JP3053368B2 - Cosmetic and method for producing the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic and a method for producing the same, and more particularly, to a cosmetic which is excellent in antibacterial properties and ultraviolet absorption, prevents roughening and matting, and maintains beautiful skin, and a method for producing the same. About.

[0002]

2. Description of the Related Art Conventional cosmetics are formulated with various drugs for the purpose of various medicinal effects, but many of them have side effects even if a minimum amount of the medicinal effect is added. As a countermeasure, natural flora and fauna, especially plant extracts are blended (for example, loquat extract disclosed in Japanese Examined Patent Publication No. 5-17206), but those which are excellent in antibacterial properties, ultraviolet absorption properties, etc. Has not yet been found.

[0003]

SUMMARY OF THE INVENTION The present invention solves the above-mentioned problems of the prior art, and has excellent antibacterial properties and ultraviolet absorption, prevents roughening and matting, and maintains a beautiful skin. It is an object of the present invention to provide a manufacturing method thereof.

[0004]

Means for Solving the Problems In order to achieve the above object, the present inventors have conducted intensive studies on the characteristics of the components of the plant extract, and as a result, the saponin contained in the Yucca extract has been found to have antibacterial properties. It has excellent antibacterial properties, especially against yeast, and polyphenols, especially resveratrol (3,4 ', 5-trihydroxystilbene) contained in the Yucca extract, inhibit the viability of enzymes. In addition, taking advantage of the fact that saponin, flavone and resveratrol contained in the above-mentioned yucca extract are all excellent in ultraviolet ray absorbability, saponin, flavone and resveratrol are blended in cosmetics to make the skin rough and matte. The present inventors have found that a cosmetic that can prevent blemishes and maintain beautiful skin can be obtained, thereby completing the present invention.

According to the present invention, the present inventors have determined that a cosmetic containing a yucca extract is excellent in preventing rough skin and aging, and that the extract contains saponin, flavone and resveratrol. Was found and completed.

That is, the cosmetic of the first aspect of the present invention is
ucca) characterized by containing an extract. The above-mentioned “Yucca” includes Yucca Sidigera (Yucca)
schidigera), Atsubakimigayoran (Yuc
ca gloriosa Linn), Kimigayoran (Yucca recurvifolia Salis)
b), Itran (Yucca smalliana F
ernald), Senjuran (Yucca aloi)
folin Linn) can be used. One of these may be used, or two or more of these may be used. The above-mentioned "Yucca extract" may be an extract itself obtained by extracting from a raw Yucca with a predetermined extraction solvent, or a concentrated solution obtained by concentrating this extract,
Solids obtained by drying these (the shapes of powders, granules, etc. do not matter) may be used.

[0007] The yucca extract may contain saponin, flavone and resveratrol. The saponin, the flavone or the resveratrol may be a Yucca extract containing this component, or may be a fraction obtained from this extract. Further, these may be mixed, or the content of a plurality of components may be mixed.

The contents of the above-mentioned saponin, flavone and resveratrol are, as shown in the third invention, 10% of cosmetics.
When it is 0% by weight, it can be 0.001 to 5% by weight (preferably 0.01 to 1% by weight). The content of the material is not sufficiently exhibited the effect of the substance is less than 0.001 wt%, the content is more than 5 wt%, colored or because the defects such as fine odor appears. Also,
When the saponin, flavone and resveratrol are contained, the saponin is 10 parts by weight, the flavones are 3 to 5 parts by weight, and the resveratrol is 0.1 part by weight.
It is preferable to use 5 to 2 parts by weight.

The method for producing a cosmetic according to the fourth aspect of the present invention is a method for producing a powder after at least one of the roots and stems of Yucca is made into chips and dried, or dried into chips, and then crushed to produce powder. Thereafter, the powder is immersed and extracted in a mixed solvent of ethanol and water to obtain a Yucca extract, and then the Yucca extract is blended. The above “Yucca” is used in the same meaning as described above. And
As the Yucca raw material, usually, roots or stems can be used, but usually both are used. Etano-
The mixed solvent of water and water may have a mixing ratio of 75 to 95% by weight of ethanol as shown in the fifth invention. If the ethanol content is less than 75% by weight, the extraction component contains a large amount of sugar and the ratio of the saponin component becomes low, and the extraction of yucca takes too much time, resulting in an impossible rate.
When the content exceeds 95% by weight, the content of the saponin component decreases. As described above, the form of the “Yucca extract” may be an extract, a concentrate, or a solid.

The form of the cosmetic of the present invention and the cosmetic produced by the production method of the present invention are not particularly limited, and examples thereof include liquids (lotions, emulsions, etc.), creams and ointments. can do. If necessary, various additives commonly used in cosmetics, for example, aqueous components such as lower alcohols and polyhydric alcohols, powder components such as titanium oxide and borax, oils, and surfactants , A thickener, an antioxidant, a fragrance, a wrapping agent, a drug, and the like.

[0011]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. Preparation of Yucca extract, preparation of cosmetics and evaluation of its performance were performed by the following methods. (1) Preparation of Yucca Extract (Part 1) Yucca schidella
igera) was made into chips, dried in the sun, and ground to obtain a raw powder. 500 g of this bulk powder was immersed in five times the amount of 90% ethanol and left at room temperature for 24 hours.
Filtration gave a 90% ethanol extract. This 90% ethanol extract was heated at normal pressure, and ethanol was distilled off to obtain a concentrate of 30 to 40% by weight of the active ingredient. A 5-fold amount of distilled water was added to these concentrated liquids and left for 12 hours. Thereafter, the aqueous solution in the upper layer and the water-insoluble matter in the lower layer were separated by decantation, and filtered after the remaining amount became small. This aqueous solution is heated under reduced pressure to distill off water and concentrate.
At about 0%, distillation was stopped, and 35 g of a brown powder (referred to as "Yucca extract 1") was obtained by vacuum, freezing or spray drying. The main components of this powder are saponin (60-70% by weight in dry powder) and sugar (30 in dry powder).
４０40% by weight). The weight ratio between the saponin and the sugar is about 2: 1. Then, after filtering off the water-insoluble matter,
Vacuum drying was performed to obtain 15 g of a brown powder (referred to as “Yucca extract 1A”). The main components of this powder are flavone (75-85% by weight in dry powder) and resveratrol (15-25% by weight in dry powder). Incidentally, the weight ratio of this flavone to resveratrol is about 4: 1.

(Part 2) Active ingredient 30 obtained in Part 1
Using a column packed with an ion exchange resin (trade name "Diaion HP20", manufactured by Mitsubishi Chemical Corporation), 200 g of a concentrated solution of 40% to 40% is sequentially developed with water, 30% ethanol, and 80% ethanol. And fractionated. Water and 30% ethanol extract mainly contain sugar components, and 80% ethanol extract mainly contains saponin, flavone and resveratrol. Ethanol was distilled off from this 80% ethanol extract at normal pressure in the initial stage and at reduced pressure in the latter half.
A concentrate containing 40 to 50% of the active ingredient was obtained. Further, this concentrated liquid was dried under vacuum to obtain 35 g of a brown powder (referred to as "Yucca extract 2"). The main components of this powder are saponin (87-90% by weight in dry powder), flavone (10-8% by weight in dry powder) and resveratrol (3-2% by weight in dry powder). The weight ratio of saponin, flavone and resveratrol is 10: 4: 1.

(Part 3) Active ingredient 30 obtained in Part 1
Distilled water in an amount of 2 to 3 times was added to 100 g of the ４０40% concentrated solution, and the mixture was allowed to stand at room temperature overnight. Then, the aqueous solution was separated by decantation. This aqueous solution was subjected to initial water washing using the same column as above, and then extracted and fractionated with a 60% water mixed solution of acetone. The acetone solution was subjected to distillation under reduced pressure, concentrated and allowed to cool, then frozen and dried to obtain 27 g of a powder (referred to as “Yucca extract 3”). The main component of this powder is saponin (90 to 100% by weight in the dry powder).

(2) Preparation of Cosmetic The amount of the cosmetic (lotion) (composition) in Example 1 is expressed in parts by weight (hereinafter referred to as "parts"). 1) Glycerin 1.0 part 2) 1,3-butylene glycol 3.0 parts 3) Polyoxyethylene (15 mole addition) oleyl 0.3 parts alcohol ether 4) Yucca extract 1; (Saponin) : Sugar = 2: 1) 0.02 parts 5) Sodium citrate 0.1 parts 6) Ethanol 8.0 parts 7) Fragrance 0.03 parts 8) Purified water 87.55 parts Total 100.0 parts

(Preparation) Glycerin, 1,3-butylene glycol and sodium citrate are added to purified water and dissolved by stirring. Separately, Yucca extract 1, polyoxyethylene oleyl ether and perfume are dissolved in ethanol,
This was added to the above purified water solution, mixed uniformly, and filtered to obtain a lotion.

Cosmetic (Lotion) of Comparative Example 1 A lotion was obtained in the same composition and preparation as in Example 1 except that Yucca Extract 1 was not blended.

[0017] Cosmetic of Example 2 (Emulsion) (Composition) 1) Refined jojoba oil 2.0 parts 2) Stearic acid 1.5 parts 3) Cetyl alcohol 0.3 parts 4) Polyoxyethylene (8 mol addition) ) Monooleic acid 1.0 part Ester 5) Sorbitan monostearate 6.5 parts 6) Yucca extract 2; 0.05 part (Saponin: flavone: resveratrol = 10: 4: 1) 7) Propylene Glycol 3.0 parts 8) Ethanol 3.0 parts 9) Fragrance 0.01 parts 10) Purified water 82.64 parts Total 100.0 parts

(Preparation) Yucca extract 2 and a fragrance are dissolved in ethanol to obtain an ethanol solution. A purified aqueous solution obtained by adding propylene glycol to purified water is 70-80.
Keep at ° C. The other components are mixed together and dissolved by heating.The resulting solution is kept at 70 to 80 ° C., and added to the purified aqueous solution while stirring to emulsify the mixture. After the mixture becomes homogeneous, the mixture is further stirred with a homomixer. Finish by adding the above ethanol solution. Further, this liquid was cooled to 30 ° C. by a heat exchanger (an on-liner having a mixing and kneading function) to obtain an emulsion.

Cosmetic (cream) of Example 3 (Composition) 1) Refined jojoba oil 10.0 parts 2) Squalane 35.0 parts 3) Stearyl alcohol 3.0 parts 4) Polyoxyethylene (15) Mole addition) palmitic acid 3.0 parts ester 5) sorbitan monostearate 1.0 part 6) yucca extract 1; (saponin: sugar = 2: 1) 0.1 part 7) propylene glycol 8. 0 parts 8) Perfume 0.01 parts 9) Purified water 39.89 parts Total 100.0 parts

(Preparation) The raw materials of 1) to 5) are heated at 70 to 80 ° C.
The raw material 6) heated to 75 ° C.
7. Add to the aqueous solutions of 7) and 9 with stirring and pre-emulsify. Next, the raw material 8) was added, and the mixture was stirred with a homomixer to make the emulsified particles fine and uniform, and then rapidly cooled to 30 ° C. to obtain a cream.

Cosmetic (Ointment) of Example 4 (Composition) 1) Yucca extract 3 0.5 part 2) Stearyl alcohol 15.0 parts 3) Beeswax 20.0 parts 4) Polyoxyethylene (5) Molar addition) sorbitan mono 0.3 part stearate 5) Polyoxyethylene (15 mole addition) monooleic acid 0.2 part ester 6) Vaseline 35.0 parts 7) Glycerin 5.0 parts 8) Purified water 24. 0 parts Total 100.0 parts

(Preparation) Raw materials 1) to 7) were heated and dissolved at 75 ° C., added to purified water kept at 70 ° C., emulsified by a homomixer, and then cooled to obtain an ointment.

(3) Evaluation of Skin Roughness Improvement Performance The following performance tests were conducted to clarify the performance of the above cosmetics. Using the lotion obtained in Example 1 and the lotion obtained in Comparative Example 1, a skin roughness improvement effect test was performed on a human body panel. That is, a replica of the skin surface morphology of the face of a healthy female was taken using a replica method using a Myrison resin and observed with a microscope (17 ×). Each of the lotions was applied once a day to the left and right half of the face using 25 people (rough panel) whose skin roughness was judged to be 1 or 2 according to the criteria shown in Table 1 based on the state of the skin pattern and the peeled state of the cornea. It was applied for two weeks. 2
After one week, the skin surface morphology of the face was observed again by the replica method described above, and evaluated according to the criteria shown in Table 1. Table 2 shows the results.

[0024]

[Table 1]

[0025]

[Table 2]

The following can be seen from Table 2. In other words, in the case of the face using the lotion containing Yucca extract 1 (Example 1), no “skins, disappearance of skin ridges, and widespread turning of the stratum corneum” were 0, and “skins, skin hills” Were unclear, the horny layer was turned up ", 11 were" skins and ridges were sharp, "and 12 were" skins and ridges were sharp and tidy. " In the case of the face using the lotion not containing the extract 1 (Comparative Example 1), 10 persons “skin folds, disappearance of skin ridges, turning over of a wide range of stratum corneum”, “skin ridges and skin hills are unclear, There were 8 people who turned up the stratum corneum, 0 people who had clear skin and ridges, and 0 people who had clear skin and ridges. Therefore, the lotion containing the Yucca extract 1 has a remarkable effect of preventing skin roughness and matting and maintaining beautiful skin, as compared with the lotion not containing the Yucca extract 1.

(4) Evaluation of ultraviolet absorption performance The ultraviolet absorption performance of saponin, flavone and resveratrol is shown in FIG. 1 (saponin), FIG. 2 (flavone and resveratrol) and FIG. 3 (resveratrol). From these results, it can be seen that all the substances show excellent ultraviolet absorption performance. In particular, saponin is 260-340
In nm, flavone shows excellent ultraviolet absorption performance at about 260-320 nm, and resveratrol shows excellent ultraviolet absorption performance at 280-340 nm. Therefore, it can be seen that, if it contains saponin and / or flavone and resveratrol, it exhibits ultraviolet absorption performance in a wide range. The resveratrol used in this test was an organic synthetic product (purity 9
8% by weight).

(5) Evaluation of Whitening Effect of Resveratrol In order to observe the whitening effect of resveratrol, the effect of resveratrol on B16 melanoma cells having melanin-producing ability was examined. The resveratrol used in this test was an organic synthetic product (purity: 98% by weight). (Test method) 1) Cell culture Transfer the B16 melanoma cell solution to a culture flask 25 cm ² , add 4 ml of a culture solution (DMEM containing 10% FBS), and condition at 37 ° C., 5% -CO ₂ /95% -air. Cultured underneath. When the cells became semi-confluent, the cells were collected by trypsin treatment and 5 × 10 ⁴ cells / 1
The seeds were inoculated to a 96-well multiplet so as to have a volume of 00 μl, and cultured again at 37 ° C. for 24 hours under the conditions of 5% -CO ₂ /95% -air.

2) Measurement of tyrosinase biosynthesis inhibitory activity After culturing in a 96-well multiple plate for 24 hours, the culture solution was removed, 195 μl of a new culture solution and 5 μl of a sample solution of each concentration were added, and the mixture was added at 37 ° C., 5% -CO _2/95% -ai
Culture was performed for 24 hours under the conditions of r. After 24 hours, the culture solution containing the sample was removed, washed with PBS, and washed with 1% -Triton X-
Add 50 μl of 100 / PBS solution, stir for 30 minutes,
Add 100 μl of a 10 mM-DOPA / PBS solution, add 3
After incubating for 3 hours at 7 ° C. and 5% -CO ₂ /95% -air, the absorbance was measured at a wavelength of 475 nm. Tyrosinase biosynthesis inhibition rate (%) = [(AB) / A]
× 100 A; Absorbance without sample added B: Absorbance when sample was added

3) Measurement of cell viability After culturing in a 96-well multiple plate for 24 hours, the culture solution was removed, 195 μl of a new culture solution and 5 μl of a sample solution of each concentration were added, and the mixture was added at 37 ° C., 5% -CO ₂ / 95% -ai
Culture was performed for 24 hours under the conditions of r. After 24 hours, the culture solution containing the sample was removed, washed with PBS, and the MTT-containing medium 10 was removed.
0 μl was added, and the mixture was incubated for 4 hours. Four hours later,
The culture solution was removed, 200 μl of isopropyl alcohol was added, and the mixture was allowed to stand for 30 minutes, and the absorbance was measured at wavelengths of 570 nm and 655 nm. Cell viability = [Y / X] × 100 X; value of absorbance without sample (570 nm-655 nm) Y; absorbance with sample added (570 nm-655)
(nm) value The sample solution used was a 50% aqueous ethanol solution.

(Test Results) The results of these tests are shown in Table 3. The following can be seen from Table 3. That is, resveratro-
At a concentration of 10 ppm, a tyrosinase biosynthesis inhibition rate of 51.4% was observed, and the cell viability was 10%.
No cytotoxicity was observed at 0.3%. That is 5
Even at ppm, no cytotoxicity was observed at all, and a relatively excellent inhibition rate of 22.1% was shown. On the other hand, when it was 50 ppm or more, it exhibited its excellent inhibition rate and exhibited a certain degree of cytotoxicity with a cell viability of 32.3%. When it is 100 ppm or more, the cell viability is as low as 8.4%, and therefore cytotoxicity is strongly observed. When it is 1 ppm or less, the inhibition rate is as low as 1.2% or less, and therefore, excellent whitening. No effect was shown. From the above, the concentration is 5 to 50 ppm
In the case of, cytotoxicity was suppressed to a certain extent or more, and an excellent whitening effect was exhibited to a certain extent or more. Among them, in particular, at 5 to 10 ppm, no cytotoxicity was observed at all, and some excellent whitening effect was exhibited. Further, from the above results, the concentration was 3 to 4
According to 0 ppm, the tyrosinase biosynthesis inhibitory effect and the cytotoxicity-reducing ability can be excellently balanced.

[0032]

[Table 3]

(6) Comprehensive Evaluation The effects of adding saponin, flavone, and resveratrol contained in the yucca extract to cosmetics are summarized. Cosmetics containing the above-mentioned saponin almost solely have an effect of improving skin roughness and also have an ultraviolet absorbing effect. That is, saponin is effective for use in cosmetics. The above-mentioned resveratrol alone has a remarkable whitening effect at a low concentration, but it is very difficult to adjust the concentration. Therefore, it is better to use resveratrol in combination with saponin and flavone than in use alone. Useful as cosmetics. Flavone has an ultraviolet absorbing effect. Therefore, it is considered that flavone is also effective for use in cosmetics.

(7) Extraction conditions When the bulk powder obtained from Yucca is extracted with 80% ethanol,
Since saponin is dissolved in 80% ethanol, a high-concentration extract of saponin can be obtained. Therefore, when saponin is contained as a main component, this can be applied. From these results, similar results can be obtained with 100% ethanol. By immersing the bulk powder in 90% ethanol, an extract containing three components of saponin, flavone and resveratrol can be obtained. Therefore, this can be applied when a cosmetic containing the above three components is to be prepared. With water alone and 30% ethanol, the above three components are not extracted.
The above ethanol is considered necessary. Therefore, the above 3
To obtain an extract containing the components, 40-90% ethanol, preferably 50-90% ethanol, more preferably 70-90% ethanol can be applied.

The present invention is not limited to the specific embodiments described above, but can be variously modified within the scope of the present invention according to the purpose and application. That is, one of the root and the stem may be used as the extraction raw material.
Further, in the above embodiment, the extraction is performed using the pulverized material obtained from the chip, but the present invention is not limited to this, and the chip may be extracted as it is, or the roots and / or stems may be directly pulverized as the pulverized material. This crushed powder may be used, and the size of the crushed powder is not particularly limited. The extraction conditions, in particular, the type of the extraction solvent, the extraction time, the temperature thereof, and the like can be variously changed. The mixing ratio of saponin, flavone, and resveratrol obtained by fractionation (fractionation) from the Yucca extract may be appropriately changed according to the purpose. Furthermore, saponin obtained by fractionation (fractionation) from this yucca extract,
One or two of flavone and resveratrol may be appropriately blended, or may be subjected to extraction conditions without fractionation.
An extract (whether liquid or dry solid) which is obtained by extracting one or two of these may be used.

[0036]

EFFECTS OF THE INVENTION The cosmetics of the present invention and the cosmetics obtained by the method for producing the same are saponin, flavone and resveratro.
Since it contains phenol, it has excellent antibacterial properties and ultraviolet absorption properties, has the effect of preventing rough and matte skin and maintaining beautiful skin.

[Brief description of the drawings]

FIG. 1 is a graph showing the UV absorption performance of Yucca extract 3 obtained in an example.

FIG. 2 is a graph showing the ultraviolet absorption performance of Yucca extract 1A obtained in Example.

FIG. 3 is a graph showing the ultraviolet absorption performance of resveratrol.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI A61K 35/78 A61K 35/78 C09K 3/00 104 C09K 3/00 104Z // A61P 17/00 A61P 17/00 31/04 31 / 04 43/00 111 43/00 111 C12N 9/99 C12N 9/99 (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 7/00 A61K 7/42 A61K 7/48 A61K 35/78 C09K 3/00 104

Claims (5)

(57) [Claims] 1. A cosmetic comprising a Yucca extract. 2. The cosmetic according to claim 1, wherein said yucca extract contains saponin, flavone and resveratrol. 3. The cosmetic according to claim 2, wherein the content of said saponin, flavone and resveratrol is 0.001 to 5% by weight, based on 100% by weight of said cosmetic. 4. A method in which at least one of the roots and stems of Yucca (Yucca) is chipped and dried, or dried and chipped, and then crushed to produce a powder, and then the powder is mixed with ethanol. A method for producing cosmetics, comprising immersing and extracting in a mixed solvent of water to obtain a Yucca extract, and then blending the Yucca extract. 5. A mixed solvent of ethanol and water in a mixing ratio of 75 to 95% by weight of ethanol.
The method for producing the cosmetic according to the above.