JP2018035118A - Collagen production promoting action of defatted perilla frutescens extract - Google Patents

Abstract

PROBLEM TO BE SOLVED: To solve the problem that the efficacy of defatted Perilla frutescens after oil squeezing has been found out but not enough and a lot of defatted Perilla frutescens is discarded; though, on the other hand, the "Perilla frutescens oil" squeezed oil from Perilla frutescens seeds is distributed widely as a food and has attracted attention as a health food and, is also utilized for a cosmetic not only as a food but also as an oil and fat for compensating sebum, the production of Perilla frutescens seeds has been increasing every year, and Perilla frutescens cultivation spreads in various places.SOLUTION: Newly, we found out a collagen production promoting action for a defatted Perilla frutescens extract and newly provide a collagen production promoter of defatted Perilla frutescens extract, a periodontitis improving agent of defatted Perilla frutescens extract based on a collagen production promoting effect, and an application to moisturizing cosmetics.SELECTED DRAWING: None

Description

  The present invention relates to a novel use of defatted egoma based on the collagen production promoting action of the defatted egoma extract.

  In recent years, since sesame contains a large amount of α-linolenic acid, which is an essential fatty acid, “egoma oil” extracted from sesame seeds has been widely distributed as food and has attracted attention as a health food. In addition to being edible, it is also used in cosmetics as a fat to supplement sebum, so the production of sesame seeds is increasing year by year, and sesame cultivation is spreading in various places.

  On the other hand, sesame seeds after oil extraction (hereinafter referred to as defatted sesame seeds) also contain a large amount of polyphenols such as rosmarinic acid and luteolin, and are therefore used as UV absorbers extracted from defatted sesame seeds with organic solvents such as ethanol and acetone. (See Patent Document 1), oral antibacterial action (see Patent Document 2), and histamine release inhibitory action (see Patent Document 3), are expected to be effectively used as food and cosmetics. However, the effectiveness is not sufficient, and many degreasing egomas are discarded.

  In addition, with regard to the collagen production promoting action found in the present invention, collagen is present in tissues of mammals such as humans such as skin, bone, cartilage and blood vessels (collagen type I is mainly used for skin, bone and tendon, collagen type II. Collagen type III is present in blood vessels, collagen type IV is present in the basement membrane), and forms a fibrous structure to physically support each tissue. It is considered a thing.

  In the field of oral care, destruction of periodontal tissue is considered as a factor of periodontal disease, and it is considered that promoting collagen production is effective in the treatment and prevention of periodontal disease (patent document) 4). In the field of skin care, collagen supports the skin structure with the dermis layer and is known to retain moisture. (See Patent Document 5).

(Patent Document 1) Japanese Patent Application Laid-Open No. 2002-212026
(Patent Document 2) JP 2000-239136 A
(Patent Document 3) JP 2000-086510 A
(Patent Literature 4) JP 2006-31613 A
(Patent Document 5) JP-A 2006-176425

  Therefore, the present invention provides a new use of the defatted sesame seed by newly finding the physiological function of the defatted sesame extract, and aims at effective utilization of resources.

  The present inventors have found a collagen production promoting action for a defatted sesame extract, a collagen production promoter of the defatted sesame extract, and a periodontal disease improving agent of the defatted sesame extract based on the collagen production promoting effect, to moisturizing cosmetics. Providing new uses.

  ADVANTAGE OF THE INVENTION According to this invention, the novel collagen production promoter which improves a periodontal disease effectively and improves the moisture retention function of skin can be provided.

Cytotoxicity test results of defatted egoma extract Results of promotion of collagen production by defatted egoma extract

  The term “egoma” in the present invention refers to egoma (Linaceae, Perilla frutescens var. Frutescens) belonging to the genus Lamiaceae (Perilla).

  Collagen production promoters in the present invention are ampoules, capsules, powders, granules, pills, tablets, solids, liquids, gels, bubbles, emulsions, creams, ointments, sheets, Mousse, powder-dispersed, multi-layered, aerosol-like pharmaceuticals, quasi-drugs, cosmetics, toothpaste such as toothpaste, toothpaste, liquid toothpaste, mouthwash, gel, ointment, in mouth It can be used in various dosage forms such as a refreshing agent, a tablet for gargle, and a gum.

  The periodontal disease-improving agent in the present invention can take a liquid form (liquid, liquid, paste form), solid form (solid, solid form), etc. Examples of dosage forms include toothpaste, liquid toothpaste. , Toothpaste compositions such as liquid dentifrice, powder dentifrice, mouthwash composition, coating composition, oral pasta, mouth freshener composition, food form (eg chewing gum, tablet candy, candy, gummi, film, Troches, etc.).

  The periodontal disease-improving agent includes components usually used in oral compositions, such as sucrose fatty acid ester, maltose fatty acid ester, alkyldiaminoethylglycine hydrochloride, laurylmethylamino, as long as the effects of the present invention are not impaired. Surfactants such as betaine acetate and sodium N-acyl sarcosine, perfumes such as mastic oil, parsley oil, anise oil, strawberry flavor and apple flavor, acidulants such as citric acid and malic acid, lubricants such as glycerin fatty acid ester , Polishing agents such as silica gel, aluminosilicate, coloring agents such as safflower red pigment and gardenia yellow, pH adjusting agents such as citric acid and malic acid, preservatives such as methyl paraben and ethyl paraben, brighteners such as shellac and carnauba wax , Fluidizing agents such as fine particle silicon dioxide, neutralizing agents, pullulan, Binders such as latin, thickeners such as propylene glycol and butylene glycol, preservatives such as methyl paraben and ethyl paraben, disintegrating agents such as pregelatinized starch and sodium alginate, solvents such as water, ethanol and propanol, starch syrup, glucose, An excipient such as fructose and a sweetener such as stevia and sucralose can be appropriately blended depending on the purpose.

  The dosage form of the moisturizing cosmetic composition in the present invention is not particularly limited as long as the desired effect is sufficiently exhibited. For example, it can be used as various dosage forms such as liquid, emulsion, cream, and gel. it can. Further, the dosage form is liquid or emulsion, and it can be used by impregnating a carrier such as a nonwoven fabric or cotton.

  In the moisturizing cosmetic, within the range that does not impair the effects of the present invention, components usually used in cosmetics, for example, lower alcohols such as ethanol and isopropanol; glyceryl monostearate, polyoxyethylene sorbitan monostearate , Polyoxyethylene hydrogenated castor oil, polyoxyethylene polyoxypropylene decyl tetradecyl ether, decaglyceryl monolaurate, diglyceryl monostearate, ethylene glycol monostearate, sorbitan monoisostearate, coconut oil fatty acid monoethanolamide, saponin Nonionic surfactants such as: N-stearoyl-L-glutamate sodium, cocoyl methyl taurate sodium, potassium stearate, polyoxyethylene sulfosuccinate lauryl dinato Anionic surfactants such as um; cationic surfactants such as stearyltrimethylammonium chloride, N-coconut oil fatty acid acyl-L-arginine ethyl DL-pyrrolidone carboxylate, dimethylaminopropylamide stearate; lauryl Such as dimethylaminoacetic acid betaine, 2-alkyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, N- [3-alkyl (12,14) oxy-2-hydroxypropyl] -L-arginine hydrochloride solution, etc. Amphoteric surfactant: xanthan gum, carboxyvinyl polymer, acrylic acid / alkyl methacrylate copolymer, (hydroxyethyl acrylate / acryloyldimethyltaurine sodium) copolymer, (acryloyldimethyltaurine ammonium methacrylate) Lioxyethylene (25) beenyl ether) cross-polymer, (acrylic acid / vinyl pyrrolidone) copolymer, (acrylates / polyoxyethylene (25) behenyl ether methacrylate) copolymer, (dimethylacrylamide / ethyltrimonium chloride methacrylate) copolymer , Hydroxypropylcellulose, hydroxypropylated phosphate cross-linked starch, hectorite, bentonite, silicic anhydride and other water-soluble thickeners; vitamins such as tocopherol and its derivatives, ascorbic acid and its derivatives; dibutylhydroxytoluene, sulfite Antioxidants such as phenoxyethanol, octoxyglycerin and parabens; UV absorbers such as 2-ethylhexyl paramethoxycinnamate; inorganic pigments, Lubricating agents, plant extract extracts, sequestering agents, fragrances, pH adjusters and the like can be appropriately blended depending on the purpose.

  Hereinafter, the present invention will be described in more detail with reference to examples such as production examples of defatted egoma extract, but the present invention is not limited thereto.

(Production Example 1)
Use commercially available degreasing egoma. After grinding about 20 g of defatted sesame seeds, 80 mL of hexane was added, left standing at room temperature for 24 hours, and the residue was collected by suction filtration. After washing with diethyl ether, a dry powder was obtained. Then, 50 mL of butanol was added to the dry powder, and after stirring for 1 hour, the residue was collected by suction filtration. After washing thoroughly with methanol, the residue was collected by suction filtration, and was added to diethyl ether. After washing, a dry powder was obtained. Furthermore, 200 mL of water was added to the powder, and the supernatant was collected by centrifugation. The same operation was performed, and the supernatants were combined and freeze-dried twice to obtain about 2 g of dry powder. Water was added thereto to adjust the concentration to 0.25 mg / mL, and a defatted egoma extract was obtained.

(Test Example 1) Cytotoxicity test
Inoculate mouse fibroblasts (LM cells) into a 96-well plate in 0.5% BPM199 medium (mixed with Medium 199 (Gibco) liquid medium and 0.5% Bacto Pepton (Japan BD)) to 4.5 × 10 ³ cells / well. And cultured for 48 hours at 37 ° C. and 5% CO ₂ . After the cultivation, the whole medium was removed, and 100 μL of the defatted sesame extract obtained in Production Example 1 was added to the medium, followed by culturing at 37 ° C. under 5% CO ₂ for 24 hours. After culture, remove the entire volume of the medium, add 5% Cell Counting Kit Wst-8 (Dojindo Laboratories) to the medium, and incubate at 37 ° C under 5% CO ₂ for 1 hour. Absorbance (450 nm) Was measured.

(Results of cytotoxicity test)
As shown in FIG. 1, the defatted egoma extract did not show cytotoxicity.

(Test Example 2) Collagen production promotion test
LM cells were inoculated in a 0.5-well BPM199 medium at a concentration of 1.0 × 10 ⁵ cells / well in a 6-well plate and cultured at 37 ° C. under 5% CO ₂ for 48 hours. After culturing, the whole medium was removed, and the defatted sesame extract obtained in Production Example 1 was added to the medium to a concentration of 0.25 mg / mL, followed by culturing at 37 ° C. under 5% CO ₂ for 48 hours. After culturing, the supernatant was collected and concentrated to 1 mL after adding Protease Inhibitor Cocktail for General Use (Nacalai Tesque). Dissolve Sirius Red F3B (Waldeck) in 0.5 M acetic acid to 1 mg / mL, mix with the supernatant in an equivalent amount, remove the supernatant by centrifugation, and add 0.5 mL of 0.1 M NaOH to the precipitate and stir. , Dissolved. The absorbance (540 nm) of this solution was measured, and the amount of type I collagen was calculated from a calibration curve of a soluble type I collagen standard solution.

(Results of collagen production promotion test)
As shown in FIG. 2, the defatted egoma extract showed an effect of promoting collagen production, suggesting that it has an effect of improving periodontal disease and an effect of improving skin moisturizing function.

  Examples of formulations that can use the collagen production promoter of the present invention and periodontal disease improving agents are shown in Formulation Example 8 and examples of moisturizing cosmetics are shown in Formulation Example 9, but the present invention is not limited to these.

(Formulation example 1) Toothpaste
1.Dicalcium phosphate 40.0
2.Glycerin 20.0
3. Carrageenan 1.0
4.Sodium lauryl sulfate 1.5
5.Saccharin 0.2
6. Chlorhexidine digluconate 0.1
7. The defatted egoma extract obtained in Production Example 1 1.0
8. Preservative (butyl paraoxybenzoate) appropriate amount
9. Fragrance (Apple water)
10.Coloring agent appropriate amount
11. Residue with 100 purified water

(Formulation example 2) Mouth wash
1.Ibuprofen 0.1
2.Ethanol 10.0
3.Polyoxyethylene hydrogenated castor oil 0.5
4.Sodium lauryl sulfate 0.5
5.Saccharin sodium 0.5
6.Glycerin 10.0
7. The defatted egoma extract obtained in Production Example 1 1.0
8. Preservative (methylparaben) appropriate amount
9. Perfume (lime water) appropriate amount
10.Coloring agent appropriate amount
11. Residue with 100 purified water

(Formulation example 3) Mouth freshener
1.Ethanol 20.0
2.Glycerin 15.0
3. Polyoxyethylene hydrogenated castor oil 1.0
4.Saccharin 0.2
5.Chlorohexidine 0.01
6. The defatted egoma extract obtained in Production Example 1 1.0
7. Grapefruit fruit hot water extract 0.5
8. Preservative (methylparaben) appropriate amount
9.Coloring agent appropriate amount
10. Residue with 100 purified water

(Formulation example 4) Chewing gum mass%
1.Plate gum base 24.0
2. Minamata 13.0
3.Powdered sugar 60.0
4. The defatted egoma extract obtained in Production Example 1 1.0
5. Preservative (methyl paraben) appropriate amount
6. Fragrance (Peppermint water)
7. Residue with 100 purified water

(Formulation example 5) Candy mass%
1.Granulated sugar 45.0
2.Water tank 41.0
3. Powdered sugar 6.0
4. The defatted egoma extract obtained in Production Example 1 1.0
5. Preservative (methyl paraben) appropriate amount
6. Fragrance (Apple water)
7. Residue with 100 purified water

(Formulation Example 6) Milk Mass%
1.Squaric acid-2-magnesium phosphate 4.0
2.Squalane 5.0
3.Olive oil 5.0
4. Jojoba oil 5.0
5.Cetyl alcohol 1.5
6. Glycerin monostearate 2.0
7.Polyoxyethylene (20) cetyl ether 3.0
8.Polyoxyethylene (20) soorbitan monooleate 2.0
9.1,3-Butylene glycol 1.0
10.Glycerin 2.0
11. The defatted egoma extract obtained in Production Example 1 1.0
12.Paraoxybenzoic acid ester
13.Perfume appropriate amount
14. Residue with 100 purified water

(Prescription Example 7) Lotion Mass%
1.Squarylaminopropyl phosphate 3.0
2.Sodium glutamate 1.0
3.1,3-Butylene glycol 6.0
4.Glycerin 5.0
5.Polyethylene glycol 400 3.0
6.Olive oil 0.5
7.Polyoxyethylene sorbitan monostearate 1.5
8.Polyoxyethylene (5) oleyl alcohol ether 0.3
9.Ethanol 10.0
10. The defatted egoma extract obtained in Production Example 1 1.0
11.Perfume appropriate amount
12.Dye proper amount
13.Phenoxyethanol 0.1
14.Citric acid 1.0
15.Sodium citrate 1.2
16.Residue with 100 purified water

(Prescription Example 8) Periodontal disease improving dentifrice
1.Lactoferrin 2.0
2. The defatted egoma extract obtained in Production Example 1 1.0
3.Sorbitol 10.0
4.Sodium lauryl sulfate 1.0
5.Sodium alginate 0.5
6. Sodium polyacrylate 0.3
7.Propylene glycol 3.0
8.Saccharin sodium 0.01
9. Fragrance 0.15
10.Methylparaben 0.3
11. Residue with 100 purified water

(Formulation example 9) Moisturizing lotion
1. Cyclohexane-1,4-dicarboxylic acid bisethoxydiglycol
1.0
2.Trimethylglycine 2.0
3.Glycerin 4.0
4. 1,2-pentanediol 0.5
5. Polyethylene glycol 20000 0.5
6. Hydroxylated soybean lecithin 1.0
7. Glycosyl trehalose / hydrogenated starch degradation product mixed solution 1.0
8. Polyoxyethylene hydrogenated castor oil (60 EO) 0.2
9. The defatted egoma extract obtained in Production Example 1 1.0
10.Ethanol 4.0
11. Residue with 100 purified water

  The present invention finds that a defatted egoma extract promotes collagen production, and the defatted egoma can be applied as a periodontal disease improving agent and a moisturizing cosmetic.

Claims (3)

Collagen production promoter containing defatted egoma extract as an active ingredient The periodontal disease improving agent which mix | blends the collagen production promoter of Claim 1 as an active ingredient A moisturizing cosmetic comprising the collagen production promoter of claim 1 as an active ingredient.

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