JP2020003805A - Display member, culture vessel and culture vessel kit - Google Patents

Abstract

To provide a display member capable of preventing a volatile component of a display part for displaying information on a culture vessel from having an influence on a content of the culture vessel.SOLUTION: A display member 20 includes: a display part 21 having a laser printing layer printable by a laser beam; and an encapsulation member 22 at least partially having transparency that makes information readable, and encapsulating the display part 21.SELECTED DRAWING: Figure 1C

Description

  The present invention relates to, for example, a display member attached to a culture container for displaying information, a culture container having a display unit for displaying information, and a culture container kit including the culture container and the display member.

  For example, in the field of regenerative medicine, a tissue or a cell collected from a patient is examined, cultured, and transplanted again into the body of the individual. In such a culture container used for testing or culturing a tissue or a cell, prevention of contamination of the contents and prevention of mixing of a sample are important issues. As an example of a means for solving such a problem, a seal used for a 96-well microplate is known (see Patent Document 1 below).

  Patent Literature 1 discloses a seal in which a 96-well microplate seal is provided with a circle concentric with the 96-well microplate, and the adhesive is removed from a part of the inside of the seal with a circle, and a seal is provided. It describes that numbers from 1 to 96 are printed inside the circle. Patent Document 1 states that the use of the seal prevents glue from adhering to the sample injection needle, prevents contamination of the buffer solution or the like, and facilitates sample search.

  In addition, a cell preparation management method and a material kit for the purpose of quickly and accurately managing raw materials without mistakes and avoiding danger to a living body beforehand, and associating culture information with culture information of a donor. There has been disclosed a culture management system that aims to perform the operation reliably (see Patent Documents 2 and 3 below). Patent Literature 2 describes attaching an IC tag to a material kit such as a dish or a centrifuge tube. Patent Document 3 describes attaching an RFID label to the outer surface of a culture vessel.

Japanese Utility Model Registration No. 3063248 JP 2008-220316 A JP 2011-45259 A

  In a culture container having an open opening as described in Patent Documents 1 to 3, an oil-based pen is used to display characters or the like on a display portion such as a surface or a seal of the culture container in order to display identification information or the like of a specimen. If the information is filled in or printed by a printer, components volatilized from the ink may adversely affect the contents of the culture container.

  The present invention has been made in view of the above problems, and a display member, a culture container, and a display member that can prevent a volatile component from a display unit that displays information on a culture container from affecting the contents of the culture container. It is an object of the present invention to provide a culture container kit comprising:

  In order to achieve the above object, the display member of the present invention is a display member attached to a culture container, and a display portion including a laser printing layer that can be printed by a laser, and at least a part of the display portion is printed on the display portion. A sealing member that seals the display unit with transparency so that information can be read.

  In the display member, the sealing member includes a first sealing portion and a second sealing portion, and the display portion is disposed between the first sealing portion and the second sealing portion. The first sealing section and the second sealing section may be joined to each other around the display section to seal the display section.

  In the display member, at least one of the first sealing portion and the second sealing portion may have transparency for transmitting the laser.

  In the display member, at least one of the first sealing portion and the second sealing portion may have transparency so that information displayed on the display portion can be read.

  In the display member, the first sealing portion and the second sealing portion may be joined via a joining portion formed by integrating them.

  In the display member, the first sealing portion and the second sealing portion may have a thickness solidified between the first sealing portion and the second sealing portion of 0.01 μm or more and 5 μm or less. May be joined via an adhesive layer of the above.

  Further, the culture container of the present invention includes a container body having an open opening, a display unit for displaying information on the container body, and a sealing member for sealing the display unit, I do.

  In the culture container, the display unit is disposed between the container body and the sealing member, and the sealing member is joined to the container body and between the container body and the sealing member. The display unit may be sealed. Further, in the culture container, the sealing member may be a part of the container body.

  In the culture container, the display unit may be configured by a laser printing layer that can be printed by a laser, and at least one of the container body and the sealing member may have transparency that transmits the laser.

  Further, the culture container of the present invention has a container main body having an open opening, a lid member for closing the opening of the container main body, a display unit for displaying information on the lid member, and sealing the display unit. And a sealing member for stopping. In addition, the display unit is disposed between the lid member and the sealing member, the sealing member is joined to the lid member, the display unit between the lid member and the sealing member. It may be sealed.

  Further, the culture container kit of the present invention is a culture container kit including the display member and a culture container, wherein the culture container has a fixing portion for fixing the display member.

  According to the display member, the culture container, and the culture container kit of the present invention, the display portion for displaying information is sealed by the sealing member, so that volatile components from the display portion affect the contents of the culture container. Can be prevented.

FIG. 1 is a perspective view showing a culture container and a display member according to a first embodiment of the present invention. The top view of the culture container and the display member shown in FIG. 1A. Sectional drawing of the culture container and display member along line CC shown in FIG. 1B. The enlarged sectional view of the display member shown in FIG. 1C. FIG. 9 is a sectional view showing an example of a display member according to the second embodiment of the present invention. FIG. 13 is a sectional view showing another example of the display member according to the second embodiment of the present invention. FIG. 9 is a sectional view showing a display member according to a third embodiment of the present invention. The expanded sectional view showing the culture vessel concerning Embodiment 4 of the present invention. The top view showing the state at the time of assembly of the culture container kit concerning Embodiment 5 of the present invention. Sectional drawing of the culture container kit along the BB line shown in FIG. 6A. It is a top view showing the culture container concerning Embodiment 6 of the present invention. Sectional drawing of the culture container along the BB line shown in FIG. 7A.

  Hereinafter, the display member, the culture container, and the culture container kit of the present invention will be described with reference to the drawings. In the following drawings, the scale of each part may be appropriately changed and illustrated in the same drawing in order to easily explain the configuration of the present invention.

[Embodiment 1]
FIG. 1A is a perspective view of the culture container 1 and the display member 20 according to Embodiment 1 of the present invention. FIG. 1B is a plan view of the culture container 1 and the display member 20 shown in FIG. 1A. FIG. 1C is a cross-sectional view of the culture container 1 and the display member 20 along the line CC shown in FIG. 1B.

  The culture vessel 1 of the present embodiment is, for example, a culture vessel such as a culture dish used for examination and culture of tissues and cells. More specifically, for example, specimen management such as blood for in vitro diagnosis, food This is a culture container that can be used for sample management for hygiene inspections. The culture vessel 1 includes a vessel body 10 having an open opening 11 and a display member 20 for displaying information on the vessel body 10. Further, the culture container 1 of the present embodiment includes a lid member 40 that closes the opening 11 of the container body 10.

  The container body 10 is usually made of a transparent material that transmits visible light, such as glass, quartz, or a resin material. The transparent resin material is not particularly limited. For example, polymethyl methacrylate (PMMA), polycarbonate (PC), polystyrene (PS), polyethylene terephthalate (PET), polypropylene (PP), polyethylene (PE), polyethylene na Phthalate (PEN), cycloolefin polymer (COP), cycloolefin copolymer (COC) and the like can be used. In addition, from the viewpoint of facilitating surface treatment suitable for cell culture, the container body 10 is preferably made of PS.

  More specifically, the culture container 1 of the present embodiment is, for example, a culture dish used for culturing a plurality of fertilized eggs, and an observation unit 12 is provided at the center of the container body 10. The observation unit 12 is an area surrounded by the partition 13 that holds the culture solution. The partition wall 13 is formed in a cylindrical shape surrounding the observation unit 12, and an upper portion is open. The observation section 12 is formed with a plurality of fine wells 14 for receiving fertilized eggs one by one. The storage section 15 of the container body 10 stores, for example, a liquid such as oil.

  Four or more, preferably six or more, more preferably eight or more wells 14 are formed adjacent to each other. At least four wells 14 may be formed in close proximity, and wells 14 that are not in close proximity may be separately formed. In addition, a plurality of groups of wells 14 formed close to each other may be arranged in plural groups, and the groups may not be close to each other.

  The well 14 has a wall surface and an opening, and the shape of the outer edge of the opening is not particularly limited, but is preferably circular (including circular and elliptical). The pitch between adjacent wells 14 is 1 mm or less. However, the pitch varies depending on the type of cells to be accommodated.

  For example, when accommodating bovine fertilized eggs, the pitch between adjacent wells 14 is usually 1 mm or less, preferably 0.7 mm or less, and more preferably 0.45 mm or less. As an observation device, a device provided with a 1/2 inch CCD device, an objective lens of 4, 10 and 20 times is often used. In such an observation apparatus, the observable visual field when a 4 × objective lens is selected is approximately 1.6 mm × 1.2 mm, and the observation visual field is designed to include four or more wells 14. Is preferred. Assuming that the diameter of the well 14 is larger than the diameter of the bovine fertilized egg (0.25 mm) and 0.3 mm, if the pitch is 0.9 mm or less, 2 × 2 = 4 in a 1.6 mm × 1.2 mm microscope field of view. When the pitch is 0.65 mm or less, 3 × 2 = 6 fertilized eggs can be observed in a 1.6 mm × 1.2 mm microscope visual field, and the pitch is 0. In the case of 0.43 mm or less, 4 × 3 = 12 fertilized eggs can be observed in a 1.6 mm × 1.2 mm microscope visual field. By designing so that four or more wells 14 are included in the observation visual field, for example, when performing automatic discrimination of cultured cells, a large number of cells can be processed at one time, and one fertilized egg in the visual field can be processed. Since comparison and determination with other fertilized eggs is facilitated, the determination operation can be performed more efficiently.

The pitch between the wells 14 is the distance between the centers of adjacent wells 14. The center of the well 14 is the position of the center of gravity of the figure formed by the outer edge of the opening of the well 14, and if the outer edge is circular, the center of the circle. The pitch between the wells 14 usually indicates an average pitch, and the average pitch for a certain concave portion is calculated by calculating an average value from the pitches with all adjacent wells 14. The pitch between the wells 14 is larger than the dimension of the outer edge of the opening of the well 14. The dimension of the outer edge of the opening of the well 14 is the diameter if the outer edge of the opening is circular. In other words, the adjacent wells 14 are arranged at a density of 1 or more, preferably 4 or more per 1 mm ² . The four or more adjacent wells 14 are preferably arranged in a square lattice shape or a close-packed shape. For example, there is a case where 25 wells 14 are arranged in a 5 × 5 square lattice. By arranging the cells in a square lattice shape or a close-packed shape, the position of each well 14 on the bottom wall of the culture vessel can be easily specified, and can be easily applied to an automatic process. Further, the number of the wells 14 is preferably 24 or more, more preferably 50 or more, in total per one culture vessel. By providing many wells 14 per culture vessel, the number of culture vessels required for culturing a certain number of cells can be reduced, which is economically advantageous.

  When the outer edge of the opening of the well 14 is circular, the pitch between the adjacent wells 14 is X + m + n (where X represents the maximum diameter of the cell, and m is the circle formed by the outer edge of the opening of the well 14). It represents the length obtained by subtracting the maximum diameter of the cell from the diameter, and n represents the length of the partition between the wells 14). The partition between the wells 14 refers to the shortest distance between the outer edges between the adjacent wells 14. Here, m is usually 0.1 mm or less, preferably 0.07 mm or less, more preferably 0.05 mm or less, and n is usually 0.6 mm or less, preferably 0.35 mm or less, more preferably 0.15 mm or less. is there.

  By arranging the wells 14 densely at the pitch as described above, many cells can be cultured at the same time while individually managing the cells. Further, since many cells enter one view of the microscope, many cells can be Images can be acquired.

  The opening of the well 14 has an opening width capable of accommodating cells. Here, the opening width of the opening of the well 14 refers to the length of the shortest diameter of the figure formed by the outer edge of the opening of the well 14. Thus, if the outer edge of the opening of the well 14 is circular, the opening width is equal to the diameter of the circle, which diameter is larger than the largest dimension of the cells to be cultured. When culturing a fertilized egg in the culture container of the present invention, it is desirable to culture up to the blastocyst stage, so that the diameter of the circular opening is larger than the maximum size of the cell at the blastocyst stage. Is desirable. Since the largest dimension of cells at the blastocyst stage is usually 100 μm to 280 μm, the diameter of the circular opening is usually 100 μm or more. Further, as described above, the dimension of the outer edge of the opening of the well 14 (the average value of the maximum diameter and the shortest diameter of the figure formed by the outer edge of the opening) is smaller than the pitch between the wells 14. If the outer edge of the opening is circular, its diameter is shorter than the pitch between the wells 14, ie, the distance between the centers of the circle, and is typically less than 1 mm. The opening width and size vary depending on the type of cells to be accommodated, but usually, the opening width of the opening of the well 14 is, for example, preferably 100 μm to 600 μm, more preferably 100 μm to 400 μm.

  For example, in the case of a bovine fertilized egg, the opening width of the opening of the well 14 (the diameter when the well 14 is circular) is usually 0.25 mm or more, preferably 0.26 mm or more, more preferably 0.27 mm or more. Yes, usually less than 1 mm, preferably less than 0.7 mm, more preferably less than 0.45 mm. Further, the opening width of the opening of the well 14 can be defined as X + m (where X represents the maximum diameter of the cell). Here, m is preferably 0.01 mm or more, more preferably 0.02 mm or more.

 The wall surface of the well 14 of the cell accommodating portion has an inclined surface that becomes higher from the lowest position of the well 14 toward the outer edge of the well 14. The well 14 of the cell accommodating part is usually formed so that the bottom comes to the bottom wall side of the culture vessel. The shape (profile) of the inclined surface of the well 14 can be appropriately adopted, for example, when the height increases in a curve from the lowest position of the well 14 toward the outer edge of the well 14, or when the height increases stepwise. It is preferable to include a straight portion, that is, an inclined surface in which the whole section or a part of the path is linearly higher as it goes from the lowest position of the well 14 to the outer edge of the well 14. By including the linear portion, the movement of the cells arranged in the well 14 is suppressed, and the cells are easily fixed to the deepest part of the well 14. Therefore, a clear image can be obtained when observed with a microscope.

  If the surface roughness of the inclined surface is a large value, a clear outline may not be obtained due to unevenness on the inclined surface when an image subjected to transmission observation with a microscope is subjected to outline extraction processing. Therefore, it is preferable that the value be as small as possible. Specifically, the maximum height Ry (only the reference length is extracted from the roughness curve in the direction of the average line, and the distance between the peak line and the valley line at the extracted portion) is less than 1.0 μm, particularly 0 μm. It is preferably less than 0.5 μm. The surface roughness of the inclined surface can be reduced by increasing the processing accuracy of the mold, for example, by performing a polishing treatment when producing the mold of the culture vessel.

  The wall of the well 14 preferably includes a conical or frustoconical portion. The conical or frusto-conical portion is formed on the bottom wall side of the culture vessel so that the smaller the area between the apex of the cone or the upper and lower surfaces of the truncated cone comes. Conical shapes include cones and elliptical cones, and similar shapes, for example, a cone or elliptical cone with a rounded vertex, a conical surface bulging outward, a conical surface concave inward Shape and the like are included. The truncated cone includes a truncated cone and a truncated elliptical cone, and similar shapes, for example, a shape in which the junction between the upper or lower surface of the truncated cone or the truncated ellipse and the conical surface is rounded, and the conical surface is outward. And a shape in which the conical surface is depressed inward. When the wall surface of the well 14 of the cell accommodating portion includes a truncated cone portion, the smaller diameter of the upper and lower surfaces of the truncated cone facing the bottom wall of the culture vessel is smaller than the maximum diameter of the cells. Is preferred. Specifically, it is preferable that the diameter of the smaller area is one half or less of the maximum diameter of the cell. When the ratio is less than half, the cells are easily arranged at the center of the well 14, the movement is suppressed, and the imaging of the cells is facilitated. In particular, the diameter of the smaller area is preferably 10 μm or less. When the diameter is 10 μm or less, the center area, which is difficult to observe due to the influence of light refraction and scattering, becomes narrower at the time of photographing, and a clear image can be obtained, which is effective. In the conical and frustoconical portions of the well 14, the conical surface is a curved surface, and preferably does not include a flat surface. The wall surface of the well 14 of the cell container usually has the above-described inclined surface, preferably a conical or truncated conical portion, on the bottom wall side of the culture vessel. The wall surface of the well 14 of the cell storage section may have a wall surface perpendicular to the bottom wall of the culture vessel on the opening side from the above-described inclined surface.

  When the wall surface of the well 14 includes a conical or truncated conical portion, the angle between the center line of the cone or truncated cone and the generating line is usually 89 to 45 °, preferably 88 to 65 °, more preferably 85 to 85 °. 80 °. By setting the angle to a certain angle or more, it is easy to move cells to the place where the cells are to be placed (the deepest part) using gravity as a driving source. And scattering is less likely to occur, and a clear observation image can be obtained.

  When the well 14 of the cell storage unit has a bottom surface parallel to the bottom wall of the culture container and a side surface perpendicular to the bottom wall, the cells may move in the well 14 and come into contact with the side surface. Is performed, it is difficult to extract an image of a cell by a contour extraction process in a captured image. However, the wall surface of the well 14 When having an inclined surface that becomes higher toward the outer edge, preferably in the case of including a conical or frusto-conical portion, the cells to be cultured will automatically be present at the bottom of the well 14, Even if 14 has a side surface perpendicular to the bottom wall of the culture vessel on the opening side from the inclined surface, it does not remain in contact with this, and the contour extraction processing of the image of the imaged cell can be performed without any problem. Real It can be.

  In addition, the depth of the well 14 of the cell storage unit is not particularly limited, but if it is too shallow, the cells move during transportation of the culture container or at the time of cell division, and the cells move out of the range of the well 14. Since the cells may come out, they are appropriately set so that the cells can be reliably held in the well 14. For example, in order to hold the cells in the well 14, the depth is preferably at least 1/3 of the maximum diameter of the cells, more preferably at least 1/2. On the other hand, if it is too deep, it becomes difficult to introduce a culture solution or cells into the well 14. Therefore, the value is appropriately set so that the value is not too deep while the cells are kept in the well 14. For example, the upper limit of the depth can be three times or less the diameter of the opening of the well 14. Further, in order to facilitate the introduction of the culture solution, the depth is preferably equal to or less than one time the diameter of the well 14, and particularly preferably equal to or less than 1/2. In addition, since the convection is less likely to occur as the diameter and the depth of the well 14 are smaller and deeper, the composition of the surrounding culture solution may easily change due to cell respiration and metabolism. Since the growth of cells is affected by the composition of the surrounding culture solution, the diameter and depth are preferably set in consideration of the biological influence so as to promote cell growth. Generally, the depth of the well 14 is preferably about 50 μm to 200 μm, although it depends on the type of cells. For example, in the case of a bovine fertilized egg, since the maximum diameter is about 250 μm, the depth is preferably 80 μm or more, more preferably 125 μm or more. This depth refers to the depth measured vertically from the opening to the deepest part in the well 14 having an inclined surface and, if present, a side surface perpendicular to the bottom wall of the culture vessel.

  Four or more wells 14 formed close to each other may be separated from other parts in the culture vessel by partition walls 13 surrounding them. For example, when a group of a plurality of wells 14 is present on the bottom wall of the culture vessel, each group is surrounded by the partition wall 13. Usually, in culturing fertilized eggs or the like, drying of the medium is prevented by forming droplets of a medium containing the fertilized eggs in a culture vessel and covering the droplets with oil. By surrounding the group of four or more wells 14 formed close to each other with the partition wall 13, the medium can be accommodated in the inside (center side) and the dispersion of the medium can be prevented. The same applies when the medium is covered with an oil such as a mineral oil.

  Further, the wells 14 formed close to each other may have grooves so that the culture media inside the wells 14 can communicate with each other. The presence of the grooves allows the medium to circulate between the wells 14 during cell culture, and enhances the growth of the cells contained in the wells 14 by the autocrine / paracrine effect.

  The culture container of the present invention may be provided with a surface treatment or a surface coat that promotes the growth of fertilized eggs. In particular, when co-cultured with cells of other organs (eg, endometrial cells or fallopian tube epithelial cells) in order to promote the development of fertilized eggs, these cells must be adhered to a culture vessel in advance. . In such a case, it is advantageous to coat the surface of the culture vessel with a cell adhesive material.

  Further, the configuration of the culture vessel 1 is not limited to the configuration shown in FIGS. 1A and 1B. For example, as in a culture vessel described in Japanese Patent Application No. 2014-136191, in a housing portion 15 outside the partition wall 13 surrounding the well 14, a cylindrical peripheral wall rising from the bottom surface inside the container body 10, and a peripheral wall. A second accommodating portion including a plurality of enclosed concave portions may be provided. In this case, a liquid mass of a culture solution is formed in each of the observation section 12 inside the partition wall 13 in which the well 14 is formed and the second storage section surrounded by the peripheral wall, and cell culture in the well 14 is performed. It is easy to proceed with the cell culture in the concave portion in parallel. Further, the partition wall 13 has a cylindrical outer peripheral surface and a mortar-shaped truncated conical inner peripheral surface, and the cross-sectional shape may be a wedge shape or a right triangle.

  FIG. 2 is an enlarged sectional view of the display member 20 shown in FIG. 1C.

  The display member 20 of the present embodiment is a display member 20 attached to the culture container 1, and has a display portion 21 formed of a laser printing layer that can be printed by a laser, and at least a part of the display portion 21 can read information of the display portion 21. A sealing member 22 for sealing the display unit 21 with transparency. In the present embodiment, the display member 20 is attached to the outer bottom surface of the container body 10. In addition, the position where the display member 20 is attached to the container body 10 is not limited to the outer bottom surface of the container body 10, but the outer side surface of the container body 10, the inner bottom surface of the container body 10, the inner side surface of the container body 10, It may be attached to a lid or the like that closes the opening 11 of the container body 10.

  The display unit 21 is a portion on which information such as the name of a patient who provided a specimen such as a fertilized egg, a patient identification number such as a chart number or a culture control number, a specimen name, a date, and a processing step name is displayed. These pieces of information are displayed on the display unit 21 as visually or optically readable information such as characters, numbers, symbols, dot patterns, bar codes, and two-dimensional bar codes.

  The display unit 21 is configured by a laser printing layer that can be printed by irradiating a laser after sealing with the sealing member 22. As the laser printing layer constituting the display unit 21, for example, a gravure ink manufactured by Toyo Ink Co., Ltd., or an ink for laser printing that changes color by laser irradiation, such as Elbima Z117, can be used. It should be noted that being printable with a laser means that, by irradiating the display unit 21 with a laser, for example, a part of the display unit 21 is discolored, or a part of the display unit 21 disappears to form a concave portion, or The first layer of the display unit 21 disappears and the second layer of a different color is exposed, or the sealing member 22 or the culture vessel 1 is exposed in a portion where the display unit 21 has partially disappeared. This means that information can be displayed on the display unit 21 with a readable contrast. In the present embodiment, the display unit 21 changes color by laser irradiation.

  Here, the readable contrast may be, for example, a definition of a PCS value or an MRD value in a barcode. In addition, the reflectance of a portion that has been changed to black by laser printing is RD, and the reflectance of a white portion of the display unit 21 itself or a portion of the display unit 21 that has disappeared due to laser irradiation and the white portion of a newly exposed portion is RL. In this case, when the PCS value = (RL−RD) / RL × 100 (%), a state in which the PCS value is 10% or more, more preferably 25% or more, and still more preferably 50% or more can be read. May be defined as a high contrast.

  In addition, the maximum reflectance of the portion that has been changed to black by laser printing is RDmax, and the minimum reflectance of the white portion of the display portion 21 itself and the portion of the display portion 21 that has disappeared due to laser irradiation and the white portion of the newly exposed portion is In the case where RLmin is set, MRD value = RLmin−RDmax, and a state in which the MRD value becomes 10% or more, more preferably 20% or more, and still more preferably 37.5% or more is defined as a readable contrast. May be. As another contrast parameter, a state where ECmin is 10% or more, more preferably 15% or more when the minimum edge contrast (ECmin) is the minimum MRD value may be defined as a readable contrast. Further, the symbol contrast (SC) is defined as a value obtained by subtracting the reflectance RDmin of the darkest part from the reflectance RLmax of the brightest part of the symbol, and expressing the symbol maximum reflectance difference (EC = RLmax−RDmin). Alternatively, it may be an index of a readable contrast.

  At least a part of the sealing member 22 is made of a transparent resin material capable of reading information displayed on the display unit 21. Here, the transparency with which information can be read means not only that the information can be visually recognized, but also that the readable contrast described above using an optical device such as a camera or a reading device can be sufficiently obtained. Means recognizable transparency. For example, in terms of transmittance, a sealing member having a transmittance of 30% or more, more preferably 50% or more, further preferably 75% or more, and still more preferably 80% or more can be used. As such a resin material having transparency, the same resin material as that of the container body 10 described above can be used.

  The sealing member 22 includes a first sealing portion 22a and a second sealing portion 22b. The first sealing portion 22a and the second sealing portion 22b may be made of the same material or different materials. The display unit 21 is disposed between the first sealing unit 22a and the second sealing unit 22b that are stacked so as to face each other. The surfaces of the first sealing portion 22a and the second sealing portion 22b facing the display portion 21 may be flat, but the display portion 21 is accommodated in at least one of the first sealing portion 22a and the second sealing portion 22b. May be formed.

  At least one of the first sealing portion 22a and the second sealing portion 22b may be made of a transparent resin material capable of reading information displayed on the display unit 21. For example, when the container body 10 has transparency that allows information displayed on the display unit 21 to be read, at least the second sealing portion 22b joined to the container body 10 has the same transparency as the container body 10. , The information displayed on the display unit 21 can be read from above the container body 10 through the bottom of the container body 10. When the container main body 10 does not have transparency, the first sealing portion 22a on the opposite side to the container main body 10 has transparency that can read information displayed on the display portion 21. The information displayed on the display unit 21 can be read from below the container body 10.

  Further, at least one of the first sealing portion 22a and the second sealing portion 22b may have transparency that transmits a laser used for printing on a laser printing layer constituting the display portion 21. For example, when the container body 10 has transparency that transmits the laser, at least if the second sealing portion 22b joined to the container body 10 has the same transparency as the container body 10, Printing can be performed on the laser printing layer constituting the display unit 21 from above the container body 10 through the bottom of the container body 10. When the container main body 10 does not have the transparency for transmitting the laser, if the first sealing portion 22a on the opposite side to the container main body 10 has the transparency for transmitting the laser, the container Printing can be performed on the laser printing layer constituting the display unit 21 from below the main body 10. That is, it is only necessary that at least one of the container body 10 and the sealing member 22 has transparency for transmitting laser.

For example, when a YAG laser is used, a transparent resin material that transmits visible light is preferable as a resin material that transmits laser light. For example, PMMA, PC, PS, PET, PP, PE, PEN , COP, COC, etc. can be used. In addition, for example, when a CO ₂ laser is used, a polyolefin resin material such as PP, PE, COP, or COC can be used as the resin material having transparency transmitting the laser, and PE is particularly preferable. In addition, PS is preferable because it has many achievements as a material for various culture vessels and can produce a culture vessel with high safety.

In addition, from the viewpoint of facilitating joining with the container body 10 made of PS, the second sealing portion 22b joined to the container body 10 is made of PS, and the first sealing portion 22a is made of a transparent material that transmits the laser. It is preferable to use a resin material having properties. In this case, from the viewpoint of facilitating the use of various lasers, the above-mentioned polyolefin-based resin material that transmits a CO ₂ laser is used as the resin material that transmits the laser and that constitutes the first sealing portion 22a. It is more preferable to use. Further, in this case, the first sealing portion 22a that transmits the CO ₂ laser is more preferably made of PE or PP from the viewpoint of making the printing with the CO ₂ laser clearer. Further, in this case, from the viewpoint of facilitating printing and dry lamination, the first sealing portion 22a is more preferably made of PP. PE films are soft and difficult to print or dry laminate, whereas PP films are easy to handle.

  The first sealing portion 22a and the second sealing portion 22b are joined to each other around the display unit 21 to seal the display unit 21. More specifically, a sealing portion 22c is formed between the first sealing portion 22a and the second sealing portion 22b around the display portion 21, and the sealing is sealed inside the bonding portion 22c from the external environment. A region R1 is formed. The display unit 21 is sealed from the external environment by being arranged in the sealing region R1 of the sealing member 22. That is, at the peripheral portion of the first sealing portion 22a and the second sealing portion 22b, the display portion 21 is not disposed between the first sealing portion 22a and the second sealing portion 22b, and the first sealing portion 22a and the second sealing portion 22b are not arranged. Only the stop portion 22a and the second sealing portion 22b are stacked. The first sealing portion 22a and the second sealing portion 22b are closely bonded to each other at the peripheral portion, and hermetically seal and seal the display portion 21 in a sealing region R1 on the inner side.

  In the display member 20 of the present embodiment, the first sealing portion 22a and the second sealing portion 22b are joined via a joining portion 22c by, for example, thermal welding. That is, the first sealing portion 22a and the second sealing portion 22b are formed integrally by forming at least a peripheral portion thereof from a thermoplastic resin material and solidifying them by closely contacting them in a plasticized state. It is joined via the joined part 22c. Here, the bonding portion 22c between the first sealing portion 22a and the second sealing portion 22b is formed by solidifying the first sealing portion 22a and the second sealing portion 22b in a plasticized or molten state. And is a portion formed integrally with the first sealing portion 22a and the second sealing portion 22b.

  More specifically, the peripheral portion of the first sealing portion 22a and the peripheral portion of the second sealing portion 22b made of a material mainly composed of a thermoplastic resin material are brought into close contact with each other while being heated to a predetermined temperature, Cooling is performed with both molecules entering between the thermally deformed molecules. Thereby, fusion is established by the intermolecular frictional force between the first sealing portion 22a and the second sealing portion 22b, and these are formed between the first sealing portion 22a and the second sealing portion 22b. An integrated joint 22c is formed. In addition, when the heating temperature of the peripheral portion of the first sealing portion 22a and the peripheral portion of the second sealing portion 22b is much higher than the melting temperature, the peripheral portion of the first sealing portion 22a is The peripheral portion of the second sealing portion 22b is liquefied and flows by the pressing pressure. At this time, if the pressing pressure is sufficiently high, the peripheral portion of the first sealing portion 22a and the peripheral portion of the second sealing portion 22b are in a mixed state. In any case, the first sealing portion 22a and the second sealing portion 22b are made of a thermoplastic resin material, and the first sealing portion 22a and the second sealing portion 22b are plasticized by heat. It is joined via a joint 22c integrated by cooling and solidifying.

  The first sealing portion 22a and the second sealing portion 22b of the present embodiment are entirely made of a thermoplastic resin material. The resin material having thermoplasticity is not particularly limited. For example, the above-described resin materials such as PE and PS can be used. The joining portion 22c between the first sealing portion 22a and the second sealing portion 22b is formed around the display portion 21, and the display portion 21 is provided inside the display portion 21 with the first sealing portion 22a and the second sealing portion 22b. A sealing region R1 for hermetically sealing is formed therebetween.

  In addition, the display member 20 is arranged at a position that does not overlap with the observation unit 12 of the container main body 10 in plan view when the container main body 10 is viewed from above. The display member 20 is attached to the culture vessel 1 by, for example, being joined to the vessel body 10 by heat welding, or attached to the culture vessel 1 via an adhesive layer formed between the display member 20 and the vessel body 10. . When the display member 20 is joined to the container body 10 by heat welding, for example, both are made of a thermoplastic resin, and at least the joining region R2 of the peripheral portion of the display member 20 and a part of the container body 10 are brought into close contact with each other. And heat to plasticize and solidify again. As a result, a joint 30 integrated therewith is formed between the container body 10 and the display member 20, and the container body 10 and the display member 20 can be joined via the joint 30.

  When the display member 20 is joined to the container body 10 via the adhesive layer, for example, an adhesive is applied to at least one of the adhesive interfaces between the display member 20 and the container body 10 and bonded, and the adhesive is sufficiently applied. By drying and solidifying, an adhesive layer can be formed between the display member 20 and the container body 10. As the adhesive, for example, a urethane-based, imine-based, olefin-based material, or the like, which is also used as an anchor coating agent, or an ester-based or ether-based material for dry lamination can be used. The thickness of the adhesive layer can be, for example, 0.01 μm or more and 5 μm or less.

  When writing information on the display unit 21 of the display member 20, for example, the laser L is irradiated from below the container body 10 and the laser L transmitted through the sealing member 22 is irradiated on the display unit 21. For example, by moving the region where the laser L is irradiated on the display unit 21 in accordance with the information displayed on the display unit 21, the laser print layer constituting the display unit 21 changes color, and the information is displayed on the display unit 21. Is displayed. When printing with the laser L is performed on the display unit 21 before attaching the display unit 21 to the container main body 10, any one of the first sealing unit 22a and the second sealing unit 22b that transmits the laser L is used. What is necessary is just to let the laser L pass through and print on one side.

  The information displayed on the display unit 21 is usually read from above the container body 10. Therefore, when writing information on the display unit 21 by irradiating the laser L from below the container body 10 to the display member 20 attached to the bottom of the container body 10, when viewed from below the container body 10, The information to be displayed on the display unit 21 is written with the right and left reversed as in a mirror image. Thereby, the information displayed on the display unit 21 can be correctly read from above the container body 10.

  As shown in FIG. 1C, the lid member 40 is disposed on the container main body 10 so as to cover the opening 11 and closes the opening 11 of the container main body 10. A minute gap is formed between the lid member 40 and the container body 10. Thereby, the lid member 40 has air permeability between the inside and the outside of the container body 10 in a state where the opening 11 of the container body 10 is closed. The lid member 40 can be made of, for example, the same material as the container body 10.

  Hereinafter, the operation of the culture container 1 and the display member 20 of the present embodiment will be described.

  For example, when culturing cells collected from a patient such as a fertilized egg, one cell is arranged in each of a plurality of wells 14 provided in the observation unit 12 of the container main body 10 and cultured inside the partition 13. The liquid droplets are dropped and held, and the cells are cultured in a state where the container 15 is filled with oil so as to cover the periphery. The upper part of the container body 10 is opened to the outside of the container body 10 by the opening 11. In addition, the opening 11 of the container body 10 may be closed by the cover member 40, but the cover member 40 has air permeability between the inside and the outside of the container body 10. Therefore, for example, the identification information and other information of the patient are written or printed on the outer peripheral surface or the bottom surface of the container body 10, or on the outer peripheral surface or the upper surface of the lid member 40 by using an ink containing an organic solvent. In this case, the contents of the container body 10, for example, oil, culture solution, cells, and the like may be affected by components volatilized from the ink.

  On the other hand, the culture container 1 and the display member 20 of the present embodiment include a display unit 21 formed of a laser printing layer that can be printed by the laser L, and a sealing member 22 that seals the display unit 21. . Therefore, volatile components from the display unit 21, components generated by printing with the laser L, and the like are sealed by the sealing member 22, and the contents of the container body 10 are affected by the volatile components from the display unit 21. Can be prevented. In addition, since at least a part of the sealing member 22 has transparency to read information on the display unit 21, reading of information on the display unit 21 is not hindered by the sealing member 22.

  In addition, by covering the display unit 21 with the sealing member 22, the information displayed on the display unit 21 cannot be deleted or added by a method other than laser irradiation. Tampering can be prevented. This makes it possible to prevent replacement of the culture target in the culture container 1 and the like.

  In addition, by covering the display unit 21 with the sealing member 22, the information displayed on the display unit 21 is prevented from being lost or lost due to a solvent or abrasion. More specifically, for example, when the surface of the display member 20 is sterilized and washed with alcohol or the like, the information displayed on the display unit 21 is prevented from being lost or lost. Thereby, the risk of losing the information displayed on the display unit 21 can be reduced.

  The sealing member 22 includes a first sealing portion 22a and a second sealing portion 22b, and the display portion 21 is disposed between the first sealing portion 22a and the second sealing portion 22b. I have. The first sealing portion 22a and the second sealing portion 22b are joined to each other around the display unit 21 to seal the display unit 21. Thereby, for example, the sealing region R1 of the first sealing portion 22a is pattern-coated with laser printing ink to form the display portion 21, and then the first sealing portion 22a is formed around the display portion 21 around the display portion 21. By joining the two sealing portions 22b, the display portion 21 can be sealed in the sealing region R1 between the first sealing portion 22a and the second sealing portion 22b.

  Further, at least one of the first sealing portion 22a and the second sealing portion 22b has transparency for transmitting the laser L. Therefore, it is not necessary to write information in the display unit 21 before the display unit 21 is sealed by the sealing member 22. Therefore, the user of the culture container 1 and the display member 20 can freely enter necessary information on the display unit 21 when using the culture container 1 using a simple laser printing device. Therefore, responsiveness and convenience are improved as compared with a case where information is previously written or printed on the display unit 21 before the display unit 21 is sealed.

  In addition, at least one of the first sealing portion 22a and the second sealing portion 22b has transparency so that information displayed on the display portion 21 can be read. Therefore, not only is reading of the display unit 21 not hindered, but also if one of the first sealing unit 22a and the second sealing unit 22b is made of an opaque material that does not transmit visible light, The displayed information can be easily read. In this case, from the viewpoint of facilitating reading, the color of the information displayed on the display unit 21 and the color of the first sealing portion 22a or the second sealing portion 22b made of an opaque material have a predetermined contrast. Is preferred.

  More specifically, for example, when the display unit 21 has a color such as white, the display unit 21 is discolored to black by irradiating the display unit 21 with a laser, and the printed information and the display unit 21 are displayed. And a contrast between white and black can be obtained. Alternatively, the display unit 21 may be erased to obtain a transparent and white contrast between the printed information and the display unit 21. When the color of the display unit 21 is light or transparent, the first sealing unit 22a or the second sealing unit 22b that does not transmit laser when printing on the display unit 21 is made white or the like. By coloring, an appropriate contrast can be obtained between the information printed on the display unit 21 and the colored first sealing portion 22a or the second sealing portion 22b. The contrast between the two colors is preferably white and black, blue and black, white and blue, yellow and black, etc., because the information is easy to read. When the display unit 21 is irradiated with a laser to change its color, it generally changes to black. Therefore, the surrounding area is preferably white or blue.

  The first sealing portion 22a and the second sealing portion 22b are made of a thermoplastic resin material, and are joined via a joining portion 22c integrated with the first sealing portion 22a and the second sealing portion 22b. Have been. Therefore, the first sealing portion 22a and the second sealing portion 22b can be directly joined without using an adhesive or an adhesive. Therefore, no volatile component is generated from the joint portion 22c between the first sealing portion 22a and the second sealing portion 22b, and the content of the container body 10 is not affected by the volatile component from the adhesive or the adhesive. Can be prevented.

  In addition, the culture container 1 of the present embodiment is usually shipped with the display member 20 attached to the container body 10 in advance, but the culture container 1 without the display member 20, that is, the container body 10, May be shipped individually. Further, the culture container 1 may be shipped as a culture container kit including a container body 10 and a display member 20 attached to the container body 10. In these cases, a user is provided with a heat welding device for heat-sealing the display member 20 to the container body 10 and an adhesive for bonding the display member 20 to the container body 10.

  When the display member 20 is attached to the container body 10 by heat welding, it is not necessary to use an adhesive or an adhesive, so that no volatile component is generated from the joint 30 between the display member 20 and the container body 10. It is possible to prevent the contents of the container body 10 from being affected by volatile components from the adhesive or the adhesive. When the display member 20 is attached to the container main body 10 with an adhesive, the adhesive is sufficiently dried and solidified to form an adhesive layer in which volatile components are sufficiently volatilized in advance, so that the container main body 10 The contents can be prevented from being affected by the volatile components from the adhesive layer. Note that by using a non-solvent-based adhesive that is cured by a chemical reaction such as a silicone-based or epoxy-based adhesive, it is also possible to fix without using a volatile component.

  On the other hand, when the display member 20 is attached to the container main body 10 via an adhesive or an adhesive layer that maintains the adhesiveness even after the adhesion, the contents of the container main body 10 are removed by the volatile components from the adhesive or the adhesive layer. May be affected. The pressure-sensitive adhesive or pressure-sensitive adhesive layer has properties of both a solid and a liquid, and can stably maintain a wet and flexible state by containing a solvent, and can be adhered and peeled off. There is no significant change in the state.

  The display member 20 is attached to the outer bottom surface of the container body 10. Thereby, the possibility that the contents of the container body 10 are contaminated by, for example, various bacteria or endotoxin can be reduced. When the display member 20 is attached to the inner bottom surface of the container body 10, if the display member 20 is sufficiently sterilized and washed to ensure hygiene and prevent contamination, the outer bottom surface of the container body 10 is prevented. Can be made flat and thin, and observation with a microscope can be facilitated. In addition, when the display member 20 is attached to the outer or inner side surface of the container body 10, the display member 20 can be prevented from obstructing observation by a microscope.

  As described above, according to the culture container 1, the display member 20, and the culture container kit of the present embodiment, the display unit 21 that displays information on the container main body 10 has transparency that allows the information of the display unit 21 to be read. Sealed by a sealing member 22 having the same. Accordingly, reading of the display unit 21 is not hindered by the sealing member 22, and components volatilized from the display unit 21 are sealed by the sealing member 22, thereby preventing the contents of the container body 10 from being affected. It is not necessary to use an adhesive layer containing a volatile component to attach the display member 20 to the container, and it is possible to prevent the contents of the container body 10 from being affected by the volatile component from the adhesive layer.

  In the present embodiment, an example in which the sealing member 22 includes the first sealing portion 22a and the second sealing portion 22b has been described. However, as long as at least a part of the sealing member 22 is a member that seals the display unit 21 with transparency so that the information printed on the display unit 21 can be read, the first sealing unit 22a and the second sealing unit 22a are used. The two sealing portions 22b may not be provided. For example, the sealing member 22 may be a resin layer that coats the surface of the display unit 21. Further, the display section 21 may be formed so as to be embedded in a sealing member 22 made of a resin material.

  In the present embodiment, the configuration in which the display member 20 is attached to the container body 10 has been described. However, as shown in FIG. 1C, the display member 20 may be attached to the lid member 40. In this case, the culture vessel 1 includes a container body 10 having an open opening 11, a cover member 40 for closing the opening 11 of the container body 10, and a display unit 21 for displaying information on the cover member 40. And a sealing member 22 for sealing the display unit 21. The display member 20 may be attached to both the container body 10 and the lid member 40, or may be attached to either one of them.

[Embodiment 2]
3A and 3B are cross-sectional views of a display member 20A according to Embodiment 2 of the present invention.

  The display member 20A of the present embodiment differs from the display member 20 of the first embodiment shown in FIG. 2 in that the first sealing portion 22a and the second sealing portion 22b are joined via an adhesive layer 22d. Is different. The other points of the display member 20A of the present embodiment are the same as those of the display member 20 of the first embodiment, and thus the same portions are denoted by the same reference numerals and description thereof will be omitted.

  In the display member 20A of the present embodiment, the first sealing portion 22a and the second sealing portion 22b are connected via the adhesive layer 22d solidified between the first sealing portion 22a and the second sealing portion 22b. Are joined. That is, the first sealing portion 22a and the second sealing portion 22b of the sealing member 22A are joined by, for example, dry lamination, and an extremely thin portion is provided between the first sealing portion 22a and the second sealing portion 22b. An adhesive layer 22d is formed. More specifically, the first sealing portion 22a and the second sealing portion 22b, which are resin materials, are bonded via a very small amount of adhesive, and then are sufficiently dried and solidified by, for example, a drying device. Thus, the first sealing portion 22a and the second sealing portion 22b are joined via the adhesive layer 22d. This can prevent volatile components from remaining on the adhesive layer 22d.

  As the adhesive, for example, a urethane-based, imine-based, olefin-based material, or the like, which is also used as an anchor coating agent, or an ester-based or ether-based material for dry lamination can be used. The thickness of the adhesive layer 22d after drying can be, for example, 0.01 μm or more and 5 μm or less. By setting the thickness of the adhesive layer 22d in such a range, a sufficient bonding strength can be obtained while sufficiently suppressing volatile components from the adhesive layer 22d. Therefore, according to the display member 20A of the present embodiment, similarly to the display member 20 of the first embodiment, it is not necessary to use an adhesive layer for joining the first sealing portion 22a and the second sealing portion 22b, and The same effect as that of the display member 20 according to the first embodiment can be obtained. Note that, unlike the adhesive layer 22d, the adhesive layer does not usually solidify, and maintains adhesiveness even after bonding. Therefore, when an adhesive layer containing a volatile component such as an organic solvent is used, the organic solvent and the like volatilize even after bonding.

  Note that the thickness of the adhesive layer 22d is preferably 2 μm or less, and more preferably 1 μm or less. This is because, as the thickness of the adhesive layer 22d is smaller, the risk of residual solvent can be reduced when the adhesive layer 22d contains an organic solvent. In addition, by forming the adhesive layer 22d using a non-solvent adhesive that is cured by a chemical reaction such as a silicone-based or epoxy-based adhesive, the adhesive layer 22d containing no volatile component can be formed. Also, the adhesive used for dry lamination, without using an organic solvent, by using an insoluble adhesive that can be adjusted to an appropriate viscosity by heating, it is also possible to form an adhesive layer 22d containing no volatile components. It is possible.

  Further, the first sealing portion 22a and the second sealing portion 22b may be made of the same material or different materials. Also, as shown in FIG. 3A, both the first sealing portion 22a and the second sealing portion 22b may have the same transparency as the sealing member 22 of the first embodiment. As shown in (1), for example, the first sealing portion 22a may not have transparency. As described above, even when the first sealing portion 22a and the second sealing portion 22b are made of different materials, the first sealing portion 22a and the second sealing portion 22d are formed by the adhesive layer 22d formed by dry lamination. 22b can be securely and firmly joined, and the sealing property of the display unit 21 can be ensured by the sealing member 22A.

  In addition, the display member 20A of the present embodiment has the display portion 21 formed of a laser printing layer that can be printed with a laser, and at least one of the first sealing portion 22a and the second sealing portion 22b, for example, the second sealing portion. 22b has transparency which transmits a laser. Therefore, it is possible to irradiate the display unit 21 with the laser through the second sealing unit 22b that transmits the laser L. Therefore, according to the display member 20A of the present embodiment, the same effect as the display member 20 of the first embodiment can be obtained even when the first sealing portion 22a does not have transparency.

[Embodiment 3]
FIG. 4 is a cross-sectional view of a display member 20B according to Embodiment 3 of the present invention.

  The display member 20B of the present embodiment is different from the display member 20A of the above-described second embodiment in that an ink that changes color by laser irradiation is not used as a laser printing layer constituting the display unit 21B. The other points of the display member 20B of the present embodiment are the same as those of the display member 20A of the second embodiment, and thus the same portions are denoted by the same reference numerals and description thereof will be omitted.

  The display portion 21B of the display member 20B of the present embodiment includes a first layer 21a that causes portions corresponding to characters to be printed to disappear by laser irradiation, a second layer 21b that is exposed at a portion where the first layer 21a has disappeared, It has. The first layer 21a is made of, for example, a material that absorbs laser energy, and the second layer 21b is made of a material that does not disappear when the first layer 21a is irradiated with a laser and disappears. . Further, the first layer 21a and the second layer 21b have distinctly different colors, for example, black and white. As described above, when the display unit 21B has a two-layer structure of the first layer 21a and the second layer 21b, the second layer 21b exposed at a portion where information is printed and the first layer 21a remaining around the second layer 21b are formed. And a good contrast can be obtained. For example, the first layer 21a to be erased by laser can be black, yellow, blue, or the like, and the second layer 21b exposed by laser irradiation can be white.

  When writing information on the display section 21B, the display section 21B is irradiated with laser through the second sealing section 22b of the sealing member 22A, and for example, the information is displayed according to the information displayed on the display section 21B. The area to be irradiated with the laser L is moved to the portion 21B. Accordingly, the first layer 21a of the display unit 21B disappears and the second layer 21b is exposed, and information is displayed on the display unit 21B. Therefore, according to the display member 20B of the present embodiment, the same effect as the display members 20 and 20A of the first and second embodiments when the ink that changes color by laser irradiation is used as the laser printing layer constituting the display portion 21B. Can be obtained.

[Embodiment 4]
Next, a fourth embodiment of the present invention will be described with reference to FIGS. 1A and 1B and FIG. FIG. 5 is a cross-sectional view of the vicinity of the display unit 21 corresponding to FIG. 1C of the culture vessel 1A according to Embodiment 4 of the present invention.

  The culture container 1A of the present embodiment differs from the culture container 1 of the first embodiment in that the sealing member 22B seals the display unit 21 together with the container body 10. Other points of the culture vessel 1A of the present embodiment are the same as those of the culture vessel 1 of the first embodiment, and therefore, the same portions are denoted by the same reference numerals and description thereof will be omitted.

  The culture container 1A of the present embodiment includes a container body 10 having an open opening 11, a display unit 21 for displaying information on the container body 10, and a sealing member 22B for sealing the display unit 21. ing. The display unit 21 is disposed between the container main body 10 and the sealing member 22B. The sealing member 22B is joined to the container main body 10 and is between the container main body 10 and the sealing member 22B around the display unit 21. The display unit 21 is sealed.

  When the container body 10 and the sealing member 22B are made of a thermoplastic resin material as in the first embodiment, after the display unit 21 is disposed in the sealing region R1 of the sealing member 22B, heat sealing is performed. Thereby, the sealing member 22B can be joined to the container body 10. More specifically, in the joining region R2 outside the display unit 21, the container body 10 and the sealing member 22B are plasticized by heat and solidified in a state of being in close contact with each other. By forming the joint portion 30 integrated with the container body 10 and the sealing member 22B, the sealing member 22B can be joined to the container body 10. Thereby, the sealing region R1 is sealed, and the display unit 21 is sealed between the culture container 1A and the container main body 10.

  According to the culture container 1A of the present embodiment, the display unit 21 for displaying information on the container body 10 is sealed by the sealing member 22B. Therefore, the same effect as the culture container 1 of the first embodiment can be obtained.

  The sealing member 22B may be joined to the container body 10 via an adhesive layer, similarly to the display member 20 of the first embodiment. Further, the position at which the sealing member 22B is joined to the container body 10 is not particularly limited as in the case of the display member 20 of the first embodiment. The display unit 21 may be, for example, a thin plate or sheet member in which the above-described information is previously written or printed with normal ink before sealing with the sealing member 22B. Also in this case, the volatile component from the display unit 21 is sealed by the sealing member 22B, and the contents of the container body 10 can be prevented from being affected by the volatile component from the display unit 21. However, since information can be written by the laser L after the sealing of the display unit 21, it is preferable that the display unit 21 be formed of a laser printing layer as in the first embodiment.

  Alternatively, the sealing member 22B may be part of the container body 10, the sealing member 22B may be provided integrally with the container body 10 by insert molding, and the display unit 21 may be embedded in the container body 10 for sealing. . As described above, by integrally providing the sealing member 22B and the container body 10 by insert molding, it is not necessary to use an adhesive layer, and an effect equivalent to that of the above-described culture container 1A can be obtained. Even when the sealing member 22B and the container main body 10 are not integrally provided by insert molding, the bottom of the container main body 10 is flattened by providing a concave portion for accommodating the display member 20 at the bottom of the container main body 10. be able to.

  In the present embodiment, the configuration in which the sealing member 22B seals the display unit 21 together with the container body 10 has been described. However, when the display unit 21 is provided on the lid member 40, the sealing member 22B is The display unit 21 may be sealed together with the member 40. That is, as shown in FIG. 1C, instead of attaching the display member 20 to the lid member 40 that closes the opening 11 of the container body 10, the display unit 21 is disposed between the lid member 40 and the sealing member 22, The display unit 21 may be sealed between the lid member 40 and the sealing member 22 by joining the sealing member 22 to the lid member 40.

[Embodiment 5]
FIG. 6A is a plan view at the time of assembling the culture container kit 100 according to Embodiment 5 of the present invention. FIG. 6B is a cross-sectional view of the culture container kit 100 along the line BB shown in FIG. 6A.

  The culture container kit 100 of the present embodiment is different from the culture container 1 of the first embodiment in that the display member 20D is fixed by being detachably engaged with the fixing portion 16 of the container main body 10A. Other points of the culture container kit 100 of the present embodiment are the same as those of the culture container 1 of the first embodiment, and therefore, the same portions are denoted by the same reference numerals and description thereof will be omitted.

  The culture container kit 100 of the present embodiment includes a container main body 10A having an open opening 11 and a display member 20D attached to the container main body 10A, similarly to the container main body 10 of the culture container 1 of the first embodiment. In addition, as shown in FIG. 6B, the container body 10A has a fixing portion 16 for fixing the display member 20D.

  Similar to the display member 20 of the first embodiment, the display member 20D of the present embodiment has a display unit 21 for displaying information on the container main body 10A, and at least a part having transparency capable of reading the information of the display unit 21. And a sealing member 22 for sealing the display unit 21. As shown in FIG. 6A, the display member 20D is arranged on the bottom of the container body 10A in a region other than the observation unit 12 where the specimen is arranged, and corresponds to the observation unit 12 in plan view when viewed from above the container body 10A. It has an opening 23 at a position and is formed in an annular shape.

  The fixing portion 16 of the container body 10A has an engaging portion 17 that extends downward from the bottom surface of the container body 10A and is bent in a direction along the bottom surface of the container body 10A. The engaging portions 17 may be provided continuously along the circumferential direction of the container main body 10A which is circular in plan view, and a plurality of engaging portions 17 are provided along the circumferential direction of the container main body 10A. They may be arranged at intervals along the circumferential direction.

  When fixing the display member 20D to the fixing portion 16 of the container main body 10A, for example, the engaging portion 17 on the radially inner side of the container main body 10A is elastically bent so that the opening 23 of the display member 20D is engaged with the inner side. The outer edge of the display member 20D is engaged with the outer engagement portion 17 by elastically bending the outer engagement portion 17 of the display member 20D or the container body 10A in the radial direction. . As a result, the inner edge and the outer edge of the annular display member 20D are engaged with the engagement portions 17, 17, and the display member 20D is fixed to the fixing portion 16 of the container body 10A.

  According to the culture container kit 100 of the present embodiment, as in the case of the display member 20 of the first embodiment, the display member 20 </ b> D has the transparency that can read the information of the display unit 21 and seals the display unit 21. Since the stop member 22 is provided, the same effects as those of the culture vessel 1 and the display member 20 of the first embodiment can be obtained at the time of assembly. In addition, since an adhesive layer is not required to fix the display member 20D to the container body 10A, it is possible to prevent the contents of the container body 10A from being affected by volatile components from the adhesive layer.

  According to the culture container kit 100 of the present embodiment, the display member 20D does not need to be thermally welded or bonded to the container main body 10A, and the display member 20D can be easily fixed to the container main body 10A. It can be attached and detached. In addition, since the display member 20D has the opening 23 at a position corresponding to the observation unit 12, it is possible to prevent the display member 20D from obstructing observation of cells and the like.

  In the present embodiment, the configuration in which the container body 10A has the fixing portion 16 has been described, but the fixing portion 16 may be provided on the lid member 40. Further, the display member 20D is not limited to the one having the opening 23. The display member 20D may be any as long as it does not overlap the observation unit 12 in a plan view when viewed from above the container body 10A. The display member 20D can adopt any shape such as an arc shape, a U shape, a channel shape, and a strip shape that do not overlap the observation unit 12, for example. In addition, if the display member 20D including the display unit 21 and the sealing member 22 has transparency that transmits visible light and does not hinder observation of the observation unit 12 by a microscope or the like, the display member 20D May overlap. It should be noted that the annular display member 20D having the opening 23 allows the display member 20D to be designed larger at the time of fixing and working as compared with other shapes, and the workability is improved.

[Embodiment 6]
FIG. 7A is a plan view of a culture vessel 1A according to Embodiment 6 of the present invention. FIG. 7B is a sectional view taken along line BB shown in FIG. 7A.

  The culture container 1B of the present embodiment is such that the display unit 21A is formed inside the container main body 10, that is, in the material constituting the container main body 10, and the container main body 10 around the display unit 21A functions as a sealing member 22C. This is different from the culture container 1 of the fourth embodiment. The other points of the present embodiment are the same as those of the culture vessel 1 of the fourth embodiment, and therefore, the same portions are denoted by the same reference characters and description thereof is omitted.

  The culture container 1B of the present embodiment is provided integrally with the container main body 10 having an open opening 11, a display portion 21A for displaying information on the container main body 10, and the container main body 10, and is capable of reading information. A sealing member 22C for sealing the display section 21A with transparency.

  The container main body 10 of the culture container 1B of the present embodiment is made of, for example, glass having transparency from which information on the display unit 21A can be read. The display unit 21A according to the present embodiment, for example, focuses a laser on the inside of the container body 10 to generate a minute crack to make it opaque, so that the information displayed on the container body 10 can be displayed inside the container body 10. This is the part directly written in. In the present embodiment, the container body 10 around the display unit 21A functions as a sealing member 22C that seals the display unit 21A.

  In the culture container 1B of the present embodiment, the display unit 21A that displays information on the container body 10 is sealed with a transparent sealing member 22C that can read the information of the display unit 21A, and the adhesive layer containing a volatile component. There is no need to use Therefore, according to the culture vessel 1B of the present embodiment, not only the same effects as those of the culture vessel 1 of Embodiment 1 can be obtained, but also the surface has no irregularities and the sanitary property can be improved. In the culture container 1B of the present embodiment, the material of the container body 10 is not limited to glass as long as it can be made opaque by focusing a laser on the inside of the container body 10.

  As described above, the embodiment of the present invention has been described in detail with reference to the drawings. However, the specific configuration is not limited to this embodiment, and there are design changes and the like without departing from the gist of the present invention. They are also included in the present invention.

[Examples and Comparative Examples]
Hereinafter, a display member according to an example of the present invention and a label according to a comparative example different from the present invention will be described.

(Example 1)
Based on the display member 20A of Embodiment 2 shown in FIG. 3, the first sealing portion 22a and the second sealing portion 22b are made of PS sheets, and laser printing ink (Toyo Ink) is formed on the first sealing portion 22a. A display member 20A having the display unit 21 as a laser printing layer was manufactured by pattern-coating a gravure ink manufactured by Elbima Z117). The thickness of each of the first sealing portion 22a and the second sealing portion 22b was about 40 μm, and the size was 10 mm long × 20 mm wide. The width of the joint 22c formed around the display unit 21 was 10 mm.

(Example 2)
Based on Embodiment 2 described above, the first sealing portion 22a shown in FIG. 3 is a sheet made of PS, the second sealing portion 22b is a sheet made of PE, and the laser is placed on the first sealing portion 22a. By pattern-coating a printing ink (for printing with a CO ₂ laser), a display member 20A having the display unit 21 as a laser printing layer was manufactured. The thickness of the first sealing part 22a is about 40 μm, the thickness of the second sealing part 22b is about 50 μm, and the size of the first sealing part 22a and the second sealing part 22b and the size of the bonding part 22c The width was the same as in Example 1.

(Example 3)
Based on Embodiment 2 described above, the first sealing portion 22a and the second sealing portion 22b shown in FIG. 3 are made of PE sheets, and the same laser printing ink as that of Example 2 is formed on the first sealing portion 22a. By pattern coating, a display member 20A having the display portion 21 as a laser printing layer was manufactured. The thickness of the first sealing portion 22a and the second sealing portion 22b was the same as the size, and the width of the joining portion 22c was the same as in the second embodiment.

(Example 4)
Based on Embodiment 2 described above, the first sealing portion 22a shown in FIG. 3 is a sheet made of PP, the second sealing portion 22b is a sheet made of PS, and the laser is placed on the first sealing portion 22a. By pattern-coating the printing ink, a display member 20A having the display unit 21 as a laser printing layer was manufactured. The thickness of the first sealing part 22a is about 20 μm, the thickness of the second sealing part 22b is about 40 μm, and the size of the first sealing part 22a and the second sealing part 22b, the bonding part 22c Was the same as in Example 1.

(Comparative Example 1)
Printing was performed by an ink jet printer on a printing portion of a commercially available label with an adhesive layer. The material of the label was synthetic paper YUPO (registered trademark), and the size of the label was 30 mm long × 30 mm wide. The material of the adhesive layer was an acrylic material, and the thickness of the adhesive layer was about 10 μm.

(Comparative Example 2)
The patient's name and the like were written on the outer surface of the container body 10 of the culture container 1 using a commercially available felt pen.

(Evaluation 1)
The display members 20A of the first to fourth embodiments are prepared, and the display unit 21 is irradiated with a YAG laser having an output of about 5 W and a wavelength of 1064 nm from the first sealing unit 22a via the first sealing unit 22a. 21 was evaluated for printability. Next, the display members 20A of Examples 2 and 4 are prepared, and the display unit 21 is irradiated with the same YAG laser from the side of the second sealing unit 22b via the second sealing unit 22b to print on the display unit 21. The possibility was evaluated. Next, the same YAG laser was applied to the printed portion of the label of the comparative example to evaluate whether the printed portion could be printed.

(Evaluation 2)
The same evaluation as Evaluation 1 was performed using a CO ₂ laser having an output of about 10 W and a wavelength of 10.6 μm instead of the YAG laser.

(Evaluation 3)
The display members 20A of Examples 1 to 4 after printing and the label of Comparative Example 1 after printing were prepared, and these were analyzed by gas chromatography to evaluate the presence or absence of outgassing of organic components.

  The results of the above evaluations 1 to 3 are shown in Table 1 below.

From the results of Evaluation 1 and Evaluation 2, in the case of using the first sealing portion 22a or the second sealing portion 22b made of PE or PP, the display members 20A of Examples 1 to 4 use the YAG laser and CO _2. Printing was possible using any of the lasers, and there was no difference in the ease of printing depending on the type of laser. On the other hand, in the display members 20A of Examples 1 to 4, when the first sealing portion 22a or the second sealing portion 22b made of PS or PE is used, there is a tendency that printing with the CO ₂ laser tends to be difficult. Was done. That is, the CO ₂ laser tends to be easily affected by the material of the sealing member 22A, and when the PE, which is a polyolefin-based resin material, is used as the material of the sealing member 22A, the display portion 21 Printing was easy

  Also, from the measurement result of the evaluation 3, the gas output amount of the display member 20A of Examples 1 to 4 is about 200 ng or less in hexadecane conversion value, and the gas output amount of the label of Comparative Example 1 is about 2000 ng, The amount of gas emitted from the printing section by the felt-tip pen No. 2 was about 800 ng. Since the amount of gas emitted from the display member 20 in Examples 1 to 4 is extremely small compared to the amount of gas emitted from the labels in Comparative Examples 1 and 2, the amount of gas emitted from the display member 20 in Examples 1 to 4 is small. Is "none" (extremely small amount), and outgases of the labels and the felt pens of Comparative Examples 1 and 2 are "present". From the above results, it was confirmed that the display members 20A of Examples 1 to 4 prevented generation of volatile components that might affect the contents of the culture vessel 1.

1, 1A, 1B Culture container 10, 10A Container main body 11 Opening 12 Observation unit 16 Fixed unit 20, 20A-20D Display member 21, 21A Display unit 22 Sealing members 22A-22C Sealing member 22a First sealing portion 22b Second sealing portion 22c Joining portion 22d Adhesive layer 23 Opening 40 Lid member 100 Culture container kit

Claims (1)

A display member attached to the culture vessel,
A display unit including a laser printing layer that can be printed by a laser, and a sealing member that seals the display unit with at least a portion having transparency that can read information printed on the display unit, A display member characterized by the above-mentioned.

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