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JP6197646B2 - Meat modifier - Google Patents

Meat modifier Download PDF

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Publication number
JP6197646B2
JP6197646B2 JP2013525771A JP2013525771A JP6197646B2 JP 6197646 B2 JP6197646 B2 JP 6197646B2 JP 2013525771 A JP2013525771 A JP 2013525771A JP 2013525771 A JP2013525771 A JP 2013525771A JP 6197646 B2 JP6197646 B2 JP 6197646B2
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meat
calcium
rice starch
modifier
starch
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JPWO2013015401A1 (en
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広悌 薄衣
広悌 薄衣
朋美 小野
朋美 小野
小寺 智博
智博 小寺
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Ajinomoto Co Inc
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/60Comminuted or emulsified meat products, e.g. sausages; Reformed meat from comminuted meat product
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/422Addition of natural plant hydrocolloids, e.g. gums of cellulose derivatives or of microbial fermentation gums
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/40Meat products; Meat meal; Preparation or treatment thereof containing additives
    • A23L13/42Additives other than enzymes or microorganisms in meat products or meat meals
    • A23L13/428Addition of flavours, spices, colours, amino acids or their salts, peptides, vitamins, yeast extract or autolysate, nucleic acid or derivatives, organic acidifying agents or their salts or acidogens, sweeteners, e.g. sugars or sugar alcohols; Addition of alcohol-containing products

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Dispersion Chemistry (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Description

本発明は、食肉の肉質や食感等を改善し、ジューシーな食感を付与することを目的とする畜肉用食感改良剤及び食肉加工食品の製造法に関する。   The present invention relates to a texture improving agent for livestock meat and a method for producing a processed meat food for the purpose of improving the meat quality and texture of meat and imparting a juicy texture.

近年、鶏インフルエンザの発生などを経て、日本国内の鶏肉価格は増加傾向であり、ブラジルを代表とする安価な海外品の使用が普及している。海外品は、皮部の脂分は多いものの、肉質は硬く、ジューシー感はなく、国内鶏肉との品質差は大きい。また、牛肉についても、輸入牛肉は国産牛肉と比較して脂肪含量が少なく健康に適しているが、その一方で呈味的に劣り、また肉質も硬い。あるいは輸入牛肉ならびに国産牛肉の食感は部位によって大きく異なっており、結合組織が比較的多いスネ肉やスジ肉などの硬質肉は調理法が制限されている。以上から食肉の食感および品質改質技術の構築は重要な課題となっている。
一般に、食肉は屠殺後に内在性のカテプシンやカルパイン等のプロテアーゼにより骨格筋タンパク質が分解され、熟成・軟化することが知られている。そのため、肉質を改善する方法として、これまでプロテアーゼを用いた肉質の軟化法が検討・報告され(特開2007−319166号公報)、既に実用化されているものもある。これらプロテアーゼのうち最も一般的なものはパパイン、ブロメライン、アクチジニンであるが、これらはその基質特異性の低さと強力なタンパク質分解活性のために、食肉の結合組織や筋原組織を非特異的に分解してしまうので、処理された食肉の食感は肉本来の食感からかけ離れたものとなってしまう。また、例えば肉重量に対してわずか0.05重量%程度を肉に作用させただけでも過剰軟化を生じ、長時間放置すると更に分解が進行するため、肉質の改質の程度をコントロールするのが非常に難しい。この過剰軟化の問題は低温条件下で行っても制御が困難である。
それに対して基質特異性の高いプロテアーゼとしてエステラーゼやコラゲナーゼが肉軟化酵素として検討されている。しかし、これらの酵素を他のプロテアーゼの混入なく大量に精製・調製するのは困難である。また、これらの酵素で肉質を改善するには比較的多量の酵素を長時間反応させる必要がある。更に、実際、これらの酵素を肉に作用させても十分な効果が期待できない。
また、プロテアーゼ特有の重大な問題もある。食品用プロテアーゼの基質特異性は疎水性アミノ酸の前後を切断するものが殆どである。このようなプロテアーゼで分解して調製したペプチドは強烈な苦味を伴い、呈味の面で問題がある。
タンパク質加水分解酵素以外にも、各種食品素材を用いて、ジューシー及びソフト感があり美味しい食感・食味を有する等品質劣化防止対策の検討が為されてきた。例えば(1)アルカリ製剤(リン酸塩類、炭酸塩類)などの単独もしくは組み合わせで改質する方法(特開平4−36167号、特開平11−200823号、特許第2568946号、特開2000−60492)、(2)糖質により改質する方法(特許第3804571号、特許3268543号)、(3)乳化剤を配合する方法(特開平8−276074)、(4)有機酸を活用する方法(特開2002−159281、特開2000−106846、特開2001−178416号)、(5)アミノ酸を活用する方法(特許第2880183号、特開平8−196252、特開昭59−175870)、(6)カルシウムを活用する方法(特開平10−14536、特開平4−341160)、(7)タンパク加水分解物を活用する方法(特開平10−14536、特開平4−341160、特開平2004−329165)あるいは(8)これらの組み合わせ法など、それぞれの特性を利用して効果を見出す方法が知られている。しかし、いずれも充分な食感及び食味の改質効果は得られていないのが現状である。(1)のアルカリ製剤を用いる方法は、ジューシー感の向上と歩留まり効果はあるものの、体内カルシウムがリン酸塩により排出する問題があり、リン酸塩フリーの食品を好む消費者は増加している。また肉組織が均一化し、繊維感が損なわれるという大きな課題がある。更に時間が経過すると食感が硬くなり、品質低下や異味(主に苦味、渋み)が発生する。(2)の方法については、保湿性の付与効果があるものの単独での顕著な肉質改質効果は期待できない。化学処理澱粉を用いた肉軟化剤も存在するが、溶解性の低さから筋肉中への浸透が困難であり、また効果自体も顕著とは言えない。(3)の乳化剤による方法は肉質の表面にコーテイングする為、調理すると柔らかさが乏しいという問題があった。様々な用途で多様な乳化剤が開発されているが、十分な効果発現に至っていない。(4)の方法は単独では酸味を有する課題があると共に、通常は肉を硬化する作用が報告されているため、アルカリ製剤との併用が必須である。(5)の方法はグリシンの活用の記載があるものの、肉改質効果に対する効果は非常に低い。(6)の方法は塩化カルシウムや乳酸カルシウムが一般的に使用されているが、肉本来の繊維感を維持しつつ、十分な保水性が得ることはできず、ジューシー感を付与された好ましい品質には至らない。(7)の方法は卵白加水分解物による保水性向上についての記載があるが、乳化剤と同様肉質の表面にコーテイングする為、調理すると柔らかさが乏しいという問題があった。様々な用途で多様な乳化剤が開発されているが、十分な効果発現に至っていない。(8)の例としては、アルカリ金属塩化物とアルカリ金属炭酸塩及び/又はアルカリ金属炭酸水素塩を含む食肉改質剤(特開平10−370923)がある。これの添加効果を確認すると、食感及び食味が時間とともに劣化し、出来上がり時の機能(食肉の食感と食味のバランス)が損なわれておりジューシー感と肉の柔らかさの低下が認められた。このように、多数の先行技術はあるものの、また、無処理に比べれば多少の効果は認められるものの十分に満足できるレベルには至っておらず、食味と食感の向上を有する改質効果の優れた素材もしくは効率的な改質方法が更に求められている。
In recent years, due to the outbreak of chicken flu, the price of chicken in Japan has been on the rise, and the use of inexpensive overseas products typified by Brazil has become widespread. Although overseas products have a high fat content in the skin, the meat quality is hard, there is no succulent feeling, and the quality difference from domestic chicken is large. As for beef, imported beef has less fat content and is suitable for health compared to domestic beef, but on the other hand, it is inferior in taste and hard. Alternatively, the texture of imported beef and domestic beef varies greatly depending on the region, and the cooking method is limited for hard meat such as shin meat and stripe meat with relatively many connective tissues. From the above, the texture of meat and the construction of quality improvement technology are important issues.
In general, it is known that after slaughtering meat, skeletal muscle proteins are degraded by endogenous proteases such as cathepsin and calpain, and matured and softened. Therefore, as a method for improving meat quality, a meat quality softening method using a protease has been studied and reported so far (Japanese Patent Laid-Open No. 2007-319166), and some have already been put into practical use. The most common of these proteases are papain, bromelain, and actinidine, but they non-specifically connect meat and myogenic tissues to meat due to their low substrate specificity and strong proteolytic activity. Since the food is decomposed, the texture of the processed meat is far from the original texture of the meat. In addition, for example, even if only about 0.05% by weight of meat is allowed to act on the meat, excessive softening occurs, and the decomposition proceeds further when left for a long time. very difficult. This excessive softening problem is difficult to control even when performed under low temperature conditions.
On the other hand, esterase and collagenase have been studied as meat softening enzymes as proteases with high substrate specificity. However, it is difficult to purify and prepare these enzymes in large quantities without mixing with other proteases. Moreover, in order to improve meat quality with these enzymes, it is necessary to react a relatively large amount of enzymes for a long time. Furthermore, in fact, sufficient effects cannot be expected even if these enzymes are allowed to act on meat.
There are also significant problems specific to proteases. Substrate specificity of food proteases is mostly those that cleave before and after hydrophobic amino acids. Peptides prepared by degradation with such proteases have a strong bitter taste and have a problem in taste.
In addition to protein hydrolases, various food materials have been used to examine measures for preventing deterioration of quality, such as having a succulent and soft feeling and having a delicious texture and taste. For example, (1) a method of modifying an alkali preparation (phosphates, carbonates) alone or in combination (Japanese Patent Laid-Open No. 4-36167, Japanese Patent Laid-Open No. 11-008243, Japanese Patent No. 2568946, Japanese Patent Laid-Open No. 2000-60492) , (2) a method of modifying with a sugar (Patent Nos. 3804571 and 3268543), (3) a method of blending an emulsifier (JP-A-8-276074), and (4) a method of utilizing an organic acid (JPA 2002-159281, JP-A-2000-106846, JP-A-2001-178416), (5) Method of utilizing amino acids (Japanese Patent No. 2880183, JP-A-8-196252, JP-A-59-175870), (6) Calcium (7) Utilizing protein hydrolyzate (JP-A-10-14536, JP-A-4-341160) Law (JP 10-14536, JP-A-4-341160, JP-A-2004-329165) such as or (8) combinations method, a method of finding an effect by utilizing the respective characteristics are known. However, at present, sufficient texture and taste-modifying effects are not obtained. Although the method of using the alkaline preparation (1) has an effect of improving the juiciness and yield, there is a problem that calcium in the body is excreted by phosphate, and consumers who prefer phosphate-free foods are increasing. . There is also a major problem that the meat structure becomes uniform and the fiber feeling is impaired. Furthermore, the texture becomes harder as time elapses, and quality deterioration and taste (mainly bitterness, astringency) occur. Although the method (2) has a moisturizing effect, it cannot be expected to have a significant meat quality improvement effect alone. There are also meat softeners using chemically-processed starch, but it is difficult to penetrate into muscle due to low solubility, and the effect itself is not remarkable. Since the method using the emulsifier of (3) coats the meaty surface, there is a problem that softness is poor when cooked. Various emulsifiers have been developed for various uses, but sufficient effects have not been achieved. The method (4) alone has a problem of having a sour taste, and usually has been reported to cure meat, so that it is essential to use it together with an alkaline preparation. Although the method (5) describes the use of glycine, the effect on the meat reforming effect is very low. In the method (6), calcium chloride or calcium lactate is generally used, but sufficient water retention cannot be obtained while maintaining the original fiber feeling of meat, and a desirable quality imparted with a juicy feeling. It does not lead to. Although the method of (7) has a description about the water retention improvement by an egg white hydrolyzate, since it coats on the surface of meat like an emulsifier, there existed a problem that a softness was scarce when it cooked. Various emulsifiers have been developed for various uses, but sufficient effects have not been achieved. As an example of (8), there is a meat modifier (Japanese Patent Laid-Open No. 10-370923) containing an alkali metal chloride and an alkali metal carbonate and / or an alkali metal bicarbonate. When the effect of addition was confirmed, the texture and taste deteriorated with time, and the function at the time of completion (balance of meat texture and taste) was impaired, and a decrease in juiciness and softness of meat was observed. . As described above, although there are many prior arts, some effects are recognized compared with no treatment, but the level is not sufficiently satisfactory, and the reforming effect is improved with improved taste and texture. There is a further need for new materials or efficient reforming methods.

本発明は、食肉の肉質や食感等を改善するための改質剤、及びその製造方法を提供することを目的とする。
本発明者は鋭意検討を重ねた結果、カルシウム塩と米澱粉を併用することにより前記課題を解決できることを見出し、本発明を完成させるに至った。
即ち、本発明は以下の発明を包含する。
(1)カルシウム塩と米澱粉が含有されてなる食肉改質剤であって、食肉改質剤におけるカルシウム塩の含有量が、食肉改質剤に含有されている米澱粉1gに対し、カルシウム換算で0.00065〜0.14gである食肉改質剤。
(2)カルシウム塩が、乳酸カルシウム又は塩化カルシウムである(1)記載の食肉改質剤。
(3)さらに、炭酸塩が含有されてなる(1)又は(2)記載の食肉改質剤。
(4)炭酸塩が炭酸ナトリウムである(3)記載の食肉改質剤。
(5)食肉100gあたり、米澱粉を0.25〜10g、及びカルシウム塩をカルシウム換算で、0.0033〜0.26g食肉に添加し、処理することを特徴とする食肉加工品の製造方法。
(6)カルシウム塩が、乳酸カルシウム又は塩化カルシウムである(5)記載の製造方法。
(7)さらに、食肉100gあたり、炭酸塩を0.1〜1g食肉に添加し、処理することを特徴とする(5)又は(6)記載の製造方法。
(8)炭酸塩が炭酸ナトリウムである(7)記載の製造方法。
本発明により筋肉繊維感を保持した状態で筋肉組織の過剰分解も無く、結合組織を軟化し、適度な保水性と油脂感を兼ね備えたジューシーな品質の食肉が提供される。本発明の食感改質剤により処理された食肉は、微粒な米澱粉が肉内部に浸透し加熱時の肉汁を保持し、かつカルシウム塩にて肉筋線維に内在する脂を溶出させることで肉本来の味・食感を保持したまま苦味を生じることなく適度にジューシー感が付与され、好ましい品質を付与することができる。また、米澱粉とカルシウム塩を併用することで、脂が溶出された筋線維に効率的に米澱粉が入り込むことで、相乗効果が得られる。さらに、タンパク質の溶出効果の高い炭酸塩を用いることで、より相乗効果が得られる。本発明の方法によれば、室温、低温にて食肉を処理することができる。また、従来の肉軟化プロテアーゼ製剤のように食肉を過剰軟化させたり、アルカリ製剤のように組織を均質化させたり、有機酸製剤のように酸味を生じることなく、適度な食感に留め、またその状態を維持することができる。このため、改質剤の添加量や処理時間等を厳密に制御しなくても適度なジューシー感を有する食肉を得ることができる。よって本発明により、低品質の食肉を呈味的・食感的に優れた肉に改質することができる。
以下に本発明について詳細に説明する。
本発明の食肉改質剤は、米澱粉とカルシウム塩が配合されているものである。
米澱粉は、うるち米、もち米、ジャスミン米、短粒米、長粒米など原料米の品種に特に制限はない。また生澱粉でもよいし、湿熱処理澱粉、高周波処理澱粉等物理的処理を施したものでもよく、またリン酸化デンプン、酢酸デンプン等米澱粉由来の加工澱粉でもよい。米澱粉は、粒径が非常に細かいため、肉の筋束間に浸透しやすく、肉内部に肉汁を保水しやすい。
カルシウム塩としては、無水塩でも水和物でもよいが、水への溶解度の高い乳酸カルシウム、塩化カルシウムが好ましい。特に乳酸カルシウムを用いた場合、肉から脂が溶出しやすくなる。
本発明の食肉改質剤における米澱粉とカルシウム塩の含有比率(重量比)は米澱粉1gに対しカルシウム塩はカルシウム換算で、0.00065〜0.14gが好ましく、0.0018〜0.14gがより好ましく、0.0036〜0.072gがさらに好ましく、0.011〜0.072gが特に好ましい。尚、カルシウム換算とは、カルシウム塩中のカルシウム量を意味し、塩化カルシウム二水和物の場合、分子量が147であるので、塩化カルシウム二水和物1gのカルシウム換算値は40÷147=0.27gとなる。同様に、乳酸カルシウムの場合、六水和物であるため、1g当たり0.13gとなる。
本発明の食肉改質剤は、米澱粉とカルシウム塩が配合されていればよいが、炭酸ナトリウム、炭酸水素ナトリウム、炭酸カリウム、炭酸カルシウム等の炭酸塩が配合されているとより好ましい。炭酸塩により原料肉よりタンパク質が溶出し、品質のさらなる向上が得られるが、特に炭酸ナトリウムが好ましい。
さらに、クエン酸、フィチン酸、フマル酸等の有機酸、グリシン、グルタミン酸ナトリウム(MSG)、アスパラギン酸ナトリウム等のアミノ酸、ショ糖、デキストリン、マルチトール、オリゴ糖等の糖質、プロテアーゼ、マルチトール等糖アルコール、有機酸塩類、食塩、調味料等の他の食品素材が配合されていてもよい。
本発明の食肉加工品の製造方法は、米澱粉とカルシウム塩を食肉に添加し、処理するものであり、さらに炭酸塩を添加するとよりよい。
米澱粉の添加量は、食肉100gあたり、0.25〜10gが好ましく、0.25〜4gがより好ましく、0.25〜2gがさらに好ましい。0.25未満では十分な効果が得られず、10gを超える場合は、効果が得られる場合もあるが、10g添加品と比較して大きなメリットはない。
カルシウム塩の添加量は、食肉100gあたり、カルシウム換算で0.0033〜0.26gが好ましく、0.0065〜0.13gがより好ましく、0.020〜0.065がさらに好ましい。
炭酸塩の添加量は、食肉100gあたり、0.1〜1gが好ましい。
併せて、クエン酸、フィチン酸、フマル酸等の有機酸、グリシン、グルタミン酸ナトリウム(MSG)、アスパラギン酸ナトリウム等のアミノ酸、ショ糖、デキストリン、マルチトール、オリゴ糖等の糖質、プロテアーゼ、マルチトール等糖アルコール、有機酸塩類、食塩等を添加し、処理してもよい。
本発明の食肉改質剤を食肉に添加してもよいし、米澱粉とカルシウム塩を別々に食肉に添加してもよい。各素材の添加の順序は問わない。本発明の食肉改質剤、あるいは米澱粉とカルシウム塩はなるべく食肉全体及びその内部に均一に分散されるようにする。このためには、挽き肉、細切り肉、薄切り肉等に対しては、食肉を食肉改質剤の溶液に漬け込む浸漬法や、食肉改質剤の溶液や粉末を食肉へ噴霧して揉む方法等を採用することができ、ステーキ肉やブロック肉の場合には、食肉改質剤の溶液を注射器で内部に注入するインジェクション法や、表面に塗布したりする方法が採用することができる。肉軟化剤を処理した後、または肉軟化剤に肉を浸漬した後にタンブリング処理することも可能である。食肉改質剤の溶液に一定時間浸漬する浸漬法でもよい。
上記のようにして、本発明の食肉改質剤、あるいは米澱粉とカルシウム塩を食肉に添加し、好ましくは、肉全体に十分浸透するように、例えば0〜60℃で、0.05〜48時間放置しておくのが好ましい。あるいは浸漬時間を取らずに、食肉加工/調理直前に本発明の食肉改質剤、あるいは米澱粉とカルシウム塩を食肉に添加し処理する方法も可能である。
処理できる食肉としては食用可能な肉であれば特に限定されるものではなく、例えば、牛肉、豚肉、羊肉、馬肉等の畜肉、鶏、七面鳥、カモ、ガチョウ、アヒル、ウズラ等の家禽類の肉、魚肉等のいずれのものでも処理できるが、輸入牛肉やカタやモモ、スジ、スネの部分等の硬質肉に対して特に効果的である。また、食肉の形状としては特に限定されるものではなく、厚切り肉、薄切り肉、細切り肉、挽き肉等が挙げられる。
本発明の製造方法により改質処理を行った食肉加工品は、例えば、焼く、炒める、揚げる、煮る、蒸すなどの加熱調理によっても過度に硬質化することなく、適度な柔らかさを有し、食しやすいものとなる。また、本発明の食肉改質剤は食肉を過度に軟化させることがないので、例えば、本発明の食肉改質剤を適用した食肉を常温や冷凍、冷蔵、又はレトルト等として長期間保存しても過剰軟化することなく適度な肉の柔らかさやジューシーさが維持され、良好な風味・食感を長期間維持することができる。
An object of this invention is to provide the modifier for improving the meat quality, texture, etc. of meat, and its manufacturing method.
As a result of extensive studies, the present inventor has found that the above problem can be solved by using a calcium salt and rice starch in combination, and has completed the present invention.
That is, the present invention includes the following inventions.
(1) A meat modifier comprising a calcium salt and rice starch, wherein the calcium salt content in the meat modifier is calcium equivalent to 1 g of rice starch contained in the meat modifier. Meat modifier which is 0.00065-0.14g.
(2) The meat modifier according to (1), wherein the calcium salt is calcium lactate or calcium chloride.
(3) The meat modifier according to (1) or (2), further comprising a carbonate.
(4) The meat modifier according to (3), wherein the carbonate is sodium carbonate.
(5) A method for producing a processed meat product, comprising adding 0.25 to 10 g of rice starch and 0.0033 to 0.26 g of calcium salt in terms of calcium per 100 g of meat and processing the meat.
(6) The production method according to (5), wherein the calcium salt is calcium lactate or calcium chloride.
(7) The manufacturing method according to (5) or (6), further comprising adding 0.1 to 1 g of carbonate to 100 g of meat and treating the meat.
(8) The production method according to (7), wherein the carbonate is sodium carbonate.
The present invention provides a juicy quality meat that softens connective tissue without losing excessive muscle tissue while maintaining a feeling of muscle fiber, and has both moderate water retention and oily feeling. Meat treated with the texture modifier of the present invention is that fine rice starch penetrates inside the meat, holds the meat juice during heating, and elutes the fat inherent in the meat muscle fibers with calcium salt While maintaining the original taste and texture of meat, a moderately juicy feeling is imparted without causing bitterness, and a desirable quality can be imparted. Further, by using rice starch and calcium salt in combination, the rice starch efficiently enters the muscle fibers from which the fat is eluted, thereby obtaining a synergistic effect. Further, a synergistic effect can be obtained by using a carbonate having a high protein elution effect. According to the method of the present invention, meat can be processed at room temperature and low temperature. In addition, excessive softening of meat like conventional meat softening protease preparations, homogenization of tissues like alkaline preparations, and no acidity like organic acid preparations, but with a moderate texture, That state can be maintained. For this reason, it is possible to obtain meat having an appropriate juicy feeling without strictly controlling the addition amount of the modifier, the processing time, and the like. Therefore, according to the present invention, low-quality meat can be modified into meat having excellent taste and texture.
The present invention is described in detail below.
The meat modifier of the present invention contains rice starch and calcium salt.
The rice starch is not particularly limited to the varieties of raw rice such as glutinous rice, glutinous rice, jasmine rice, short grain rice, and long grain rice. Moreover, raw starch may be sufficient, what was subjected to physical treatments, such as wet heat-treated starch and high-frequency-treated starch, and may be processed starch derived from rice starch such as phosphorylated starch and acetate starch. Rice starch has a very small particle size, so it easily penetrates between the muscle muscle bundles, and it is easy to retain the juice in the meat.
The calcium salt may be an anhydrous salt or a hydrate, but calcium lactate and calcium chloride having high solubility in water are preferable. In particular, when calcium lactate is used, fat is easily eluted from the meat.
The content ratio (weight ratio) of rice starch and calcium salt in the meat modifier of the present invention is preferably 0.00065 to 0.14 g, 0.0018 to 0.14 g in terms of calcium relative to 1 g of rice starch. Is more preferable, 0.0036 to 0.072 g is more preferable, and 0.011 to 0.072 g is particularly preferable. The calcium conversion means the amount of calcium in the calcium salt. In the case of calcium chloride dihydrate, the molecular weight is 147. Therefore, the calcium conversion value of 1 g of calcium chloride dihydrate is 40 ÷ 147 = 0. .27 g. Similarly, in the case of calcium lactate, it is 0.13 g per g because it is a hexahydrate.
The meat modifier of the present invention only needs to contain rice starch and calcium salt, but more preferably carbonates such as sodium carbonate, sodium bicarbonate, potassium carbonate, and calcium carbonate. Proteins are eluted from the raw meat by the carbonate, and a further improvement in quality is obtained, but sodium carbonate is particularly preferred.
Furthermore, organic acids such as citric acid, phytic acid, fumaric acid, amino acids such as glycine, sodium glutamate (MSG), sodium aspartate, saccharides such as sucrose, dextrin, maltitol, oligosaccharide, protease, maltitol, etc. Other food materials such as sugar alcohols, organic acid salts, salt and seasonings may be blended.
In the method for producing a processed meat product of the present invention, rice starch and calcium salt are added to meat and processed, and it is better to add carbonate.
The amount of rice starch added is preferably 0.25 to 10 g, more preferably 0.25 to 4 g, and even more preferably 0.25 to 2 g per 100 g of meat. If it is less than 0.25, a sufficient effect cannot be obtained, and if it exceeds 10 g, an effect may be obtained, but there is no significant merit compared to a 10 g added product.
The amount of calcium salt added is preferably 0.0033 to 0.26 g in terms of calcium per 100 g of meat, more preferably 0.0065 to 0.13 g, and still more preferably 0.020 to 0.065.
The amount of carbonate added is preferably 0.1 to 1 g per 100 g of meat.
In addition, organic acids such as citric acid, phytic acid, fumaric acid, amino acids such as glycine, sodium glutamate (MSG), sodium aspartate, saccharides such as sucrose, dextrin, maltitol, oligosaccharide, protease, maltitol An isosugar alcohol, an organic acid salt, sodium chloride or the like may be added and treated.
The meat modifier of the present invention may be added to meat, or rice starch and calcium salt may be added separately to meat. The order of addition of each material is not ask | required. The meat modifier of the present invention, or rice starch and calcium salt should be dispersed uniformly throughout the meat as much as possible. For this purpose, for minced meat, shredded meat, thin sliced meat, etc., a dipping method in which meat is soaked in a solution of meat modifier, a method in which a solution or powder of meat modifier is sprayed on the meat, etc. In the case of steak meat or block meat, an injection method in which a solution of a meat modifier is injected into the inside with a syringe or a method of applying to the surface can be employed. It is also possible to perform a tumbling treatment after treating the meat softener or after immersing the meat in the meat softener. An immersion method of immersing in a solution of meat modifier for a certain time may be used.
As described above, the meat modifier of the present invention, or rice starch and calcium salt is added to the meat, and preferably 0.05 to 48, for example, at 0 to 60 ° C. so as to sufficiently penetrate the whole meat. It is preferable to leave for a period of time. Alternatively, it is possible to add the meat modifier of the present invention or the rice starch and calcium salt to the meat and process it immediately before the meat processing / cooking without taking the soaking time.
The meat that can be processed is not particularly limited as long as it is edible. For example, meat such as beef, pork, lamb, and horse meat, poultry such as chicken, turkey, duck, goose, duck, and quail. It can be processed with any kind of fish meat, etc., but it is particularly effective for imported beef and hard meat such as kata, peach, stripes, and shin. Moreover, it is not specifically limited as a shape of meat, Thick cut meat, thin sliced meat, shredded meat, ground meat etc. are mentioned.
The processed meat product subjected to the modification treatment by the production method of the present invention has an appropriate softness without being excessively hardened by, for example, cooking such as baking, frying, frying, boiling, steaming, etc. It becomes easy to eat. In addition, since the meat modifier of the present invention does not excessively soften the meat, for example, the meat to which the meat modifier of the present invention is applied is stored for a long period of time as normal temperature, frozen, refrigerated, retort, etc. In addition, moderate softness and juiciness of meat can be maintained without excessive softening, and good flavor and texture can be maintained for a long time.

図1は、本発明の実験例1に係る、肉への澱粉の浸透状況を示す図である。
図2は、本発明の実施例7に係る、肉のCLSM写真を示す図である。
図3は、本発明の実施例8に係る、肉のSEM写真を示す図である。
図4は、本発明の実施例9に係る、肉の脂溶出量を示す図である。
FIG. 1 is a diagram showing the state of starch penetration into meat according to Experimental Example 1 of the present invention.
FIG. 2 is a view showing a CLSM photograph of meat according to Example 7 of the present invention.
FIG. 3 is a view showing an SEM photograph of meat according to Example 8 of the present invention.
FIG. 4 is a diagram showing the fat elution amount of meat according to Example 9 of the present invention.

以下に実験例及び実施例を挙げ、本発明をさらに詳しく説明する。本発明は、この実施例により何ら限定されない。
実験例1
米澱粉が肉内部にどの程度浸透しているかを確認するため、浸漬後の澱粉をヨウ素にて染色することで浸透状態を観察した。
鶏もも肉を、対肉3%量のうるち米澱粉「ファインスノウ」(上越スターチ社製)または馬鈴薯澱粉(松谷化学工業社製)を懸濁させた対肉30%量の市水に3時間浸漬した。次に、水250gに対し、ヨウ素0.3gとヨウ化カリウム1gを溶解し、ヨウ素液を調製した。そして、浸漬処理後の肉を、包丁を用いて適当な大きさにカットし、ヨウ素液に10分間浸漬させた後、マイクロスコープVH−S30(株式会社キーエンス)にて観察を行った。
結果を図1に示す。馬鈴薯澱粉では肉表層部にのみ付着しているのに対し、米澱粉では肉全体にほぼ均一に浸透している様子が見られた。これは、米澱粉の粒径が非常に細かいため、筋束間に浸透しやすいものと考えられる。また、それにより、肉内部に肉汁を保水しやすくなると考えられる。
Hereinafter, the present invention will be described in more detail with reference to experimental examples and examples. The present invention is not limited in any way by this example.
Experimental example 1
In order to confirm how much the rice starch penetrates into the meat, the penetration state was observed by dyeing the starch after immersion with iodine.
Chicken thighs were soaked for 3 hours in 30% anti-meat city water in which 3% anti-glutinous rice starch “Fine Snow” (manufactured by Joetsu Starch Co., Ltd.) or potato starch (manufactured by Matsutani Chemical Industry Co., Ltd.) was suspended. . Next, 0.3 g of iodine and 1 g of potassium iodide were dissolved in 250 g of water to prepare an iodine solution. Then, the meat after the immersion treatment was cut into an appropriate size using a knife and immersed in an iodine solution for 10 minutes, and then observed with a microscope VH-S30 (Keyence Corporation).
The results are shown in FIG. In potato starch, it adhered only to the surface layer of meat, while rice starch was seen to penetrate almost uniformly throughout the meat. This is probably because rice starch has a very small particle size and is likely to penetrate between muscle bundles. Moreover, it is thought that it becomes easy to hold | maintain the gravy inside the meat.

市水60gに馬鈴薯澱粉(松谷化学工業社製)、うるち米澱粉「ファインスノウ」(上越スターチ社製)、コーンスターチ「コーンスターチY」(J−オイルミルズ社製)、タピオカ澱粉「精製乾燥殺菌タピオカ」(松谷化学工業(株))のうち1種類と乳酸カルシウム(武蔵野化学研究所社製)を溶解させ、ブラジル産鶏もも肉200gを冷蔵で2時間浸漬した。その後、液を切り、薄力粉をまぶし、170℃の油にて5分間揚げることで、唐揚げを調製した。米澱粉および乳酸カルシウムの量は表1の通りである。官能評価および歩留算出結果を表1に示す。官能評価は、保水性、油脂感、ジューシー感に関して、各種澱粉および乳酸カルシウムのいずれも添加されずに調製された対照区分を0点として、−5点から5点までの評点法にて評価人数4名で行った。なお、保水性とは水分を多く保持していると感じること、油脂感とは脂を多く保持していると感じること、ジューシー感とは水分と脂のバランスが好ましく、肉本来の味を有し、ジューシーに感じることを意味する。また、歩留は浸漬前後での重量増加率に、揚げ前後での重量減量率を乗じたものとした。総合評価は、官能評価の3項目がいずれも1.75点以上をA、3項目が1.0点以上1.75点未満をB、A、B以外をCとした。また各素材を併用した試験区官能評価の点数が、各素材を単体にて使用した場合と比較し、官能評価の項目同士の加算値よりも高い点数を示した際は、相乗効果有りとした。
米澱粉のみの添加では、他の澱粉と比較して、大きな歩留向上は認められず、油脂感やジューシー感の向上も少なかった。しかし、乳酸カルシウムと併用することで、他の澱粉よりも大きな歩留向上が認められ、また、米澱粉のみや乳酸カルシウムのみでの品質向上効果の加算を遥かに上回る、相乗的な品質向上効果を示した。
Potato starch (manufactured by Matsutani Chemical Co., Ltd.), Urushi rice starch “Fine Snow” (manufactured by Joetsu Starch Co., Ltd.), corn starch “Corn Starch Y” (manufactured by J-Oil Mills Co., Ltd.), tapioca starch “refined and dried sterilized tapioca” Matsutani Chemical Industry Co., Ltd.) and calcium lactate (Musashino Chemical Laboratory Co., Ltd.) were dissolved, and 200 g of Brazilian chicken thighs were refrigerated for 2 hours. Thereafter, the liquid was cut, dusted with flour, and fried in oil at 170 ° C. for 5 minutes to prepare fried chicken. The amounts of rice starch and calcium lactate are as shown in Table 1. The sensory evaluation and yield calculation results are shown in Table 1. The sensory evaluation was based on a grading method of -5 to 5 points, with 0 as the control category prepared without adding any starch or calcium lactate, with respect to water retention, oily feeling and juicy feeling. We went with 4 people. It should be noted that water retention means that it feels that it retains a lot of moisture, oily feeling means that it feels that it retains a lot of fat, and juicy feeling means that the balance between moisture and fat is preferable, and it has the original taste of meat. And it means to feel juicy. The yield was obtained by multiplying the weight increase rate before and after immersion by the weight loss rate before and after frying. In the comprehensive evaluation, all three items of sensory evaluation were set to A for 1.75 points or more, and B for items 3 to 1.0 for less than 1.75 points. In addition, when the score of the sensory evaluation using the test materials in combination with each material was higher than the addition value between the sensory evaluation items compared to the case where each material was used alone, there was a synergistic effect. .
When only rice starch was added, no significant yield improvement was observed as compared with other starches, and there was little improvement in oily or juicy feeling. However, when used in combination with calcium lactate, a greater yield improvement than other starches has been observed, and a synergistic quality improvement effect far exceeds the addition of quality improvement with rice starch alone or calcium lactate alone. showed that.

市水60gにうるち米澱粉「ファインスノウ」(上越スターチ社製)と、乳酸カルシウム(武蔵野化学研究所社製)および塩化カルシウム(富田製薬社製)を溶解させ、ブラジル産鶏もも肉200gを冷蔵で2時間浸漬した。その後、液を切り、薄力粉をまぶし、170℃の油にて5分間揚げることで、唐揚げを調製した。米澱粉、乳酸カルシウムおよび塩化カルシウムの量は表2の通りである。実施例1同様の方法で行なった官能評価および歩留算出結果を表2に示す。
乳酸カルシウムと塩化カルシウムはカルシウム換算量が同量であれば、同程度の歩留向上および米澱粉との相乗的な品質向上を示した。
Dissolve glutinous rice starch "Fine Snow" (manufactured by Joetsu Starch Co., Ltd.), calcium lactate (manufactured by Musashino Chemical Laboratory Co., Ltd.) and calcium chloride (manufactured by Tomita Pharmaceutical) in 60 g of city water, and refrigerate 200 g of Brazilian chicken leg 2 Soaked for hours. Thereafter, the liquid was cut, dusted with flour, and fried in oil at 170 ° C. for 5 minutes to prepare fried chicken. Table 2 shows the amounts of rice starch, calcium lactate and calcium chloride. Table 2 shows the results of sensory evaluation and yield calculation performed in the same manner as in Example 1.
Calcium lactate and calcium chloride showed similar yield improvement and synergistic quality improvement with rice starch if the calcium equivalent amount was the same.

市水60gにうるち米澱粉「ファインスノウ」(上越スターチ社製)と乳酸カルシウム(武蔵野化学研究所社製)を溶解させ、ブラジル産鶏もも肉200gを冷蔵で2時間浸漬した。その後、液を切り、薄力粉をまぶし、170℃の油にて5分間揚げることで、唐揚げを調製した。肉100重量部当たりの各種澱粉および乳酸カルシウムの量は表3の通りである。実施例同様の方法で行った官能評価および歩留算出結果を表3に示す。
肉重量100あたり米澱粉重量1.8添加時に、乳酸カルシウム重量が0.025〜2(カルシウム換算で0.0033〜0.26)の範囲にて歩留向上および相乗的な品質向上を示した。また、特に乳酸カルシウム重量が0.05〜1(カルシウム換算で0.0065〜0.13)の範囲にて歩留向上および相乗的な品質向上を示した。
Urushi rice starch "Fine Snow" (manufactured by Joetsu Starch Co., Ltd.) and calcium lactate (manufactured by Musashino Chemical Laboratory Co., Ltd.) were dissolved in 60 g of city water, and 200 g of Brazilian chicken leg was soaked in a refrigerator for 2 hours. Thereafter, the liquid was cut, dusted with flour, and fried in oil at 170 ° C. for 5 minutes to prepare fried chicken. Table 3 shows the amounts of various starches and calcium lactate per 100 parts by weight of meat. Table 3 shows the results of sensory evaluation and yield calculation performed in the same manner as in the examples.
Yield improvement and synergistic quality improvement were shown when calcium lactate weight was in the range of 0.025-2 (0.0033-0.26 in terms of calcium) at the time of 1.8 addition of rice starch weight per 100 meat weight. . Moreover, the yield improvement and synergistic quality improvement were shown especially in the range whose calcium lactate weight is 0.05-1 (0.0065-0.13 in conversion of calcium).

市水60gにうるち米澱粉「ファインスノウ」(上越スターチ社製)と乳酸カルシウム(武蔵野化学研究所社製)を溶解させ、ブラジル産鶏もも肉200gを冷蔵で2時間浸漬した。その後、液を切り、薄力粉をまぶし、170℃の油にて5分間揚げることで、唐揚げを調製した。各種澱粉および乳酸カルシウムの量は表4の通りである。実施例1同様の方法で行った官能評価および歩留算出結果を表4に示す。
肉重量100あたり乳酸カルシウム重量が0.05〜1(カルシウム換算で対肉0.0065〜0.13重量%)の場合、米澱粉重量が0.25〜10の範囲にて歩留向上および相乗的な品質向上を示した。
Urushi rice starch "Fine Snow" (manufactured by Joetsu Starch Co., Ltd.) and calcium lactate (manufactured by Musashino Chemical Laboratory Co., Ltd.) were dissolved in 60 g of city water, and 200 g of Brazilian chicken leg was soaked in a refrigerator for 2 hours. Thereafter, the liquid was cut, dusted with flour, and fried in oil at 170 ° C. for 5 minutes to prepare fried chicken. Table 4 shows the amounts of various starches and calcium lactate. Table 4 shows the sensory evaluation and yield calculation results performed in the same manner as in Example 1.
When the calcium lactate weight per 100 meat weight is 0.05 to 1 (0.0065 to 0.13% by weight in terms of calcium), the rice starch weight is in the range of 0.25 to 10 and yield improvement and synergy Quality improvement.

市水60gにうるち米澱粉「ファインスノウ」と乳酸カルシウム(武蔵野化学研究所社製)および各種塩、炭酸ナトリウム(大東化学社製)、炭酸水素ナトリウム(旭硝子社製)、炭酸カリウム(旭硝子社製)、リン酸三ナトリウム(ソーダニッカ社製)、ポリリン酸ナトリウム(千代田商工社製)を溶解させ、ブラジル産鶏もも肉200gを冷蔵で2時間浸漬した。その後、液を切り、薄力粉をまぶし、170℃の油にて5分間揚げることで、唐揚げを調製した。各種澱粉および乳酸カルシウムの量は表5の通りである。官能評価および歩留算出結果を表5に示す。官能評価は、保水性、油脂感、ジューシー感に関して、各種澱粉および乳酸カルシウムのいずれも添加されずに調製された対照区分を0点として、−5点から5点までの評点法にて評価人数4名で行った。また繊維感のあるものを○、ないものを×とした。なお、繊維感とは繊維が分解されず、均一な食感をではなく、肉の筋繊維と並行した面と垂直な面では異なる食感を有することを意味する。また、歩留は浸漬前後での重量増加率に、揚げ前後での重量減量率を乗じたものとした。繊維感があり、保水性、油脂感、ジューシー感の3項目がいずれも1.75点以上をA、繊維感があり、3項目が1.0点以上1.75点未満をB、A、B以外をCとした。また各素材を併用した試験区官能評価の点数が、各素材を単体にて使用した場合と比較し、官能評価の項目同士の加算値よりも高い点数を示した際は、相乗効果有りとした。
米澱粉、乳酸カルシウム、炭酸塩を併用することで、米澱粉と乳酸カルシウムの併用や、炭酸塩のみでの品質向上効果の加算を遥かに上回る、相乗的な品質向上効果を示した。
In 60 g of city water, glutinous rice starch "Fine Snow", calcium lactate (Musashino Chemical Laboratory Co., Ltd.) and various salts, sodium carbonate (Daito Chemical Co., Ltd.), sodium bicarbonate (Asahi Glass Co., Ltd.), potassium carbonate (Asahi Glass Co., Ltd.) Trisodium phosphate (manufactured by Sodanikka Co., Ltd.) and sodium polyphosphate (manufactured by Chiyoda Shoko Co., Ltd.) were dissolved, and 200 g of Brazilian chicken leg was soaked in a refrigerator for 2 hours. Thereafter, the liquid was cut, dusted with flour, and fried in oil at 170 ° C. for 5 minutes to prepare fried chicken. Table 5 shows the amounts of various starches and calcium lactate. Table 5 shows sensory evaluation and yield calculation results. The sensory evaluation was based on a grading method of -5 to 5 points, with 0 as the control category prepared without adding any starch or calcium lactate, with respect to water retention, oily feeling and juicy feeling. We went with 4 people. In addition, those having a feeling of fiber were marked with ◯, and those having no fiber feeling were marked with ×. The fiber feeling means that the fibers are not decomposed and do not have a uniform texture, but have a different texture on a plane perpendicular to the plane parallel to the muscle fibers of meat. The yield was obtained by multiplying the weight increase rate before and after immersion by the weight loss rate before and after frying. There is a feeling of fiber, all three items of water retention, oily feeling, and juicy feeling are 1.75 points or more, and there is a fiber feeling, three items are 1.0 points or more and less than 1.75 points B, A, C other than B was designated. In addition, when the score of the sensory evaluation using the test materials in combination with each material was higher than the addition value between the sensory evaluation items compared to the case where each material was used alone, there was a synergistic effect. .
The combined use of rice starch, calcium lactate, and carbonate showed a synergistic quality improvement effect that far exceeded the combined use of rice starch and calcium lactate and the addition of quality improvement effect with carbonate alone.

市水60gにうるち米澱粉「ファインスノウ」(上越スターチ社製)と乳酸カルシウム(武蔵野化学研究所社製)および炭酸ナトリウム(大東化学社製)を溶解させ、ブラジル産鶏もも肉200gを冷蔵で2時間浸漬した。その後、液を切り、薄力粉をまぶし、170℃の油にて5分間揚げることで、唐揚げを調製した。米澱粉、乳酸カルシウム、炭酸ナトリウムの量は表6の通りである。実施例5と同様の方法で行った官能評価および歩留算出結果を表6に示す。肉重量100あたり乳酸カルシウム重量が0.05〜1、米澱粉重量が0.25〜10、炭酸ナトリウム重量が0.1〜1の範囲にて歩留向上および相乗的な品質向上を示した。
Dissolve glutinous rice starch "Fine Snow" (manufactured by Joetsu Starch Co., Ltd.), calcium lactate (manufactured by Musashino Chemical Laboratory) and sodium carbonate (manufactured by Daito Chemical Co., Ltd.) in 60 g of city water, and refrigerate 200 g of Brazilian chicken leg for 2 hours. Soaked. Thereafter, the liquid was cut, dusted with flour, and fried in oil at 170 ° C. for 5 minutes to prepare fried chicken. Table 6 shows the amounts of rice starch, calcium lactate and sodium carbonate. Table 6 shows the sensory evaluation and yield calculation results performed in the same manner as in Example 5. Yield improvement and synergistic quality improvement were shown when calcium lactate weight per meat weight 100 was 0.05 to 1, rice starch weight was 0.25 to 10, and sodium carbonate weight was 0.1 to 1.

乳酸Caの脂溶出効果を確認するため、CLSM(共焦点レーザー走査型顕微鏡)を用いて、肉内部での脂の分布を確認した。
市水60gにうるち米澱粉「松谷ききょう」(松谷化学工業社製)3.5g、炭酸ナトリウム(大東化学社製)0.6g、乳酸カルシウム(武蔵野化学研究所社製)0.5gを溶解させ、ブラジル産鶏もも肉200gを冷蔵で3時間浸漬した。また、乳酸カルシウムを添加しない浸漬液に浸漬した肉及び市水に浸漬した肉を対照とした。
肉のタンパクと脂肪を染色するため、超音波カッター(スズキ株式会社SUW−30CMH)を用いて、肉の中心部から薄い切片を作製した。染色にはNile Red(SIGMA)とTRITC Dye(SIGMA)を用いた。
染色液に切片を入れ、冷蔵庫で一晩遮光しながら放置した後、サンプルをプレパラートに乗せ、CLSMで観察した。
結果を図2に示す。米澱粉及び炭酸Naを添加した試料は、市水浸漬品と大差はなかったが、乳酸Ca添加品においては筋束内においても脂が多く内在している様子が観察された。よって、脂の溶出抑制に乳酸Caが大きく寄与していることが示された。
In order to confirm the fat elution effect of lactic acid Ca, the distribution of fat inside the meat was confirmed using CLSM (confocal laser scanning microscope).
In 60 g of city water, 3.5 g of glutinous rice starch “Matsuya Kikyo” (manufactured by Matsutani Chemical Co., Ltd.), 0.6 g of sodium carbonate (manufactured by Daito Chemical Co., Ltd.), 0.5 g of calcium lactate (manufactured by Musashino Chemical Laboratory) are dissolved, 200g of Brazilian chicken leg was soaked for 3 hours in a refrigerator. Moreover, the meat immersed in the immersion liquid which does not add calcium lactate and the meat immersed in the city water were used as controls.
In order to stain the protein and fat of meat, a thin slice was prepared from the center of the meat using an ultrasonic cutter (Suzuki Corporation SUW-30CMH). Nile Red (SIGMA) and TRITC Dye (SIGMA) were used for staining.
A section was placed in the staining solution and allowed to stand in a refrigerator overnight while being shielded from light, and then the sample was placed on a preparation and observed with CLSM.
The results are shown in FIG. The sample to which rice starch and sodium carbonate were added was not much different from the product soaked in city water, but in the product added with lactic acid Ca, it was observed that a large amount of fat was contained in the muscle bundle. Thus, it was shown that Ca lactate greatly contributed to the suppression of fat elution.

唐揚げの皮裏にある脂層が乳酸Caの脂溶出効果にてどのように変化するかを確認するため、SEM(走査型電子顕微鏡)を用いて観察を行った。
市水60gにうるち米澱粉「松谷ききょう」(松谷化学工業社製)3.5g、炭酸ナトリウム(大東化学社製)0.6g、乳酸カルシウム(武蔵野化学研究所社製)0.5gを溶解させ、ブラジル産鶏もも肉200gを冷蔵で3時間浸漬した。その後、液を切り、薄力粉をまぶし、170℃の油にて5分間揚げることで、唐揚げを調製した。尚、市水に浸漬した肉を対照とした。
各試料は、包丁などを用いて適当な大きさに成型した後、薄刃のカミソリにて1mm角に切断した。1mm角のサンプルを1%グルタルアルデヒド溶液に90分浸漬後、2%四酸化オスミウム溶液に90分浸漬させ、2段階の化学固定を行った。固定した試料はよく洗浄し、30,50,60,70,80,90,95,100%のエタノール系列に順次20分浸漬し脱水した。脱水を完全に行うため100%エタノールでの浸漬は2回行った。脱水後の試料は、一度酢酸イソアミルに浸漬(15分×2回)させ、試料内部のエタノールを酢酸イソアミルに置換させた後に二酸化炭素による臨界点乾燥法によって乾燥させた。乾燥後の試料を真鍮製の試料台にカーボン両面テープで貼り付け、オスミウムコーティング(設定約10nm)した。
前処理を行った試料を以下の条件にて観察した。
・SEM機種:電界放射型SEM(JSM6700F)
・加速電圧:3kv、WD:3mm(5万倍以上の観察時)もしくは8mm(5万倍未満の観察)
・照射電流設定:7(5万倍以上の観察時)もしくは6(5万倍未満の観察)
結果を図3に示す。対照の市水浸漬品では脂層が観察されたが、乳酸カルシウム添加品では糸状の骨格構造のみ観察された。これは脂が溶出し、残ったコラーゲンであると考えられる。以上結果より、乳酸カルシウムは皮裏の脂層に大きな効果があることが示された。
In order to confirm how the fat layer on the back of the fried chicken is changed by the fat elution effect of lactic acid Ca, observation was performed using an SEM (scanning electron microscope).
In 60 g of city water, 3.5 g of glutinous rice starch “Matsuya Kikyo” (manufactured by Matsutani Chemical Co., Ltd.), 0.6 g of sodium carbonate (manufactured by Daito Chemical Co., Ltd.), 0.5 g of calcium lactate (manufactured by Musashino Chemical Laboratory) are dissolved, 200g of Brazilian chicken leg was soaked for 3 hours in a refrigerator. Thereafter, the liquid was cut, dusted with flour, and fried in oil at 170 ° C. for 5 minutes to prepare fried chicken. The control was meat immersed in city water.
Each sample was molded into an appropriate size using a knife or the like, and then cut into 1 mm squares with a thin blade razor. A 1 mm square sample was immersed in a 1% glutaraldehyde solution for 90 minutes and then immersed in a 2% osmium tetroxide solution for 90 minutes to perform two-stage chemical fixation. The fixed sample was thoroughly washed and dehydrated by sequentially immersing in an ethanol series of 30, 50, 60, 70, 80, 90, 95, and 100% for 20 minutes. In order to perform dehydration completely, immersion in 100% ethanol was performed twice. The dehydrated sample was once immersed in isoamyl acetate (15 minutes × 2 times), and the ethanol in the sample was replaced with isoamyl acetate, and then dried by a critical point drying method using carbon dioxide. The dried sample was attached to a brass sample table with carbon double-sided tape and osmium-coated (setting about 10 nm).
The pretreated sample was observed under the following conditions.
・ SEM model: Field emission SEM (JSM6700F)
・ Acceleration voltage: 3 kv, WD: 3 mm (when observing 50,000 times or more) or 8 mm (observing less than 50,000 times)
・ Irradiation current setting: 7 (when observing more than 50,000 times) or 6 (observing less than 50,000 times)
The results are shown in FIG. In the control water-immersed product, a fat layer was observed, but in the calcium lactate-added product, only a thread-like skeleton structure was observed. This is thought to be the collagen from which the fat eluted and remained. From the above results, it was shown that calcium lactate has a great effect on the fat layer on the back of the skin.

乳酸Caによる脂溶出効果を確認するため、実施例8記載の方法で調製した唐揚げの脂溶出量を測定した。
唐揚げ肉部50gと湯150gをミキサーにて粉砕し、クロロホルムメタノール(クロロホルム:メタノール=1:1)にて抽出を行い、固形分除去後、乾燥することで脂重量を測定した。
結果を図4に示す。乳酸カルシウム添加により、脂溶出量が増加していることがわかった。これは、実施例7のCLSMおよび実施例8のSEM観察結果に見られるように、筋繊維や、鶏皮の裏に多く付着している脂層から脂が溶出したものと考えられる。
In order to confirm the fat elution effect of lactic acid Ca, the fat elution amount of fried chicken prepared by the method described in Example 8 was measured.
50 g of deep-fried meat and 150 g of hot water were pulverized with a mixer, extracted with chloroform methanol (chloroform: methanol = 1: 1), solid content was removed, and the fat weight was measured by drying.
The results are shown in FIG. It was found that the fat elution amount increased with the addition of calcium lactate. As can be seen from the CLSM of Example 7 and the SEM observation results of Example 8, it is considered that the fat was eluted from the muscle fiber and the fat layer adhering to the back of the chicken skin.

本発明によると、食肉の肉質や食感等を改善することができるため、本発明は食品分野において極めて有用である。   According to the present invention, since the meat quality and texture of meat can be improved, the present invention is extremely useful in the food field.

Claims (7)

カルシウム塩と米澱粉と炭酸塩が含有されてなる食肉改質剤であって、食肉改質剤におけるカルシウム塩の含有量が、食肉改質剤に含有されている米澱粉1gに対し、カルシウム換算で0.00065〜0.14gである食肉改質剤。 A meat modifier comprising calcium salt, rice starch and carbonate , wherein the calcium salt content in the meat modifier is calcium equivalent to 1 g of rice starch contained in the meat modifier. Meat modifier which is 0.00065-0.14g. カルシウム塩が、乳酸カルシウム又は塩化カルシウムである請求の範囲第1項記載の食肉改質剤。   The meat modifier according to claim 1, wherein the calcium salt is calcium lactate or calcium chloride. 炭酸塩が炭酸ナトリウムである請求の範囲第1又は2項記載の食肉改質剤。   The meat modifier according to claim 1 or 2, wherein the carbonate is sodium carbonate. 食肉100gあたり、米澱粉を0.25〜10g、及びカルシウム塩をカルシウム換算で、0.0065〜0.13g食肉に添加し、処理することを特徴とする食肉加工品の製造方法であって、カルシウム塩の添加量が、米澱粉1gに対し、カルシウム換算で0.00065〜0.14gである製造方法。   A method for producing a processed meat product, characterized in that, per 100g of meat, 0.25-10g of rice starch and calcium salt in terms of calcium are added to 0.0065-0.13g of meat and processed. The manufacturing method whose addition amount of calcium salt is 0.00065-0.14g in conversion of calcium with respect to 1g of rice starch. カルシウム塩が、乳酸カルシウム又は塩化カルシウムである請求の範囲第4項記載の製造方法。   The production method according to claim 4, wherein the calcium salt is calcium lactate or calcium chloride. さらに、食肉100gあたり、炭酸塩を0.1〜1g食肉に添加し、処理することを特徴とする請求の範囲第4又は5項記載の製造方法。   Furthermore, the manufacturing method of Claim 4 or 5 characterized by adding 0.1 to 1g of carbonate to meat per 100g of meat, and processing. 炭酸塩が炭酸ナトリウムである請求の範囲第6項記載の製造方法。
The production method according to claim 6, wherein the carbonate is sodium carbonate.
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