JP5610681B2 - Neutral fat accumulation inhibitor - Google Patents

Description

  The present invention relates to a humectant, an anti-aging agent, an antioxidant, a neutral fat accumulation inhibitor, a whitening agent and an anti-inflammatory agent. More specifically, it contains a moisturizing agent, a cell activator, an antioxidant, a neutral fat accumulation inhibitor, a whitening agent and an anti-inflammatory agent, and an extract of the genus Makiaceae. The present invention relates to an external preparation for skin and an oral preparation.

  Wrinkles, tarmi, pigmentation / color change, skin elasticity decline, skin surface morphology disorder, and other factors causing deterioration of skin symptoms include, for example, wrinkles and tarmi, a decrease in dermal fibroblast function due to drying, aging, etc. In addition, there are important factors such as reduction or degeneration of the dermal matrix such as collagen and elastin, and oxidative damage caused by external stress such as ultraviolet rays. In addition, pigmentation and color change, including skin darkness, are partly unknown, but are caused by hormonal abnormalities and melanin production by the stimulation of ultraviolet rays of sunlight. Among them, spots and freckles are melanin. It is believed that abnormal pigmentation is the cause.

  In order to solve such a problem, various methods have been studied conventionally. For example, as a cell activator, essence of Ponkan (see Patent Document 1), as an antioxidant, Asteraceae heterotheca plant extract (see Patent Document 2), and the like, and as a melanin production inhibitor, Ginger genus Plant extracts (see Patent Document 3) and the like are known.

  Prior art related to moisturizers, anti-aging agents, antioxidants, neutral fat accumulation inhibitors, whitening agents, anti-inflammatory agents, skin external preparations containing these, and oral preparations containing extracts of the genus Maki family Was not recognized.

JP 2001-131045 A JP-A-11-180886 JP 2000-159626 A

  Naturally-derived components are known to have various pharmacological and cosmetic effects, and so far many plants and fungi have been widely applied to fields such as external preparations for skin and oral use. However, there are many naturally-derived ingredients whose effects are not yet known, and the development of effective ingredients having excellent moisturizing action, anti-aging action, antioxidant action, whitening action, etc. was expected. . The present invention was made to find such an active ingredient, and is a moisturizer, anti-aging agent, antioxidant, neutral fat accumulation inhibitor, which can be widely applied to skin external preparations and oral preparations, It is an object to provide a whitening agent, an anti-inflammatory agent, a skin external preparation containing these, and an oral preparation.

  In order to find a moisturizer, an anti-aging agent, an antioxidant, a neutral fat accumulation inhibitor, a whitening agent and an anti-inflammatory agent that can be widely applied to external preparations for skin, oral preparations, etc. We have intensively studied various substances derived from it. As a result, the present inventors have found a moisturizing action, an anti-aging action, an antioxidant action, a neutral fat accumulation suppressing action, a whitening action, and an anti-inflammatory action that are excellent in extracts of the genus Maki family, and have completed the present invention. That is, the present invention relates to a moisturizer, an anti-aging agent, an antioxidant, a neutral fat accumulation inhibitor, a whitening agent and an anti-inflammatory agent containing an extract of the periaceae plant, and 1 The present invention provides an external preparation for skin and an oral preparation containing extracts of seeds or two or more kinds of plants.

  According to the present invention, it is possible to provide a moisturizer, an anti-aging agent, an antioxidant, a neutral fat accumulation inhibitor, a whitening agent and an anti-inflammatory agent having excellent effects. Moreover, the skin external preparation and oral preparation which contain these as an active ingredient can be provided.

About 100 species of plants of the genus Podocarpus used in the present invention are known. For example, Japanese plover ( Podocarpus macrophyllus D. Don.), Nagi ( Podocarpus nagi ), Podocarpus nerifolios ( Podocarpus nerifolios D. Don.), Etc. are known.

The plant used as the raw material used in the present invention is not particularly limited as long as it is a plant belonging to the genus Macchiaceae. It is particularly preferable from the viewpoint of effectiveness to use the periwinkle plant, Podocarpus macrophyllus D. Don.

  In the present invention, as the extract of the genus Macchiaceae, the raw material or dried and pulverized one can be used as it is, but an extract extracted with a solvent or the like can also be used. At the time of extraction, it may be used as it is, but considering the extraction efficiency, it is desirable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by a general method such as a method of immersing in an extraction solvent or an extraction method using a supercritical fluid or the like. The extraction temperature is preferably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is preferably about 1 hour to 14 days.

  Examples of the extraction solvent include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, ethyl ether, propyl ether, and the like. Solvents such as ethers, esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia. It is also possible to extract under pressure using an autoclave or the like.

  The obtained extract can be used as it is, but the concentrated and dried product can be dissolved again in water or a solvent, or purified such as decolorization, deodorization, and desalting as long as these physiological functions are not impaired. You may use after performing a process or performing the fractionation process by column chromatography. For storage, it can be freeze-dried after purification and dissolved in a solvent when used.

  When using a periaceae plant, the site of use is not particularly limited, and the whole or all parts such as flowers, leaves, stems, branches, roots, seeds, bark, sap, pericarp, and fruit can be used. I do not care. From the viewpoint of availability and effectiveness, it is more preferable to use leaves and fruits.

  The periwinkle plant has superior moisturizing, anti-aging, antioxidant, neutral fat accumulation, whitening, anti-inflammatory, moisturizer, anti-aging agent, antioxidant, neutral It can be used as a fat accumulation inhibitor, whitening agent, and anti-inflammatory agent. In addition, moisturizers, anti-aging agents, antioxidants, neutral fat accumulation inhibitors, whitening agents, and anti-inflammatory agents containing extracts from the genus Maki are not only applied to the skin but also to the hair. It can also be used for oral administration and can be applied to pharmaceuticals, quasi-drugs, cosmetics or oral preparations.

  A moisturizing agent containing an extract of the genus Macchiaceae exhibits an excellent moisturizing action on the skin and hair, and has a particularly high moisturizing effect on the skin.

  An anti-aging agent containing an extract of the genus Macchiaceae exhibits an excellent cell activation effect on various cells, but particularly has an excellent effect on dermal fibroblasts and human epidermal keratinocytes. It is useful as a dermal fibroblast activator and a human epidermal keratinocyte activator.

  An antioxidant containing an extract of the genus Macchiaceae exhibits an excellent antioxidant action and is useful as an antioxidant.

  A whitening agent containing an extract of the plant belonging to the genus Macchiaceae exhibits an excellent whitening action, but particularly exhibits an excellent effect on the melanin production promoting action and is useful as a whitening agent.

  An anti-inflammatory agent containing an extract of the genus Macchiaceae exhibits an excellent anti-inflammatory action and is useful as an anti-inflammatory agent.

  A neutral fat accumulation inhibitor containing an extract of the plant belonging to the genus Macchiaceae exhibits an excellent neutral fat accumulation inhibitory action and is useful as a neutral fat accumulation inhibitor. Hyperlipidemia, arteriosclerosis, fatty liver, etc. are known as diseases caused by excessive accumulation of triglycerides. Neutral fat accumulation suppression using extracts of periwinkle plants as active ingredients The agent can be expected not only to prevent and improve obesity but also to prevent and improve such diseases. A slimming effect can also be expected.

  In addition, by incorporating an extract of the plant belonging to the genus Makiaceae into a topical skin preparation, prevention and improvement of skin symptoms such as wrinkles, tarmi, skin firmness, spots, kusumi, dryness, fine lines, abdomen, thighs, A skin external preparation that exhibits an excellent effect in preventing and improving partial obesity on the face, etc. can be obtained. Skin external preparation for moisturizing, skin external preparation for improving anti-aging, skin external preparation for whitening, or neutral fat It can also be used as a skin external preparation for suppressing accumulation. Furthermore, periwinkle plants can also be used in oral preparations such as pharmaceuticals, quasi drugs, and oral preparations for the purpose of beauty, health maintenance or nutritional supplementation.

  The compounding amount of the extract of the genus Macchiaceae can be adjusted depending on the type of skin external preparation or oral preparation and the purpose of use. From such points, 0.0001 to 50.0% by mass is preferable with respect to the total amount, and more preferably 0.001 to 25.0% by mass.

  When the extract of the genus Makiaceae is added to the external preparation for skin, the dosage form is arbitrary. For example, it is provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. be able to. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  For the topical skin preparation containing the extract of the genus Makiaceae, oily ingredients, which are usually blended in pharmaceuticals, quasi drugs, skin cosmetics, hair cosmetics and cleaning agents, as necessary, Other ingredients such as humectants, powders, pigments, emulsifiers, solubilizers, detergents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and fungicides, alcohols, etc. it can. In addition, other moisturizers, cell activators, antioxidants, slimming agents, anti-aging agents, whitening agents, and anti-inflammatory agents can be used in combination as long as the effects of the present invention are not impaired.

  When the extract of the genus Macchiaceae is combined with an oral preparation, its form is not particularly limited, but liquid forms such as liquids, solid preparations such as granules, tablets, capsules, glazes, or jelly, It can be processed and used in various forms such as gummi and gum, and can be used as pharmaceuticals, quasi drugs, oral supplements for nutritional supplements, oral health supplements and the like. In doing so, other additives such as excipients, binders, extenders, disintegrants, surfactants, lubricants, dispersants, buffers, preservatives, coating agents, preservatives, flavoring agents, flavoring agents. Coloring agents, plasticizers and the like can be added. In addition, other moisturizers, cell activators, antioxidants, slimming agents, anti-aging agents, whitening agents, and anti-inflammatory agents can be used in combination as long as the effects of the present invention are not impaired.

  In the following, production examples of extracts of the genus Macchiaceae, test for evaluating each action, formulation examples of external preparations for skin and oral preparations will be described in more detail, but the technical scope of the present invention is this. It is not limited at all.

[Production Example 1]
100 g of dried pulverized leaves of Inoki maki were dispersed in 2.0 kg of purified water and extracted by heating at 120 ° C. for 20 minutes using an autoclave. The extract supernatant was filtered and lyophilized to obtain extract 1.

[Production Example 2]
100 g of dried pulverized leaves of Eustoma grandiflorum were dispersed in 2.0 kg of 50 vol% ethanol aqueous solution and extracted at room temperature for 2 hours with stirring. The extract supernatant was filtered off, concentrated under reduced pressure, and lyophilized to obtain extract 2.

[Production Example 3]
100 g of a dried pulverized product of Inuma persimmon was dispersed in 2.0 kg of a 50% by volume ethanol aqueous solution and extracted at room temperature for 2 hours with stirring. The extract supernatant was filtered off, concentrated under reduced pressure, and then lyophilized to obtain extract 3.

  Using the above production example, the effectiveness of the extract of the genus Macchiaceae was evaluated.

  The moisturizing effect of the extract of the genus Maki is shown. Evaluation was performed using Extract 1 (Inumakaki (leaf) hot water extract) as a sample.

<Human dermal fibroblast hyaluronic acid production promoting action (moisturizing effect)
The evaluation was performed according to the following procedure.
Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After 24 hours, the medium was replaced with 0.5% by mass FBS-added DMEM medium containing a sample adjusted to an arbitrary concentration, and further cultured for 3 days. For the quantification of hyaluronic acid secreted into the culture supernatant, an indirect ELISA method using proteoglycan was used, and finally 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid against labeled peroxidase. ) After diammonium salt (ABTS) and hydrogen peroxide were added and reacted, the absorbance at 405 nm was measured with a microplate reader. The amount of protein in each well was measured using BCA Protein Assay Kit manufactured by PIERCE, and the amount of hyaluronic acid produced per unit amount of protein was determined. The evaluation results are shown in Table 1 as relative values with the amount of hyaluronic acid produced per unit protein in the control with no sample added as 100.
In the table, * and ** indicate a significance probability of less than 5% (P <0.05), and a significance probability of less than 1% (P <0.01), respectively, with respect to the significance P value in the t-test. This is indicated by *. Hereinafter, the same applies to * and ** in the table.

  As is clear from Table 1, the extract of the genus Makiaceae plant showed a significant human dermal fibroblast hyaluronic acid production promoting action. From this, it was clarified that the plant belonging to the genus Makiaceae has an excellent moisturizing effect because it has an effect of promoting hyaluronic acid production in human dermal fibroblasts.

  It shows about the anti-aging effect of the extract of the genus Maki. As a sample, the evaluation was performed using the extract 2 (Inumaki (leaf) 50 vol% ethanol extract).

<Human dermal fibroblast activation (anti-aging effect)>
Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 1% by weight fetal bovine serum (FBS) was used as the seeding medium. After 24 hours, the medium was replaced with a DMEM medium supplemented with 1% mass FBS to which a sample having a concentration shown in Table 2 was added, and further cultured for 48 hours. Next, the MTT reagent adjusted with the culture medium so that it might become 400 micrograms / mL was added to the cell except the supernatant, and it culture | cultivated for about 2 hours. Finally, formazan produced in 2-propanol was extracted and the absorbance at 550 nm was measured. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated using the difference between the two measured values. In the evaluation, in addition to the sample culture solution, 1% FBS-added DMEM medium was used as a control, and 5% by mass FBS-added DMEM medium was used as a positive control. The evaluation results are shown in Table 2 as relative values with the cell activation effect in the control with no sample added being taken as 100.

  As is clear from Table 2, a significant human dermal fibroblast activation effect was observed in the extract of the genus Maki family. From this, it was clarified that the plant belonging to the genus Maki belongs to the genus Maki family and has an excellent anti-aging effect.

<Activation of human epidermal keratinocytes (anti-aging effect)>
Human epidermal keratinocytes HaCaT were seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ cells per well. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After 24 hours, the sample adjusted to an arbitrary concentration was replaced with 5% by mass FBS-added DMEM medium, and further cultured for 24 hours. Next, the MTT reagent adjusted with the culture medium so that it might be set to 100 microgram / mL was added to the cell except the supernatant, and it culture | cultivated for about 2 hours. Finally, formazan produced in 2-propanol was extracted and the absorbance at 550 nm was measured. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated using the difference between the two measured values. The evaluation results are shown in Table 3 as relative values when the cell activation effect in the control with no sample added is taken as 100.

  As is clear from Table 3, a significant human epidermal keratinocyte activation effect was observed in the extract of the genus Maki family. From this, it was clarified that the plant belonging to the genus Makiaceae has an excellent anti-aging effect because it has a human keratinocyte activation effect.

  The radical scavenging effect of an extract of the genus Maki is shown. As a sample, the evaluation was performed using the extract 2 (Inumaki (leaf) 50 vol% ethanol extract).

<DPPH radical scavenging action (antioxidant action)>
100 μL of 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH) ethanol solution was added to 100 μL of a sample solution prepared to an arbitrary concentration with 50% by mass ethanol and mixed well. And the absorbance at 516 nm derived from the DPPH radical was measured. The DPPH radical elimination rate is defined by equation (1), where (A) is the absorbance when no sample is added and (B) is the absorbance when the sample is added.
Formula (1) Erase rate = {1- (B) / (A)} × 100
The evaluation results are shown in Table 4.

  As is clear from Table 4, a significant DPPH radical scavenging action was observed in the extract of the genus Maki family. From this, it was clarified that the plant belonging to the genus Makiaceae has a DPPH radical scavenging action and an excellent antioxidant action.

<SOD-like activity (antioxidation)>
To 75 μL of the HANK ′S (+) solution containing 0.25 mM WST-1 and 1 mM Hypoxanthine, 25 μL of the sample solution prepared to an arbitrary concentration with the HANK ′S (+) solution was added. Further, 25 μL (0.0075Units) of Xanthine Oxidase was added, and after reacting at 37 ° C. for 15 minutes, the absorbance at 450 nm was measured. When the absorbance when no sample is added is (A) and the absorbance when the sample is added is (B), the superoxide anion elimination rate is defined by equation (2).
Formula (2) Erase rate (%) = {1- (B) / (A)} × 100
The evaluation results are shown in Table 5.

  As is clear from Table 5, a significant SOD-like activity was observed in the extract of the genus Maki family. From this, it was clarified that SOD-like activity was observed in the genus Maki family, and it had an excellent antioxidant effect.

  It shows about the neutral fat accumulation inhibitory effect of the extract of the genus Maki. Evaluation was performed using Extract 3 (Inumaki (real) 50 vol% ethanol extract) as a sample.

<Inhibition of neutral fat accumulation>
Subcutaneous fat-derived normal human preadipocytes Cryo HPRAD-SQ were seeded in a 96-well microplate so as to be 5.0 × 10 ³ cells per well. PGM medium (10% FBS, 2 mM L-glutamine, 100 units / mL Penicillin, containing 100 μg / mL Streptomycine) was used as the seeding medium. After culturing for 48 hours, the medium was replaced with a PGM differentiation medium (containing 10 μg / mL insulin, 1 μM Dexamethasone, 200 μM Indomethacin, 500 μM Isobutylmethylxanthine) to which a sample adjusted to an arbitrary concentration was added, and differentiation induction into adipocytes was performed. After initiation of differentiation induction, the cells were cultured for 10 to 14 days until the control group matured and many lipid droplets accumulated in the cells. After harvesting the cells, the cells were fixed using a 10% neutral buffered formaldehyde solution. After washing with PBS (−), 0.5 mass / volume% oil red O solution was added and cultured at 37 ° C. for 2 hours. After washing with PBS (−), methanol was added to extract the dye, and the absorbance at 550 nm was measured. At the same time, the absorbance at 650 nm was measured as turbidity, and the neutral fat accumulation inhibitory action was evaluated using the difference between the two measured values. The evaluation results are shown in Table 6 as relative values when the neutral fat accumulation amount in the control with no sample added is taken as 100.

  As is apparent from Table 6, the extract of the genus Makiaceae plants showed a significant effect of suppressing neutral fat accumulation in human preadipocytes. From this, it was clarified that the plant belonging to the genus Makiaceae has excellent neutral fat accumulation inhibitory action.

  The whitening effect of the extract of the genus Maki is shown. Evaluation was performed using Extract 1 (Inumakaki (leaf) hot water extract) as a sample.

<Inhibition of melanin production (whitening effect)>
The evaluation was performed according to the following procedure.
B16 mouse melanoma cells (B16F0 cells) were seeded in a 90 mm dish so that there were 18000 cells per dish. Dulbecco's modified Eagle medium (DMEM) supplemented with 5% by weight fetal bovine serum (FBS) was used as the seeding medium. After 24 hours, the medium was replaced with a 5% mass FBS-added DMEM medium to which the sample adjusted to each concentration was added, and further cultured for 5 days. After completion of the culture, cells were peeled off by trypsin treatment, transferred to a 1.5 mL microtube, and centrifuged to obtain a cell precipitate. The obtained precipitate was visually determined based on the following determination table. In the evaluation, a 5% mass FBS-added DMEM medium containing 5% mass FBS was used as a negative control, and a 5% mass FBS-added DMEM medium containing 50 mM sodium lactate was used as a positive control. These naked eye determination results were determined to be determination 5 and determination 1 and used as an index for sample determination. As shown in Table 7, the naked eye evaluation was evaluated in five stages. At the same time, the tissue solubilizer (trade name Solvable) was added to the precipitate, boiled, returned to room temperature, and the absorbance at 500 nm was measured with a spectrophotometer (HITACHI spectrophotometer U-3010) to determine the total amount of melanin. Asked. The evaluation results are shown in Table 8.

  As is clear from Table 8, it was found that the extract of the genus Makiaceae plant remarkably suppressed melanin production in the pigment cells. From this, it was clarified that the extract of the genus Makiaceae plant has an excellent inhibitory effect on melanin production and has a high whitening effect.

  The anti-inflammatory effect of the extract of the genus Maki is shown. As a sample, the evaluation was performed using the extract 2 (Inumaki (leaf) 50 vol% ethanol extract).

<Hyaluronidase inhibitory action (anti-inflammatory action)>
The evaluation was performed according to the following procedure.
Hyaluronic acid potassium salt (derived from human umbilical cord) was dissolved in 0.1 M phosphate buffer (pH 7.0) to a concentration of 0.9 mg / mL to obtain a substrate solution. Hyaluronidase (derived from bovine testis) was dissolved in 0.1 M phosphate buffer (pH 7.0) so as to be 5,300 units / mL to obtain an enzyme solution. A sample (0.1 mL) and an enzyme solution (0.03 mL) adjusted to each concentration with a buffer solution were placed in a test tube and reacted at 37 ° C. for 20 minutes. Next, 0.06 mL of an activator was added and reacted at 37 ° C. for 20 minutes. Further, 0.15 mL of the substrate solution was added and reacted at 37 ° C. for 1 hour. 0.4N NaOH (0.06 mL) was added, and the mixture was ice-cooled immediately after the reaction was stopped. A borate buffer (pH 9.1) was added (0.06 mL), boiled for 3 minutes, and further cooled with ice. After 2.0 mL of p-DABA solution was added and reacted at 37 ° C. for 20 minutes, the reaction solution was transferred to a 96-well microplate, and the absorbance at 585 nm was measured. As a control, a sample-free buffer solution was used. When the activity of hyaluronidase is inhibited, the degradation product N-acetylglucosamine (GlcNAc) is decreased, and the absorbance by p-DABA is decreased. Hyaluronidase inhibitory action is defined in equation (3).
Formula (3)
Inhibition rate (%) = (control absorbance−sample absorbance) / control absorbance × 100
Table 9 shows the evaluation results.

  As is clear from Table 9, the extract of the genus Makiaceae was found to have a hyaluronidase inhibitory action. From this, it was revealed that the extract of the genus Makiaceae plant has a hyaluronidase inhibitory action and has an excellent anti-inflammatory action.

  The formulation example which implemented this invention is shown.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (mass%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1% by weight aqueous solution) 20.0
(12) Extract 1 (Inumaki (leaf) hot water extract) 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification, cooling is started, and (11) and (12) are sequentially added and mixed uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (mass%)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Extract 2 (Inumaki (leaf) 50 vol% ethanol extract) 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (mass%)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20 mass% aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Extract 3 (Inumaki (fruit) 50 vol% ethanol extract) 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. (11) is added after completion | finish of emulsification, cooling is started, (12) is added at 40 degreeC, and it mixes uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (mass%)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1% by weight aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (derived from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Extract 1 (Inumaki (leaf) hot water extract) 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C., add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (mass%)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by mass aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Extract 2 (Inumaki (leaf) 50 vol% ethanol extract) 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 50.5 (mass%)
(2) 2-ethylhexane sancetyl 30.5
(3) Polyoxyethylene glyceryl isostearate 15.0
(4) Purified water 3.0
(5) Extract 1 (Inumaki (leaf) hot water extract) 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Formulation Example 7] Face-wash foam (1) Stearic acid 16.0 (mass%)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Extract 2 (Inumaki (leaf) 50 vol% ethanol extract) 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (mass%)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Extract 3 (Inumaki (real) 50 vol% ethanol extract) 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (mass%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Extract 1 (Inumaki (leaves) hot water extract) 1.2
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the water phase components (7) to (10) are mixed, dissolved by heating at 75 ° C., the pigments (11) to (15) are added thereto, and the mixture is uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by mass)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Extract 2 (Inumaki (leaf) 50 vol% ethanol extract) 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (mass%)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Extract 3 (Inumaki (fruit) 50 vol% ethanol extract) 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by mass)
(2) Extract 3 (Inumaki (fruit) 50 vol% ethanol extract) 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 15] Oral fluid (1) Extract 2 (Inumaki (leaf) 50 vol% ethanol extract) 8.0 (mass%)
(2) Erythritol 1.0
(3) Citric acid 0.1
(4) Stevia 0.01
(5) Purified water 90.89
Production method: (1) to (5) are mixed uniformly.

[Prescription Example 16] Granule (1) Extract 1 (Inumaki (leaves) hot water extract) 0.2 (parts by mass)
(2) Lactose 0.65
(3) Corn starch 0.15
Production method: (1) to (3) were passed and mixed, granulated with a granulator, dried and sized to obtain granules having a total amount of 1500 mg.

Claims (1)

Neutral fat accumulation inhibitor containing an extract of Udonaki fruit .