JP4334956B2 - Cell activators, whitening agents, and antioxidants - Google Patents

Description

The present invention relates to a cell activator, a whitening agent, and an antioxidant. More specifically, cell activator to Hanayasuri genus (Ophioglossum) active ingredient a plant extract Hanayasuri family (Ophioglossaceae), whitening agents, and to antioxidants.

  A decrease in skin cell function due to aging causes a decrease or degeneration of the dermal matrix such as collagen and elastin, and is an important factor of aging symptoms such as wrinkles and a decrease in skin elasticity. In addition, oxidative damage caused by extraneous stress such as ultraviolet rays also causes aging symptoms such as wrinkles, spots, and a decrease in skin elasticity. So far, various cell activators, whitening agents, and antioxidants have been searched and formulated for the purpose of preventing / improving aging symptoms due to cell functional deterioration and oxidative damage. As a cell activator, the essence of Ponkan (see Patent Document 1), the genus Penicillium, the wedge and their extracts (see Patent Document 2), and the chlorella extract using an organic solvent (see Patent Document 3) are known. As the whitening agent, cranberry fruit press filter and / or extract mainly containing anthocyanin polymer (see Patent Document 4), white crane reishi water and / or organic solvent extract (see Patent Document 5) are known. It has been. Further, as an antioxidant, an Asteraceae Heteroteca genus plant extract (see Patent Document 6) and a Sargoceae family Sarogase genus plant extract (see Patent Document 7) are known.

Incidentally, cell activator to Hanayasuri genus (Ophioglossum) active ingredient a plant extract Hanayasuri family (Ophioglossaceae), the prior art was observed regarding whitening agents and antioxidants.

JP 2001-131045 A JP 2000-178198 A JP 11-335293 A JP 2002-3361 A JP 2003-89630 A JP-A-11-180886 Japanese Patent Laid-Open No. 10-182413

  Conventionally used cell activators, whitening agents, and antioxidants may be insufficient as essential effects, and development of better active ingredients has been expected. The present invention has been made in view of such conventional problems, and provides a cell activator, a whitening agent, and an antioxidant that are naturally derived, highly safe, and exhibit excellent effects. Objective.

  In order to solve the above-mentioned problems, the present inventors have studied cell activation, whitening and antioxidant effects of various naturally-derived substances. As a result, an excellent cell activation action, whitening action, and antioxidant action were found in the extract of the plant of the genus Papaver, and further studies were made to complete the present invention. That is, the present invention provides a cell activator, a whitening agent, and an antioxidant, each of which contains an extract of one or more plants of the genus Hyacinth as an active ingredient.

  ADVANTAGE OF THE INVENTION According to this invention, the cell activator, whitening agent, and antioxidant which have the outstanding effect can be provided. In addition, by adding such cell activators, whitening agents, and antioxidants, topical skin preparations and foods that exhibit excellent effects in preventing and improving skin symptoms such as wrinkles, tarmi, skin firmness, spots, and rashes. The composition can be provided.

The plants used in the present invention is not particularly limited as long as Hanayasuri genus (Ophioglossum) plant Hanayasuri family (Ophioglossaceae). There are about 40 species of the genus Hyanasalis , such as Ophioglossum vulgateum L., Coblanc ( Ophioglossum pendulum ), and Ophioglossum kawamurae . The raw material used in the present invention is not particularly limited as long as it is a plant belonging to the genus Hana file, but for the reason that it is relatively easy to obtain, it is preferably used.

  An extract is generally used when using these plants. For extraction, any part of the roots, leaves, flowers, stems, stem skins, etc. of the genus Hyacinth can be used, but whole plants are preferably used for convenient use. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, the mixture may be homogenized in stirring or an extraction solvent. Although extraction temperature is not specifically limited, It is preferable to set it as the temperature below about the boiling point of an extraction solvent from about 5 degreeC. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but is preferably about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Extracts from the above-mentioned genus Hyacinth can be used as they are, but they can be decolorized and deodorized as long as the concentrated and dried solids are dissolved again in water or a polar solvent, or the physiological effects thereof are not impaired. It may be used after a purification treatment such as desalting or a fractionation treatment by column chromatography or the like. The above-mentioned extract of the genus Hyacinth, the processed product and the fraction thereof can be freeze-dried after each treatment and fractionation and dissolved in a solvent before use.

  The cell activator, the whitening agent, and the antioxidant in the present invention contain the above-mentioned plant extract of the genus Genus as an active ingredient. The obtained cell activator exerts an excellent activation action on various cells, but particularly exhibits an excellent effect on dermal fibroblasts and epidermal cells. Further, the obtained whitening agent is effective in improving pigmentation symptoms such as spots and freckles, and in particular, is excellent in suppressing melanin production in melanoma cells. Furthermore, the obtained antioxidant exhibits an excellent antioxidant effect, but particularly exhibits an excellent effect on free radical scavenging action.

  Moreover, the composition which has a cell activation effect | action, a whitening effect | action, and an antioxidant effect | action by mix | blending a cell activator, a whitening agent, and an antioxidant which use the extract of a genus genus plant into an active ingredient in various compositions. Can be obtained. The obtained composition is particularly preferably used as a skin external preparation or food from the viewpoint of effectiveness such as cell activation, whitening and antioxidant. These skin external preparations and foods can be used as cell-applied skin external preparations, whitening skin external preparations, antioxidant skin external preparations, cell-applied foods, whitening foods, and antioxidant foods.

  The amount of the extract of the genus plant in these compositions can be adjusted according to the type of composition and the intended purpose of use, but in the case of external preparations for skin and foods, from the point of effect and stability. The amount is preferably 0.0001 to 10.0% by weight, more preferably 0.001 to 5.0% by weight, based on the total amount.

  The dosage form of the external preparation for skin containing the genus plant extract is arbitrary, and can be provided, for example, as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  In addition, for the topical skin preparation containing the extract of the plant of the genus Papaver, in addition to the extract of the plant of the genus P. Formulated oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, cleaning agents, UV absorbers, thickeners, chemicals, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. can do. Moreover, in the range which does not impair the effect of this invention, combined use with another cell activator, whitening agent, and antioxidant is also possible.

  In addition, foods that contain the extract of the genus Hyacinth are: oral compositions such as gums and candies, marine products such as kamaboko and chikuwa, livestock products such as sausages and ham, western confectionery, Japanese confectionery, raw noodles, boiled Noodles such as noodles, seasonings such as sauces, soy sauce and sauce, pickles, prepared dishes, soft drinks, and other general food and drink dosage forms can be obtained. At that time, within the range not impairing the effect of the present invention, various components commonly used in foods, for example, sugar, condensed milk, flour, shortening, salt, glucose, chicken egg, butter, margarine, starch syrup, calcium, iron, It can be used in combination with seasonings, spices and the like.

  Further, the features of the present invention will be described in detail by way of examples. First, it shows about the manufacture example of the flower genus plant extract of this invention.

[Production Example 1]
10 kg of a 50 wt% aqueous ethanol solution was added to 1 kg of a dry pulverized whole plant of the genus Hyacinth and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, a flower extract was obtained.

[Production Example 2]
Nine liters of water was added to 1 kg of a dry pulverized whole plant of the genus Hyacinth and extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, a flower extract was obtained.

[Production Example 3]
Nine liters of methanol was added to 1 kg of a dry pulverized whole plant of the genus Hyacinth and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, a flower extract was obtained.

[Production Example 4]
The whole plant of the genus Hyacinth was introduced into a supercritical extraction apparatus and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected to obtain a genus plant extract.

  Next, it shows about the activation effect | action of the dermal fibroblast of the genus plant of the genus Hyanas. As the sample, the extract of Hirahana narisari extracted from Hilohanahanasori using Production Example 1 was used.

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Next, the medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample as 100. In the table, * and ** indicate a significance probability of less than 5% (P <0.05) with respect to the significance probability P value in the t-test, and a significance probability of less than 1% (P <0.01). It is represented by **.

  As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the extract of Hirohanahanasari. In particular, when adding 0.031 to 0.25 mg / mL of Hirohanahanasari extract, a significant dermal fibroblast activation effect was observed with a risk rate of less than 1% compared to the blank. From this, it was clarified that Hiroha Hana Yasuri extract has an excellent dermal fibroblast activation effect.

  Next, it shows about the activation effect | action of the epidermis cell of a flower-genus plant extract. As the sample, the extract of Hirahana narisari extracted from Hilohanahanasori using Production Example 1 was used.

The evaluation was performed according to the following procedure. Normal human epidermal cells were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. As a seeding medium, commercially available Humdia-KG2 manufactured by Kurabo Industries Co., Ltd. was used. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 24 hours. Next, the medium containing 100 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan generated by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 2 as relative values when the cell activation effect in the blank with no sample is taken as 100. In addition, * and ** in the table represent ** with a significant difference observed at a risk rate (P <0.01) with a significance probability of less than 1% with respect to the significance probability P value in the t-test. Is.

  As shown in Table 2, when the broad-leaf extract was added, a significant epidermal cell activation effect was observed compared to the blank. In particular, when 0.063 mg / mL of the broad-leaf extract was added, a significant epidermal cell activation effect was observed with a risk rate of less than 1% compared to the blank. From this, it was clarified that the extract of Hiroha Hana Yasuri has an excellent epidermal cell activation effect.

  Next, the evaluation of the melanin production inhibitory effect of the genus plant extract is shown. As the sample, the extract of Hirahana narisari extracted from Hilohanahanasori using Production Example 1 was used.

  The evaluation was performed according to the following procedure. B16 mouse melanoma F0 strain (B16F0) cells were seeded in a 35 mm dish at 2000 cells per dish. After culturing for 24 hours, the culture medium was replaced with Dulbecco's modified Eagle medium (DMEM) supplemented with 5 wt% fetal calf serum (FCS) to which a sample of an arbitrary concentration was added. After culturing for 7 days, cells were harvested using 0.25% trypsin, transferred to a 1.5 mL microtube and centrifuged to obtain a cell precipitate. Finally, the color of the precipitate was visually determined based on the determination table shown in Table 3. Furthermore, instead of the medium to which the sample was added, a Dulbecco's modified Eagle medium (DMEM) to which 5 wt% fetal calf serum (FCS) was added was used as a blank, and visual determination was similarly performed. Further, as shown in Table 3, the visual judgment was performed by a five-step evaluation. Table 4 shows the determination results.

  As is clear from Table 4, a significant melanin production inhibitory effect was observed when using a medium supplemented with 0.13 to 1.0 mg / mL of the broad-leaf extract. In particular, no blackening was observed when 1.0 mg / mL of the broad-leaf extract was added. From this, it was clarified that the extract of Hirahana naseri has an excellent melanin production inhibitory action and an excellent whitening effect.

  Next, it demonstrates about the antioxidant effect of a genus plant extract. As the sample, the extract of Hirahana narisari extracted from Hilohanahanasori using Production Example 1 was used.

The evaluation was performed according to the following procedure. 100 μL of Hiroha Hana Yasuri extract solution diluted to an arbitrary concentration with a 50 wt% aqueous ethanol solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 5.
Formula (1) {1- (B) / (A)} × 100 (%)

  As is clear from Table 5, it was found that the extract of Hirahana natsuri has an excellent antioxidant effect.

  Subsequently, an example of the formulation of a topical skin preparation and food containing the extract of the plant of the genus Papaver according to the present invention will be shown.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 50.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Hiroha Hanaya File Extract [Production Example 1] 8.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Hiroha Hana File Extract [Production Example 3] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Hiroha Hanaya File Extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) Hiroha Hana File Extract [Production Example 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Hirohanahanasari Extract [Production Example 4] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Hiroha Hana File Extract [Production Example 4] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Hiroha Hana File Extract [Production Example 3] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Hiroha Hanaya File Extract [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Hiroha Hanaya File Extract [Production Example 4] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Hiroha Hana File Extract [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melt | dissolved at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 10.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Hiroha Hana File Extract [Production Example 2] 12.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Hiroha Hana File Extract [Production Example 1] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) Hiroha Hana File Extract [Production Example 1] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification is completed, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hair artic (1) Ethanol 50.0 (% by weight)
(2) Purified water 48.9
(3) Hiroha Hana File Extract [Production Example 3] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

[Prescription Example 15] Beverage (1) Hiroha Hana Yasuri Extract [Production Example 1] 5.0 (% by weight)
(2) Erythritol 1.0
(3) Citric acid 0.1
(4) Stevia 0.01
(5) Purified water 93.89
Production method: (1) to (5) are mixed uniformly.

[Prescription Example 16] Candy (1) Sucrose 50.0 (parts by weight)
(2) Minamata 44.9
(3) Hiroha Hana File Extract [Production Example 1] 5.0
(4) Fragrance 0.1
Production method: (1) to (2) are heated, mixed and homogenized, cooled, added with components (3) to (4) at 70 ° C., mixed and homogenized, and then molded.

  Next, a use test was conducted using a prescription blended with an extract of a genus plant, and the improvement effect was evaluated for wrinkles, tarmi, skin firmness, stains, and kusumi. At that time, each of the genus plant extracts listed in Table 6 was added to the emulsion formulation shown in Formulation Example 1, and the use test was conducted as Examples 1-4. In addition, as a comparative example 1, a use test was performed at the same time, substituting the extract of the plant of the genus Prunus with purified water.

  For each sample, each group of 20 male and female panelists in their 40s to 60s who have noticeable symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumi are used as a group for 2 months. Changes in skin condition were observed and evaluated. As indicators of skin symptoms, wrinkles, tarmi, skin firmness, spots, and kusumi were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. Showed.

  From Table 7, for wrinkles, tarmi, skin firmness, stains, and kusumi, in the comparative example use group that did not contain the extract of the genus plant, no improvement was observed in more than 60% of the panelers, but the extract of the genus plant was In the example use group in which the product was blended, 60% or more of panelists clearly improved.

  As described above, in the examples of the present invention, the effects of improving wrinkles, tarmi, skin firmness, spots, and stains were superior to those of the conventional comparative example.

Claims (6)

A cell activator comprising, as an active ingredient, an extract of one or more plants of the genus Ophioglossum belonging to the family Ophioglossaceae . Cell activator according to claim 1 Hanayasuri genus (Ophioglossum) plant is Hirohahanayasuri (Ophioglossum vulgatum L.) of Hanayasuri family (Ophioglossaceae). Hanayasuri genus (Ophioglossum) whitening to one or more of the active ingredient extracts of plants of the plant of Hanayasuri family (Ophioglossaceae). Whitening agent of claim 3 Hanayasuri genus (Ophioglossum) plant Hanayasuri family (Ophioglossaceae) is Hirohahanayasuri (Ophioglossum vulgatum L.). An antioxidant containing as an active ingredient an extract of one or more plants of the genus Ophioglossum belonging to the family Ophioglossaceae . Antioxidants according to claim 5 Hanayasuri genus (Ophioglossum) plant is Hirohahanayasuri (Ophioglossum vulgatum L.) of Hanayasuri family (Ophioglossaceae).