JP5974230B2 - Magnetic stand - Google Patents

Description

  The present invention relates to a magnetic stand, and in particular, the container can be cooled and the magnetic fine particles can be quickly recovered by forming the base from a metal and bringing the tip of the magnetic means into close contact with the tapered portion of the container. Related to new improvements.

Conventionally, a method of immobilizing chemical substances, nucleic acids, antibodies, proteins, etc. using magnetic fine particles as a carrier, and magnetically separating biological substances such as proteins, nucleic acids, cells, etc. that specifically bind to them from a solution has been used. Yes.
In order to separate the magnetic fine particles to which the biological substance is bound from the solution, a stand having a permanent magnet in which a container such as a microtube or a test tube can be arranged is used.
The configuration (not shown) described in Patent Document 1 is a magnet stand in which a plurality of semi-cylindrical opening grooves into which test tubes are inserted are provided on a base and magnets are arranged perpendicular to the opening grooves. The base material is preferably Teflon (registered trademark) resin which is easy to keep clean and easy to process.

  The magnetic stand of Patent Document 2 shown in FIGS. 4 to 9 includes a back plate having a semi-cylindrical or semi-conical groove, a permanent magnet embedded in parallel to the back surface of the groove, and a lower portion of the back plate. A magnetic stand having a bottom plate disposed vertically to the back plate, and made of elastic materials facing each other so as to sandwich the groove in the front surface of the back plate and swell forward. A magnetic stand comprising an arcuate arm. The arm and back plate are preferably made of nylon resin in terms of integral molding and workability.

  That is, in the case of the magnetic stand of Patent Document 2 described above with reference to FIGS. 4 to 9, five sample-compatible magnetic stands are illustrated. As shown in FIGS. 4 and 5, a semi-cylindrical groove 2 is provided on the back plate 1, a magnet embedding groove 3 is formed so as to be parallel to the back portion, and a test tube or a microtube is formed on the bottom plate 4. The container fixing hole 6 is provided so that the bottom of the container can be fixed.

  Next, the magnetic stand 10 as described above was integrally molded with the same mold using nylon resin as a material. After molding, a plate-like neodymium magnet 3A was embedded from above, and an adhesive was poured into the top to fix it. The arm 5 was designed to be almost the same as or slightly larger than the outer diameter of the test tube to be used and the diameter of the ellipse short or circle formed by the groove 3 and the arm 5. The protruding portion 7 at the tip of the arm 5 is designed to be slightly inside the outer diameter of the container, and is designed to come into contact with the container to be fixed at a point.

  In particular, FIGS. 6 to 8 show possible preferred arm 5 shapes. The shape of the arm 5 is appropriately selected depending on the shape and size of the object to be fixed. The protruding portion at the tip of the arm 5 in FIGS. 6 and 7 is for making the pressing force substantially constant even when the outer diameter of the container to be fixed is slightly changed, as described above. Furthermore, in the case of fixing firmly, a flat structure as shown in FIG. 8 is suitable. In such a case, the inner diameter of the arm 5 is designed to be slightly smaller than the outer diameter of the container.

  Further, in the case of the above-described Patent Document 3 (not shown), a top plate in which an insertion hole for inserting the container from above is formed, and a mounting for placing the bottom portion of the container in correspondence with the insertion hole of the upper plate. A bottom plate formed with a mounting recess and facing the top plate at a predetermined distance; a pair of side plates facing the top plate and the bottom plate; and the top plate and the bottom plate And a magnetic plate provided with a magnet member arranged in an upright position, and a slit is formed in one of the side plates, and the magnetic plate is attached and detached between the top plate and the bottom plate through the slit. It is a magnetic stand that is freely mounted, and the top plate, the bottom plate, the side plate, and the magnet plate are preferably made of a resin that can be integrally molded.

International Publication No. 90/14891 Japanese Patent No. 4,214,253 Japanese Patent No. 4,089,210

Since the conventional magnetic stand is configured as described above, the following problems exist.
In general, in order to separate a biological material while maintaining its biological activity, it is necessary to operate the solution in which the biological material is dissolved at 10 ° C. or lower, and it is necessary to use a magnetic stand on ice. There is.
However, since the conventional magnetic stand is made of resin, the solution cannot be cooled to 10 ° C. or lower even when placed on ice, and it was necessary to be careful that the operation is performed while maintaining physiological activity.
In addition, in a conventional magnetic stand in which a container is installed in a semi-cylindrical groove or a magnetic stand in which a magnetic plate is detachable, the solution cannot be cooled depending on the shape of the container because the container and the magnetic stand do not adhere to each other. Moreover, since the container and the magnet are separated from each other, it takes time to collect the magnetic fine particles. In particular, in the case of the comparative example shown in FIG. 9, a gap G was generated between the tapered portion 31 of the container 11 and the end face of the magnetic means 3A, and the magnetic force was not sufficiently exhibited.
Therefore, the present invention is to provide a magnetic stand that can sufficiently cool a solution in a container when used on ice and can quickly separate magnetic fine particles in the solution.

A magnetic stand according to the present invention has a holding base for inserting a container and made of metal, and magnetic means provided on the base and corresponding to the lower part of the container and made of a permanent magnet or an electromagnet. A positioning recess provided at a lower portion of the base for positioning the lower portion of the container, wherein the magnetic tip of the magnetic means is in close contact with the tapered portion of the container, and the magnetic means is elongated. in the configuration and magnetized in the direction, and the plunger hole located in the upper part of the magnetic means provided on the base, for urging the sides of the container provided in the plunger for hole It is the structure which provided the plunger.

Since the magnetic stand according to the present invention is configured as described above, the following effects can be obtained.
That is, a base made of metal having a plurality of holding holes for inserting a container, magnetic means made of a permanent magnet or an electromagnet provided on the base and corresponding to the lower part of the container, and the base A positioning recess provided at a lower portion of the base for positioning the lower portion of the container, a plunger hole provided at the upper stage of the magnetic means, and provided in the plunger hole. A plunger for biasing the side, and the metal tip of the magnetic means is in close contact with the tapered portion of the container, so that the metal base comes into contact with and in close contact with the container. Even when the temperature is high, the solution in the container can be cooled and held at 2 to 8 ° C. by placing the base on ice, and the experimental operation can be performed while maintaining the physiological activity.
Furthermore, the magnetic means is magnetized in the longitudinal direction, and the magnetic tip of the magnetic means is in close contact with the tapered portion of the container, so that the magnetic substance bound to the biological material dispersed in the solution is bound. Fine particles can be collected quickly.

It is a top view with a partial cross section which shows the magnetic stand by this invention. It is a front view with a partial cross section of FIG. It is sectional drawing of the side surface of FIG. It is a top view which shows the conventional magnetic stand. It is an expanded sectional view of the principal part of FIG. It is a top view which shows the other form of the arm of FIG. It is a top view which shows the other form of the arm of FIG. It is a top view which shows the other form of the arm of FIG. It is sectional drawing which installed the container in FIG.

  An object of this invention is to provide the magnetic stand which can cool a container and can collect | recover magnetic particles rapidly by forming a base with a metal and sticking a magnetic means to a container.

Hereinafter, preferred embodiments of a magnetic stand according to the present invention will be described with reference to the drawings.
A reference numeral 1 in FIGS. 1 to 3 is a base made of a well-known metal having high thermal conductivity, such as aluminum, copper, iron, titanium, nickel, or an alloy thereof. The upper plate portion 20 of the base 1 is formed with a plurality of holding holes 6 arranged in a straight line at a predetermined interval.

A concave portion 22 is formed between the upper plate portion 20 and the lower plate portion 21 of the base 1. The concave portion 22 is a hollow portion whose front side is open. Corresponding to the holes 6, grooves 2 having a semicircular cross section are formed.
The lower plate portion 21 is located at the bottom of each groove 2, the holding hole 6 and the groove 2 communicate with each other, and the upper surface 50 of the lower plate portion 21 is formed with the concave portion 22 and the bottom of the groove 2. Become.

The upper plate portion 20 is provided with a container 11 which is inserted into the holding hole 6 and the concave portion 22 and contains a solution, and is along a hole direction 24 in a direction perpendicular to the axial direction 23 of the container 11. A plunger hole 25 is formed.
The plunger 12 inserted or screwed into the plunger hole 25 is disposed in a direction orthogonal to the axial direction 23, and the distal end 12 a of the plunger 12 biases the side portion of the container 11. So that the container 11 is positioned in the holding hole 6.

On the back side of each groove 2 on the back surface 22a of the concave portion 22 of the base 1, a magnet means hole 26 is formed below the plunger hole 25 and in a direction not parallel to the hole direction 24. In the magnet means hole 26, a rod-like shape and a longitudinal magnetic means 4 are inserted and disposed along the longitudinal direction A.
Since the magnet means hole 26 is inclined with respect to the horizontal surface of the base 1, the magnet tip 3Aa of the magnetic means 3A inserted into the magnet means hole 26 is a tapered portion at the bottom of the container 11. 31 is in close contact with.
The lower part, that is, the tip 11a of the container 11 is positioned so as to enter the positioning recess 51 formed on the upper surface 50 of the lower plate part 21.

  The inner surface 2a of each groove 2 formed on the back surface 22a of the concave portion 22 communicates with the magnetic means hole 26, and the inner surface 2a of the groove 2 is shown in the sectional view of FIG. Like the taper portion 31, the taper portion 31 is formed in a tapered shape so as to coincide with each other.

  Therefore, in the magnetic stand 30 described above, when the container 11 having the tapered portion 31 at the tip end is inserted into each holding hole 6, the tip 11 a of the container 11 contacts the positioning recess 51 of the lower plate portion 21. Positioned in contact with each other and advancing the plunger 12, the side portion of the container 11 is urged to one side in the holding hole 6 and fixed vertically at a predetermined position.

  Since the tapered portion 31 of the container 11 and the magnet tip 3Aa of the magnetic means 3A are in close contact with each other in the fixed state of the container 11, the magnetic fine particles in the solution in the container 11 are magnetized by the magnetic force of the magnetic means 4. The magnetic fine particles are attracted to the means 4 side and can be easily separated.

The magnetic means 4 can be of any type as long as it is composed of a permanent magnet or an electromagnet made of a super strong magnet such as neodymium and generates a magnetic force.
The magnet may be a magnet with multiple poles. The magnet may be covered with a cap made of a magnetic metal such as iron, and the magnet surface may be embedded in the container. The length should be equal to or greater than the diameter of the magnetized surface or the length of one side, and more than twice as long.
The magnetic means 3A is preferably the strongest neodymium magnet in order to quickly collect magnetic fine particles.
Since the magnetic means 3A is a multipole magnetized magnet, a large magnetic field gradient can be generated in the container, so that magnetic fine particles can be collected quickly.
By covering the magnetic means 3A with a cap made of a magnetic metal such as iron, a large magnetic field gradient can be generated in the container 11, so that magnetic fine particles can be collected quickly.
The magnetic means 3A has a stronger magnetic field as it is elongated in the magnetizing direction. When the magnetic means 3A is longer than twice the diameter of the magnetized surface or the length of one side, there is almost no difference in the speed with which the magnetic fine particles gather. .

Further, instead of the plunger 12, a structure provided with a ring formed of a rubber material around the upper portion of the holding hole 6 may be used.
By providing a ring formed of a rubber material around the holding hole 6, the container 11 inserted into the holding hole 6 is held.

Moreover, since the container 11 and the metal are in close contact with each other by forming the groove 2 in accordance with the shape of the container 11, the solution in the container 11 can be well cooled when a magnetic stand is placed on ice.
In order to cool the solution in the container, the material of the base 1 is preferably a metal having a high thermal conductivity, and aluminum, copper, or an alloy thereof having a high thermal conductivity is particularly preferable among the metals.

Next, an embodiment of the magnetic stand 30 according to the present invention will be described. When the base 1 is made of an aluminum alloy and the magnet means 3A is a single-pole magnet having a length 2.4 times the diameter of the magnetized surface, the magnetic stand 30 is placed on ice and the water in the container 11 is placed. When the temperature of was measured, it was cooled to 2 ° C. in 10 minutes.
As a comparison, measurement was carried out using a commercially available resin magnetic stand having a gap between the container and the groove, and it did not fall below 10 ° C. even after 1 hour.
Further, when the speed at which the magnetic particles were collected by these two magnetic stands 30 was examined, the magnetic stand 30 of FIGS. 1 to 3 of the present invention was able to collect 1.5 times faster than the commercially available one. .

The gist of the magnetic stand 30 is as follows.
That is, a base 1 made of metal having a plurality of holding holes 6 for inserting the container 11 and a magnet made of a permanent magnet or an electromagnet provided on the base 1 corresponding to the lower part of the container 11. Means 3A, a positioning recess 51 provided in the lower part of the base 1 for positioning the lower part of the container 11, and a plunger hole 25 provided in the base 1 and positioned in the upper stage of the magnetic means 3A. The plunger 12 provided in the plunger hole 25 for biasing the side of the container 11, the magnet tip 3 Aa of the magnetic means 3 A is in close contact with the taper 31 of the container 11, The magnetic means 3A is magnetized in the longitudinal direction.

  The magnetic stand according to the present invention can collect and recover the magnetic particles in the solution in the container faster and more efficiently than before because the magnet tip of the magnet means can be in close contact with the tapered portion of the lower part of the container. Can do.

DESCRIPTION OF SYMBOLS 1 Base 2 Groove 2a Inner surface 3 Magnet embedding groove 3A Magnetic means 3Aa Magnet tip 6 Holding hole 11 Container 11a Tip 12 Plunger 12a Tip 20 Upper plate portion 21 Lower plate portion 22 Concave portion 22a Back surface 23 Axial direction 24 Hole direction 25 hole for plunger 26 hole for magnet means 30 magnetic stand 31 taper part 50 upper surface 51 concave part for positioning A longitudinal direction

Claims (1)

A holding base (6) for inserting the container (11) and a base (1) made of metal and a permanent base located on the base (1) and corresponding to the lower part of the container (11) Magnetic means (3A) comprising a magnet or an electromagnet, and a positioning recess (51) for positioning the lower part of the container (11) provided at the lower part of the base (1),
The magnetic stand (3A) of the magnetic means (3A) is in close contact with the tapered portion (31) of the container (11), and the magnetic means (3A) is magnetized in the longitudinal direction. In
A plunger hole (25) provided in the base (1) and positioned in the upper stage of the magnetic means (3A), and a side portion of the container (11) provided in the plunger hole (25) A magnetic stand provided with a plunger (12) for carrying out the operation.

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