JP5158352B2 - Antiviral agent - Google Patents

Description

  The present invention relates to an ethanol-containing antiviral agent having a virus inactivating effect.

  Alcohol preparations based on alcohol are widely used as disinfectants because they can be used directly in foods and can be used in cooking utensils, tableware, kitchen spaces, and the like. On the other hand, norovirus causing acute gastroenteritis has been on the rise in recent years and is becoming the mainstream of recent food poisoning and infectious diseases. However, the conventional alcohol preparation has a problem that its effect on Norovirus (by a substitute experiment with feline calicivirus) is insufficient. From the above, an alcohol preparation capable of dealing with norovirus has been particularly desired.

  In addition, the alcohol preparation is required to have little influence on food because it is used directly on food, and it is desired that the composition has excellent stability. Furthermore, since the subject to which the alcohol preparation is used includes a metal cooking utensil or the like, an alcohol preparation capable of preventing metal corrosion has been desired.

Japanese Patent Publication No.58-2668 JP-B-2-31946

  The present invention has been made in view of the above circumstances, has an inactivating effect on viruses, particularly norovirus (by a substitute experiment with feline calicivirus), has little influence on food, prevents metal corrosion, An object is to provide an antiviral agent having excellent stability.

  As a result of intensive studies to achieve the above object, the present inventors have formulated (A) ethanol, (B) organic acid salt, and (C) eucalyptus extract in specific amounts, respectively. In particular, it has been found that it has an inactivating effect on norovirus (by alternative experiment with feline calicivirus), has little influence on food, prevents corrosion of metal, and has excellent stability of the composition. It has come.

Therefore, the present invention comprises (A) ethanol 40-60% by mass, (B) organic acid salt 0.05-0.5% by mass, (C) eucalyptus extract 0.05-0.5% by mass, And an antiviral agent against norovirus or feline calicivirus having a pH (15 ° C.) of 8-9 .

  ADVANTAGE OF THE INVENTION According to this invention, the anti-virus which has the inactivation effect of viruses, especially Norovirus (by the alternative experiment by a feline calicivirus), has little influence on food, prevents metal corrosion, and has excellent composition stability. An agent can be provided.

  The antiviral agent of the present invention comprises (A) ethanol 40 to 60% by mass, (B) organic acid salt 0.05 to 0.5% by mass, and (C) eucalyptus extract 0.05 to 0.5% by mass. %.

(A) Ethanol Examples of ethanol include fermented ethanol, synthetic ethanol, and the like, and any of them can be used alone or in combination of two or more. Ethanol has bactericidal performance, and its blending amount is 40 to 60% by mass with respect to the total amount of the antiviral agent. Usually, a high-concentration ethanol solution of 70 to 80% by mass is considered to have high bactericidal performance. However, it is particularly effective to inactivate norovirus by setting the blending amount to 40 to 60% by mass, preferably 45 to 58% by mass. Becomes higher. On the other hand, when the blending amount is less than 40% by mass, the virus inactivating effect is insufficient, and when it exceeds 60% by mass, the stability (25 ° C.) is deteriorated.

(B) Organic acid salt By adding an organic acid salt, the virus inactivating effect can be further improved, and there is no concern about corrosion of metallic cookware. Examples of the organic acid salt include citrate, lactate, malate, tartrate, oxalate, acetate, formate, succinate, fumarate, gluconate salt and the like. It can use individually or in combination of 2 or more types as appropriate. Examples of the salt include alkali metal salts and the like, and may be hydrates. Among these, citrate is preferable, and trisodium citrate dihydrate is more preferable.

  (B) The compounding quantity of a component is 0.05-0.5 mass% with respect to antiviral agent whole quantity, and 0.1-0.3 mass% is preferable. When the blending amount is less than 0.05% by mass, the effect of component (B) blending cannot be obtained, and when it exceeds 0.5% by mass, the stability is deteriorated and crystals are precipitated.

(C) Eucalyptus extract Eucalyptus extract refers to an extract from a plant belonging to the genus Eucalyptus. As a eucalyptus extract, what was obtained by the well-known extraction method, a commercial item, etc. can be used. The extraction site is not particularly limited, but leaves are preferred. The extraction method is not particularly limited, and a known extraction method such as solvent extraction, steam distillation, or supercritical extraction can be employed. For example, in the case of solvent extraction, it can be obtained by cutting and processing eucalyptus raw or after drying to a suitable size, and immersing and stirring in the extraction solvent. The extraction may be heated as necessary, the extraction time is appropriately selected, and the extraction pH is not particularly limited unless it is extremely acidic or alkaline. Examples of the solvent used in the extraction method include water; alcohols such as methanol, ethanol, propanol, and butanol; polyhydric alcohols such as propylene glycol and butylene glycol, and the like. They can be used in appropriate combinations. After extraction, filtration may be performed to appropriately perform known purification.

  The shape of the eucalyptus extract is not particularly limited, and examples thereof include those obtained by the above-described treatment as they are, such as liquids such as dilutions and concentrates, pastes, and dry powders by freeze drying.

  (C) The compounding quantity of a component is 0.05-0.5 mass% with respect to antiviral agent whole quantity, and 0.1-0.4 mass% is preferable. When the blending amount exceeds 0.5% by mass, when used in food, there is an adverse effect such as causing bitterness, and the color of the antiviral agent becomes deep.

  In the antiviral agent of the present invention, an appropriate amount can be used alone or in combination of two or more of the components to be blended in the alcohol preparation within a range not impairing the effects of the present invention. Examples of such components include preservatives, antioxidants, pH adjusters, surfactants, pigments, fragrances, and water.

  The antiviral agent of the present invention can be prepared, for example, by mixing the above components (A) to (C), optional components and water (remainder) as necessary, based on a conventional method.

  The pH (15 ° C.) of the antiviral agent of the present invention is preferably 8-9. If the pH is less than 8, the virus inactivating effect may be lowered, and it is desirable that the pH does not exceed 9 from the viewpoint of preventing the stability of the eucalyptus extract and metal corrosion.

  The antiviral agent of the present invention is particularly effective as a norovirus (by alternative experiment with feline calicivirus) inactivation effect, and is suitable as an antiviral agent against norovirus or feline calicivirus.

  The antiviral agent of the present invention is food, skin such as hands, kitchen utensils, cooking utensils such as pans, dishes such as dishes, kitchen countertops, sinks (triangular corners), kitchen floors, kitchen spaces such as walls, etc. It can be applied to refrigerators, toilets, baths, toilets, and other areas where there is a risk of virus attachment.

  The method of using the antiviral agent of the present invention is not particularly limited. For example, the object is immersed in the antiviral agent, and the antiviral agent is applied to or sprayed on the object (for example, trigger spray, dispenser spray, aerosol, etc.). It can be naturally dried as it is, or it can be used after being air-dried or wiped off. The amount to be applied or sprayed onto the object is not particularly limited as long as it can be applied or sprayed evenly onto the object, and the amount is about 1 to 10 mL.

  EXAMPLES Hereinafter, although an Example and a comparative example are shown and this invention is demonstrated concretely, this invention is not restrict | limited to the following Example. In the following examples, unless otherwise specified, “%” of the composition is mass%, and the amount of each component in the table is a pure amount.

[Examples 1-7, Comparative Examples 1-6]
Trisodium citrate dihydrate, ethanol, and eucalyptus extract were sequentially added to purified water, and finally the amount was adjusted with purified water to obtain antiviral agents having the compositions shown in Tables 2 and 3. The following evaluation was performed about the obtained antiviral agent. The results are also shown in the table.

[Virus inactivation]
It investigated using the feline calicivirus. Since norovirus culture systems have not been established, a method for confirming virus infectivity using feline calicivirus belonging to the same caliciidae as a substitute virus for norovirus is generally employed. Feline calicivirus is a pathogen of feline infectious disease called feline calicivirus infection, which is similar in structure to norovirus and has similar properties.

The cells were infected with virus, and the virus infection titer was determined based on the 50% cultured cell infection concentration (TCID ₅₀ ) by the limiting dilution method.
Test virus: Feline calicivirus, ATCC VR-782
Test cells: CRFK cells: Cat kidney cells (CRFK: Crandell-Reese Feline Kidney), ATCC CCL-94

In a test tube, 900 μL of a sample was inoculated with 100 μL of feline calicivirus solution, mixed well by vortexing, and allowed to act at room temperature for 60 seconds (stock solution). Then, this mixed solution was diluted and neutralized 10 times with PBS (-): Phospate-Buffered Saline, product name: Dulbecco's phosphate buffer (Invitrogen). Furthermore, in order to determine the virus infectivity titer, serial dilution was performed 10 times with PBS (-).
CRFK cells monolayer cultured in 96-well plates using 10% FCS (+) MEM were inoculated with 25 μL of the diluted product in 4 wells, respectively, and feline calicivirus and CRFK cells were contacted. Furthermore, 100 μL of 0.2% MEM FCS (+) was overlaid and cultured in a CO ₂ gas furan vessel at 37 ° C. for 3 to 5 days.
Observation was carried out under a microscope, and the dilution factor at which cytopathy caused by the virus was observed in half of the four wells (50%) was determined. The logarithm of the dilution factor was used as the virus infectivity value.
Blank was performed in the same manner except that a PBS (−) solution was used instead of the sample, and a virus having a virus infectivity of 4 was used. The decrease value of the virus infectivity value of the sample with respect to the blank (blank infectivity value-virus infectivity value of the sample) was determined. Based on the decreased value of the virus infectivity, the virus inactivation effect was shown by the following evaluation criteria.
<Evaluation criteria>
A: Decreased value of virus infection value 4
○: Decrease value of virus infection value 3
Δ: Decrease value of virus infection value 2-1
×: Reduced value of virus infection value 0
The 10% FCS (+) MEM composition is shown below.

[Metal corrosion prevention]
0.5 mL of the sample was dropped onto an aluminum plate (A1100P, thickness × width × length = 1.0 × 25 × 75 mm), and the surface was wiped off after 24 hours. The presence or absence of corrosion on the metal surface was compared with a metal plate not dripped with the sample and confirmed visually. A result is shown by the following evaluation criteria.
<Evaluation criteria>
○: Not corroded ×: Corroded

[Impact on food]
About 1.2 mL of the sample was sprayed with a sprayer on reddish sashimi (tuna). After leaving for 2 hours in the refrigerator, the effect on taste when eating sashimi as it was was evaluated.
<Evaluation criteria>
○: No change △: Slightly affected ×: Influenced (Up to ○ and △, it is judged that there is no problem even if used for food)

[Stability]
The sample (50 mL) was filled in a 50 mL vial and stored at room temperature for 30 days. After storage, the appearance was evaluated based on the following evaluation criteria.
<Evaluation criteria>
○: Uniform and transparent ×: Crystal precipitation

The raw materials used in the above examples are shown below.
Ethanol: Fermented ethanol traceable 95 grade 1 (manufactured by Nippon Alcohol Sales Co., Ltd.) 10 (flavor H-No. 13) (manufactured by Hasegawa Fragrance Co., Ltd.) is added at a rate of 1 kg / 200 L. The alcohol active ingredient was 91.88%, and it was blended in terms of pure content.
Trisodium citrate dihydrate: Trisodium citrate (food additive grade) (manufactured by Junsei Co., Ltd.)
Eucalyptus extract: Eucalyptus extract, 50% ethanol extract (Oji Wood Greening Co., Ltd.)
Purified water: manufactured by Seiki Pharmaceutical Co., Ltd.

Claims (3)

(A) ethanol 40-60 mass%, (B) organic acid salt 0.05-0.5 mass%, and (C) eucalyptus extract 0.05-0.5 mass%, pH ( An antiviral agent against norovirus or feline calicivirus, wherein 15 ° C) is 8-9 . The antibacterial composition according to claim 1, wherein the component (B) is selected from citrate, lactate, malate, tartrate, oxalate, acetate, formate, succinate, fumarate and glucosan salt. Viral agent. The antiviral agent according to claim 2, wherein the component (B) is citrate.