JP4726135B2 - Fluid handling equipment - Google Patents

Description

  The present invention forms a liquid-liquid interface in the communication part between the flow paths and the flow path, and by measuring a plurality of such communication parts, a trace amount of liquid is measured between the communication parts, or the measured liquid The present invention relates to a fluid handling device for performing electrophoresis.

  Conventionally, a minute amount of liquid containing an analyte such as protein and nucleic acid in a living body is measured in the flow path, and the minute amount of liquid is electrophoresed in the flow path to flow the analyte in the liquid. A fluid handling device has been developed that is configured to be detected and analyzed by a measuring device disposed in the middle of the road.

(First conventional example)
FIG. 12 shows a first conventional example of such a fluid handling apparatus. The conventional fluid handling apparatus 100 shown in FIG. 12 is formed of PDMS (polydimethylsiloxane) which is a material having high gas permeability, and is a liquid sample containing an analysis object (protein, nucleic acid, DNA, etc.). And a second flow channel 102 as a sample introduction channel connected so as to be orthogonal to the middle of the first flow channel 101. Ports 103, 104, and 105 are formed at both ends of the first flow path 101 and the end of the second flow path 102, respectively. In addition, the first flow path 101 of the fluid handling apparatus 100 is formed with a pair of stop valves 106a and 106b that rapidly squeeze the cross-sectional area of the flow path to block the liquid, and between the stop valves 106a and 106b, A second flow path 102 is connected in the vicinity of the stop valve 106a on the upstream side in the migration direction (left side in FIG. 12) (see FIG. 12A).

  The fluid handling device 100 configured as described above is housed in the vacuum device 107, whereby the gas in the liquid handling device 100 including the gas in the first flow path 101 and the second flow path 102 is degassed. After the first and second flow paths 101 and 102 are in a vacuum state (see FIG. 12B), a liquid sample containing DNA in the port 105 located at the end of the second flow path 102 When 110 is dropped and the polymer solutions 111 and 112 are dropped into the ports 103 and 104 at both ends of the first channel 101 (see FIG. 12C), the sample 110 passes through the second channel 102. A negative pressure is sucked (introduced) into the first flow path 101 between the pair of stop valves 106a and 106b, and the polymer solution 111 is sucked into the first flow path 101 between the port 103 and the stop valve 106a with a negative pressure. (Introduced Furthermore, the polymer solution 112 is sucked into the first flow path 101 between the port 104 and the stop valve 106b with a negative pressure, and the first flow path 101 is filled with the polymer solutions 111 and 112 and the sample 110. (See FIG. 12D).

  Here, the fluid handling apparatus 100 can measure the sample 110 by a desired amount if the flow volume between the pair of stop valves 106a and 106b of the first flow path 101 is set to a desired size. it can. Then, electrodes are arranged in the ports 103 and 104 at both ends of the first flow channel 101 and the port 105 at the end of the second flow channel 102, and the liquid (polymer) in the first flow channel 101 and the second flow channel 102 is arranged. A voltage is applied to the solutions 111 and 112 and the sample 110) to return the analyte in the second channel 102 to the port 105 side (see FIGS. 12E and 12F), and in the first channel 101 The measurement object located between the stop valve 106b and the port 104 is electrophoresed in the right direction (D direction) of the first flow path 101 in the figure beyond the right stop valve 106b in the figure. Only a predetermined amount of the object to be analyzed between the stop valves 106a and 106b can be accurately analyzed by the apparatus (see Non-Patent Document 1).

(Second conventional example)
FIG. 13 shows a second conventional example of the fluid handling apparatus. The fluid handling apparatus 200 according to the second conventional example shown in FIG. 13A has a flow path C and a flow path D that connect the flow path A and the flow path B arranged in parallel. , C is connected with a degassing flow path E in a flow path D whose flow cross-sectional area is rapidly reduced. Among these channels A to E, the channel D is a channel wall surface that is difficult to wet (capillary repulsion is likely to occur), and the liquid cannot move in the channel D due to capillary action. On the other hand, the channel A and the channel C are channel walls that are easily wetted (capillary phenomenon is likely to occur), and the channel cross-sectional area of the channel C is smaller than the channel cross-sectional area of the channel A. Yes.

  As a result, when the liquid sample 201 is introduced into the flow path A of the fluid handling apparatus 200, the sample in the flow path A is drawn into the flow path C by capillary action, but flows into the flow path C. The sample 201 is blocked by the flow path D, and a predetermined amount of the sample 201 is measured in the flow path C (see FIG. 13B). In addition, since the polymer solution 202 for causing the analyte in the sample 201 to be electrophoresed is filled in the channel B, if the gas is sealed in the channel C, the sample in the channel A 201 cannot be introduced into the flow path C by capillary action. Therefore, when the sample 201 of the flow path A is introduced into the flow path C, the flow path E is opened, and the gas in the flow path C is released to the outside through the flow path E.

  In such a state, the first stage pressure (gas pressure) is applied to the flow channel A so that the sample 201 of the flow channel C does not flow out from the flow channel D to the flow channel B side. Is moved to the downstream side (right side in the figure) from the connection with the channel C, and a desired amount of the sample 201 is measured in the channel C (see FIG. 13C). Thereafter, the second stage pressure is higher than the first stage pressure in the channel A and the channel C and the sample 201 in the channel C passes through the channel D and flows out to the channel B side. (Gas pressure) is applied. As a result, the sample 201 in the channel C moves into the channel B via the channel D (see FIG. 13D). Note that when the sample 201 in the channel C is moved to the channel B side via the channel D, the degassing channel E is closed.

A voltage is applied to both ends of the flow path B, and the analyte of the sample 201 introduced into the flow path B from the flow path C through the flow path D is electrophoresed (Patent Document 1).
(Third conventional example)
In addition, in the analysis of samples such as proteins and nucleic acids, a fluid handling device (electrophoresis chip) capable of accurately measuring and quantitatively analyzing a very small amount of sample required for analysis has been developed. In such a fluid handling device, by using a gas control device in combination so that no gas remains in the flow channel, the sample piece that contacts the buffer solution and the liquid-liquid interface is placed in the flow channel for electrophoresis. Can be formed. (Patent Document 2)
μTAS2004, 8th International Conference on Miniaturized Systems for Chemistry and Life Sciences JP 2004-163104 A JP 2005-114433 A

  However, since the fluid handling apparatus 100 of the first conventional example needs to degas the gas in the flow path (first and second flow paths 101 and 102) in the vacuum apparatus 107, the apparatus becomes large. In addition, there is a problem that the pre-treatment time (preparation work time until the sample analysis work is started) takes a long time.

  Further, the fluid handling apparatus 200 of the second conventional example closes the flow path E when moving the sample 201 in the flow path C to the flow path B via the flow path D. The gas remaining in D may enter the flow path B together with the sample 201, which may impair the smooth electrophoresis of the sample 201. In addition, the fluid handling apparatus 200 of the second conventional example needs to apply two-stage pressure to the flow path A, and the operation becomes complicated, and a pressurizing means must be connected to the flow path A. This complicates the structure of the apparatus and increases the size of the entire structure including the pressurizing means.

  Further, in the fluid handling device of the third conventional example, in order to degas the gas in the device, positive / negative pressure operations by the gas control device are required, so that the operation becomes complicated and the gas control device The structure of the entire apparatus including the above is complicated and has the same problem as that of the second conventional example, which increases the size.

  Therefore, the present invention has a simple and compact structure, does not contain gas, and does not require an intentional pressure operation from the outside. An object of the present invention is to provide a fluid handling device capable of achieving the above.

  The fluid handling device according to the first aspect of the present invention includes a first main channel through which a first fluid can move by capillary action, a second main channel through which a second fluid can move by capillary action, and a capillary phenomenon. A third main channel through which a third fluid can move, a first communication unit that communicates the first main channel, the second main channel, and the external environment, the first main channel, A third main flow path and a second communication part for communicating the external environment. Among these, the first connecting part is (1) a first sub-flow path that is formed so that the first fluid can move by capillary action, and connects the first main flow path and the external environment. (2) a second sub-flow path formed so that the second fluid can move by capillary action and connects the second main flow path and the external environment; and (3) the first main flow path. And a cross-sectional area smaller than the cross-sectional area of the second main flow path, and the first fluid or the second fluid is movably formed by capillary action, and the first main flow path and A third sub-flow channel communicating with the second main flow channel. In addition, the second communication part is (1) a fourth sub-channel that is formed so that the first fluid can move by capillary action and communicates the first main channel and the external environment; (2) a fifth sub-channel formed so that the third fluid can move by capillary action, and communicates the third main channel and the external environment; and (3) the first main channel A cross-sectional area that is smaller than a cross-sectional area of the third main flow path, and the first fluid or the third fluid is movably formed by capillary action; And a sixth sub-flow channel communicating with the third main flow channel. Further, the present invention provides the first fluid that moves in the first main channel toward the first connecting portion and the second fluid that moves in the second main channel toward the first connecting portion. Form an interface in the first communication part, and move toward the second communication part toward the second communication part and the first fluid moving toward the second communication part. The third fluid moving in the third main flow path forms an interface at the second connecting portion. The present invention is characterized in that the first fluid is measured between the first connecting portion and the second connecting portion.

  A fluid handling apparatus according to a second aspect of the present invention includes a first main channel through which a first fluid can move by capillary action, a second main channel through which a second fluid can move by capillary action, and a capillary phenomenon. A third main channel through which a third fluid can move, a first communication unit that communicates the first main channel, the second main channel, and the external environment, the first main channel, A third main flow path and a second communication part for communicating the external environment. Among these, the first connecting part is (1) a first sub-flow path that is formed so that the first fluid can move by capillary action, and connects the first main flow path and the external environment. (2) a second sub-flow path formed so that the second fluid can move by capillary action and connects the second main flow path and the external environment; and (3) the first main flow path. And a cross-sectional area smaller than the cross-sectional area of the second main flow path, the first fluid and / or the second fluid are movably formed by capillary action, and the first main flow And a third sub-flow path that communicates the path, the second main flow path, and the external environment. In addition, the second communication part is (1) a fourth sub-channel that is formed so that the first fluid can move by capillary action and communicates the first main channel and the external environment; (2) a fifth sub-channel formed so that the third fluid can move by capillary action, and communicates the third main channel and the external environment; and (3) the first main channel A cross-sectional area that is smaller than a cross-sectional area of the third main flow path, and the first fluid or the third fluid is movably formed by capillary action; And a sixth sub-flow channel communicating with the third main flow channel. Further, the present invention provides the first fluid that moves in the first main channel toward the first connecting portion and the second fluid that moves in the second main channel toward the first connecting portion. Form an interface in the first communication part, and move toward the second communication part toward the second communication part and the first fluid moving toward the second communication part. The third fluid moving in the third main flow path forms an interface at the second connecting portion. The present invention is characterized in that the first fluid is measured between the first connecting portion and the second connecting portion.

  According to a third aspect of the present invention, there is provided a fluid handling apparatus including: a first main channel through which a first fluid can move by capillary action; a second main channel through which a second fluid can move by capillary action; and a capillary phenomenon. A third main channel through which a third fluid can move, a first communication unit that communicates the first main channel, the second main channel, and the external environment, the first main channel, A third main flow path and a second communication part for communicating the external environment. Among these, the first connecting part is (1) a first sub-flow path that is formed so that the first fluid can move by capillary action, and connects the first main flow path and the external environment. (2) a second sub-flow path formed so that the second fluid can move by capillary action and connects the second main flow path and the external environment; and (3) the first main flow path. And a cross-sectional area smaller than the cross-sectional area of the second main flow path, the first fluid and / or the second fluid are movably formed by capillary action, and the first main flow And a third sub-flow path that communicates the path, the second main flow path, and the external environment. In addition, the second communication part is (1) a fourth sub-channel that is formed so that the first fluid can move by capillary action and communicates the first main channel and the external environment; (2) a fifth sub-channel formed so that the third fluid can move by capillary action, and communicates the third main channel and the external environment; and (3) the first main channel The first main flow path has a cross-sectional area smaller than a cross-sectional area of the third main flow path, and the first fluid and / or the third fluid is movably formed by capillary action. And a sixth sub-channel that communicates the third main channel and the external environment. Further, the present invention provides the first fluid that moves in the first main channel toward the first connecting portion and the second fluid that moves in the second main channel toward the first connecting portion. Form an interface in the first communication part, and move toward the second communication part toward the second communication part and the first fluid moving toward the second communication part. The third fluid moving in the third main flow path forms an interface at the second connecting portion. The present invention is characterized in that the first fluid is measured between the first connecting portion and the second connecting portion.

  According to a fourth aspect of the present invention, in the fluid handling apparatus according to any one of the first to third aspects, the first fluid is introduced into the first main flow path in the first main flow path. A first port is formed, a second port for introducing the second fluid into the second main channel is formed in the second main channel, and the third main channel is provided in the third main channel. A third port for introducing the third fluid into the third main flow path is formed.

  According to a fifth aspect of the present invention, in the fluid handling apparatus according to the fourth aspect of the invention, the first port is formed in the vicinity of the first communication portion or the second communication portion. .

The invention of claim 6 is the fluid handling device according to claim 4 or 5, wherein the first port is connected to the first main flow path via a first fluid introduction path,
The first fluid is formed so as to be able to flow from the first fluid introduction path side to the first main flow path side by capillary action.

  According to a seventh aspect of the present invention, in the fluid handling device according to the fourth or fifth aspect, the first port is connected to the first main flow path via a first fluid introduction path. The first main flow path side connection portion of the first fluid introduction path is formed with a cross-sectional area smaller than the flow path cross-sectional area of the main part of the first fluid introduction path and the first main flow path. The first fluid is formed so as to be able to flow from the first fluid introduction path side to the first main flow path side by capillary action.

  The invention of claim 8 is the fluid handling device according to any one of claims 1 to 7, further comprising a potential difference applying means for applying a potential difference between the second main channel and the third main channel, The charged substance contained in the first fluid in the first main flow path is electrophoresed to the second main flow path or the third main flow path.

  According to the fluid handling device of the present invention, the first fluid introduced into the first main channel and the second fluid introduced into the second main channel form a liquid / liquid interface in the first communication portion. The first fluid introduced into the first main flow path and the third fluid introduced into the third main flow path form a liquid / liquid interface in the second connecting portion, and are intentionally applied from the outside. Only the first fluid in the first main flow path can be easily measured by a desired amount in accordance with the volume of the first main flow path without requiring any pressure operation. Therefore, according to the present invention, there is no need for a vacuum device as in the first conventional example, and no pressure operating means as in the second to third conventional examples, the structure is simplified, and the overall structure is reduced in size. can do. Further, according to the present invention, there is no need for a pretreatment operation for evacuation by a vacuum apparatus as in the first conventional example, and the pressure in the flow path is controlled by pressure operating means as in the second to third conventional examples. Since the control operation is not necessary, the analysis work time of the fluid by electrophoresis can be remarkably shortened.

  In the fluid handling device of the present invention, when the first fluid is introduced into the first main flow path and the first fluid reaches the first connecting portion, the gas in the first main flow path is changed to the first main flow path. The gas is pushed by one fluid, and the gas is released to the external environment through the first sub-flow channel, and is externally supplied through the third sub-flow channel, the second main flow channel, and the second sub-flow channel. Released into the environment. When the first fluid introduced into the first main channel reaches the second connecting portion, the gas in the first main channel is pushed by the first fluid, and the gas flows into the fourth substream. In addition to being released to the external environment via the path, it is released to the external environment via the sixth sub-flow path, the third main flow path, and the fifth sub-flow path. After that, when the second fluid is introduced into the second main channel, the gas in the second main channel is pushed by the second fluid that moves by capillary action, and passes through the second sub-channel. To the outside environment. As a result, the second fluid in the second main flow channel and the first fluid in the third sub flow channel form a liquid-liquid interface in the first communication portion without any gas remaining. Can do. Further, when the third fluid is introduced into the third main channel, the gas in the third main channel is pushed by the third fluid that moves by capillary action, and passes through the fifth sub-channel. To the outside environment. As a result, the third fluid in the third main flow channel and the first fluid in the sixth sub flow channel form a liquid / liquid interface in the second communication portion without any gas remaining. Can do. Therefore, according to the present invention, a potential difference is generated between the second main flow path and the third main flow path, so that the first fluid in the first main flow path is transferred to the second main flow path or the third main flow path. When electrophoresing in the main flow path, electrophoretic failure due to residual gas bubbles does not occur, and the analysis of the first fluid by electrophoresis can be performed smoothly and accurately.

  In the second and third aspects of the invention, the third sub-flow path in the first connecting portion communicates the first main flow path, the second main flow path, and the external environment. Therefore, even if the first fluid and the second fluid reach the first communication portion at the same time, the gas pushed by the first fluid and the second fluid can be released to the external environment. The fluid and the second fluid can be caused to flow by capillary action to form a liquid / liquid interface therein.

  Further, in the invention of claim 3, since the sixth sub-flow path in the second connecting portion communicates the first main flow path, the third main flow path, and the external environment, Even if the first fluid and the third fluid reach the second connecting portion at the same time, the gas pushed by the first fluid and the third fluid can be released to the external environment. And the third fluid can be caused to flow by capillary action to form a liquid-liquid interface therein.

  Further, in the first aspect of the invention, the first fluid and the second fluid at the first main flow path side end or the second main flow path side end of the third sub flow path in the first connecting portion A liquid interface can be formed.

  Further, in the first and second aspects of the invention, the first fluid and the third fluid are mixed at the first main flow path side end or the third main flow path side end of the sixth sub flow path in the second connecting portion. A liquid-liquid interface can be formed.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

[First Embodiment]
FIG. 1 shows a fluid handling apparatus 1 according to a first embodiment of the present invention. In FIG. 1, (a) is a plan view of the fluid handling device 1, (b) is a side view of the fluid handling device 1, and (c) is cut along line AA in (a). FIG. As shown in FIG. 1, the fluid handling apparatus 1 of the present embodiment is superposed so as to cover the thin plate-like first member 2 whose planar shape is a rectangular shape and the entire back surface 3 of the first member 2. It consists of a thin plate-like second member 4. The first member 2 and the second member 4 are formed using various resin materials such as PMMA (polymethyl methacrylate), PC (polycarbonate) and ultraviolet curable resin, glass, ceramics, and the like. The first member 2 and the second member 4 are formed so that the overlapping surfaces (the back surface 3 of the first member 2 and the surface 5 of the second member 4 (see FIG. 2)) are smooth surfaces with good adhesion. In a state where the overlapping surfaces are in close contact with each other, they are unseparable or integrated so as to be separable by adhesive fixing, fastening fixing, clip fixing, or the like. In addition, in this embodiment, although the 1st member 2 and the 2nd member 4 illustrated the thin plate-shaped thing, it is not restricted to this, The block-shaped thing which is a cube may be sufficient. Moreover, the 2nd member 4 superimposed on the back surface 3 side of the 1st member 2 may be a film-like thing.

  FIG. 2A is a rear view of the first member 2. 2A, on the back surface 3 side of the first member 2, a first groove 6 extending linearly in the lateral direction on the back surface 3 side, and one end side of the first groove 6 are provided. A second groove 8 communicating with the first connecting portion 7 via the first connecting portion 7, a third groove 11 communicating with the other end of the first groove 6 via the second connecting portion 10, and a first groove A first external environment communication groove 12 communicating with the first groove 6 and the second groove 8 via the communication part 7, and a first groove 6 and a third groove 11 via the second communication part 10. A second external environment communication groove 13 communicating with the first groove 6 and a sample introduction groove 14 communicating with the first groove 6 are formed. The first groove 6, the second groove 8, the third groove 11, the first and second external environment communication grooves 12 and 13, and the sample introduction groove 14 are all rectangular in cross section. And opened to the back surface 3 side of the first member 2. A hole 15 penetrating the front and back of the first member 2 is formed at the end of the sample introduction groove 14. Further, the end portions of the second groove 8, the third groove 11, and the first and second external environment communication grooves 12, 13 have holes 16, 17, 18, penetrating through the front and back of the first member 2, respectively. 20 is formed.

  FIG. 3 shows details of the first communication unit 7 and the second communication unit 10. As shown in FIG. 3, the first connecting portion 7 has the first groove cross-sectional area smaller than the groove cross-sectional area of the first groove 6, the second groove 8, and the first external environment communication groove 12. A sub-groove 21, a second sub-groove 22, and a third sub-groove 23 are formed. Among these, the first sub-groove 21 communicates the first groove 6 and the first external environment communication groove 12. The second sub-groove 22 communicates the second groove 8 and the first external environment communication groove 12. Further, the third sub-groove 23 communicates the first groove 6, the second groove 8, and the first external environment communication groove 12 with each other. Further, the second connecting portion 10 includes fourth sub-grooves 24, 5 having a groove cross-sectional area smaller than the groove cross-sectional areas of the first groove 6, the third groove 11, and the second external environment communication groove 13. The sub-groove 25 and the sixth sub-groove 26 are formed. Among these, the fourth sub-groove 24 communicates the first groove 6 and the second external environment communication groove 13. The fifth sub-groove 25 communicates the third groove 11 and the second external environment communication groove 13. The sixth sub-groove 26 communicates the first groove 6, the third groove 11, and the second external environment communication groove 13 with each other. And these 1st-6th subgrooves 21-26 are the cross-sectional shape of a groove | channel, and are opened to the back surface 3 side of the 1st member 2. As shown in FIG.

  As shown in FIGS. 1 and 3, the second member 4 is superimposed on the back surface 3 of the first member 2 as described above, and the first groove 6, the second groove 8, the third groove 11, the first By closing the opening on the back surface 3 side of the second external environment communication grooves 12 and 13, the sample introduction groove 14, the first to sixth sub-grooves 21 to 26, and the holes 15 to 18 and 20 with the second member 4. , First main flow path 27, second main flow path 28, third main flow path 30, first to second external environment communication paths 31, 32, sample introduction path (first fluid introduction path) 33, first The 1st-6th subchannels 41-46, the 1st-3rd ports 47-49, and the 1st-2nd external environment communication ports 50 and 51 are formed.

  That is, as shown in FIG. 3, the first main flow path 27 communicates with the second main flow path 28 via the first connection portion 7, and the third main flow path 27 via the second connection portion 10. The main channel 30 communicates with the main channel 30. The first main flow path 27 communicates with the first external environment communication path 31 via the first communication part 7 and the second external environment communication path 32 via the second communication part 10. It is communicated to.

  In addition, as shown in FIG. 3, in the first connecting portion 7, the first sub-channel 41 communicates the first main channel 27 and the first external environment communication channel 31, and the second sub-channel 42 communicates the second main flow path 28 and the first external environment communication path 31, and the third sub-flow path 43 serves as the first main flow path 27, the second main flow path 28, and the first external environment communication path. The passages 31 communicate with each other.

  In addition, as shown in FIG. 3, in the second communication unit 10, the fourth sub-flow path 44 communicates the first main flow path 27 and the second external environment communication path 32, and the fifth sub-flow path 45 communicates the third main flow path 30 and the second external environment communication path 32, and the sixth sub-flow path 46 serves as the first main flow path 27, the third main flow path 30 and the second external environment communication path. 32 communicate with each other.

  Here, the above-described first to third main flow paths 27, 28 and 30, the sample introduction path 33, and the first to sixth sub-flow paths 41 to 46 are liquids introduced into the flow path due to capillary action. In view of the affinity between the flow path and the liquid (for example, in the case of a liquid having a large surface tension, the flow path surface characteristic is made lyophilic). .

  As shown in FIG. 4, the fluid handling apparatus 1 configured as described above introduces a sample (hereinafter referred to as “sample”) 52 containing an analysis target as a first fluid into the first port 47. Then, the sample 52 is introduced into the first main channel 27 through the sample introduction channel 33 by capillary action. At this time, the gas in the first main channel 27 (including the gas in the sample introduction channel 33) is pushed by the sample 52 flowing in the first main channel 27 by capillary action, The first sub-flow channel 41 and the first external environment communication path 31 are used to deaerate the external environment, and the third sub-flow channel 43, the second main flow channel 28, the second sub-flow channel 42, and The air is deaerated to the external environment via the first external environment communication path 31. In addition, the sample 52 flowing through the sample introduction path 33 pushes gas to the second connecting portion 10 side, and is deaerated to the external environment via the fourth sub-flow path 44 and the second external environment communication path 32. At the same time, the air is deaerated to the external environment via the sixth sub-flow path 46, the third main flow path 30, the fifth sub-flow path 45, and the second external environment communication path 32.

  As a result, as shown in FIG. 4, when the sample 52 that flows through the first main flow path 27 by capillary action reaches the first connecting portion 7, the sample 52 pushes out the gas in the first sub flow path 41. However, it flows by capillary action to the first external environment communication passage 31 side end portion of the first sub-flow channel 41. Further, the sample 52 flows by capillarity from the end of the third sub-channel 43 toward the second main channel 28 and the end of the external environment communication channel 31 while extruding gas through the third sub-channel 43. To do. The sample 52 that has flowed to the end of the first sub-flow channel 41 and the third sub-flow channel 43 on the first external environment communication path 31 side by the capillary phenomenon is the first sub-flow channel 41 and the third sub-flow. Since the flow path suddenly expands from the path 43 to the first external environment communication path 31, it stops at the first external environment communication path 31 side opening end of the first sub flow path 41 and the third sub flow path 43. To do. In addition, the sample 52 that has flowed by capillary action to the end of the third sub-channel 43 toward the second main channel 28 is rapidly expanded from the third sub-channel 43 to the second main channel 28. Therefore, the third sub-flow path 43 stops at the opening end on the second main flow path 28 side.

  As shown in FIG. 4, when the sample flowing in the first main flow path 27 by capillary action reaches the second connecting portion 10, the sample 52 pushes the gas in the fourth sub flow path 44 while pushing out the gas. It flows by capillary action up to the second external environment communication path 32 side end of the fourth sub-flow path 44. Further, the sample 52 flows by capillarity from the end of the sixth sub-channel 46 toward the third main channel 30 and the end of the external environment communication channel 32 while extruding gas through the sixth sub-channel 46. To do. The sample 52 that has flowed by capillarity to the end of the fourth sub-channel 44 and the sixth sub-channel 46 on the second external environment communication channel 32 side is the fourth sub-channel 44 and the sixth sub-stream. Since the flow path suddenly expands from the path 46 to the second external environment communication path 32, it stops at the opening end of the fourth sub flow path 44 and the sixth sub flow path 46 on the second external environment communication path 32 side. To do. In addition, the sample 52 that has flowed to the end of the sixth sub-channel 46 toward the third main channel 30 side by capillarity rapidly expands from the sixth sub-channel 46 to the third main channel 30. Therefore, the sixth sub-flow path 46 stops at the opening end on the third main flow path 30 side.

  Next, as shown in FIG. 5, when polymer solutions (second and third fluids) 53 and 54 are injected from the second port 48 and the third port 49, respectively, the polymer solution injected from the second port 48 The (second fluid) 53 flows in the second main flow path 28 by capillary action, and the polymer solution (third fluid) 54 injected from the third port 49 flows in the third main flow path 30 in the capillary action. Fluid. At this time, when the polymer solution 53 injected from the second port 48 flows through the second main flow path 28 by capillary action, gas is passed from the second sub-flow path 42 through the first external environment communication path 31. And then reaches the first connecting portion 7, and a liquid / liquid interface between the polymer solution 53 and the sample 52 is formed at the boundary between the second main flow path 28 and the third sub flow path 43. At the same time, the second sub-flow channel 42 flows to the end portion on the first external environment communication path 31 side by capillary action. The polymer solution that has flowed by capillary action to the end of the second sub-channel 42 on the first external environment communication channel 31 side is disconnected from the second sub-channel 42 to the first external environment communication channel 31. Since the area suddenly expands, the second sub-flow path 42 stops at the opening end on the first external environment communication path 31 side. Further, the polymer solution 54 injected from the third port 49 causes gas to flow from the fifth sub-channel 45 via the second external environment communication channel 32 when flowing through the third main channel 30 by capillary action. The liquid is discharged to the external environment, reaches the second connecting portion 10, and a liquid / liquid interface between the polymer solution 54 and the sample 52 is formed at the boundary between the third main flow path 30 and the sixth sub flow path 46. At the same time, the fluid flows to the end of the fifth sub-channel 45 on the second external environment communication path 32 side by capillary action. The polymer solution 54 that has flowed to the second external environment communication passage 32 side end portion of the fifth sub flow channel 45 by the capillary phenomenon flows from the fifth sub flow channel 45 to the second external environment communication passage 32. Since the cross-sectional area suddenly expands, it stops at the opening end on the second external environment communication path 32 side of the fifth sub-channel 45. In the present embodiment, the external environment communication paths 31 and 32 are formed. However, the external environment communication paths 31 and 32 are not formed, and the first, second, fourth, and fifth substreams are formed. One end of the paths 41, 42, 44, 45 may be directly communicated with the external environment.

  As described above, according to the present embodiment, no gas remains in the first to third main flow paths 27, 28, 30 and the first to second communication parts 7, 10, and the sample 52 or polymer solution 53 and 54 are filled, and the liquid / liquid interface is reliably formed in the first connecting portion 7 and the second connecting portion 10 (see FIG. 5).

  Further, in the fluid handling device 1 of the present embodiment, the volume of the first main flow path 27 located between the first connecting portion 7 and the second connecting portion 10 is set to a desired volume, and this first volume is set. The main flow path 27 is a measurement flow path section for measuring a desired amount of sample (see FIGS. 3 to 5). Note that the volume of the first main flow path 27 as the measurement flow path section is V1, and the flow volume of the third sub flow path 43 in the first communication section 7 and the sixth volume in the second communication section 10 are the same. When the channel volume of the sub channel 46 is V2, V2 is extremely smaller than V1 (V1 >> 2.V2). Therefore, the sample 52 is accurately measured by the first main flow path 27.

  After that, electrodes (not shown) are arranged in the second port 48 of the second main channel 28, the third port 49 of the third main channel 30, and the first port 47 of the sample introduction channel 33, and the first main stream A voltage is applied to the sample 52 in the channel 27 and the sample introduction channel 33, the polymer solution 53 in the second main channel 28, and the polymer solution 54 in the third main channel 30 by the potential difference applying means, and the first main channel The sample 52 in the sample 27 and the sample 52 in the sample introduction channel 33 are electrophoresed. As a result, in the sample 52 in the first main channel 27, a difference occurs in the electrophoresis speed due to the molecular weight or the like of the contained analyte, and various bands are generated. The various bands are passed through the first connecting section 7. Electrophoresis is performed on the second main flow path 28 side, and the sample measurement means (not shown) arranged in the second main flow path 28 is used to measure / analyze the object to be analyzed, or various bands thereof are used as the second band. The analyte can be measured / analyzed by a sample measuring means (not shown) that is electrophoresed to the third main channel 30 side via the communication unit 10 and arranged in the third main channel 30. Further, the analysis object of the sample 52 in the sample introduction path 33 is electrophoresed and returned to the first port 47 side (see FIG. 5).

  When the electrophoresis is performed in this way, the formation position of the sample introduction path 33 is the end of the first main flow path 27 for measuring the sample 52 and the upstream position in the migration direction. desirable. By forming in this way, the analyte in the sample 52 measured in the first main channel 27 is prevented from returning to the sample introduction channel 33 by electrophoresis, and quantitative analysis is further performed. It becomes possible to carry out accurately.

  The fluid handling device 1 of the present embodiment having the above-described structure can introduce the liquid sample 52 into the first main flow path 27 via the sample introduction path 33 by capillary action, and the first communication unit 7. A desired amount of the sample 52 can be easily measured by the first main channel 27 located between the first communication channel 10 and the second communication unit 10.

  Further, the fluid handling apparatus 1 of the present embodiment can introduce the liquid sample 52 into the first main channel 27 via the sample introduction channel 33 by capillary action, and is used for causing the sample 52 to undergo electrophoresis. The polymer solutions 53 and 54 to be supplied can be introduced into the second main channel 28 and the third main channel 30 from the second port 48 and the third port 49 by capillary action, Since the sample 52 and the polymer solutions 53 and 54 can be brought into contact with each other at the second connecting portion 10 to form a liquid / liquid interface, a vacuum device as in the first conventional example is not necessary, and the second conventional example is provided. Such a pressurizing means is not required, the structure is simplified, and the entire structure can be reduced in size.

  Further, the fluid handling apparatus 1 of the present embodiment can introduce the liquid sample 52 into the first main channel 27 via the sample introduction channel 33 by capillary action, and is used for causing the sample 52 to undergo electrophoresis. The polymer solutions 53 and 54 to be supplied can be introduced into the second main channel 28 and the third main channel 30 from the second port 48 and the third port 49 by capillarity, and the first communication unit 7 and Since the sample 52 and the polymer solutions 53 and 54 can be brought into contact with each other at the second connecting portion 10, there is no need for a pretreatment operation for evacuation by a vacuum device as in the first conventional example. Since it is not necessary to pressurize the flow path in two stages by such pressurizing means, the analysis work time of the sample 52 can be remarkably shortened.

  In addition, the fluid handling device 1 of the present embodiment allows the gas in the first main flow path 27 (including the gas in the sample introduction path 33) and the gas in the second main flow path 28 to pass through the first communication unit 7. The gas in the first main flow path 27 (including the gas in the sample introduction path 33) and the gas in the third main flow path 30 can be discharged to the external environment via the second connecting unit 10. Since it can be released, the sample 52 and the polymer solutions 53 and 54 can form a liquid-liquid interface (interface where no gas intervenes), and no gas is mixed into the sample 52 and the polymer solutions 53 and 54. The operation of electrophoresis and analysis of the sample 52 in the first main channel 27 is performed smoothly and accurately.

  Note that in the fluid handling device 1 of the present embodiment, when the sample 52 and the polymer solution 53 reach the first communication unit 7 at the same time, the gas in the third subflow channel 43 flows into the third subflow. The sample 52 and the polymer solution 53 flowing in the channel 43 by capillary action are pushed out to the first external environment communication path 31 side, and the liquid / liquid interface between the sample 52 and the polymer solution 53 in the third sub-flow channel 43. It is formed. In addition, when the sample 52 and the polymer solution 54 reach the second connecting portion 10 at the same time, the sample in which the gas in the sixth subchannel 46 flows in the sixth subchannel 46 by capillary action. 52 and the polymer solution 54 are pushed out toward the second external environment communication path 32, and a liquid / liquid interface between the sample 52 and the polymer solution 54 is formed in the sixth sub-flow path 46.

  In order to make the degassing more reliable in such a case, the width of the branch flow path 43a to the first external environment communication path 31 of the third sub flow path 43, the sixth sub flow path 46, and the like. The width of the branch flow path 46a to the second external environment communication path 32, the flow length of the third sub flow path 43 (the length from the first main flow path 27 to the second main flow path 28), and The sixth sub-flow channel 46 may be expanded to the extent of the flow length (the length from the first main flow channel 27 to the third main flow channel 30). The branch flow paths 43a and 46a are referred to as the side surface 1b side (see FIG. 1) where the first and second sub flow paths 41 and 42 and the fourth and fifth sub flow paths 44 and 45 are formed. If it is formed on the opposite side surface 1a side (see FIG. 1), it can be formed with the same width as the flow length of the third sub-channel 43 and the sixth sub-channel 46 (FIG. 5 (e)). )reference.).

  As in this embodiment, the widths of the first to sixth sub-channels 41 to 46 in the first connecting section 7 and the second connecting section 10 are narrowed in the plate thickness direction (first to sixth sub-grooves). The first to third main flow paths 27, 28, 30 and the first main flow paths 27, 28, 30 and the first flow path can be widened by increasing the width in the plate thickness direction and the direction perpendicular to the fluid flow direction. The cross-sectional areas of the first to sixth sub-flow paths 41 to 46 can be made smaller than those of the second external environment communication paths 31 and 32, and the gas in the flow path can be surely degassed. .

  When the aspect ratio of the first to sixth sub-grooves 21 to 26 forming the first to sixth sub-flow channels 41 to 46 is large, that is, when the ratio of the groove depth to the groove width is large. In the injection molding, it is difficult to transfer the shape of the groove bottom, and it is difficult to obtain a desired shape. On the other hand, by reducing the aspect ratio of the first to sixth sub-grooves 21 to 26 as in the present embodiment, that is, by increasing the groove width with respect to the groove depth, the desired cross-sectional area is obtained. The first to sixth sub-grooves 21 to 26 having a desired shape can be easily formed by injection molding.

[Second Embodiment]
FIG. 6 shows a fluid handling apparatus 1 according to the second embodiment of the present invention. In the fluid handling device 1 of the present embodiment, the same components as those of the fluid handling device 1 of the first embodiment are denoted by the same reference numerals, and redundant description is omitted.

  In the fluid handling apparatus 1 shown in FIG. 6, the magnetic beads 55 to which the analysis object in the sample 52 adheres are mixed in the sample 52, and the sample 52 including the magnetic beads 55 is introduced into the first port 47 for sample introduction. To the first connecting portion 7 and the second connecting portion 10 through the sample introduction path 33 and the first flow path 27 by capillary action, and the polymer solution 53 dripped into the second port 48 is added to the second port 48. The polymer solution 54 is introduced to the end of the flow path 28 on the first connecting portion 7 side by capillary action, and the polymer solution 54 dropped on the third port 49 is guided to the second connecting section 10 of the third flow path 30 by capillary action. It is supposed to be put in. At this time, the gas positioned between the sample 52 and the polymer solution 53 is externally passed through the first to third sub-flow paths 41 to 43 of the first communication unit 7 and the first external environment communication path 31. Released into the environment. In addition, the gas positioned between the sample 52 and the polymer solution 54 passes through the fourth to sixth sub-flow paths 44 to 46 and the second external environment communication path 32 of the second communication unit 10 to the external environment. To be released. As a result, the sample 52 and the polymer solutions 53 and 54 can form a liquid / liquid interface in the first connecting portion 7 and the second connecting portion 10 without interposing a gas.

  Thereafter, the fluid handling apparatus 1 collects the magnetic beads 55 on one end side (the left end portion in FIG. 6A) of the first main flow path 27 by the magnet (bead collecting means) 56 and adheres to the magnetic beads 55. The density of the analyzed object in the sample 52 is increased.

  Next, the fluid handling apparatus 1 separates the analyte captured by the magnetic beads 55 from the magnetic beads 55 by using the collected magnetic beads 55 as light as separation means, or a free liquid, and the like. By analyzing the separated and concentrated analysis target object from the first main flow path 27 to the second main flow path 28, the analysis target object in the sample 52 is analyzed.

  According to such a fluid handling device 1 of the present embodiment, it is possible to obtain the same function and effect as those of the first embodiment. Therefore, it is possible to accurately measure and analyze the analysis object.

  In the present embodiment, the position where the magnet 56 is disposed is not limited to one end side of the first main flow path 27, and is appropriately changed as long as the analysis object in the sample 52 can be efficiently concentrated. be able to.

  Further, in the present embodiment, the first connecting part 7 side end and the first connecting part 7 of the first main channel 27 or the second connecting part 10 side end of the first main channel 27 and the first connecting part 7 side. The magnetic beads 55 introduced up to the second communication part 10 are in the state before the polymer solutions 53 and 54 are introduced into the second main flow path 28 and the third main flow path 30. 7 and the second connecting portion 10, and the sample 52 flows out to the second main flow path 28, the third main flow path 30, the first external environment communication path 31, and the second external environment communication path 32 side. Therefore, the gas is trapped at the gas / sample gas / liquid interface in the first communication unit 7 or the gas / sample gas / liquid interface in the second communication unit 10. Thus, even if the second main flow path 28 and the third main flow path 30 are filled with the polymer solutions 53 and 54 after the first main flow path 27 is filled, and the electrophoresis is performed, the magnetic beads 55 remain in the second state. Since the speed of diffusion toward the main flow path 28 is extremely slow compared to the migration of the analyte, it does not hinder accurate analysis.

[Third Embodiment]
FIG. 7 shows a fluid handling apparatus 1 according to the third embodiment of the present invention. In the fluid handling device 1 of the present embodiment, the same components as those of the fluid handling device 1 of the first embodiment are denoted by the same reference numerals, and redundant description is omitted.

  The fluid handling apparatus 1 according to this embodiment employs first and second connecting portions 7 and 10 having a different structure from the first and second connecting portions 7 and 10 of the first and second embodiments described above. A mode is shown.

  That is, as shown in FIG. 7, the first and second connecting portions 7 and 10 in the fluid handling device 1 according to this embodiment are the first and second embodiments shown in the first and second embodiments. Whereas the first to sixth sub-channels 41 to 46 of the connecting portions 7 and 10 are designed to narrow the channel width in the thickness direction of the first member 2, the thickness direction of the first member 2 Without narrowing the flow path width, the flow path width is narrowed in the width direction of the first member 2 (in the plan view, the direction orthogonal to the direction in which the first main flow path 27 extends).

  In the fluid handling device 1 of this embodiment having such a configuration, the first and second connecting portions 7 and 10 are the same as the first and second connecting portions 7 and 10 of the first and second embodiments described above. Similar liquid reservoir effect (when a fluid flows from a channel with a small cross-sectional area toward a channel with a rapidly expanded cross-sectional area, the channel with a cross-sectional area with a small cross-sectional area is rapidly expanded. The effect that the fluid stops at the open end of the.

[Fourth Embodiment]
FIG. 8 shows a fluid handling apparatus 1 according to the fourth embodiment of the present invention. The fluid handling device 1 of the present embodiment is a modification of the above-described third embodiment. The same components as those of the fluid handling device 1 of the above-described third embodiment are denoted by the same reference numerals, and redundant description is given. Is omitted.

  The fluid handling apparatus according to this embodiment is different from the third embodiment described above in the third sub-flow path 43 in the first communication section 7 and the sixth sub-flow path 46 in the second communication section 10.

  In other words, in the present embodiment, the third sub-channel 43 communicates the first main channel 27 and the second main channel 28. However, the third sub-flow path 43 is similar to the third sub-flow path 43 of the third embodiment described above, and the first main flow path 27, the second main flow path 28, and the first external environment communication path. 31 do not communicate with each other. Further, the sixth sub-flow path 46 communicates the first main flow path 27 and the third main flow path 30. However, the sixth sub-channel 46 is similar to the sixth sub-channel 46 of the third embodiment described above, and the first main channel 27, the third main channel 30, and the second external environment communication channel. 32 do not communicate with each other.

  Therefore, in this embodiment, after the sample 52 is introduced into the first main channel 27, the polymer solutions 53 and 54 are introduced into the second main channel 28 and the third main channel 30, or the second After introducing the polymer solutions 53 and 54 into the main channel 28 and the third main channel 30, the sample 52 is introduced into the first main channel 27, etc. After the sixth sub-channel 46 of the second communication unit 10 is filled with either the sample 52 or the polymer solutions 53 and 54, the other of the sample 52 and the polymer solutions 53 and 54 is the first communication unit 7. It is necessary to reach the second communication unit 10.

  In this embodiment, when the sample 52 introduced into the first main channel 27 and the polymer solution 53 introduced into the second main channel 28 reach the first connecting portion 7 at the same time, the third sub-channel 43 is provided. In this case, gas is interposed between the sample 52 and the polymer solution 53, and it becomes difficult to form a liquid / liquid interface in the first connecting portion 7. In addition, when the sample 52 introduced into the first main channel 27 and the polymer solution 54 introduced into the third main channel 30 reach the second connecting portion 10 at the same time, the sample 52 is contained inside the sixth sub-channel 46. Since a gas is interposed between the liquid and the polymer solution 54, it is difficult to form a liquid / liquid interface in the second connecting portion 10. Therefore, in this embodiment, a time difference is provided in the time for the sample 52 and the polymer solution 53 to reach the first connecting portion 7, and the time for the sample 52 and the polymer solution 54 to reach the second connecting portion 10. The timing for injecting the sample 52 into the first port 47 and the timing for injecting the polymer solutions 53 and 54 into the second port 48 or the third port 49 are determined so as to provide a time difference.

  The fluid handling device 1 of this embodiment used in such a manner seems to provide a time difference for filling the first to third main flow paths 27, 28, 30 with the sample 52 and the polymer solutions 53, 54. However, it is possible to obtain the same operations and effects as the above-described embodiments.

[Fifth Embodiment]
9 to 10 show a fluid handling apparatus 1 according to a fifth embodiment of the present invention. The fluid handling device 1 of the present embodiment is a modification of the first embodiment described above, and the same components as those of the fluid handling device 1 of the first embodiment described above are denoted by the same reference numerals and redundant description is given. Is omitted.

  The fluid handling device 1 of this embodiment is characterized by a sample introduction path (first fluid introduction path) 33 that connects (communications) the first port 47 and the first main flow path 27. That is, the sample introduction path 33 of the fluid handling device 1 of the present embodiment is formed by the main part 33b of the sample introduction path 33 and the first main flow path side connection part 33a. The first main channel side connecting portion 33a formed to have a channel cross-sectional area smaller than one main channel 27 is connected (communication) to the first main channel 27 at an inclination angle of 45 °. Therefore, the sample (first fluid) in the main portion 33b of the sample introduction path 33 can flow into the first main flow channel 26 by the capillary action via the first main flow channel side connection portion 33a. .

  According to this embodiment having such a configuration, a voltage is applied to the sample in the first main channel 27 and the sample introduction channel 33, the polymer solution in the second main channel 28, and the polymer solution in the third main channel 30. When a voltage is applied by the applying means to cause electrophoresis of the sample in the first main flow path 27 and the analyte in the sample introduction path 33, the sample introduction path 33 (first main flow path side connection portion 33a). The analyte in the vicinity of the opening on the first main channel 27 side (portion indicated by the hatched portion 60a in FIG. 11A) flows toward the inside of the first main channel 27 or in the sample introduction channel 33. It will flow toward. At this time, the analysis object (shaded part 60a) in the vicinity of the opening on the first main channel 27 side of the sample introduction path 33 flows toward either the first main channel 27 side or the sample introduction path 33 side. As a result, the amount of the analyte to be electrophoresed in the first main channel 27 is increased or decreased.

  However, according to the configuration of the present embodiment, the first main flow path side connection portion 33a of the sample introduction path 33 is smaller in flow path cross-sectional area than the main section 33b of the sample introduction path 33 and the first main flow path 27. Because of the cross-sectional area (see FIG. 11 (a)), as shown in FIG. 11 (b), the sample introduction path 33 and the first main flow path 27 are formed so as to have the same cross-sectional area over the entire length. Compared with the configuration of the first embodiment in which the two are orthogonally connected to each other, the analysis object in the vicinity of the opening on the first main channel 27 side of the sample introduction channel 33 (shaded portion 60a in FIG. 11A). Is much smaller than the amount of the analysis object (shaded portion 60b in FIG. 11B) in the vicinity of the opening on the first main channel 27 side of the sample introduction path 33 in the first embodiment.

  Therefore, in the fluid handling device 1 of the present embodiment, the variation in the amount of the analyte to be electrophoresed in the first main flow path 27 is smaller than that of the fluid handling device 1 of the first embodiment, so that a more accurate sample can be obtained. Measurement and more accurate analysis are possible.

  In addition, the structure of this embodiment can be applied not only to the fluid handling apparatus 1 of the first embodiment but also to the fluid handling apparatus 1 of the second to fourth embodiments.

  In the fluid handling apparatus 1 according to the present embodiment, the entire sample introduction path 33 (the entire area including the vicinity of the first main channel side opening) is the first main channel as shown in FIG. 27 is formed by inclining by an inclination angle θ (= 45 °) in a clockwise direction from a direction orthogonal to the direction (+ Y direction in FIG. 11A), but is not limited thereto, and from a flow path having a small cross-sectional area. If the effect of stopping the fluid at the opening end to the channel where the cross-sectional area is abruptly expanded is not exerted, and if it is inclined at an angle that allows capillary attraction to work from a channel with a small cross-sectional area to a large channel, Good.

  That is, in the present embodiment, the angle (inclination angle θ) at which the first main channel side connecting portion 33a of the sample introduction channel 33 is inclined with respect to the direction orthogonal to the first main channel 27 is the sample, the channel wall surface, and the like. Is set to an angle at which the sample can flow from the sample introduction path 33 side into the first main flow path 27 only by capillary action, and is not limited to θ = 45 °.

  Further, in the fluid handling apparatus 1 according to the present embodiment, the entire sample introduction path 33 is orthogonal to the first main flow path 27, as shown in FIGS. 9 (a), 10 (a), and 11 (a). However, the present invention is not limited to this, and only the first main channel side connecting portion 33a of the sample introduction channel 33 is orthogonal to the first main channel 27. Alternatively, the connection may be made with an inclination of the inclination angle θ.

[Other Embodiments]
In the present invention, the first communication unit 7 and the second communication unit 10 are respectively connected to the first communication unit 7 of the first embodiment, the third embodiment, and the fourth embodiment. You may use the thing of the structure which combined the part 10 suitably. That is, in the fluid handling device 1 of the present invention, the structure of the first connecting part 7 is the structure of the first connecting part 7 of any of the first, third, and fourth embodiments, and the structure of the second connecting part 10 is used. It is good also as a structure of the 2nd connection part 10 in any one of 1st, 3rd, 4th embodiment.

  The first and second connecting portions 7 and 10 are not limited to those of the first to fourth embodiments (7, 10) described above, and flow in the thickness direction and the width direction of the first member 2. The road may be narrowed down. Even in this case, the first and second connecting portions 7 and 10 have the cross-sectional areas of the first to sixth sub-channels 41 to 46 as the first to third main channels 27, 28, and 30. In addition, since the flow path cross-sectional area of the first and second external environment communication passages 31 and 32 can be made smaller than the first and second external environment communication passages 31 and 32, the sample 52 that flows due to capillary action in the first and second communication portions 7 and 10. Can be dammed up.

  In each of the above-described embodiments, the first to third grooves 6, 8, 11, the first to sixth sub-grooves 21 to 26, the first and second external environment communication grooves 12, 13 are provided as the first. Although it forms in the back surface 3 side of the member 2, it is not restricted to this, You may form in the surface 5 side of the 2nd member 4, and the back surface 3 side and 2nd member of the 1st member 2 4 may be formed across the front surface 5 side.

It is a figure which shows the fluid handling apparatus which concerns on 1st Embodiment of this invention, FIG.1 (a) is a top view of a fluid handling apparatus, FIG.1 (b) is a side view of a fluid handling apparatus, FIG.1 (c) is FIG. It is sectional drawing cut | disconnected and shown along the AA line of Fig.1 (a). 2A is a back view of the first member, and FIG. 2B is a plan view of the second member. 3A is a view cut along the line BB in FIG. 1B, and FIG. 3B is an enlarged view of the vicinity of the connecting portion 32 in FIG. 3A. 3C is a cross-sectional view taken along line CC in FIG. 1A, and FIG. 3D is an enlarged view of the vicinity of the connecting portion 32 in FIG. 3C. It is sectional drawing shown. It is a figure which shows the 1st operation state of the fluid handling apparatus which concerns on 1st Embodiment, and Fig.4 (a)-(d) is a figure corresponding to Fig.3 (a)-(d). It is a figure which shows the 2nd operation state of the fluid handling apparatus which concerns on 1st Embodiment, and Fig.5 (a)-(d) is a figure corresponding to Fig.3 (a)-(d). It is a figure which shows the fluid handling apparatus which concerns on 2nd Embodiment of this invention, and Fig.6 (a)-(d) is a figure corresponding to Fig.5 (a)-(d). It is a figure which shows the fluid handling apparatus which concerns on 3rd Embodiment of this invention, and Fig.7 (a), (b) is a figure corresponding to Fig.5 (a), (b). It is a figure which shows the fluid handling apparatus which concerns on 4th Embodiment of this invention, FIG. 8 (a), (b) is a figure corresponding to FIG. 5 (a), (b), Comprising: FIG.8 (c) These are figures corresponding to Fig.6 (a). It is a figure which shows the fluid handling apparatus which concerns on 5th Embodiment of this invention, Fig.9 (a) is a top view of a fluid handling apparatus, FIG.9 (b) is a side view of the fluid handling apparatus shown to Fig.9 (a). FIG. 9C is a cross-sectional view of the fluid handling device shown cut along the line A1-A1 in FIG. 9A. 10A is a view cut along the line B1-B1 in FIG. 9B, and FIG. 10B is cut along the line C1-C1 in FIG. 9A. FIG. 10C is a cross-sectional view illustrating a part of FIG. 10B in an enlarged manner. 11A is an enlarged view of a part of FIG. 10A, and FIG. 11B is an enlarged view of a part of FIG. It is a figure which shows the fluid handling apparatus which concerns on a 1st prior art example. It is a figure which shows the fluid handling apparatus which concerns on a 2nd prior art example.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 ... Fluid handling apparatus, 7 ... 1st communication part, 10 ... 2nd communication part, 27 ... 1st main flow path, 28 ... 2nd main flow path, 30 ... 3rd main flow , 33... Sample introduction path (first fluid introduction path), 33 a... First main channel side connection part, 33 b... Main part, 41. Sub flow path 43... 3rd sub flow path 44... 4th sub flow path 45. 5th sub flow path 46... 6th sub flow path 47. Port ... 48 ... Second port, 49 ... Third port, 52 ... Sample (first fluid), 53 ... Polymer solution (second fluid), 54 ... Polymer solution (third fluid)

Claims (8)

A first main flow path through which a first fluid can move by capillary action;
A second main flow path in which the second fluid can move by capillary action;
A third main channel through which a third fluid can move by capillary action;
A first communication section that communicates the first main flow path, the second main flow path, and the external environment;
The first main flow path, the third main flow path, and a second communication part for communicating the external environment,
The first communication unit includes:
A first sub-flow channel formed so that the first fluid can move by capillary action, and communicates between the first main flow channel and the external environment;
A second sub-flow channel formed so that the second fluid is movable by capillary action and communicates between the second main flow channel and the external environment;
Having a cross-sectional area smaller than the cross-sectional area of the first main channel and the second main channel, the first fluid or the second fluid is movably formed by capillary action, and A third sub-flow channel connecting the first main flow channel and the second main flow channel,
The second communication unit is
A fourth sub-flow path formed so that the first fluid is movable by capillary action, and communicates the first main flow path and the external environment;
A fifth sub-flow channel formed so that the third fluid can move by capillary action and communicates between the third main flow channel and the external environment;
The first main flow path has a cross-sectional area smaller than the cross-sectional area of the first main flow path and the cross-sectional area of the third main flow path, and the first fluid or the third fluid is movably formed by capillary action, 1 main flow path and a sixth sub flow path connecting the third main flow path,
The first fluid that moves in the first main channel toward the first communication part and the second fluid that moves in the second main channel toward the first communication part are the Forming an interface at the first connecting portion;
The first fluid that moves in the first main channel toward the second connecting portion and the third fluid that moves in the third main channel toward the second connecting portion are the Forming an interface at the second connecting portion;
The first fluid is metered between the first communication portion and the second communication portion;
A fluid handling device. A first main flow path through which a first fluid can move by capillary action;
A second main flow path in which the second fluid can move by capillary action;
A third main channel through which a third fluid can move by capillary action;
A first communication section that communicates the first main flow path, the second main flow path, and the external environment;
The first main flow path, the third main flow path, and a second communication part for communicating the external environment,
The first communication unit includes:
A first sub-flow channel formed so that the first fluid can move by capillary action, and communicates between the first main flow channel and the external environment;
A second sub-flow channel formed so that the second fluid is movable by capillary action and communicates between the second main flow channel and the external environment;
Having a cross-sectional area smaller than the cross-sectional area of the first main flow path and the second main flow path, the first fluid and / or the second fluid is formed to be movable by capillary action, A third sub-flow path connecting the first main flow path, the second main flow path, and the external environment;
The second communication unit is
A fourth sub-flow path formed so that the first fluid is movable by capillary action, and communicates the first main flow path and the external environment;
A fifth sub-flow channel formed so that the third fluid can move by capillary action and communicates between the third main flow channel and the external environment;
The first main flow path has a cross-sectional area smaller than the cross-sectional area of the first main flow path and the cross-sectional area of the third main flow path, and the first fluid or the third fluid is movably formed by capillary action, 1 main flow path and a sixth sub flow path connecting the third main flow path,
The first fluid that moves in the first main channel toward the first communication part and the second fluid that moves in the second main channel toward the first communication part are the Forming an interface at the first connecting portion;
The first fluid that moves in the first main channel toward the second connecting portion and the third fluid that moves in the third main channel toward the second connecting portion are the Forming an interface at the second connecting portion;
The first fluid is metered between the first communication portion and the second communication portion;
A fluid handling device. A first main flow path through which a first fluid can move by capillary action;
A second main flow path in which the second fluid can move by capillary action;
A third main channel through which a third fluid can move by capillary action;
A first communication section that communicates the first main flow path, the second main flow path, and the external environment;
The first main flow path, the third main flow path, and a second communication part for communicating the external environment,
The first communication unit includes:
A first sub-flow channel formed so that the first fluid can move by capillary action, and communicates between the first main flow channel and the external environment;
A second sub-flow channel formed so that the second fluid is movable by capillary action and communicates between the second main flow channel and the external environment;
Having a cross-sectional area smaller than the cross-sectional area of the first main flow path and the second main flow path, the first fluid and / or the second fluid is formed to be movable by capillary action, A third sub-flow path connecting the first main flow path, the second main flow path, and the external environment;
The second communication unit is
A fourth sub-flow path formed so that the first fluid is movable by capillary action, and communicates the first main flow path and the external environment;
A fifth sub-flow channel formed so that the third fluid can move by capillary action and communicates between the third main flow channel and the external environment;
Having a cross-sectional area smaller than the cross-sectional area of the first main flow path and the third main flow path, the first fluid and / or the third fluid is formed to be movable by capillary action, A sixth sub-channel that communicates the first main channel, the third main channel, and the external environment;
The first fluid that moves in the first main channel toward the first communication part and the second fluid that moves in the second main channel toward the first communication part are the Forming an interface at the first connecting portion;
The first fluid that moves in the first main channel toward the second connecting portion and the third fluid that moves in the third main channel toward the second connecting portion are the Forming an interface at the second connecting portion;
The first fluid is metered between the first communication portion and the second communication portion;
A fluid handling device. A first port for introducing the first fluid into the first main channel is formed in the first main channel,
The second main channel has a second port for introducing the second fluid into the second main channel,
A third port for introducing the third fluid into the third main channel is formed in the third main channel.
The fluid handling device according to any one of claims 1 to 3, wherein   5. The fluid handling apparatus according to claim 4, wherein the first port is formed in the vicinity of the first connecting part or the second connecting part. The first port is connected to the first main flow path via a first fluid introduction path;
6. The fluid handling apparatus according to claim 4, wherein the first fluid is formed so as to be able to flow from the first fluid introduction path side to the first main channel side by capillary action. The first port is connected to the first main flow path via a first fluid introduction path;
The first main flow path side connection portion of the first fluid introduction path is formed in a cross-sectional area smaller than the main section of the first fluid introduction path and the flow path cross-sectional area of the first main flow path, 6. The fluid handling apparatus according to claim 4, wherein the first fluid is formed so as to be able to flow from the first fluid introduction path side to the first main flow path side by capillary action. A potential difference applying means for applying a potential difference is provided between the second main channel and the third main channel, and the charged substance contained in the first fluid in the first main channel is supplied to the second main channel. The fluid handling device according to any one of claims 1 to 7, wherein the fluid is electrophoresed to a channel or the third main channel.

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