JP3885204B2 - External agent for promoting cell activity - Google Patents

Description

【００２６】
表１は、処方例１の薬理試験による結果を示すものである。このときの有効性とは、本発明外用剤の使用により、変化なしを０ポイント、治癒に向う変化があったものを１ポイント、効果が確認されたものを２ポイントとした各人の合計点を出し、次の式で算出したものである。尚、今回の試験は、特に花粉症の治癒効果を確認するために、疾患者１００人を対象として有効率を算出した。
【００２７】
【数１】

【００２８】
この結果、１００人の花粉症を対象にしても98％の有効率が得られた。前回（平成2年）の試験において、花粉症で100％を示していた有効率は、その後、十数年を経過した今日、発症率の爆発的増加に反比例するように、有効率が急激に下降していたが、本発明によって98％の有効率を維持できるようになっていることは特筆すべき効果であるといえる。また、600症例中、ただの一例も副作用が認められず、しかも、男性型脱毛症やニキビ、水虫、脂漏性皮膚炎など、治癒改善が困難な疾患に対しても極めて有効である。
【００２９】
【発明の効果】
本発明によると、全体の重量比で、デキストラン１８％、ブドウ糖１０％、サイクロデキストリン２％、トレハロース３％、プルラン７％、レンチナン７％、ラミナラン２％、クレスチン１％、ペクチン３％、キシログルカン１％、塩化ナトリウム０．９％、塩化カルシウム０．３％、塩化カリウム０．３％、精製水４４．５％の各成分を、水相成分に添加したことにより、口腔や鼻粘膜等の細胞組織の免疫力や治癒力を高めることが可能になり、花粉症などの個人的要因が作用する疾患でも有効な治癒効果が得られる。
【００３０】
また、細胞組織の免疫力や治癒力を高めながら皮膚細胞間質液と同様な環境を作り出すことができるので、ミトコンドリアの膜の障害によって生じる、細胞内呼吸の阻害や、細胞の活動源たるATP（アデノシン３リン酸）の産生の低下を防止することができ、電解質バランス、浸透圧バランスを保ち、皮膚細胞や粘膜細胞の表層からも障害細胞の正常化を促進することが可能になり、男性型脱毛症やニキビ、水虫、脂漏性皮膚炎など、治癒改善が困難な疾患に対しても極めて有効であり、本発明の意義は極めて大きく、人類に大きく貢献するものである。[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an agent for promoting cell activity, mainly having an improvement effect on various diseases such as hay fever and stomatitis, and activating cells by applying electrolyte balance and osmotic pressure balance to the cells. The present invention relates to an agent for promoting cell activity that improves various diseases caused by a decrease in skin cell function.
[0002]
[Prior art]
Conventionally, inorganic salts such as sodium chloride, natural sugars such as glucose, or plasma have been widely used as a formulation for cosmetics and pharmaceutical external preparations. For example, a cream relating to a soothing skin containing sodium chloride or the like is described in USP3574854, while an application for sanitizing skin containing mineral salts is disclosed in West German Patent Application No. 3327840. It can be seen. Further, an application relating to a sugar component that smoothes skin mixed with glucose or the like is described in USP3859436, and an application relating to a dextran aqueous solution for shaving is described in USP3777597.
[0003]
However, in these conventional external preparations, even if the purpose of skin sterilization and protection of the skin is achieved, skin diseases caused by the circulatory and functional deterioration of the skin capillaries, i.e., pigmentation, athlete's foot, insect, worm, hair loss, hair loss It has been difficult to ameliorate various diseases caused by reduced skin cell division function such as symptom, dandruff and itching.
[0004]
Therefore, the present inventor has created an environment similar to the skin cell interstitial fluid on the skin surface to activate the skin cells and improve various diseases caused by the decreased cell functions of the skin cells, and to maintain the electrolyte balance and osmotic pressure balance. By applying it, the inventors have invented aqueous skin and hair cosmetics that can promote normalization of damaged cells from the surface of the skin (Japanese Patent No. 1597430).
[0005]
In the case of this aqueous skin and hair cosmetic, in the case of spots and red face, the subcutaneous cells are stimulated and divided, leading to healing. The effect of stimulating hair and promoting hair growth and deodorizing action was observed. In other words, the aqueous skin and hair cosmetics have obtained efficacy results such as a cure rate for alopecia of 83%, a cure rate for pigmentation of 95%, and a cure rate of red face of 53%.
[0006]
However, these effects are recognized mainly for skin and hair, and the present inventors activate mucosal cells without side effects such as activation of skin cells and the like. If possible, we focused on the possibility that the effect could also be applied to diseases such as stomatitis due to oral mucosal disease and hay fever due to damage to the nasal mucosa.
[0007]
Therefore, the present inventor further has no fear of side effects even when used as an external preparation for oral cavity or nasal mucosa, and can be applied to various diseases in a wider range of cells such as stomatitis and hay fever. Has invented an external preparation for promoting cell activity (Japanese Patent No. 2681527). This external preparation for promoting cell activity is obtained by adding polysaccharides such as dextran, glucose, mutan, lentinan and the like. With this cell activity promoting external preparation, excellent results have been obtained, such as a cure rate for stomatitis of 100% and a cure rate for hay fever of 100%.
[0008]
[Problems to be solved by the invention]
However, the cure rate for hay fever shown in the above-mentioned external preparation for promoting cell activity was shown at the time when the number of subjects was 40, and as the number of patients with hay fever increased rapidly thereafter, pollen The cure rate of the disease tended to decrease gradually. In other words, the effectiveness rate, which showed 100% in hay fever in the previous efficacy test (Heisei 2), is an effective rate so that it is inversely proportional to the explosive increase in the incidence rate after 10 years. It has been found that is falling rapidly. In the first place, the cause of the development of hay fever has not yet been clarified, but it is thought that personal factors have some influence on the cellular level of the oral cavity and nasal mucosa to induce the onset. Therefore, by improving the immunity and healing power of the oral cavity, nasal mucosa, etc. and keeping the cellular tissue in a healthy state, it becomes possible to prevent adverse effects due to individual factors and suppress the onset of rapidly increasing hay fever. At the same time, it is considered that a healing effect is also produced.
[0009]
Therefore, the present invention further improves the immunity and healing power of cell tissues such as the oral cavity and the nasal mucosa by adding further improvements to the cell activity promoting external preparation previously invented, and there is no risk of side effects. It is an object of the present invention to provide an external preparation for promoting cell activity that can be applied not only to stomatitis but also to various diseases in a wide range of cells such as skin and scalp.
[0010]
[Means for Solving the Problems]
In order to achieve the above-mentioned object, the first means of the present invention is as follows: in an aqueous solution in which a polysaccharide is dissolved , dextran 18%, glucose 10%, cyclodextrin 2%, trehalose 3%, pullulan 7 in the total weight ratio. %, Lentinan 7%, laminaran 2%, krestin 1%, pectin 3%, xyloglucan 1%, sodium chloride 0.9%, calcium chloride 0.3%, potassium chloride 0.3%, purified water 44.5% This is because all the components were added.
[0011]
Causes of various symptoms such as inflammation, pigmentation, and alopecia that are lesions in human skin are related to various factors such as ultraviolet rays and chemical substances, and their changes at the cellular level are limited. The root of this is a disorder of the mitochondrial membrane and cell membrane, one of the organelles. In other words, damage to the mitochondrial membrane results in inhibition of intracellular respiration, thereby reducing the production of ATP (adenosine triphosphate), which is the source of cellular activity. Such a deficiency in ATP reduces the function of active transport of the cell membrane, leading to a decrease in the ability to transport substances between cells and interstitial fluid. This causes a vicious circle in which intracellular deprivation substances such as glucose are deficient, leading to a decrease in the acidity of ATP.
[0012]
There are a variety of causes that cause changes at the cellular level as described above. In particular, in cells such as the oral cavity and nasal mucosa, the cells in the diseased part are removed by raising the cell interstitial fluid by osmotic pressure action. Can be activated. Furthermore, it is considered that by adding xyloglucan, lamiranan and krestin, the immunity and healing power of cell tissues such as the oral cavity and nasal mucosa can be enhanced.
[0013]
DETAILED DESCRIPTION OF THE INVENTION
The basic formulation of the present invention is to add each component of xyloglucan, lamiranan, and krestin to an aqueous solution in which a polysaccharide is dissolved.
[0014]
Xyloglucan is a constituent sugar chain that exists universally on the wall (primary wall) of plant cells that are elongated and enlarged. Plant species specificity arises from the binding of galactose or fucosyl-galactose to xylose residues. Lectins can bind to these galactose and fucose residues, but the functions of these branched sugar chains are not known. Plant cell growth is caused by a water absorption phenomenon derived from the osmotic pressure of the cell, and water absorption is caused by a decrease in wall pressure due to loosening of the cell wall. This loosening of the cell wall has not yet been elucidated, but cell elongation is always accompanied by degradation and solubilization of xyloglucan, and is attracting attention as one of the polysaccharides that control the physiological activity of cells.
[0015]
Ramilanan is one of the carbohydrates and is classified as a β-glucan lamiranan. It is contained in seaweeds such as shiitake mushrooms and kelp and has the effect of enhancing immunity. It is known that it is difficult to absorb by itself and should be taken with protein.
[0016]
Krestin is used as an immunotherapeutic drug that enhances the expression of HLA class I antigens in human cancer cells, and is extracted from chopped mycelium. This krestin has been shown to have an immunotherapeutic effect on neoplastic diseases such as gastric cancer, colorectal cancer, and small cell lung cancer.
[0017]
As a result of researching ingredients suitable for external preparations from among many polysaccharides, the inventor has combined these xyloglucans, lamiranans, and krestins to immunize and heal cellular tissues such as the oral cavity and nasal mucosa. This is a preparation for external use that increases strength.
[0018]
In an aqueous solution in which these polysaccharides are dissolved, each component of pectin, dextran, glucose, lentinan, and trehalose is previously contained in a total weight ratio.
[0019]
Pectin functions to connect plant cells by binding to other components such as cellulose as a component of plant cell walls. It is a component having a gelling action and is suitable as an additive for the external preparation of the present invention.
[0020]
Moreover, sodium chloride, calcium chloride, and potassium chloride are added to the aqueous solution in which the polysaccharide is dissolved. The amount of sodium chloride is 0.9% in terms of the total weight ratio so that the osmotic pressure balance and the electrolyte balance on the skin, that is, the skin cells can be activated, and similarly 0.3% in calcium chloride and potassium chloride. % Of each component is dissolved in the aqueous phase component.
[0021]
【Example】
Next, the preparation for external use of the present invention will be specifically described with reference to formulation examples and pharmacological test examples.
[0022]
-Prescription example-
Dextran 18
Glucose 10
Cyclodextrin 2
Trehalose 3
Pullulan 7
Lentinan 7
Lamina Run 2
Krestin 1
Pectin 3
Xyloglucan 1
Sodium chloride 0.9
Calcium chloride 0.3
Potassium chloride 0.3
Purified water 44.5
Total weight ratio (%) 100
[0023]
The water phase component (purified water) is a non-oil produced only with pure water, and does not contain any water-soluble petroleum-based surfactants used at the time of producing ordinary external preparations such as cosmetics. And when mixing each of these components and an aqueous phase component, in order to complete melt | dissolution of each component, it implements at the temperature of 50-100 degreeC.
[0024]
Next, Table 1 shows the effects of Formulation Example 1.
[0025]
[Table 1]

[0026]
Table 1 shows the results of the pharmacological test of Formulation Example 1. The effectiveness at this time is the total score for each person, with 0 points indicating no change, 1 point indicating a change in healing, and 2 points indicating a confirmed effect due to the use of the external preparation of the present invention. And calculated by the following formula. In addition, in this test, in order to confirm the curative effect of hay fever in particular, the effective rate was calculated for 100 patients with disease.
[0027]
[Expression 1]

[0028]
As a result, an effective rate of 98% was obtained even for 100 people with hay fever. The effectiveness rate, which showed 100% in hay fever in the previous study (Heisei 2), has increased sharply so that it is inversely proportional to the explosive increase in the incidence rate after 10 years. Although it was decreasing, it can be said that it is a remarkable effect that the effective rate of 98% can be maintained by the present invention. In addition, only one of the 600 cases has no side effects, and is extremely effective for diseases that are difficult to improve, such as androgenetic alopecia, acne, athlete's foot, and seborrheic dermatitis.
[0029]
【The invention's effect】
According to the present invention, dextran 18%, glucose 10%, cyclodextrin 2%, trehalose 3%, pullulan 7%, lentinan 7%, laminaran 2%, krestin 1%, pectin 3%, xyloglucan By adding each component of 1%, sodium chloride 0.9%, calcium chloride 0.3%, potassium chloride 0.3%, purified water 44.5% to the aqueous phase component, It becomes possible to enhance the immunity and healing power of the cell tissue, and an effective healing effect can be obtained even in diseases in which individual factors such as pollinosis act.
[0030]
In addition, it can create an environment similar to that of skin cell interstitial fluid while enhancing the immunity and healing power of cellular tissues, so that inhibition of intracellular respiration caused by mitochondrial membrane damage and ATP as the source of cellular activity (Adenosine triphosphate) production can be prevented, electrolyte balance and osmotic pressure balance can be maintained, and normalization of damaged cells can be promoted from the surface layer of skin cells and mucosal cells. The present invention is extremely effective even for diseases that are difficult to improve, such as type alopecia, acne, athlete's foot, and seborrheic dermatitis, and the significance of the present invention is extremely great and greatly contributes to mankind.

Claims (1)

In an aqueous solution in which the polysaccharide is dissolved, dextran 18%, glucose 10%, cyclodextrin 2%, trehalose 3%, pullulan 7%, lentinan 7%, laminaran 2%, krestin 1%, pectin 3 %, Xyloglucan 1%, sodium chloride 0.9%, calcium chloride 0.3%, potassium chloride 0.3%, purified water 44.5% Agent.