JP2660837B2 - Test strip for detecting peroxide active substances - Google Patents
Test strip for detecting peroxide active substancesInfo
- Publication number
- JP2660837B2 JP2660837B2 JP62238176A JP23817687A JP2660837B2 JP 2660837 B2 JP2660837 B2 JP 2660837B2 JP 62238176 A JP62238176 A JP 62238176A JP 23817687 A JP23817687 A JP 23817687A JP 2660837 B2 JP2660837 B2 JP 2660837B2
- Authority
- JP
- Japan
- Prior art keywords
- detecting
- peroxide active
- active substance
- test strip
- test piece
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000012360 testing method Methods 0.000 title claims description 37
- 239000013543 active substance Substances 0.000 title claims description 26
- 150000002978 peroxides Chemical class 0.000 title claims description 24
- 230000001590 oxidative effect Effects 0.000 claims description 19
- 150000002432 hydroperoxides Chemical class 0.000 claims description 16
- 239000006097 ultraviolet radiation absorber Substances 0.000 claims description 9
- 239000008280 blood Substances 0.000 claims description 7
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000006096 absorbing agent Substances 0.000 claims description 6
- DBOSBRHMHBENLP-UHFFFAOYSA-N 4-tert-Butylphenyl Salicylate Chemical group C1=CC(C(C)(C)C)=CC=C1OC(=O)C1=CC=CC=C1O DBOSBRHMHBENLP-UHFFFAOYSA-N 0.000 claims description 3
- QUAMTGJKVDWJEQ-UHFFFAOYSA-N octabenzone Chemical compound OC1=CC(OCCCCCCCC)=CC=C1C(=O)C1=CC=CC=C1 QUAMTGJKVDWJEQ-UHFFFAOYSA-N 0.000 claims description 3
- 210000002700 urine Anatomy 0.000 claims description 2
- 239000013076 target substance Substances 0.000 claims 1
- 150000003852 triazoles Chemical class 0.000 claims 1
- 238000000034 method Methods 0.000 description 16
- 239000000243 solution Substances 0.000 description 11
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 239000002250 absorbent Substances 0.000 description 6
- 230000002745 absorbent Effects 0.000 description 6
- -1 polyethylene Polymers 0.000 description 6
- 210000001124 body fluid Anatomy 0.000 description 5
- 239000010839 body fluid Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000005470 impregnation Methods 0.000 description 5
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 5
- 229960004889 salicylic acid Drugs 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 229920003023 plastic Polymers 0.000 description 4
- 239000000080 wetting agent Substances 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- RWGFKTVRMDUZSP-UHFFFAOYSA-N cumene Chemical compound CC(C)C1=CC=CC=C1 RWGFKTVRMDUZSP-UHFFFAOYSA-N 0.000 description 2
- 238000002845 discoloration Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- ZQBAKBUEJOMQEX-UHFFFAOYSA-N phenyl salicylate Chemical compound OC1=CC=CC=C1C(=O)OC1=CC=CC=C1 ZQBAKBUEJOMQEX-UHFFFAOYSA-N 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- OLNZIANIUDPLRC-UHFFFAOYSA-N 1,3-benzothiazol-2-amine;sulfuric acid Chemical compound OS(O)(=O)=O.C1=CC=C2SC(N)=NC2=C1 OLNZIANIUDPLRC-UHFFFAOYSA-N 0.000 description 1
- MEZZCSHVIGVWFI-UHFFFAOYSA-N 2,2'-Dihydroxy-4-methoxybenzophenone Chemical compound OC1=CC(OC)=CC=C1C(=O)C1=CC=CC=C1O MEZZCSHVIGVWFI-UHFFFAOYSA-N 0.000 description 1
- ZXDDPOHVAMWLBH-UHFFFAOYSA-N 2,4-Dihydroxybenzophenone Chemical compound OC1=CC(O)=CC=C1C(=O)C1=CC=CC=C1 ZXDDPOHVAMWLBH-UHFFFAOYSA-N 0.000 description 1
- JGBAASVQPMTVHO-UHFFFAOYSA-N 2,5-dihydroperoxy-2,5-dimethylhexane Chemical compound OOC(C)(C)CCC(C)(C)OO JGBAASVQPMTVHO-UHFFFAOYSA-N 0.000 description 1
- LHPPDQUVECZQSW-UHFFFAOYSA-N 2-(benzotriazol-2-yl)-4,6-ditert-butylphenol Chemical compound CC(C)(C)C1=CC(C(C)(C)C)=CC(N2N=C3C=CC=CC3=N2)=C1O LHPPDQUVECZQSW-UHFFFAOYSA-N 0.000 description 1
- OFDNTJWAQFCUEM-UHFFFAOYSA-N 2-(benzotriazol-2-yl)-4-tert-butylphenol;2-(benzotriazol-2-yl)-4-methylphenol Chemical compound CC1=CC=C(O)C(N2N=C3C=CC=CC3=N2)=C1.CC(C)(C)C1=CC=C(O)C(N2N=C3C=CC=CC3=N2)=C1 OFDNTJWAQFCUEM-UHFFFAOYSA-N 0.000 description 1
- HJVAFZMYQQSPHF-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanol;boric acid Chemical compound OB(O)O.OCCN(CCO)CCO HJVAFZMYQQSPHF-UHFFFAOYSA-N 0.000 description 1
- UHGULLIUJBCTEF-UHFFFAOYSA-N 2-aminobenzothiazole Chemical class C1=CC=C2SC(N)=NC2=C1 UHGULLIUJBCTEF-UHFFFAOYSA-N 0.000 description 1
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- JRBJSXQPQWSCCF-UHFFFAOYSA-N 3,3'-Dimethoxybenzidine Chemical compound C1=C(N)C(OC)=CC(C=2C=C(OC)C(N)=CC=2)=C1 JRBJSXQPQWSCCF-UHFFFAOYSA-N 0.000 description 1
- NUIURNJTPRWVAP-UHFFFAOYSA-N 3,3'-Dimethylbenzidine Chemical compound C1=C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 NUIURNJTPRWVAP-UHFFFAOYSA-N 0.000 description 1
- FRIBMENBGGCKPD-UHFFFAOYSA-N 3-(2,3-dimethoxyphenyl)prop-2-enal Chemical compound COC1=CC=CC(C=CC=O)=C1OC FRIBMENBGGCKPD-UHFFFAOYSA-N 0.000 description 1
- SNCJAJRILVFXAE-UHFFFAOYSA-N 9h-fluorene-2,7-diamine Chemical compound NC1=CC=C2C3=CC=C(N)C=C3CC2=C1 SNCJAJRILVFXAE-UHFFFAOYSA-N 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 229920001651 Cyanoacrylate Polymers 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 239000001116 FEMA 4028 Substances 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- 229940027991 antiseptic and disinfectant quinoline derivative Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 1
- WZJYKHNJTSNBHV-UHFFFAOYSA-N benzo[h]quinoline Chemical class C1=CN=C2C3=CC=CC=C3C=CC2=C1 WZJYKHNJTSNBHV-UHFFFAOYSA-N 0.000 description 1
- RWCCWEUUXYIKHB-UHFFFAOYSA-N benzophenone Chemical compound C=1C=CC=CC=1C(=O)C1=CC=CC=C1 RWCCWEUUXYIKHB-UHFFFAOYSA-N 0.000 description 1
- 239000012965 benzophenone Substances 0.000 description 1
- QRUDEWIWKLJBPS-UHFFFAOYSA-N benzotriazole Chemical compound C1=CC=C2N[N][N]C2=C1 QRUDEWIWKLJBPS-UHFFFAOYSA-N 0.000 description 1
- 239000012964 benzotriazole Substances 0.000 description 1
- 229960004853 betadex Drugs 0.000 description 1
- OCWYEMOEOGEQAN-UHFFFAOYSA-N bumetrizole Chemical compound CC(C)(C)C1=CC(C)=CC(N2N=C3C=C(Cl)C=CC3=N2)=C1O OCWYEMOEOGEQAN-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 201000003146 cystitis Diseases 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 235000019329 dioctyl sodium sulphosuccinate Nutrition 0.000 description 1
- YHAIUSTWZPMYGG-UHFFFAOYSA-L disodium;2,2-dioctyl-3-sulfobutanedioate Chemical compound [Na+].[Na+].CCCCCCCCC(C([O-])=O)(C(C([O-])=O)S(O)(=O)=O)CCCCCCCC YHAIUSTWZPMYGG-UHFFFAOYSA-L 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 210000003736 gastrointestinal content Anatomy 0.000 description 1
- 238000012812 general test Methods 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 230000002008 hemorrhagic effect Effects 0.000 description 1
- 201000008383 nephritis Diseases 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- DXGLGDHPHMLXJC-UHFFFAOYSA-N oxybenzone Chemical compound OC1=CC(OC)=CC=C1C(=O)C1=CC=CC=C1 DXGLGDHPHMLXJC-UHFFFAOYSA-N 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 229960000969 phenyl salicylate Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- PTMHPRAIXMAOOB-UHFFFAOYSA-N phosphoramidic acid Chemical compound NP(O)(O)=O PTMHPRAIXMAOOB-UHFFFAOYSA-N 0.000 description 1
- XNGIFLGASWRNHJ-UHFFFAOYSA-L phthalate(2-) Chemical compound [O-]C(=O)C1=CC=CC=C1C([O-])=O XNGIFLGASWRNHJ-UHFFFAOYSA-L 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 150000003248 quinolines Chemical class 0.000 description 1
- 150000005839 radical cations Chemical class 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
- 210000004916 vomit Anatomy 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は体液や排泄物等の試料中の過酸化活性物質を
検出するための試験片に関するものである。
〔従来の技術〕
体液や排泄物、例えば、尿，吐物，胃腸内容物，糞便
等に含まれている過酸化活性物質、特に血液の検出は、
例えば、腎炎，腫瘍，出血性素因，膀胱炎等の疾病を早
急に発見する方法の１つとして使用されているが、通
常、前述疾病に起因する試料中の血液の含有量は極めて
少なく、肉眼での鑑別が殆んど困難である。
このため、試料中の血液の検出には、試料を顕微鏡観
察する方法が採られてきたが、この方法は再現性に乏し
いばかりでなく、操作が複雑であることから、多数の検
体を迅速に処理することができないという欠点を有して
いる。
前記顕微鏡観察による方法の欠点を解消する方法とし
て、有機ヒドロペルオキシドと酸化呈色指示薬とが担持
されている担体からなる試験片を使用する方法、すなわ
ち、体液や排泄物等の試料中に含有されている過酸化活
性物質のペルオキシダーゼ様活性によってヒドロペルオ
キシドを分解し、その結果発生する酸素が前記試験片中
の酸化呈色指示薬を呈色させる原理を使用する方法が採
られている。
然して、前記過酸化活性物質検出用の試験片において
は、該試験片の感度を高めたり、その安定化を意図した
り等の諸種の試験片が提案されている。例えば試験片中
に存在している有機ヒドロペルオキシドと酸化呈色指示
薬との間に介入する物質を安定剤として添加するものと
して、特開昭49−54093号公報にはリン酸アミドを、ま
た特開昭53−115288号公報にはトリエタノールアミンボ
レート等のホウ酸エステルを介入させ、有機ヒドロペル
オキシドと酸化呈色指示薬との間の安定性の向上を計る
ことが記載されている。更には、特開昭56−147066号公
報には、
等の安定剤を添加することによって過酸化活性物質の不
在下において有機ヒドロペルオキシドと酸化呈色指示薬
とが反応してしまうのを防ぎ、経時安定性の向上を計る
ことが、また試験片中に存在する有機ヒドロペルオキシ
ドと酸化呈色指示薬とを完全に隔離させることによっ
て、有機ヒドロペルオキシドと酸化呈色指示薬との間の
安定性の向上を計る方法として、例えば特開昭39−1474
7号公報にはマイクロカプセル化を利用する方法が、ま
た特開昭51−65694号公報にはフィルム形成性重合体を
利用する方法がそれぞれ開示されている。
〔発明が解決しようとする問題点〕
ところで、前記従来の過酸化活性物質検出用の試験片
においては、該試験片中に存在している有機ヒドロペル
オキシドと酸化呈色指示薬との間に発生する酸化還元反
応による安定性の低下は効果的に阻止されているもの
の、大気中の酸化物質（光特に紫外線）の作用による変
色や感度の低下までをも阻止し得るものではなく、これ
が品質のばらつきの要因となっているばかりでなく、長
期保存性能にも欠ける等の欠点を有している。
これに対して本発明の過酸化活性物質検出用の試験片
は、大気中の酸化物質（光特に紫外線）の作用による変
色，感度低下が無く、安定した品質で、しかも長期保存
性能に優れた作用を奏するものである。
〔問題点を解決するための手段〕
本発明の過酸化活性物質検出用の試験片は、担体に、
少なくとも、有機ヒドロペルオキシド，酸化呈色指示
薬、及び紫外線吸収剤が担持されているものであり、前
記有機ヒドロペルオキシド，酸化呈色指示薬、及び紫外
線吸収剤のほかに、この種の試験片における一般的な担
持物質、例えば、緩衝剤，湿潤剤，増感剤等が所望に応
じて担持されているものである。
前記構成からなる本発明の過酸化活性物質検出用の試
験片は、一般的には、濾紙，綿，不織布，ガラス繊維等
の吸収性担体に、前記有機ヒドロペルオキシド，酸化呈
色指示薬，及び紫外線吸収剤、更にはその他の物質を担
持させ、これを、例えば、ポリエチレン，ポリプロピレ
ン，ポリスチレン等のプラスチック片からなる基体に貼
着した構成からなるものが最も一般的な形態である。
吸収性担体に担持される有機ヒドロペルオキシドは液
状でも固体状でも良いが、一般にヒドロペルオキシドは
光や熱に対して不安定であるため、より安定な有機ヒド
ロペルオキシドを選択することが好ましく、例えば、ｔ
−ブチルヒドロペルオキシド，パラメタンヒドロペルオ
キシド，クメンヒドロペルオキシド,2,5−ジメチルヘキ
サン−2,5−ジヒドロペルオキシド等の１種または２種
以上の混合物が使用される。
酸化呈色指示薬は、酸化作用を受けて発色するもので
あれば良く、例えば、オルトトリジン、3,3′,5,5′−
テトラメチルベンチジン、ベンチジン、パラジアニシジ
ン、オルトジアニシジン、2,7−ジアミノフルオレン等
が使用される。
更に、紫外線吸収剤として使用される主なものには、
サリチル酸系紫外線吸収剤
フェニルサリシレート、
Ｐ−tert−ブチルフェニルサリシレート、
Ｐ−オクチルフェニルサリシレート、
ベンゾフェノン系紫外線吸収剤
2,4−ジヒドロキシベンゾフェノン、
ヒドロキシ−４−メトキシベンゾフェノン、
２−ヒドロキシ−４−オクトキシベンゾフェノン、
2,2′−ジヒドロキシ−４−メトキシベンゾフェノン、
ベンゾトリアゾール系紫外線吸収剤
２−（２′−ヒドロキシ−５′−メチルフェニル）ベン
ゾトリアゾール、
２−（２′−ヒドロキシ−５′−tert−ブチルフェニ
ル）ベンゾトリアゾール、
２−（２′−ヒドロキシ−３′,5′−ジ−tert−ブチル
フェニル）ベンゾトリアゾール、
２−（２′−ヒドロキシ−３′−tert−ブチル−５′−
メチルフェニル）−５−クロロベンゾトリアゾール、
シアノアクリレート系紫外線吸収剤
２−エチルヘキシル−２−シアノ−3,3′−ジフェニル
アクリレート、
エチル−２−シアノ−3,3′−フェニルアクリレート、
等が存する。
尚、担持物質を吸収性担体に担持させる際に利用され
る含浸液の調製は、有機ヒドロペルオキシド１〜５重量
％、酸化呈色指示薬0.1〜1.0重量％、紫外線吸収剤0.1
〜0.5重量％程度の濃度に調製されるものである。
更に、本発明の過酸化活性物質検出用の試験片に所望
に応じて担持されている一般的な担持物質たる緩衝剤
は、本発明の試験片による過酸化物質の検出作用を鋭敏
に行ない得るようにするためのものであり、担持物質を
含有している含浸液のpHが４〜７、特に５〜６に保持さ
れ得るように、クエン酸塩，燐酸塩，コハク酸塩，フタ
ル酸塩等の緩衝液が使用される。
また、湿潤剤は、例えば、試験片中に検体を浸透させ
易くしたり、酸化呈色指示薬が酸化されて生じたラジカ
ルカチオンを安定化させたりするためのもので、これら
の両作用を兼備する湿潤剤としては、例えば、ラウリル
硫酸ナトリウム，ドデシルベンゼンスルホン酸ナトリウ
ム，ジオクチルスルホコハク酸ナトリウム等の陰イオン
性界面活性剤が使用される。
更に、増感剤は、体液及び排泄物中の過酸化活性物質
特に血液の検出が、臨床医学上、100万倍希釈の血液で
も検出し得ることが要求されることの関係から使用され
るもので、例えば、キノリン誘導体，ベンゾキノリン誘
導体，ピリジン誘導体,2−アミノベンゾチアゾール誘導
体等が、含浸液中にて0.05〜1.0重量％で使用される。
吸収性担体に担持物質を担持させる最も一般的な方法
は、水−アルコール混合液中に所定量の有機ヒドロペル
オキシド，緩衝剤，増感剤，湿潤剤を溶解させた溶液に
濾紙を浸漬し、これを60〜80℃で乾燥した後、更に、所
定量の酸化呈色指示薬と紫外線吸収剤とが溶解されてい
る含浸液を含浸させ、これを乾燥させる方法であり、担
持物質が担持されている吸収性担体例えば濾紙小片を、
所定のプラスチック片等に貼着することによって、本発
明の過酸化活性物質検出用の試験片を容易に得ることが
できるものである。
〔実 施 例〕
以下、本発明の過酸化活性物質検出用の試験片の具体
的な構成を実施例に基いて説明する。
実施例１
クロマトグラフィー用の濾紙〔ワットマン（社）製:3
MM〕に下記第１含浸溶液を含浸させてから60℃にて60分
間乾燥し、次いで、同じく下記第２含浸液で含浸し、50
℃にて20分間乾燥した後プラスチック板に両面テープを
貼着し、これを短冊状に裁断して、幅5mm、長さ80mmの
プラスチック片からなる基体の片隅みに、５×5mmの濾
紙が貼着されている本発明の１実施例品たる過酸化活性
物質検出用の試験片〔１〕を得た。
第１含浸溶液
（１） １モルクエン酸塩緩衝液 ……50ml
（pH5.25）
（２） クメンヒドロペルオキシド
−β−シクロデキストリン包接体 ……12.5g
（３） ラウリル硫酸ナトリウム ……2.0g
（４） ２−アミノベンゾチアゾール硫酸塩 ……0.3g
（５） エタノール ……30ml
を蒸留水にて100mlに調製したもの。
第２含浸溶液
（１） オルトトリジン ……0.5g
（２） ポリビニルピロリドン ……1.5g
（Ｋ−90）
（３） サリチル酸 ｐ−tert−ブチルフェニルエステ
ル ……1.0g
をトルエンにて100mlに調製したもの。
実施例２
前記実施例１における第２含浸溶液のサリチル酸 ｐ
−tert−ブチルフェニルエステルの代わりに、２−ヒド
ロキシ−４−オクトキシベンゾフェノン1.0gを使用する
以外は全て前記実施例１と同一の手順を施し、本発明の
１実施例品たる過酸化活性物質検出用の試験片〔２〕を
得た。
実施例３
前記実施例１における第２含浸溶液のサリチル酸 ｐ
−tert−ブチルフェニルエステルの代わりに、２−
（２′−ヒドロキシ−３′−tert−ブチル−５′−メチ
ルフェニル）−５−クロロトリアゾール1.0gを使用する
以外は全て前記実施例１と同一の手順を施し、本発明の
１実施例品たる過酸化活性物質検出用の試験片〔３〕を
得た。
実施例４
前記実施例１における第２含浸溶液のサリチル酸 ｐ
−tert−ブチルフェニルエステルの代わりに、２−シア
ノ−3,3′−ジフェニルアクリル酸エチル1.0gを使用す
る以外は全て前記実施例１と同一の手順を施し、本発明
の１実施例品たる過酸化活性物質検出用の試験片〔４〕
を得た。
比較例
前記実施例１における第２含浸溶液中のサリチル酸
ｐ−tert−ブチルフェニルエステルを除去する以外は全
て前記実施例１と同一の手順を施し、比較のための過酸
化活性物質検出用の試験片〔５〕を得た。
実験１
前記試験片（１）〜（５）は、いずれも、体液中及び
排泄物中の過酸化活性物質、特に血液の検出を、100万
倍希釈の状態で行なうことができ、試料中の過酸化活性
物質検出用の試験片として十分に使用し得るものであっ
た。
実験２
前記試験片（１）〜（５）を、それぞれ、照度400ル
クス及び750ルクスの大気中に、１時間,2時間,4時間放
置し、その外観色の変化と感度変化とを測定した結果を
表にて示す。
尚、試験片（１）〜（５）の外観色の変化は、明度，
彩度，色相という３つの尺度からなる色立方の空間的な
位置によって決定される色座標の距離で表される外観色
に関与する数値を色差計で読み取り、各試験片の放置前
のものと放置後のものとの差（ΔＥ）で表示したもので
ある。
また、感度変化は、0.06mg/dlのヘモグロビン水溶液
を用いて感度（発色強度）測定し、各試験片の放置前の
ものの感度を100としたときの放置後の試験片の感度の
割合を示した。
〔発明の作用及び効果〕
本発明の過酸化活性物質検出用の試験片は、有機ヒド
ロペルオキシド，酸化呈色指示薬、及び紫外線吸収剤が
担持されている担体からなるものであり、試験片中にお
ける紫外線吸収剤の作用によって300〜400mμの領域に
ある紫外線が吸収され、そのエネルギーが主として無害
な熱エネルギーとして再輻射されるもので、紫外線吸収
剤自体には何らの変質も生ずることのないものである。
従って、本発明の試験片は、紫外線の照射によって生
ずる試験片の外観色の変化を阻止し、過酸化活性物質検
出性能の低下を防止する作用，効果が極めて有効に果さ
れるものである。Description: TECHNICAL FIELD The present invention relates to a test strip for detecting a peroxide active substance in a sample such as a body fluid or excrement. [Prior Art] Detection of peroxide active substances, especially blood contained in body fluids and excretions, for example, urine, vomit, gastrointestinal contents, feces, etc.
For example, it is used as one of the methods for quickly detecting diseases such as nephritis, tumor, hemorrhagic predisposition, and cystitis. However, usually, the blood content in a sample caused by the above-mentioned disease is extremely small, and It is almost difficult to distinguish them. For this reason, the method of microscopic observation of the sample has been adopted for the detection of blood in the sample, but this method is not only poor in reproducibility but also complicated in operation. It has the disadvantage that it cannot be processed. As a method for solving the drawbacks of the method by the microscopic observation, a method using a test piece composed of a carrier carrying an organic hydroperoxide and an oxidative color indicator, that is, contained in a sample such as a body fluid or excrement. A method has been adopted which uses the principle of decomposing hydroperoxide by the peroxidase-like activity of the peroxidation active substance, and causing the resulting oxygen to color the oxidative color indicator in the test piece. However, as the test piece for detecting a peroxide active substance, various kinds of test pieces have been proposed for increasing the sensitivity of the test piece and for stabilizing the test piece. For example, Japanese Patent Application Laid-Open No. 49-54093 discloses phosphoric acid amide as a stabilizer to add a substance intervening between an organic hydroperoxide and an oxidative color indicator present in a test piece. JP-A-53-115288 discloses that the stability between an organic hydroperoxide and an oxidative color indicator is improved by intervening a borate such as triethanolamine borate. Furthermore, JP-A-56-147066 discloses that By adding a stabilizer such as the above, it is possible to prevent the reaction between the organic hydroperoxide and the oxidative color indicator in the absence of the peroxide active substance, and to improve the stability over time. As a method for improving the stability between the organic hydroperoxide and the oxidative color indicator by completely isolating the existing organic hydroperoxide and the oxidative color indicator, for example, JP-A-39-1474
No. 7 discloses a method utilizing microencapsulation, and JP-A-51-65694 discloses a method utilizing a film-forming polymer. [Problems to be Solved by the Invention] By the way, in the above-mentioned conventional test piece for detecting a peroxide active substance, the test piece is generated between the organic hydroperoxide present in the test piece and the oxidation color indicator. Although the reduction in stability due to the oxidation-reduction reaction is effectively prevented, it cannot prevent discoloration or reduction in sensitivity due to the action of oxidizing substances (light, especially ultraviolet light) in the atmosphere, and this is a variation in quality. In addition to this, it has disadvantages such as lack of long-term storage performance. On the other hand, the test piece for detecting a peroxide active substance according to the present invention is stable in quality without discoloration and sensitivity decrease due to the action of oxidizing substances (in particular, ultraviolet rays) in the air, and has excellent long-term storage performance. It works. (Means for solving the problems) The test piece for detecting a peroxide active substance of the present invention is a carrier,
At least an organic hydroperoxide, an oxidative color indicator, and an ultraviolet absorber are supported, and in addition to the organic hydroperoxide, the oxidative color indicator, and the ultraviolet absorber, a general test piece of this type is used. A suitable carrier substance, for example, a buffer, a wetting agent, a sensitizer, etc., is carried as required. The test piece for detecting a peroxide active substance of the present invention having the above-mentioned structure is generally prepared by using the above-mentioned organic hydroperoxide, oxidative color indicator, and ultraviolet light on an absorbent carrier such as filter paper, cotton, nonwoven fabric, glass fiber or the like. The most common form is a structure in which an absorbent and other substances are carried, and this is attached to a base made of a plastic piece such as polyethylene, polypropylene or polystyrene. The organic hydroperoxide supported on the absorbent carrier may be liquid or solid.However, since hydroperoxide is generally unstable to light and heat, it is preferable to select a more stable organic hydroperoxide, for example, t
One or a mixture of two or more of -butyl hydroperoxide, paramethane hydroperoxide, cumene hydroperoxide, 2,5-dimethylhexane-2,5-dihydroperoxide and the like are used. The oxidative color indicator may be any as long as it develops a color by an oxidizing action, for example, ortho-tolidine, 3,3 ′, 5,5′-
Tetramethylbenzidine, benzidine, paradianisidine, orthodianisidine, 2,7-diaminofluorene and the like are used. Further, the main ones used as ultraviolet absorbers include salicylic acid-based ultraviolet absorbers phenyl salicylate, P-tert-butylphenyl salicylate, P-octylphenyl salicylate, benzophenone-based ultraviolet absorber 2,4-dihydroxybenzophenone, hydroxy -4-methoxybenzophenone, 2-hydroxy-4-octoxybenzophenone, 2,2'-dihydroxy-4-methoxybenzophenone, benzotriazole ultraviolet absorber 2- (2'-hydroxy-5'-methylphenyl) benzotriazole 2- (2'-hydroxy-5'-tert-butylphenyl) benzotriazole, 2- (2'-hydroxy-3 ', 5'-di-tert-butylphenyl) benzotriazole, 2- (2'- Hydroxy-3'-tert-butyl-5'-
Methylphenyl) -5-chlorobenzotriazole, cyanoacrylate-based UV absorber 2-ethylhexyl-2-cyano-3,3'-diphenylacrylate, ethyl-2-cyano-3,3'-phenylacrylate, and the like. The preparation of the impregnating liquid used when the carrier is carried on the absorbent carrier is as follows: 1 to 5% by weight of an organic hydroperoxide, 0.1 to 1.0% by weight of an oxidative color indicator, and 0.1% by weight of an ultraviolet absorber.
It is prepared to a concentration of about 0.5% by weight. Furthermore, a buffer which is a general carrier substance supported on the test strip for detecting a peroxide active substance of the present invention as required can sharply detect the peroxide substance by the test strip of the present invention. Citrate, phosphate, succinate, phthalate so that the pH of the impregnating solution containing the carrier substance can be maintained between 4 and 7, especially between 5 and 6. And the like. The wetting agent is, for example, for facilitating the penetration of the specimen into the test piece, or for stabilizing the radical cation generated by oxidation of the oxidative color indicator, and has both of these effects. As the wetting agent, for example, an anionic surfactant such as sodium lauryl sulfate, sodium dodecylbenzenesulfonate and sodium dioctylsulfosuccinate is used. Furthermore, sensitizers are used because of the requirement that the detection of peroxidatively active substances in body fluids and excretions, especially blood, can be detected even in blood diluted 1,000,000 times in clinical medicine. For example, quinoline derivatives, benzoquinoline derivatives, pyridine derivatives, 2-aminobenzothiazole derivatives and the like are used at 0.05 to 1.0% by weight in the impregnation liquid. The most common method of supporting a carrier substance on an absorbent carrier is to immerse a filter paper in a solution obtained by dissolving a predetermined amount of an organic hydroperoxide, a buffer, a sensitizer, and a wetting agent in a water-alcohol mixture, After drying this at 60 to 80 ° C., it is further impregnated with an impregnating liquid in which a predetermined amount of an oxidative color indicator and an ultraviolet absorber are dissolved, and this is a method of drying, in which a supporting substance is supported. An absorbent carrier, such as a piece of filter paper,
The test piece for detecting a peroxide active substance of the present invention can be easily obtained by sticking it to a predetermined plastic piece or the like. [Example] Hereinafter, a specific configuration of a test strip for detecting a peroxide active substance of the present invention will be described based on an example. Example 1 Filter paper for chromatography [Whatman (company): 3]
MM] with the following first impregnating solution, dried at 60 ° C. for 60 minutes, and then impregnated with the following second impregnating solution,
After drying at 20 ° C for 20 minutes, a double-sided tape was stuck on a plastic plate, cut into strips, and a 5 x 5 mm filter paper was placed at one corner of a base made of a plastic piece with a width of 5 mm and a length of 80 mm. A test piece [1] for detecting a peroxide active substance, which was affixed in one example of the present invention, was obtained. First impregnation solution (1) 1 molar citrate buffer ... 50 ml (pH 5.25) (2) Cumene hydroperoxide-β-cyclodextrin inclusion complex ... 12.5 g (3) Sodium lauryl sulfate ... 2.0 g ( 4) 2-aminobenzothiazole sulfate: 0.3 g (5) Ethanol: 30 ml prepared with distilled water to 100 ml. Second impregnation solution (1) Orthotrizine... 0.5 g (2) Polyvinylpyrrolidone... 1.5 g (K-90) (3) Salicylic acid p-tert-butylphenyl ester 1.0 g prepared in toluene to 100 ml . Example 2 Salicylic acid p of the second impregnating solution in Example 1
The same procedure as in Example 1 was carried out except that 1.0 g of 2-hydroxy-4-octoxybenzophenone was used in place of -tert-butylphenyl ester, and a peroxide active substance as an example of the present invention. A test piece [2] for detection was obtained. Example 3 Salicylic acid p of the second impregnating solution in Example 1
-Instead of tert-butylphenyl ester, 2-
Except that 1.0 g of (2'-hydroxy-3'-tert-butyl-5'-methylphenyl) -5-chlorotriazole was used, the same procedure as in Example 1 was carried out, and the product of Example 1 of the present invention was used. A test piece [3] for detecting a peroxidatively active substance was obtained. Example 4 Salicylic acid p of the second impregnation solution in Example 1 above
The same procedure as in Example 1 was carried out except that 1.0 g of ethyl 2-cyano-3,3'-diphenylacrylate was used instead of -tert-butylphenyl ester, and the product of Example 1 of the present invention was obtained. Test piece for detecting peroxide active substance [4]
I got Comparative Example Salicylic acid in the second impregnation solution in Example 1
Except for removing p-tert-butylphenyl ester, the same procedure as in Example 1 was performed to obtain a test specimen [5] for comparison with a peroxide active substance for comparison. Experiment 1 In each of the test pieces (1) to (5), the detection of a peroxide active substance in a body fluid and excrement, particularly blood, can be performed at a dilution of 1,000,000 times. The test piece was sufficiently usable as a test strip for detecting a peroxide active substance. Experiment 2 The test pieces (1) to (5) were allowed to stand in the atmosphere of illuminance of 400 lux and 750 lux for 1 hour, 2 hours, and 4 hours, respectively, and the change in appearance color and the change in sensitivity were measured. The results are shown in the table. Note that the change in the appearance color of the test pieces (1) to (5) was
Numerical values relating to the appearance color represented by the distance of the color coordinates determined by the spatial position of the color cubic, which is composed of three scales of saturation and hue, are read by a color difference meter, and the values before and after the test pieces are left untreated. This is indicated by a difference (ΔE) from that after leaving. The sensitivity change was measured using a 0.06 mg / dl aqueous hemoglobin solution (intensity of color development). Was. [Operation and Effect of the Invention] The test piece for detecting a peroxide active substance of the present invention is composed of a carrier on which an organic hydroperoxide, an oxidative color indicator, and an ultraviolet absorber are supported. UV rays in the range of 300 to 400 mμ are absorbed by the action of the UV absorber, and the energy is re-radiated mainly as harmless heat energy, and the UV absorber itself does not cause any deterioration. is there. Therefore, the test piece of the present invention has an extremely effective function and effect of preventing a change in appearance color of the test piece caused by irradiation of ultraviolet rays and preventing a decrease in the performance of detecting a peroxide active substance.
Claims (1)
線吸収剤が担持されている担体からなることを特徴とす
る過酸化活性物質検出用の試験片。 ２．紫外線吸収剤が、サリチル酸Ｐ−tert−ブチルフェ
ニルエステル、２−ヒドロキシ−４−オクトキシベンゾ
フェノン、２−（２′−ヒドロキシ−３′−tert−ブチ
ル−５′−メチルフェニル）−５−クロロベンゾトリア
ゾール及びエチル−２−シアノ−3,3′−フェニルアク
リレートよりなる群から選ばれる１種又は複数の紫外線
吸収剤である特許請求の範囲第１項記載の過酸化活性物
質検出用の試験片。 ３．検出対象の過酸化活性物質が尿中の血液であること
を特徴とする特許請求の範囲第１項又は第２項記載の過
酸化活性物質検出用の試験片。(57) [Claims] A test strip for detecting a peroxide active substance, comprising a carrier carrying an organic hydroperoxide, an oxidative color indicator and an ultraviolet absorber. 2. When the ultraviolet absorber is salicylic acid P-tert-butylphenyl ester, 2-hydroxy-4-octoxybenzophenone, 2- (2'-hydroxy-3'-tert-butyl-5'-methylphenyl) -5-chlorobenzo The test strip for detecting a peroxide active substance according to claim 1, which is one or more ultraviolet absorbers selected from the group consisting of triazole and ethyl-2-cyano-3,3'-phenylacrylate. 3. 3. A test strip for detecting a peroxide active substance according to claim 1, wherein the target substance to be detected is blood in urine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62238176A JP2660837B2 (en) | 1987-09-22 | 1987-09-22 | Test strip for detecting peroxide active substances |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62238176A JP2660837B2 (en) | 1987-09-22 | 1987-09-22 | Test strip for detecting peroxide active substances |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP35415996A Division JPH09292385A (en) | 1996-12-18 | 1996-12-18 | Stabilizing agent of test piece for active oxidant and stabilizing method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6479659A JPS6479659A (en) | 1989-03-24 |
| JP2660837B2 true JP2660837B2 (en) | 1997-10-08 |
Family
ID=17026303
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62238176A Expired - Fee Related JP2660837B2 (en) | 1987-09-22 | 1987-09-22 | Test strip for detecting peroxide active substances |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2660837B2 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3142862A1 (en) * | 1981-10-29 | 1983-05-11 | Behringwerke Ag, 3550 Marburg | "AGENT FOR DETECTING GLUCOSE IN BIOLOGICAL LIQUIDS" |
| US4755472A (en) * | 1986-01-16 | 1988-07-05 | Miles Inc. | Stable composition for the determination of peroxidatively active substances |
-
1987
- 1987-09-22 JP JP62238176A patent/JP2660837B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6479659A (en) | 1989-03-24 |
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