JP2018184346A - Inhalation fragrances, perfumes, and food fragrances - Google Patents

Abstract

An inhalation fragrance that can be taken regardless of oral administration is provided. An inhaled fragrance contains green leaf alcohol and linalool, and is liquid at room temperature with ethanol as a main solvent. The said green leaf alcohol is 0.02 mass% or more and 0.5 mass% or less with respect to the whole quantity, and the said linalool can be 0.005 mass% or more and 0.1 mass% or less with respect to the whole quantity. When inhaled fragrance is ingested nasally or pulmonarily, it exhibits any one or more of an antioxidant action against oxidative stress due to aging and an antioxidant action against oxidative stress caused by radiation irradiation. Inhaled fragrances exhibit antipsychotic stress effects when ingested nasally or pulmonary. [Selection] Figure 2

Description

  The present invention relates to an inhalation fragrance exhibiting an antioxidant action.

  Oxidation of the substances that make up the animal's body worsens physical condition, causes illness, and accelerates aging. Therefore, it is desirable to ingest a substance having an antioxidant effect that suppresses oxidation. Conventionally, it has been known that familiar products such as green tea have an antioxidant effect and an anti-aging effect (for example, Patent Document 1).

  However, it is difficult to take an amount that can be said to be effective in practice. Although the antioxidant mechanism when ingested is not well understood, the amount of active ingredient absorbed from the intestinal tract is very small compared to the intake and must be drastically consumed to achieve the desired effect. I must.

  On the other hand, when green tea extract is used as a fragrance, it is known to have a sedative effect (for example, Patent Document 2).

  However, an antioxidant inhalation fragrance containing green tea ingredients is not known.

JP 2004-2237 A Japanese Patent No. 5306160

  The problem to be solved by the present invention is to provide an inhalation fragrance that can be taken regardless of oral administration.

  In order to solve the above-mentioned problems, the inhalation fragrance according to the present invention contains green leaf alcohol and linalool, and is liquid at room temperature using ethanol as a main solvent.

  ADVANTAGE OF THE INVENTION According to this invention, the inhalation fragrance | flavor which can be ingested irrespective of oral administration can be provided.

External view of metabolic gauge. The figure which shows the quantity of 8-OHdG in the urine accompanying the aging in Experiment 1. FIG. The figure which shows the quantity of 8-OHdG in the urine accompanying the radiation irradiation in Experiment 2. FIG. The figure which shows the time-dependent change of the amount of drinking in Experiment 3. FIG.

  Hereinafter, inhalation fragrance | flavor, perfume, and foodstuff concerning one Embodiment of this invention are demonstrated, referring drawings.

  The inhalation fragrance | flavor of this embodiment contains green leaf alcohol and linalool as an active ingredient. Further, the inhaled fragrance may further contain other active ingredients such as benzyl alcohol and impurities. The inhaled fragrance is liquid at room temperature (18 ° C. to 25 ° C.). The main solvent of the inhaled fragrance can be ethanol. Therefore, when the inhaled fragrance is heated to about 35 ° C. to 37 ° C., which is about the temperature of human body temperature, the green leaves alcohol and linalool which are active ingredients are volatilized, and humans and animals can inhale. Moreover, the inhaled fragrance gradually evaporates even at room temperature, and can be inhaled by humans and animals.

  Green leaf alcohol is another name for cis-3-hexen-1-ol, which is called hexenol, and is a substance contained in tea leaves. Green leaf alcohol may contain an isomer. Ethanol is a good solvent for green leaf alcohol.

  Linalool refers to 3,7-dimethyl-1,6-octadien-3-ol and is a substance contained in tea leaves. Ethanol is a good solvent for linalool.

  As a more desirable composition of the inhalation fragrance, green leaf alcohol is contained in an amount of 0.02 to 0.5% by mass and linalool is contained in an amount of 0.005 to 0.1% by mass with respect to the total amount. The balance can be ethanol and water. In this case, for example, ethanol can be 99.9% by mass and water can be the balance.

  Even if the inhalation fragrance contains impurities, other fragrances, surfactants, coloring agents, etc., the effect of the inhalation fragrance is not lost. Here, the contaminants mean those that have little influence on the effect of the inhaled fragrance even if included in the inhaled fragrance. Some contaminants may be desirable in inhalation fragrances.

  The inhaled fragrance having the above composition has a fragrance, and has an antioxidant effect when inhaled by humans and animals, that is, when ingested nasally or pulmonary. The antioxidant action includes an antioxidant action against oxidation due to aging and an antioxidant action against radiation stress.

  Aoba alcohol, which is one of the active ingredients of inhaled fragrances, has conventionally been discarded as steam in the stage of tea making. Therefore, green tea alcohol is hardly contained in tea products such as Sencha.

  On the other hand, the inhalation fragrance | flavor of this embodiment can be manufactured by collect | recovering the steams obtained when steaming raw tea leaves, distilling and concentrating this. Therefore, the inhaled fragrance is richer in green leaf alcohol and linalool than tea products. If the inhalation fragrance | flavor of this embodiment is ingested nasally thru | or pulmonary, it can ingest green leaf alcohol and linalool more efficiently with respect to the ingestion of the green tea which put tea products, such as Sencha, with hot water. For this reason, according to the inhalation fragrance | flavor, the antioxidant effect with respect to the oxidation by aging which cannot be obtained only by ingesting green tea, and the antioxidant effect with respect to irradiation stress can be obtained.

  The mechanisms by which green leaf alcohol and linalool exhibit an antioxidant action against oxidation due to aging and an antioxidant action against radiation stress in animals such as humans and mice are not well understood. In the experiment, an antipsychotic stress effect such as a high relaxation effect has been observed, for example, the amount of water consumed by mice is significantly less in the administration group than in the control.

  The inhaled fragrance can be used as it is or as a perfume containing other fragrances, surfactants and coloring agents. Even if this perfume contains other fragrances, various solvents, surfactants, coloring agents, etc., it does not lose the above-mentioned antioxidant action and antipsychotic stress action.

  The inhalation fragrance may contain other active ingredients. Other active ingredients include benzyl alcohol, methyl salicylate, geraniol, 2-phenylethanol, β-ionone, (E) -nerolidol, indole and the like. These other active ingredients enhance the antioxidant and antipsychotic stress effects of green leaf alcohol and linalool. For example, if benzyl alcohol is contained in an inhaled fragrance, the depth of the scent will come out. When the inhaled fragrance contains benzyl alcohol, the preferable mixing ratio is 0.001% by mass or more and 0.05% by mass or less based on the total amount of the inhaled fragrance.

  The inhaled fragrance can be used as it is or as it is or as a food fragrance containing a surfactant and a coloring agent. For example, sprinkling inhalation fragrance on powdered tea and pouring hot water can make a fragrant tea. When drinking this tea, the components of the inhaled fragrance are absorbed nasally or pulmonarily and exhibit antioxidant and antipsychotic stress effects.

  Here, the definition of “oxidative stress” by the Japanese Antioxidant Society is “the difference between the oxidative damage potential of the active oxygen group generated in vivo and the antioxidant potential of the in vivo antioxidant system”. In other words, the state where “oxidation stress” is present is a state where an excessive active oxygen group that cannot be captured by the in-vivo antioxidant system is generated. The inhalation fragrance | flavor of this embodiment is shown by the experiment 1 and experiment 2 which are shown below that there exists an effect | action which improves thru | or prevents the state with this "oxidative stress".

  Even if the cause of “oxidation stress” is aging or radiation, there is no great difference in the qualitative state that this “oxidation stress” exists. If it does so, it can be said that the inhalation fragrance | flavor of this embodiment has an antioxidant effect not only to oxidative stress by aging or radiation, but also to oxidative stress by other causes, such as air pollution and tobacco.

  Hereinafter, more specific description will be made using examples.

(Manufacture of inhalation fragrance)
The inhalation fragrance is described in detail in Japanese Patent Application Laid-Open No. 2015-109832 relating to the applicant's patent application. In brief, fresh tea leaves are steamed with steam at about 100 ° C. for 30 to 120 seconds. This steam is recovered, cooled to about 40 ° C. to 60 ° C. and liquefied to obtain a stock solution. This stock solution is extracted with hexane, and a liquid inhalation fragrance is obtained at room temperature (18 ° C. to 25 ° C.) with the above composition using ethanol as a solvent.

(Prescription example)
(Prescription Example 1)
Aoba alcohol: 0.02 mass%
Linalool: 0.005 mass%
Ethanol: 99.9% by mass
Water: the rest

(Prescription example 2)
Aoba alcohol: 0.2% by mass
Linalool: 0.03 mass%
Ethanol: 99.9% by mass
Water: the rest

(Prescription Example 3)
Aoba alcohol: 0.5% by mass
Linalool: 0.1% by mass
Benzyl alcohol: 0.001% by mass
Ethanol: 99.2% by mass
Other active ingredients: 0.1% by mass
Other active ingredients include methyl salicylate, geraniol, 2-phenylethanol, β-ionone, (E) -nerolidol, and indole in appropriate amounts.
Contaminants: 0.05% by mass
The main impurities include 1-penten-3-ol, heptanal, (Z) -3-hexanol, octanol.
Water: the rest

(Prescription Example 4)
Aoba alcohol: 0.5% by mass
Linalool: 0.05% by mass
Benzyl alcohol: 0.05% by mass
Ethanol: 99.2% by mass
Other active ingredients: 0.1% by mass
Other active ingredients include methyl salicylate, geraniol, 2-phenylethanol, β-ionone, (E) -nerolidol, and indole in appropriate amounts.
Contaminants: 0.05% by mass
The main impurities include 1-penten-3-ol, heptanal, (Z) -3-hexanol, octanol.
Water: balance

(Formulation example 5) Perfume example Aoba alcohol: 0.5 mass%
Linalool: 0.05% by mass
Benzyl alcohol: 0.05% by mass
Ethanol: 97.1% by mass
Other active ingredients: 0.1% by mass
Other active ingredients include methyl salicylate, geraniol, 2-phenylethanol, β-ionone, (E) -nerolidol, and indole in appropriate amounts.
Contaminants: 0.05% by mass
The main impurities include 1-penten-3-ol, heptanal, (Z) -3-hexanol, octanol.
Limonene: 0.2% by weight (as other fragrance)
Surfactant: 1.8% by weight
Coloring agent: Appropriate amount Water: Balance

(Formulation Example 6) Examples of food flavors Aoba alcohol: 0.5% by mass
Linalool: 0.05% by mass
Benzyl alcohol: 0.05% by mass
Ethanol: 98% by mass
Other active ingredients: 0.1% by mass
Other active ingredients include methyl salicylate, geraniol, 2-phenylethanol, β-ionone, (E) -nerolidol, and indole in appropriate amounts.
Contaminants: 0.05% by mass
The main impurities include 1-penten-3-ol, heptanal, (Z) -3-hexanol, octanol.
Surfactant: 1% by mass
Coloring agent: Appropriate amount Water: Balance

(Experiment on antioxidant effect)
Ten females of ICR mice were put into the metabolic gauge 1 one by one, 5 were control (control group), and 5 were treated groups. FIG. 1 is an external view of the metabolic gauge 1. As shown in FIG. 1, in the metabolic gauge 1, a water feeder 13 and a feeder 14 are connected to a main room 12 in which a mouse is placed. A net 15 is stretched in the main room 12 and a mouse is placed on the net 15. Below the net 15 is connected an excrement container 16 for separating and accumulating feces and urine. All experiments were performed at room temperature.

  A lid 11 is installed in the main room. The lid portion 11 has a plurality of intake holes. A non-woven fabric is disposed at a portion corresponding to the intake hole. An inhalation fragrance was dropped onto the nonwoven fabric to expose the mouse, and the mouse was exposed to spontaneous breathing, whereby the inhalation fragrance was ingested nasally or through the lungs.

  In each of the following experiments, the above-described Formulation Example 1 to Formulation Example 5 were used, but since the experimental results are almost the same in each Formulation Example, only the results of Formulation Example 3 are shown.

(Experiment 1) Verification of antioxidant action against oxidation due to aging 20 μl of daily inhaled fragrance until 2 weeks after birth of newborn mice, 200 μl of daily inhaled fragrance from 2 weeks onwards are dropped on the nonwoven fabric installed on the lid 11 Urine 8-OHdG was measured. The control received no inhalation fragrance.

  8-OHdG (8-hydroxy-2'-deoxyguanosine) is a DNA oxidative damage marker and is often measured when measuring oxidative stress due to active oxygen. The greater the oxidative stress, the more 8-OHdG in the urine. In this experiment, 8-OHdG in urine was measured as follows.

  Mix 50 μl of urine and 50 μl of diluent containing ribonucleoside 8-OHGuo. After mixing, the supernatant from which the precipitate was removed by centrifugation was separated by column switching high performance liquid chromatography combining two types of columns of anion exchange and reverse phase, and 8-OHdG was detected by an electrochemical detector (Coulochem manufactured by ESA). Measure according to III).

  FIG. 2 is a diagram showing the amount of 8-OHdG in urine accompanying aging in Experiment 2 (the amount of 8-OHdG relative to 1 mg of creatinine (ng)). The horizontal axis represents time, and the vertical axis represents the amount of 8-OHdG.

  As shown in FIG. 2, the amount of 8-OHdG in urine increased with aging in both the control indicated by ◯ and the administration group indicated by ▪. However, the rate of increase in the administration group was clearly lower than the control, with a significant difference of less than 5% risk.

(Experiment 2) Verification of antioxidant action against radiation stress Five ICR mice were divided into the following groups, and comparative experiments were performed.
Group 1: Control (does not administer inhalation fragrance and does not receive radiation)
Second group: The control is irradiated with 0.5 Gy of radiation only once.
・ Group 3: Inhalation fragrance is administered and no radiation is applied.
Group 4: Inhalation fragrance is administered and 0.5 Gy of radiation is irradiated only once.
In the administration group, 200 μl of inhalation fragrance was dropped on a non-woven fabric placed on the lid 11 every day and administered nasally or pulmonary by natural inhalation. The control received no inhalation fragrance.

  For radiation, whole body irradiation of 0.5 Gy was performed using an X-ray irradiation apparatus (manufactured by Hitachi Engineering & Service, MBR-1520) at an irradiation dose rate of 0.68 Gy / min.

  FIG. 3 is a diagram showing the amount of 8-OHdG in urine accompanying irradiation with radiation in Experiment 2 (the amount of 8-OHdG (ng) relative to 1 mg of creatinine). The horizontal axis represents time, and the vertical axis represents the amount of 8-OHdG.

  For each group, when irradiated before administration (0 week in FIG. 3), when irradiated after 1 week (1 week in FIG. 3), when irradiated after 7 weeks (7 week in FIG. 3) The amount of 8-OHdG in urine (the amount of 8-OHdG per 1 mg of creatinine (ng)) was measured. The method for measuring the amount of 8-OHdG in urine is the same as that in Experiment 1.

  As shown in FIG. 3, the amount of 8-OHdG in urine increased with time in both the second group and the fourth group irradiated with radiation. The amount of 8-OHdG in the urine of the second group clearly increased with a significant difference of less than 5% of the risk rate with respect to the first group after the first week and after the seventh week. However, the amount of 8-OHdG in the urine of the fourth group was not significantly different from that of the third group when administered for 1 week and when administered for 7 weeks.

(Experiment 3) Verification of antipsychotic stress action Five ICR mice were divided into control and administration groups. In the administration group, 200 μl of inhalation fragrance was dropped on a non-woven fabric placed on the lid 11 every day and administered nasally or pulmonary by natural inhalation. The control received no inhalation fragrance. And the time-dependent change of the amount of drinking water was compared about each group.

  FIG. 4 is a diagram showing the change over time in the amount of water consumed in Experiment 3. As shown in FIG. 4, in the first to third weeks after the start of administration, the amount of water consumed in the administration group was significantly less than the control due to a risk rate of less than 5%.

  Here, there is a report that the amount of water consumed by mice increases due to stress (for example, T. Goto et la., Behavioral Brain Research 270 (2014) 339-348). Therefore, it was suggested that nasal or pulmonary administration of inhaled fragrance has antipsychotic stress action or relaxation effect.

(Summary)
From the above experimental results, it was found that the inhaled fragrance has an antioxidant action against oxidation due to aging and an antioxidant action against radiation stress when inhaled, that is, taken nasally or pulmonary. . Further, it was found that the inhaled fragrance is effective even if it contains impurities and other fragrances. Furthermore, it was found that when inhaled fragrance is inhaled, it has antipsychotic stress action.

  In the above experiment, an animal (mouse) was used, but it is natural that the same effect can be obtained for humans.

  As described above, the inhalation fragrance of this embodiment contains green leaf alcohol and linalool as active ingredients, and ethanol is the main solvent. Therefore, it is easy to ingest the inhaled fragrance nasally or pulmonarily, and by nasal or pulmonary intake, it exhibits an antioxidant action against oxidation due to aging and an antioxidant action against radiation stress. In addition, a high antipsychotic stress action can be obtained by ingesting the inhaled fragrance nasally or through the lungs.

1 Metabolism gauge 11 Lid 12 Main room

Claims (6)

Aoba alcohol,
Linalool,
An inhalation fragrance that is liquid at room temperature using ethanol as the main solvent. The green leaf alcohol is
0.02% by mass or more and 0.5% by mass or less with respect to the total amount,
The linalool is
The inhalation fragrance according to claim 1, wherein the amount is 0.005% by mass or more and 0.1% by mass or less based on the total amount.   The inhalation fragrance | flavor of Claim 1 or Claim 2 which exhibits the antioxidant effect with respect to oxidative stress.   The inhalation fragrance | flavor of any one of Claim 1- Claim 3 which exhibits an antipsychotic stress effect | action.   A perfume which is liquid at room temperature and contains the inhaled fragrance according to any one of claims 1 to 4. A food fragrance that contains the inhaled fragrance according to any one of claims 1 to 4 and is liquid at room temperature.