JP3432175B2 - Cell growth promotion cosmetics - Google Patents

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a cosmetic having an action of promoting proliferation of epidermal cells and preventing skin aging.

More specifically, the present invention relates to a cell growth promoting cosmetic composition which is effective in preventing and improving wrinkles, creases, dry skin and spots caused by aging of the skin, and is excellent in safety.

[0003]

2. Description of the Related Art Human skin gradually ages with aging, and it is known that various external stimuli other than aging cause skin aging. In particular, ultraviolet rays in the sunlight are collagen, which is a constituent of extracellular matrix,
It causes cross-linking or cutting of elastin and the like, thereby reducing elasticity and moisturizing property of the skin and causing wrinkles, dry skin, and rough skin. The skin that has undergone such changes is more susceptible to external stimuli, cell activity is reduced, and the regenerative capacity of the extracellular matrix is reduced, leading to a vicious circle and aging rapidly progresses. The disappearance of luster of the skin, the increase of spots, dullness, etc., which progresses in a short period of time, is often due to the strong external stimulus as described above.

Therefore, in order to prevent skin aging due to external stimuli, ultraviolet absorbers and moisturizers have been incorporated into cosmetics. In addition, cosmetics may be mixed with components that inhibit collagenase or elastase to maintain the extracellular matrix in a normal state, or components such as vitamin C and vitamin E that have a cell growth promoting action may be mixed. Came.

However, it is difficult to completely block harmful ultraviolet rays with an ultraviolet absorber in an amount that can be usually compounded, and if it is intentionally compounded in a large amount, irritation to the skin becomes a problem. It is also difficult to sufficiently activate skin cells with a moisturizer, a collagenase inhibitor, etc., and a cell growth promoting component such as vitamin C has problems in safety, stability, etc., and it was not always satisfactory.

[0006]

SUMMARY OF THE INVENTION An object of the present invention is to provide a cosmetic having a cell proliferation promoting effect which is effective in preventing and improving undesired changes associated with aging of the skin such as wrinkles, age spots, dullness, and dry skin. To provide a fee.

Another object of the present invention is to provide a cosmetic composition containing a highly safe ingredient derived from a natural product and imparting a preventive / ameliorating action to skin aging.

[0008]

[Means for Solving the Problems] The cell growth promoting cosmetic of the present invention which has succeeded in achieving the above-mentioned object is a soybean extract, glabridin, fruit extract, sardine extract, nin
It is characterized by containing a gin extract and a safflower extract and having a cell growth promoting action by a synergistic action of these plural components.

[0009]

Here, the "fruit extract" includes not only an extract obtained by subjecting fruits to an extraction treatment with a solvent, but also juice obtained by squeezing juicy fruits.

[0011] "Ougon extract" is the Ougon (S
cutellaria baicalensis Ge
orgi. ), The "carrot extract" is carrot (Panax ginseng CA Meyer)
The root of "Safflower extract" is safflower (Cartha
mus tinctorius L. 2) flowers are extracted with hydrous alcohols such as hydrous ethanol and hydrous 1,3-butylene glycol.

The "cell growth promoting action" is an action confirmed by the test method described below.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION The soybean extract used in the present invention is
Soybean (Glycine max Merrill) with hydrous alcohol having an alcohol concentration of about 30 to 70% by weight (for example, hydrous ethanol, hydrous 1,3-butylene glycol, hydrous propylene glycol, hydrous glycerin, etc.)
Is a saponin-containing extract obtained by subjecting the above to extraction treatment. In the case of an extract solution with a solvent that can be mixed in the cosmetic composition, the extract solution can be blended in the cosmetic composition as it is, or as concentrated or diluted as necessary. The extract may be once made into a dry solid and then redissolved to be used for cosmetic production.

Glabridin is a licorice (Glycyrrh)
iza glabra L. ) Is a kind of flavonoids contained in the root. For this compound,
It is known to have a whitening effect, an antioxidant effect and an antibacterial effect. This extract may be used as it is, since it is contained in a large amount in an extract (so-called oil-soluble licorice extract) obtained by extracting licorice root with ethanol, acetone, ethyl acetate or the like.

As the fruit extract used in combination with the above-mentioned two components, those obtained from many fruits such as citrus fruits and the like can be used. Specific examples of suitable fruit fruits are orange, lemon, In addition to citrus fruits such as lime, grapefruit, mandarin orange and yuzu, jujube, hawthorn, grape, apple, strawberry, etc. are available.

It is suitable to use an extract solution with a hydrophilic solvent for fruits such as jujube and hawthorn fruits for which it is difficult to extract the juice component, but in the case of juicy fruits such as citrus fruits, You may use the juice obtained by the soup. As the solvent used for extraction, the same hydrous alcohol as the solvent used for producing the soybean extract is suitable.

The cell growth promoting action of the cosmetic composition of the present invention appears for the first time when soybean extract, glabridin and a certain fruit extract are used in combination. In this case, it has been empirically found that many fruit extracts effective for expressing a cell growth promoting action are those derived from fruits having a high content of organic acids and sugars such as the fruits exemplified above. However, the requirements for the fruit extract for the onset of effects have not been completely elucidated. Therefore,
When a fruit extract for carrying out the present invention is to be selected from those other than those exemplified above (or when one other than those exemplified above is to be used in combination), soybean extract and glabridin It is desirable to confirm the effectiveness based on the result of the experiment for confirming the cell growth promoting action when used in combination, and then select.

The combined use of a plurality of fruit extracts is often effective in enhancing the activity and is therefore preferable.

The preferable combination ratio (weight ratio) of the above-mentioned active ingredients in the cosmetic of the present invention is 10 graburidine.
When 0 is set, it is as follows. Soybean extract: 100-5,000 as solid content Fruit extract: 10-1,000 as solid content For cosmetics, the total amount of these three components is 0.001
It is desirable to add it so as to be about 2.0% by weight.

When the extract of Scutellariae sinensis extract, the extract of carrot or the extract of safflower and / or safflower is also added, the addition ratio of these extracts is about 0.
It is desirable that the content be 0001 to 0.5% by weight.

The cell growth promoting cosmetic of the present invention takes the form of any cosmetic applied to the skin, such as lotion, milky lotion, cream, pack, gel cosmetic, powder cosmetic, soap, liquid detergent and the like. be able to.

In the case of producing the cosmetic of the present invention, the constituents usually used for producing the cosmetic, such as oil, surfactant, thickener, humectant, preservative, perfume, colorant and chelating agent. , An amino acid, a saccharide, etc., to which an active ingredient specific to the present invention is added, and if necessary, any physiologically active substance such as glycyrrhizic acid and a salt thereof, which is an anti-inflammatory agent, glycyrrhetinic acid and a derivative thereof (stearyl glycyrrhetinate, etc.), allantoin , Azulene, baicalin, baicalein, horse chestnut extract, sardine extract, lauren extract, etc .; whitening agents such as ascorbic acid, magnesium ascorbate phosphate, ellagic acid, arbutin, kojic acid, placenta extract, mulberry bark extract , Ginseng extract,
Produced by a conventional method by mixing waremoko extract, age extract, etc .; vitamins, antioxidant tocopherol and its derivative, natural vitamin E; animal ingredients collagen, elastin, hyaluronic acid, chondroitin sulfate, etc. be able to.

[0023]

The present invention will be described below with reference to examples. The components specific to the present invention used in each example are as follows.

Soybean Extract: Water-containing 1,3-butylene glycol extract of crushed soybean (solid concentration 0.35% by weight) Glabridine: Licorice ethanol extract containing 40% by weight of glabridin Jujube extract: jujube fruit Water-containing 1,3-butylene glycol extract (solid content concentration 5.5%) Hawthorn extract: Water-containing 1,3-butylene glycol extract of hawthorn fruit (solid content concentration 1.0%)

Grapefruit extract: 50% 1,3-butylene glycol aqueous solution 1 kg per 1 kg of roughly crushed raw material fruit
An extract obtained by adding 0 kg and allowing the mixture to stand for 7 days and then filtering. Solid content concentration 2.3%.

Apple extract: Extract extracted in the same manner as the grapefruit extract. Solid content concentration 1.8%.

Orange juice: 1 kg of roughly crushed raw material fruit is squeezed with a squeezing type squeezing machine to obtain a juice, and 10 kg of a 50% 1,3-butylene glycol aqueous solution for preserving the juice.
What was obtained by adding and filtering. Solid content concentration 1.8%.

Lemon juice: obtained in the same manner as the above orange juice. Solid content concentration 2.1% Lime juice: Obtained in the same manner as the above orange juice. Solids concentration 1.7% Yuzu fruit juice: obtained in the same manner as the above orange juice. Solid content concentration 1.5% Grape juice: obtained in the same manner as the above orange juice. Solid content concentration 2.3% Strawberry juice: obtained in the same manner as the above orange juice. Solid content concentration 1.4%

Examples 1 to 4, Comparative Examples 1 to 3 Using the following A to C as raw materials, lotions were produced by a conventional method. (A) Dipropylene glycol 4% by weight 1,3-butylene glycol 2 Polyoxyethylene (60) hydrogenated castor oil 1 ethyl alcohol 4 methyl paraoxybenzoate 0.1 citric acid 0.2 sodium citrate 0.1 perfume 0.1. 02 (B) See Table 1 (C) Water balance

[0030]

[Table 1] Component B Example 1: Soybean extract (0.105%), glabridin (0.01%), jujube extract (0.055%), lime juice (0.017%) Example 2: Ingredient of Example 1 + safflower extract (0.03
%) Example 3: Soybean extract (0.0625%), glabridin (0.005%), orange juice (0.0018)
%), Lemon juice (0.0021%), grapefruit juice (0.0023%), apple juice (0.0018)
%) Example 4: Components of Example 3 + carrot extract (0.00
65%), sardine extract (0.012%) Comparative Example 1: Excluding glabridin from the components of Example 1 Comparative Example 2: Excluding the soybean extract from the components of Example 1 Comparative Example 3: Components of Example 1 More jujube extract and lime juice were excluded (Note: the above licorice extract was actually used as glabridin, but the indicated addition rate is the addition rate as glabridin. The same applies to each of the following examples.)

Test Example 1 The lotions of Examples 1 to 4 and Comparative Examples 1 to 3 were tested for their cell growth promoting action by the following MTT method.

MTT method (J. Immunol. Meth
od 93, 157, 1986): Precultured human normal newborn dermal fibroblasts (NB1RGB) were dispensed into a 96-well microplate, and the sample was lysed 10% FB
RITC80-7 medium supplemented with S culture solution (manufactured by Kyokuto Pharmaceutical Co.,
pH 7.2) is added and the mixture is cultured at 37 ° C. for 3 days under 5% CO ₂ . Then, MTT reagent (3- (4,5-dim
thy1-2-thiazolly1) -2,5-dip
heny1-2H tetrazolium bromi
de, 5 mg / ml) and then incubated for 4.5 hours (reactive oxygen from mitochondria in the grown cells reacts with the MTT reagent, and the color of the reagent which was yellow has an absorption peak at 570 nm. It turns blue-yellow.). Then, the absorbance (A) at 570 nm is measured using a microplate reader. Separately, the same culture is performed without adding the sample, and the absorbance (B) is measured. Further, the absorbance (C) in the case where the same treatment is carried out without adding the sample and the cells is measured, and the absorbance (D) in the case where the same treatment is carried out without adding the sample and the cells is also measured. For each absorbance measurement value, the absorbance at 650 nm measured at the same time is subtracted to correct the effect of increased turbidity due to the grown cells. From each corrected absorbance, the cell growth promotion rate is calculated by the following formula.

Cell growth promoting rate (%) = [{(A-C)-
(BD)} / (BD)] × 100

The results are shown in Table 2, and it was confirmed that the lotions of all Examples have a cell growth promoting action, and particularly the lotions of Examples 2 and 4 have an extremely excellent cell growth promoting action. . However, the lotions of Comparative Examples 1 to 3 were all ineffective.

[0035]

[Table 2]

Example 5 The following raw materials were processed by a conventional method to produce a cream.   Cell growth promoter       Soybean extract 0.35% by weight       Grabridine 0.03       Carrot extract 0.065       Scutellaria extract 0.012       Safflower extract 0.0075       Orange juice 0.09       Jujube extract 0.055       Grapefruit extract 0.023   Cetostearyl alcohol 3.5   Squalane 30.0   Beeswax 3.0   Reduced lanolin 5.0   Stearyl paraoxybenzoate 0.1   Polyoxyethylene (20) sorbitan monostearate 2.0   Glycerin monostearate 2.0   1,3-butylene glycol 5.0   Fragrance 0.05   Purified water balance

Comparative Example 4 A cream was produced by removing 8 kinds of cell growth promoting substances from the raw material of Example 5.

Test Example 2 18 women (age 3) who complain of dullness, wrinkles, and dryness of the skin
3 to 45 years old; the following evaluation tests were carried out on the creams of Example 5 and Comparative Example 4 with the panelists having the following two criteria and scores of 1 and 2 for both items.

Test method: The panelists were divided into two groups of 9 persons, one of which was the cream of Example 5 and the other of which was the cream of Comparative Example 4 for 50 days each twice a day in the morning and evening.
It was applied to the face. After 50 days, the number and depth of gloss, wrinkles and dryness, and wrinkles on the coated surface were observed with the naked eye, and answers were given according to the following criteria.

[Gloss of skin, dullness and dryness] Observation result 1 of the score: No dullness of the skin and strong dullness. Many exfoliations of the stratum corneum are observed. 2 There is no gloss on the skin, there is dullness on the whole, and exfoliation of the stratum corneum can be seen here and there. 3 There is slight gloss on the skin and no dullness is visible. The stratum corneum is not peeled off with the naked eye, but it is peeled off when lightly rubbed with a cotton swab. 4 The skin is shiny and firm. Almost no exfoliation of the stratum corneum even with rubbing with makeup cotton.

[Wrinkles] Observation result of score 1 There are many wrinkles on the outer corners of the eyes and many wrinkles are observed . Wrinkles around the 2nd corner are seen, and the number is slightly large. 3 Wrinkles around the outer corner of the eye are observed, but the number is small. 4 Wrinkles around the corners of the eyes are not recognized under normal facial expression.

The test results are shown in Tables 3 and 4.

[0043]

[Table 3]

[0044]

[Table 4]

Example 6 An emulsion having the following composition was produced by a conventional method. Soybean extract 0.0625% by weight Grabridine 0.02 Lemon juice 0.0042 Jujube extract 0.0055 Yuzu juice 0.006 Hawthorn extract 0.02 Carrot extract 0.065 Cetyl alcohol 0.5 Squalane 3.0 Beeswax 1.0 Vaseline 2.0 Polyoxyethylene (20) sorbitan monooleate 1.0 Glycerin monostearate 1.0 Dipropylene glycol 4.0 Ethanol 2.0 Methyl paraoxybenzoate 0.12 Sodium hyaluronate 0.1 Purified water balance

Example 7 A pack having the following composition was produced by a conventional method. Soybean extract 0.07% by weight Grabridine 0.03 Scutellaria extract 0.05 Safflower extract 0.08 Grapefruit juice 0.008 Grape juice 0.003 Strawberry juice 0.008 Polyvinyl alcohol 15.0 Polyethylene glycol 3.0 Propylene glycol 7.0 Ethanol 10.0 Methylparaben 0.05 Fragrance 0.05 Purified water balance

Example 8 An ointment having the following composition was produced by a conventional method. Soybean extract 0.05% by weight Grabridine 0.02 Carrot extract 0.03 Lime juice 0.0055 Lemon juice 0.008 Orange juice 0.005 Jujube extract 0.003 Cetyl alcohol 18.0 Mokuro 20.0 Polyoxyethylene (20) monooleate 0.25 Glycerin monostearate 0.25 Vaseline 40.0 Purified water balance Made

─────────────────────────────────────────────────── --Continued from the front page (56) References JP 62-36304 (JP, A) JP 59-93011 (JP, A) JP 9-249576 (JP, A) JP 8- 143442 (JP, A) JP-A 5-320061 (JP, A) JP-A 10-287526 (JP, A) JP-A 10-182411 (JP, A) JP-A 9-291011 (JP, A) JP-A-10-152428 (JP, A) (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K ^7/ 00-7/50

Claims (2)

(57) [Claims] 1. A soybean extract, glabridin, fruit extract, sardine extract, carrot extract and safflower extract.
A cell growth-promoting cosmetic material which contains exudates and thereby has a cell growth promoting action. 2. The cell according to claim 1, wherein the fruit extract is an extract of one or more fruits selected from the group consisting of citrus fruits, jujube, hawthorn, grape, apple and strawberry.
Growth promoting cosmetics.