JP2019058094A - Collagen-blended beverage and method for producing the same - Google Patents
Collagen-blended beverage and method for producing the same Download PDFInfo
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Landscapes
- Tea And Coffee (AREA)
Abstract
Description
本発明は、コラーゲンが配合されてなる発酵茶飲料またはコーヒー飲料とその製造方法に関する。 The present invention relates to a fermented tea beverage or coffee beverage containing collagen and a method for producing the same.
近年の口腔審美に対する意識の高まりから、歯の着色汚れであるステインは審美上の主要な課題となっている。ステインは、食品中に含まれるポリフェノール、クルクミン、アミノカルボニル反応物等の着色物質や、煙草のタール(ヤニ)等の着色物質と、歯のエナメル質表面を覆う唾液成分ペリクルが結合して歯に付着することで生じることが知られている。 With the recent increase in awareness of oral esthetics, stains, which are stains on teeth, have become a major cosmetic issue. Stain consists of coloring substances such as polyphenols, curcumin and aminocarbonyl reactant contained in food, coloring substances such as cigarette tar (Jani), and saliva component pellicle covering the enamel surface of teeth, so that teeth are attached to teeth. It is known that it arises by adhering.
歯に付着したステインの除去を目的とした様々な手法が開発・検討されてきている。例えば、特許文献1には、ポリリン酸塩など4成分を含有するステイン除去効果の高い歯磨剤組成物が開示されている。特許文献2には、メタリン酸ナトリウムを含有するステイン除去用口腔用組成物が開示されている。しかし、これらの技術は、歯牙に付着したステインの除去を目的とした技術であり、歯牙へのステイン付着の抑制を目的とした食品はこれまで知られていない。 Various methods for removing stains attached to teeth have been developed and studied. For example, Patent Document 1 discloses a dentifrice composition having a high stain removing effect containing four components such as polyphosphate. Patent Document 2 discloses a stain removal oral composition containing sodium metaphosphate. However, these techniques are techniques aimed at the removal of stain attached to teeth, and food intended to suppress stain attachment to teeth has not been known so far.
本発明は、それ自身に含まれるポリフェノールに起因する歯牙へのステイン付着と、煙草の吸引および/またはステイン付着性食品の摂取に起因する歯牙へのステイン付着とを抑制することができる発酵茶飲料およびコーヒー飲料とその製造方法の提供を目的とする。 The present invention is a fermented tea beverage capable of suppressing stain adhesion to teeth due to polyphenol contained in itself and stain adhesion to teeth due to intake of tobacco and / or stain-adherent food. And it aims at provision of a coffee drink and its manufacturing method.
本発明者らは今般、紅茶飲料と一緒に煙草を吸引したり、ステイン付着性食品を摂取したりすると、歯牙へのステイン付着がより強まること、そして、紅茶飲料摂取時の煙草やステイン付着性食品に起因するステイン付着を所定濃度のコラーゲンが効果的に抑制することを見出した。本発明はこれらの知見に基づくものである。 The present inventors now intensify the adhesion of stains to teeth when sucking tobacco or take stain-adherent food along with black-tea drinks, and the tobacco and stain-adhesion when black tea drinks are ingested. It has been found that collagen of a predetermined concentration effectively suppresses stain adhesion caused by food. The present invention is based on these findings.
本発明によれば以下の発明が提供される。
[1]コラーゲンが配合されてなる発酵茶飲料またはコーヒー飲料であって、飲料中のポリフェノール濃度に対するコラーゲン濃度の比率が2.3以上である、発酵茶飲料またはコーヒー飲料(以下、それぞれを「本発明の発酵茶飲料」、「本発明のコーヒー飲料」といい、両者を併せて「本発明の飲料」ということがある)。
[2]ステイン付着抑制に用いるための、上記[1]に記載の飲料。
[3]ステイン付着が、発酵茶飲料またはコーヒー飲料の摂取並びに煙草の吸引および/またはステイン付着性食品の摂取に起因する、上記[1]または[2]に記載の飲料。
[4]飲料中のコラーゲン濃度が、0.1質量%以上である、上記[1]〜[3]のいずれかに記載の飲料。
[5]飲料中のポリフェノール濃度が、10〜60mg/100mLである、上記[1]〜[4]のいずれかに記載の飲料。
[6]飲料のpHが、5.5〜7.0の範囲内である、上記[1]〜[5]のいずれかに記載の飲料。
[7]紅茶飲料である、上記[1]〜[6]のいずれかに記載の飲料。
[8]コラーゲンを配合することを含んでなる、ステイン付着抑制に用いるための発酵茶飲料またはコーヒー飲料の製造方法であって、飲料中のポリフェノール濃度に対するコラーゲン濃度の比率が2.3以上となるようにコラーゲンを配合する、製造方法。
According to the present invention, the following inventions are provided.
[1] A fermented tea beverage or coffee beverage containing collagen, wherein the ratio of the collagen concentration to the polyphenol concentration in the beverage is 2.3 or more; It is referred to as the fermented tea beverage of the invention, "the coffee beverage of the invention", and both may be collectively referred to as "the beverage of the invention".
[2] The beverage according to the above [1] for use in stain adhesion suppression.
[3] The beverage according to the above [1] or [2], wherein stain adhesion is attributable to intake of fermented tea beverage or coffee beverage and intake of cigarette and / or intake of stain adherent food.
[4] The beverage according to any one of the above [1] to [3], wherein the collagen concentration in the beverage is 0.1% by mass or more.
[5] The beverage according to any one of the above [1] to [4], wherein the polyphenol concentration in the beverage is 10 to 60 mg / 100 mL.
[6] The beverage according to any one of the above [1] to [5], wherein the pH of the beverage is in the range of 5.5 to 7.0.
[7] The beverage according to any one of the above [1] to [6], which is a black tea beverage.
[8] A method for producing a fermented tea beverage or coffee beverage for use in stain adhesion inhibition, comprising blending collagen, wherein the ratio of collagen concentration to polyphenol concentration in the beverage is 2.3 or more So as to blend collagen, manufacturing method.
本発明の発酵茶飲料およびコーヒー飲料は、煙草の吸引や、ステイン付着性食品の摂取の際に一緒に摂取しても、飲料自身に含まれるポリフェノールに起因する歯牙へのステイン付着と、煙草の吸引および/またはステイン付着性食品の摂取に起因する歯牙へのステイン付着とを抑制することができるため、ステイン付着を懸念せずに摂取ができる点で有利である。 The fermented tea beverage and coffee beverage according to the present invention are stains on teeth caused by polyphenols contained in the beverage itself, even if they are inhaled together with cigarette suction and intake of stain-adherent food. Since stain adhesion to teeth due to intake of aspiration and / or stain adhesion food can be suppressed, it is advantageous in that intake can be performed without concern for stain adhesion.
本発明の飲料に配合されるコラーゲンは、家畜(例えば、ウシ、ブタ)や、家禽(例えば、ニワトリ)のコラーゲン組織(例えば、皮)から抽出したコラーゲンであってもよいし、魚類のコラーゲン組織から抽出したコラーゲンであってもよく、特に限定されるものではない。コラーゲンは、市販品を利用することもできる。 The collagen incorporated into the beverage of the present invention may be collagen extracted from collagen tissue (eg, skin) of domestic animals (eg, cattle, pigs) or poultry (eg, chicken) or fish collagen tissue It may be collagen extracted from the above, and is not particularly limited. A commercial item can also be utilized for collagen.
本発明においてコラーゲンは、飲料に溶解させて使用するため水溶性のものを使用することができる。ここで、水溶性コラーゲンとは、不溶性の天然コラーゲンが水溶性となるよう処理されたものを意味する。水溶性コラーゲンとしては、天然コラーゲンを熱変性させて得られたゼラチンや、天然コラーゲンまたはゼラチンを加水分解して得られたコラーゲンペプチドが挙げられ、これらをさらに加工した物(例えば、酵素修飾物、化学修飾物)であってもよい。 In the present invention, collagen can be used that is soluble in water for use after dissolving in a beverage. Here, water-soluble collagen means that insoluble natural collagen has been treated to be water-soluble. Examples of water-soluble collagen include gelatin obtained by heat-denaturing natural collagen, and collagen obtained by hydrolyzing natural collagen or gelatin, and further processing these (eg, enzyme-modified products, Chemically modified product).
コラーゲンの分子量は特に限定されないが、飲料に溶解させて使用することを考慮すると、例えば、重量平均分子量500〜300000(好ましくは1000〜200000、より好ましくは3000〜200000)のものを使用することができる。 The molecular weight of collagen is not particularly limited, but considering that it is dissolved in a beverage and used, for example, one having a weight average molecular weight of 500 to 300,000 (preferably 1,000 to 200,000, more preferably 30,000 to 200,000) may be used it can.
本発明においては、コラーゲンまたはゼラチンの加水分解物であるコラーゲンペプチドを有効成分として使用することができる。すなわち、本発明において「コラーゲン」はコラーゲンペプチドを含む意味で用いられる。コラーゲンペプチドは、コラーゲンまたはゼラチンを酵素や酸により加水分解して得られるペプチドであり、市販品を利用することもできる。 In the present invention, collagen peptides which are hydrolysates of collagen or gelatin can be used as an active ingredient. That is, in the present invention, "collagen" is used to include collagen peptide. A collagen peptide is a peptide obtained by hydrolyzing collagen or gelatin by an enzyme or an acid, and a commercially available product can also be used.
コラーゲンペプチドの分子量は特に限定されないが、飲料に溶解させて使用することを考慮すると、例えば、重量平均分子量500〜100000(好ましくは1000〜50000、より好ましくは3000〜20000)のものを使用することができる。ここで、本発明において使用されるコラーゲンおよびコラーゲンペプチドの重量平均分子量とは、分子量測定用プルランを標準として、ゲル浸透クロマトグラフィー(GPC)分析によって求めた値をいい、例えば、写真用ゼラチン試験法(PAGI法)第10版に記載されている方法で測定することができる。 The molecular weight of the collagen peptide is not particularly limited, but in consideration of dissolving it in a beverage, for example, one having a weight average molecular weight of 500 to 100,000 (preferably 1,000 to 50,000, more preferably 30,000 to 20,000) is used Can. Here, the weight-average molecular weight of collagen and collagen peptide used in the present invention refers to a value determined by gel permeation chromatography (GPC) analysis using pullulan for molecular weight measurement as a standard, for example, a gelatin test method for photographic use (PAGI method) It can be measured by the method described in the tenth edition.
本発明の飲料においては、飲料中のポリフェノール濃度に対するコラーゲン濃度の比率が所定値であることを特徴とする。本発明において、飲料中のポリフェノール濃度は、茶類のポリフェノール量を評価する際の基準である日本食品分析センター編「五訂 日本食品標準成分分析マニュアルの解説」(日本食品分析センター編、中央法規、2001年7月、p.252)に記載の公定法(酒石酸鉄吸光度法)を用いて測定することができる。また、飲料中のコラーゲン濃度は、コラーゲンの配合量に基づいて算出することができる。 The beverage of the present invention is characterized in that the ratio of the collagen concentration to the polyphenol concentration in the beverage is a predetermined value. In the present invention, the polyphenol concentration in the beverage is a standard for evaluating the polyphenol content of teas, edited by the Japan Food Analysis Center, edited by the Japanese Food Analysis Center, edited by the Japanese Food Analysis Center, edited by the Central Law. It can measure using the official method (iron tartrate absorptiometry) as described in July, 2001, p.252). Moreover, the collagen concentration in a drink can be calculated based on the compounding quantity of collagen.
ここで、酒石酸鉄吸光度法により測定したポリフェノール濃度の単位は「mg/100mL」であるが、コラーゲン濃度の単位が「質量%」の場合には、「質量%」に換算して上記比率を算出する。すなわち、Amg/100mLは0.001×A質量%に換算されるから、例えば、ポリフェノール濃度が45mg/100mLの場合には、0.045質量%に換算することができる。 Here, the unit of polyphenol concentration measured by the iron tartrate absorptiometry is “mg / 100 mL”, but when the unit of collagen concentration is “mass%”, the ratio is converted to “mass%” to calculate the above ratio Do. That is, since Amg / 100 mL is converted to 0.001 × A mass%, for example, when the polyphenol concentration is 45 mg / 100 mL, it can be converted to 0.045 mass%.
本発明の飲料においては、飲料中のポリフェノール濃度に対するコラーゲン濃度の比率の下限値が2.3以上(好ましくは3.5以上であり、より好ましくは4.6以上)である。飲料中のポリフェノール濃度に対するコラーゲン濃度の比率を上記のように特定することにより、煙草やステイン付着性食品に起因する歯牙へのステイン付着を効果的に抑制することができる。本発明の飲料においてはまた、飲料中のポリフェノール濃度に対するコラーゲン濃度の比率の上限値を200(好ましくは100)とすることができ、飲料中のポリフェノール濃度に対するコラーゲン濃度の比率の数値範囲は、例えば、2.3〜200(好ましくは4.6〜100)とすることができる。 In the beverage of the present invention, the lower limit value of the ratio of the collagen concentration to the polyphenol concentration in the beverage is 2.3 or more (preferably 3.5 or more, more preferably 4.6 or more). By specifying the ratio of the collagen concentration to the polyphenol concentration in the beverage as described above, it is possible to effectively suppress stain adhesion to teeth caused by tobacco or stain-adherent food. In the beverage of the present invention, the upper limit of the ratio of collagen concentration to polyphenol concentration in the beverage can be 200 (preferably 100), and the numerical range of the ratio of collagen concentration to polyphenol concentration in the beverage is, for example, 2.3-200 (preferably 4.6-100).
本発明の飲料におけるコラーゲン濃度は、飲料中のポリフェノール濃度に対するコラーゲン濃度の比率を満たしつつ、ステインの付着抑制効果をよりよく発揮させる観点から、例えば、下限値を0.1質量%(好ましくは0.2質量%)とすることができ、該濃度の上限値は2.0質量%(好ましくは1.0質量%)とすることができる。本発明の飲料中のコラーゲン濃度は、例えば、0.1〜2.0質量%または0.2〜1.0質量%とすることができる。 The collagen concentration in the beverage of the present invention has a lower limit of, for example, 0.1% by mass (preferably 0) from the viewpoint of exhibiting the adhesion suppression effect of stain better while satisfying the ratio of collagen concentration to polyphenol concentration in the beverage. And the upper limit value of the concentration may be 2.0 mass% (preferably 1.0 mass%). The collagen concentration in the beverage of the present invention can be, for example, 0.1 to 2.0% by mass or 0.2 to 1.0% by mass.
本発明の飲料中のポリフェノール濃度は、例えば、10〜60mg/100mLの範囲内とすることができ、好ましくは20〜50mg/100mL、より好ましくは20〜45mg/100mLの範囲内である。本発明の飲料中のポリフェノール濃度は、飲料の原料(例えば、茶葉抽出物および/または茶類エキス)のポリフェノール濃度を測定することにより、所定の範囲に調整することができる。あるいは、予めポリフェノール濃度が調整された茶葉抽出物および/または茶類エキスを飲料に対して所定量添加することによっても、飲料中のポリフェノール濃度の調整を行うこともできる。 The polyphenol concentration in the beverage of the present invention can be, for example, in the range of 10 to 60 mg / 100 mL, preferably in the range of 20 to 50 mg / 100 mL, more preferably in the range of 20 to 45 mg / 100 mL. The polyphenol concentration in the beverage of the present invention can be adjusted to a predetermined range by measuring the polyphenol concentration of the raw material of the beverage (for example, tea leaf extract and / or tea extract). Alternatively, the polyphenol concentration in the beverage can also be adjusted by adding a predetermined amount of tea leaf extract and / or teas extract in which the polyphenol concentration has been adjusted in advance to the beverage.
本発明において「発酵茶飲料」とは、紅茶飲料などの狭義の発酵茶飲料に加えて、烏龍茶飲料などの半発酵茶飲料を含む意味で用いられるものとする。 In the present invention, “fermented tea beverage” is used in a meaning including semi-fermented tea beverage such as oolong tea beverage in addition to narrowly-defined fermented tea beverage such as black tea beverage.
本発明の飲料は、ステインの付着抑制を目的として用いることができる。本発明において「ステイン付着」はヒトを含む哺乳動物の歯牙(特にヒトの歯)へのステイン付着を意味する。また、本発明において「ステイン付着」は、口腔を通じてなされる吸引または摂取に起因する着色現象を意味する。ステイン付着の原因としては、煙草の吸引、ステイン付着性食品の摂取が挙げられ、その原因物質としては、タール、ポリフェノール、アミノカルボニル反応物、アントシアニン、リコピン、カロテノイドが挙げられる。ポリフェノールを含有する食品としては、紅茶飲料などの茶飲料およびコーヒーや、ポリフェノールの一種であるクルクミンを含有するカレーが挙げられ、アミノカルボニル反応物を含有する食品としては、ソース、シロップが挙げられる。 The beverage of the present invention can be used for the purpose of inhibiting adhesion of stains. In the present invention, "stain adhesion" means stain adhesion to teeth of mammals including humans (especially human teeth). Also, in the present invention, "stain adhesion" means a coloring phenomenon caused by suction or intake made through the oral cavity. The causes of stain attachment include cigarette suction and intake of stain-adherent food, and the causes include tar, polyphenol, aminocarbonyl reactant, anthocyanin, lycopene and carotenoids. Foods containing polyphenols include tea beverages such as black tea beverages and coffee, and curries containing curcumin, which is a type of polyphenol, and foods containing aminocarbonyl reactants include sauces and syrups.
本発明において「ステイン付着抑制」とは、煙草中、あるいは、食品(例えば、茶飲料、コーヒー、ソース、カレー、果実、緑黄色野菜)中のステイン原因物質(例えば、ポリフェノール、タール、アミノカルボニル反応物、アントシアニン、リコピン、カロテノイド)の歯牙への付着が抑制されることをいう。 In the present invention, the "stain adhesion suppression" means a stain-causing substance (eg, polyphenol, tar, aminocarbonyl reactant) in tobacco or in food (eg, tea beverage, coffee, sauce, curry, fruit, greenish yellow vegetable) , Anthocyanins, lycopene, and carotenoids are inhibited from adhering to teeth.
本発明の飲料は、煙草の吸引前、吸引中および/または吸引後に口腔内に適用するか、あるいは摂取することで、煙草吸引に起因するステイン付着をよりよく抑制することができ、好ましくは煙草吸引直前、煙草吸引中および/または煙草吸引直後の口腔内への適用か、あるいは摂取である。本発明の飲料はまた、ステイン原因物質を含有する食品の摂取前、摂取中または摂取後に口腔内に適用するか、あるいは摂取することで、該食品の摂取に起因するステイン付着をよりよく抑制することができ、好ましくは該食品の摂取直前、摂取中および/または摂取直後の口腔内への適用か、あるいは摂取である。 The beverage of the present invention can be applied to the oral cavity before, during and / or after aspiration of a cigarette, or by taking it, the stain adhesion due to cigarette aspiration can be better suppressed, and preferably a cigarette. Application to the oral cavity immediately before, during and / or immediately after inhalation, or ingestion. The beverage of the present invention is also applied to the oral cavity before, during or after ingestion of the food containing the stain causative agent, or better taken to better inhibit stain adhesion due to ingestion of the food. Preferably, it is applied to the oral cavity immediately before, during and / or immediately after ingestion of the food, or ingestion.
後記例3に示されるように、発酵茶飲料の一種である紅茶飲料と一緒に煙草の吸引やステイン付着性食品の摂取をすると、歯牙へのステイン付着がより強まるが、本発明の発酵茶飲料やコーヒー飲料はこのような強いステイン付着を効果的に抑制できる点で有利である。すなわち、本発明によれば、ステイン付着を憂慮せずに、発酵茶飲料やコーヒー飲料を摂取しつつ、煙草の吸引やステイン付着性食品の摂取することができる。 As shown in Example 3 below, when suctioning tobacco or taking a stain-adherent food together with a black-tea beverage which is a type of fermented tea beverage, stain adhesion to teeth is enhanced, but the fermented tea beverage of the present invention And coffee beverages are advantageous in that such strong stain adhesion can be effectively suppressed. That is, according to the present invention, it is possible to suck tobacco or take stain-adherent food while taking in fermented tea beverages and coffee beverages without concern about stain adhesion.
本発明において、ステイン付着は、被験組成物浸漬前の歯牙と、着色原因物質存在下での被験組成物浸漬後の歯牙の歯牙表面の色差をL*a*b*表色系(JIS Z8722:2009)で測定することにより求めることができる(後記実施例の例1(3)参照)。被験組成物浸漬前の歯牙と、着色原因物質存在下での被験組成物浸漬後の歯牙の歯牙表面の色差が、対照組成物に浸漬した場合に比べて減少した被験組成物では、該被験組成物によりステイン付着が抑制されたと判断することができる。歯牙表面の色差は、例えば分光色差計により求めることができる。測定に用いる歯牙としては、例えば、ハイドロキシアパタイトペレット(ペンタックス社製)を用いることができる。 In the present invention, stain adhesion refers to the color difference between the tooth before immersion in the test composition and the tooth surface of the tooth after immersion in the test composition in the presence of a coloring agent in the L * a * b * color system (JIS Z8722: It can obtain | require by measuring by 2009) (refer Example 1 (3) of a postscript Example). In the test composition in which the color difference between the tooth before immersion of the test composition and the tooth surface of the tooth after immersion of the test composition in the presence of the coloring agent is reduced as compared to when immersed in the control composition, the test composition It can be determined that stain adhesion is suppressed by the substance. The color difference on the tooth surface can be determined, for example, by a spectrocolorimeter. As teeth used for measurement, for example, hydroxyapatite pellets (manufactured by PENTAX Corporation) can be used.
本発明においては、被験組成物浸漬前の歯牙と着色原因物質存在下での被験組成物浸漬後の歯牙の歯牙表面の色差Δbが、対照組成物浸漬後の歯牙の歯牙表面の色差Δbよりも小さかった場合に、ステイン付着が抑制されたと判定することができる。 In the present invention, the color difference Δb between the tooth before immersion of the test composition and the tooth surface of the tooth after immersion of the test composition in the presence of the coloring agent is higher than the color difference Δb of the tooth surface of tooth after immersion of the control composition. If smaller, it can be determined that stain adhesion is suppressed.
本発明の飲料では、通常の飲料の製造に用いられている飲料用添加剤、例えば、甘味料、酸味料、香料、色素、果汁、食品添加剤(例えば、起泡・泡持ち向上剤、苦味料、保存料、酸化防止剤、増粘安定剤、乳化剤、食物繊維、pH調整剤)などを添加してもよい。 In the beverage of the present invention, beverage additives used in the production of ordinary beverages, such as sweeteners, acidulants, flavors, dyes, fruit juices, food additives (eg, foam / foam retention improver, bitter taste) Additives, preservatives, antioxidants, thickening stabilizers, emulsifiers, dietary fibers, pH adjusters, etc. may be added.
本発明の飲料のpHは、5.5〜7.0(好ましくは5.5〜6.5)の範囲とすることができる。本発明の飲料のpHは、通常の飲料のpHを調整する手法により調整することができ、例えば、炭酸水素ナトリウムを用いて調整することができる。 The pH of the beverage of the present invention can be in the range of 5.5 to 7.0 (preferably 5.5 to 6.5). The pH of the beverage of the present invention can be adjusted by means of adjusting the pH of a normal beverage, for example, using sodium hydrogen carbonate.
本発明の飲料は、容器詰め飲料の形態で提供することができる。容器詰め飲料の容器とは、内容物と外気との接触を断つことができる密閉容器を意味し、例えば、金属缶、樽容器、プラスチック製ボトル(例えば、PETボトル、カップ)、紙容器、瓶、パウチ容器などが挙げられる。 The beverage of the present invention can be provided in the form of a containerized beverage. The container of the container-packed beverage means a closed container which can cut off the contact between the contents and the outside air, and, for example, a metal can, a barrel container, a plastic bottle (eg, PET bottle, cup), a paper container, a bottle And pouch containers.
本発明の飲料の製造は、コラーゲンを原材料に添加する点を除いては、発酵茶飲料やコーヒー飲料の製造に用いられる通常の方法に従って実施することができる。 The preparation of the beverage of the present invention can be carried out according to the usual method used for the preparation of fermented tea beverages and coffee beverages, except that collagen is added to the raw materials.
例えば、本発明の発酵茶飲料は、通常の発酵茶抽出液の調製に用いられている方法を用いて製造される茶抽出液から調製することができる。本発明において「茶抽出液」とは、茶葉を抽出処理に付することにより得られる、抽出液を意味する。以下、発酵茶飲料のうち紅茶飲料を例に調製方法を説明する。 For example, the fermented tea beverage of the present invention can be prepared from a tea extract produced using a method used for the preparation of a conventional fermented tea extract. In the present invention, "tea extract" means an extract obtained by subjecting tea leaves to an extraction treatment. Hereinafter, among the fermented tea beverages, a preparation method will be described by taking a black tea beverage as an example.
本発明に用いられる紅茶抽出液は、紅茶葉からの抽出液それ自体や、その加工品類(例えば、濃縮液体エキス、粉末エキス)などが挙げられ、従来、紅茶飲料の製造に用いられている紅茶抽出原料であれば、特に限定されず、適宜選択することができる。 The black tea extract used in the present invention includes the extract itself from black tea leaves and processed products thereof (for example, concentrated liquid extract, powder extract) and the like, and black tea conventionally used in the manufacture of black tea beverages It will not be specifically limited if it is an extraction raw material, It can select suitably.
紅茶葉の抽出処理は、特に限定されず、食品加工分野で一般的に用いられている種々の抽出方法を用いることができ、例えば、溶媒抽出、気流抽出、圧搾抽出などが包含され、必要に応じて、沈殿もしくは濾過などの固液分離、濃縮、遠心分離、乾燥(例えば、噴霧乾燥、凍結乾燥)または粉末化などの処理をさらに施してもよい。 The extraction process of black tea leaves is not particularly limited, and various extraction methods generally used in the field of food processing can be used, including, for example, solvent extraction, air flow extraction, squeezing extraction, etc. Depending on the situation, further processing such as solid-liquid separation such as precipitation or filtration, concentration, centrifugation, drying (for example, spray drying, lyophilization) or pulverization may be further performed.
ここで、溶媒抽出で用いられる抽出溶媒としては、水(例えば、硬水、軟水、イオン交換水および天然水)が望ましい。抽出溶媒の量は、当業者が適宜選択することができ、特に限定されない。例えば、抽出溶媒が水の場合は、その量は、茶葉の1〜100倍量(質量)である。抽出温度および時間は、当業者が適宜選択することができ、特に限定されない。例えば、抽出溶媒が水の場合は、その温度および時間は、10〜120℃で1分〜12時間である。 Here, water (for example, hard water, soft water, ion-exchanged water and natural water) is desirable as an extraction solvent used in the solvent extraction. The amount of extraction solvent can be appropriately selected by those skilled in the art and is not particularly limited. For example, when the extraction solvent is water, the amount is 1 to 100 times the amount (mass) of tea leaf. The extraction temperature and time can be appropriately selected by those skilled in the art and are not particularly limited. For example, when the extraction solvent is water, the temperature and time are 1 minute to 12 hours at 10 to 120 ° C.
抽出処理の一例としては、紅茶葉を、水中に、0〜90℃で、1分〜24時間浸漬および攪拌し、その後、紅茶葉を濾過または遠心分離する方法が挙げられる。ここで、抽出時の温度や時間などの条件は、特に限定されず、紅茶葉の種類や量によって当業者が任意に選択し、かつ設定することができる。 An example of the extraction treatment is a method of immersing and stirring black tea leaves in water at 0 to 90 ° C. for 1 minute to 24 hours, and then filtering or centrifuging the black tea leaves. Here, conditions such as temperature and time at the time of extraction are not particularly limited, and can be arbitrarily selected and set by those skilled in the art depending on the type and amount of black tea leaves.
本発明に用いられる紅茶葉は、茶期や、茶葉の形状、産地、品種や種類および等級などは限定されず、一般に飲用で市販されている乾燥茶葉を使用することができる。紅茶葉の種類は、例えば、スリランカ産茶葉(例えば、ウバ、ディンブラ、キャンディ、ラトナプラ、ギャル、ルフナ)、インド産茶葉(例えば、アッサム、ダージリン、ニルギリ)、ケニア、キーマン、ジャワとしてもよい。これらは1種類を使用してもよいし、2種以上を組み合わせて使用してもよい。 The tea leaves used in the present invention are not limited in the tea season, the shape of tea leaves, the place of production, the varieties, the types, the grade, etc. Dry tea leaves that are generally marketed for drinking can be used. The type of tea leaf may be, for example, tea leaf from Sri Lanka (eg, Uba, Dimbra, Kandy, Ratnapura, Gal, Ruhuna), tea leaf from India (eg, Assam, Darjeeling, Nilgiri), Kenya, Keyman, Java. One of these may be used, or two or more may be used in combination.
紅茶抽出液の調製において、紅茶エキスや紅茶パウダーなどの紅茶抽出液の濃縮物や精製物を用いてもよく、例えば、ポリフェノン(三井農林社製)などの市販品を用いることができる。また、これらの濃縮物や精製物は、そのまままたは水で溶解もしくは希釈したものを単独で使用しても、複数の種類を混合して用いても、紅茶抽出液と混合して用いてもよい。 In preparation of the black tea extract, a concentrate or purified product of the black tea extract such as black tea extract or black tea powder may be used, and for example, commercial products such as polyphenon (manufactured by Mitsui Norin Co., Ltd.) can be used. In addition, these concentrates and purified products may be used as they are or those dissolved or diluted with water alone, may be used as a mixture of a plurality of types, or may be mixed with a black tea extract. .
紅茶以外の発酵茶飲料(例えば、烏龍茶飲料)についても、紅茶飲料と同様に調製することができる。 Fermented tea beverages other than black tea (eg, oolong tea beverages) can also be prepared in the same manner as black tea beverages.
また、本発明のコーヒー飲料は、一般的な方法により抽出されたコーヒー抽出液から調製することができる。本発明において「コーヒー抽出液」とは、例えば、コーヒーミル(グラインダー)により粉砕した焙煎コーヒー豆から各種方法により得られる抽出液(いわゆるレギュラーコーヒー)のほか、コーヒーから抽出した成分を含有する液体がすべて包含され、コーヒー焙煎豆の冷水、温水、熱水、加圧熱水による抽出液や、プロピレングリコール水溶液、ショ糖脂肪酸エステルなどの食品添加物として許容されている界面活性剤の水溶液による抽出液、炭酸ガスなどの臨界抽出により得られた抽出液、インスタントコーヒーの溶解液なども包含される。コーヒー抽出液は上述したいずれであってもよいが、コーヒー抽出液は、好ましい態様によれば、焙煎したコーヒー豆を熱水(例えば、コーヒー豆の10倍量)で抽出した後、冷却してコーヒー抽出液とすることが好ましい。また、コーヒー豆からの抽出方法については、特に限定されず、例えば、ボイリング式、エスプレッソ式、サイフォン式、ドリップ式(例えば、ペーパー、ネル)が挙げられる。100gの抽出液を調製するために用いられるコーヒーの焙煎豆量は、例えば、5〜50gとすることができ、好ましくは10〜20gである。コーヒーの抽出は、例えば、焙煎コーヒー豆またはその粉砕物を水(20℃〜100℃)で5〜100分かけて抽出することにより実施できる。 Also, the coffee beverage of the present invention can be prepared from the coffee extract extracted by a general method. In the present invention, "coffee extract" refers to, for example, a liquid containing components extracted from coffee in addition to an extract (so-called regular coffee) obtained by roasted coffee beans ground by a coffee mill (grinder) by various methods. Are all included in the extract of coffee roasted beans with cold water, hot water, hot water, pressurized hot water, or aqueous solutions of surfactants accepted as food additives such as propylene glycol aqueous solution, sucrose fatty acid ester, etc. Also included are an extract, an extract obtained by critical extraction such as carbon dioxide gas, and a solution of instant coffee. The coffee extract may be any of those described above, but according to a preferred embodiment, the roasted coffee beans are extracted with hot water (for example, 10 times the amount of coffee beans) and then cooled. It is preferable to make a coffee extract. Moreover, it does not specifically limit about the extraction method from a coffee bean, For example, Boiling type, espresso type, siphon type, drip type (for example, paper, nel) are mentioned. The amount of roasted coffee beans used to prepare 100 g of extract can be, for example, 5 to 50 g, preferably 10 to 20 g. Extraction of coffee can be carried out, for example, by extracting roasted coffee beans or ground material thereof with water (20 ° C. to 100 ° C.) for 5 to 100 minutes.
本発明で用いられるコーヒー豆の種類は特に限定されないが、例えば、ブラジル、コロンビア、タンザニア、モカ、キリマンジェロ、マンデリン、ブルーマウンテンが挙げられ、これらの1種または2種以上をブレンドして用いても良い。コーヒー豆種としては、例えば、アラビカ種、ロブスタ種があり、好ましくは、香味の観点から、アラビカ種を用いることができる。コーヒー豆を焙煎により焙煎コーヒーとする方法については、特に限定されるものではなく、焙煎温度、焙煎環境についても限定されない。焙煎方法としては直火式、熱風式、半熱風式などが挙げられる。焙煎コーヒーの焙煎度としては特に限定されるものではなく、例えばライト、シナモン、ミディアム、ハイ、シティ、フルシティ、フレンチ、イタリアンのいずれを用いても良く、焙煎度の異なるコーヒー豆を複数種混合しても良い。 The type of coffee beans used in the present invention is not particularly limited, and examples thereof include Brazil, Colombia, Tanzania, Mocha, Kilimangero, Mandelin, Blue Mountain, etc. Even if one or more of these are blended and used good. Examples of the coffee bean species include arabica species and robusta species. Preferably, arabica species can be used from the viewpoint of flavor. It does not specifically limit about the method of making coffee beans into roasted coffee by roasting, It is not limited also about roasting temperature and roasting environment. As a roasting method, a direct fire type, a hot air type, a half hot air type, etc. may be mentioned. The degree of roasting of the roasted coffee is not particularly limited. For example, any of light, cinnamon, medium, high, city, full city, French and Italian may be used, and coffee beans having different degrees of roasting are used. Multiple types may be mixed.
本発明の飲料の製造に当たっては、当業界に公知の製造技術を用いて製造することができ、例えば、「改訂新版ソフトドリンクス」(株式会社光琳)を参考にすることができる。 In the production of the beverage of the present invention, it can be produced using a production technology known in the art. For example, "Revision new edition Soft Drinks" (Kosho Co., Ltd.) can be referred to.
本発明の飲料の製造手順において、コラーゲンの添加時期は特に制限されず、例えば、原材料の調合工程において添加することができる。発酵茶抽出物またはコーヒー抽出物にコラーゲンを添加する手順と、飲料用添加剤等の他の配合成分を添加する手順は、同時に実施しても、別々に実施してもよく、別々に実施する場合にはいずれが先であってもよい。 In the process for producing a beverage of the present invention, the addition time of collagen is not particularly limited, and can be added, for example, in the raw material preparation process. The procedure of adding collagen to the fermented tea extract or coffee extract and the procedure of adding other compounding ingredients such as beverage additives may be carried out simultaneously or separately or separately In any case, either one may come first.
本発明により提供される飲料は、抽出工程、調合工程、充填工程および殺菌工程などの工程を経て容器詰め飲料として提供することができる。例えば、調合工程で得られた飲料を常法に従って殺菌し、容器に充填することができる。殺菌は容器への充填前であっても充填後であってもよい。 The beverage provided by the present invention can be provided as a container-packed beverage through steps such as an extraction step, a preparation step, a filling step and a sterilization step. For example, the beverage obtained in the preparation process can be sterilized according to a conventional method and filled into a container. The sterilization may be before or after filling the container.
本発明の飲料の製造において殺菌処理を行う場合、食品分野で一般的に用いられている種々の殺菌方法を用いることができ、典型的には加熱殺菌法を用いることができる。使用できる殺菌方法としては、例えば、レトルト殺菌、UHT殺菌、低温殺菌、HTST殺菌などが挙げられる。これらの加熱殺菌方法は飲料の製造に通常用いられている条件で実施することができる。 When sterilization treatment is carried out in the production of the beverage of the present invention, various sterilization methods generally used in the food field can be used, and typically, heat sterilization can be used. Examples of sterilization methods that can be used include retort sterilization, UHT sterilization, pasteurization, HTST sterilization, and the like. These heat sterilization methods can be carried out under the conditions usually used for the production of beverages.
本発明の別の面によれば、コラーゲンを配合することを含んでなる、ステイン付着抑制に用いるための発酵茶飲料またはコーヒー飲料の製造方法であって、飲料中のポリフェノール濃度に対するコラーゲン濃度の比率が2.3以上となるようにコラーゲンを配合する、製造方法が提供される。本発明の製造方法は、本発明の飲料とその製造に関する記載に従って実施することができる。 According to another aspect of the present invention, there is provided a method of producing a fermented tea beverage or coffee beverage for use in stain adhesion inhibition comprising blending collagen, wherein the ratio of collagen concentration to polyphenol concentration in the beverage is used. There is provided a production method, wherein collagen is blended so as to be 2.3 or more. The production method of the present invention can be carried out according to the description of the beverage of the present invention and the production thereof.
以下の例に基づき本発明をより具体的に説明するが、本発明はこれらの例に限定されるものではない。 The present invention will be more specifically described based on the following examples, but the present invention is not limited to these examples.
例1:コラーゲン配合紅茶飲料によるステイン付着抑制効果(1)
(1)煙草抽出液の調製
吸引ろ過瓶に50gのmilliQ水を入れ、ガラス管を刺したゴム栓を取り付けた。ガラス管の先端に煙草(Marlboro gold 100’s(登録商標)、フィリップモリス社製)1本を刺して点火し、直ちにポンプで吸引した。先端から5cmの長さになるまで煙草を燃やし、吸引を終了した。合計10本の煙草について同様の操作を繰り返した。吸引ろ過瓶から液体を回収し、これを煙草抽出液とした。
Example 1: Stain adhesion inhibitory effect by collagen-containing black tea drink (1)
(1) Preparation of tobacco extract 50 g of milliQ water was put in a suction filtration bottle, and a rubber stopper pierced with a glass tube was attached. A tip of a glass tube was pierced with a cigarette (Marlboro gold 100's (registered trademark), manufactured by Philip Morris) to ignite it, and was immediately pumped. The cigarette was burned to a length of 5 cm from the tip and the aspiration ended. The same operation was repeated for a total of 10 cigarettes. The liquid was recovered from the suction filtration bottle and used as a tobacco extract.
(2)コラーゲン配合紅茶飲料の調製
紅茶葉50gを、70℃の熱水1500gで7分間撹拌しながら抽出した。得られた紅茶抽出液を用いてポリフェノール量が45mg/100mL(酒石酸鉄法にて測定)となるように配合量を決定し、ビタミンCを終濃度0.04%となるように抽出液に配合し、さらに、3種類の市販のコラーゲン(イクオスHDL−50DR(新田ゼラチン社製、重量平均分子量:5000)、水溶性コラーゲンペプチドSS(協和発酵バイオ社製、重量平均分子量:6600)、スーパーコラーゲンペプチドSCP−2000(新田ゼラチン社製、重量平均分子量:1100))をそれぞれ終濃度0.4%、0.2%、0.1%、0.05%となるように紅茶抽出液に配合した後、重曹を配合してpH6.2に合わせ、紅茶飲料調合液を得た。得られた調合液は缶に充填してレトルト殺菌(121℃、7分)処理した。対照飲料としては、市販コラーゲンを配合せず、同様の手順で調合および殺菌したサンプルを用いた。
(2) Preparation of Collagen-Containing Black Tea Beverage 50 g of black tea leaves were extracted with 1500 g of 70 ° C. hot water while stirring for 7 minutes. Using the obtained black tea extract, the amount is determined so that the polyphenol content is 45 mg / 100 mL (measured by the iron tartrate method), and vitamin C is added to the extract so that the final concentration is 0.04%. Furthermore, three types of commercially available collagen (Iquos HDL-50DR (manufactured by Nitta Gelatin Co., weight average molecular weight: 5000), water-soluble collagen peptide SS (manufactured by Kyowa Hakko Bio, weight average molecular weight: 6600), super collagen Peptide SCP-2000 (manufactured by Nitta Gelatin Co., weight average molecular weight: 1100) was added to the black tea extract so that the final concentration would be 0.4%, 0.2%, 0.1%, and 0.05%, respectively. After that, baking soda was added and adjusted to pH 6.2 to obtain a black tea beverage preparation. The resulting mixture was filled in cans and subjected to retort sterilization (121 ° C., 7 minutes). As a control drink, a commercial collagen was not mix | blended and the sample prepared and disinfected in the same procedure was used.
(3)着色試験
上記(1)で得られた煙草抽出液と上記(2)で得られた各紅茶飲料調合液を1:1(質量比)で混合して、各試験溶液を調製した。対照溶液は、上記(1)で得られた煙草抽出液と上記(2)で得られた対照飲料を1:1(質量比)で混合して調製した。各試験溶液によるステイン付着は、ハイドロキシアパタイトペレット(APP−100、PENTAX社、以下「HAP」という)を用いて評価した。分光色差計(SE7700、日本電色工業社製)を用いてHAPの表面を測色後、HAPを人工唾液(50mM塩化カリウム、1mM塩化カルシウム、0.1mM塩化マグネシウム、1mMリン酸2水素カリウム、1%アルブミン、pH7.0)に37℃で7時間浸漬した。浸漬後のHAPを全イオン交換水で洗浄した後、HAPを各試験溶液に37℃で24時間浸漬させた。浸漬後のHAPの表面を測色し、浸漬前後のHAP表面の色差Δbを算出した。色差Δbが対照溶液よりも低い値を示した試験溶液では、ステイン付着が抑制されたと判断した。着色試験は3回行い、色差Δbの平均値を求めた。
(3) Coloring Test The tobacco extract obtained in the above (1) and each black tea beverage preparation obtained in the above (2) were mixed at 1: 1 (mass ratio) to prepare each test solution. The control solution was prepared by mixing the cigarette extract obtained in the above (1) and the control beverage obtained in the above (2) in a 1: 1 ratio (by mass). The stain adhesion by each test solution was evaluated using a hydroxyapatite pellet (APP-100, PENTAX, hereinafter referred to as "HAP"). After measuring the surface of HAP using a spectrocolorimeter (SE 7700, manufactured by Nippon Denshoku Kogyo Co., Ltd.), HAP is artificial saliva (50 mM potassium chloride, 1 mM calcium chloride, 0.1 mM magnesium chloride, 1 mM potassium dihydrogen phosphate, It was immersed in 1% albumin, pH 7.0) at 37 ° C. for 7 hours. After washing the soaked HAP with total ion exchange water, the HAP was dipped in each test solution at 37 ° C. for 24 hours. The color of the surface of HAP after immersion was measured, and the color difference Δb of the surface of HAP before and after immersion was calculated. In the test solutions in which the color difference Δb showed a lower value than the control solution, it was judged that stain adhesion was suppressed. The coloring test was performed three times, and the average value of the color difference Δb was determined.
HAPの測色は、JIS Z8722:2009に基づくL*a*b*表色系のL*軸(明度)、a*軸(彩度:赤・緑軸)、b*軸(彩度:黄・青軸)で、反射測定条件、測定径φ6mmで上記分光色差計を用いて測定した。浸漬前のHAPの測色値をL1、a1、b1、浸漬後のHAPの測色値をL2、a2、b2とした時、以下の式により色差Δbとして算出した。
Δb=b2−b1
HAP colorimetry is based on JIS Z 8722: 2009. L * axis (brightness), a * axis (saturation: red / green axis), b * axis (saturation: yellow) of L * a * b * color system -Measurement was carried out using the above-mentioned spectrocolorimeter at a reflection measurement condition and a measurement diameter of φ 6 mm in the blue axis). When the colorimetric value of HAP before immersion is L1, a1, b1, and the colorimetric value of HAP after immersion is L2, a2, b2, the color difference Δb is calculated by the following equation.
Δb = b2-b1
(4)結果
結果は表1に示される通りであった。
コラーゲン添加量0.1%以上(ポリフェノール濃度に対するコラーゲン濃度が2.3倍以上)において、コラーゲン濃度依存的に煙草に起因するステイン付着が抑制された。 When the amount of collagen added was 0.1% or more (collagen concentration with respect to polyphenol concentration was 2.3 times or more), stain adhesion caused by cigarette was suppressed depending on collagen concentration.
例2:コラーゲン配合紅茶飲料によるステイン付着抑制効果(2)
(1)コラーゲン配合紅茶飲料の調製
コラーゲン配合紅茶飲料の調製は、例1(2)に記載の手順に従って行った。但し、市販のコラーゲンのうちイクオスHDL−50DRと、スーパーコラーゲンペプチドSCP−2000について試験を行い、終濃度0.2%、0.1%となるように紅茶抽出液に配合した。
Example 2: Stain adhesion inhibitory effect by collagen-containing black tea drink (2)
(1) Preparation of Collagen-Containing Black Tea Beverage A collagen-containing black tea beverage was prepared according to the procedure described in Example 1 (2). However, among the commercially available collagens, Iquos HDL-50DR and the super collagen peptide SCP-2000 were tested, and the final concentration was 0.2% and 0.1%, and the mixture was added to the black tea extract.
(2)着色試験
市販の中濃ソース(ブルドック中濃ソース、ブルドックソース社製)と上記(1)で得られた各紅茶飲料調合液を1:1(質量比)で混合して、各試験溶液を調製した。対照溶液は、市販の中濃ソースと上記(1)で得られた対照飲料を1:1(質量比)で混合して調製した。各試験溶液による着色試験は、例1(3)に記載の手順に従って行った。
(2) Coloring test A commercially available medium-density sauce (Brudoc medium concentration source, manufactured by Bourdocse) and each tea beverage preparation liquid obtained in the above (1) are mixed at 1: 1 (mass ratio), and each test is performed. The solution was prepared. The control solution was prepared by mixing a commercially available medium-concentrated source with the control beverage obtained in (1) above at a 1: 1 ratio (by mass). The color test with each test solution was conducted according to the procedure described in Example 1 (3).
(3)結果
結果は表2に示される通りであった。
コラーゲン添加量0.1%以上(ポリフェノール濃度に対するコラーゲン濃度が2.3倍以上)において、コラーゲン濃度依存的にソース摂取に起因するステイン付着が抑制された。 The stain adhesion caused by the intake of the source was suppressed depending on the collagen concentration at a collagen addition amount of 0.1% or more (collagen concentration relative to polyphenol concentration is 2.3 times or more).
例3:ステイン付着の程度
例1および2に示されるように、煙草やステイン付着性食品によるステイン付着がコラーゲンにより抑制されることが示されたが、本試験例では紅茶飲料がステイン付着にどの程度寄与しているかを確認した。
Example 3: Degree of stain adhesion As shown in Examples 1 and 2, it was shown that stain adhesion by cigarettes and stain adherent food was suppressed by collagen, but in this test example, the black tea beverage was found to be stain adherent. We confirmed whether it contributed to some extent.
(1)試験溶液の調製と着色試験
例1(1)で得られた煙草抽出液と、水あるいは例1(2)で得られた対照紅茶飲料とを1:1(質量比)で混合して、試験溶液1および2を調製した。また、市販の中濃ソース(ブルドック中濃ソース、ブルドックソース社製)と、水あるいは例1(2)で得られた対照紅茶飲料とを、1:1(質量比)で混合して、試験溶液3および4を調製した。対照溶液は、例1(2)で得られた対照紅茶飲料と水を1:1(質量比)で混合して調製した。対照溶液および各試験溶液による着色試験は、例1(3)に記載の手順に従って行った。
(1) Preparation of test solution and coloring test The tobacco extract obtained in Example 1 (1) and water or the control black tea drink obtained in Example 1 (2) were mixed at 1: 1 (mass ratio) Test solutions 1 and 2 were prepared. In addition, a test was conducted by mixing a commercially available medium-density sauce (Brudoc medium density sauce, manufactured by Bourdocse) with water or the control black tea beverage obtained in Example 1 (2) in a 1: 1 ratio (mass ratio). Solutions 3 and 4 were prepared. A control solution was prepared by mixing the control black tea beverage obtained in Example 1 (2) with water at 1: 1 (mass ratio). The color test with the control solution and each test solution was performed according to the procedure described in Example 1 (3).
(2)結果
結果は表3に示される通りであった。
対照溶液、試験溶液1および試験溶液2の比較から明らかなように、煙草と紅茶飲料とを組み合わせて適用した場合には、煙草・紅茶飲料単独よりも強いステイン付着が認められた。また、対照溶液、試験溶液3および試験溶液4の比較から明らかなように、ステイン付着性食品であるソースと紅茶飲料とを組み合わせて適用した場合にも、ソース・紅茶飲料単独よりも強いステイン付着が認められた。これらの結果から、本発明の飲料は、このような強いステイン付着の抑制に効果を発揮することが判明した。 As apparent from the comparison of the control solution, the test solution 1 and the test solution 2, when the tobacco and the black tea beverage were applied in combination, stain adhesion stronger than that of the tobacco and black tea beverage alone was observed. In addition, as apparent from the comparison of the control solution, the test solution 3 and the test solution 4, the stain adhesion stronger than that of the sauce / black tea beverage alone when applied in combination with the tea which is a stain adhering food Was recognized. From these results, it was found that the beverage of the present invention is effective in suppressing such strong stain adhesion.
Claims (8)
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