JP3532244B2 - Mucopolysaccharide fragmentation inhibitor, active oxygen scavenger and cosmetic - Google Patents

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a mucopolysaccharide fragmentation inhibitor extracted from plant petals, an active oxygen scavenger, and cosmetics containing these as active ingredients. In particular,
The cosmetics according to the present invention are mainly intended to provide anti-aging cosmetics for skin and the like, but the field of application of the present invention is not limited to the cosmetics field of the cosmetics, and pharmaceuticals, foods, etc. It can be widely applied to various technical fields.

[0002]

Needless to say, oxygen is indispensable for aerobic organisms such as mammals such as humans. However, in recent years, it is said that oils and fats become lipid peroxides and damage cells by active oxygen generated in the living body. In addition, active oxygen also affects proteins, sugars, nucleic acids, etc., causing cross-linking and fragmentation of collagen,
It is believed to fragment against hyaluronic acid. In addition, it has been shown to cause damage to various tissues, and these damages cause arterial effects and inflammation,
Furthermore, it has been shown to be associated with aging and carcinogenesis.

[0003] The skin is not only in contact with the outside air but is constantly exposed to ultraviolet rays, and is said to be the tissue most susceptible to oxidative stress.

Mucopolysaccharides represented by hyaluronic acid are
It is said to be fragmented by active oxygen and ultraviolet rays, leading to skin aging. Therefore, suppressing the fragmentation of mucopolysaccharides present in the dermis of the skin is important for preventing skin aging. Furthermore, the sebum secreted on the skin is oxidized by ultraviolet rays to become lipid peroxides, and this lipid peroxide causes irritation to the skin, and
It is said to be involved in aging by attacking cells to cause damage or causing various other adverse effects. Therefore, it is considered that suppressing the production of lipid peroxide is effective not only for preventing the deterioration of the skin (skin) condition but also for preventing the aging of the living body itself.

Active oxygen includes superoxide anion radical (O ₂ ⁻ ), hydroxy radical (.OH), singlet oxygen ( ¹ O ₂ ), hydrogen peroxide (H ₂ O ₂ ), and the like.
Also known are peroxy radicals and alkoxy radicals generated by the peroxidation of lipids.

However, the reactive oxygen species generated in the living body are erased by the antioxidants provided in the living body.
Its anti Oxidizing substances superoxide as the enzyme (O ₂ ^-) oxidized glutathione superoxide dismutase to be erased (SOD) and hydrogen peroxide catalase and reduced glutathione for erasing _{(H 2 O 2) (GSH} ) and Glutathione peroxidase, which reduces peroxide by changing to (GSSG), is available. Other low molecular weight substances include proteins such as transferrin and ceruloplasmin, vitamin E, coenzyme Q, vitamin C,
Examples include glutathione, uric acid, bilirubin, and metallothionein.

[0007] The active oxygen produced by formulating these is eliminated, and it is intended to be useful for prevention of inflammation, aging, cancer and the like caused by them. However, regarding the enzyme, there is a problem in that it is not decomposed by digestive enzyme orally when it is orally administered, and it does not reach the site of action due to difficulty in percutaneous absorption, and there is a problem in stability. With respect to low molecular weight substances as well, there is a problem with stability and it has not yet been put to practical use.

Therefore, vitamin C and vitamin E have been widely used in recent years, but problems such as insufficient stability and active oxygen scavenging action remain.

In active oxygen, superoxide anion radicals are eliminated by SOD to produce hydrogen peroxide and water. It is said that the administration of SOD alone is not appropriate because the hydrogen peroxide generated here also gives a strong damage to the living body.

[0010]

DISCLOSURE OF THE INVENTION The present invention is directed to searching mucopolysaccharides, which are the main constituents of the skin, in order to search for a substance having an action of suppressing skin aging from extracts of various plant petals. As a result of research aiming at searching for those having an inhibitory effect on fragmentation by oxygen and searching for those having an active oxygen scavenging effect that causes various adverse effects such as aging, mucopolysaccharide fragmentation inhibitory effect It was completed based on the discovery of a new one having the ability to scavenging active oxygen and a new one having the ability to eliminate active oxygen.

Therefore, the present invention utilizes a mucopolysaccharide fragmentation inhibitor and an active oxygen scavenger which are stable and safe without side effects obtained from extracts of petals of natural products (plants). The purpose of the present invention is to provide cosmetics (antiaging cosmetics), and the active ingredients of the mucopolysaccharide fragmentation inhibitor and active oxygen scavenger according to the present invention are not only used in the field of cosmetics but also in pharmaceuticals and foods. It also opens up a wide range of applications in the technical field of.

[0012]

In order to achieve the above-mentioned object, the present invention provides (1) rose, Naniwaibara, Yaesaku
La, yoshino cherry, peach, plum, snow willow, southern
Mosquito, camellia, camellia, azalea, hirado azalea, yama
Azalea, Safflower dogwood, Scutellaria barbata, Kin
Gypsophila, daisies, dandelions, wisteria, white clover,
Astragalus, Kiri, Musikari, Forsythia, stock,
Hollyhocks, mallows, starfishes, sardines, hydrangeas,
Evening primrose, bindweed, morning glory, gardenia, kyo
A mucopolysaccharide fragmentation inhibitor comprising, as an active ingredient, one or two or more species selected from the group of various plant extracts extracted from the petals of plants of oleander and orchid .

(2) Rose, Naniwaibara, Yae Sakura,
Yoshino cherry tree, peach, plum, snow willow, sasanqua, mosquito
Camellia, camellia, hirado azalea, azalea, safflower
Dogwood, White clover, Hydrangea, Pineapple
An active oxygen scavenger exhibiting a superoxide dismutase-like action, which contains, as an active ingredient, one or more selected from various plant extract groups extracted from moss, bindweed, oleander and orchid .

(3) One or more selected from the group consisting of the mucopolysaccharide fragmentation inhibitor and the active oxygen scavenger described in (1) and (2) is contained as an active ingredient. And cosmetics.

The "mucopolysaccharide fragmentation inhibitor", "active oxygen scavenger" and "cosmetics" according to the present invention contain one or more kinds of various plant petal extracts as an active ingredient. There is. The various extracts of plant petals refer to extracts extracted from various plant petals with water, a lower alcohol or a lower alcohol aqueous solution. The term "lower alcohol" as used herein refers to alcohols that are methanol, ethanol and propanol and can be mixed with water at an arbitrary ratio.

The extract of lower petals of plant petals referred to in this specification was obtained using a Soxhlet extractor. As for the extract with water or lower alcohol aqueous solution, each sample was obtained by immersing a predetermined amount of a sample under a predetermined condition with a predetermined amount of water or lower alcohol aqueous solution. Then, various extracts of plant petals were concentrated by distilling off the respective organic solvents for the lower alcohol extract and the lower alcohol aqueous solution, and concentrating the extract under reduced pressure for the water extract, followed by freeze-drying. To obtain each plant extract.

First, the "mucopolysaccharide fragmentation inhibitor" according to the present invention will be described.

The "mucopolysaccharide" referred to in the present invention is a concept including all mucopolysaccharides of neutral mucopolysaccharides and acidic mucopolysaccharides, and particularly mucopolysaccharides related to humans, that is, hyaluronic acid, chondroitin, chondroitin. Needless to say, 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, heparan sulfate, pepperin and keratan sulfate I and keratan sulfate II are included.

The "mucopolysaccharide fragmentation inhibitor" according to the present invention has industrial applicability from the following viewpoints. That is, mucopolysaccharides (such as hyaluronic acid), which are the main constituents in the dermis of human skin, are considered to be important components for maintaining firmness of the skin. It is considered that this mucopolysaccharide loses its original function by being fragmented into low molecular weight by ultraviolet rays and active oxygen generated in the living body. Therefore,
Preventing fragmentation of this mucopolysaccharide (such as hyaluronic acid) is considered to be effective in preventing aging, including preventing wrinkles in human skin. Furthermore, the “mucopolysaccharide fragmentation inhibitor” according to the present invention opens up a way of application in the industrial field of medicine.

Various active ingredients in the "mucopolysaccharide fragmentation inhibitor" according to the present invention (various plant extracts and / or
Further, the compounding amount (content) of various compounds) is not particularly limited because it can be varied depending on the kind of the above-mentioned active ingredient and the combination thereof and the purpose of use, embodiment, use form, number of times of use and the like. In principle, an effective amount will suffice, but generally 0.0001 to 100% by weight, preferably 0.01 to 10% by weight, based on the composition of the mucopolysaccharide fragmentation inhibitor can be used. Furthermore, the active ingredient (various extracts of plants) according to the present invention can exert the action even with one kind, but by appropriately combining two or more kinds of active ingredients, an excellent synergistic effect is exhibited. be able to.

The mucopolysaccharide fragmentation-inhibiting effect of various plant extracts according to the present invention was measured by a known method (Kouyoko et al., Journal of Cosmetic Technology, Vol. 27 (2) p130 (199).
3)). That is, active oxygen is generated in the ascorbic acid-iron system or hydrogen peroxide-iron system to cause a fragmentation reaction of the mucopolysaccharide, and the residual mucopolysaccharide is measured by forming a complex with albumin, which is effective. We searched for the presence or absence of ingredients. Table 1 shows the measurement results of the water extract of each plant, Table 2 shows the measurement results of the lower alcohol aqueous solution (50% ethanol aqueous solution), and Table 3 shows the measurement results of the lower alcohol (99.5% ethanol) extract. Shows.

The inventor of the present invention can use a plant extract having a fragmentation inhibition rate of 30% or more in Tables 1, 2, and 3 as a "mucopolysaccharide fragmentation inhibitor" according to the present invention. Certified as an extract. That is, as the plant extract group, rose, Naniwaibara, Yae Sakura, Somei Yoshino, Peach, Ume, Yukiyanagi, Sasanqua, Kantsubaki, Camellia, Azalea, Hirado azalea, Yamatsuji, Kobushi, Hakumokuren, Peonies, Kanetion, Benibanhaohanohanohanohanohanohanoha , Snapdragon, daisies, dandelions, wisteria, clover, astragalus,
From the petals of various plants extracted from the plants of Kiri, Musikari, Forsythia, Chinese cabbage, stock, hollyhock, mallow, starfish, hydrangea, hydrangea, evening primrose, bindweed, noopia, gardenia, crape myrtle, oleander, orchid A mucopolysaccharide fragmentation inhibitor comprising one or more selected types as an active ingredient.

Next, the "active oxygen scavenger" according to the present invention will be described.

The "active oxygen scavenger" according to the present invention is
It has industrial utility from the following viewpoints. That is, it is known that active oxygen generated in the living body causes aging and diseases. In vivo, it can be eliminated by an enzyme such as superoxide dismutase (SOD). However, it is considered that such scavenging ability of active oxygen declines with aging and accelerates aging and diseases. Further, the SOD is a high molecular weight protein, and its activity is rapidly deactivated, which is not practical. On the other hand, the active oxygen scavenger according to the present invention is present in the extract of each plant, and some of them have an extremely excellent active oxygen scavenging ability and have stable characteristics. In particular, the plant extract is a complex, and has remarkable properties that it acts on various active oxygen species in a well-balanced manner and is excellent in stability against heat and the like. Furthermore, the “active oxygen scavenger” according to the present invention opens up the way of use in the industrial fields of medicine and food.

The blending amount (content) of various active ingredients (various extracts of plants and / or various compounds) in the "active oxygen scavenger" according to the present invention is the kind of the above-mentioned active ingredients and the combination thereof and the purpose of use thereof. There is no particular limitation as it can be changed according to the embodiment, the usage form, the number of times of use, and the like. In principle, an effective amount will suffice, but generally 0.0001 to 100% by weight, preferably 0.01 to 10% by weight, based on the active oxygen scavenger composition, can be used. Furthermore, the active ingredient (various extracts of plants) according to the present invention can exert the action even with one kind, but by appropriately combining two or more kinds of active ingredients, an excellent synergistic effect is exhibited. be able to.

The active oxygen scavenging action of various plant extracts according to the present invention was measured by a known method. That is, a phosphate buffer containing xanthine and nitroblue tetrazolium is reacted with xanthine oxidase under predetermined conditions to stop the reaction with sodium dodecyl sulfate, and then the absorbance at 560 nm is measured to search for the presence or absence of each active ingredient. It was The specific measuring method and the method of calculating the active oxygen scavenging rate will be described in detail in the section of Examples.

Table 4 shows the results of measuring the active oxygen scavenging rate (%) of various plant extracts according to the present invention. The inventor has found that the active oxygen scavenging rate in Table 4 is 30%.
The plant extracts shown above were identified as plant extracts that can be used as the “active oxygen scavenger” according to the present invention. That is, as the plant extract group, rose, Naniwaibara, Yae Sakura, Somei Yoshino, peach, plum, snow willow, sasanqua, citrus camellia, camellia, Hirado azalea, Yamatsuji, Kobushi, Hakukuren, Peonies,
Contains 1 or 2 or more kinds selected from various plant extracts extracted from petals of plants consisting of Safflower dogwood, white clover, Chinese cabbage, Chinese cabbage, hydrangea, evening primrose, bindweed, crape myrtle, orchid, orchid as an active ingredient An active oxygen scavenger exhibiting a superoxide dismutase-like action, which is characterized in that

Furthermore, "cosmetics" according to the present invention
Is considered to have industrial utility value from the following viewpoints. That is, as described above, the composition is composed of the cosmetics containing one or more of the "mucopolysaccharide fragmentation inhibitor" and the "active oxygen scavenger" as an active ingredient, and thus the plant to be incorporated into the cosmetics. The action and effect can be achieved based on the type of various petal extracts. Therefore, the cosmetic according to the present invention provides a skin anti-aging cosmetic as a basic effect.

The blending amount of various active ingredients according to the present invention with respect to various cosmetics may be varied depending on the types of the active ingredients and combinations thereof, the purpose of the cosmetic, the embodiment, the usage of the cosmetic, and the like. There is no particular limitation as it can be done. In principle, an effective amount should be present, but generally 0.0001 to 100% by weight of the cosmetic composition can be used, preferably 0.01 to 10% by weight, among which
The optimum range is 0.1 to 5.0% by weight. In particular, cosmetics and the like prepared at the time of use of the powder include the mucopolysaccharide fragmentation inhibitor, the active oxygen scavenger, and / or the antioxidant of the present invention.
It will be used at a high compounding rate including 0% by weight.

Furthermore, the active ingredient according to the present invention can exert the action and effect even with one kind selected from the group of various extracts of plant petals, but two or more kinds of active ingredients are appropriately combined and used. Thereby, an excellent synergistic effect can be achieved.

The application range of the cosmetic material according to the present invention is not particularly limited. That is, the present invention can be applied to all cosmetics that can utilize each action and effect according to the action and effect of the active ingredient of the present invention.

For example, one of the active ingredients according to the present invention
Mix two or more kinds into various cosmetic bases,
Various basic cosmetics such as creams, milky lotions, lotions, packs, facial cleansers, makeups such as foundations, blushers, lipsticks and white powders, hair wash, hair nourishing agents, shampoos, rinses and other various hair cosmetics, soaps It can be applied to a wide range of other cosmetics such as nail polish, cologne, etc. The embodiments of the various cosmetics can be applied in various modes such as a solution, emulsion, ointment, oil, wax, sol, gel, powder and spray.

The various extracts of each plant petal according to the present invention are all composites and act on a variety of active oxygen in a well-balanced manner, and also have good thermal stability and provide a highly safe cosmetic composition. It has the outstanding property of being able to.

[0034]

The various extracts of plant petals according to the present invention have a function of suppressing mucopolysaccharide fragmentation due to active oxygen, and are highly safe and stable "mucopolysaccharide fragmentation inhibitor"
Can be provided, and thus anti-aging of the skin can be achieved.

Further, various extracts of plant petals according to the present invention can provide an "active oxygen scavenger" which has a superoxide dismutase-like active oxygen scavenging action and is highly safe and stable. Therefore, it is possible to prevent skin aging.

[0036]

EXAMPLES Next, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples. In addition, the part in an Example shows a weight part unless there is particular notice.

(1) Preparation of plant petals As a raw material for plant petal extracts, dried and pulverized products of each plant petal were used. Put 2 to 5 g of the raw material into a cylindrical filter paper,
It was soaked in 50 to 100 ml of ion-exchanged water and heated and extracted at 60 ° C. for 8 hours to obtain a filtrate. This operation was repeated 3 times, and all the filtrates were combined and freeze-dried to obtain a water extract of the plant. Also, 50
For the% ethanol aqueous solution extract, 50% ethanol aqueous solution was used in place of water in the extraction operation of the extract. All extracts were combined, ethanol was distilled off as much as possible, and then freeze-dried to obtain a 50% ethanol aqueous solution extract of the plant. The ethanol extract of the plant was extracted with a Soxhlet extractor for 8 hours, the solvent was distilled off, and the extract was powdered to obtain an ethanol extract.

(2) Retrieval test of mucopolysaccharide fragmentation inhibitory action (A) Method for measuring hyaluronic acid fragmentation inhibitory action by active oxygen (ascorbic acid-iron system) 0.04% sodium hyaluronate (microbial origin) (Meiji Seika) 0.3M phosphate buffer (pH5.3) containing 0.45m
l, 0.05 ml of 1% aqueous solution of the sample and 20 mM ascorbic acid
0.025 ml and 0.025 ml of a 1 mM ferric chloride aqueous solution were added, and the mixture was incubated at 37 ° C for 24 hours, and then the reaction solution was added.
2 ml was taken out, 2.0 ml of 0.04 M sodium acetate / 0.08 M acetate buffer solution (pH 3.75) containing 0.1% albumin was added thereto, and the mixture was stirred well. After standing for 5 minutes, the turbidity of the produced complex of hyaluronic acid and albumin is measured as the absorbance (Esr) at 600 nm. (Amount of residual hyaluronic acid)

In the measurement of the amount of hyaluronic acid used in this method,
Since a complex may be formed between the sample and albumin, the turbidity when only sodium hyaluronate was removed in the above measurement operation as a blank, that is, the absorbance (Eb) was measured and corrected.

The original amount of hyaluronic acid used in the test sample is the turbidity of the complex with albumin when the fragmentation operation of hyaluronic acid in the ascorbic acid-iron system is omitted from the above measurement operations, that is, the absorbance ( Eso) was measured.

The fragmentation inhibition rate (%) of hyaluronic acid was calculated by the equation 1.

[Equation 1]

(B) Method for measuring inhibitory effect of hyaluronic acid fragmentation by active oxygen (hydrogen peroxide-iron system) 0.3M phosphate buffer solution containing 0.04% sodium hyaluronate (microbial origin) (Meiji Seika Co., Ltd.) pH5.3) 0.45m
In 1 l, 0.05 ml of 1% test sample aqueous solution and 100 mM hydrogen peroxide 0.0
Add 25 ml and 1 mM ferrous chloride aqueous solution 0.025 ml,
After incubating at 37 ℃ for 24 hours, 0.2 ml of the reaction solution
Remove and add 2.0 ml of 0.04M sodium acetate / 0.08M acetate buffer (pH3.75) containing 0.1% albumin,
Stir well. After standing for 5 minutes, the turbidity of the formed complex of hyaluronic acid and albumin was measured by measuring the absorbance at 600 nm (Es
r) is measured. (Amount of residual hyaluronic acid)

In the measurement of the amount of hyaluronic acid used in this method,
Since a complex may be formed between the sample and albumin, the turbidity when only sodium hyaluronate was removed in the above measurement operation as a blank, that is, the absorbance (Eb) was measured and corrected.

The original amount of hyaluronic acid used in the test sample is the turbidity of the complex with albumin when the fragmentation operation of hyaluronic acid in the ascorbic acid-iron system among the above-mentioned measurement operations is omitted, that is, the absorbance ( Eso) was measured. The fragmentation inhibition rate (%) of hyaluronic acid was calculated and calculated in the same manner as Equation 1. Table 1 shows the water extract of each plant, Table 2 shows the 50% ethanol extract of each plant, and Table 3 shows the result of hyaluronic acid fragmentation inhibitory action of the ethanol extract of each plant.

In Tables 1 to 3, "A
"sA-Fe system" indicates the results of the ascorbic acid-iron system,
"H ₂ O ₂ -Fe system" peroxide oxygen - shows the results of ferrous.

[Table 1]

[Table 2]

[Table 3]

From these results, water extracts of various plants,
A mucopolysaccharide fragmentation inhibitory effect is observed in the ethanol aqueous solution extract and the ethanol extract.

(3) Method for measuring active oxygen scavenging action First, the following coloring reagent, enzyme solution, blank solution and reaction stop solution are prepared. Color reagent: 0.14M phosphate buffer (pH 8.0) with xanthine 0.04 mmol / l, nitroblue tetrazolium (NO
_2- TB) is dissolved to be 0.24 mmol / l. Enzyme solution: Xanthine oxidase (derived from buttermilk) is dissolved in 0.1 M phosphate buffer (pH 8.0) at 0.049 unit / ml. Blank solution: 0.1 M phosphate buffer (pH 8.0) Reaction stopper: 69 mM sodium dodecyl sulfate

"Enzyme / Sample Sample (S)" is a 1% aqueous sample solution (aqueous solution of various plant extracts or compound)
To 0.1 ml, add 1.0 ml of color reagent solution and 1.0 ml of enzyme solution, heat at exactly 37 ° C for 20 minutes, and then add 2.0 ml of reaction stop solution to stop the reaction. After stopping the reaction, the absorbance (Es) at 560 nm is measured. In addition, the “enzyme sample (B)” is the same as the measurement of the “enzyme / sample sample” by adding 0.1 ml of distilled water instead of the 1% sample (aqueous various extract solution or compound aqueous solution of plant). And the absorbance (Eb) at 560 nm is measured.

On the other hand, "enzyme-free sample specimen (SB)" is 1
% Add 0.1 ml of color test solution and 1.0 ml of blank solution to 0.1 ml of sample aqueous solution (plant various extract aqueous solution or compound aqueous solution), and after heating exactly at 37 ° C for 20 minutes, add 2.0 ml of reaction stop solution to start the reaction. After stopping, the absorbance (Esb) at 560 nm is measured. The "enzyme-free sample (BB)" is the above-mentioned "enzyme-free sample sample" obtained by adding 0.1 ml of distilled water in place of a 1% sample (an aqueous solution of various extracts of plants or an aqueous solution of a compound).
The same operation is performed to measure the absorbance (Ebb) at 560 nm. The active oxygen scavenging rate (%) was used.

[Equation 2]

Table 4 shows the results of the active oxygen scavenging action of various extracts of each plant.

[Table 4] From these results, active oxygen scavenging action is recognized in various extracts of various plants. Next, examples of blending the antioxidant of the present invention with cosmetics will be shown. The mixing ratio is parts by weight.

[0051]

[Prescription example 1] lotion     <Composition> (wt%)   Active oxygen scavenger 0.2   Glycerin 5.0   Polyoxyethylene sorbitan monolaurate (20E.0) 1.5   Ethanol 10.0   Preservative Suitable amount   Fragrance suitable amount   Purified water balance [Production Method] The above raw materials are added to purified water and mixed uniformly.

[0052]

[Formulation Example 2] Cosmetic cream <Composition> (% by weight) Oily component: Beeswax 2.0 Stearyl alcohol 5.0 Stearic acid 8.0 Squalane 10.0 Emulsifier: Self-emulsifying glyceryl monostearate 3.0 Polyoxyethylene cetyl ether (20E.0) 1.0 Fragrance Proper amount Preservative Proper amount Aqueous phase: Active oxygen scavenger 0.2 Propylene glycol 5.0 Purified water Remainder [Production method] The raw materials for the aqueous phase are mixed and heated to 70 ° C to form an aqueous layer. On the other hand, mix the other ingredients, heat and dissolve
Use 70 ° C as the oil phase. This oil phase part was added to the above-mentioned water layer part to carry out preliminary emulsification, to homogenize the homomixer uniformly,
Cool to 30 ° C to obtain a cream.

[0053]

[Formulation Example 3] Emulsion <Composition> (wt%) Oil phase: Squalane 8.0 Vaseline 2.0 Beeswax 0.5 Emulsifier: Sorbitan sesquioleate 0.8 Polyoxyethylene oleyl ether (20E.0) 1.2 Aqueous phase: Active oxygen scavenger 0.1 Carboxyvinyl Polymer 0.2 Propylene glycol 0.5 Potassium hydroxide 0.1 Ethanol 7.0 Perfume Proper amount Preservative Proper amount Purified water Remainder [Production method] The raw materials for the above aqueous phase are mixed and heated to 70 ° C to form an aqueous layer. On the other hand, mix the other ingredients, heat and dissolve
Use 70 ° C as the oil phase. The oil phase is added to the above water layer to emulsify and then cooled to 30 ° C to obtain an emulsion.

[0054]

[Formulation Example 4] Packing agent <Composition> (wt%) Powder: Titanium oxide 8.0 Kaolin 7.0 Oil: Olive oil 3.0 Fragrance Suitable amount Preservative Suitable amount Water phase: Active oxygen scavenger 1.0 Vinyl acetate resin emulsion 15.0 Polyvinyl alcohol 10.0 Glycerin 5.0 Ethanol 5.0 Purified water The remainder [Production method] The raw materials of the aqueous phase are mixed and made uniform. Further, the other components are mixed and stirred until uniform.

[0055]

[Formulation Example 5] Cream foundation <Composition> (% by weight) Oil phase: Stearic acid 0.2 Glycerin monostearate 5.0 Cetanol 1.0 Propylene glycol monolaurate 3.0 Squalane 7.0 Cetyl octanoate 8.0 Pigment: Titanium oxide 8.0 Kaolin 5.0 Talc 2.0 Color pigment Proper amount Preservative Proper amount Perfume Proper amount Aqueous phase: Active oxygen scavenger 0.3 Triethanolamine 1.2 Solbit 3.0 Purified water Remainder [Production method] Mix pigment and grind. The aqueous phase is prepared, and the mixed pigment is added thereto to disperse the mixture, followed by heating to 75 ° C. Adjust the oil phase and heat to 80 ° C. The oil phase is added to the aqueous phase with stirring to emulsify and then cooled, and a fragrance is added at 50 ° C and further cooled to 30 ° C to obtain a creamy foundation.

[0056]

EFFECTS OF THE INVENTION Based on the characteristics of mucopolysaccharide fragmentation inhibitory action and active oxygen scavenging action possessed by various plant extracts and / or compounds, various aging and diseases caused by active oxygen can be effectively prevented. Furthermore, an excellent mucopolysaccharide fragmentation inhibitor and active oxygen scavenger can be provided based on these characteristics. Moreover, the mucopolysaccharide fragmentation inhibitor, active oxygen scavenger is a natural product contained in various extracts of plants, and since it is a complex, it is stable to heat, etc., and has few side effects, Not only cosmetics but also medical and food technical fields are widely opened, and the remarkable effects of achieving the object of the invention are achieved.

─────────────────────────────────────────────────── ─── Continued Front Page (51) Int.Cl. ⁷ Identification Code FI A61K 7/00 A61K 7/00 K W 7/06 7/06 7/48 7/48 A61P 39/06 A61P 39/06 43 / 00 107 43/00 107 (56) Reference JP-A-6-24937 (JP, A) JP-A-4-5237 (JP, A) JP-A-5-238943 (JP, A) JP-A-7-126143 (JP, A) Electrophoresis, Vol. 10, No. 1, pp. 46-48 (1989) Fragrance Journal, Vol. 21, No. 11, pp. 70-79 (1993) (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 35/78-35/84 A61K ⁷ /00-7/50 BIOSIS (DIALOG) CA (STN) MEDLINE (STN)

Claims (3)

(57) [Claims] 1. Rose, Naniwaibara, Yae Sakura, Somei
Iyoshino, Peach, Ume, Snow Willow, Sasanqua, Kanz
Camellia, camellia, azalea, hirado azalea, azalea, azalea
Dogwood, dogwood, snapdragon, snapdragon,
Daisies, dandelions, wisteria, white clover, astragalus
Cormorant, Kiri, Musikari, Forsythia, Stock, Tachiao
Lee, mallow, starfish, sardine, hydrangea, matsuyoi
Gusa, Convolvulus, Noosa Morning Glory, Gardenia, Kyo Chikuto
A mucopolysaccharide fragmentation inhibitor comprising, as an active ingredient, one or more selected from various plant extract groups extracted from the petals of each plant consisting of c and orchid . 2. Roses, Naniwaibara, Yaesakura, Someiyoshino, Peach, Ume, Yukiyanagi, Sasanqua, Camellia, Camellia, Camellia, Hiradotsuji, Yamatsuji, Safflower, Dogwood, White clover, Hydrangea, each of the flowers of Matsuiyugusa, Hirugagao, Ranunculus japonicus, Kyokuto, Kyokuto, Kyokuto. An active oxygen scavenger exhibiting a superoxide dismutase-like action, which comprises, as an active ingredient, one or more selected from various extracted plant extracts. 3. An active ingredient comprising one or more selected from the group consisting of the mucopolysaccharide fragmentation inhibitor according to claim 1 and the active oxygen scavenger. Cosmetics to do.