JP3382145B2 - External preparation for skin - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [0001] BACKGROUND OF THE INVENTION 1. Field of the Invention
Skin fibroblast activation is synergistically enhanced and dermal fibers
Protects blast cells from UV damage
Skin aging symptoms such as wrinkles, reduced skin elasticity
Effective for prevention or improvement of anti-inflammatory activity, promotion of wound healing
The present invention relates to an external preparation for skin having an action. For more information,
Agaricus mushroom (Agaricus blazei (Millill) mycelium extract
And one or two selected from mycelium culture filtrate (hereinafter referred to as
Agaricus mushroom extract) and alkali hydroxide solution
A mixture of sugars isomerized by acting (hereinafter, isomerized sugar mixture)
Abbreviated as above). [0002] 2. Description of the Related Art It is caused by external stress such as aging and ultraviolet rays.
Aging of the skin such as wrinkles, spots, and decreased skin elasticity
Symptoms include dermal fibroblast dysfunction and matrix
An important factor is the reduction or degradation of fiber.
Therefore, it has anti-aging and anti-aging properties, which have the effect of preventing and improving skin aging.
Activation or proliferation of fibroblasts to obtain a skin external preparation
Attempts have been made to search for and formulate components having a potent action. [0003] For example, loquat extract (Japanese Patent Publication No. 5-1720)
No. 6), α-hydroxyacetic acid (Japanese Unexamined Patent Publication No.
No. 22), sterol esters of α-hydroxy acids
(JP-A-8-104632), 6-benzylamino
Pudding (Japanese Patent Application Laid-Open No. Hei 7-233037),
Nuclease (JP-A-7-309778);
Syl-L-glycyl-L-histidine (JP-A-7-31619)
No. 2), milk-derived fibroblast growth factor (Japanese Unexamined Patent Publication No.
No. 119867), oxidized coenzyme A (JP-A-Hei.
8-175961) and the like. However, the above-mentioned dermal fibroblast activation
Some of the active ingredients have insufficient effects.
Instability in the skin base for external use.
If present, contain a significant amount to obtain an effective effect
There were also things that had to be done. Also preferred
Has unfavorable side effects and irritation, and is stable
To external preparations in terms of smell and color.
Difficult to maintain, consistent action and quality
There were many difficult things. [0005] Therefore, in the present invention,
The synergistic effect of moisturizing and dermal fibroblast activation
Increased, resulting in wrinkles and spots, decreased skin elasticity
Excellent effect in preventing or improving skin aging symptoms
The aim was to obtain an external preparation for skin. [0006] SUMMARY OF THE INVENTION The present inventor has proposed an agaricus.
A high dermal fibroblast activating effect was obtained with mushroom extract
(Japanese Patent Application No. 9-31277)
1). This time, this Agaricus mushroom extract and isomerized sugar mixture
Activate skin physiology by using in combination with
Synergistic enhancement of moisturizing and fibroblast activating effects
And use them in combination in a skin external preparation.
This can cause wrinkles and spots, decrease skin elasticity,
Excellent effect for preventing or improving skin aging symptoms
A topical skin preparation that can be used was obtained,
I came to a decision. [0007] Embodiments of the present invention will be described. [0008] Agaricus mushroom (Agaricu
s blazei Murill) is a basidiomycete agaricaceae
It is a kind of Ratake mushroom, and is also known as Kawariharatake and Himematsutake
Called, deposited number at Institute of Biotechnology and Industrial Technology, National Institute of Advanced Industrial Science and Technology
No. 4731, deposited by Life Science Bacteria. Agaricus for obtaining Agaricus mushroom mycelium
As a method for cultivating Mushroom strains, it is generally used for cultivating Basidiomycetes.
Either solid culture method or liquid culture method
However, the latter method is preferably used in terms of productivity.
You. The culture medium used for cultivation of Agaricus mushrooms
It is only necessary to contain nutrients necessary for growth,
Is better. That is, as a carbon source, for example, glucose
, Sucrose, maltose, starch, etc.
Any carbon source can be used. As a nitrogen source, for example,
Ammonium sulfate, ammonium nitrate, urea, etc.
Natural complex nutrients include potato extract and d.
Carrot extract, malt extract, peptone, koji extract,
Yeast extract, yeast powder, etc. can be used.
Trace elements such as inorganic salts and vitamins required
Used. Culturing is usually performed under aerobic conditions.
The shaking culture method or the aeration stirring culture method is used. culture
Shaking during reciprocating or rotary shaking every 24 hours for several minutes
However, continuous shaking may be used. The culture temperature is
15 ° C to 40 ° C, preferably around 20 ° C to 30 ° C
You. The pH of the medium is preferably in the range of 3.0 to 9.0.
The growth is good at 5 to 7.0. Also, during culture, illuminate
It is preferable that there is no illumination, but about 11 to 14 hours a day
Is possible. [0011] The number of days of culture depends on the cultivation of the physical environment, medium composition, etc.
Depending on the nutrient conditions, the growth of mycelium is sufficient.
For a period of 2 to 120 days, especially preferred.
Or 5 to 90 days, when the maximum mycelium is produced
Good. After completion of the culture, the culture is centrifuged or filtered.
This separates the mycelium from the culture filtrate. Centrifugation
100-5000G, preferably 800-3000G
It can be performed by a centrifugal operation giving gravitational acceleration.
In addition, filtration is 3.5 to 200 mesh, particularly preferred.
Using a 4-16 mesh membrane filter
And filter. A place where an extract is obtained from the mycelium of Agaricus mushroom
The raw mycelium obtained from the culture solution cultured as described above.
Can be used as it is or after drying. Mycelium
As a solvent for extraction from the body, a polar solvent is preferably used.
It is. For example, water, ethanol, methanol, isopro
Panol, isobutanol, n-hexanol, methyl alcohol
Mil alcohol, 2-ethylbutanol, n-octylal
Alcohols such as coal, glycerin, ethylene glycol
, Ethylene glycol monomethyl ether, ethyl
Glycol monoethyl ether, propylene glycol
Propylene glycol monomethyl ether, propylene
Len glycol monoethyl ether, triethylene glycol
Coal, 1,3-butylene glycol, hexylene glycol
Selected from polyhydric alcohols such as
One solvent or a mixture of two or more solvents can be used. Ma
Addition of inorganic salts, surfactants, etc. to polar solvents
May be. Among these polar solvents, ethanol,
Selected from methanol, 1,3-butylene glycol and water
One solvent or a mixture of two or more solvents,
A solvent in which an inorganic salt and a surfactant are added to a medium is preferably used.
Can be Further, as an extraction method, cooling at room temperature or cooling or
Is a method of extracting by heating and impregnating, steam distillation
Extraction method using a distillation method, such as raw agaricus mushroom
Squeezing method of directly extracting the thread body to obtain an extract is exemplified,
Extract these methods alone or in combination of two or more
I do. During the extraction, the mycelium of Agaricus mushroom and the solvent
The ratio is not particularly limited, but Agaricus fungi
Solvent 0.5 to 1000 times by weight, especially extraction
It is preferably 0.5 to 100 times by weight in terms of operation and efficiency. Ma
The extraction temperature ranges from 5 ° C to the boiling point of the solvent at normal pressure.
The extraction time depends on the extraction temperature, etc.
But preferably between 2 hours and 2 weeks
No. The agaricus thus obtained is
The mushroom mycelium extract can be used as is
However, fractionation, deodorization, and
It can also be used after adding purification operations such as color and concentration. This
These extracts, their purified and fractionated products are
Can be removed to dryness.
Solubilized or suspended in a solvent such as purified water, or
Can be added to the skin external preparation in the form of an emulsion. On the other hand, the filtered mycelium culture filtrate is left as it is.
It can be used in skin external preparations.
Fractionation, deodorization, decolorization, concentration within the range that does not lose the effect of the invention
It can also be used by adding a purification operation such as these
The culture filtrate, its fractions, and purified products are freed of the solvent from them.
It can be dried to remove
Form solubilized or suspended in a solvent such as water production, or milk
It can be added to a skin external preparation in the form of a preparation. External preparation for skin of these Agaricus mushroom extracts
The amount added to the product depends on its effect, odor and color tone when added.
The concentration range should be 0.0001 to 5% by weight.
Is desirable. Alkali hydroxide used in the present invention
A mixture of sugars isomerized by the action of
Compound) is water such as sodium hydroxide or potassium hydroxide
Reaction product mixing of isomerized sugar with alkali oxide solution
Product, especially isomerizing glucose or lactose
Or a mixture thereof is preferably used.
These are described in JP-B-48-1504.
It can be manufactured by a known method. External preparation for skin
The content in the composition is 0.0001-3.0 weight
% Is appropriate. The external preparation for skin according to the present invention includes an external preparation base.
Oils and fats, waxes, hydrocarbons, and fats commonly used in cosmetics
Acids, lower alcohols, higher alcohols, polyhydric alcohols
Coals, esters, surfactants, water-soluble polymers, etc.
It can be contained. In addition, other skin cell activation
Agents, anti-inflammatory agents, active oxygen scavengers, whitening agents, moisturizers, ultraviolet
Can contain radiation absorbers, antiseptic / antifungal agents, fragrances, etc.
You. The external preparation for skin according to the present invention is a lotion.
In the form of preparations, emulsions, gels, creams, ointments, etc.
be able to. Also, lotion, milky lotion, cream, beauty
Skin cosmetics and makeup such as liquids, massage agents, packs, etc.
Up base lotion, makeup base cream
Makeup such as cream, liquid or cream foundation
Up cosmetics, hand cream, leg cream, body
To be provided as body cosmetics such as lotions
Can be. [0022] EXAMPLES Agaricus mushrooms used in Examples of the present invention
Production examples of kiss and isomerized sugar mixtures are shown below. [Production Example 1] Agaricus mushroom mycelium extract According to the method described in JP-B-61-47518, aga
Rix mushroom mycelium extract was obtained. That is, glucose 4
g, yeast extract 4 g, malt extract 10 g, and purified water 1
Liter of pH 5.5 medium at 120 ° C. for 20 minutes
Agaricus mushroom mycelium cultured on an agar medium
Seed and leave at 30 ° C for 30 days with occasional stirring
Precultured. Next, 20 g of glucose, 5 g of yeast powder, and
PH 5, consisting of 20 ppm of foaming agent and 1 liter of purified water.
2.0 liters of main culture medium at 120 ° C for 20 minutes
Then, 200 ml of the preculture was inoculated into this, and
The cells were cultured in a shaker. Stop culture after 30 days of culture
Then, the mycelium was separated and collected by centrifugation. On mycelium
Then, add 7 weight times of purified water and heat extract at 95 ° C for 2 hours.
Issued. The supernatant obtained by removing the residue by centrifugation is
The extract was used as a mycelium extract of Galix mushrooms. Production Example 2 Agaricus mushroom mycelium culture filtrate At the time of preparation of Production Example 1, the main culture solution of Agaricus mushroom mycelium was used.
After removing the mycelium, the culture solution is
A culture filtrate was used. [Production Example 3] Isomerized sugar mixture Dissolve 1 kg of D-glucose in 2,000 ml of purified water,
10% (w / v)% sodium hydroxide solution 1 while stirring
Add 0 ml and leave sealed at 21-23 ° C. That
Then, 10 (w / v)% hydroxyl so as to always maintain pH 9 or more.
Sodium chloride solution was added in 10 ml portions, for a total of 6
After adding 0 ml, add lactic acid to adjust the pH to 6.
Was completed, and this was designated as solution A. At this stage, L-glucose
Weight of glucose and glucose conversion products (mainly fructose)
The quantitative ratio becomes about 6: 4. On the other hand, lactose 1kg
Dissolved in 2,000 ml of purified water and stirred for 10 minutes.
10 ml of (w / v)% sodium hydroxide solution are added,
Leave tightly sealed at 21-23 ° C. Then, as in the case of solution A, p
10 (w / v)% sodium hydroxide so as to maintain H9 or more
10 ml each, and a total of 140 ml were added.
Thereafter, lactic acid was added to adjust the pH to 6, and the reaction was terminated.
And At this stage, isomerized lactose and lactose reduction
The weight ratio of the product (mainly galactose) was 5: 5.
You. Liquid A and liquid B are mixed at a weight ratio of 19: 1
To obtain an isomerized saccharide mixture. Table 1 shows the composition of this mixture.
Is shown. [0026] [Table 1] In the present invention, agaricus mushroom extract is different from
Fibroblasts obtained by combined use of
The synergistic activation of vesicles is described below. [Activity of dermal fibroblast metabolism activation] Human
2.0 × 10 cells per well^FourAnd
Seeded in a 96-well microplate for 24 hours
1.0 volume containing each of the production examples later shown in Table 2
37% in Dulbecco's minimum essential medium supplemented with 10% fetal calf serum
For 48 hours. Then 2- (4,5-dimethyl-2-thiazo
Ryl) -3,5-diphenyltetrazolium bromide (MT
T) was replaced with the above-mentioned medium containing 0.4 mg / ml.
Incubate at 7 ° C for 2 hours and open by opening the tetrazolium ring.
The formazan is extracted with 2-propanol and 550
Measured by absorbance in nm. In addition, 1.0 capacity
Cultured in Dulbecco's minimum essential medium supplemented with
Was used as a control, and Dulbet supplemented with 5.0% by volume of fetal calf serum.
A system cultured in the minimum essential medium was used as a positive control. Result is
Activation expressed as 100.0% absorbance in control
The results are shown in Table 2 by index. [0029] [Table 2] As a result, as shown in Table 2, Example 1 of operation
And in the operation example 2, the production example 1 and the production example 2 are used alone.
A small amount of Production Example 3 in comparison with Working Example 3 and Working Example 4
Approximately double fibroblast activation by using
Was observed, and the fibroblast activation was synergistically improved.
Was shown. Next, it was prepared using the above-mentioned production example.
The present invention will be described in more detail with reference to Examples. Examples 1-2 and Comparative Examples 1-4 O / W milk
Cosmetic serum Using each of the production examples shown in Table 3, O /
A W-emulsion type serum was prepared. (Prescription) (1) Squalane 5.0 (% by weight) (2) White petrolatum 2.0 (3) Beeswax 0.5 (4) Sorbitan sesquioleate 0.8 (5) Polyoxyethylene oleyl ether (20EO) 1.2 (6) Methyl paraoxybenzoate 0.1 (7) Propylene glycol 5.0 (8) Purified water Amount that total amount becomes 100 (9) 1.0% by weight aqueous solution of carboxyvinyl polymer 20.0 (10) Potassium hydroxide 0.1 (11) Ethanol 5.0 (12) Production example Amount shown in Table 3 (13) Fragrance 0.2 Production method: Mix oil phase components (1) to (5) and heat to 75 ° C
Dissolve and homogenize. On the other hand, the aqueous phase components of (6) to (8) are mixed,
Dissolve and heat to 75 ° C, add oil phase components and pre-emulsify
I do. After adding (9), homogenize uniformly with a homomixer
And add (10) to adjust the pH. After cooling at 40 ° C (1
1) to (13) are added, mixed and homogenized. [0033] [Table 3]Examples 1 and 2 and Comparative Examples 1 to 4
Prevention of wrinkles caused by ultraviolet rays using Comparative Example 4
The effect was evaluated. Wrinkle prevention effect, hairless mouse
Five animals were regarded as one group, and the examples and comparative examples were
Apply to the back once a day each, 1J / cm^Two/ Week long wavelength
Irradiate UVA for 50 weeks to hairless mice
Observe the occurrence of wrinkles in the test
Therefore, the score was calculated. At this time, apply only purified water
Group served as control. As a result, the average value of each group was calculated, and UV
Table 5 shows the relationship with A irradiation days. [0035] [Table 4] [0036] [Table 5] As shown in Table 5, isomerization of Production Example 3
In Comparative Example 3 application group containing only the sugar mixture, the control and
As in the case of the Comparative Example 5 application group in which no production example was blended, U
Minor wrinkles occur when VA irradiation days exceed 40 weeks
Was observed, and moderate wrinkles were observed after 50 weeks.
Had been. In contrast, Production Example 1 and Production Example 2
In Comparative Example 1 and Comparative Example 2
Even after 50 weeks, wrinkles were observed to the extent that slight wrinkles were observed.
Was significantly suppressed. On the other hand, Example 1
In Example 2, 1/5 of Comparative Example 1 and Comparative Example 2
Despite containing only Production Examples, Comparative Examples 1 and
And a more excellent wrinkle suppression effect than Comparative Example 2 was observed.
A small amount of Production Example 3 in which no effect on generation of cracks was observed
By doing so, suppression of wrinkle generation in Production Example 1 and Production Example 2
It was shown that the effect improved synergistically. Subsequently, Examples 1 and 2 of the present invention and
About Comparative Examples 1 to 4, anti-inflammatory action and wound healing
The promotion effect was evaluated. Artificial inflammation or wound formation
Examples and comparative examples were applied to each group using 5 mice per group.
Apply 0.5g each twice a day for 7 days, then flame on the 7th day
The condition of the affected site and the wound site was observed. Anti-inflammatory effect
"Effective", "slightly effective", "ineffective", promotion of wound healing
Regarding the progression effect, "complete healing", "almost healing", "healing"
Incomplete "
The results are shown in Table 6 below. [0039] [Table 6] As is clear from Table 6, the anti-inflammatory effect,
Regarding the effect of promoting wound healing,
None of the mice were evaluated as invalid in any case,
Effective anti-inflammatory action and wound healing in more than 4 mice
A healing promoting effect was observed, and Production Example 1 and Production Example 2
Comparative Example 1 in which Galix mushroom extract was blended alone in a 5-fold amount and
The same effect as that of Comparative Example 2 was obtained. Next, Examples 1 and 2 of the present invention and a comparison
For Example 1 to Comparative Example 4, an actual use test for 6 months was performed.
Was. As a panelist, noticeable wrinkles or elasticity
Females in their 40s and 60s who have skin symptoms such as reduction
And women in their 20s and 50s with remarkable skin roughness
Each group consisted of 20 people. Use test is performed for each group
Each of the example and the comparative example was used with a blind.
And observe the condition of the skin before and after the end of the use test.
I thought and went. About the improvement situation of wrinkles and skin elasticity
Has three stages: "improvement," "slight improvement," and "no change."
Table 7 shows the number of panelists who obtained each evaluation.
In addition, about the degree of wrinkles, please take photos and replicas
More, skin elasticity is measured with a cute meter
And evaluated. In addition, the roughness shown in Table 8
The skin condition was scored according to the criterion, and the average value of 20 people
Table 9 shows a comparison between before the start of the use test and after the end of the use test.
It was shown to. [0042] [Table 7] As shown in Table 7, the embodiment of the present invention was used.
Wrinkles and improvement of skin elasticity are not recognized in panel group
No improvement was observed in all panelists.
5 times the Agaricus mushroom extract of Production Examples 1 and 2
The improvement effect superior to Comparative Examples 1 and 2 containing
Admitted. On the other hand, only the isomerized saccharide mixture of Production Example 3 was contained.
In Comparative Example 3 having wrinkles and an improvement tendency of elasticity,
Panelists who acknowledged but did not change
There were 5 and 7 people. [0044] [Table 8] [0045] [Table 9] As shown in Table 9, the embodiment of the present invention is used.
In the treatment group, all panels showed a tendency to improve skin roughness.
Was used. On the other hand, in Production Examples 1 and 2,
Comparative Examples 1 and 5 containing 5 times the amount of Galix mushroom extract
In the case of No. 2, although there was a tendency to improve skin roughness,
Combination with the isomerized saccharide mixture of Preparation Example 4 results in rough skin
It was shown that the improvement effect was significantly improved. Example 1, Example 2 and Comparative Examples 1 to Comparative Examples
4 was used to conduct a moisture retention test. Moisturizing, temperature 20
In a constant temperature and humidity room with a relative humidity of 50%
0.01 ml / cm for the example^TwoSkin water 30 minutes after application
Use a high-frequency impedance meter (IBS, Sk
icon 200). In addition,
The skin of the part where the examples and comparative examples are not applied as a reference
The amount of water was measured, and the difference was defined as the increase in the amount of water in the epidermis. each
The average value of 10 panelists was calculated for each sample, and the results were calculated.
Are shown in Table 10. [0048] [Table 10] As a result, Examples 1 and 2 were uncoated.
48μS and 45μS epidermis moisture content from cloth part respectively
A comparison containing only Agaricus mushroom extract
About 2.5 times of Example 1 and Comparative Example 2, only the isomerized saccharide mixture
More than twice as high as Comparative Example 3 containing
Agaricus mushroom extract and isomerized sugar mixture
That the moisturizing effect is synergistically improved.
Was shown. The first and second embodiments of the present invention will be described.
In addition, during the above use test period, precipitation, separation,
No change in the state of the preparation such as aggregation, discoloration, or discoloration
Did not. In each of the working groups, the skin irritation
None of the panelists showed any reaction or skin sensitization reaction
Was. Next, the formulation of another embodiment of the present invention will be described. [0052] [Example 3] Lotion for skin (1) Ethanol 10.0 (wt%) (2) Hydroxyethyl cellulose 1.0 (3) Agaricus mycelium extract (Production Example 1) 0.2 (4) Isomerized sugar mixture (Production Example 3) 0.05 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 88.65 Production method: (1) to (6) are mixed and made uniform. [0053] Example 4 Emulsion for skin (1) Stearic acid 0.2 (% by weight) (2) Cetanol 1.5 (3) Vaseline 3.0 (4) Liquid paraffin 7.0 (5) Polyoxyethylene (10EO) monooleate 1.5 (6) Tocopherol acetate 0.5 (7) Glycerin 5.0 (8) Methyl paraoxybenzoate 0.1 (9) Triethanolamine 1.0 (10) Purified water 79.5 (11) Isomerized sugar mixture (Production Example 3) 0.2 (12) Agaricus mushroom mycelium culture filtrate (Production Example 2) 0.25 (13) Agaricus mushroom mycelium extract (Production Example 1) 0.25 Production method: Mix and heat the oil phase components (1) to (6)
Thaw and keep at 70 ° C. On the other hand, the aqueous phase components (7) to (10)
In this case, the mixture is heated to be uniform, and the temperature is set to 70 ° C. This aqueous phase component
The oil phase component is gradually added with stirring to emulsify and cool.
After cooling, add (11) to (13) at 40 ° C and mix. [0054] [Example 5] Gel for skin (1) Purified water 88.4 (% by weight) (2) Carboxyvinyl polymer 0.5 (3) Dipropylene glycol 10.0 (4) Methyl paraoxybenzoate 0.1 (5) Potassium hydroxide 0.1 (6) Agaricus mushroom mycelium culture filtrate (Production Example 2) 0.8 (7) Isomerized sugar mixture (Production Example 3) 0.1 Production method: After (2) is uniformly dissolved in (1), (4) is dissolved in (3).
Thaw and add, then add (5) to thicken, (6) and
(7) is added. [0055] Example 6 Skin Cream (1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl fatty acid ester 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20EO)         Sorbitan monolaurate 5.0 (8) Propylene glycol 5.0 (9) Methyl paraoxybenzoate 0.1 (10) Purified water 36.7 (11) Agaricus mushroom mycelium extract (Production Example 1) 0.5 (12) Isomerized sugar mixture (Production Example 3) 0.2 Production method: Mix and dissolve the oil phase components (1) to (7) to 75 ° C.
Heat. On the other hand, the aqueous phase components (8) to (10) were mixed and dissolved.
To 75 ° C. Next, the oil phase component is added to the aqueous phase component.
After pre-emulsification by adding
After cooling, (11) and (12) are added and mixed at 40 ° C. [0056] [Example 7] Oil-in-water emulsion ointment (1) White petrolatum 25.0 (% by weight) (2) Stearyl alcohol 25.0 (3) Glycerin 12.0 (4) Sodium lauryl sulfate 1.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 35.8 (7) Agaricus mushroom mycelium extract (Production Example 1) 1.0 (8) Isomerized sugar mixture (Production Example 3) 0.1 Production method: Mix and dissolve the oil phase components (1) to (4) to make them uniform
And heat to 75 ° C. On the other hand, dissolve (5) in (6)
Heat to 5 ° C, add the oil phase component to this and emulsify,
After cooling, add (7) and (8) at 40 ° C and mix. [0057] [Example 8] Lotion (1) Ethanol 10.0 (wt%) (2) 1,3-butylene glycol 5.0 (3) Agaricus mushroom mycelium culture filtrate (Production Example 2) 0.1 (4) Isomerized sugar mixture (Production Example 3) 0.05 (5) Fragrance 0.1 (6) Purified water 84.75 Production method: (1) to (5) are added sequentially to (6) and mixed and dissolved uniformly.
Understand. [0058] [Example 9] Emollient cream (water-in-oil type) (1) Liquid paraffin 30.0 (% by weight) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglyceryl oleate 5.0 (5) Sodium L-glutamate 1.6 (6) L-serine 0.4 (7) Propylene glycol 3.0 (8) Methyl paraoxybenzoate 0.1 (9) Purified water 52.55 (10) Fragrance 0.1 (11) Agaricus mushroom mycelium extract (Production Example 1) 0.1 (12) Agaricus mushroom mycelium culture filtrate (Production Example 2) 0.1 (13) Isomerized sugar mixture (Production Example 3) 0.05 Production method: (5) and (6) are dissolved in a part of (9) to 50 ° C.
Add slowly to (4) heated to 50 ° C. with stirring.
This was previously mixed and dissolved by heating at 70 ° C. (1)-
Disperse uniformly in (3), and add (7) and (8) to the rest of (9)
What was melted and heated to 70 ° C. was added with stirring,
Emulsify with a homomixer. After cooling, at 40 ° C (10) ~
(13) is added and mixed. [0059] [Example 10] Makeup base cream (1) Stearic acid 12.0 (% by weight) (2) Cetanol 2.0 (3) Glyceryl tri-2-ethylhexanoate 2.5 (4) Self-emulsifying glyceryl monostearate 2.0 (5) Propylene glycol 10.0 (6) Potassium hydroxide 0.3 (7) Purified water 69.0 (8) Titanium oxide 1.0 (9) Bengala 0.1 (10) Yellow iron oxide 0.4 (11) Fragrance 0.1 (12) Agaricus mushroom mycelium extract (Production Example 1) 0.5 (13) Isomerized sugar mixture (Production Example 3) 0.1 Production method: Mix the oil phase components (1) to (4) and heat to 75 ° C.
And make it uniform. On the other hand, the aqueous phase components (5) to (7)
Heat to 5 ° C and melt to make it uniform, then add the faces of (8) to (10)
And homogenously disperse with a homomixer. this
Add the oil phase component to the aqueous phase component, and mix with a homomixer.
After cooling, add (11)-(13) at 40 ° C and mix
You. [0060] [Example 11] Emulsion foundation (1) Stearic acid 2.0 (% by weight) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5) Decaglyceryl monoisopalmitate 9.0 (6) 1,3-butylene glycol 6.0 (7) Potassium hydroxide 0.1 (8) Methyl paraoxybenzoate 0.1 (9) Purified water 53.4 (10) Titanium oxide 9.0 (11) Talc 7.4 (12) Bengala 0.5 (13) Yellow iron oxide 1.1 (14) Black iron oxide 0.1 (15) Fragrance 0.1 (16) Agaricus mushroom mycelium culture filtrate (Production Example 2) 0.15 (17) Isomerized sugar mixture (Production Example 3) 0.05 Production method: Mix the oil phase components (1) to (5) and heat to 75 ° C.
And make it uniform. On the other hand, the aqueous phase components (6) to (9)
Heat to 5 ° C and dissolve to make it uniform, then add (10) to (14) face
And homogenously disperse with a homomixer. This water
Add the oil phase component to the phase component and homogenize with a homomixer
After emulsification, add (15)-(17) at 40 ° C and mix.
Combine. [0061] [Example 12] Hand cream (1) Cetanol 4.0 (% by weight) (2) Vaseline 2.0 (3) Liquid paraffin 10.0 (4) Glyceryl monostearate 1.5 (5) Polyoxyethylene (60EO)         Glyceryl isostearate 2.5 (6) Tocopherol acetate 0.5 (7) Glycerin 20.0 (8) Methyl paraoxybenzoate 0.1 (9) Purified water 59.1 (10) Agaricus mushroom mycelium extract (Production Example 1) 0.1 (11) Agaricus mushroom mycelium culture filtrate (Production Example 2) 0.1 (12) Isomerized sugar mixture (Production Example 3) 0.1 Production method: Mix and dissolve the oil phase components (1) to (6) to 75 ° C
Heat. On the other hand, the aqueous phase components (7) to (9) are mixed and dissolved.
To 75 ° C. Next, this aqueous phase component is added to the oil phase component.
After pre-emulsification by adding
And cooled, add (10)-(12) at 40 ° C and mix
You. [0062] As described in detail above, the agaricus mushroom mycelium
Combination of body extract and mycelium culture filtrate with isomerized sugar mixture
Dermal fibroblast activation and moisturizing
Use synergistically to reduce UV radiation on dermal fibroblasts
Has the effect of protecting against injury due to wrinkles, skin elasticity
Effective for preventing or improving skin aging symptoms such as reduction of skin
It also has an anti-inflammatory effect and a wound healing promoting effect.
The qualitative and safety were also good.

────────────────────────────────────────────────── ─── Continued on the front page (51) Int.Cl. ⁷ identification code FI A61K 35/84 A61K 35/84 A61P 17/16 A61P 17/16 (58) Fields surveyed (Int.Cl. ⁷ , DB name) A61K 7/00-7/50 JICST file (JOIS) CA (STN)

Claims (1)

(57) [Claims] [Claim 1] Agaricus blazei Murill
Mycelium extract and Agaricus blazei Muril
l) An external preparation for skin, comprising a mixture of one or two selected from mycelium culture filtrate and sugar isomerized by the action of an alkali hydroxide solution.