JP2567565Y2 - Culture equipment - Google Patents
Culture equipmentInfo
- Publication number
- JP2567565Y2 JP2567565Y2 JP2470492U JP2470492U JP2567565Y2 JP 2567565 Y2 JP2567565 Y2 JP 2567565Y2 JP 2470492 U JP2470492 U JP 2470492U JP 2470492 U JP2470492 U JP 2470492U JP 2567565 Y2 JP2567565 Y2 JP 2567565Y2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- container
- peripheral wall
- main body
- eggs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【0001】[0001]
【産業上の利用分野】本考案は、主として動物の卵、授
精卵の培養や卵操作に用いる培養用器具に関するもので
ある。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a culture instrument mainly used for culturing animal eggs and fertilized eggs and for manipulating the eggs.
【0002】[0002]
【従来の技術】一般に動物の卵の培養には、小型ペトリ
ディッシュ内の底面に複数の培養液の微小滴をつくり、
この微小滴の蒸発を抑えるため微小滴上をパラフィンオ
イルで覆い、微小滴中で卵を培養したり、卵操作をする
微小滴培養法が用いられる。この方法では実際の培養液
量に対して多量のパラフィンオイルが必要であり、又、
培養液の微小滴は崩れ易いため取扱いには細心の注意を
必要とする他、パラフィンオイル中への培養液成分の溶
け出しによる培地成分の変化を防止するため、あらかじ
め使用するパラフィンオイルを培養液と攪伴し飽和させ
るなどの前準備が必要である。2. Description of the Related Art In general, when culturing an animal egg, a plurality of microdrops of a culture solution are formed on a bottom surface of a small petri dish.
In order to suppress the evaporation of the microdroplets, a microdroplet culturing method is used in which the microdroplets are covered with paraffin oil, and the eggs are cultured in the microdroplets or the eggs are manipulated. This method requires a large amount of paraffin oil relative to the actual culture volume,
Since the microdrops of the culture solution are easily broken, care must be taken when handling them.In addition, in order to prevent changes in the medium components due to the dissolution of the culture solution components into the paraffin oil, use the paraffin oil to be used in advance. Preparations such as stirring and saturation are required.
【0003】又、卵や培養液の移し替えには、実体顕微
鏡下でパスツールピペットなどにより操作を行うが、微
小液滴がペトリディッシュ底面と側壁との隅に位置して
いる場合には、実体顕微鏡が邪魔をしてピペットが立て
られないため、ピペットの先端をその微小滴まで届かせ
ることができず、操作ができないと言う問題があった。
更に、培養液を交換するには、新しい培養液の液滴をパ
ラフィンオイル中につくり、古い培養液の中から卵をピ
ペット操作で取り出し、新しい培養液の液滴の中に卵を
移す操作を行うが、このとき新しい培養液の液滴と古い
培養液の液滴の区別がしにくく、いちいち、実体顕微鏡
で確認しながら操作をしなければならないため効率が悪
いと言う問題もあった。In order to transfer eggs and culture solution, an operation is performed using a Pasteur pipette or the like under a stereoscopic microscope. When a minute droplet is located at the corner between the bottom and the side wall of the Petri dish, Since the stereoscopic microscope hinders the pipette from standing, there is a problem in that the tip of the pipette cannot reach the microdroplet and the operation cannot be performed.
Furthermore, to change the culture medium, create a new culture medium droplet in paraffin oil, pipette the egg from the old culture medium, and transfer the egg into the new culture medium droplet. However, at this time, it is difficult to distinguish between the droplets of the new culture solution and the droplets of the old culture solution, and there is a problem that the efficiency is low because the operation must be performed while confirming with a stereoscopic microscope.
【0004】[0004]
【考案が解決しようとする課題】本考案は、ペトリディ
ッシュを用いた微小滴法におけるこのような欠点に鑑み
て考案されたもので、培養液の蒸発をおさえるためのパ
ラフィンの量を最小限に抑え、培養液が崩れることを心
配する必要がなく取扱が容易で、かつ卵の操作時のピペ
ット操作が容易で、培養液の交換を効率良く出来る培養
用器具を提供することを目的としたものである。SUMMARY OF THE INVENTION The present invention has been made in view of such a drawback in the microdrop method using a petri dish, and minimizes the amount of paraffin for suppressing evaporation of a culture solution. The purpose of the present invention is to provide a culture instrument which can be easily handled without having to worry about the collapse of the culture solution without having to worry about collapsing of the culture solution, the pipette operation is easy at the time of egg manipulation, and the culture solution can be exchanged efficiently. It is.
【0005】[0005]
【課題を解決するための手段】即ち本考案は、ペトリデ
ィッシュ状の容器の底部に、複数個のすり鉢状の凹部を
有する培養部を設けた本体部と、該本体部を覆う蓋とで
構成され、各培養部の頂部は前記容器の周壁の頂部より
低い位置にあり、また、各すり鉢状凹部の底面は前記容
器の底部と同じかもしくはそれより高い位置にあって、
平面状もしくは浅い球面状をなすと共に、すり鉢状凹部
の側断面形状は傾斜した内壁が直線状をなし、且つ容器
底面となす角度が30〜60度の範囲であることを特徴
とする培養用器具である。That is, the present invention comprises a main body in which a culture section having a plurality of mortar-shaped recesses is provided at the bottom of a Petri dish-shaped container, and a lid that covers the main body. The top of each culture section is at a position lower than the top of the peripheral wall of the vessel, and the bottom of each mortar-shaped recess is at the same or higher position as the bottom of the vessel,
A culturing device characterized by having a flat or shallow spherical shape, a side cross-sectional shape of a mortar-shaped concave portion, wherein an inclined inner wall forms a straight line, and an angle between the inner wall and the container bottom is in a range of 30 to 60 degrees. It is.
【0006】以下、図面により本考案を詳細に説明す
る。図1は本考案の培養用器具の一例を示す斜視図で、
(a)は蓋、(b)は培養部を有する本体部である。ま
た、図2は培養用器具本体部の構造の実施例を示す側面
断面図で、図3は複数個の培養部の配列の例を示す図で
ある。Hereinafter, the present invention will be described in detail with reference to the drawings. FIG. 1 is a perspective view showing an example of the culture instrument of the present invention.
(A) is a lid, (b) is a main body having a culture unit. FIG. 2 is a side sectional view showing an embodiment of the structure of the culture instrument main body, and FIG. 3 is a view showing an example of an arrangement of a plurality of culture units.
【0007】本考案の培養用器具本体部は、図1(b)
に示したように、ペトリディッシュ状の容器(2)の底
部に、すり鉢状の凹部(3)を有する培養部(4)を複
数個設けたもので、これに対して、図1(a)に示した
蓋(1)は、本体部の容器(2)全体にゆるく被さるよ
うになっている。培養部(4)の頂部は容器(2)の周
壁(5)の頂部より低い位置にあり、また、凹部(3)
の底面は容器(2)の底部と同じかもしくはそれより高
い位置にあり、平面状もしくは浅い球面状をなしてい
る。FIG. 1 (b) shows the culture instrument main body of the present invention.
As shown in Fig. 1, a plurality of culture sections (4) having a mortar-shaped recess (3) are provided at the bottom of a Petri dish-shaped container (2). Is loosely covered over the entire container (2) of the main body. The top of the culture section (4) is lower than the top of the peripheral wall (5) of the container (2), and the recess (3)
Is located at the same level or higher than the bottom of the container (2), and has a flat or shallow spherical shape.
【0008】そして、すり鉢状凹部(3)の傾斜した内
壁は断面形状が直線状をなしており、容器(2)の底面
に対してなす角度は30〜60度の範囲とするのが好ま
しい。この角度が60度より大きいと、実体顕微鏡下で
のピベット操作の際に実体顕微鏡が邪魔になって、ピペ
ットの先端が凹部(3)の底面の隅に届かないと言う問
題が生ずる。一方、角度が30度より小さい場合には、
凹部(3)のくぼみが浅くなって容量が小さくなり、充
分量の培養液を納めることができなくなる。The inclined inner wall of the mortar-shaped recess (3) has a linear cross section, and the angle formed with respect to the bottom of the container (2) is preferably in the range of 30 to 60 degrees. If this angle is larger than 60 degrees, the stereomicroscope hinders the operation of the pipette under the stereomicroscope, causing a problem that the tip of the pipette does not reach the bottom corner of the recess (3). On the other hand, if the angle is less than 30 degrees,
The depression of the concave portion (3) becomes shallow and the volume becomes small, so that a sufficient amount of culture solution cannot be stored.
【0009】尚、蓋(1)、容器(2)、および培養部
(4)はいずれもプラスチックの成形品であり、その材
質としては顕微鏡での確認ができる程度の透明性を有
し、破損しにくい、耐水性が良い、成形しやすいなどの
要件を備えていれば特に制限はない。このようなプラス
チックとしては、ポリスチレン、TPX樹脂、ポリカー
ボネート、アクリル樹脂などが挙げられる。The lid (1), the container (2), and the culturing section (4) are all molded plastics, and are made of a material that is transparent enough to be confirmed with a microscope and is damaged. There is no particular limitation as long as it has requirements such as difficulty in forming, good water resistance, and easy molding. Examples of such a plastic include polystyrene, TPX resin, polycarbonate, and acrylic resin.
【0010】また、容器(2)の底部に培養部(4)を
設ける方法は、容器(2)と培養部(4)をそれぞれ別
個に作っておき、図2(a)のように、接着剤や超音波
溶着等の方法で取付けても良く、あるいは、図2(b)
に示したような形状に、初めから一体に成形してもよ
い。[0010] The method of providing the culture section (4) at the bottom of the container (2) is such that the container (2) and the culture section (4) are separately formed, and as shown in FIG. It may be attached by a method such as an agent or ultrasonic welding, or FIG. 2 (b)
May be integrally formed from the beginning.
【0011】培養部(4)の形状は円形にするのが一般
的であるが、必ずしも円形に限定されるものではない。
一方、容器(2)の外形は長方形にするのが一般的で取
扱い易いが、これに限定されるものではなく、円形や長
円形等であっても何ら差しつかえはないが、スペース効
率の点からは長方形が好ましい。The shape of the culture part (4) is generally circular, but is not necessarily limited to a circular shape.
On the other hand, the outer shape of the container (2) is generally rectangular and easy to handle, but is not limited to this. Even if it is circular or oval, there is no problem, but space efficiency is not a problem. Therefore, a rectangle is preferable.
【0012】容器(2)内における複数個の培養部
(4)の配列方法は、図3(a)、(b)に示したよう
に1列でもよく、また、図3(c)、(d)のように培
養部の数を偶数個とし、2列に配列してもよく、使用目
的に応じて選択すればよいが、培養の途中で培養液の交
換を行なう場合には、2列に平行に配列させるのがよ
い。即ち、培養時には先ず第1列目の培養部で卵を培養
し、続いて培養液の交換をするには、2列目の培養部に
新しい培養液をみたして、1列目の培養部の中の卵をそ
のまま移せばよいので、新しい培養液と古い培養液を間
違えることがなく、培養液の交換が容易に、かつ効率よ
く行なえる利点がある。The method of arranging the plurality of culture sections (4) in the container (2) may be a single row as shown in FIGS. 3 (a) and 3 (b). As shown in d), the number of culture units may be an even number and arranged in two rows, and may be selected according to the purpose of use. It is good to arrange in parallel with. That is, at the time of culturing, first, the eggs are cultured in the culture section of the first row, and then, in order to exchange the culture solution, a new culture solution is seen in the culture section of the second row, and the culture section of the first row is changed. Since the eggs in the medium can be transferred as they are, there is an advantage that the culture medium can be easily and efficiently exchanged without making a mistake between the new culture medium and the old culture medium.
【0013】また、複数個の培養部は容器(2)の周壁
(5)に平行に配列するが、その列は、容器(2)の中
心部、あるいは2列の場合には対称になる位置である必
要はなく、周壁の一部(容器の外形が長方形の場合には
その1辺)に偏よっていてもよい。使用時の実体顕微鏡
下でのピペットでの操作性を考慮した場合、培養部
(4)と周壁(5)との間隔は10mm程度あけるのが
好ましい。これより間隔が小さいとピペットが実体顕微
鏡に当たってしまい操作がし難くいが、間隔が大きすぎ
てもスペースが無駄になる。また、培養部と周壁との間
のスペースは両側に設ける必要はなく、これらの諸点を
考慮したスペース効率とピペット操作性の点から、容器
全体の形状は長方形とし、培養部は容器の一方側の周壁
から10mm余りの間隔をあけて(即ち、図1(b)や
図3(c)、(d)に示したように、もう一方側の周壁
の近くに偏らせて)配列した構造が好ましい。The plurality of culture sections are arranged in parallel with the peripheral wall (5) of the vessel (2), and the rows are arranged at the center of the vessel (2), or in a symmetrical position in the case of two rows. It is not necessary to be limited to a part of the peripheral wall (one side when the outer shape of the container is rectangular). In consideration of operability with a pipette under a stereoscopic microscope at the time of use, it is preferable that the distance between the culture part (4) and the peripheral wall (5) is about 10 mm. If the interval is smaller than this, the pipette hits the stereomicroscope and operation is difficult, but if the interval is too large, space is wasted. In addition, the space between the culture unit and the peripheral wall does not need to be provided on both sides, and from the viewpoint of space efficiency and pipette operability taking these points into consideration, the shape of the entire container is rectangular, and the culture unit is located on one side of the container. Are arranged at an interval of about 10 mm from the peripheral wall of (i.e., as shown in FIG. 1B, FIG. 3C, and FIG. 3D). preferable.
【0014】次に、本考案の培養用器具の使用方法につ
いて説明する。先ず、容器(2)内の、培養部(4)の
外側に水を満たす。この時、水の温度は培養部(4)に
満たす培養液を保温するため、37℃とするのが望まし
い。その後、培養液の適当量を凹部(3)に満たして、
37℃に設定してある熱盤上にのせ、採取した卵を培養
液中に移し、ピペット操作などにより卵の洗浄などの操
作を行う。卵を培養するときは、蓋(1)を被せてイン
キュベーター中に納める。Next, the method of using the culture device of the present invention will be described. First, the outside of the culture part (4) in the container (2) is filled with water. At this time, the temperature of the water is desirably 37 ° C. in order to keep the culture solution filled in the culture section (4) warm. Thereafter, an appropriate amount of the culture solution is filled in the concave portion (3),
The eggs are placed on a hot plate set at 37 ° C., the collected eggs are transferred into a culture solution, and operations such as washing the eggs are performed by pipetting. When culturing eggs, cover the lid (1) and place in an incubator.
【0015】容器(2)の培養部(4)外側に水を満た
すことにより、適度な湿気が凹部(3)中の培養液に補
給されるため培養液の蒸発を抑えることが出来、従来の
ようにパラフィンオイルで培養液を覆う必要はない。こ
のような観点から、容器(2)の内面積に占める培養部
(4)の面積(培養部頂部の内面積)の割合について検
討すると、培養部面積の割合は必ずしも厳密なものでは
ないが、20〜45%程度とするのが良く、30%前後
とするのが好適である。この割合を小さくすると培養液
の蒸発を抑えるためには好ましいが、20%以下ではス
ペース効率が悪くなり、また、45%を越えると培養液
の蒸発を抑える効果が不足して来る。By filling the outside of the culture section (4) of the container (2) with water, moderate moisture is supplied to the culture medium in the recess (3), so that evaporation of the culture medium can be suppressed. There is no need to cover the culture with paraffin oil as it does. From such a viewpoint, when examining the ratio of the area of the culture part (4) to the inner area of the container (2) (the inner area of the top part of the culture part), the ratio of the culture part area is not always strict. It is preferably about 20 to 45%, and more preferably about 30%. If this ratio is reduced, it is preferable to suppress the evaporation of the culture solution. However, if the ratio is less than 20%, the space efficiency deteriorates, and if it exceeds 45%, the effect of suppressing the evaporation of the culture solution becomes insufficient.
【0016】[0016]
【考案の効果】本考案による培養用器具を使用すること
により、動物の卵の培養における培養液の蒸発を抑える
ためにパラフィンオイルで培養液を覆う必要がなくな
り、そのためパラフィンオイル中で培養液が崩壊するこ
とがないため取扱が容易になり、また、卵の洗浄などの
ピペットでの操作も容易で、ピペットの先端を培養部の
隅々まで届かせることができ、さらに、培養途中での培
養液の交換も同じプレート内で容易に行なえ、動物の卵
の培養に使用する培養用器具として好適である。[Effect of the Invention] By using the culture device according to the present invention, it is not necessary to cover the culture solution with paraffin oil in order to suppress the evaporation of the culture solution in culturing the egg of an animal. It is easy to handle because it does not collapse, and it is easy to operate with a pipette such as washing eggs, so that the tip of the pipette can reach every corner of the culture part, and furthermore, culture during culture The solution can be easily exchanged in the same plate, and is suitable as a culture instrument used for culturing animal eggs.
【図1】本考案の一実施例となる培養用器具の斜視図
で、(a)は蓋、(b)は培養部を設けた容器本体部を
示す。FIG. 1 is a perspective view of a culture device according to an embodiment of the present invention, wherein (a) shows a lid, and (b) shows a container body provided with a culture unit.
【図2】培養用器具の本体部構造の実施例を示す側面断
面図である。FIG. 2 is a side sectional view showing an embodiment of a main body structure of the culture instrument.
【図3】本考案による培養用器具の複数個の培養部の配
列の例を示す図である。FIG. 3 is a view showing an example of an arrangement of a plurality of culture units of the culture device according to the present invention.
1 蓋 2 容 器 3 凹 部 4 培養部 5 周 壁 DESCRIPTION OF SYMBOLS 1 Lid 2 Container 3 Concave part 4 Culture part 5 Perimeter wall
Claims (3)
数個のすり鉢状の凹部を有する培養部を設けた本体部
と、該本体部を覆う蓋とで構成され、各培養部の頂部は
前記容器の周壁の頂部より低い位置にあり、また、各す
り鉢状凹部の底面は前記容器の底部と同じかもしくはそ
れより高い位置にあって、平面状もしくは浅い球面状を
なすと共に、すり鉢状凹部の側断面形状は傾斜した内壁
が直線状をなし、且つ容器底面となす角度が30〜60
度の範囲であることを特徴とする培養用器具。1. A petri dish-shaped container comprises a main body provided with a culture section having a plurality of mortar-shaped recesses on a bottom thereof, and a lid for covering the main body. It is located at a position lower than the top of the peripheral wall of the container, and the bottom surface of each mortar-shaped recess is at the same position or higher than the bottom of the container, and has a flat or shallow spherical shape. The side cross-sectional shape is such that the inclined inner wall forms a straight line, and the angle formed with the container bottom is 30 to 60.
A culture instrument having a range of degrees.
行に配列されていることを特徴とする、請求項1記載の
培養用器具。2. The culture instrument according to claim 1, wherein a plurality of culture units are arranged in parallel with a peripheral wall of the container.
容器の周壁の一部に沿って2列に配列されていることを
特徴とする、請求項1記載の培養用器具。3. The culture device according to claim 1, wherein the plurality of culture units are an even number, and are arranged in two rows along a part of the peripheral wall of the container.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2470492U JP2567565Y2 (en) | 1992-04-17 | 1992-04-17 | Culture equipment |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2470492U JP2567565Y2 (en) | 1992-04-17 | 1992-04-17 | Culture equipment |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH0582300U JPH0582300U (en) | 1993-11-09 |
JP2567565Y2 true JP2567565Y2 (en) | 1998-04-02 |
Family
ID=12145573
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2470492U Expired - Lifetime JP2567565Y2 (en) | 1992-04-17 | 1992-04-17 | Culture equipment |
Country Status (1)
Country | Link |
---|---|
JP (1) | JP2567565Y2 (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP5984998B2 (en) * | 2015-04-08 | 2016-09-06 | 株式会社日立製作所 | Culture substrate |
-
1992
- 1992-04-17 JP JP2470492U patent/JP2567565Y2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JPH0582300U (en) | 1993-11-09 |
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