JP4815798B2 - Copper-containing composition for oral administration - Google Patents

Description

  The present invention relates to a composition for oral administration in which an unpleasant taste of a copper compound is reduced and a feeling of taking is improved.

  The copper-containing composition for oral administration was difficult to drink due to an unpleasant taste derived from copper ions. This taste is the same as the taste when using an astringent that binds to proteins such as tannin and alum (astringent taste). Until now, in order to improve the unpleasant taste of a copper compound, the technique of mix | blending an oleoresin and an accent fragrance | flavor is disclosed (patent document 1). However, these are not sufficient in improving the unpleasant taste derived from copper ions.

Japanese Patent Laid-Open No. 01-153622

  An object of the present invention is to provide a copper-containing composition for oral administration that reduces the astringent taste derived from copper ions, is easy to drink, and does not leave an unpleasant aftertaste.

  As a result of intensive studies in order to solve the above problems, the present inventors have found that when a collagen peptide and a vegetable peptide are blended in a composition for oral administration containing a copper compound, protein aggregation (convergence) is caused by copper ions. ) Is reduced and the astringency is improved, and the present invention has been completed. That is, this invention is a composition for oral administration which mix | blended the peptide with the copper compound.

  According to the present invention, it is possible to provide a copper-containing composition for oral administration that reduces the astringent taste derived from copper ions, is easy to drink, and does not leave an unpleasant aftertaste.

  The copper compound in the present invention is a salt containing copper, and if the anion is non-toxic, either an organic ion or an inorganic ion may be used. Examples of the organic ion include organic acids such as gluconic acid, citric acid, and tartaric acid. Examples of the ions and inorganic ions include inorganic ions such as sulfuric acid, hydrochloric acid, nitric acid, phosphoric acid, and carbonic acid. Preferred copper compounds include copper gluconate, copper sulfate, copper citrate, copper chloride, copper nitrate and copper phosphate. These may be blended singly or in combination of two or more.

  The compounding amount of the copper compound varies depending on the purpose of use, but in terms of nutrient intake, 0.1 to 10 mg per day is preferable in terms of copper ions. In addition, when ingesting as a 100 ml drink a day, the copper ion concentration is 0.0001 to 0.01 W / V%.

  The peptide in the present invention is a protein that is decomposed with an enzyme or an acid to improve the function of the protein or add a new function. There are functional ones. Among such protein degradation products, collagen peptides derived from livestock meat or fish meat, and plant peptides derived from corn are particularly preferred.

  In the composition for oral administration of the present invention, the compounding ratio of copper ions and peptides is usually 0.1 parts by weight or more, preferably 0.5 to 30000 parts by weight, based on 1 part by weight of copper ions. Preferably it is 2.5-4000 weight part.

  When the copper-containing composition for oral administration of the present invention is used as an internal solution, the pH is adjusted to 2.5 to 7.0, preferably 3.0 to 5.5 in consideration of antiseptic properties and flavor. . For example, citric acid, malic acid, fumaric acid, tartaric acid, lactic acid, succinic acid, and other organic acids and salts thereof, phosphoric acid, hydrochloric acid, and other inorganic acids, and inorganic bases such as sodium hydroxide are added. Can be adjusted.

  In the copper-containing composition for oral administration of the present invention, vitamins, minerals, amino acids, herbal medicines, herbal extracts, caffeine, royal jelly and the like can be appropriately blended within a range not impairing the effects of the present invention. Moreover, additives such as an antioxidant, a colorant, a fragrance, a corrigent, a surfactant, a solubilizing agent, a preservative, and a sweetener can be appropriately blended as necessary so long as the effects of the present invention are not impaired.

  The method for preparing the copper-containing composition for oral administration of the present invention is not particularly limited. When using it as an internal solution, the purpose is usually to dissolve each component with an appropriate amount of purified water, adjust the pH, adjust the volume by adding the remaining purified water, and filter and sterilize as necessary. A copper-containing composition for oral administration is obtained.

  Hereinafter, the present invention will be described in more detail with reference to examples and test examples.

Example 1
Copper gluconate 0.004g
Zinc gluconate 0.02g
Corn peptide 1.00g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.01 g
0.01 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Aminoethylsulfonic acid 2.00g
Xylitol 4.00g
Trehalose 5.00g
Erythritol 5.00g
Citric acid 0.80 g
Sodium citrate appropriate amount Sodium benzoate 0.06g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.0, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 2
Copper gluconate 0.007g
Zinc gluconate 0.05g
Collagen peptide 1.00g
Calcium gluconate 0.80 g
0.01 g of thiamine nitrate
Riboflavin 0.01g
0.10 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Aminoethylsulfonic acid 1.00g
Sorbitol 4.00g
Trehalose 5.00g
Xylitol 4.00g
Stevia extract 0.03g
Acesulfame potassium 0.03g
Malic acid 0.10g
Citric acid 0.40g
Sodium citrate appropriate amount Benzoic acid 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
Apple flavor 0.10g
After dissolving the above components in purified water, the pH was adjusted to 4.0, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 3
0.03 g of copper gluconate
Zinc gluconate 0.08g
Corn peptide 0.50g
Riboflavin 0.01g
0.01 g of pyridoxine hydrochloride
Ascorbic acid 1.00g
Cyanocobalamin 120μg
Panthenol 0.01g
Nicotinamide 0.05g
Aminoethylsulfonic acid 1.00g
Sorbitol 5.00g
Trehalose 2.00g
Maltitol 2.00g
Citric acid 0.40g
Sodium malate appropriate amount Benzoic acid 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 4.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 4
Copper gluconate 0.007g
Zinc gluconate 0.05g
Collagen peptide 0.30g
Calcium gluconate 0.80 g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.01 g
0.01 g of pyridoxine hydrochloride
Nicotinamide 0.10g
Anhydrous caffeine 0.10g
Aminoethylsulfonic acid 2.00g
Yokuinin style extract 2.00mL
Glucose 5.00g
Indigestible dextrin 4.00 g
Erythritol 5.00g
Xylitol 2.00g
Stevia extract 0.02g
Acesulfame potassium 0.03g
Sucralose 0.05g
Citric acid 0.80 g
Sodium citrate appropriate amount Sodium benzoate 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Example 5
0.03 g of copper gluconate
Zinc gluconate 0.08g
Collagen peptide 0.30g
Calcium gluconate 0.40g
Calcium lactate 0.50g
0.01 g of thiamine nitrate
Riboflavin sodium phosphate 0.02g
0.03 g of pyridoxine hydrochloride
Nicotinamide 0.05g
Anhydrous caffeine 0.10g
Aminoethylsulfonic acid 2.00g
Yokuinin style extract 2.00mL
Royal jelly 0.60g
Glucose 5.00g
Sorbitol 5.00g
Xylitol 5.00g
Stevia extract 0.02g
Acesulfame potassium 0.03g
Citric acid 0.80 g
Sodium citrate 0.10g
Phosphoric acid 0.30 g
Hydrochloric acid appropriate amount Sodium benzoate 0.06g
Butyl paraoxybenzoate 0.006g
Propyl paraoxybenzoate 0.006 g
0.10g mixed fruit flavor
After dissolving the above components in purified water, the pH was adjusted to 3.5, and purified water was added to make up a total volume of 100 mL. This solution was filtered with a filter paper, and the filtrate was sterilized by heating at 80 ° C. for 25 minutes using a sterilizer, and then filled into a glass bottle and capped to obtain an internal solution.

Test example
Hagerman et al. Reported that protein (bovine serum albumin: BSA) in solution was aggregated by tannin, and the amount of precipitation was proportional to the amount of tannin ( J. Agric . Food . Chem ., 1978, Vol. 26 , 809) . -812). The present inventors have found that when a BSA solution is added to a copper ion solution, the solution is suspended, the amount of light transmission decreases, and the decrease in the amount of transmission correlates with the concentration of copper ions.

Test method (1) Preparation of BSA solution: 6 g of BSA (Sigma Chemical Co .; fraction V, fatty acid free) is dissolved in an appropriate amount of purified water, 100 mg of citric acid is added, and the pH is adjusted with NaOH solution (1 mol / L). Adjusted to 4.8 and made up to 100 mL with purified water.

  (2) Preparation of diluting solution: 100 mg of citric acid was dissolved in an appropriate amount of water, pH was adjusted to 4.8 with NaOH solution (1 mol / L), and purified water was adjusted to 100 mL.

  (3) Preparation of copper ion solution: 0.10 g of citric acid was added to 0.02 g, 0.11 g and 0.18 g of copper gluconate. Each was dissolved in an appropriate amount of purified water, adjusted to pH 4.8 with NaOH solution (1 mol / L), and made up to 100 mL with purified water.

(4) Evaluation of protein-copper ion interaction (convergence) 6 mL of BSA solution was added to 2 mL of each copper ion solution, and the total volume was adjusted to 10 mL with a diluted solution. This was shaken at 40 ° C. for 30 minutes. Using a quartz cell (L = 1 cm), the absorbance at 500 nm, which is a wavelength that is not absorbed by each transparent solution, was measured with a spectrophotometer (manufactured by Hitachi, Ltd .: U-3300). This result (FIG. 1) shows that the copper ion concentration and the absorbance are correlated.

  Next, it was confirmed that the results of sensory evaluation of the astringent taste of various concentrations of the copper ion solution correlated with the absorbance when the BSA solution was added to the copper ion solution. The sensory evaluation was performed as B when the astringent taste was strongly unacceptable, A4 to A1 according to the strength of the astringent taste, and A when the astringent taste was not felt at all.

  The results are shown in FIG. When the absorbance was about 0.3 or less, it was felt that the astringent taste was sufficiently suppressed. From this, if the absorbance when a copper ion solution added with a certain substance is mixed with a BSA solution is decreased as compared with the case where no addition is made, the addition of that substance causes aggregation due to protein-copper ion interaction ( This means that the (convergence) has decreased, that is, the astringency has decreased.

(5) Protein aggregation properties of specimens Protein aggregation properties of the formulations shown in Table 1 were evaluated by the methods described in (1) to (4) above. The results are also shown in Table 1.

  From Table 1, it can be seen that the absorbance of Sample 1 and Sample 2 containing appropriate amounts of corn peptide and collagen peptide is smaller than the corresponding Sample 3. From this result, it became clear that the astringent taste derived from copper ions can be reduced by blending corn peptide and collagen peptide.

The correlation between protein aggregation and copper ion concentration is shown. The correlation between the suspension (protein aggregation) generated when a BSA solution is added to a copper ion solution and the result of sensory evaluation of the copper ion solution is shown.

The copper-containing composition for oral administration of the present invention can be applied to, for example, various preparations including pharmaceuticals such as syrups and drinks and quasi drugs and various beverages such as nutritional functional foods.

Claims (3)

One or two or more copper compounds selected from the group consisting of copper gluconate, copper sulfate, copper citrate, copper chloride, copper nitrate and copper phosphate, and one or two selected from collagen peptides and corn peptides A composition for oral administration, wherein the composition is an internal preparation prepared by dissolving pH of the peptide in purified water, adjusting the pH, and adding the remaining purified water . The composition for oral administration according to claim 1, wherein the copper compound is copper gluconate. The composition for oral administration according to any one of claims 1 and 2 , which is an internal solution having a pH of 2.5 to 7.0.

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