JP5958249B2 - Inspection chip and inspection device - Google Patents

Description

  The present invention relates to an inspection chip for performing a chemical, medical, or biological inspection of an inspection object.

  Conventionally, an inspection chip for inspecting a biological substance, a chemical substance, or the like is known. For example, the microchip disclosed in Patent Document 1 is an inspection chip having a plurality of sections corresponding to each item in order to perform inspection and analysis of a plurality of items. The sample introduced from the sample introduction port is distributed to each section. The distributed specimen is quantified by the specimen weighing section of each section. The sample quantified by each sample measuring unit is introduced into the detection unit of each section and used for optical measurement of each item.

JP 2009-109429 A

  The microchip disclosed in Patent Document 1 has a thin plate shape, and a fluid circuit including a plurality of sections is formed on a substrate surface. In this microchip, since it is necessary to provide a plurality of detection units at different positions on the substrate surface, the area of the substrate surface increases as the number of detection units increases. As the area of the substrate surface increases, the total length of the liquid flow path formed on the substrate surface tends to increase. Therefore, the rotation speed of the inspection chip and the application time of centrifugal force at the time of inspection may increase.

  The objective of this invention is providing the test | inspection chip and test | inspection apparatus which can test | inspect a some target object, suppressing the full length of a liquid flow path.

  In the first aspect of the present invention, a liquid specimen and reagent are injected, a centrifugal force is applied by rotating about a predetermined first axis, and a second axis different from the first axis is provided. A test chip in which the direction of the centrifugal force is changed by being rotated to the center, and can store the mixed liquid of the sample and the reagent that flows in, and the stored mixed liquid is measured Two reservoirs which are parts to be performed, and two quantification units including a space capable of quantifying the amount of the specimen or the reagent respectively flowing into the two reservoirs, the two reservoirs are A first reservoir provided on one of the front surface and the back surface of the inspection chip, and a second reservoir provided on the other of the front surface and the back surface, wherein the two quantification units determine the amount of the liquid. Of the specimen or the reagent when A first quantification unit and a second quantification unit each having two quantification ends on which a surface is formed, wherein the test chip is rotated about the first axis, and the front surface and the back surface are opposed to each other. In a state where the front and back direction and the axial direction of the second axis are parallel, at least one of the two quantitative end portions provided in the first quantitative unit, and the two provided in the second quantitative unit At least one of the fixed end portions is located at an equal distance from the first axis.

  According to the test chip according to the above aspect, two reservoirs each storing and measuring a mixed solution of the specimen and the reagent, and two liquids capable of quantifying the amount of the specimen or the reagent respectively flowing into the two reservoirs And a quantitative unit. A first reservoir and a second reservoir are provided on the front and back surfaces of the inspection chip. The first quantification unit and the second quantification unit include two quantification end portions on which a liquid surface is formed during quantification. Since the two storage portions are arranged on the front and back surfaces of the inspection chip, a plurality of mixed liquids can be inspected while suppressing the total length of the liquid flow path. Furthermore, in the state where the test chip is rotated around the first axis and the front and back directions and the axial direction of the second axis are parallel, the quantitative amount of the second quantitative part and at least one of the quantitative end parts of the first quantitative part At least one of the end portions is located at an equal distance from the first axis. Accordingly, the centrifugal force applied to the two quantification units becomes equal, and the reagent or specimen can be accurately quantified.

  The two quantification units may include a wall surface that forms a recess capable of storing the specimen or the reagent, and the inclination angles of the wall surfaces may be equal when projected in the front-back direction. In this case, it is possible to increase the accuracy of quantification in the quantification unit while suppressing the total length of the liquid channel.

  You may provide the common surplus part which can connect the said two fixed_quantity | quantitative_assay part and can accommodate the said test substance or the reagent flowed out from each said fixed_quantity | quantitative_assay part. In this case, it is possible to reduce the possibility that the specimen or reagent overflows from the surplus portion while suppressing the overall length of the liquid flow path. Therefore, it is possible to reduce the possibility that the overflowing specimen or reagent is mixed into the solution to be measured and the inspection accuracy is lowered.

  The first storage unit and the first quantification unit are included, the sample and the reagent are movable, the first flow path is movable, the second storage unit and the second quantification unit are included, and the sample and the reagent are moved A second flow path that is possible, and the first flow path and the second flow path may have a shape that matches when projected in the front-back direction. In this case, the reagent and the specimen can move in the same way in the first channel and the second channel. As a result, two mixed solutions can be inspected at the same time while suppressing the total length of the liquid flow path, and the inspection time can be shortened.

  The first storage unit and the first quantification unit are included, the sample and the reagent are movable, the first flow path is movable, the second storage unit and the second quantification unit are included, and the sample and the reagent are moved A second flow path that is possible, wherein the second flow path is downstream of the specimen or the reagent in the movement direction with respect to the second quantification section, and the specimen or the second storage section You may provide the standby part provided in the upstream of the movement direction of the said reagent which can store the said liquid mixture. In this case, by providing the standby portion in the second flow path, the timing at which the liquid mixture is stored in each storage section in the first flow path and the second flow path can be shifted. As a result, the two liquid mixtures can be inspected separately by the common measurement light. Since the mutual measurement does not interfere even with common measurement light while suppressing the overall length of the liquid flow path, the inspection accuracy can be increased.

  One substrate that forms the front surface and the back surface may be provided, the first flow path may be formed on the front surface of the substrate, and the second flow path may be formed on the back surface of the substrate. In this case, the inspection chip in which the first flow path and the second flow path are formed on both surfaces can be manufactured using one mold. As a result, the manufacturing cost of the inspection chip in which the total length of the liquid channel is suppressed can be reduced.

  A first inlet capable of injecting the specimen or the reagent into the first flow path; and a second inlet capable of injecting the specimen or the reagent into the second flow path; The second inlet may be formed on different surfaces of the inspection chip. In this case, it is possible to prevent a reagent or specimen from being misplaced while suppressing the total length of the liquid channel.

A second aspect of the present invention is an inspection apparatus capable of using an inspection chip into which a specimen and a reagent that are liquids are injected, wherein the inspection chip can move the specimen and the reagent, and the inspection chip A first channel provided on one of the front surface and the back surface, and a second channel provided on the other of the front surface and the back surface, the sample and the reagent being movable, Comprises a first reservoir that can store a mixed solution of the specimen and the reagent injected into the first flow path, and the second flow path includes the sample and the reagent injected into the second flow path A second storage unit capable of storing the mixed liquid, and the inspection apparatus emits light to the object and can perform optical measurement, and a predetermined first axis and the first axis are different from each other . A rotating part capable of rotating the inspection chip around two axes Rotating the test chip by the rotating unit generates a first mixed liquid that is the mixed liquid of the specimen and the reagent that moves in the first flow path, and moves in the second flow path. A liquid mixing unit that generates a second mixed liquid that is the mixed liquid of the specimen and the reagent, and the first storage unit when the first mixed liquid and the second mixed liquid are generated by the liquid mixing unit. Measurement control means for optically measuring the first mixed liquid stored in the second storage liquid and the second mixed liquid stored in the second storage section simultaneously by the measurement section, the measurement control means comprising: The different components are measured from the first mixed solution and the second mixed solution based on the wavelength of the light.

  According to the inspection apparatus according to the above aspect, since the specimen and reagent flow paths can be inspected using the inspection chips arranged on the front surface and the back surface, a plurality of liquid flow paths provided in the inspection chip are suppressed while Can be inspected. Further, different components are measured based on the wavelength of light from the first mixed liquid stored in the first storage section and the second mixed liquid stored in the second storage section. Therefore, the first mixed liquid and the second mixed liquid can be measured simultaneously with the common measurement light. As a result, the two mixed liquids can be inspected at the same time while suppressing the total length of the liquid flow path, so that the inspection time can be shortened.

It is a rear view of the inspection apparatus 1 in which the inspection chip 2 is in a steady state. It is the other rear view of the inspection apparatus 1 which the test | inspection chip 2 rotated from the steady state. It is a top view of the inspection apparatus 1 shown in FIG. 3 is a block diagram showing an electrical configuration of a control device 90. FIG. 3 is a block diagram showing an electrical configuration of a measurement unit 7. FIG. It is a perspective view of the test | inspection chip 2 which concerns on 1st embodiment. It is a front view of the test | inspection chip 2 before the centrifugation process based on 1st embodiment. It is a rear view of the test | inspection chip 2 before the centrifugation process based on 1st embodiment. It is a flowchart of the main process of the test | inspection apparatus 1 based on 1st embodiment. It is a front view of the test | inspection chip 2 revolved in the autorotation angle of 90 degree | times after FIG. It is a front view of the test | inspection chip 2 revolved in 90 autorotation angles after FIG. FIG. 12 is an enlarged front view of the lower right portion of the inspection chip 2 revolved at a rotation angle of 0 degree following FIG. 11. FIG. 13 is a front view of the inspection chip 2 revolved at a rotation angle of 90 degrees following FIG. 12. It is a perspective view of the test | inspection chip 2 which concerns on 2nd embodiment. It is a flowchart of the main process of the test | inspection apparatus 1 based on 2nd embodiment. It is a rear view of the test | inspection chip 2 revolved in the autorotation angle 0 degree | times based on 2nd embodiment. FIG. 17 is a rear view of the inspection chip 2 optically measured at a rotation angle of 0 degree following FIG. 16. It is a rear view of the test | inspection chip 2 optically measured after the rotation angle of 0 degree | times after FIG.

  Embodiments of the present invention will be described with reference to the drawings. The drawings to be referred to are used to explain technical features that can be adopted by the present invention, and are merely illustrative examples.

<1. First embodiment>
An embodiment of the present invention will be described. A schematic structure of the inspection system 3 will be described with reference to FIGS. 1 and 2. The inspection system 3 of the present embodiment includes an inspection chip 2 that can store a specimen and a reagent that are liquids, and an inspection apparatus 1 that performs an inspection using the inspection chip 2. The inspection apparatus 1 can apply a centrifugal force to the inspection chip 2 by rotation about a vertical axis separated from the inspection chip 2. The inspection apparatus 1 can switch the centrifugal direction that is the direction of the centrifugal force applied to the inspection chip 2 by rotating the inspection chip 2 about the horizontal axis.

<1-1. Detailed structure of the inspection apparatus 1>
With reference to FIGS. 1-4, the detailed structure of the inspection apparatus 1 is demonstrated. In the following description, the upper, lower, right, left, front side, and rear side of FIG. 1 and FIG. 2 are respectively the upper, lower, right, left, rear, and rear sides of the inspection apparatus 1. Let it be in front. The upper, lower, right, left, front side, and back side of FIG. 3 are the front, lower, right, left, upper, and lower sides of the inspection apparatus 1, respectively. In this embodiment, the direction of the vertical axis is the vertical direction, and the direction of the horizontal axis is the direction of the speed when the inspection chip 2 rotates around the vertical axis. For ease of understanding, FIGS. 1 and 2 show the upper housing 30 by phantom lines, and FIG. 3 shows a state where the top plate of the upper housing 30 is removed.

  As shown in FIGS. 1 to 3, the inspection apparatus 1 includes an upper housing 30, a lower housing 31, a turntable 33, an angle changing mechanism 34, and a control device 90. The turntable 33 is a disk-shaped rotating body provided on the upper surface side of the lower housing 31. The inspection chip 2 is held above the turntable 33. The angle changing mechanism 34 is a drive mechanism provided on the turntable 33. This drive mechanism rotates the inspection chip 2 around the horizontal axis. The upper housing 30 is fixed to the upper side of the lower housing 31, and the measurement unit 7 that performs optical measurement on the inspection chip 2 is provided inside. The control device 90 is a controller that controls various processes of the inspection device 1.

  The detailed structure of the lower housing 31 will be described. As shown in FIGS. 1 and 2, the lower housing 31 has a box-like frame structure in which frame members are combined. An upper plate 32 that is a rectangular plate material is provided on the upper surface of the lower housing 31. A turntable 33 is rotatably provided above the upper plate 32. A drive mechanism for rotating the turntable 33 around the vertical axis is provided in the lower housing 31 as follows.

  A spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side in the lower housing 31. A shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed. A vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31. The main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31. The upper end portion of the main shaft 57 is connected to the center portion of the turntable 33.

  The main shaft 57 is rotatably held by a support member 53 provided immediately below the upper plate 32. A pulley 38 is fixed to the main shaft 57 below the support member 53. A belt 39 is stretched over the pulleys 37 and 38. When the main shaft motor 35 rotates the shaft 36, driving force is transmitted to the main shaft 57 via the pulley 37, the belt 39, and the pulley 38. At this time, the turntable 33 rotates around the main shaft 57 in conjunction with the rotation of the main shaft 57.

  A guide rail 56 extending in the vertical direction inside the lower housing 31 is provided on the right side in the lower housing 31. The T-shaped plate 48 is movable in the vertical direction in the lower housing 31 along the guide rail 56. A groove 80 that is long in the left-right direction is formed on the front side of the T-shaped plate 48, that is, on the back side in FIG. 1 and FIG.

  The aforementioned main shaft 57 is a cylindrical body having a hollow inside. The inner shaft 40 is a shaft that can move in the vertical direction inside the main shaft 57. An upper end portion of the inner shaft 40 passes through the main shaft 57 and is connected to a rack gear 43 described later. A bearing 41 is provided at the left end of the T-shaped plate 48. Inside the bearing 41, the lower end portion of the inner shaft 40 is rotatably held.

  A stepping motor 51 for moving the T-shaped plate 48 up and down is fixed in front of the T-shaped plate 48. The shaft 58 of the stepping motor 51 protrudes rearward, that is, toward the front side of the page in FIGS. A disc-shaped cam plate 59 is fixed to the tip of the shaft 58. A cylindrical projection 70 is provided on the rear surface of the cam plate 59. The tip of the protrusion 70 is inserted into the groove 80 described above. The protrusion 70 can slide in the groove 80. When the stepping motor 51 rotates the shaft 58, the projection 70 moves up and down in conjunction with the rotation of the cam plate 59. At this time, the T-shaped plate 48 moves up and down along the guide rail 56 in conjunction with the protrusion 70 inserted in the groove 80.

  The detailed structure of the angle changing mechanism 34 will be described. The angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33. A rack gear 43 fixed to the inner shaft 40 is provided between the pair of L-shaped plates 60. The rack gear 43 is a metal plate-like member that is long in the vertical direction, and gears are respectively carved on both end faces.

  On the front end side in the extending direction of each L-shaped plate 60, a horizontal support shaft 46 having a gear 45 is rotatably supported. The support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown). For this reason, the inspection chip 2 also rotates around the support shaft 46 in conjunction with the rotation of the gear 45. Between the gear 45 and the rack gear 43, a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis is interposed. The pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 rotates about the support shaft 46.

  In the present embodiment, as the main shaft motor 35 rotates and drives the turntable 33, the inspection chip 2 rotates around the main shaft 57, which is a vertical axis, and centrifugal force is applied to the inspection chip 2. The rotation around the vertical axis of the inspection chip 2 is called revolution. On the other hand, as the stepping motor 51 moves the inner shaft 40 up and down, the inspection chip 2 rotates around the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively. . The rotation around the horizontal axis of the inspection chip 2 is called rotation.

  As shown in FIG. 1, when the T-shaped plate 48 is lowered to the lowermost end of the movable range, the rack gear 43 is also lowered to the lowermost end of the movable range. At this time, the inspection chip 2 is in a steady state where the rotation angle is 0 degree. As shown in FIG. 2, when the T-shaped plate 48 is raised to the uppermost end of the movable range, the rack gear 43 is also raised to the uppermost end of the movable range. At this time, the test | inspection chip 2 will be in the state rotated 180 degree | times centering on the horizontal axis line from a steady state. That is, in this embodiment, the angle width that the test chip 2 can rotate is the rotation angle of 0 degrees to 180 degrees.

  The detailed structure of the upper housing 30 will be described. As shown in FIG. 3, the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.

  The measurement unit 7 provided in the upper housing 30 includes a light source 71 that emits measurement light and a sensor unit 72 that detects the measurement light emitted from the light source 71. The light source 71 and the sensor unit 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2. In the present embodiment, the position on the left side of the main shaft 57 in the reciprocable range of the inspection chip 2 is the measurement position at which the inspection chip 2 is irradiated with the measurement light. When the inspection chip 2 is at the measurement position, the measurement light connecting the light source 71 and the sensor unit 72 intersects the front and rear surfaces of the inspection chip 2 perpendicularly.

  The electrical configuration of the control device 90 will be described with reference to FIG. The control device 90 includes a CPU 91 that performs main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for a user to input instructions to the control device 90, a hard disk device 95 for storing various data and programs, and a display 96 for displaying various information.

  Further, a revolution controller 97, a rotation controller 98, and a measurement controller 99 are connected to the CPU 91. The revolution controller 97 controls the revolution of the inspection chip 2 by transmitting a control signal for rotating the spindle motor 35 to the spindle motor 35. The rotation controller 98 controls the rotation of the inspection chip 2 by transmitting a control signal for rotating the stepping motor 51 to the stepping motor 51. The measurement controller 99 performs optical measurement of the inspection chip 2 by driving the measurement unit 7. Specifically, the measurement controller 99 transmits a control signal for executing light emission of the light source 71 and light detection of the sensor unit 72 to the light source 71 and the sensor unit 72.

  With reference to FIG. 5, the detailed structure of the measurement part 7 is demonstrated. In the present embodiment, the sensor unit 72 includes a half mirror 72A, a band pass filter 72B, a light receiving sensor 72C, a band pass filter 72D, and a light receiving sensor 72E. The light source 71 irradiates halogen light as measurement light toward the half mirror 72A. The halogen light transmitted through the half mirror 72A is applied to the band-pass filter 72B. The band-pass filter 72B can transmit only a light component having a wavelength of 340 nm. The light component transmitted through the band-pass filter 72B is detected by the light receiving sensor 72C. On the other hand, the halogen light reflected by the half mirror 72A is applied to the band-pass filter 72D. The band-pass filter 72D can transmit only a light component having a wavelength of 650 nm. The light component transmitted through the band-pass filter 72D is detected by the light receiving sensor 72E.

<1-2. Structure of inspection chip 2>
With reference to FIGS. 6-8, the detailed structure of the test | inspection chip 2 which concerns on 1st embodiment is demonstrated. In the following description, the upper, lower, lower right, upper left, upper right, and lower left in FIG. 6 are the upper, lower, front, rear, right, and left sides of the test chip 2, respectively. 7 and 8 show front views of the inspection chip 2 with the sheet 29 removed. The same applies to FIGS. 10 to 13, 15, 17, and 18 described later.

  As shown in FIG. 6, the inspection chip 2 has a square shape when viewed from the front as an example, and mainly includes a transparent synthetic resin plate 20 having a predetermined thickness. The front surface 25 of the plate 20 is sealed with a sheet 29 made of a transparent synthetic resin thin plate. Between the surface 25 and the sheet | seat 29, as shown in FIG. 7, the 1st flow path 100 which can move a liquid is formed. The first flow path 100 is a recess formed in the surface 25 of the plate member 20 with a predetermined depth, and extends in a direction orthogonal to the thickness direction of the plate member 20.

  Similarly, the back surface 26 which is the rear surface of the plate member 20 is also sealed with a sheet (not shown). Between the back surface 26 and the sheet, as shown in FIG. 8, a second flow path 200 in which the liquid can move is formed. The second flow path 200 is a recess formed in the back surface 26 of the plate member 20 with a predetermined depth, and extends in a direction orthogonal to the thickness direction of the plate member 20. Thus, the 1st flow path 100 and the 2nd flow path 200 which were formed in the front and back of the test | inspection chip 2 make a mirror image on both sides of the front-back direction center of the board | plate material 20. As shown in FIG. In other words, the first flow path 100 and the second flow path 200 have shapes that match when the front surface 25 and the back surface 26 are projected in the front-rear direction facing each other.

  With reference to FIG. 7, the detailed structure of the 1st flow path 100 is demonstrated. The first channel 100 includes a sample injection unit 111, a sample determination unit 114, a sample surplus unit 116, a reagent injection unit 131, a reagent determination unit 134, a reagent surplus unit 136, a reagent injection unit 151, a reagent determination unit 154, and a reagent surplus unit 156. , Standby unit 171, and first storage unit 172. The specimen injection part 111 is a part into which the specimen 10 is injected and stored, and is a recess that opens upward. The reagent injection part 131 is a part where the first reagent 12 is injected and stored, and is a concave part opened upward. The reagent injection part 151 is a part into which the second reagent 14 is injected and stored, and is a recess that opens upward. As an example, the specimen 10 of the present embodiment is blood, and the first reagent 12 and the second reagent 14 are a reagent for measuring glucose in blood, or blood, or a diluted solution for diluting the reagent.

  The specimen injection part 111, the reagent injection part 131, and the reagent injection part 151 are formed side by side on the surface 25 along the upper side part 21 that is the upper wall surface of the test chip 2. Of the sample injection unit 111, the reagent injection unit 131, and the reagent injection unit 151, the sample injection unit 111 is closest to the left side portion 23 that is the left wall surface of the test chip 2. The reagent injection part 151 is closest to the right side part 22 which is the right wall surface of the test chip 2. The reagent injection part 131 is located between the sample injection part 111 and the reagent injection part 151. The lower wall surface of the inspection chip 2 is the lower side portion 24.

  Although not shown, an injection hole for injecting the sample 10 into the sample injection unit 111 is formed in the sheet 29 that seals the surface 25. For example, the specimen 10 accommodated in an instrument (not shown) may be injected from the injection hole by a user operation. That is, the sample 10 may be injected into the sample injection unit 111 through the injection hole using a known method. Similarly, in the sheet 29 that seals the surface 25, although not shown, an injection hole for injecting the first reagent 12 into the reagent injection part 131 and an injection for injecting the second reagent 14 into the reagent injection part 151 A hole is formed. These injection holes may have a shape in which, for example, the upper side portion 21 is open.

  The sample supply path 112 is a flow path of the sample 10 that extends downward from the upper right part of the sample injection unit 111. The lower end portion of the sample supply path 112 is connected to a sample supply section 113 having a narrow flow path. A sample quantitative unit 114 is provided below the sample supply unit 113. The specimen quantification unit 114 is a part that quantifies the specimen 10, and is a recess that opens upward. The sample quantification unit 114 includes two quantification end portions 114A and 114B in which the liquid level of the sample 10 is formed when the liquid amount is quantified. By applying a centrifugal force from the sample supply unit 113 toward the inside of the recess of the sample quantification unit 114, the same amount of the sample 10 as the volume inside the recess of the sample quantification unit 114 is quantified.

  The first passage 115 extends leftward and the second passage 117 extends rightward from the part where the sample supply unit 113 and the sample determination unit 114 communicate with each other. The first passage 115 extends to the sample surplus part 116 provided below the sample determination unit 114. That is, the first passage 115 is bent when viewed from the front so that the flow path forming direction changes. Thereby, in order for the sample 10 to move between the sample quantification unit 114 and the sample surplus unit 116 in the first passage 115, it is necessary to apply external forces in a plurality of different directions to the sample 10. That is, the specimen 10 stored in the specimen surplus section 116 is unlikely to flow back to the specimen quantitative section 114 via the first passage 115.

  The specimen surplus part 116 is a part where the specimen 10 overflowing from the specimen quantification part 114 is stored, and is a concave part extending rightward from the lower end part of the first passage 115. The second passage 117 extends downward through the right side of the sample determination unit 114 and is connected to the upper left end of the standby unit 171 described later. That is, the flow path formation direction of the second passage 117 changes for the same reason as the first passage 115.

  The reagent supply path 132 is a flow path of the first reagent 12 that extends downward from the upper right portion of the reagent injection part 131. The lower end of the reagent supply path 132 is connected to a reagent supply part 133 having a narrow channel. A reagent quantitative unit 134 is provided below the reagent supply unit 133. The reagent quantification unit 134 is a part that quantifies the first reagent 12 and is a concave portion that opens upward. The reagent quantification unit 134 includes two quantification end portions 134A and 134B that form the liquid surface of the first reagent 12 when the amount of the liquid is quantified. By applying a centrifugal force from the reagent supply unit 133 toward the inside of the recess of the reagent quantification unit 134, the same amount of the first reagent 12 as the volume inside the recess of the reagent quantification unit 134 is quantified.

  The third passage 135 extends leftward and the fourth passage 137 extends rightward from the portion where the reagent supply unit 133 and the reagent quantitative unit 134 communicate. The third passage 135 extends to the reagent surplus portion 136 provided below the reagent fixed amount portion 134. That is, the flow path formation direction of the third passage 135 changes for the same reason as the first passage 115. The reagent surplus portion 136 is a portion in which the first reagent 12 overflowing from the reagent quantitative portion 134 is stored, and is a concave portion extending rightward from the lower end portion of the third passage 135. The fourth passage 137 extends downward through the right side of the reagent quantification unit 134 and is connected to a central upper end of a standby unit 171 described later. In other words, the flow path formation direction of the fourth passage 137 changes for the same reason as the first passage 115.

  The reagent supply path 152 is a flow path for the second reagent 14 that extends downward from the upper right portion of the reagent injection portion 151. The lower end portion of the reagent supply path 152 is connected to a reagent supply section 153 having a narrow flow path. A reagent quantitative unit 154 is provided below the reagent supply unit 153. The reagent quantification unit 154 is a part that quantifies the second reagent 14 and is a recess that opens upward. The reagent quantification unit 154 includes two quantification end portions 154A and 154B on which the liquid surface of the second reagent 14 is formed when the liquid amount is quantified. By applying a centrifugal force from the reagent supply unit 153 toward the inside of the recess of the reagent quantification unit 154, the second reagent 14 having the same volume as the volume inside the recess of the reagent quantification unit 154 is quantified.

  A fifth passage 155 extends leftward and a sixth passage 157 extends rightward from a portion where the reagent supply unit 153 and the reagent quantitative unit 154 communicate with each other. The fifth passage 155 extends to the reagent surplus portion 156 provided below the reagent fixed amount portion 154. That is, the flow path formation direction of the fifth passage 155 changes for the same reason as the first passage 115. The reagent surplus portion 156 is a portion in which the second reagent 14 overflowing from the reagent quantitative portion 154 is stored, and is a concave portion extending rightward from the lower end portion of the fifth passage 155. The sixth passage 157 extends downward through the right side of the reagent quantitative unit 154 and is connected to the upper right end of a first storage unit 172 described later. That is, in the sixth passage 157, the flow path formation direction changes for the same reason as the first passage 115.

  The standby unit 171 is a trapezoidal concave portion that is provided at the lower center of the surface 25 and opens upward. In the standby unit 171, the sample 10 flowing from the second passage 117 and the first reagent 12 flowing from the fourth passage 137 are stored, and the intermediate solution 16 in which the sample 10 and the first reagent 12 shown in FIG. Is generated. The first reservoir 172 is a rectangular recess provided in the lower right portion of the surface 25 and opening upward. The first reservoir 172 is a part where the sample 10 flowing from the second passage 117 and the intermediate liquid 16 generated in the standby unit 171 are mixed to generate the first mixed liquid 18 shown in FIG. The generated intermediate liquid 16 is optically measured by measurement light passing through the first reservoir 172, as will be described later.

  An isolation wall 173 extends between the standby unit 171 and the first storage unit 172. The isolation wall 173 forms a right wall surface of the standby unit 171 and forms a left wall surface of the first storage unit 172. The standby part 171 and the first storage part 172 communicate with each other through a gap formed between the isolation wall 173 and the wall part provided below the reagent surplus part 156. That is, the upper right part of the standby part 171 and the upper left part of the first storage part 172 are connected.

  As shown in FIG. 12, a stirring unit 180 for efficiently generating the first mixed liquid 18 shown in FIG. 13 is provided in the upper right part of the first storage unit 172. In the stirring unit 180, three projecting portions 181 to 183 projecting leftward from the right wall surface of the first storage unit 172 are provided side by side in the vertical direction.

  A virtual line α shown in FIG. 12 extends rightward from the upper end of the isolation wall 173. The position h1 is a position where the line α intersects with the right wall surface 172A of the first reservoir 172. A virtual line β shown in FIG. 12 extends from the upper end portion 173A of the isolation wall 173 in the lower right direction and forms 90 degrees with the left wall surface 171A of the standby portion 171 when viewed from the front. The position h2 is a position where the line β intersects with the right wall surface 172A of the first reservoir 172. The position, shape, and size of the stirring unit 180 are defined as follows.

  It is preferable that the protruding portion of the stirring unit 180 is provided in a range from the position h1 to the position h2. In the present embodiment, the protrusions 182 and 183 are provided in the range from the position h1 to the position h2.

  It is preferable that the protruding ends 181A to 183A of the protruding portions 181 to 183 of the stirring unit 180 are located on the extension of the line α or the line β. In the present embodiment, the protruding end 182A of the protruding portion 182 is located on the extension of the line α. The protruding end 183A of the protruding portion 183 is located on the extension of the line β.

  The protruding ends 181A to 183A of the protruding portions 181 to 183 of the stirring unit 180 are preferably higher than the liquid level of the liquid stored in the first storage portion 172. In the present embodiment, the second reagent 14 is stored along the right wall surface 172A of the first storage unit 172 in the process of the centrifugal processing described later. Although details will be described later, at this time, the projecting ends 181A to 183A of the three projecting portions 181 to 183 are exposed to the outside of the liquid surface of the second reagent 14.

  It is preferable that the wall surfaces of the protrusions 181 to 183 included in the stirring unit 180 are inclined with respect to the direction of the centrifugal force X and the direction of the gravity Z. In this embodiment, as for all the three protrusion parts 181 to 183, the upper wall surfaces 181B to 183B extend from the respective protrusion ends 181A to 183A in the upper right direction. Lower wall surfaces 181C to 183C extend from the respective projecting ends 181A to 183A in the lower right direction. Therefore, the wall surfaces of the three projecting portions 181 to 183 are all inclined with respect to the right direction where the centrifugal force X shown in FIG. 12 acts and the downward direction where the gravity Z shown in FIG. 7 acts.

  With reference to FIG. 8, the detailed structure of the 2nd flow path 200 is demonstrated. Similar to the first flow path 100, the second flow path 200 includes a sample injection section 211, a sample determination section 214, a sample surplus section 216, a reagent injection section 231, a reagent determination section 234, a reagent surplus section 236, a reagent injection section 251, A reagent quantification unit 254, a reagent surplus unit 256, a standby unit 271 and a second storage unit 272 are included. As described above, the second flow path 200 has a shape that forms a mirror image with respect to the first flow path 100. Therefore, the external appearance of the first flow channel 100 viewed from the front as shown in FIG. 7 and the first flow channel 100 viewed from the rear as shown in FIG. 8 are reversed in the left-right direction.

  The sample injection part 211, the reagent injection part 231, and the reagent injection part 251 are parts where the sample 11, the first reagent 13, and the second reagent 15 are injected and stored, respectively. As an example, the specimen 11 of this embodiment is blood, and the first reagent 13 and the second reagent 15 are a reagent for measuring total cholesterol in blood, blood, or a diluted solution for diluting the reagent. The specimen injection part 211, the reagent injection part 231, and the reagent injection part 251 are formed side by side on the back surface 26 along the upper side part 21 in the left-right direction. Although not shown, an injection hole for injecting the specimen 11, the first reagent 13, and the second reagent 15 is formed in the sheet that seals the back surface 26. Note that the sample 10 and the sample 11 may be the same sample.

  The sample supply path 212 is a flow path of the sample 11 that extends downward from the upper right portion of the sample injection unit 211. The lower end portion of the sample supply path 212 is connected to a sample supply section 213 having a narrow flow path. Below the sample supply unit 213, a sample quantification unit 214 that quantifies the sample 11 is provided. The sample quantification unit 214 includes two quantification end portions 214A and 214B that form the liquid surface of the sample 11 when the amount of the liquid is quantified. A first passage 215 extends leftward and a second passage 217 extends rightward from a portion where the sample supply unit 213 and the sample determination unit 214 communicate with each other. The first passage 215 extends to the sample surplus part 216 provided below the sample determination unit 214. The specimen surplus part 216 is a part where the specimen 11 overflowing from the specimen quantification part 214 is stored, and is a concave part extending rightward from the lower end part of the first passage 215. The second passage 217 extends downward through the right side of the sample determination unit 214 and is connected to the upper left end of the standby unit 271 described later.

  The reagent supply path 232 is a flow path of the first reagent 13 that extends downward from the upper right portion of the reagent injection part 231. The lower end part of the reagent supply path 232 is connected to a reagent supply part 233 having a narrow channel. A reagent quantification unit 234 that quantifies the first reagent 13 is provided below the reagent supply unit 233. The reagent quantification unit 234 includes two quantification end portions 234A and 234B on which the liquid surface of the first reagent 13 is formed when the liquid amount is quantified. A third passage 235 extends leftward and a fourth passage 237 extends rightward from a portion where the reagent supply unit 233 and the reagent quantitative unit 234 communicate with each other. The third passage 235 extends to the reagent surplus portion 236 provided below the reagent fixed amount portion 234. The reagent surplus portion 236 is a portion in which the first reagent 13 overflowing from the reagent quantitative portion 234 is stored, and is a concave portion extending rightward from the lower end portion of the third passage 235. The fourth passage 237 extends downward through the right side of the reagent quantitative unit 234 and is connected to a central upper end of a standby unit 271 described later.

  The reagent supply path 252 is a flow path of the second reagent 15 that extends downward from the upper right portion of the reagent injection part 251. The lower end of the reagent supply path 252 is connected to a reagent supply section 253 having a narrow flow path. Below the reagent supply unit 253, a reagent quantification unit 254 that quantifies the second reagent 15 is provided. The reagent quantification unit 254 includes two quantification end portions 254A and 254B on which the liquid surface of the second reagent 15 is formed when the liquid amount is quantified. The fifth passage 255 extends leftward and the sixth passage 257 extends rightward from the portion where the reagent supply unit 253 and the reagent quantitative unit 254 communicate with each other. The fifth passage 255 extends to the reagent surplus portion 256 provided below the reagent fixed amount portion 254. The reagent surplus portion 256 is a portion in which the second reagent 15 overflowing from the reagent quantitative portion 254 is stored, and is a concave portion extending rightward from the lower end portion of the fifth passage 255. The sixth passage 257 extends downward through the right side of the reagent quantitative unit 254 and is connected to the upper right end of the second storage unit 272 described later.

  The standby unit 271 is a trapezoidal concave portion provided in the lower center of the back surface 26 and opening upward. In the standby unit 271, the sample 11 flowing from the second passage 217 and the first reagent 13 flowing from the fourth passage 237 are stored, and the intermediate solution 17 in which the sample 11 and the first reagent 13 shown in FIG. Is generated. The second reservoir 272 is a rectangular recess provided in the lower right portion of the back surface 26 and opening upward. In the second reservoir 272, the specimen 11 flowing in from the second passage 217 and the intermediate liquid 17 generated in the standby unit 271 are mixed to generate the second mixed liquid 19 shown in FIG. The generated second mixed liquid 19 is optically measured by measurement light passing through the second storage portion 272, as will be described later. Furthermore, a stirring unit 280 for efficiently generating the second mixed liquid 19 is provided on the left wall surface of the second storage unit 272.

  An isolation wall 273 extends between the standby unit 271 and the second storage unit 272. The isolation wall 273 forms a left wall surface of the standby unit 271 and forms a right wall surface of the second storage unit 272. The standby part 271 and the second storage part 272 communicate with each other through a gap formed between the isolation wall 273 and the wall part provided below the reagent surplus part 256. That is, the upper right part of the standby part 271 and the upper left part of the second storage part 272 are connected.

<1-3. Other structures of inspection chip 2>
As shown in FIG. 1, the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 20 via a mounting holder (not shown). As the support shaft 46 rotates, the inspection chip 2 rotates around the support shaft 46. When the inspection chip 2 is in the steady state shown in FIGS. 6 to 8, the upper side 21 and the lower side 24 are orthogonal to the direction of the gravity Z, the right side 22 and the left side 23 are parallel to the direction of the gravity Z, and The left side portion 23 is disposed closer to the main shaft 57 than the right side portion 22. When the inspection chip 2 in the steady state is arranged at the measurement position, the measurement light connecting the light source 71 and the sensor unit 72 passes vertically through the first storage unit 172 and the second storage unit 272 as viewed from above.

<1-4. Example of inspection method>
An inspection method using the inspection apparatus 1 and the inspection chip 2 according to the first embodiment will be described with reference to FIGS. The user attaches the inspection chip 2 to the spindle 46 and inputs a process start command from the operation unit 94. As a result, the CPU 91 shown in FIG. 4 executes the main process shown in FIG. 9 based on the control program stored in the ROM 93. The inspection apparatus 1 can inspect two inspection chips 2 at the same time. However, for convenience of explanation, one inspection chip 2 located outside the upper housing 30 shown in FIGS. The procedure for inspection will be described.

  As shown in FIG. 9, the production | generation of the 1st liquid mixture 18 and the 2nd liquid mixture 19 is first performed with the following procedures (S1). In addition, FIGS. 11-13 has shown the liquid in the 2nd flow path 200 provided in the back surface 26 with the virtual line together with the 1st flow path 100 provided in the surface 25 of the test | inspection chip 2. FIG.

  In step S1, first, the revolution controller 97 shown in FIG. 4 controls the spindle motor 35, whereby the stationary inspection chip 2 shown in FIGS. 6 to 8 is revolved. A centrifugal force X acts on the inspection chip 2 toward the downstream side in the centrifugal direction. In the present embodiment, the inspection chip 2 is set in the inspection apparatus 1 so that the right direction shown in FIG. 7 and the left direction shown in FIG. Therefore, when the inspection chip 2 in the steady state is revolved, the centrifugal force X acts from the left side portion 23 toward the right side portion 22.

  Due to the action of the centrifugal force X, the specimen 10 stored in the specimen injection part 111 moves to the specimen supply path 112 in the first flow path 100 shown in FIG. The first reagent 12 stored in the reagent injection part 131 moves to the reagent supply path 132. The second reagent 14 stored in the reagent injection part 151 moves to the reagent supply path 152. At the same time, in the second channel 200 shown in FIG. 8, the sample 11 stored in the sample injection unit 211 moves to the sample supply path 212. The first reagent 13 stored in the reagent injection part 231 moves to the reagent supply path 232. The second reagent 15 stored in the reagent injection part 251 moves to the reagent supply path 252.

  Next, when the rotation controller 98 shown in FIG. 4 controls the stepping motor 51, the test chip 2 in the revolution state is rotated 90 degrees counterclockwise as viewed from the front as shown in FIG. As a result, the rotation angle of the inspection chip 2 changes to 90 degrees, and the centrifugal force X acts from the upper side portion 21 toward the lower side portion 24.

  Due to the action of the centrifugal force X, the sample 10 flows into the sample determination unit 114 via the sample supply unit 113 in the first channel 100. In the sample determination unit 114, the sample 10 exceeding a predetermined amount overflows into the first passage 115 and is stored in the sample surplus unit 116. As a result, the specimen 10 is quantified. Similarly, the first reagent 12 flows into the reagent quantitative unit 134 via the reagent supply unit 133 and is quantified. The surplus first reagent 12 overflowed at the time of quantification in the reagent quantification unit 134 is accommodated in the reagent surplus unit 136 via the third passage 135. The second reagent 14 flows into the reagent quantitative unit 154 via the reagent supply unit 153 and is quantified. The surplus second reagent 14 overflowing at the time of quantification in the reagent quantification unit 154 is accommodated in the reagent surplus unit 156 via the fifth passage 155.

  At the same time, in the second channel 200 shown in FIG. 8, the sample 11 flows into the sample determination unit 214 via the sample supply unit 213 and is quantified. The surplus sample 11 overflowed at the time of quantification in the sample quantification unit 214 is accommodated in the sample surplus unit 216 via the first passage 215. The first reagent 13 flows into the reagent quantification unit 234 via the reagent supply unit 233 and is quantified. The surplus first reagent 13 overflowed at the time of quantification in the reagent quantification unit 234 is accommodated in the reagent surplus unit 236 via the third passage 235. The second reagent 15 flows into the reagent quantitative unit 254 via the reagent supply unit 253 and is quantified. The surplus second reagent 15 overflowed at the time of quantification in the reagent quantification unit 254 is accommodated in the reagent surplus unit 256 via the fifth passage 255.

  In this embodiment, when the test chip 2 revolves around the main shaft 57 of the test chip 2 shown in FIG. 1, the front-rear direction in which the front surface 25 and the back surface 26 face each other and the axial direction of the support shaft 46 are parallel to each other. In the revolution state shown in FIG. 10, the quantification end portions 114 </ b> A and 114 </ b> B of the sample quantification unit 114 and the quantification end portions 214 </ b> A and 214 </ b> B of the sample quantification unit 214 are all located at the same distance from the main shaft 57. Therefore, the centrifugal force X applied to the two specimen quantification units 114 and 214 becomes equal, and the specimens 10 and 11 can be accurately quantified.

  Similarly, in the revolution state shown in FIG. 10, the quantitative end portions 134A and 134B of the reagent quantitative unit 134 and the quantitative end portions 234A and 234B of the reagent quantitative unit 234 are all located at the same distance from the main shaft 57. Therefore, the centrifugal force X applied to the two reagent quantification units 134 and 234 becomes equal, and the first reagent 12 and 13 can be accurately quantified. The fixed amount end portions 154A and 154B of the reagent fixed amount unit 154 and the fixed amount end portions 254A and 254B of the reagent fixed amount portion 254 are all located at the same distance from the main shaft 57. Therefore, the centrifugal force X applied to the two reagent quantification units 154 and 254 becomes equal, and the second reagents 14 and 15 can be accurately quantified.

  Furthermore, the inclination angle of the wall surface forming the concave portion of the sample quantifying unit 114 and the wall surface forming the concave portion of the sample quantifying unit 214 are equal when projected in the front-rear direction. Specifically, as shown in FIG. 7, the left wall surface 114C of the specimen quantifying unit 114 and the wall surface 115A of the first passage 115 extending in the lower left direction from the quantifying end portion 114A form an inclination angle θ11. As shown in FIG. 8, the left wall surface 214C of the specimen quantification unit 214 and the wall surface 215A of the first passage 215 extending from the quantification end 214A in the lower left direction form an inclination angle θ21. The inclination angle θ11 and the inclination angle θ21 are equal. As shown in FIG. 7, the right wall surface 114D of the sample quantifying unit 114 and the wall surface 117A of the second passage 117 extending in the upper right direction from the quantifying end portion 114B form an inclination angle θ12. As shown in FIG. 8, the right wall surface 214D of the specimen quantification unit 214 and the wall surface 217A of the second passage 217 extending in the upper right direction from the quantification end portion 214B form an inclination angle θ22. The inclination angle θ12 and the inclination angle θ22 are equal. Therefore, the specimens 10 and 11 can be moved from the specimen quantification units 114 and 214 without changing the direction of the centrifugal force X.

  Similarly, the wall surface forming the concave portion of the reagent quantitative unit 134 and the wall surface forming the concave portion of the reagent quantitative unit 234 have the same inclination angle when projected in the front-rear direction. Specifically, as shown in FIG. 7, the left wall surface 134C of the reagent quantitative unit 134 and the wall surface 135A of the third passage 135 extending in the lower left direction from the quantitative end part 134A form an inclination angle θ13. As shown in FIG. 8, the left wall surface 234C of the reagent quantification unit 234 and the wall surface 235A of the third passage 235 extending in the lower left direction from the quantification end 234A form an inclination angle θ23. The inclination angle θ13 and the inclination angle θ23 are equal. As shown in FIG. 7, the right wall surface 134D of the reagent quantitative unit 134 and the wall surface 137A of the fourth passage 137 extending in the upper right direction from the quantitative end part 134B form an inclination angle θ14. As shown in FIG. 8, the right wall surface 234D of the reagent quantitative unit 234 and the wall surface 237A of the fourth passage 237 extending in the upper right direction from the quantitative end portion 234B form an inclination angle θ24. The inclination angle θ14 and the inclination angle θ24 are equal. Therefore, the first reagents 12 and 13 can be moved from the reagent quantification units 134 and 234 without changing the direction of the centrifugal force X.

  The wall surface forming the concave portion of the reagent quantifying unit 154 and the wall surface forming the concave portion of the reagent quantifying unit 254 have the same inclination angle when projected in the front-rear direction. Specifically, as shown in FIG. 7, the left wall surface 154C of the reagent quantitative unit 154 and the wall surface 155A of the fifth passage 155 extending from the quantitative end 154A in the lower left direction form an inclination angle θ15. As shown in FIG. 8, the left wall surface 254C of the reagent quantification unit 254 and the wall surface 255A of the fifth passage 255 extending in the lower left direction from the quantification end 254A form an inclination angle θ25. The inclination angle θ15 and the inclination angle θ25 are equal. As shown in FIG. 7, the right wall surface 154D of the reagent quantitative unit 154 and the wall surface 157A of the sixth passage 157 extending from the quantitative end 154B in the upper right direction form an inclination angle θ16. As shown in FIG. 8, the right wall surface 254D of the reagent quantification unit 254 and the wall surface 257A of the sixth passage 257 extending in the upper right direction from the quantification end portion 254B form an inclination angle θ26. The inclination angle θ16 and the inclination angle θ26 are equal. Therefore, the second reagents 14 and 15 can be moved from the reagent quantification units 154 and 254 without changing the direction of the centrifugal force X.

  Next, the rotation controller 98 shown in FIG. 4 controls the stepping motor 51, whereby the test chip 2 in the revolution state shown in FIG. 10 is rotated 90 degrees in the clockwise direction when viewed from the front. As a result, the rotation angle of the inspection chip 2 returns to 0 degrees, and the centrifugal force X acts on the inspection chip 2 from the left side portion 23 toward the right side portion 22.

  Due to the action of the centrifugal force X, the specimen 10 quantified in the specimen quantification unit 114 moves to the second passage 117 in the first channel 100 shown in FIG. On the other hand, since the specimen surplus portion 116 is closed in the right direction, the surplus specimen 10 remains in the specimen surplus portion 116. Similarly, the first reagent 12 quantified in the reagent quantification unit 134 moves to the fourth passage 137, while the surplus first reagent 12 remains in the reagent surplus unit 136. The second reagent 14 quantified in the reagent quantification unit 154 moves to the sixth passage 157, while the surplus second reagent 14 remains in the reagent surplus unit 156.

  At the same time, in the second channel 200 shown in FIG. 8, the sample 11 quantified by the sample quantification unit 214 moves to the second passage 217. On the other hand, since the sample surplus portion 216 is closed in the right direction, the surplus sample 11 remains in the sample surplus portion 216. Similarly, the first reagent 13 quantified in the reagent quantification unit 234 moves to the fourth passage 237, while the surplus first reagent 13 remains in the reagent surplus unit 236. The second reagent 15 quantified in the reagent quantification unit 254 moves to the sixth passage 257, while the surplus second reagent 15 remains in the reagent surplus unit 256.

  Next, when the rotation controller 98 shown in FIG. 4 controls the stepping motor 51, as shown in FIG. 11, the test chip 2 in the revolution state is rotated 90 degrees counterclockwise as viewed from the front. As a result, the rotation angle of the inspection chip 2 changes to 90 degrees, and the centrifugal force X acts from the upper side portion 21 toward the lower side portion 24.

  Due to the action of the centrifugal force X, in the first channel 100 shown in FIG. 11, the specimen 10 that has moved to the second passage 117 and the first reagent 12 that has moved to the fourth passage 137 flow into the standby unit 171. In the standby unit 171, an intermediate liquid 16 in which the sample 10 and the first reagent 12 that have flown are mixed is generated by the action of the centrifugal force X. The second reagent 14 that has moved to the sixth passage 157 flows into the first reservoir 172. At the same time, in the second channel 200 shown in FIG. 8, the specimen 11 that has moved to the second passage 217 and the first reagent 13 that has moved to the fourth passage 237 flow into the standby unit 271. In the standby section 271, an intermediate liquid 17 in which the specimen 11 and the first reagent 13 shown in FIG. 11 are mixed is generated by the action of the centrifugal force X. The second reagent 15 that has moved to the sixth passage 257 flows into the second reservoir 272.

  Next, the rotation controller 98 shown in FIG. 4 controls the stepping motor 51, whereby the test chip 2 in the revolution state shown in FIG. 11 is rotated 90 degrees in the clockwise direction when viewed from the front. As a result, the rotation angle of the inspection chip 2 returns to 0 degrees, and the centrifugal force X acts on the inspection chip 2 from the left side portion 23 toward the right side portion 22.

  Due to the action of the centrifugal force X, in the first flow path 100 shown in FIG. 12, the intermediate liquid 16 stored in the standby unit 171 moves along the isolation wall 173 in the upper right direction. Furthermore, the intermediate liquid 16 moves from the upper end portion 173A of the isolation wall 173 in the same right direction as the centrifugal force X. The moving intermediate liquid 16 diffuses in contact with the stirring unit 180 and is evenly mixed into the second reagent 14 stored in the first storage unit 172. At the same time, in the second flow path 200 shown in FIG. 8, the intermediate liquid 17 stored in the standby unit 271 moves in the upper right direction along the isolation wall 273. Furthermore, the intermediate liquid 17 moves in the same right direction as the centrifugal force X from the upper end of the isolation wall 273. The moving intermediate liquid 17 diffuses in contact with the stirring unit 280 and is evenly mixed into the second reagent 15 stored in the second storage unit 272.

  With reference to FIG. 12, the above process will be described in more detail. The rotation angle of the test chip 2 in the revolution state shown in FIG. 11 changes from 90 degrees to 0 degrees. In this process, when the left wall surface 171A of the standby portion 171 becomes larger than 90 degrees with respect to the direction in which the centrifugal force X acts, the intermediate liquid 16 flows out from the upper end portion 173A in the direction in which the centrifugal force X acts. At the time when the outflow of the intermediate liquid 16 is started, the direction in which the centrifugal force X acts is equal to the direction of the line β. At this time, as shown by the black arrow in FIG. 12, the intermediate liquid 16 that has flowed out contacts the protruding end 183A of the protruding portion 183 located on the extension of the line β and diffuses.

  As the rotation angle of the inspection chip 2 approaches 0 degrees, the direction in which the intermediate liquid 16 flows out changes from the direction of the line β toward the direction of the line α. At this time, the position where the intermediate liquid 16 that has flowed out changes from the upper wall surface 183B of the protruding portion 183 to the lower wall surface 182C of the protruding portion 182. When the rotation angle of the inspection chip 2 reaches 0 degrees, the direction in which the centrifugal force X acts is equal to the direction of the line α. At this time, the intermediate liquid 16 that has flowed out contacts the protruding end 182A of the protruding portion 182 located on the extension of the line α and diffuses.

  As described above, the protrusions 182 and 183 are provided in the range from the position h1 to the position h2. As a result, even if the rotation angle of the inspection chip 2 changes from 90 degrees to 0 degrees, the intermediate liquid 16 that has flowed out of the upper end 173A always comes into contact with either of the protrusions 182 and 183. Therefore, the intermediate liquid 16 flowing into the first reservoir 172 is diffused and mixed well. As a result, the second liquid mixture 19 shown in FIG. 13 is efficiently generated.

  Furthermore, the projecting end 182A of the projecting portion 182 is located on the extension of the line α. The protruding end 183A of the protruding portion 183 is located on the extension of the line β. Thereby, in each protrusion end 182A, 183A, the intermediate | middle liquid 16 which flows into the 1st storage part 172 can be spread | diffused largely to an up-down direction. Further, the wall surface 182C of the protrusion 182 and the wall surface 183B of the protrusion 183 are inclined with respect to the direction of the centrifugal force X and the direction of the gravity Z. That is, each of the wall surfaces 182C and 183B is inclined with respect to the moving direction of the intermediate liquid 16 that flows into the first reservoir 172. Thereby, the intermediate liquid 16 flowing into the first reservoir 172 can be smoothly reflected and greatly diffused by the respective wall surfaces 182C and 183B. As a result, the intermediate liquid 16 is well mixed.

  As shown in FIG. 11, when the inspection chip 2 having a rotation angle of 90 degrees is revolved, the second reagent 14 is accumulated in the lower part of the first storage unit 172. As shown in FIG. 12, when the test chip 2 having a rotation angle of 0 degree is revolved, the second reagent 14 accumulates along the right wall surface 172 </ b> A of the first reservoir 172. In this embodiment, from the relationship between the volume of the first reservoir 172 and the amount of the second reagent 14 quantified in the reagent quantifier 154, the liquid level of the second reagent 14 corresponding to the rotation angle of the test chip 2 is determined. The position is known. The projecting ends 181 </ b> A to 183 </ b> A of the three projecting parts 181 to 183 are designed to be exposed to the outside of the liquid surface of the second reagent 14 regardless of the rotation angle of the test chip 2.

  Specifically, as shown in FIG. 11, the three protrusions 181 to 183 are provided above the liquid level in a state where the second reagent 14 is accumulated in the lower part of the first reservoir 172. Therefore, even when the test chip 2 having a rotation angle of 90 degrees is revolved, the protruding ends 181A to 183A of the protruding portions 181 to 183 are not covered with the liquid surface of the second reagent 14. Therefore, the intermediate liquid 17 flowing into the first reservoir 172 can be reliably contacted and diffused.

  As shown in FIG. 12, the three protrusions 181 to 183 are longer in the left-right direction than the liquid level in a state where the second reagent 14 is accumulated along the right wall surface 172 </ b> A of the first reservoir 172. That is, the projecting ends 181A to 183A of the projecting portions 181 to 183 are located on the left side of the liquid level. Therefore, even when the test chip 2 having a rotation angle of 0 degrees is revolved, the projecting ends 181A to 183A of the projecting parts 181 to 183 are not covered with the liquid surface of the second reagent 14, so It is possible to reliably contact and diffuse the flowing intermediate liquid 16.

  Next, when the rotation controller 98 shown in FIG. 4 controls the stepping motor 51, as shown in FIG. 13, the test chip 2 in the revolution state is rotated 90 degrees counterclockwise as viewed from the front. As a result, the rotation angle of the inspection chip 2 changes to 90 degrees, and the centrifugal force X acts from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, in the first flow path 100 shown in FIG. 12, the intermediate liquid 16 and the second reagent 14 are mixed in the first reservoir 172 to generate the first mixed liquid 18. At the same time, in the second flow path 200 shown in FIG. 8, the intermediate liquid 17 and the second reagent 15 are mixed in the second reservoir 272, and the second mixed liquid 19 shown in FIG. 13 is generated.

  As shown in FIG. 9, after execution of step S1, the first mixed solution 18 and the second mixed solution 19 are moved onto the measurement light (S3). That is, the revolution controller 97 shown in FIG. 4 controls the spindle motor 35 to move the inspection chip 2 to the measurement position. The rotation controller 98 shown in FIG. 4 controls the stepping motor 51 to change the rotation angle of the inspection chip 2 to 0 degrees. At this time, the direction in which the gravity Z acts is directed from the upper side 21 to the lower side 24 of the inspection chip 2. Since the first reservoir 172 is closed downward, the first liquid mixture 18 remains without moving from the first reservoir 172. Since the second reservoir 272 is closed downward, the second mixed liquid 19 remains without moving from the second reservoir 272.

  Next, optical measurement of the first mixed liquid 18 and the second mixed liquid 19 is performed (S5). Specifically, when the measurement controller 99 shown in FIG. 4 drives the measurement unit 7, the measurement light penetrates the first storage unit 172 and the second storage unit 272 in the front-rear direction. The CPU 91 shown in FIG. 4 measures the first mixed liquid 18 and the second mixed liquid 19 based on the change amount of the measurement light received by the sensor unit 72. In the present embodiment, different components are measured from the first mixed liquid 18 and the second mixed liquid 19 based on the wavelength of light transmitted through the first mixed liquid 18 and the second mixed liquid 19.

  Specifically, the first liquid mixture 18 is a liquid mixture for measuring glucose, and reacts with a light component having a wavelength of 340 nm. The second liquid mixture 19 is a liquid mixture for measuring total cholesterol, and reacts with a light component having a wavelength of 650 nm. As shown in FIG. 5, the light component having a wavelength of 340 nm passes through the band-pass filter 72B and is received by the light receiving sensor 72C. Based on the detection result of the light receiving sensor 72C, glucose is measured from the first mixed liquid 18. The light component having a wavelength of 650 nm passes through the band-pass filter 72D and is received by the light receiving sensor 72E. Based on the detection result of the light receiving sensor 72E, the total cholesterol is measured from the second mixed liquid 19. Finally, the inspection result measured in step S5 is displayed on the display 96 shown in FIG. 5 (S7). Thereafter, the main process is terminated.

<1-5. Main effects of the present embodiment>
According to the inspection chip 2 according to the first embodiment, the first reservoir 172 is provided on the front surface 25, and the second reservoir 272 is provided on the back surface 26. Since the two first storage parts 172 and the second storage part 272 are arranged on the front surface 25 and the back surface 26 of the test chip 2, the first mixed liquid 18 and the second liquid mixture 18 are suppressed while suppressing the total length of the liquid flow path of the test chip 2. The mixed liquid 19 can be inspected.

  Further, when the test chip 2 is rotated about the main shaft 57 and the front-rear direction of the test chip 2 and the axial direction of the support shaft 46 are parallel to each other, at least one of the quantitative end portions 114A and 114B of the sample quantitative unit 114 At least one of the quantitative end portions 214A and 214B of the sample quantitative unit 214 is located at an equal distance from the main shaft 57. That is, when the test chip 2 is rotated around the main shaft 57 in a state where the front-rear direction of the test chip 2 and the axial direction of the support shaft 46 are parallel, the sample to be quantified by the two sample quantification units 114 and 214. The liquid surfaces 10 and 11 are located at the same distance from the main shaft 57.

  Therefore, the centrifugal force X applied to the two specimen quantification units 114 and 214 becomes equal, and the specimens 10 and 11 can be accurately quantified. Similarly, the centrifugal force X applied to the two reagent quantification units 134 and 234 becomes equal, and the first reagents 12 and 13 can be accurately quantified. The centrifugal force X applied to the two reagent quantification units 154 and 254 becomes equal, and the second reagents 14 and 15 can be accurately quantified.

  The two specimen quantification units 114 and 214 have wall surfaces that form recesses capable of storing the specimens 10 and 11, and the inclination angles of the wall surfaces are equal when projected in the front-rear direction. Therefore, it is possible to increase the accuracy of the quantification in the sample quantification units 114 and 214 while suppressing the total length of the liquid channel. Similarly, the accuracy of quantification in each reagent quantification unit 134, 234 can be increased while the total length of the liquid channel is suppressed, and the accuracy of quantification in each reagent quantification unit 154, 254 can be increased.

  The first flow path 100 and the second flow path 200 have shapes that match when projected in the front-rear direction. Therefore, the reagent and the specimen can move in the same way in the first channel 100 and the second channel 200. As a result, since the two mixed liquids 18 and 19 can be inspected simultaneously while suppressing the total length of the liquid flow path, the inspection time can be shortened.

  The first flow path 100 is formed on the front surface 25 of the plate material 20, and the second flow path 200 is formed on the back surface 26 of the plate material 20. Therefore, the inspection chip 2 in which the first channel 100 and the second channel 200 are formed on both surfaces can be manufactured using one mold. As a result, the manufacturing cost of the inspection chip 2 in which the total length of the liquid flow path is suppressed can be reduced.

  In the inspection apparatus 1, since the inspection can be performed using the inspection chip 2 in which the first flow path 100 and the second flow path 200 are formed on both surfaces, a plurality of liquid flow paths provided in the inspection chip 2 are suppressed, while The mixed liquids 18 and 19 can be inspected. Different components are measured from the first mixed liquid 18 stored in the first storage section 172 and the second mixed liquid 19 stored in the second storage section 272 based on the wavelength of light. Therefore, the first mixed liquid 18 and the second mixed liquid 19 can be measured simultaneously with the common measurement light. As a result, since the two mixed liquids 18 and 19 can be inspected at the same time while suppressing the total length of the liquid flow path, the inspection time can be shortened.

<2. Second embodiment>
A second embodiment of the present invention will be described. Below, the same code | symbol as 1st embodiment is attached | subjected to the same structure as 1st embodiment, description is abbreviate | omitted, and only a different point from 1st embodiment is demonstrated.

  With reference to FIG. 7 and FIG. 14, the detailed structure of the test | inspection apparatus 1 and the test | inspection chip 2 which concern on 2nd embodiment is demonstrated. The inspection apparatus 1 according to the second embodiment is the same as the inspection apparatus 1 according to the first embodiment. However, the sensor unit 72 does not include the half mirror 72A, the band-pass filters 72B and 72D, and the light receiving sensor 72E shown in FIG. That is, the sensor unit 72 is configured by one light receiving sensor 72C.

  In the inspection chip 2 according to the second embodiment, the first channel 100 is provided on the front surface 25 and the second channel 200 is provided on the back surface 26 as in the first embodiment. The first channel 100 of the second embodiment is the same as the first channel 100 shown in FIG. On the other hand, as shown in FIG. 14, the second flow path 200 of the second embodiment is different in shape from the second flow path 200 shown in FIG.

  As shown in FIG. 14, the second channel 200 is similar to the first channel 100 shown in FIG. 8, the sample injection unit 211, the sample quantification unit 214, the sample surplus unit 216, the reagent injection unit 231, and the reagent quantification unit 234. , A reagent surplus part 236, a reagent injection part 251, a reagent quantitative part 254, a reagent surplus part 256, a standby part 271, and a second storage part 272.

  In the present embodiment, the reagent surplus portion 256 has a smaller volume than the first embodiment. That is, when viewed from the front, the reagent surplus portion 256 is smaller than the reagent surplus portion 156 provided on the surface 25 indicated by a dotted line in FIG. Thereby, the downstream end of the sixth passage 257 has a larger flow path area than the downstream end of the sixth passage 157 shown in FIG. Further, isolation walls 274 and 275 are provided instead of the isolation wall 273 and the agitation unit 280 shown in FIG.

  The isolation wall 274 is a wall portion that extends downward from the lower end portion of the right wall surface of the sixth passage 257. The isolation wall 275 is a wall portion that extends upward from the right end portion of the lower wall surface of the standby portion 271. The isolation wall 274 is provided above the isolation wall 275 and extends in parallel with the isolation wall 275. The left lower end portion of the isolation wall 274 and the upper right end portion of the isolation wall 275 have the same distance from the lower side portion 24. Between the isolation wall 274 and the isolation wall 275, a guide path 276 extending in the upper right direction from the lower portion of the standby portion 271 is formed. The upper right end portion of the guide path 276 is connected to the upper end portion of the second storage portion 272.

  Furthermore, the second flow path 200 includes a holding part 290 below the reagent surplus part 236. The holding part 290 is a part capable of holding the specimen 11 flowing through the second passage 217, and is a concave part opened to the left side. The second passage 217 extends to the upper left end of the holding part 290. The lower left end of the holding unit 290 is connected to the upper left end of the standby unit 271.

  In the present embodiment, the first reagent 12 and 13 and the second reagent 14 and 15 are enclosed in the inspection chip 2 in advance. As an example, the first reagent 12, 13 and the second reagent 14, 15 are all reagents for measuring total cholesterol. Before executing the test, the user injects the specimen 10 shown in FIG. 7 from the first inlet 191 shown in FIG. 14 and injects the specimen 11 shown in FIG. 16 from the second inlet 291. The first injection port 191 is a hole through which the sample 10 can be injected into the sample injection unit 111 shown in FIG. 7 and is formed in the left side portion 23. The second injection port 291 is a hole through which the sample 11 can be injected into the sample injection portion 211, and is formed in the upper side portion 21. That is, the first inlet 191 and the second inlet 291 are formed on different surfaces of the inspection chip 2. Thereby, it is possible to prevent erroneous insertion of the specimens 10 and 11 into the first channel 100 and the second channel 200 while suppressing the total length of the liquid channel. Note that the specimen 10 and the specimen 11 are different specimens.

  With reference to FIGS. 15-18, the test | inspection method using the test | inspection apparatus 1 and test | inspection chip 2 which concern on 2nd embodiment is demonstrated. In the present embodiment, the main process executed in the inspection apparatus 1 is different from the main process shown in FIG.

  As shown in FIG. 15, in the main process of the second embodiment, the generation of the first mixed liquid 18 is first executed by the same procedure as Step S1 (S51). In the present embodiment, after the sample and the reagent are quantified in the first channel 100 and the second channel 200, the following processing is performed. That is, when the rotation controller 98 controls the stepping motor 51, the test chip 2 in the revolution state shown in FIG. 10 is rotated 90 degrees counterclockwise as viewed from the front. As a result, the rotation angle of the inspection chip 2 returns to 0 degrees, and the centrifugal force X acts on the inspection chip 2 from the left side portion 23 toward the right side portion 22.

  Due to the action of the centrifugal force X, the specimen 10 quantified in the specimen quantification unit 114 moves to the second passage 117 in the first channel 100 shown in FIG. Furthermore, the sample 10 flows into the first storage unit 172 via the standby unit 171. Similarly, the first reagent 12 quantified by the reagent quantification unit 134 moves to the fourth passage 137. Furthermore, the first reagent 12 flows into the first storage unit 172 via the standby unit 171. The second reagent 14 quantified in the reagent quantification unit 154 moves to the sixth passage 157 and flows into the first storage unit 172. In the first reservoir 172, the sample 10, the first reagent 12, and the second reagent 14 that flowed in are mixed by the action of the centrifugal force X, and the first mixed liquid 18 shown in FIG. 13 is generated.

  On the other hand, in the second channel 200 as shown in FIG. 16, the sample 11 quantified in the sample quantification unit 214 is moved to the second passage 217 and flows into the holding unit 290 by the action of the centrifugal force X. Since the holding unit 290 is closed in the right direction, the sample 11 that has flowed in remains in the holding unit 290. At the same time, the first reagent 13 quantified in the reagent quantification unit 234 moves to the fourth passage 237. Further, the first reagent 13 that has moved through the fourth passage 237 flows into the sixth passage 257 along the isolation wall 274. The second reagent 15 quantified by the reagent quantification unit 254 also moves to the sixth passage 157. In the rightmost sixth passage 157, the inflowing first reagent 13 and second reagent 15 are mixed by the action of the centrifugal force X, and the intermediate liquid 17 is generated.

  Next, the revolution controller 97 shown in FIG. 4 controls the spindle motor 35 to stop the revolution. As a result, the first liquid mixture 18 is moved onto the measuring light by the gravity Z, and at the same time, the second liquid mixture 19 is generated (S53). That is, in step S53, the same process as step S3 described above is executed. Thereby, as shown in FIG. 17, the test | inspection chip 2 is revolved to a measurement position. At this time, the direction in which the gravity Z acts is directed from the upper side 21 to the lower side 24 of the inspection chip 2. Since the first reservoir 172 shown in FIG. 7 is closed downward, the first mixed liquid 18 remains without moving from the first reservoir 172. On the other hand, as shown in FIG. 17, in the second flow path 200, the sample 11 held in the holding unit 290 moves to the standby unit 271 by the action of gravity Z. The intermediate liquid 17 generated in the sixth passage 157 moves to the standby unit 271 along the isolation wall 274 by the action of gravity Z. In the standby unit 271, the sample 11 and the intermediate liquid 17 that have flowed in are mixed to generate the second mixed liquid 19. Since the standby unit 271 is closed downward, the generated second mixed liquid 19 remains without moving from the standby unit 271.

  Next, optical measurement of the first liquid mixture 18 is executed (S55). Specifically, when the measurement controller 99 drives the measurement unit 7, the CPU 91 calculates the amount of change in the measurement light received by the sensor unit 72. The calculated change amount is a change amount of the measurement light transmitted through the first mixed liquid 18. Therefore, the total cholesterol of the first mixed liquid 18 is acquired based on the calculated amount of change.

  Next, the second mixed liquid 19 is moved onto the measurement light (S57). That is, in step S57, the revolution controller 97 shown in FIG. 4 controls the spindle motor 35, whereby the stationary inspection chip 2 shown in FIG. 17 is revolved. Thereby, the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22. Due to the action of the centrifugal force X, the second mixed liquid 19 stored in the standby unit 271 moves along the isolation wall 275 along the guide path 276 and flows into the rightmost second storage unit 272. Further, the revolution controller 97 shown in FIG. 4 controls the spindle motor 35, whereby the inspection chip 2 revolves to the measurement position and then stops the revolution. At this time, the direction in which the gravity Z acts is directed from the upper side portion 21 to the lower side portion 24 of the inspection chip 2. Thereby, as shown in FIG. 18, the second liquid mixture 19 is stored in the lower portion of the second storage portion 272.

  Next, optical measurement of the second liquid mixture 19 is performed (S59). Specifically, when the measurement controller 99 shown in FIG. 4 drives the measurement unit 7, the CPU 91 calculates the amount of change in the measurement light received by the sensor unit 72. The calculated change amount is a change amount of the measurement light transmitted through the first mixed solution 18 and the second mixed solution 19. Therefore, the total cholesterol of the second mixed liquid 19 is acquired based on a value obtained by subtracting the extrapolated amount of the change amount calculated in step S55 from the calculated change amount. For example, the extrapolation amount is an expected change amount of the first liquid mixture 18 calculated based on the elapsed time from step S55 to step S59. Finally, the inspection results measured in steps S55 and S59 are displayed on the display 96 shown in FIG. 5 (S61). Thereafter, the main process is terminated.

  According to the test | inspection chip 2 which concerns on 2nd embodiment, there exists an effect similar to 1st embodiment. Furthermore, the second flow path 200 includes a standby unit 271 that can store the second mixed liquid 19. The standby unit 271 is provided downstream of the quantification units 214, 234, and 245 in the direction of movement of the specimen or reagent, and upstream of the second storage unit 272 in the direction of movement of the sample or reagent. . By providing the standby section 271 in the second flow path 200, the timing at which the first mixed liquid 18 is stored in the first storage section 172 of the first flow path 100 and the second storage section 272 of the second flow path 200 The timing at which the two liquid mixture 19 is stored can be shifted. Since the mutual measurement does not interfere even with common measurement light while suppressing the overall length of the liquid flow path, the inspection accuracy can be increased.

  In the inspection apparatus 1, since the inspection can be performed using the inspection chip 2 in which the first flow path 100 and the second flow path 200 are formed on both surfaces, a plurality of liquid flow paths provided in the inspection chip 2 are suppressed, while The mixed liquids 18 and 19 can be inspected. The first liquid mixture 18 stored in the first storage part 172 and the second liquid mixture 19 stored in the second storage part 272 can be measured at different timings by the common measurement light. As a result, it is possible to increase the inspection accuracy because the mutual measurement does not interfere even with common measurement light while suppressing the total length of the liquid flow path.

<3. Other>
In the above embodiment, the main shaft 57 corresponds to the “first shaft” of the present invention, and the support shaft 46 corresponds to the “second shaft” of the present invention. The first reservoir 172 and the second reservoir 272 correspond to “two reservoirs” of the present invention. The sample quantification units 114 and 214 correspond to the “two quantification units” of the present invention, the sample quantification unit 114 corresponds to the “first quantification unit” of the present invention, and the sample quantification unit 214 corresponds to the “second quantification unit” of the present invention. Corresponds to “quantitative section”. Similarly, the reagent quantification units 134 and 234 correspond to “two quantification units” of the present invention, the reagent quantification unit 134 corresponds to the “first quantification unit” of the present invention, and the reagent quantification unit 234 of the present invention. Corresponds to the “second quantitative unit”. The reagent quantification units 154 and 254 correspond to the “two quantification units” of the present invention, the reagent quantification unit 154 corresponds to the “first quantification unit” of the present invention, and the reagent quantification unit 254 corresponds to the “second quantification unit” of the present invention. Corresponds to “quantitative section”.

  The spindle motor 35 and the stepping motor 51 correspond to the “rotating part” of the present invention. In the first embodiment, the CPU 91 executing step S1 corresponds to the “liquid mixing unit” of the present invention. The CPU 91 executing step S5 corresponds to the “measurement control unit” of the present invention. In the second embodiment, the CPU 91 executing step S51 corresponds to the “first liquid mixing unit” of the present invention. The CPU 91 executing step S53 corresponds to the “second liquid mixing unit” of the present invention. The CPU 91 executing step S55 corresponds to the “first measurement control unit” of the present invention. The CPU 91 executing step S59 corresponds to the “second measurement control unit” of the present invention.

  The present invention is not limited to the above embodiment, and various modifications can be made. The inspection apparatus 1 and the inspection chip 2 of the above embodiment are merely examples, and the structure, shape, processing, and the like of each can be changed.

(1) In the above embodiment, the combination of the sample quantification units 114 and 214, the combination of the reagent quantification units 134 and 234, and the combination of the reagent quantification units 154 and 254 are provided as two quantification units on the test chip 2. ing. It is not limited to this example. The quantity of the two quantitative units can be changed according to the quantity of the specimen or reagent injected into the test chip 2.

(2) In the above embodiment, the two quantitative end portions 114A and 114B included in the sample quantitative unit 114 and the two quantitative end portions 214A and 214B included in the sample quantitative unit 214 are all equidistant from the main shaft 57 during revolution. Located in. The present invention is not limited to this example, and at least one of the fixed amount end portions 114A and 114B and at least one of the fixed amount end portions 214A and 214B may be located at an equal distance from the main shaft 57 at the time of revolution. For example, if the fixed amount end portion 114A and the fixed amount end portion 214A are equidistant from the main shaft 57, the fixed amount end portion 114B and the fixed amount end portion 214B may have different distances from the main shaft 57. In this case, because the surplus samples 10 and 11 flow to the sample surplus parts 116 and 136, respectively, at the time of quantification in the sample quantification units 114 and 214, the amount of liquid quantified in the sample quantification units 114 and 214 does not change. The same applies to the reagent quantification units 134 and 234 and the reagent quantification units 154 and 254.

  It is sufficient that at least one of the fixed amount end portions 114A and 114B and at least one of the fixed amount end portions 214A and 214B have a mirror image relationship between the front surface 25 and the back surface 26. The same applies to the reagent quantification units 134 and 234 and the reagent quantification units 154 and 254. Therefore, parts other than the fixed end part do not have to be mirror images. For example, the sample quantification unit 114 and the sample quantification unit 214 may have shapes with different depths, for example. Furthermore, in 1st embodiment, the position of the 1st storage part 172 and the 2nd storage part 272 should just be a mirror image relationship with the surface 25 and the back surface 26. FIG.

(3) In the above embodiment, the surplus portions 116, 136, and 156 of the first flow channel 100 and the surplus portions 216, 236, and 256 of the second flow channel 200 are independent of each other. The test chip is not limited to this example, and the test chip may include a common surplus part that can accommodate the sample or reagent that has flowed out from the two quantification parts. For example, the specimen surplus part 116 and the specimen surplus part 216 may be one surplus part that penetrates the plate member 20 in the front-rear direction. In this case, it is possible to reduce the possibility that the specimen or reagent overflows from the surplus portion while suppressing the overall length of the liquid flow path. Therefore, it is possible to reduce the possibility that the overflowing specimen or reagent is mixed into the solution to be measured and the inspection accuracy is lowered.

(4) In the above embodiment, the injection portions 111, 131, 151 of the first flow channel 100 and the injection portions 211, 231, 251 of the second flow channel 200 are independent of each other. The test chip is not limited to this example, and the test chip may share an injection part into which a common reagent or specimen is injected by the first channel and the second channel. For example, the sample injection part 111 and the sample injection part 211 may be one injection part that penetrates the plate member 20 in the front-rear direction. In this case, by sharing the injection part, the volume of the injection part can be increased as compared with the case where two injection parts are provided separately.

(5) In the second embodiment, the first inlet 191 is provided on the left side 23 and the second inlet 291 is provided on the upper side 21. Without being limited to this example, the first injection port and the second injection port may be formed on different surfaces of the inspection chip. The first injection port and the second injection port are not limited to the sample injection port, and may be a reagent injection port.

(6) Although the inspection chip 2 includes the plate material 20 and the sheet 29, the inspection chip 2 may not include the sheet 29. For example, you may use the test | inspection chip 2 in which the 1st flow path 100 and the 2nd flow path 200 were directly formed in the board | plate material 20. FIG. The number of reagents injected into the test chip 2 is not limited to two, and may be one reagent or three or more reagents.

DESCRIPTION OF SYMBOLS 1 Test | inspection apparatus 2 Test | inspection chip 7 Measuring part 10 Specimen 11 Specimen 12 1st reagent 13 1st reagent 14 2nd reagent 15 2nd reagent 18 1st liquid mixture 19 2nd liquid mixture 20 Plate material 25 Front surface 26 Back surface 35 Spindle motor 46 Support Shaft 51 Stepping motor 91 CPU
100 First channel 114 Specimen quantification unit 114A Quantification end 114B Quantification end 134 Reagent quantification unit 134A Quantification end 134B Quantification end 154 Reagent quantification unit 154A Quantification end 154B Quantification end 172 First reservoir 191 First inlet 200 Second channel 214 Specimen quantification unit 214A Quantification end 214B Quantification end 234 Reagent quantification unit 234A Quantification end 234B Quantification end 254 Reagent quantification unit 254A Quantification end 254B Quantification end 272 Second reservoir 291 Second note entrance

Claims (8)

A liquid specimen and reagent are injected, and a centrifugal force is applied by being rotated about a predetermined first axis, and by rotating about a second axis different from the first axis, the above-mentioned An inspection chip whose direction of centrifugal force is changed,
Two reservoirs that are each capable of storing a mixed liquid of the specimen and the reagent that flows in, and that is a part where the stored mixed liquid is measured;
Two quantification units including a space capable of quantifying the amount of the sample or the reagent flowing into each of the two storage units, and
The two reservoirs are a first reservoir provided on one of the front and back surfaces of the inspection chip, and a second reservoir provided on the other of the front and back surfaces,
The two quantification units are a first quantification unit and a second quantification unit each having two quantification end portions where a liquid surface of the specimen or the reagent is formed when the liquid amount is quantified,
In the state in which the inspection chip is rotated about the first axis and the front and back directions, which are the directions in which the front surface and the back surface face each other, and the axial direction of the second axis are parallel to each other, An inspection chip, wherein at least one of the two quantitative end portions provided in the section and at least one of the two fixed end portions provided in the second quantitative section are located at an equal distance from the first axis.   The two quantification units each include a wall surface that forms a recess capable of storing the specimen or the reagent, and the inclination angles of the wall surfaces are equal when projected in the front-back direction. Item 2. The inspection chip according to Item 1.   3. The test chip according to claim 1, further comprising a common surplus part that communicates with the two quantification units and can store the specimen or the reagent that has flowed out of each of the quantification units. A first channel including the first reservoir and the first quantification unit, the specimen and the reagent being movable;
Including the second reservoir and the second quantitative unit, and a second flow path through which the specimen and the reagent can move,
4. The inspection chip according to claim 1, wherein the first flow path and the second flow path have shapes that match when projected in the front-back direction. 5. A first channel including the first reservoir and the first quantification unit, the specimen and the reagent being movable;
Including the second reservoir and the second quantitative unit, and a second flow path through which the specimen and the reagent can move,
The second flow path is provided on the downstream side in the movement direction of the sample or the reagent with respect to the second quantification unit, and on the upstream side in the movement direction of the sample or the reagent with respect to the second storage unit. The inspection chip according to claim 1, further comprising a standby unit that can store the mixed liquid. Comprising one substrate forming the front surface and the back surface;
The first flow path is formed on the surface of the substrate;
The test chip according to claim 4, wherein the second flow path is formed on the back surface of the substrate. A first inlet capable of injecting the specimen or the reagent into the first channel;
A second injection port capable of injecting the specimen or the reagent into the second channel;
The inspection chip according to claim 4, wherein the first injection port and the second injection port are formed on different surfaces of the inspection chip. An inspection apparatus capable of using an inspection chip into which a liquid specimen and reagent are injected,
The inspection chip is
The sample and the reagent are movable, and a first flow path provided on one of the front surface and the back surface of the test chip;
The specimen and the reagent are movable, and comprise a second flow path provided on the other of the front surface and the back surface,
The first channel includes a first reservoir that can store a mixed solution of the sample and the reagent injected into the first channel;
The second channel includes a second reservoir that can store a mixed solution of the sample and the reagent injected into the second channel,
The inspection device includes:
A measuring unit capable of performing optical measurement by emitting light to an object;
A rotating part capable of rotating the inspection chip around a predetermined first axis and a second axis different from the first axis ;
By rotating the test chip by the rotating part, the first mixed liquid that is the mixed liquid of the specimen and the reagent that moves in the first flow path is generated, and the second liquid flow path moves in the second flow path A liquid mixing means for generating a second mixed liquid that is the mixed liquid of the specimen and the reagent;
When the first mixed liquid and the second mixed liquid are generated by the liquid mixing unit, the first mixed liquid stored in the first storage part and the first storage liquid stored in the second storage part A measurement control means for optically measuring the second mixed liquid simultaneously by the measurement unit,
The measurement control means measures different components from the first mixed solution and the second mixed solution based on the wavelength of the light, respectively.

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