JP4630161B2 - Whey protein food with intestinal regulation - Google Patents

Description

  The present invention relates to a whey protein food, and more particularly to a whey protein food having a better intestinal action.

  It is known that various microorganisms are established in the intestinal tracts of humans and animals and form a microflora. Among these microorganisms, there are microorganisms called bad bacteria that have a harmful effect on the host and good bacteria that have a beneficial action on the host. These microorganisms maintain a symbiotic or cohesive relationship. Examples of bad bacteria include those that produce harmful products such as ammonia, hydrogen sulfide, and amines, thereby overburdening liver function and related to carcinogenesis. Examples thereof include Clostridium such as Clostridium perfringens, enterococci, streptococci, and the like. Examples of good bacteria include bifidobacteria and lactobacilli.

  Improvement of intestinal microbiota, that is, the beneficial growth of the good bacteria is superior to that of bad bacteria, and as a beneficial effect brought to the host, improvement of digestive symptoms centering on diarrhea and constipation, Through immunostimulation, various factors that lead to a good intestinal environment necessary for the host to maintain a healthy life, such as cancer prevention, improvement of infection resistance, suppression of harmful microbial metabolites and harmful enzymes, etc. The effect of.

  Whey protein, on the other hand, is composed of amino acids similar to human milk and rich in nutritional value, contains all essential amino acids, has a very good absorption rate, and has a high content of branched-chain amino acids. As a dietary supplement, it is commercialized mainly for athletes and people with high exercise loads.

  Some whey proteins contain a large amount of antibodies derived from raw raw milk. Antibodies are also produced in the body in humans, but their production decreases with age, so the immune function of the elderly decreases. Antibody production is also reduced by the use of pharmaceuticals having stress and immunosuppressive effects.

  The antibodies in whey protein include antibodies that specifically bind to bad bacteria in the intestine. When the antibodies specifically bind to bad bacteria, they prevent harmful bacteria from entering the body through the intestinal wall. Because it is excreted together with the stool, it leads to improvement of the intestinal environment.

  Moreover, oligosaccharide and dietary fiber are mentioned as the food material which has an intestinal regulating action. Some oligosaccharides reach the large intestine without being degraded by digestive enzymes. This oligosaccharide that reaches the large intestine is considered to be useful for improving constipation and preparing the intestinal environment because it serves as a nutrient source for bifidobacteria. Dietary fiber also reaches the large intestine and becomes a nutrient source for bifidobacteria and other good bacteria, as well as promoting the adsorption and excretion of bad bacteria that interfere with the growth of good bacteria and harmful substances in the intestines.

  The present inventors have found that whey protein has an effect of improving stool odor, and that an improvement effect is increased when combined with an indigestible saccharide such as oligosaccharide, and previously proposed (Japanese Patent Application No. 2004-316331). 0015). However, foods with even stronger intestinal regulation are desirable and continued to be studied.

  An object of the present invention is to provide a whey protein food product having a stronger intestinal regulating action.

  As a result of intensive studies, the present inventors have found that a stronger intestinal action can be obtained by simultaneously ingesting three of whey protein, oligosaccharide and water-soluble dietary fiber, and have reached the present invention.

That is, the present invention is as follows.
1) Whey protein food characterized by containing whey protein, oligosaccharide and water-soluble dietary fiber, and 2) Whey protein food according to 1) above, wherein the shape is granular.

  According to the invention of claim 1, the whey protein itself has an intestinal regulating action, but by simultaneously ingesting the oligosaccharide and the water-soluble dietary fiber, the synergistic effect of the whey protein, the oligosaccharide and the dietary fiber is obtained. It is possible to provide a food that is exerted and has a stronger intestinal regulating action. According to the invention described in claim 2, in addition to this, it is possible to provide a whey protein food with good dispersibility when mixed with other foods and ingested.

  Milk used as a raw material for whey protein used in the present invention may be milk of any mammal such as cow, goat, sheep and horse. The milk may be milk collected from a mammal that has been vaccinated or milk collected from a mammal that has not been vaccinated.

  As these whey proteins derived from milk, ordinary commercially available milk-derived proteins, that is, whey protein concentrate (WPC), whey protein isolate (WPI), desalted whey powder and the like can be used. As for the separation method from raw milk, a microfiltration method, a cross-flow microfiltration method, an ion exchange method, or any other method can be used.

  Some whey proteins retain antibodies derived from raw raw milk. The antibody contained in such whey protein also contributes to the intestinal effect of the whey protein food of the present invention. Therefore, it is desirable that the whey protein used in the present invention contains 0.5% by mass or more of antibody.

  The oligosaccharides used in the present invention are disaccharides to decasaccharides that are difficult to digest with digestive enzymes in the human body, and any oligosaccharide that is generally used in foods can be used. Examples of these include, for example, fructooligosaccharides, dairy oligosaccharides (lactosucrose), lactulose, malto-oligosaccharides, isomalto-oligosaccharides, soybean oligosaccharides, galacto-oligosaccharides, xylooligosaccharides, raffinose, stachyose, palatinose oligosaccharides, nigero-oligosaccharides, gentioligosaccharides Examples thereof include sugar, trehalose, coupling sugar, pectin oligosaccharide, alginic acid oligosaccharide, guar gum oligosaccharide, fucose oligosaccharide, and cyclodextrin.

  In the present invention, dietary fiber refers to non-digestible carbohydrates that are not decomposed by digestive enzymes in the human body and bound to sugars that are more than enough, and are distinguished from indigestible carbohydrates. Dietary fiber is roughly classified into water-soluble dietary fiber and water-insoluble dietary fiber. In the present invention, water-soluble dietary fiber is used. Examples of water-soluble dietary fiber include pectin, guar gum, psyllium, galactomannan, xyloglucan, locust bean gum, glucomannan, polydextrose, soluble hemicellulose, sodium alginate, chondroitin sulfate, low molecular weight alginic acid, low molecular weight guar gum, fiberlon, etc. be able to. Moreover, you may use the food material containing these water-soluble dietary fibers, for example, agar, processed legumes, processed potatoes, etc.

  The whey protein food of the present invention preferably contains 5-1000 parts by mass of oligosaccharide and 5-1000 parts by mass of water-soluble dietary fiber with respect to 100 parts by mass of whey protein. If the content of the oligosaccharide or water-soluble dietary fiber is less than 5 parts by mass, the intestinal regulating effect is insufficient. On the other hand, if it exceeds 1000 parts by mass, the content of whey protein will be low, and the purpose of ingesting whey protein will be difficult to achieve.

  Further, when the whey protein food of the present invention is granulated, it can improve the dispersibility of the whey protein food when it is mixed with other foods, particularly foods with a high water content. And can be easily mixed with other foods. Whey protein has poor water dispersibility, and in the present invention, it contains oligosaccharides and dietary fiber, so that the dispersibility is somewhat improved over whey protein alone. However, when it is granulated and added to other foods such as milk and yogurt, the dispersibility is further improved, and it does not become “mamako” but can be easily mixed.

  The oligosaccharide is considered to be a binder at the time of granulation, but CMC (carboxymethylcellulose), a saccharide, or the like may be further added as a binder. The granulation can be performed by a usual method such as spray granulation method or extrusion granulation method. The size of the granule is not limited, but the average particle size is preferably about 0.02 to 2 mm.

  The whey protein food product can also contain ingredients that are usually added to foods, such as sweeteners, acidulants, fragrances, and thickening polysaccharides.

  The form of the whey protein food of the present invention is not limited. For example, in addition to the granules described above, tablets, candies, powders, capsules, pastes, and liquids may be used. If there is no heat processing step that deactivates the antibody after the addition of whey protein, it can take any processed food form.

  The present invention will be described in more detail with reference to examples below, but the present invention is not limited thereto.

[Example 1, Comparative Examples 1 and 2]
Three types of whey protein foods were prepared with the formulations shown in Table 1 below (numerical values are mass ratios). In Table 1, Proliant (registered trademark) 8000 is whey protein (WPC, manufactured by James Farrell & Co.), and Mayoligo (registered trademark) P is fructo-oligosaccharide (manufactured by Meiji Seika Co., Ltd.).

(Table 1)
Example 1 Comparative Example 1 Comparative Example 2
Proliant8000 90 90 90
Mayoligo P 5 10-
Pectin 5-10

(Measurement method of bifidobacteria and Clostridium perfringens)
Measurement of Bifidobacterium count: BL agar medium “Nissui” (Nissui Pharmaceutical Co., Ltd.) is sterilized, cooled to 50 ° C. and added to BS medium (30% sodium propionate, 6% lithium chloride, paromomycin sulfate 0) 0.1% neomycin sulfate 0.1%) and 5% equine defibrinated blood are added to a petri dish to form a flat plate. The diluted sample was applied with a large rod, and anaerobic culture was carried out at 37 ° C. for 20 hours to calculate.

Measurement of the number of Clostridium perfringens: Kanamycin-containing CW agar basal medium “Nissui” (Nissui Pharmaceutical Co., Ltd.) is sterilized and dispensed into a petri dish to form a flat plate. The diluted sample was applied with a large rod, and anaerobic culture was carried out at 37 ° C. for 20 hours to calculate.

  Three subjects were allowed to suspend the powders of Example 1, Comparative Example 1, and Comparative Example 2 in the order shown in Table 2 for 2 weeks (6 weeks in total), 10 g each day in 100 ml of water, I had you drink after every breakfast. Of each two weeks, feces were collected for the latter one week. In addition, feces were collected for one week before the start of the test. The number of bifidobacteria and Clostridium perfringens in these feces was measured by the above method, and the values in each test group were averaged. These measurement results are shown in the graph of FIG.

(Table 2)
Drinking order
Subject A Example 1 → Comparative Example 1 → Comparative Example 2
Subject B Comparative Example 1 → Comparative Example 2 → Example 2
Subject C Comparative Example 2 → Example 1 → Comparative Example 1

As shown in FIG. 1, Bifidobacteria, which are good bacteria, increased slightly when the whey protein foods of Comparative Examples 1 and 2 were ingested compared to before the start of the test, but greatly increased by ingesting those of Example 1. Increased. On the other hand, Clostridium perfringens, which is a bad bacteria, decreased when the foods of Comparative Examples 1 and 2 were ingested, and further decreased when the food of Example 1 was ingested, compared to before the start of the test.

  From these, it was shown that the intestinal environment was greatly improved by ingesting the whey protein food shown in Example 1 as compared with those of Comparative Examples 1 and 2.

[Example 2] (Examination of intestinal regulating action 1)
As subjects, 15 men and women (21 to 30 years old: 9 people, 31 to 40 years old: 3 people, 41 to 60 years old: 3 people) were divided into 3 groups (5 people in each group). The whey protein food powders of Examples 1 and 2 were suspended in 10 g of 100 ml of water per day and taken after breakfast. A two-week continuous drinking was conducted, and a questionnaire survey on defecation was conducted for the latter half of the week during continuous drinking. The same questionnaire survey is conducted for one week before continuous drinking. The survey items were (1) stool frequency, (2) stool volume, (3) stool color tone, (4) stool odor, and the results (mean value ± standard deviation of each group) are shown in Table 3.

(1) The number of defecations tended to increase when the whey protein food powders of Comparative Example 1 and Comparative Example 2 were drunk, but increased significantly when those of Example 1 were drunk. (2) There was no significant difference in the amount of defecation between the foods of Comparative Example 1, Comparative Example 2, and Example 1 due to drinking. (3) Although the tendency for a stool color tone to become favorable by drinking the foodstuff of the comparative example 1 and the comparative example 2 was seen, when the thing of Example 1 was drunk, the color tone was clearly improved. (4) Fecal odor was improved in Comparative Example 1 and Comparative Example 2, but was significantly improved in Example 1.

Summarizing (1) to (4), some whey protein foods of Comparative Example 1 and Comparative Example 2 have some intestinal regulating effects, but apparently by drinking the whey protein food of Example 1. Intestinal regulation was seen, and the superiority of taking the antibody, oligosaccharide and dietary fiber simultaneously was shown.

In Table 3, each numerical value means the following.
(3) Fecal color tone: 0: yellow, 1: light ocher, 2: ocher, 3: brown, 4: dark brown,
(4) Fecal odor: 0: Little concern, 1: Slight odor, 2: Normal, 3: Smell, 4: Smelly odor

(Table 3)
Before continuous drinking During continuous drinking
(1) Defecation frequency / week Comparative Example 1 5.7 ± 0.8 6.1 ± 0.6
Comparative Example 2 5.5 ± 0.8 5.9 ± 0.7
Example 1 5.8 ± 0.8 6.8 ± 0.6
(2) Defecation volume / week Comparative Example 1 28.7 ± 10.6 30.6 ± 10.7
Comparative Example 2 29.3 ± 10.5 29.8 ± 10.7
Example 1 29.6 ± 10.8 30.9 ± 10.6
(3) Fecal color tone Comparative Example 1 3.5 ± 0.8 2.8 ± 0.6
Comparative Example 2 3.7 ± 0.8 3.0 ± 0.7
Example 1 3.6 ± 0.8 1.5 ± 0.5
(4) Fecal odor Comparative Example 1 2.8 ± 0.6 1.5 ± 0.4
Comparative Example 2 2.6 ± 0.6 1.6 ± 0.5
Example 1 2.7 ± 0.6 0.4 ± 0.3

[Example 3] (Study 2 on intestinal regulating action)
A whey protein food product in which Mayoligo P of Example 1 and Comparative Example 1 was replaced with lactulose was prepared and tested in the same manner as in Example 2. As a result, the same results as in Example 2 were obtained, and antibodies and oligosaccharides were obtained. And the superiority of taking the three of dietary fiber at the same time was shown.

[Example 4] (Study 3 on intestinal regulation)
A whey protein food product in which Mayoligo P of Example 1 and Comparative Example 1 was replaced with trehalose was prepared and tested in the same manner as in Example 2. As a result, the same results as in Example 2 were obtained. And the superiority of taking the three of dietary fiber at the same time was shown.

[Example 5] (Study 4 on intestinal regulating action)
A whey protein food product in which the pectin of Example 1 and Comparative Example 2 was replaced with sodium alginate was prepared and tested in the same manner as in Example 2. As a result, the same results as in Example 2 were obtained. And the superiority of taking the three of dietary fiber at the same time was shown.

[Example 6] (Study 5 on intestinal function)
A whey protein food product in which the pectin of Example 1 and Comparative Example 2 was replaced with glucomannan was prepared and tested in the same manner as in Example 2. As a result, the same results as in Example 2 were obtained. And the superiority of taking the three of dietary fiber at the same time was shown.

2 is a graph showing the number of bifidobacteria and the number of Clostridium perfringens in feces measured in Example 1 and Comparative Examples 1 and 2.

Claims (2)

Whey protein containing 0.5% by mass or more of antibody, 5-1000 parts by mass of indigestible oligosaccharide with respect to 100 parts by mass of whey protein, and 5-1000 parts by mass with respect to 100 parts by mass of whey protein. Whey protein food , which is composed of water-soluble dietary fiber and has an intestinal regulating action .   The whey protein food product according to claim 1, wherein the whey protein food product is granular.

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