JP2019023597A - Orp digitalization determination device and method for using the same - Google Patents
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Abstract
Description
本発明はＯＲＰ数値化判定装置および使用方法にかんする。
The present invention relates to an ORP digitization determination apparatus and method of use.
酸化還元電位測定装置は指示電極と参照電極を備え、指示電極と参照電極を試料溶液中に挿入し、溶液中の電解質の濃度や酸化体・還元体のイオン濃度比など酸化還元電位を測定する。従来、酸化還元電位の測定は主に排水やプラント水の水質検査など多量の溶液を対象とする一方、試料溶液が少量で、緩衝能力が低い場合、測定の再現精度が不安定で正確性にかけるという欠点があった。液絡部を通して試料溶液中に流出する参照電極（銀−塩化銀）側の内部液が試料溶液と接触することによって酸化還元電位が測定されるのであるが、指示電極とする白金電極との距離が近いことにより検体液以外の参照電極（銀−塩化銀）側の内部液が試料槽部に装填される指示電極に接触してしまうことで緩衝能力の低い試料溶液（唾液などの生体液等）のような場合、参照電極の内部液の流出がわずかであっても検体溶液に対する影響が大きく、指示電極に直接接触した場合は、検体溶液の測定ではなく、内部溶液に影響された測定となり正確な酸化還元電位測定ができないからである。 The oxidation-reduction potential measuring device includes an indicator electrode and a reference electrode, and the indicator electrode and the reference electrode are inserted into the sample solution, and the oxidation-reduction potential such as the concentration of the electrolyte in the solution and the ion concentration ratio of the oxidant / reductant is measured. . Conventionally, redox potential measurement is mainly for large quantities of solutions such as water quality inspection of wastewater and plant water. On the other hand, if the sample solution is small and the buffer capacity is low, the reproducibility of measurement is unstable and accurate. There was a drawback of calling. The redox potential is measured when the internal solution on the side of the reference electrode (silver-silver chloride) flowing out into the sample solution through the liquid junction comes into contact with the sample solution, but the distance from the platinum electrode as the indicator electrode Sample solution with low buffering capacity (biological fluid such as saliva etc.) due to the internal solution on the reference electrode (silver-silver chloride) side other than the sample solution coming into contact with the indicator electrode loaded in the sample tank ), Even if the internal solution of the reference electrode is small, it has a large effect on the sample solution. If it is in direct contact with the indicator electrode, the measurement is influenced by the internal solution, not the sample solution. This is because an accurate redox potential cannot be measured.
特許文献１は、測定電極及び比較電極を被検液に浸漬して被検液の酸化還元電位を測定する酸化還元電位測定装置の電極を検査する方法、及びその検査に使用する標準液に関するものを提供しているものである。
Patent Document 1 relates to a method for inspecting an electrode of an oxidation-reduction potential measuring device for measuring the oxidation-reduction potential of a test solution by immersing a measurement electrode and a comparison electrode in the test solution, and a standard solution used for the inspection It is something that provides.
特許文献２は、過硫酸塩を活性炭により還元処理する過硫酸塩処理装置、過硫酸塩処理方法の技術に関するものである。
Patent Document 2 relates to a technique for a persulfate treatment apparatus and a persulfate treatment method for reducing persulfate with activated carbon.
特許文献３及び特許文献４とも本出願人の発明による人間の生体液である唾液の酸化還元電位を測定する装置について提供している。 Patent Documents 3 and 4 both provide an apparatus for measuring the redox potential of saliva, which is a human biological fluid, according to the applicant's invention.
本発明は、特許文献３及び特許文献４で示す通り、酸化還元電位測定装置は指示電極と参照電極を備え、指示電極と参照電極を試料溶液中に挿入し、溶液中の電解質の濃度や酸化体・還元体のイオン濃度比など酸化還元電位を測定する人間の唾液の酸化還元電位測定装置であるが、検体液を含浸させた綿棒を接触させる試料槽部に新しい検体液の測定ごとに綿棒に添えて丸棒形状の指示電極を装填することで酸化還元電位を測定するのであるが、測定装置本体の外付け装着の白金電極においては、測定装置使用者の取り扱いが粗雑な場合は、丸棒形状の白金電極を折り曲げ微細な傷をつける問題が生じることになり、それにより、測定精度の大きな値差が生じるのである。
In the present invention, as shown in Patent Document 3 and Patent Document 4, the oxidation-reduction potential measuring device includes an indicator electrode and a reference electrode, the indicator electrode and the reference electrode are inserted into a sample solution, and the concentration and oxidation of the electrolyte in the solution are measured. This is a device for measuring the redox potential of human saliva that measures the redox potential such as the ion concentration ratio of the body / reductant. In addition, the redox potential is measured by loading a round bar-shaped indicator electrode with a platinum electrode attached externally to the measuring instrument body. There arises a problem that the rod-shaped platinum electrode is bent and finely scratched, thereby causing a large value difference in measurement accuracy.
従来においては、ORP電極の性能チェックに用いる校正標準溶液においては、酸化体と還元体が共存しない、溶存酸素（DO）、すなわち水質に含む酸素の量が極めて少ないキンシドロン液など、空気に触れても酸化側にも還元側にも傾かない比較的安定しているとしてキンシドロン溶液を用いてORP電極の性能チェックを行うための標準溶液が使用されている。
Conventionally, calibration standard solutions used to check the performance of ORP electrodes are exposed to air, such as quincidrone solution in which the oxidant and reductant do not coexist, dissolved oxygen (DO), that is, the amount of oxygen contained in the water is extremely small. A standard solution is used to check the performance of the ORP electrode using a quincidron solution because it is relatively stable, neither inclined nor oxidized or reduced.
当発明のORP数値化判定装置および使用方法においては、測定対象試料に酸化体と還元体が混在している試料液であり、この酸化体と還元体の電子授受の電位差を計測するのであり、酸化体と還元体が共存しない、すなわち、溶存酸素（DO）が存在しない溶液を用いてORP電極の性能チェックでは、本発明のORP数値化判定装置および使用方法の校正標準溶液の要件を果たせないと考える。
In the ORP digitization determination apparatus and method of use of the present invention, it is a sample liquid in which an oxidant and a reductant are mixed in a sample to be measured, and the potential difference in electron transfer between the oxidant and the reductant is measured. In the ORP electrode performance check using a solution in which the oxidant and the reductant do not coexist, that is, there is no dissolved oxygen (DO), the requirement of the calibration standard solution of the ORP digitization judgment device and the method of use of the present invention cannot be fulfilled. I think.
この課題を解決するため本発明にかかる酸化還元電位測定装置は、検体液である人間の唾液採取の綿棒、又は各種水溶液を含浸させた綿棒を参照電極と指示電極に介在させることで、溶液中の電解質の濃度や酸化体・還元体のイオン濃度比など酸化還元電位を測定する構成としている。 In order to solve this problem, the oxidation-reduction potential measuring device according to the present invention includes a swab for collecting human saliva, which is a sample solution, or a cotton swab impregnated with various aqueous solutions, interposed between a reference electrode and an indicator electrode. It is configured to measure the redox potential such as the concentration of the electrolyte and the ion concentration ratio of the oxidant / reductant.
図１で示す通り、基準電極、銀―塩化銀電極KCｌ １mo（KCl 内部液濃度は限定されない）が接触する液絡部と試料槽部の底に内蔵された指示電極の三者間が常に一定の距離を設けられ、測定中に指示電極が溶液含浸綿棒に覆われることで空気に触れて起こる酸化還元の緩衝を受けずに、測定対象試料とする溶液含浸のみの酸化還元電位ORP値（ｍV）が安定して電子授受交換ができるように構成されている。 As shown in Fig. 1, the distance between the reference electrode and the silver-silver chloride electrode KCl 1mo (KCl internal liquid concentration is not limited) and the indicator electrode built in the bottom of the sample tank are always constant. The measurement electrode is covered with a solution-impregnated cotton swab during the measurement, so that the oxidation-reduction potential ORP value (mV ) Can be exchanged electronically stably.
つぎに、図2で示す通り、基準電極、銀―塩化銀電極KCｌ １mol（KCl 内部液濃度は限定されない）を格納する１５ 内部液を格納する液槽の上部蓋となるうえ下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ１molの溶液を介して連通させるようにしたことにより、指示電極は空気に触れて酸化影響を受けることなくKCl溶液を通してプラスマイナスの電子導通が加速されることの証としての表1、表2のデータによって後述する。 Next, as shown in Fig. 2, the reference electrode, silver-silver chloride electrode KCl 1mol (KCl internal liquid concentration is not limited) is stored. By connecting the reference electrode and the indicator electrode through the KCl 1 mol solution, the indicator electrode is exposed to air and is not affected by oxidation. This will be described later with the data in Tables 1 and 2 as proof that conduction is accelerated.
なぜなら、浴槽の天井の水滴の動きは天井平面より、天井側に溝を掘ってある箇所ほど早い速度で流れることに注目し、肉眼では目視できない電子の流れも引力に引き付けられる状態でありながら、より移動速度は高まり電子授受が安定し、酸化還元電位測定の再現精度を高められと考察したのである。 Because the movement of water drops on the ceiling of the bathtub flows faster at the location where the groove is dug on the ceiling side than the ceiling plane, while the flow of electrons that can not be seen with the naked eye is also attracted to the attractive force, He thought that the transfer speed would be higher, the electron transfer would be stable, and the reproducibility of the redox potential measurement could be improved.
上記目的を達成するために、本発明は（請求項１の構成要件）とで、本発明の溶液中の電解質の濃度や酸化体・還元体のイオン濃度比など酸化還元電位を測定する構成であり、前記浴槽の天井の水滴の動きは天井平面より、天井側に溝を掘ってある箇所ほど早い速度で流れることに注目し、肉眼では目視できない電子の流れも引力に引き付けられる状態でありながら、より移動速度は高まり電子授受が安定し、酸化還元電位測定の再現精度を高められることを突き止め、表１に示す通り、本発明のORP数値化判定装置および使用方法の校正標準溶液の要件を果たせるようにしたのである。
In order to achieve the above object, the present invention is configured to measure an oxidation-reduction potential such as the concentration of the electrolyte in the solution of the present invention and the ion concentration ratio of the oxidant / reductant. Yes, note that the movement of water drops on the ceiling of the bathtub flows faster at the location where the groove is dug on the ceiling side than the ceiling plane, and the flow of electrons that cannot be seen with the naked eye is also attracted to the attractive force As a result, it was found that the transfer speed was increased, the electron transfer was stable, and the reproducibility of the redox potential measurement could be improved. I was able to do it.
以上の説明から明らかなように、本発明にあっては次に列挙する効果が得られる。
（１）（請求項１の構成要件）とで構成されているので、人間の唾液の酸化還元電位（ＯＲＰ）測定手段を用いて、本発明の有効性を証明するために、本発明者が取得している特許をもとに開発した厚生労働省から医療機器として認証されている人間の唾液ＯＲＰ測定装置と本発明のORP数値化判定装置および使用方法により、本発明で用いる校正標準溶液とする溶液は、KCｌ １mol溶液により測定精度の比較を試みたのである。
As is clear from the above description, the present invention has the following effects.
In order to prove the effectiveness of the present invention using the means for measuring redox potential (ORP) of human saliva, the present inventor The calibration standard solution used in the present invention is based on the human saliva ORP measurement device and the ORP quantification judgment device and method of use of the present invention that have been certified as medical devices by the Ministry of Health, Labor and Welfare developed based on the patents that have been acquired. The solution was an attempt to compare the measurement accuracy with a 1 mol KCl solution.
（２）前記（１）によって、試料槽部の底に指示電極（白金）を装填した人間の唾液の酸化還元電位（ＯＲＰ）測定手段を用いて、すでに、厚生労働省から医療機器として認証されている人間の唾液ＯＲＰ測定装置を用いて８，０００症例を超える唾液臨床でｍＶ数値限定による体調度を立証した横浜・小児内科医の岡澤美江子医師の協力のもと、患者及び付き添いの健
常者を含む２０人の同一溶液である唾液ＯＲＰの測定を実施した。表１は、ORP電極の性能チェックに用いる校正標準溶液を確立するために、酸化体と還元体が共存する溶液を校正標準溶液とするために、本発明装置に備えた各収納式の試料槽ＢＯＸ1、試料槽ＢＯＸ２、至る
試料槽ＢＯＸ３で、KCｌ １mol溶液を用いてORP測定精度の比較を試みたのである。その結
果、表1で示す通り、日時が異なる5日間、各5回の測定結果はプラス６０ｍＶを基準にプラスマイナス５ｍＶ以内の再現数値が示され、たまたま偶然に一致したとは考えられず、本発明のORP数値化判定装置および使用方法の校正標準溶液としての所期の目的を達成したのであり、また、唾液ORP測定装置の必須条件である絶対値測定(同一溶液なら、ほぼ同じ値で計測される)とする精度の高い測定装置としての所期の目的を達成したのである。
(2) According to the above (1), using the means for measuring redox potential (ORP) of human saliva with an indicator electrode (platinum) loaded on the bottom of the sample tank, it has already been certified as a medical device by the Ministry of Health, Labor and Welfare. With the cooperation of Dr. Mieko Okazawa, a pediatric physician in Yokohama who proved the physical condition by mV value limitation in saliva clinical practice that exceeds 8,000 cases using the human saliva ORP measuring device. Measurement of saliva ORP, which is the same solution of 20 people including Table 1 shows each storage-type sample tank provided in the apparatus of the present invention in order to establish a calibration standard solution used as a calibration standard solution in order to establish a calibration standard solution used for checking the performance of the ORP electrode. A comparison of ORP measurement accuracy was attempted using a 1 mol KCl solution in the BOX 1, the sample tank BOX 2, and the sample tank BOX 3. As a result, as shown in Table 1, the measurement results of 5 times each for 5 days with different dates and times show a reproducible value within ± 5 mV with reference to plus 60 mV, and it is not considered that it coincided by chance. It achieved the intended purpose as a calibration standard solution for the ORP quantification determination device and method of use of the invention, and was an essential condition of the saliva ORP measurement device (measured with almost the same value for the same solution) The intended purpose as a highly accurate measuring device is achieved.
（３）請求項１至る請求項１０も前記（１）〜（２）と同様に、基準電極、銀―塩化銀電極KCｌ １molを格納する１５ 内部液を格納する液槽の上部蓋となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ１molの溶液を介して連通させるようにしている。
上部蓋となる下面部に細溝を設けたことにより前記の通り基準電極、銀―塩化銀電極KCｌ １molが接触する液絡部と試料槽部の底に内蔵された指示電極の三者間が常に一定の距離を設けられ、測定対象試料とする溶液含浸のみの酸化還元電位ORP値（ｍV）が安定して電子授受交換ができるようにした。本発明の測定装置は、表2に示す通り、収納式の試料槽ＢＯＸ1、試料槽ＢＯＸ２、至る試料槽ＢＯＸ３で、同一検体者の小唾液腺および局所唾液成分が混合された、口腔内の舌の上に綿棒を乗せ口を閉じて採取した総合唾液成分のＯＲＰ値（ｍＶ）の測定値差がプラスマイナス５ｍＶ以内で再現され、偶然に一致したとは考えられず、各収納式の試料槽ＢＯＸを用いた唾液ＯＲＰ測定において、本発明のORP数値化判定装置および使用方法によって、麻薬などの薬物を体内に取り込んだことでの微妙に体内を酸化傾向に導く反応を正確に捉えることを立証するために、唾液の酸化還元電位（ORP）による延べ８,０００症例の数値限定で体内の酸化と還元の度数分布を確立した小児内科医の岡澤美江子医師の測定協力のもと、その実施結果を表１至る表６で後述する。測定対象試料とする麻薬などの薬物を摂取したことによる、唾液ＯＲＰ値（ｍＶ）が酸化有意の強い酸化のストレス状態であるか、又は体内が還元有意の酸化のストレスが無い状態であるか否かのスクリーニング活用を可能にしたのである。
(3) Similarly to the above (1) to (2), in claim 10 leading to claim 1, 15 stores the reference electrode and the silver-silver chloride electrode KCl 1 mol 15 The lower surface serving as the upper lid of the liquid tank storing the internal liquid A narrow groove is provided in the part so that the electronic conduction between both the reference electrode and the indicator electrode is communicated via a KCl 1 mol solution.
By providing a narrow groove in the lower surface part which becomes the upper lid, the reference electrode, the liquid junction where 1 mol of silver-silver chloride electrode KCl 1 mol contacts, and the indicator electrode built in the bottom of the sample tank as described above A constant distance is always provided, and the redox potential ORP value (mV) of only the solution impregnation as a measurement target sample can be stably exchanged. As shown in Table 2, the measuring device of the present invention is a storage-type sample tank BOX1, sample tank BOX2, and sample tank BOX3. The small salivary glands and local saliva components of the same subject are mixed, and the tongue in the oral cavity is mixed. The difference in the ORP value (mV) of the total saliva component collected by placing a cotton swab on top and closing the mouth was reproduced within plus or minus 5 mV, and it was not considered coincidental. In the salivary ORP measurement using an urine, it is proved that the ORP digitization determination device and the method of use of the present invention accurately capture the reaction that subtly causes the body to oxidize due to the incorporation of drugs such as narcotics. Therefore, with the cooperation of measurement by Dr. Mieko Okazawa, a pediatric physician who established the frequency distribution of oxidation and reduction in the body by limiting the numerical value of 8,000 cases by the redox potential (ORP) of saliva. Table 1 to Later in Table 6. Whether or not the saliva ORP value (mV) is due to ingestion of a drug such as narcotics as a measurement target sample, or is in a state where there is no significant oxidation stress in the body This made it possible to use such screening.
又、基準電極、銀―塩化銀電極KCｌ １mol内部液を格納する液槽の上部蓋となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ１molの溶液を介して連通させたことで、より精緻な酸化還元電位測定装置となり、微妙な酸化還元反応を正確にとらえることができ、一例として測定対象試料の応用は、測定対象試料とする綿棒に精製水（純水）を含浸させたＯＲＰ値（ｍＶ）の測定と、同じく綿棒に精製水（純水）を含浸させ、測定場所の空気又は測定対象とする場所の土壌を所定時間触れさせた状態にするなど、多岐にわたって測定対象試料の酸化還元電位の測定が可能となったのである。又、表3至る表６で示す通り、耳下腺左右唾液と舌の上で採取の総体唾液のストレス値差が明確になるORP数値化判定装置および使用方法である。 The reference electrode and silver-silver chloride electrode KCl 1 mol The inner surface of the liquid tank for storing the liquid is provided with a narrow groove on the lower surface, and the reference electrode and the indicator electrode are electrically connected via the KCl 1 mol solution. This makes it possible to obtain a more precise oxidation-reduction potential measurement device and accurately capture subtle oxidation-reduction reactions. As an example, the application of the sample to be measured includes purified water (pure water) Measurement of ORP value (mV) impregnated with water) and impregnation of purified water (pure water) into a cotton swab, and letting the air at the measurement location or the soil at the measurement location touch for a predetermined time, etc. Thus, it has become possible to measure the oxidation-reduction potential of a sample to be measured over a wide range. In addition, as shown in Table 6 leading to Table 3, the ORP quantification determination apparatus and method of use in which the stress value difference between the parotid left and right saliva and the total saliva collected on the tongue is clarified.
本発明のORP数値化判定装置および使用方法を用いてのヒト唾液である同一検体液の
各収納式の試料槽ＢＯＸ１至る試料槽ＢＯＸ３の測定結果。ORP単位（ｍV）。
The measurement result of sample tank BOX3 to each storage-type sample tank BOX1 of the same sample liquid which is human saliva using the ORP digitization determination apparatus and usage method of the present invention. ORP unit (mV).
農薬不使用の植物エキスから抽出の醗酵成分ブレンド壁材を使用して施工した室内での
体感前後のヒト唾液ＯＲＰ経時変化。ORP単位（ｍV）。
Changes in human saliva ORP over time before and after bodily sensation in a room constructed using a fermentation component blend wall material extracted from a plant extract that does not use agricultural chemicals. ORP unit (mV).
シンナー系溶剤を混ぜての壁材を使用して施工した室室内での
体感前後のヒト唾液ＯＲＰ経時変化。ORP単位（ｍV）。
Changes in human saliva ORP over time before and after experiencing in a room constructed using a wall material mixed with a thinner solvent. ORP unit (mV).
無農薬栽培レモンで、保存に防腐剤、カビ防止剤、乾燥防止剤など不使用の
レモン摂取前後の唾液の経時変化。ORP単位（ｍV）。
Changes in saliva over time before and after ingesting lemons that are non-pesticide-grown lemons and are not used for preservatives, fungicides, anti-drying agents, etc. ORP unit (mV).
農薬栽培のレモンで、保存に防腐剤、カビ防止剤、乾燥防止剤などを使用した
レモン摂取前後の唾液の経時変化。ORP単位（ｍV）。
Pesticide-grown lemons used preservatives, fungicides, anti-drying agents, etc. for storage
Changes in saliva over time before and after ingestion of lemon. ORP unit (mV).
表３、表４及び表５、表６について説明する。表３は農薬不使用の植物エキスから抽出の醗酵成分ブレンド壁材部屋での体感前後の唾液の経時変化である。表４はシンナー系使用の室内壁材塗料の部屋での体感前後の唾液の経時変化である。表５は無農薬栽培レモンで、保存に防腐剤、カビ防止剤、乾燥防止剤など不使用のレモン摂取前後の唾液の経時変化である。表６は農薬栽培のレモンで、保存に防腐剤、カビ防止剤、乾燥防止剤などを使用した レモン摂取前後の唾液の経時変化である。この表３至る表６で示されたとおり、住まいの壁材に使用した実例及び果実に使用した実例にあるように、化学物質の体内への影響が、ほぼ同様に酸化反応を示したことで、本発明の本発明のORP数値化判定装置および使用方法が、測定対象試料とする麻薬などの薬物を摂取したことによる、体内が酸化有意の強い酸化状態であるか、又は還元有意の極めて弱い酸化状態か否かのスクリーニング活用が期待できる精度の高い測定装置としての所期の目的を達成したのである。 Table 3, Table 4, Table 5, and Table 6 will be described. Table 3 shows changes over time in saliva before and after experiencing in a fermentation component blend wall material room extracted from a plant extract that does not use agricultural chemicals. Table 4 shows changes with time of saliva before and after the experience in the room of the interior wall material paint using the thinner system. Table 5 shows the time-dependent changes in saliva before and after ingesting lemons that were not used for preservatives, such as preservatives, fungicides, and anti-drying agents. Table 6 shows the changes in saliva over time before and after ingesting lemons, which were preserved with pesticide-grown lemons and used preservatives, fungicides, anti-drying agents, etc. for storage. As shown in Table 6 leading to Table 3, as shown in the examples used for the wall material of the house and the examples used for the fruit, the effects of chemical substances on the body showed an oxidation reaction almost similarly. The ORP quantification determination apparatus and method of use of the present invention are those in which the body is in a highly oxidized state with significant oxidation due to the ingestion of drugs such as narcotics to be measured, or the reduction is extremely weak. This achieves the intended purpose of a highly accurate measuring device that can be expected to be used for screening whether it is in an oxidized state or not.
本発明者は麻薬と呼ばれる物の中には覚醒剤、大麻、アヘン、などの常用者の唾液臨床ができない状況から、唾液の酸化還元電位（ORP）による延べ８,０００症例の数値限定で体内の酸化と還元の度数分布を確立した小児内科医の岡澤美江子医師の測定協力のもと、表３至る表６に見られるように化学物質によって、人間の生体反応は、明確に唾液成分が強い酸化反応を示したのである。すなわち、化学物質により体内は大量に活性酸素が発生していることは明白であり、よって、表３至る表６で示したとおり人間の唾液ORPの酸化反応の経時変化が偶然に一致したとは考えられず、シンナー系の化学物質を使用した室内空気の体内摂取と農薬などの化学物質が含まれた果実の体内摂取において、本発明である基準電極、銀―塩化銀電極KCｌ １molの内部液を格納する液槽の上部蓋となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ１molの溶液を介して連通させるようにして、電子移動をスムースにできると考察した結果は、前述表1至る表6で示した通りである。 Since the present inventor cannot perform saliva clinical trials for regular users such as stimulants, cannabis, opium, etc. among the so-called narcotics, the number of cases in the body is limited to a total of 8,000 cases due to the redox potential (ORP) of saliva. Under the cooperation of measurement by Dr. Mieda Okazawa, a pediatric physician who has established the frequency distribution of oxidation and reduction, human biological reactions are clearly strong in salivary components due to chemical substances as shown in Table 3 to Table 6. It showed an oxidation reaction. In other words, it is clear that active oxygen is generated in large quantities in the body due to chemical substances. Therefore, as shown in Table 6 to Table 3, the time-dependent changes in human salivary ORP oxidation coincided by chance. Unexpectedly, in the intake of indoor air using thinner chemicals and the intake of fruits containing chemicals such as agricultural chemicals, the internal solution of the reference electrode, 1 mol of silver-silver chloride electrode KCl, which is the present invention When a thin groove is provided in the lower surface part that serves as the upper lid of the liquid tank for storing the reference electrode and the indicator electrode, the electronic conduction between the two electrodes is communicated via a KCl 1 mol solution, thereby smoothing the electron transfer. The result of consideration is as shown in Table 6 leading to Table 1 above.
被験者の唾液を含浸させた綿棒を差し込む試料槽部を設けたORP数値化判定装置および使用方法である。請求項に述べている、麻薬と呼ばれる物の中には覚醒剤、大麻、アヘン、モルヒネ、ヘロイン、MDMA・MDA、コカイン、シンナー等有機溶剤、危険ドラッグなどがあり、これらの摂取乱用により、体内細胞への酸化反応が強くなる。大麻などの植物による神経細胞の麻痺は幻覚症状であり、正常な生体機能をクラッシュさせ酸化となる。さらに、覚せい剤は人工の化学物質を混ぜたメタンフェタミンであり、これらの人工的な化学物質を体内に取り込むことで、体内は大量の強い活性酸素が生成されることが考察されるとする理由を表３至る表６で証明したのであり、血液を通して唾液の酸化還元反応が、飲食又は体感後において最速で３０分経過後に色濃く現れる生体反応の特性を捉え、覚せい剤反応のスクリーニング活用が大いに期待できることに着眼したのである。 It is an ORP digitization determination apparatus provided with a sample tank portion into which a cotton swab impregnated with saliva of a subject is inserted and a method of use. Some of the so-called narcotics mentioned in the claims include stimulants, cannabis, opium, morphine, heroin, MDMA / MDA, cocaine, thinner, and other organic solvents, dangerous drugs, and so on. The oxidation reaction to becomes stronger. The paralysis of nerve cells by plants such as cannabis is a hallucinatory symptom, which crashes normal biological functions and becomes oxidized. Furthermore, the stimulant is methamphetamine mixed with artificial chemicals, and it is considered that a large amount of strong active oxygen is generated in the body by taking these artificial chemicals into the body. As proved in Table 6 through Table 3, the saliva redox reaction through the blood captures the characteristics of the biological reaction that appears dark after 30 minutes at the fastest after eating, drinking, or bodily sensation, and the screening utilization of the stimulant reaction can be greatly expected I focused on.
図１で示す通り、基準電極、銀―塩化銀電極KCｌ １molが接触する液絡部と試料槽部の底に内蔵された指示電極の三者間が常に一定の距離を設けられ、測定中に指示電極が溶液含浸綿棒に覆われることで空気に触れて酸化還元の緩衝を受けずに、測定対象試料とする溶液含浸のみの酸化還元電位ORP値（ｍV）が安定して電子授受交換ができるようにしているのである。本発明に測定装置に用いる内部液の濃度は、KCｌ １molに限定されない、 As shown in Fig. 1, there is always a fixed distance between the reference electrode, the liquid junction where 1 mol of silver-silver chloride electrode KCl contacts, and the indicator electrode built in the bottom of the sample tank. Since the indicator electrode is covered with a solution-impregnated cotton swab, the oxidation-reduction potential ORP value (mV) of only the solution impregnation used as a measurement target sample can be stably exchanged without touching the air and receiving buffer for oxidation-reduction. It is doing so. The concentration of the internal liquid used in the measuring apparatus according to the present invention is not limited to 1 mol KCl.
つぎに、本発明の図２で示す通り、基準電極、銀―塩化銀電極KCｌ １molを格納する１５ 内部液を格納する液槽の上部蓋となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ １molの溶液を介して連通させるようにした構成図である。これは浴槽室内において天井の溝に水滴が足る速い動きからの着眼で、内部液を格納する液槽の上部蓋となる下面部に細溝を設けたことは浴槽室内の天井に溝をほどこしたことと同じになり、液絡部からのKCｌ １molの流出で起きる結晶化が極少にでき、唾液含浸の綿棒を測定の試料槽部に差し込むことで毛細管現象を利用してのKCｌ １mol 溶液の流出が最小で抑えられ、測定対象試料であるプラスマイナスの電子移動速度が速くなり、安定した再現精度測定は、表１及び表２で示されたとおりである。 Next, as shown in FIG. 2 of the present invention, a reference electrode, a silver-silver chloride electrode KCl 1 containing 15 mol, a narrow groove is provided in the lower surface portion serving as an upper lid of the liquid tank containing the internal liquid, and the reference electrode and instructions It is the block diagram which made the electronic conduction of the both electrodes which are electrodes communicate through the solution of 1 mol of KCl. This is because of the quick movement of water droplets in the ceiling groove in the bathtub room, and the provision of a narrow groove on the bottom surface, which is the upper lid of the liquid tank that stores the internal liquid, gave the groove to the ceiling in the bathtub room. In the same way, the crystallization caused by the outflow of 1 mol of KCl from the liquid junction can be minimized, and the outflow of the 1 mol solution of KCl using capillary action by inserting a saliva-impregnated cotton swab into the sample tank for measurement. Is suppressed to a minimum, the plus / minus electron transfer speed of the sample to be measured is increased, and stable reproducibility measurement is as shown in Tables 1 and 2.
本発明を実施するための最良の形態について説明する。内部液を格納する液槽の上部蓋
となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ １molの溶液を介して連通させるようにしたORP数値化判定装置および使用方法である。唾液の生体反応は酸化体と還元体の電子の授受であり、電子の流れは負極（マイナス）から正極（プラス）となり、マイナス電子を奪われ酸化することを意味する。負極（マイナス）電子が放出（奪われた）場合は酸化反応であり、反対に起こっている正極（プラス）が負極（マイナス）電子を受け取る場合は還元反応である。人間の体は常に負極（マイナス）電子が奪われる自然摂理で構成され、酸化反応をいかに抑えるかが健康で生きるための大切な要件である。
The best mode for carrying out the present invention will be described. An ORP quantification judgment device that has a narrow groove in the lower surface part that serves as the upper lid of the liquid tank that stores the internal liquid, and allows the electronic conduction of both the reference electrode and the indicator electrode to communicate with each other via a 1 mol solution of KCl. And how to use. The biological reaction of saliva is the exchange of electrons between an oxidant and a reductant, and the flow of electrons changes from the negative electrode (minus) to the positive electrode (plus), meaning that negative electrons are taken away and oxidized. When the negative electrode (minus) electron is released (stolen), it is an oxidation reaction, and when the positive electrode (plus) which is taking place on the contrary receives the negative electrode (minus) electron, it is a reduction reaction. The human body is always made up of natural providence where negative (negative) electrons are taken away, and how to suppress the oxidation reaction is an important requirement for a healthy life.
図１について説明する。８は本発明のORP数値化判定装置および使用方法において、測
定装置の試料槽の底部に装填の指示電極と基準電極の内部液格納の溶液を介しての電子
授受の構成の断面図であり、９は酸化還元電位差計を有し、１０は指示電極と参照電極導通のリード線であり、１１は参照電極となる銀電極とし、１２は試料槽部土台、１３は試料槽部上面、１４試料槽部上部浅瀬部分であり、１５は内部液を格納する液槽の上部ふたを設け、１６は内部液を格納する液槽の上面であり、１７には塩化カリウムが格納されてる。１８は基準電極の銀線ＫＣl １mol内部液槽の蓋となる、下面部に細溝が連通し、１９は銀線が内蔵され、
２０は銀線を包み込む銀粉であり、２１は内部液を格納する液槽のＫＣＬ溶液が漏れないようにパッキンを挟み込むようにしている。２２はガラスやカーボン等の不活性素材からなる筒部を設け、２３はコットンを詰め込み銀粉が流出しないようにしている。２４は綿棒などの多孔性物質を差込む縦筒穴の底部であり接着ボンドで４の白金電極を固定している。２５は指示電極棒状の下部をナットで締めるようにしてゆるみが出ないようにしている。２６は試料槽部に差し込む綿棒などの多孔性物質で構成される持ち手部が木軸及び紙軸であり、２７は綿棒の綿部分に含浸の被検液とKCｌ １mol溶液が接触する液絡部であり、２８は指示電極と綿棒に含浸の唾液とKCｌ １mol溶液が接する液絡部への導通イメージであり、２９は指示電極の頭部を丸み形状をもたせ唾液含浸綿棒との接触部で構成している。
With reference to FIG. 8 is a cross-sectional view of the configuration of electron exchange through the solution stored in the internal liquid of the indicator electrode and the reference electrode loaded in the bottom of the sample tank of the measuring device in the ORP digitization determination apparatus and method of use of the present invention; 9 is an oxidation-reduction potentiometer, 10 is a lead wire for conducting the indicator electrode and the reference electrode, 11 is a silver electrode serving as a reference electrode, 12 is a base for the sample tank, 13 is the top of the sample tank, and 14 samples An upper shallow portion of the tank portion, 15 is provided with an upper lid of the liquid tank for storing the internal liquid, 16 is an upper surface of the liquid tank for storing the internal liquid, and 17 stores potassium chloride. 18 is a lid of a 1 mol silver wire KCl 1 mol internal liquid tank of the reference electrode, and a narrow groove communicates with the lower surface portion, 19 is a silver wire built-in,
Reference numeral 20 denotes silver powder that wraps the silver wire, and reference numeral 21 denotes a packing that prevents the KCL solution in the liquid tank that stores the internal liquid from leaking. 22 is provided with a cylindrical portion made of an inert material such as glass or carbon, and 23 is filled with cotton so that silver powder does not flow out. Reference numeral 24 denotes a bottom portion of a vertical cylindrical hole into which a porous material such as a cotton swab is inserted, and 4 platinum electrodes are fixed by an adhesive bond. No. 25 prevents the looseness from occurring by tightening the lower part of the indicator electrode bar with a nut. Reference numeral 26 is a handle made of a porous material such as a cotton swab inserted into the sample tank, and a wooden shaft and a paper shaft are provided. 27 is a liquid junction where the test solution impregnated with the cotton portion of the cotton swab is in contact with the 1 mol KCl solution 28 is an image of conduction to the liquid junction where the saliva impregnated on the indicator electrode and the cotton swab is in contact with the 1 mol solution of KCl, and 29 is the contact portion with the saliva-impregnated cotton swab by rounding the head of the indicator electrode. It is composed.
図２について説明する。図２において８の本発明の１０は指示電極と参照電極導通のリード線であり、１１は参照電極となる銀電極とし、１５は内部液を格納する液槽の上部ふたを設け、１６は内部液を格納する液槽の上面である。１８は基準電極の銀線ＫＣＬ溶液導通の細溝とし、内部液を格納する液槽の上部ふたの下面部に細溝を設けることで測定対象試料の電子導通が加速され安定した酸化還元電位測定が得られることを前記表１及び表2で示した通りである。 With reference to FIG. In FIG. 2, 10 of the present invention 8 is a lead wire for conducting the indicator electrode and the reference electrode, 11 is a silver electrode as a reference electrode, 15 is provided with an upper lid of a liquid tank for storing the internal liquid, and 16 is an internal electrode. It is the upper surface of the liquid tank which stores a liquid. Reference numeral 18 is a narrow groove for conducting the silver wire KCL solution of the reference electrode, and by providing a narrow groove on the lower surface of the upper lid of the liquid tank for storing the internal liquid, the electronic conduction of the sample to be measured is accelerated and stable redox potential measurement. As shown in Table 1 and Table 2 above.
本発明の測定装置は、1台の装置で、舌の上に集まる総合唾液および還元有意に導くアミラーゼ、パロチン、ラクトペルオキシターゼ、ヒスタチンなどを主流とする酸化影響を受けやすい耳下腺左右から分泌される唾液成分3か所のヒト唾液成分の酸化還元電位値（ｍＶ）比較測定を可能にし、２０２０年のオリンピックにおいてのスクリーニング検査。各国選手は国民性の違いから下着を脱いでのドーピング検査に抵抗があり、検査時間がかかることから、唾液による簡便な行為でスクリーニング検査活用で、疑わしき場合には、尿、血液検査など絞り込んだ検査体制に進ませることが可能となり、早期の覚せい剤乱用阻止と一般市民の社会生活、集団組織の規律を遵守することなど産業上での利用は大いに期待できるのである。
The measuring device of the present invention is a single device that is secreted from the left and right of the parotid gland, which is susceptible to oxidative effects such as total saliva collected on the tongue and amylase, parotin, lactoperoxidase, histatin, etc. This enables screening of redox potential (mV) of human saliva components in three locations, and screening tests at the 2020 Olympics. Players from each country are resistant to doping tests after taking off their underwear due to differences in national character, and it takes a long time to test. By using a screening test with a simple act of saliva, in case of doubt, we narrowed down urine and blood tests. It is possible to proceed to the inspection system, and industrial use such as prevention of stimulant drug abuse at an early stage, social life of the general public, and compliance with group organization regulations can be greatly expected.
８ 試料槽の指示電極と基準電極の内部液格納の溶液を介しての電子授受の構成の断面図
９ 酸化還元電位差計
１０ 指示電極と参照電極導通のリード線
１１ 参照電極となる銀電極
１２ 試料槽部土台
１３ 試料槽部上面
１４ 試料槽部上部浅瀬部分
１５ 内部液を格納する液槽の上部ふた面
１６ 内部液を格納する液槽の上面
１７ 塩化カリウム
１８ 基準電極の銀線ＫＣl １mol内部液槽の蓋となる、下面部に細溝が連通
１９ 銀線
２０ 銀線を包み込む銀粉
２１ 内部液を格納する液槽のＫＣl １mol溶液が漏れないようにパッキンを挟み込む
２２ ガラスやカーボン等の不活性素材からなる筒部
２３ コットン
２４ 綿棒などの多孔性物質を差込む縦筒穴の底部は接着ボンドで白金電極を固定
２５ 指示電極棒状の下部をナットで試料槽の底部に固定の白金電極を締める
２６ 試料槽部に差し込む綿棒などの多孔性物質で構成される持ち手部が木軸及び紙軸
２７ 綿棒の綿部分に含浸の被検液とＫＣl １mol溶液が接触する液絡部
２８ 指示電極と綿棒に含浸された唾液溶液とＫＣl １mol溶液が接触する液絡部への導通部
２９ 指示電極先端部を丸み形状として唾液含浸綿棒との接触部
8 Cross-sectional view of the configuration of electron exchange through the solution stored in the internal solution of the indicator electrode and the reference electrode of the sample tank 9 Redox potentiometer 10 Lead wire for indicating electrode and reference electrode conduction 11 Silver electrode 12 serving as reference electrode Sample Tank base 13 Sample tank upper surface 14 Sample tank upper shallow part 15 Upper lid surface of the liquid tank for storing the internal liquid 16 Upper surface of the liquid tank for storing the internal liquid 17 Potassium chloride 18 Silver wire KCl 1 mol of the reference electrode internal liquid A narrow groove communicates with the bottom of the tank, which is a lid for the tank. 19 Silver wire 20 Silver powder that wraps the silver wire 21 Packs the packing so that the 1 mol solution of KCl in the liquid tank that contains the internal liquid does not leak 22 Inactive glass or carbon Tube made of material
23 Cotton 24 The bottom of the vertical cylindrical hole into which a porous material such as a cotton swab is inserted is fixed with an adhesive bond to fix the platinum electrode. 25 The lower part of the indicator electrode bar is fastened to the bottom of the sample tank with a nut. The handle composed of a porous material such as a cotton swab is inserted into a wooden shaft and a paper shaft. 27 The liquid junction portion where the test liquid impregnated with the cotton portion of the cotton swab contacts the 1 mol solution of KCl.
28 Conductive part to liquid junction where saliva solution impregnated with indicator electrode and cotton swab contacts with KCl 1 mol solution 29 Contact part with saliva-impregnated cotton swab with rounded tip of indicator electrode
本発明はＯＲＰ数値化判定装置および使用方法にかんする。
The present invention relates to an ORP digitization determination apparatus and method of use.
酸化還元電位測定装置は指示電極と参照電極を備え、指示電極と参照電極を試料溶液中に挿入し、溶液中の電解質の濃度や酸化体・還元体のイオン濃度比など酸化還元電位を測定する。従来、酸化還元電位の測定は主に排水やプラント水の水質検査など多量の溶液を対象とする一方、試料溶液が少量で、緩衝能力が低い場合、測定の再現精度が不安定で正確性にかけるという欠点があった。液絡部を通して試料溶液中に流出する参照電極（銀−塩化銀）側の内部液が試料溶液と接触することによって酸化還元電位が測定されるのであるが、指示電極とする白金電極との距離が近いことにより検体液以外の参照電極（銀−塩化銀）側の内部液が試料槽部に装填される指示電極に接触してしまうことで緩衝能力の低い試料溶液（唾液などの生体液等）のような場合、参照電極の内部液の流出がわずかであっても検体溶液に対する影響が大きく、指示電極に直接接触した場合は、検体溶液の測定ではなく、内部溶液に影響された測定となり正確な酸化還元電位測定ができないからである。 The oxidation-reduction potential measuring device includes an indicator electrode and a reference electrode, and the indicator electrode and the reference electrode are inserted into the sample solution, and the oxidation-reduction potential such as the concentration of the electrolyte in the solution and the ion concentration ratio of the oxidant / reductant is measured. . Conventionally, redox potential measurement is mainly for large quantities of solutions such as water quality inspection of wastewater and plant water. On the other hand, if the sample solution is small and the buffer capacity is low, the reproducibility of measurement is unstable and accurate. There was a drawback of calling. The redox potential is measured when the internal solution on the side of the reference electrode (silver-silver chloride) flowing out into the sample solution through the liquid junction comes into contact with the sample solution, but the distance from the platinum electrode as the indicator electrode Sample solution with low buffering capacity (biological fluid such as saliva etc.) due to the internal solution on the reference electrode (silver-silver chloride) side other than the sample solution coming into contact with the indicator electrode loaded in the sample tank ), Even if the internal solution of the reference electrode is small, it has a large effect on the sample solution. If it is in direct contact with the indicator electrode, the measurement is influenced by the internal solution, not the sample solution. This is because an accurate redox potential cannot be measured.
特許文献１は、測定電極及び比較電極を被検液に浸漬して被検液の酸化還元電位を測定する酸化還元電位測定装置の電極を検査する方法、及びその検査に使用する標準液に関するものを提供しているものである。
Patent Document 1 relates to a method for inspecting an electrode of an oxidation-reduction potential measuring device for measuring the oxidation-reduction potential of a test solution by immersing a measurement electrode and a comparison electrode in the test solution, and a standard solution used for the inspection It is something that provides.
特許文献２は、過硫酸塩を活性炭により還元処理する過硫酸塩処理装置、過硫酸塩処理方法の技術に関するものである。
Patent Document 2 relates to a technique for a persulfate treatment apparatus and a persulfate treatment method for reducing persulfate with activated carbon.
特許文献３及び特許文献４とも本出願人の発明による人間の生体液である唾液の酸化還元電位を測定する装置について提供している。 Patent Documents 3 and 4 both provide an apparatus for measuring the redox potential of saliva, which is a human biological fluid, according to the applicant's invention.
本発明は、特許文献３及び特許文献４で示す通り、酸化還元電位測定装置は指示電極と参照電極を備え、指示電極と参照電極を試料溶液中に挿入し、溶液中の電解質の濃度や酸化体・還元体のイオン濃度比など酸化還元電位を測定する人間の唾液の酸化還元電位測定装置であるが、検体液を含浸させた綿棒を接触させる試料槽部に新しい検体液の測定ごとに綿棒に添えて丸棒形状の指示電極を装填することで酸化還元電位を測定するのであるが、測定装置本体の外付け装着の白金電極においては、測定装置使用者の取り扱いが粗雑な場合は、丸棒形状の白金電極を折り曲げ微細な傷をつける問題が生じることになり、それにより、測定精度の大きな値差が生じるのである。
In the present invention, as shown in Patent Document 3 and Patent Document 4, the oxidation-reduction potential measuring device includes an indicator electrode and a reference electrode, the indicator electrode and the reference electrode are inserted into a sample solution, and the concentration and oxidation of the electrolyte in the solution are measured. This is a device for measuring the redox potential of human saliva that measures the redox potential such as the ion concentration ratio of the body / reductant. In addition, the redox potential is measured by loading a round bar-shaped indicator electrode with a platinum electrode attached externally to the measuring instrument body. There arises a problem that the rod-shaped platinum electrode is bent and finely scratched, thereby causing a large value difference in measurement accuracy.
従来においては、ORP電極の性能チェックに用いる校正標準溶液においては、酸化体と還元体が共存しない、溶存酸素（DO）、すなわち水質に含む酸素の量が極めて少ないキンシドロン液など、空気に触れても酸化側にも還元側にも傾かない比較的安定しているとしてキンシドロン溶液を用いてORP電極の性能チェックを行うための標準溶液が使用されている。
Conventionally, calibration standard solutions used to check the performance of ORP electrodes are exposed to air, such as quincidrone solution in which the oxidant and reductant do not coexist, dissolved oxygen (DO), that is, the amount of oxygen contained in the water is extremely small. A standard solution is used to check the performance of the ORP electrode using a quincidron solution because it is relatively stable, neither inclined nor oxidized or reduced.
当発明のORP数値化判定装置および使用方法においては、測定対象試料に酸化体と還元体が混在している試料液であり、この酸化体と還元体の電子授受の電位差を計測するのであり、酸化体と還元体が共存しない、すなわち、溶存酸素（DO）が存在しない溶液を用いてORP電極の性能チェックでは、本発明のORP数値化判定装置および使用方法の校正標準溶液の要件を果たせないと考える。
In the ORP digitization determination apparatus and method of use of the present invention, it is a sample liquid in which an oxidant and a reductant are mixed in a sample to be measured, and the potential difference in electron transfer between the oxidant and the reductant is measured. In the ORP electrode performance check using a solution in which the oxidant and the reductant do not coexist, that is, there is no dissolved oxygen (DO), the requirement of the calibration standard solution of the ORP digitization judgment device and the method of use of the present invention cannot be fulfilled. I think.
この課題を解決するため本発明にかかる酸化還元電位測定装置は、検体液である人間の唾液採取の綿棒、又は各種水溶液を含浸させた綿棒を参照電極と指示電極に介在させることで、溶液中の電解質の濃度や酸化体・還元体のイオン濃度比など酸化還元電位を測定する構成としている。 In order to solve this problem, the oxidation-reduction potential measuring device according to the present invention includes a swab for collecting human saliva, which is a sample solution, or a cotton swab impregnated with various aqueous solutions, interposed between a reference electrode and an indicator electrode. It is configured to measure the redox potential such as the concentration of the electrolyte and the ion concentration ratio of the oxidant / reductant.
図１で示す通り、基準電極、銀―塩化銀電極KCｌ １mol（KCl 内部液濃度は限定されない）が接触する液絡部と試料槽部の底に内蔵された指示電極の三者間が常に一定の距離を設けられ、測定中に指示電極が溶液含浸綿棒に覆われることで空気に触れて起こる酸化還元の緩衝を受けずに、測定対象試料とする溶液含浸のみの酸化還元電位ORP値（ｍV）が安定して電子授受交換ができるように構成されている。 As shown in Fig. 1, the reference electrode and the silver-silver chloride electrode KCl 1 mol (the concentration of the KCl internal solution is not limited) are always in contact with the three parts of the indicator electrode built in the bottom of the sample tank. A constant distance is provided, and the indicator electrode is covered with a solution-impregnated cotton swab during measurement. mV) can be exchanged electronically stably.
つぎに、図2で示す通り、基準電極、銀―塩化銀電極KCｌ １mol（KCl 内部液濃度は限定されない）を格納する１５ 内部液を格納する液槽の上部蓋となるうえ下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ１molの溶液を介して連通させるようにしたことにより、指示電極は空気に触れて酸化影響を受けることなくKCl溶液を通してプラスマイナスの電子導通が加速されることの証としての表1、表2のデータによって後述する。 Next, as shown in Fig. 2, the reference electrode, silver-silver chloride electrode KCl 1mol (KCl internal liquid concentration is not limited) is stored. By connecting the reference electrode and the indicator electrode through the KCl 1 mol solution, the indicator electrode is exposed to air and is not affected by oxidation. This will be described later with the data in Tables 1 and 2 as proof that conduction is accelerated.
なぜなら、浴槽の天井の水滴の動きは天井平面より、天井側に溝を掘ってある箇所ほど早い速度で流れることに注目し、肉眼では目視できない電子の流れも引力に引き付けられる状態でありながら、より移動速度は高まり電子授受が安定し、酸化還元電位測定の再現精度を高められと考察したのである。 Because the movement of water drops on the ceiling of the bathtub flows faster at the location where the groove is dug on the ceiling side than the ceiling plane, while the flow of electrons that can not be seen with the naked eye is also attracted to the attractive force, He thought that the transfer speed would be higher, the electron transfer would be stable, and the reproducibility of the redox potential measurement could be improved.
上記目的を達成するために、本発明は（請求項１の構成要件）とで、本発明の溶液中の電解質の濃度や酸化体・還元体のイオン濃度比など酸化還元電位を測定する構成であり、前記浴槽の天井の水滴の動きは天井平面より、天井側に溝を掘ってある箇所ほど早い速度で流れることに注目し、肉眼では目視できない電子の流れも引力に引き付けられる状態でありながら、より移動速度は高まり電子授受が安定し、酸化還元電位測定の再現精度を高められることを突き止め、表１に示す通り、本発明のORP数値化判定装置および使用方法の校正標準溶液の要件を果たせるようにしたのである。
In order to achieve the above object, the present invention is configured to measure an oxidation-reduction potential such as the concentration of the electrolyte in the solution of the present invention and the ion concentration ratio of the oxidant / reductant. Yes, note that the movement of water drops on the ceiling of the bathtub flows faster at the location where the groove is dug on the ceiling side than the ceiling plane, and the flow of electrons that cannot be seen with the naked eye is also attracted to the attractive force As a result, it was found that the transfer speed was increased, the electron transfer was stable, and the reproducibility of the redox potential measurement could be improved. I was able to do it.
以上の説明から明らかなように、本発明にあっては次に列挙する効果が得られる。
（１）（請求項１の構成要件）とで構成されているので、人間の唾液の酸化還元電位（ＯＲＰ）測定手段を用いて、本発明の有効性を証明するために、本発明者が取得している特許をもとに開発した厚生労働省から医療機器として認証されている人間の唾液ＯＲＰ測定装置と本発明のORP数値化判定装置および使用方法により、本発明で用いる校正標準溶液とする溶液は、KCｌ １mol溶液により測定精度の比較を試みたのである。
As is clear from the above description, the present invention has the following effects.
In order to prove the effectiveness of the present invention using the means for measuring redox potential (ORP) of human saliva, the present inventor The calibration standard solution used in the present invention is based on the human saliva ORP measurement device and the ORP quantification judgment device and method of use of the present invention that have been certified as medical devices by the Ministry of Health, Labor and Welfare developed based on the patents that have been acquired. The solution was an attempt to compare the measurement accuracy with a 1 mol KCl solution.
（２）前記（１）によって、試料槽部の底に指示電極（白金）を装填した人間の唾液の酸化還元電位（ＯＲＰ）測定手段を用いて、すでに、厚生労働省から医療機器として認証されている人間の唾液ＯＲＰ測定装置を用いて８，０００症例を超える唾液臨床でｍＶ数値限定による体調度を立証した横浜・小児内科医の岡澤美江子医師の協力のもと、患者及び付き添いの健
常者を含む２０人の同一溶液である唾液ＯＲＰの測定を実施した。表１は、ORP電極の性能チェックに用いる校正標準溶液を確立するために、酸化体と還元体が共存する溶液を校正標準溶液とするために、本発明装置に備えた各収納式の試料槽ＢＯＸ1、試料槽ＢＯＸ２、至る
試料槽ＢＯＸ３で、KCｌ １mol溶液を用いてORP測定精度の比較を試みたのである。その結
果、表1で示す通り、日時が異なる5日間、各5回の測定結果はプラス６０ｍＶを基準にプラスマイナス５ｍＶ以内の再現数値が示され、たまたま偶然に一致したとは考えられず、本発明のORP数値化判定装置および使用方法の校正標準溶液としての所期の目的を達成したのであり、また、唾液ORP測定装置の必須条件である絶対値測定(同一溶液なら、ほぼ同じ値で計測される)とする精度の高い測定装置としての所期の目的を達成したのである。
(2) According to the above (1), using the means for measuring redox potential (ORP) of human saliva with an indicator electrode (platinum) loaded on the bottom of the sample tank, it has already been certified as a medical device by the Ministry of Health, Labor and Welfare. With the cooperation of Dr. Mieko Okazawa, a pediatric physician in Yokohama who proved the physical condition by mV value limitation in saliva clinical practice that exceeds 8,000 cases using the human saliva ORP measuring device. Measurement of saliva ORP, which is the same solution of 20 people including Table 1 shows each storage-type sample tank provided in the apparatus of the present invention in order to establish a calibration standard solution used as a calibration standard solution in order to establish a calibration standard solution used for checking the performance of the ORP electrode. A comparison of ORP measurement accuracy was attempted using a 1 mol KCl solution in the BOX 1, the sample tank BOX 2, and the sample tank BOX 3. As a result, as shown in Table 1, the measurement results of 5 times each for 5 days with different dates and times show a reproducible value within ± 5 mV with reference to plus 60 mV, and it is not considered that it coincided by chance. It achieved the intended purpose as a calibration standard solution for the ORP quantification determination device and method of use of the invention, and was an essential condition of the saliva ORP measurement device (measured with almost the same value for the same solution) The intended purpose as a highly accurate measuring device is achieved.
（３）請求項１至る請求項１０も前記（１）〜（２）と同様に、基準電極、銀―塩化銀電極KCｌ １molを格納する１５ 内部液を格納する液槽の上部蓋となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ１molの溶液を介して連通させるようにしている。
上部蓋となる下面部に細溝を設けたことにより前記の通り基準電極、銀―塩化銀電極KCｌ １molが接触する液絡部と試料槽部の底に内蔵された指示電極の三者間が常に一定の距離を設けられ、測定対象試料とする溶液含浸のみの酸化還元電位ORP値（ｍV）が安定して電子授受交換ができるようにした。本発明の測定装置は、表2に示す通り、収納式の試料槽ＢＯＸ1、試料槽ＢＯＸ２、至る試料槽ＢＯＸ３で、同一検体者の小唾液腺および局所唾液成分が混合された、口腔内の舌の上に綿棒を乗せ口を閉じて採取した総合唾液成分のＯＲＰ値（ｍＶ）の測定値差がプラスマイナス５ｍＶ以内で再現され、偶然に一致したとは考えられず、各収納式の試料槽ＢＯＸを用いた唾液ＯＲＰ測定において、本発明のORP数値化判定装置および使用方法によって、麻薬などの薬物を体内に取り込んだことでの微妙に体内を酸化傾向に導く反応を正確に捉えることを立証するために、唾液の酸化還元電位（ORP）による延べ８,０００症例の数値限定で体内の酸化と還元の度数分布を確立した小児内科医の岡澤美江子医師の測定協力のもと、その実施結果を表１至る表６で後述する。測定対象試料とする麻薬などの薬物を摂取したことによる、唾液ＯＲＰ値（ｍＶ）が酸化有意の強い酸化のストレス状態であるか、又は体内が還元有意の酸化のストレスが無い状態であるか否かのスクリーニング活用を可能にしたのである。
(3) Similarly to the above (1) to (2), in claim 10 leading to claim 1, 15 stores the reference electrode and the silver-silver chloride electrode KCl 1 mol 15 The lower surface serving as the upper lid of the liquid tank storing the internal liquid A narrow groove is provided in the part so that the electronic conduction between both the reference electrode and the indicator electrode is communicated via a KCl 1 mol solution.
By providing a narrow groove in the lower surface part which becomes the upper lid, the reference electrode, the liquid junction where 1 mol of silver-silver chloride electrode KCl 1 mol contacts, and the indicator electrode built in the bottom of the sample tank as described above A constant distance is always provided, and the redox potential ORP value (mV) of only the solution impregnation as a measurement target sample can be stably exchanged. As shown in Table 2, the measuring device of the present invention is a storage-type sample tank BOX1, sample tank BOX2, and sample tank BOX3. The small salivary glands and local saliva components of the same subject are mixed, and the tongue in the oral cavity is mixed. The difference in the ORP value (mV) of the total saliva component collected by placing a cotton swab on top and closing the mouth was reproduced within plus or minus 5 mV, and it was not considered coincidental. In the salivary ORP measurement using an urine, it is proved that the ORP digitization determination device and the method of use of the present invention accurately capture the reaction that subtly causes the body to oxidize due to the incorporation of drugs such as narcotics. Therefore, with the cooperation of measurement by Dr. Mieko Okazawa, a pediatric physician who established the frequency distribution of oxidation and reduction in the body by limiting the numerical value of 8,000 cases by the redox potential (ORP) of saliva. Table 1 to Later in Table 6. Whether or not the saliva ORP value (mV) is due to ingestion of a drug such as narcotics as a measurement target sample, or is in a state where there is no significant oxidation stress in the body This made it possible to use such screening.
又、基準電極、銀―塩化銀電極KCｌ １mol内部液を格納する液槽の上部蓋となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ１molの溶液を介して連通させたことで、より精緻な酸化還元電位測定装置となり、微妙な酸化還元反応を正確にとらえることができ、一例として測定対象試料の応用は、測定対象試料とする綿棒に精製水（純水）を含浸させたＯＲＰ値（ｍＶ）の測定と、同じく綿棒に精製水（純水）を含浸させ、測定場所の空気又は測定対象とする場所の土壌を所定時間触れさせた状態にするなど、多岐にわたって測定対象試料の酸化還元電位の測定が可能となったのである。又、表3至る表６で示す通り、耳下腺左右唾液と舌の上で採取の総体唾液のストレス値差が明確になるORP数値化判定装置および使用方法である。 The reference electrode and silver-silver chloride electrode KCl 1 mol The inner surface of the liquid tank for storing the liquid is provided with a narrow groove on the lower surface, and the reference electrode and the indicator electrode are electrically connected via the KCl 1 mol solution. This makes it possible to obtain a more precise oxidation-reduction potential measurement device and accurately capture subtle oxidation-reduction reactions. As an example, the application of the sample to be measured includes purified water (pure water) Measurement of ORP value (mV) impregnated with water) and impregnation of purified water (pure water) into a cotton swab, and letting the air at the measurement location or the soil at the measurement location touch for a predetermined time, etc. Thus, it has become possible to measure the oxidation-reduction potential of a sample to be measured over a wide range. In addition, as shown in Table 6 leading to Table 3, the ORP quantification determination apparatus and method of use in which the stress value difference between the parotid left and right saliva and the total saliva collected on the tongue is clarified.
本発明のORP数値化判定装置および使用方法を用いてのヒト唾液である同一検体液の
各収納式の試料槽ＢＯＸ１至る試料槽ＢＯＸ３の測定結果。ORP単位（ｍV）。
The measurement result of sample tank BOX3 to each storage-type sample tank BOX1 of the same sample liquid which is human saliva using the ORP digitization determination apparatus and usage method of the present invention. ORP unit (mV).
農薬不使用の植物エキスから抽出の醗酵成分ブレンド壁材を使用して施工した室内での
体感前後のヒト唾液ＯＲＰ経時変化。ORP単位（ｍV）。
Changes in human saliva ORP over time before and after bodily sensation in a room constructed using a fermentation component blend wall material extracted from a plant extract that does not use agricultural chemicals. ORP unit (mV).
シンナー系溶剤を混ぜての壁材を使用して施工した室室内での
体感前後のヒト唾液ＯＲＰ経時変化。ORP単位（ｍV）。
Changes in human saliva ORP over time before and after experiencing in a room constructed using a wall material mixed with a thinner solvent. ORP unit (mV).
無農薬栽培レモンで、保存に防腐剤、カビ防止剤、乾燥防止剤など不使用の
レモン摂取前後の唾液の経時変化。ORP単位（ｍV）。
Changes in saliva over time before and after ingesting lemons that are non-pesticide-grown lemons and are not used for preservatives, fungicides, anti-drying agents, etc. ORP unit (mV).
農薬栽培のレモンで、保存に防腐剤、カビ防止剤、乾燥防止剤などを使用した
レモン摂取前後の唾液の経時変化。ORP単位（ｍV）。
Pesticide-grown lemons used preservatives, fungicides, anti-drying agents, etc. for storage
Changes in saliva over time before and after ingestion of lemon. ORP unit (mV).
表３、表４及び表５、表６について説明する。表３は農薬不使用の植物エキスから抽出の醗酵成分ブレンド壁材部屋での体感前後の唾液の経時変化である。表４はシンナー系使用の室内壁材塗料の部屋での体感前後の唾液の経時変化である。表５は無農薬栽培レモンで、保存に防腐剤、カビ防止剤、乾燥防止剤など不使用のレモン摂取前後の唾液の経時変化である。表６は農薬栽培のレモンで、保存に防腐剤、カビ防止剤、乾燥防止剤などを使用した レモン摂取前後の唾液の経時変化である。この表３至る表６で示されたとおり、住まいの壁材に使用した実例及び果実に使用した実例にあるように、化学物質の体内への影響が、ほぼ同様に酸化反応を示したことで、本発明の本発明のORP数値化判定装置および使用方法が、測定対象試料とする麻薬などの薬物を摂取したことによる、体内が酸化有意の強い酸化状態であるか、又は還元有意の極めて弱い酸化状態か否かのスクリーニング活用が期待できる精度の高い測定装置としての所期の目的を達成したのである。 Table 3, Table 4, Table 5, and Table 6 will be described. Table 3 shows changes over time in saliva before and after experiencing in a fermentation component blend wall material room extracted from a plant extract that does not use agricultural chemicals. Table 4 shows changes with time of saliva before and after the experience in the room of the interior wall material paint using the thinner system. Table 5 shows the time-dependent changes in saliva before and after ingesting lemons that were not used for preservatives, such as preservatives, fungicides, and anti-drying agents. Table 6 shows the changes in saliva over time before and after ingesting lemons, which were preserved with pesticide-grown lemons and used preservatives, fungicides, anti-drying agents, etc. for storage. As shown in Table 6 leading to Table 3, as shown in the examples used for the wall material of the house and the examples used for the fruit, the effects of chemical substances on the body showed an oxidation reaction almost similarly. The ORP quantification determination apparatus and method of use of the present invention are those in which the body is in a highly oxidized state with significant oxidation due to the ingestion of drugs such as narcotics to be measured, or the reduction is extremely weak. This achieves the intended purpose of a highly accurate measuring device that can be expected to be used for screening whether it is in an oxidized state or not.
本発明者は麻薬と呼ばれる物の中には覚醒剤、大麻、アヘン、などの常用者の唾液臨床ができない状況から、唾液の酸化還元電位（ORP）による延べ８,０００症例の数値限定で体内の酸化と還元の度数分布を確立した小児内科医の岡澤美江子医師の測定協力のもと、表３至る表６に見られるように化学物質によって、人間の生体反応は、明確に唾液成分が強い酸化反応を示したのである。すなわち、化学物質により体内は大量に活性酸素が発生していることは明白であり、よって、表３至る表６で示したとおり人間の唾液ORPの酸化反応の経時変化が偶然に一致したとは考えられず、シンナー系の化学物質を使用した室内空気の体内摂取と農薬などの化学物質が含まれた果実の体内摂取において、本発明である基準電極、銀―塩化銀電極KCｌ １molの内部液を格納する液槽の上部蓋となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ１molの溶液を介して連通させるようにして、電子移動をスムースにできると考察した結果は、前述表1至る表6で示した通りである。 Since the present inventor cannot perform saliva clinical trials for regular users such as stimulants, cannabis, opium, etc. among the so-called narcotics, the number of cases in the body is limited to a total of 8,000 cases due to the redox potential (ORP) of saliva. Under the cooperation of measurement by Dr. Mieda Okazawa, a pediatric physician who has established the frequency distribution of oxidation and reduction, human biological reactions are clearly strong in salivary components due to chemical substances as shown in Table 3 to Table 6. It showed an oxidation reaction. In other words, it is clear that active oxygen is generated in large quantities in the body due to chemical substances. Therefore, as shown in Table 6 to Table 3, the time-dependent changes in human salivary ORP oxidation coincided by chance. Unexpectedly, in the intake of indoor air using thinner chemicals and the intake of fruits containing chemicals such as agricultural chemicals, the internal solution of the reference electrode, 1 mol of silver-silver chloride electrode KCl, which is the present invention When a thin groove is provided in the lower surface part that serves as the upper lid of the liquid tank for storing the reference electrode and the indicator electrode, the electronic conduction between the two electrodes is communicated via a KCl 1 mol solution, thereby smoothing the electron transfer. The result of consideration is as shown in Table 6 leading to Table 1 above.
被験者の唾液を含浸させた綿棒を差し込む試料槽部を設けたORP数値化判定装置および使用方法である。請求項に述べている、麻薬と呼ばれる物の中には覚醒剤、大麻、アヘン、モルヒネ、ヘロイン、MDMA・MDA、コカイン、シンナー等有機溶剤、危険ドラッグなどがあり、これらの摂取乱用により、体内細胞への酸化反応が強くなる。大麻などの植物による神経細胞の麻痺は幻覚症状であり、正常な生体機能をクラッシュさせ酸化となる。さらに、覚せい剤は人工の化学物質を混ぜたメタンフェタミンであり、これらの人工的な化学物質を体内に取り込むことで、体内は大量の強い活性酸素が生成されることが考察されるとする理由を表３至る表６で証明したのであり、血液を通して唾液の酸化還元反応が、飲食又は体感後において最速で３０分経過後に色濃く現れる生体反応の特性を捉え、覚せい剤反応のスクリーニング活用が大いに期待できることに着眼したのである。 It is an ORP digitization determination apparatus provided with a sample tank portion into which a cotton swab impregnated with saliva of a subject is inserted and a method of use. Some of the so-called narcotics mentioned in the claims include stimulants, cannabis, opium, morphine, heroin, MDMA / MDA, cocaine, thinner, and other organic solvents, dangerous drugs, and so on. The oxidation reaction to becomes stronger. The paralysis of nerve cells by plants such as cannabis is a hallucinatory symptom, which crashes normal biological functions and becomes oxidized. Furthermore, the stimulant is methamphetamine mixed with artificial chemicals, and it is considered that a large amount of strong active oxygen is generated in the body by taking these artificial chemicals into the body. As proved in Table 6 through Table 3, the saliva redox reaction through the blood captures the characteristics of the biological reaction that appears dark after 30 minutes at the fastest after eating, drinking, or bodily sensation, and the screening utilization of the stimulant reaction can be greatly expected I focused on.
図１で示す通り、基準電極、銀―塩化銀電極KCｌ １molが接触する液絡部と試料槽部の底に内蔵された指示電極の三者間が常に一定の距離を設けられ、測定中に指示電極が溶液含浸綿棒に覆われることで空気に触れて酸化還元の緩衝を受けずに、測定対象試料とする溶液含浸のみの酸化還元電位ORP値（ｍV）が安定して電子授受交換ができるようにしているのである。本発明に測定装置に用いる内部液の濃度は、KCｌ １molに限定されない、 As shown in Fig. 1, there is always a fixed distance between the reference electrode, the liquid junction where 1 mol of silver-silver chloride electrode KCl contacts, and the indicator electrode built in the bottom of the sample tank. Since the indicator electrode is covered with a solution-impregnated cotton swab, the oxidation-reduction potential ORP value (mV) of only the solution impregnation used as a measurement target sample can be stably exchanged without touching the air and receiving buffer for oxidation-reduction. It is doing so. The concentration of the internal liquid used in the measuring apparatus according to the present invention is not limited to 1 mol KCl.
つぎに、本発明の図２で示す通り、基準電極、銀―塩化銀電極KCｌ １molを格納する１５ 内部液を格納する液槽の上部蓋となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ １molの溶液を介して連通させるようにした構成図である。これは浴槽室内において天井の溝に水滴が足る速い動きからの着眼で、内部液を格納する液槽の上部蓋となる下面部に細溝を設けたことは浴槽室内の天井に溝をほどこしたことと同じになり、液絡部からのKCｌ １molの流出で起きる結晶化が極少にでき、唾液含浸の綿棒を測定の試料槽部に差し込むことで毛細管現象を利用してのKCｌ １mol 溶液の流出が最小で抑えられ、測定対象試料であるプラスマイナスの電子移動速度が速くなり、安定した再現精度測定は、表１及び表２で示されたとおりである。 Next, as shown in FIG. 2 of the present invention, a reference electrode, a silver-silver chloride electrode KCl 1 containing 15 mol, a narrow groove is provided in the lower surface portion serving as an upper lid of the liquid tank containing the internal liquid, and the reference electrode and instructions It is the block diagram which made the electronic conduction of the both electrodes which are electrodes communicate through the solution of 1 mol of KCl. This is because of the quick movement of water droplets in the ceiling groove in the bathtub room, and the provision of a narrow groove on the bottom surface, which is the upper lid of the liquid tank that stores the internal liquid, gave the groove to the ceiling in the bathtub room. In the same way, the crystallization caused by the outflow of 1 mol of KCl from the liquid junction can be minimized, and the outflow of the 1 mol solution of KCl using capillary action by inserting a saliva-impregnated cotton swab into the sample tank for measurement. Is suppressed to a minimum, the plus / minus electron transfer speed of the sample to be measured is increased, and stable reproducibility measurement is as shown in Tables 1 and 2.
本発明を実施するための最良の形態について説明する。内部液を格納する液槽の上部蓋
となる下面部に細溝を設け、基準電極と指示電極である両電極の電子導通をKCｌ １molの溶液を介して連通させるようにしたORP数値化判定装置および使用方法である。唾液の生体反応は酸化体と還元体の電子の授受であり、電子の流れは負極（マイナス）から正極（プラス）となり、マイナス電子を奪われ酸化することを意味する。負極（マイナス）電子が放出（奪われた）場合は酸化反応であり、反対に起こっている正極（プラス）が負極（マイナス）電子を受け取る場合は還元反応である。人間の体は常に負極（マイナス）電子が奪われる自然摂理で構成され、酸化反応をいかに抑えるかが健康で生きるための大切な要件である。
The best mode for carrying out the present invention will be described. An ORP quantification judgment device that has a narrow groove in the lower surface part that serves as the upper lid of the liquid tank that stores the internal liquid, and allows the electronic conduction of both the reference electrode and the indicator electrode to communicate with each other via a 1 mol solution of KCl. And how to use. The biological reaction of saliva is the exchange of electrons between an oxidant and a reductant, and the flow of electrons changes from the negative electrode (minus) to the positive electrode (plus), meaning that negative electrons are taken away and oxidized. When the negative electrode (minus) electron is released (stolen), it is an oxidation reaction, and when the positive electrode (plus) which is taking place on the contrary receives the negative electrode (minus) electron, it is a reduction reaction. The human body is always made up of natural providence where negative (negative) electrons are taken away, and how to suppress the oxidation reaction is an important requirement for a healthy life.
図１について説明する。８は本発明のORP数値化判定装置および使用方法において、測
定装置の試料槽の底部に装填の指示電極と基準電極の内部液格納の溶液を介しての電子
授受の構成の断面図であり、９は酸化還元電位差計を有し、１０は指示電極と参照電極導通のリード線であり、１１は参照電極となる銀電極とし、１２は試料槽部土台、１３は試料槽部上面、１４試料槽部上部浅瀬部分であり、１５は内部液を格納する液槽の上部ふたを設け、１６は内部液を格納する液槽の上面であり、１７には塩化カリウムが格納されてる。１８は基準電極の銀線ＫＣl １mol内部液槽の蓋となる、下面部に細溝が連通し、１９は銀線が内蔵され、
２０は銀線を包み込む銀粉であり、２１は内部液を格納する液槽のKCｌ溶液が漏れないようにパッキンを挟み込むようにしている。２２はガラスやカーボン等の不活性素材からなる筒部を設け、２３はコットンを詰め込み銀粉が流出しないようにしている。２４は綿棒などの多孔性物質を差込む縦筒穴の底部であり接着ボンドで４の白金電極を固定している。２５は指示電極棒状の下部をナットで締めるようにしてゆるみが出ないようにしている。２６は試料槽部に差し込む綿棒などの多孔性物質で構成される持ち手部が木軸及び紙軸であり、２７は綿棒の綿部分に含浸の被検液とKCｌ １mol溶液が接触する液絡部であり、２８は指示電極と綿棒に含浸の唾液とKCｌ １mol溶液が接する液絡部への導通イメージであり、２９は指示電極の頭部を丸み形状をもたせ唾液含浸綿棒との接触部で構成している。
With reference to FIG. 8 is a cross-sectional view of the configuration of electron exchange through the solution stored in the internal liquid of the indicator electrode and the reference electrode loaded in the bottom of the sample tank of the measuring device in the ORP digitization determination apparatus and method of use of the present invention; 9 is an oxidation-reduction potentiometer, 10 is a lead wire for conducting the indicator electrode and the reference electrode, 11 is a silver electrode serving as a reference electrode, 12 is a base for the sample tank, 13 is the top of the sample tank, and 14 samples An upper shallow portion of the tank portion, 15 is provided with an upper lid of the liquid tank for storing the internal liquid, 16 is an upper surface of the liquid tank for storing the internal liquid, and 17 stores potassium chloride. 18 is a lid of a 1 mol silver wire KCl 1 mol internal liquid tank of the reference electrode, and a narrow groove communicates with the lower surface portion, 19 is a silver wire built-in,
Reference numeral 20 denotes a silver powder that encloses the silver wire, and reference numeral 21 denotes a packing so that the KCl solution in the liquid tank that stores the internal liquid does not leak. 22 is provided with a cylindrical portion made of an inert material such as glass or carbon, and 23 is filled with cotton so that silver powder does not flow out. Reference numeral 24 denotes a bottom portion of a vertical cylindrical hole into which a porous material such as a cotton swab is inserted, and 4 platinum electrodes are fixed by an adhesive bond. No. 25 prevents the looseness from occurring by tightening the lower part of the indicator electrode bar with a nut. Reference numeral 26 is a handle made of a porous material such as a cotton swab inserted into the sample tank, and a wooden shaft and a paper shaft are provided. 27 is a liquid junction where the test solution impregnated with the cotton portion of the cotton swab is in contact with the 1 mol KCl solution 28 is an image of conduction to the liquid junction where the saliva impregnated on the indicator electrode and the cotton swab is in contact with the 1 mol solution of KCl, and 29 is the contact portion with the saliva-impregnated cotton swab by rounding the head of the indicator electrode. It is composed.
図２について説明する。図２において８の本発明の１０は指示電極と参照電極導通のリード線であり、１１は参照電極となる銀電極とし、１５は内部液を格納する液槽の上部ふたを設け、１６は内部液を格納する液槽の上面である。１８は基準電極の銀線ＫＣＬ溶液導通の細溝とし、内部液を格納する液槽の上部ふたの下面部に細溝を設けることで測定対象試料の電子導通が加速され安定した酸化還元電位測定が得られることを前記表１及び表2で示した通りである。 With reference to FIG. In FIG. 2, 10 of the present invention 8 is a lead wire for conducting the indicator electrode and the reference electrode, 11 is a silver electrode as a reference electrode, 15 is provided with an upper lid of a liquid tank for storing the internal liquid, and 16 is an internal electrode. It is the upper surface of the liquid tank which stores a liquid. Reference numeral 18 is a narrow groove for conducting the silver wire KCL solution of the reference electrode, and by providing a narrow groove on the lower surface of the upper lid of the liquid tank for storing the internal liquid, the electronic conduction of the sample to be measured is accelerated and stable redox potential measurement. As shown in Table 1 and Table 2 above.
本発明の測定装置は、1台の装置で、舌の上に集まる総合唾液および還元有意に導くアミラーゼ、パロチン、ラクトペルオキシターゼ、ヒスタチンなどを主流とする酸化影響を受けやすい耳下腺左右から分泌される唾液成分3か所のヒト唾液成分の酸化還元電位値（ｍＶ）比較測定を可能にし、２０２０年のオリンピックにおいてのスクリーニング検査。各国選手は国民性の違いから下着を脱いでのドーピング検査に抵抗があり、検査時間がかかることから、唾液による簡便な行為でスクリーニング検査活用で、疑わしき場合には、尿、血液検査など絞り込んだ検査体制に進ませることが可能となり、早期の覚せい剤乱用阻止と一般市民の社会生活、集団組織の規律を遵守することなど産業上での利用は大いに期待できるのである。
The measuring device of the present invention is a single device that is secreted from the left and right of the parotid gland, which is susceptible to oxidative effects such as total saliva collected on the tongue and amylase, parotin, lactoperoxidase, histatin, etc. This enables screening of redox potential (mV) of human saliva components in three locations, and screening tests at the 2020 Olympics. Players from each country are resistant to doping tests after taking off their underwear due to differences in national character, and it takes a long time to test. By using a screening test with a simple act of saliva, in case of doubt, we narrowed down urine and blood tests. It is possible to proceed to the inspection system, and industrial use such as prevention of stimulant drug abuse at an early stage, social life of the general public, and compliance with group organization regulations can be greatly expected.
８ 試料槽の指示電極と基準電極の内部液格納の溶液を介しての電子授受の構成の断面図
９ 酸化還元電位差計
１０ 指示電極と参照電極導通のリード線
１１ 参照電極となる銀電極
１２ 試料槽部土台
１３ 試料槽部上面
１４ 試料槽部上部浅瀬部分
１５ 内部液を格納する液槽の上部ふた面
１６ 内部液を格納する液槽の上面
１７ 塩化カリウム
１８ 基準電極の銀線ＫＣl １mol内部液槽の蓋となる、下面部に細溝が連通
１９ 銀線
２０ 銀線を包み込む銀粉
２１ 内部液を格納する液槽のＫＣl １mol溶液が漏れないようにパッキンを挟み込む
２２ ガラスやカーボン等の不活性素材からなる筒部
２３ コットン
２４ 綿棒などの多孔性物質を差込む縦筒穴の底部は接着ボンドで白金電極を固定
２５ 指示電極棒状の下部をナットで試料槽の底部に固定の白金電極を締める
２６ 試料槽部に差し込む綿棒などの多孔性物質で構成される持ち手部が木軸及び紙軸
２７ 綿棒の綿部分に含浸の被検液とＫＣl １mol溶液が接触する液絡部
２８ 指示電極と綿棒に含浸された唾液溶液とＫＣl １mol溶液が接触する液絡部への導通部
２９ 指示電極先端部を丸み形状として唾液含浸綿棒との接触部
8 Cross-sectional view of the configuration of electron exchange through the solution stored in the internal solution of the indicator electrode and the reference electrode of the sample tank 9 Redox potentiometer 10 Lead wire for indicating electrode and reference electrode conduction 11 Silver electrode 12 serving as reference electrode Sample Tank base 13 Sample tank upper surface 14 Sample tank upper shallow part 15 Upper lid surface of the liquid tank for storing the internal liquid 16 Upper surface of the liquid tank for storing the internal liquid 17 Potassium chloride 18 Silver wire KCl 1 mol of the reference electrode internal liquid A narrow groove communicates with the bottom of the tank, which is a lid for the tank. 19 Silver wire 20 Silver powder that wraps the silver wire 21 Packs the packing so that the 1 mol solution of KCl in the liquid tank that contains the internal liquid does not leak 22 Inactive glass or carbon Tube made of material
23 Cotton 24 The bottom of the vertical cylindrical hole into which a porous material such as a cotton swab is inserted is fixed with an adhesive bond to fix the platinum electrode. 25 The lower part of the indicator electrode bar is fastened to the bottom of the sample tank with a nut. The handle composed of a porous material such as a cotton swab is inserted into a wooden shaft and a paper shaft. 27 The liquid junction portion where the test liquid impregnated with the cotton portion of the cotton swab contacts the 1 mol solution of KCl.
28 Conductive part to liquid junction where saliva solution impregnated with indicator electrode and cotton swab contacts with KCl 1 mol solution 29 Contact part with saliva-impregnated cotton swab with rounded tip of indicator electrode
Claims (10)
格納する内部液槽の蓋となる、下面部に細溝が設けられ、
前記参照電極である銀―塩化銀電極と測定対象試料を含浸させた綿棒と接する液絡部、
前記測定対象試料が接触する指示電極の三者間が、
内部液を保持する液絡部と細溝で電子導通され、
参照電極と指示電極が接続される酸化還元電位差計を備えており、
測定中に、前記指示電極が直接、空気に触れずに、
前記内部液槽の蓋となる、下面部に細溝が連通する、
試料槽部に測定対象試料を差し込むことで、
測定対象試料の酸化還元電位（ｍＶ）の測定値を、
比較検証することを特徴とするＯＲＰ数値化判定測定装置。 Holds the internal solution of the reference electrode and silver-silver chloride electrode,
A narrow groove is provided on the lower surface, which serves as a lid for the internal liquid tank to be stored,
A liquid junction that contacts the silver-silver chloride electrode as the reference electrode and a cotton swab impregnated with the sample to be measured;
Between the three of the indicator electrodes in contact with the sample to be measured,
Electronic conduction is made between the liquid junction that holds the internal liquid and the narrow groove,
It has a redox potentiometer to which the reference electrode and the indicator electrode are connected,
During the measurement, the indicator electrode does not touch the air directly,
A narrow groove communicates with the lower surface portion, which serves as a lid for the internal liquid tank.
By inserting the sample to be measured into the sample tank,
The measured value of the oxidation-reduction potential (mV) of the sample to be measured is
An ORP digitization determination measurement apparatus characterized by comparing and verifying.
同じく小唾液腺および局所唾液成分が混在している、
口腔内の舌の上に綿棒を乗せ採取した総合唾液成分の３か所の、
ヒト唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）との値差を比較するのであり、
前記内部液槽の蓋となる、下面部に細溝が連通され、
前記ORP数値化判定装置に複数、又は単独で設けられた試料槽部に、
測定対象試料を差し込むことで、
測定対象試料とする麻薬などの薬物を摂取したことによる、
唾液ＯＲＰ値（ｍＶ）が酸化有意の強い酸化のストレス状態であるか、
又は薬物を摂取などの無い、還元有意で酸化のストレスが、
無い状態であるか否かを、見極めるスクリーニング活用ができるようにし、
測定対象試料の酸化還元電位（ｍＶ）の測定値を、
比較検証することを特徴とするORP数値化判定測定装置。 Measurement of the ORP value (mV) of the local salivary component that is the left and right parotid gland of the same subject,
Similarly, small salivary glands and local saliva components are mixed,
Three points of total saliva components collected by placing a cotton swab on the tongue in the oral cavity,
Compare the difference between the redox potential (ORP) value (mV) of human saliva,
A narrow groove is communicated with the lower surface portion, which becomes a lid of the internal liquid tank,
In the ORB quantification determination apparatus, a plurality of or a sample tank unit provided alone,
By inserting the sample to be measured,
By taking drugs such as narcotics to be measured samples,
Whether the saliva ORP value (mV) is an oxidative significant strong oxidative stress state,
Or, there is no reduction or significant oxidative stress without taking any drugs.
So that screening can be used to determine whether or not there is
The measured value of the oxidation-reduction potential (mV) of the sample to be measured is
ORP digitization determination measurement device characterized by comparative verification.
同じく小唾液腺および局所唾液成分が混在している、
口腔内の舌の上に綿棒を乗せ採取した総合唾液成分の
ヒト唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）との値差を比較するのであり、
前記内部液槽の蓋となる、下面部に細溝が連通され、
前記ORP数値化判定装置に複数、又は単独で設けられた試料槽部に、
測定対象試料を差し込むことで、
測定対象試料とする麻薬などの薬物を摂取したことによる、
前記耳下腺左右唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）が、
プラス１ｍＶ以上からプラス７０ｍＶ方向の範囲において、
酸化有意の弱い酸化のストレス状態であるか、
又は前記耳下腺左右唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）が、
プラス７１ｍＶ以上からプラス２５０ｍＶ方向の範囲において、
酸化有意の強い酸化のストレス状態であるか否かを、
見極めるスクリーニング活用ができるようにし、
請求項１又は２に記載のORP数値化判定装置で
測定値を比較検証することを特徴とする使用方法。 Measurement of the ORP value (mV) of the local salivary component that is the left and right parotid gland of the same subject,
Similarly, small salivary glands and local saliva components are mixed,
Compare the difference between the total saliva component collected by placing a cotton swab on the tongue in the oral cavity and the redox potential (ORP) value (mV) of human saliva,
A narrow groove is communicated with the lower surface portion, which becomes a lid of the internal liquid tank,
In the ORB quantification determination apparatus, a plurality of or a sample tank unit provided alone,
By inserting the sample to be measured,
By taking drugs such as narcotics to be measured samples,
The parotid gland saliva redox potential (ORP) value (mV)
In the range from plus 1 mV or more to plus 70 mV,
Oxidation is a significant weak oxidative stress state,
Or the redox potential (ORP) value (mV) of the parotid gland saliva
In the range from plus 71 mV or more to plus 250 mV,
Whether or not it is a strong oxidative stress state,
So that screening can be used to identify
A method of use, comprising: comparing and verifying measured values with the ORP digitization determination apparatus according to claim 1.
同じく小唾液腺および局所唾液成分が混在している、
口腔内の舌の上に綿棒を乗せ採取した総合唾液成分の
ヒト唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）との値差を比較するのであり、
前記内部液槽の蓋となる、下面部に細溝が連通され、
前記ORP数値化判定装置に複数、又は単独で設けられた試料槽部に、
測定対象試料を差し込むことで、
測定対象試料とする麻薬などの薬物を摂取したことによる、
前記舌の上で採取した総合唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）が、
プラス４１ｍＶ以上からプラス１１０ｍＶ方向の範囲において、
酸化有意の弱い酸化のストレス状態であるか、
又は前記舌の上で採取した総合唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）が、
プラス１１１ｍＶ以上からプラス２５０ｍＶ方向の範囲において、
酸化有意の強い酸化のストレス状態であるか否かを、
見極めるスクリーニング活用ができるようにし、
請求項１又は２に記載のORP数値化判定装置で
測定値を比較検証することを特徴とする使用方法。 Measurement of the ORP value (mV) of the local salivary component that is the left and right parotid gland of the same subject,
Similarly, small salivary glands and local saliva components are mixed,
Compare the difference between the total saliva component collected by placing a cotton swab on the tongue in the oral cavity and the redox potential (ORP) value (mV) of human saliva,
A narrow groove is communicated with the lower surface portion, which becomes a lid of the internal liquid tank,
In the ORB quantification determination apparatus, a plurality of or a sample tank unit provided alone,
By inserting the sample to be measured,
By taking drugs such as narcotics to be measured samples,
The redox potential (ORP) value (mV) of total saliva collected on the tongue is
In the range from +41 mV to +110 mV,
Oxidation is a significant weak oxidative stress state,
Or the redox potential (ORP) value (mV) of the total saliva collected on the tongue,
In the range from plus 111 mV to plus 250 mV,
Whether or not it is a strong oxidative stress state,
So that screening can be used to identify
A method of use, comprising: comparing and verifying measured values with the ORP digitization determination apparatus according to claim 1.
同じく小唾液腺および局所唾液成分が混在している、
口腔内の舌の上に綿棒を乗せ採取した総合唾液成分の
ヒト唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）との値差を比較するのであり、
前記内部液槽の蓋となる、下面部に細溝が連通され、
前記ORP数値化判定装置に複数、又は単独で設けられた試料槽部に、
測定対象試料を差し込むことで、
測定対象試料とする麻薬などの薬物を摂取したことによる、
前記耳下腺左右唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）が、
プラスマイナス０ｍＶ以下からマイナス３０ｍＶ方向の範囲において、
還元有意の酸化のストレスが極めて弱い状態であるか、
又は前記耳下腺左右唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）が、
マイナス３１ｍＶ以下からマイナス２５０ｍＶ方向の範囲において、
還元有意の酸化のストレスが無い状態であるか否かを、
見極めるスクリーニング活用ができるようにし、
請求項１、２又は３に記載のORP数値化判定装置で
測定値を比較検証することを特徴とする使用方法。 Measurement of the ORP value (mV) of the local salivary component that is the left and right parotid gland of the same subject,
Similarly, small salivary glands and local saliva components are mixed,
Compare the difference between the total saliva component collected by placing a cotton swab on the tongue in the oral cavity and the redox potential (ORP) value (mV) of human saliva,
A narrow groove is communicated with the lower surface portion, which becomes a lid of the internal liquid tank,
In the ORB quantification determination apparatus, a plurality of or a sample tank unit provided alone,
By inserting the sample to be measured,
By taking drugs such as narcotics to be measured samples,
The parotid gland saliva redox potential (ORP) value (mV)
In the range from plus or minus 0 mV or less to minus 30 mV,
Reduction significant oxidative stress is very weak,
Or the redox potential (ORP) value (mV) of the parotid gland saliva
In the range from minus 31 mV or less to minus 250 mV,
Whether or not there is no significant oxidative stress,
So that screening can be used to identify
4. A method of use comprising comparing and verifying measured values with the ORP digitization determination apparatus according to claim 1.
同じく小唾液腺および局所唾液成分が混在している、
口腔内の舌の上に綿棒を乗せ採取した総合唾液成分の
ヒト唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）との値差を比較するのであり、
前記内部液槽の蓋となる、下面部に細溝が連通され、
前記ORP数値化判定装置に複数、又は単独で設けられた試料槽部に、
測定対象試料を差し込むことで、
測定対象試料とする麻薬などの薬物を摂取したことによる、
前記舌の上で採取した総合唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）が、
プラス４０ｍＶ以下からマイナス１０ｍＶ方向の範囲において、
還元有意の酸化のストレスが極めて弱い状態であるか
又は前記舌の上で採取した総合唾液の酸化還元電位（ＯＲＰ）値（ｍＶ）が、
マイナス１１ｍＶ以下からマイナス２５０ｍＶ方向の範囲において、
還元有意の酸化のストレスが無い状態であるか否かを、
見極めるスクリーニング活用ができるようにし、
請求項１、２又は４に記載のORP数値化判定装置で、
測定値を比較検証することを特徴とする使用方法。 Measurement of the ORP value (mV) of the local salivary component that is the left and right parotid gland of the same subject,
Similarly, small salivary glands and local saliva components are mixed,
Compare the difference between the total saliva component collected by placing a cotton swab on the tongue in the oral cavity and the redox potential (ORP) value (mV) of human saliva,
A narrow groove is communicated with the lower surface portion, which becomes a lid of the internal liquid tank,
In the ORB quantification determination apparatus, a plurality of or a sample tank unit provided alone,
By inserting the sample to be measured,
By taking drugs such as narcotics to be measured samples,
The redox potential (ORP) value (mV) of total saliva collected on the tongue is
In the range from plus 40 mV or less to minus 10 mV,
Reduction Significant oxidative stress is very weak or the total saliva redox potential (ORP) value (mV) collected on the tongue is
In the range from minus 11 mV or less to minus 250 mV,
Whether or not there is no significant oxidative stress,
So that screening can be used to identify
In the ORP quantification judging device according to claim 1, 2, or 4,
A method of use characterized by comparing and verifying measured values.
格納する内部液槽の蓋となる、下面部に細溝が設けられ、
前記参照電極である銀―塩化銀電極と測定対象試料を含浸させた綿棒と接する液絡部、
前記測定対象試料が接触する指示電極の三者間が、
内部液を保持する液絡部と細溝で電子導通され、
前記ORP数値化判定装置に複数、又は単独で設けられた試料槽部に、
校正標準溶液とするＫＣl １mol溶液を測定対象試料として差し込むことで、
酸化還元電位測定装置の電極の校正及び性能チェックに活用できるようにし、
請求項１又は２に記載のORP数値化判定装置で、
測定値の再現精度を比較検証することを特徴とする使用方法。 Hold the internal solution of the reference electrode, silver-silver chloride electrode,
A narrow groove is provided on the lower surface, which serves as a lid for the internal liquid tank to be stored,
A liquid junction that contacts the silver-silver chloride electrode as the reference electrode and a cotton swab impregnated with the sample to be measured;
Between the three of the indicator electrodes in contact with the sample to be measured,
Electronic conduction is made between the liquid junction that holds the internal liquid and the narrow groove,
In the ORB quantification determination apparatus, a plurality of or a sample tank unit provided alone,
By inserting a 1 mol solution of KCl as a calibration standard solution as a sample to be measured,
It can be used for the calibration and performance check of the electrode of the oxidation-reduction potential measuring device,
In the ORP numerical determination apparatus according to claim 1 or 2,
A method of use characterized by comparing and verifying the reproducibility of measured values.
格納する内部液槽の蓋となる、下面部に細溝が設けられ、
前記参照電極である銀―塩化銀電極と測定対象試料を含浸させた綿棒と接する液絡部、
前記測定対象試料が接触する指示電極の三者間が、
内部液を保持する液絡部と細溝で電子導通され、
前記ORP数値化判定装置に複数、又は単独で設けられた試料槽部に、
校正標準溶液とするＫＣl １mol溶液を測定対象試料として差し込むことで、
酸化還元電位測定装置の測定動作の校正及び性能チェックに活用できるようにし、
請求項１又は２に記載のORP数値化判定装置で、
測定値の再現精度を比較検証することを特徴とする使用方法。 Hold the internal solution of the reference electrode, silver-silver chloride electrode,
A narrow groove is provided on the lower surface, which serves as a lid for the internal liquid tank to be stored,
A liquid junction that contacts the silver-silver chloride electrode as the reference electrode and a cotton swab impregnated with the sample to be measured;
Between the three of the indicator electrodes in contact with the sample to be measured,
Electronic conduction is made between the liquid junction that holds the internal liquid and the narrow groove,
In the ORB quantification determination apparatus, a plurality of or a sample tank unit provided alone,
By inserting a 1 mol solution of KCl as a calibration standard solution as a sample to be measured,
It can be used for calibration and performance check of the measurement operation of the oxidation-reduction potential measuring device,
In the ORP numerical determination apparatus according to claim 1 or 2,
A method of use characterized by comparing and verifying the reproducibility of measured values.
同じく綿棒に精製水（純水）を含浸させ、測定場所の空気を所定時間
触れさせた状態での測定対象試料とした、
酸化還元電位（ＯＲＰ）値（ｍＶ）との測定値差および経時変化の値差を
同時に測定し、比較するのであり、
前記内部液槽の蓋となる、下面部に細溝が連通され、
前記ORP数値化判定装置に複数、又は単独で設けられた試料槽部に、
前記測定対象試料を、それぞれ差し込むことで、
請求項１に記載の数値化判定装置で、
複数同時、又は単独で測定値を比較検証することを特徴とする使用方法。 Measurement of ORP value (mV) by impregnating purified water (pure water) into a cotton swab to be measured;
Similarly, a cotton swab was impregnated with purified water (pure water) and used as a sample to be measured in a state where the measurement place air was touched for a predetermined time.
The measured value difference with the oxidation-reduction potential (ORP) value (mV) and the value difference with time are simultaneously measured and compared.
A narrow groove is communicated with the lower surface portion, which becomes a lid of the internal liquid tank,
In the ORB quantification determination apparatus, a plurality of or a sample tank unit provided alone,
By inserting each of the measurement target samples,
The numerical determination apparatus according to claim 1,
A method of use characterized by comparing and verifying a plurality of measured values simultaneously or independently.
同じく綿棒に精製水（純水）を含浸させ、測定場所の土壌を所定時間
触れさせた状態での測定対象試料とした、
酸化還元電位（ＯＲＰ）値（ｍＶ）との測定値差および経時変化の値差を
同時に測定し、比較するのであり、
前記内部液槽の蓋となる、下面部に細溝が連通され、
酸化還元電位測定装置に複数で設けられた試料槽部に、
前記測定対象試料を、それぞれ差し込むことで、
請求項１に記載の数値化判定装置で、
複数同時、又は単独で測定値を比較検証することを特徴とする使用方法。
Measurement of ORP value (mV) by impregnating purified water (pure water) into a cotton swab to be measured;
Similarly, a cotton swab was impregnated with purified water (pure water) and used as a sample to be measured in a state where the soil at the measurement location was touched for a predetermined time.
The measured value difference with the oxidation-reduction potential (ORP) value (mV) and the value difference with time are simultaneously measured and compared.
A narrow groove is communicated with the lower surface portion, which becomes a lid of the internal liquid tank,
A plurality of sample tanks provided in the oxidation-reduction potential measuring device,
By inserting each of the measurement target samples,
The numerical determination apparatus according to claim 1,
A method of use characterized by comparing and verifying a plurality of measured values simultaneously or independently.
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| JP7336166B1 (en) * | 2023-05-16 | 2023-08-31 | 慶孝 大友 | ORP measuring device using pure gold as working electrode |
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| CN114778648B (en) * | 2022-04-24 | 2023-10-31 | 深圳科瑞德健康科技有限公司 | System and method for testing oxidation-reduction potential value of aqueous solution |
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