liquid containers 5L to 5O is detachable individually for each connecting means, and is provided not on the top surface of the container but on the outer wall of the container. Is different from the treatment liquid kit 6 according to the first embodiment. Although the upper openings of the processing liquid containers 5L to 5O are respectively closed by lid members or the like, they are not shown.

  In the treatment liquid kit 6E, each of the four treatment liquid containers 5L to 5O has a bottomed cylindrical shape, but may have a tapered shape in which the cross-sectional area decreases from the bottom toward the bottom. Or it is good also as a structure which has a multi-stage bottom part like the process liquid container 5I of FIG. A connecting member 81 is attached to the outer wall of the processing liquid container 5L, a connecting member 82 is attached to the outer wall of the processing liquid container 5M, and a connecting member 83 is attached to the outer wall of the processing liquid container 5N. A connecting member 84 is attached to the outer wall of 5O. Each of the connecting members 81 to 84 includes a connecting portion with the processing liquid containers 5L to 5O and a concave and convex fitting portion for fitting with each other's connecting member. The processing portion kit 6E can be assembled by fitting the joint portions together. 7A and 7B, the connecting members 81 to 84 are indicated by alternate long and short dash lines in order to make the configuration of the treatment liquid kit 6E easier to understand.

  Each connection part of the connection members 81-84 can hold | grip a process liquid container so that it may each cover 1/4 or more of the outer periphery of each process liquid container. The processing liquid containers 5L to 5O are provided with flange portions 52L to 52O extending outward from the upper end portions at the openings of the respective containers, and the connecting members 81 to 84 can be locked by the flange portions 52L to 52O. The processing liquid kit 6E can detach the processing liquid containers 5L to 5O individually as many times as possible, and can detach the connecting members 81 to 84 as many times as possible. When the kit 6E is assembled, the treatment liquid kit can be handled as a single unit.

  The concave fitting portion 81B of the connecting member 81 and the convex fitting portion 82A of the connecting member 82 are fitted together to form a first connecting portion, and similarly, the concave fitting portion 82B of the connecting member 82 is formed. And the convex fitting part 83A of the connecting member 83 are fitted together to form a second connecting part, and similarly, the concave fitting part 83B of the connecting member 83 and the convex fitting part of the connecting member 84 84A is fitted to each other to form a third connecting portion, and similarly, the concave fitting portion 84B of the connecting member 84 and the convex fitting portion 81A of the connecting member 81 are fitted to each other to form a fourth. The processing liquid kit 6E can be assembled in a state in which the connecting portion is formed.

  The treatment liquid kit 6E is produced by the following method, for example. First, a plurality of processing liquid containers 5L to 5O for a plurality of types of processing liquids are prepared. It is set as the structure by which the connection members 81-84 are attached to the some process liquid containers 5L-5O, respectively. Subsequently, the processing liquid is introduced into each of the four processing liquid containers 5L to 5O. Thereafter, the processing liquid is sealed in the processing liquid containers 5L to 5O by closing each of the four processing liquid containers 5L to 5O with a lid member (not shown). Then, the process liquid containers 5L-5O are integrally connected by the connection members 81-84 by connecting the connection members 81-84, respectively. Thereby, the treatment liquid kit 6E is formed. In addition, it is good also as a structure which attaches the connection members 81-84 with respect to the four process liquid containers 5L-5O after enclosing a process liquid.

  Further, the processing liquid containers 5L to 5O are attached to the connecting members 81 to 84 in advance, and after connecting the connecting members 81 to 84, the processing liquid is charged and sealed with a lid member (not shown), and the processing liquid kit 6E is installed. It may be formed.

  Thus, the connection means for integrally connecting the plurality of treatment liquid containers 5L to 5O can be a member different from the lid member. Moreover, as shown in this embodiment, the structure which functions as a connection means by combining the some connection members 81-84 may be sufficient. By connecting the processing liquid container with such a connecting member, for example, the processing liquid container in which the staining liquid that cannot be stored at room temperature for a long time is enclosed is separated from other processing liquid containers and stored refrigerated until just before use. Is possible and preferred. Moreover, even if there is a defect such as a filling failure in any of the treatment liquid containers, it is possible to make a non-defective treatment liquid kit by replacing only the defective treatment liquid container, and the entire treatment liquid kit is regarded as defective. It is preferable because it does not need to be handled.

(Seventh embodiment)
FIG. 8A is a perspective view illustrating the configuration of the processing liquid kit according to the seventh embodiment, and FIG. 8B is a top view of the processing liquid kit according to the seventh embodiment. The processing liquid kit 6F shown in FIG. 8 is the sixth embodiment in that the connecting means for connecting the four processing liquid containers 5L to 5O is connected so that the processing liquid containers are arranged in one row. This is different from the processing solution kit 6E according to FIG. Although the upper openings of the processing liquid containers 5L to 5O are respectively closed by lid members or the like, they are not shown.

  In the treatment liquid kit 6F, each of the four treatment liquid containers 5L to 5O has a bottomed cylindrical shape, but may have a tapered shape in which the cross-sectional area decreases from the bottom toward the bottom. Or it is good also as a structure which has a multi-stage bottom part like the process liquid container 5I of FIG. A connecting member 85 is attached to the outer wall of the processing liquid container 5L, a connecting member 86 is attached to the outer wall of the processing liquid container 5M, and a connecting member 87 is attached to the outer wall of the processing liquid container 5N. A connecting member 88 is attached to the outer wall of 5O. Each of the connecting members 85 to 88 includes a connecting portion with the processing liquid containers 5L to 5O and a concave and convex fitting portion for fitting with each other's connecting member. The treatment part kit 6F can be assembled by fitting the joints together. The treatment liquid kit 6F can detach the treatment liquid containers 5L to 5O individually as many times as possible, and can detach the connecting members 85 to 88 as many times as possible. When the kit 6F is assembled, the treatment liquid kit can be handled as a single unit.

  The convex fitting portion 85A of the connecting member 85 and the concave fitting portion 86B of the connecting member 86 are fitted together to form a first connecting portion, and similarly, the convex fitting portion 86A of the connecting member 86. And the concave fitting portion 87B of the connecting member 87 are fitted together to form a second connecting portion, and similarly, the convex fitting portion 87A of the connecting member 87 and the concave fitting portion of the connecting member 88 The treatment liquid kit 6F can be assembled in a state where the 88B and the third connection portion are formed by fitting with each other. Here, the concave fitting portion 85B of the connecting member 85 and the convex fitting portion 88A of the connecting member 88 are fitting portions for cases where further connection is required.

  The treatment liquid kit 6F is produced by the following method, for example. First, a plurality of processing liquid containers 5L to 5O for a plurality of types of processing liquids are prepared. It is set as the structure by which the connection members 85-88 are attached to the some process liquid containers 5L-5O, respectively. Subsequently, the processing liquid is introduced into each of the four processing liquid containers 5L to 5O. Thereafter, the processing liquid is sealed in the processing liquid containers 5L to 5O by closing each of the four processing liquid containers 5L to 5O with a lid member (not shown). Then, the process liquid containers 5L-5O are integrally connected by the connection members 85-88 by connecting the connection members 85-88, respectively. Thereby, the treatment liquid kit 6F is formed. In addition, it is good also as a structure which attaches the connection members 85-88 with respect to the four process liquid containers 5L-5O after enclosing a process liquid.

  Further, the processing liquid containers 5L to 5O are attached to the connecting members 85 to 88 in advance, and after connecting the connecting members 85 to 88, the processing liquid is charged and sealed with a lid member (not shown) to seal the processing liquid kit 6F. It may be formed.

  Thus, the connection means for integrally connecting the plurality of treatment liquid containers 5L to 5O can be a member different from the lid member. Moreover, as shown in this embodiment, the structure which functions as a connection means by combining the some connection members 85-88 may be sufficient. By connecting the processing liquid container with such a connecting member, for example, the processing liquid container in which the staining liquid that cannot be stored at room temperature for a long time is enclosed is separated from other processing liquid containers and stored refrigerated until just before use. Is possible and preferred. Moreover, even if there is a defect such as a filling failure in any of the treatment liquid containers, it is possible to make a non-defective treatment liquid kit by replacing only the defective treatment liquid container, and the entire treatment liquid kit is regarded as defective. It is preferable because it does not need to be handled.

(Modification)
Although the embodiments of the treatment liquid kit and the cancer cell capturing device according to the present invention have been described above, various modifications can be made to the treatment liquid kit and the cancer cell capturing device according to the present invention.

  For example, as shown in FIG. 9, it is good also as a structure provided with the identifier 98 for distinguishing each kit with respect to the process liquid kit 6, and specifying an own kit. In FIG. 9, a configuration including a barcode as the identifier 98 is shown. In this manner, the treatment liquid kit 6 can be specified by assigning the identifier 98 to each kit. As a result of such a configuration, for example, the expiration date of the treatment liquid kit 6 can be managed, and further, for example, the type and amount of the treatment liquid in the treatment liquid kit 6 can be managed. Further, the apparatus for reading the identifier 98 of the treatment liquid kit 6 may be configured to be included in the cancer cell capturing apparatus, and the process management may be performed by reading the identifier 98.

  Moreover, in the said embodiment, although the structure using four types of process liquids was demonstrated in the series of processes which concern on capture | acquisition of a cancer cell, the number of process liquids is suitably set according to the process which concerns on capture | acquisition of a cancer cell. Be changed. In addition, the number of suction nozzles 71 and the number of flow paths 7 connected to the suction nozzles 71 in the cancer cell capturing device 100 are appropriately changed according to the number of steps or the number of treatment liquids. Further, for example, in the case of a configuration in which five types of processing liquids are used, the connecting member in FIG. 7 can adopt a structure that can be attached to and detached from five pieces, and can be used with appropriate modifications.

  Moreover, the structure of the filter unit 2 in which the filter 1 for capturing cancer cells is accommodated can be appropriately changed. Moreover, a connection means is not limited to the structure of the said embodiment.

  DESCRIPTION OF SYMBOLS 1 ... Filter, 2 ... Filter unit 3, 4, 7 ... Flow path, 5 (5A-5O) ... Processing liquid container, 6, 6A-6F ... Processing liquid kit, 8 ... Selection valve, 10 ... Test liquid container 55, 59 ... thin plate (lid member), 71 ... suction nozzle, 100 ... cancer cell trapping device.

Claims (14)

Used in a cancer cell capturing device that captures cancer cells in a test solution with a filter, each of which contains a plurality of types of treatment liquids used for a series of processes for capturing and observing the cancer cells. A plurality of processing liquid containers;
Connection means for integrally connecting the plurality of treatment liquid containers;
A treatment liquid kit comprising:
The treatment liquid contains a staining liquid for staining cancer cells,
The amount of the staining liquid enclosed in one of the plurality of processing liquid containers is based on m times (m is a natural number) the amount of staining liquid required for performing the series of processes once. The set amount of the other processing liquid different from the staining liquid for each of the other processing liquid containers different from the processing liquid container in which the staining liquid is sealed among the plurality of processing liquid containers is the series of processes. Is set to be m times or more of each processing solution required when performing once,
The m is a treatment liquid kit that is set according to a standard time required for one series of treatments for capturing and observing cancer cells or a storage period of the staining liquid. The processing liquid kit according to claim 1, wherein the bottom portion of the inner wall of the processing liquid container has a tapered shape in which a cross-sectional area decreases from the upper side to the lower side. The processing liquid kit according to claim 1 or 2 , wherein a cross section of the inner wall of the processing liquid container is circular. It said connecting means, the processing solution kit according to any one of claims 1 to 3 openings of the plurality of processing liquid container is a lid member for closing integrally.   The processing liquid kit according to any one of claims 1 to 4, wherein the connecting means includes a connecting member capable of individually attaching and detaching each processing liquid container. The treatment liquid kit according to any one of claims 1 to 5 , further comprising an identifier for identifying the self-kit. A test solution container containing the test solution;
A filter provided with a through-hole on the main surface for capturing cancer cells in the test liquid by passing the test liquid;
A plurality of treatment liquid containers each enclosing a plurality of kinds of treatment liquids used in a series of processes for capturing and observing the cancer cells on the filter;
A flow path connecting each of the test liquid container and the plurality of processing liquid containers and one side of the main surface of the filter;
A cancer cell capturing device comprising:
The plurality of processing liquid containers constitute a processing liquid kit integrally connected by a connecting means,
The treatment liquid contains a staining liquid for staining cancer cells,
The amount of the staining liquid enclosed in one of the plurality of processing liquid containers is based on m times (m is a natural number) the amount of staining liquid required for performing the series of processes once. The set amount of the other processing liquid different from the staining liquid for each of the other processing liquid containers different from the processing liquid container in which the staining liquid is sealed among the plurality of processing liquid containers is the series of processes. Is set to be m times or more of each processing solution required when performing once,
The m is a cancer cell capturing device set in accordance with a standard time required for one series of processes related to capturing and observing cancer cells or a storage period of the staining solution . The cancer cell capturing apparatus according to claim 7 , wherein a bottom portion of the inner wall of the treatment liquid container has a tapered shape in which a cross-sectional area decreases from the upper side to the lower side. The cancer cell capturing device according to claim 7 or 8 , wherein the inner wall of the treatment liquid container has a circular cross section. The cancer cell capturing apparatus according to any one of claims 7 to 9 , wherein the connecting means is a lid member that integrally closes the openings of the plurality of treatment liquid containers. The cancer cell capturing apparatus according to any one of claims 7 to 10 , wherein the connection means is configured by a connection member that can detach and attach each processing solution container individually. The cancer cell capturing device according to any one of claims 7 to 11 , further comprising an identifier for identifying the own kit in the treatment liquid kit. A method for producing a treatment liquid kit for use in a cancer cell capturing device for capturing cancer cells in a test liquid with a filter,
Preparing a plurality of treatment liquid containers for a plurality of kinds of treatment liquids used in a series of treatments for capturing and observing the cancer cells;
Enclosing a treatment liquid in each of the plurality of treatment liquid containers;
Connecting the plurality of processing liquid containers integrally by a connecting means;
Have
The treatment liquid contains a staining liquid for staining cancer cells,
The amount of the staining liquid enclosed in one of the plurality of processing liquid containers is based on m times (m is a natural number) the amount of staining liquid required for performing the series of processes once. The set amount of the other processing liquid different from the staining liquid for each of the other processing liquid containers different from the processing liquid container in which the staining liquid is sealed among the plurality of processing liquid containers is the series of processes. Is set to be m times or more of each processing solution required when performing once,
Said m is the manufacturing method of the process liquid kit set according to the standard time required for one series of processes concerning capture and observation of a cancer cell, or the shelf life of the said dyeing | staining liquid. The connecting means is a lid member that integrally closes the openings of the plurality of processing liquid containers,
The cover an opening of the plurality of processing liquid container by closing integrally by a member, a step of sealing the treatment solution, according to claim 13 for the step, at the same time integrally connecting the plurality of processing liquid container The manufacturing method of the processing liquid kit of description.

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