JP2018074915A - Fermented milk containing milk protein concentrate as the main raw material, and method for producing the same - Google Patents
Fermented milk containing milk protein concentrate as the main raw material, and method for producing the same Download PDFInfo
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本発明は、液状の乳タンパク質濃縮物(MPC)を主原料とした、滑らかな食感を有する濃厚な発酵乳およびその製造方法に関する。 The present invention relates to a concentrated fermented milk having a smooth texture using a liquid milk protein concentrate (MPC) as a main raw material and a method for producing the same.
発酵乳は広く一般的に食される健康食品であり、需要者の嗜好の多様化に伴い、多様な発酵乳の需要が存在し、なかでも、濃厚な味わいを付与した発酵乳の需要は増加している。濃厚な味わいを付与した発酵乳の製造方法として、原料乳への増粘剤などの添加により、発酵乳に粘性を付与する方法(特許文献1)や、遠心分離法、水きり法などにより、発酵乳を濃縮する方法(特許文献2)が知られている。 Fermented milk is a health food that is widely eaten, and with the diversification of consumer preferences, there is a variety of demand for fermented milk. Among them, demand for fermented milk with a rich flavor is increasing. doing. As a method for producing fermented milk with a rich taste, fermentation can be performed by adding viscosity to fermented milk by adding a thickener or the like to the raw milk (Patent Document 1), centrifugation, draining, etc. A method for concentrating milk (Patent Document 2) is known.
一方、濃厚な味わいを付与した発酵乳を製造する方法として、限外ろ過処理によって濃縮した高タンパク質の原料乳を用いて発酵させる方法が開示されている(特許文献3)。ただし、原料乳の濃縮倍率が低いため、かかる発酵乳は濃厚さにおいて十分ではなく、MPCに特有のパサつきの問題は発生していない。また、濃厚な味わいを付与した発酵乳を製造する方法として、ナノろ過処理によって濃縮した所定の成分のMPCを含む原料乳を用いて発酵させる方法が開示されている(特許文献4)。ただし、MPCを含む原料乳にクリームを添加して、かかる発酵乳は食感の滑らかさを引き出している。 On the other hand, as a method for producing fermented milk imparted with a rich taste, a method of fermenting using high-protein raw material milk concentrated by ultrafiltration is disclosed (Patent Document 3). However, since the concentration ratio of the raw milk is low, the fermented milk is not sufficient in concentration, and the problem of dryness peculiar to MPC does not occur. Moreover, the method of fermenting using the raw material milk containing MPC of the predetermined | prescribed component concentrated by the nanofiltration process is disclosed as a method of manufacturing fermented milk provided with the rich taste (patent document 4). However, cream is added to raw material milk containing MPC, and such fermented milk draws the smoothness of texture.
しかしながら、原料乳に増粘剤などを添加する方法では、発酵乳の風味を損なう恐れがあり、また、増粘剤などの添加物を含まない食品への需要が高まっており、必ずしも好ましいとは言えない。また、遠心分離法、水切り法などにより、発酵乳を濃縮する方法では、ホエイが排出されるため、その処理が課題となる。 However, the method of adding a thickener or the like to raw milk may impair the flavor of fermented milk, and there is an increasing demand for foods that do not contain additives such as a thickener. I can not say. Moreover, in the method of concentrating fermented milk by a centrifugal separation method, a draining method, etc., since whey is discharged | emitted, the process becomes a subject.
本発明者らは、従来よりも高濃度のMPCを用いて発酵乳を製造した場合、かかる発酵乳の味わいは濃厚となるものの、パサつきの問題が発生することを見出した。また、粉末状のMPCを原料として発酵乳を製造した場合、粉末状のMPCの製造工程によって発生する加熱臭などにより、乳本来の味わいが損なわれる問題が発生しており、これらの理由から、十分に満足できる風味や品質を有する、高濃度のMPCを用いる発酵乳が得られていなかった。 The present inventors have found that when fermented milk is produced using a higher concentration of MPC than in the past, the taste of such fermented milk becomes thick, but a problem with dryness occurs. In addition, when fermented milk is produced using powdered MPC as a raw material, there is a problem that the original taste of milk is impaired due to a heated odor generated by the production process of powdered MPC. For these reasons, Fermented milk using a high concentration of MPC having a sufficiently satisfying flavor and quality has not been obtained.
本発明は、高タンパク質でありながら、滑らかな食感を有する濃厚な発酵乳を合理的に製造することを課題とし、かかる発酵乳では従来、パサつきの問題があり、十分に満足できる風味や食感が得られていなかった。 An object of the present invention is to rationally produce a concentrated fermented milk having a smooth texture while being high in protein, and such fermented milk has conventionally had a problem with dryness and has a sufficiently satisfactory flavor and food. The feeling was not obtained.
本発明者らは、液状のMPCが有する良質な乳風味に着目し、これを発酵乳に応用することにより、乳本来の味わいを有する濃厚な発酵乳を提供できるとの観点から、液状のMPCを主原料とした、高濃度のMPCを用いる発酵乳について研究を進めた。さらに、液状のMPCに、所定の糖類を配合して発酵させることで、増粘剤などの添加物やクリームなどの脂肪分を添加することなく、滑らかな食感を有する発酵乳が得られることを見出し、本発明を完成させるに至った。 The present inventors pay attention to the high-quality milk flavor of liquid MPC, and by applying this to fermented milk, liquid MPC can be provided from the viewpoint that concentrated fermented milk having the original taste of milk can be provided. Research was conducted on fermented milk using high-concentration MPC, which is mainly used as a raw material. Furthermore, fermented milk having a smooth texture can be obtained without adding additives such as thickeners and fats such as creams by blending and fermenting predetermined saccharides with liquid MPC. As a result, the present invention has been completed.
すなわち、本発明は、以下に関する。
[1] 原料乳を濃縮し、液状のMPCを得ること、
液状のMPCならびに、単糖類および二糖類からなる群から選択される1または2以上の糖類を配合(添加)してなる調合乳を調製すること、および
調合乳を発酵することを含む、発酵乳の製造方法。
[2] 調合乳のタンパク質の濃度が、7.5〜20重量%である、前記[1]に記載の発酵乳の製造方法。
[3] 限外ろ過(UF)処理により、原料乳を濃縮することを含む、前記[1]または[2]に記載の発酵乳の製造方法。
[4] 原料乳を濃縮する前に、原料乳を酸性化処理することをさらに含む、前記[1]〜[3]のいずれかに記載の発酵乳の製造方法。
[5] 液状のMPCに水を添加し、限外ろ過処理することをさらに含む、前記[3]または[4]に記載の発酵乳の製造方法。
[6] 調合乳を発酵する前に、液状のMPCを中和(中性化)処理することをさらに含む、前記[3]〜[5]のいずれかに記載の発酵乳の製造方法。
[7] 糖類が、ブドウ糖、ショ糖または乳糖である、前記[1]〜[6]のいずれかに記載の発酵乳の製造方法。
[8] 糖類の配合濃度が、1〜18重量%である、前記[1]〜[7]のいずれかに記載の発酵乳の製造方法。
[9] 前記[1]〜[8]のいずれかに記載の発酵乳の製造方法により製造された発酵乳。
That is, the present invention relates to the following.
[1] Concentrating raw milk to obtain liquid MPC,
Fermented milk, comprising preparing a formula comprising a liquid MPC and one or more saccharides selected from the group consisting of monosaccharides and disaccharides (added), and fermenting the formula Manufacturing method.
[2] The method for producing fermented milk according to [1], wherein the protein concentration of the formula milk is 7.5 to 20% by weight.
[3] The method for producing fermented milk according to the above [1] or [2], comprising concentrating raw material milk by ultrafiltration (UF) treatment.
[4] The method for producing fermented milk according to any one of [1] to [3], further comprising acidifying the raw milk before concentrating the raw milk.
[5] The method for producing fermented milk according to [3] or [4], further comprising adding water to liquid MPC and performing ultrafiltration.
[6] The method for producing fermented milk according to any one of [3] to [5], further comprising neutralizing (neutralizing) liquid MPC before fermenting the formula.
[7] The method for producing fermented milk according to any one of [1] to [6], wherein the saccharide is glucose, sucrose, or lactose.
[8] The method for producing fermented milk according to any one of [1] to [7], wherein the compounding concentration of the saccharide is 1 to 18% by weight.
[9] Fermented milk produced by the method for producing fermented milk according to any one of [1] to [8].
本発明は、液状のMPCを主原料とした、滑らかな食感を有する濃厚な発酵乳を合理的に製造する方法、および、そのようにして製造された発酵乳を提供することにある。 An object of the present invention is to provide a method for rationally producing concentrated fermented milk having a smooth texture using liquid MPC as a main raw material, and the fermented milk thus produced.
本発明において「発酵乳」とは、乳及び乳製品の成分規格等に関する省令(乳等省令)で定義される「発酵乳」を包含する。例えば、本発明において、発酵乳は、生乳、牛乳、特別牛乳、生山羊乳、殺菌山羊乳、生めん羊乳、成分調整牛乳、低脂肪牛乳、無脂肪牛乳および加工乳などの乳または、これと同等以上の無脂乳固形分を含む乳等を乳酸菌または酵母で発酵させ、糊状または液状にしたもの、または、これらを凍結したものをいう。これらは多様なヨーグルトを包含し、例えば、セットタイプヨーグルト(ハードヨーグルト)、ソフトヨーグルト、ドリンクヨーグルト、フローズンヨーグルトである。すなわち、本発明では、上記の乳等を濃縮し、発酵乳の原料となる調合乳を調製して、調合乳を発酵することで、高濃度の発酵乳を製造する。 In the present invention, “fermented milk” includes “fermented milk” defined by a ministerial ordinance (milk ordinance ordinance) relating to component specifications of milk and dairy products. For example, in the present invention, fermented milk is milk such as raw milk, milk, special milk, raw goat milk, pasteurized goat milk, raw noodle milk, ingredient-adjusted milk, low-fat milk, non-fat milk, processed milk, and the like. Milk or the like containing non-fat milk solid content equal to or higher than that is fermented with lactic acid bacteria or yeast to make a paste or liquid, or those frozen. These include various yogurts, for example, set type yogurt (hard yogurt), soft yogurt, drink yogurt, frozen yogurt. That is, in this invention, said milk | milk etc. are concentrated, the formula milk used as the raw material of fermented milk is prepared, and fermented milk of high concentration is manufactured by fermenting formula milk.
一般的に、プレーンヨーグルトなどのセットタイプヨーグルトは、調合乳を容器に充填し、その後に発酵すること(後発酵)により製造される。一方、ソフトヨーグルトやドリンクヨーグルトは、調合乳をタンク内等で発酵し、その後に微粒化処理や均質化処理してから容器に充填すること(前発酵)により製造される。 In general, set-type yogurt such as plain yogurt is manufactured by filling formulated milk in a container and then fermenting (post-fermentation). On the other hand, soft yogurt and drink yogurt are produced by fermenting formula milk in a tank or the like, and then filling the container (pre-fermentation) after atomization or homogenization.
本発明において、原料乳を高濃度(高タンパク質)で配合し、これを発酵させて濃厚な発酵乳を製造するため、遠心分離法、水切り法のような従来法では提供できない、セットタイプヨーグルト(後発酵のヨーグルト)を提供することもできる。 In the present invention, since raw milk is blended at a high concentration (high protein) and fermented to produce a concentrated fermented milk, a set type yogurt (cannot be provided by conventional methods such as a centrifugal separation method and a draining method) Post-fermented yogurt) can also be provided.
本発明において「原料乳」とは、液状のMPCの原料となるものであり、公知の原料乳を適宜使用することができる。本発明において、原料乳とは、殺菌前のものでも、殺菌後のものでもよい。本発明において、原料乳には、例えば、生乳、殺菌処理した乳、脱脂乳などを含んでおり、好ましくは、脱脂乳のみを含んでいる。 In the present invention, “raw material milk” is a raw material for liquid MPC, and known raw material milk can be used as appropriate. In the present invention, the raw milk may be either before sterilization or after sterilization. In the present invention, the raw milk contains, for example, raw milk, pasteurized milk, skim milk, etc., preferably only skim milk.
本発明において、原料乳を濃縮する方法は、好ましくは、乳本来の栄養成分や風味を損ないにくい、非加熱的な方法であり、原料乳から灰分および乳糖を低減(除去)できる観点およびタンパク質を濃縮できる観点から、より好ましくは、限外ろ過(UF)処理である。本発明において、限外ろ過(UF)膜の分画分子量は、とくに限定されず、例えば8000〜12000Da、好ましくは9000〜11000Da、より好ましくは9500〜10500Daである。本発明において、原料乳をさらに濃縮する方法は、原料乳の固形分を濃縮できる観点から、限外ろ過処理に加えて、逆浸透(RO)処理、ナノろ過(NF)処理、凍結濃縮処理、減圧蒸発濃縮処理などを適宜組み合わせてもよいが、非加熱的な方法である観点から、好ましくは、逆浸透(RO)処理、ナノろ過(NF)処理、凍結濃縮処理である。 In the present invention, the method for concentrating raw milk is preferably a non-heated method that does not easily impair the original nutritional components and flavor of milk, and is capable of reducing (removing) ash and lactose from raw milk. From the viewpoint of concentrating, ultrafiltration (UF) treatment is more preferable. In the present invention, the molecular weight cutoff of the ultrafiltration (UF) membrane is not particularly limited, and is, for example, 8000 to 12000 Da, preferably 9000 to 11000 Da, and more preferably 9500 to 10500 Da. In the present invention, the method of further concentrating the raw milk is a reverse osmosis (RO) process, a nanofiltration (NF) process, a freeze concentration process, in addition to the ultrafiltration process, from the viewpoint that the solid content of the raw material milk can be concentrated. A vacuum evaporation concentration treatment or the like may be appropriately combined, but from the viewpoint of a non-heating method, a reverse osmosis (RO) treatment, a nanofiltration (NF) treatment, and a freeze concentration treatment are preferable.
本発明において「液状のMPC(乳タンパク質濃縮物)」とは、原料乳を液状のままで濃縮して調製した乳タンパク質濃縮物であり、好ましくは、粉末状のMPCを還元して調製した液状のMPCを含まず、乳タンパク質を全固形分に対して60〜80重量%で含む液体である。本発明において、液状のMPCとは、好ましくは、限外ろ過処理により、原料乳から灰分および乳糖を除去しつつ、乳タンパク質を濃縮することで得られる液体である。かかる液体の乳タンパク質の構成比は、カゼイン:ホエイタンパク質の重量比として、好ましくは9:1〜7:3、より好ましくは8:2である。一般的に、乳タンパク質濃縮物は、MPC(Milk Protein Concentrate)または、TMP(Total Milk Protein)と呼ばれており、本発明では、これらを総称して「MPC」と記す。 In the present invention, “liquid MPC (milk protein concentrate)” is a milk protein concentrate prepared by concentrating raw milk in a liquid state, preferably a liquid prepared by reducing powdered MPC. This is a liquid containing milk protein in an amount of 60 to 80% by weight based on the total solid content. In the present invention, the liquid MPC is preferably a liquid obtained by concentrating milk protein while removing ash and lactose from raw milk by ultrafiltration. The composition ratio of the liquid milk protein is preferably 9: 1 to 7: 3, more preferably 8: 2, as the weight ratio of casein: whey protein. The milk protein concentrate is generally called MPC (Milk Protein Concentrate) or TMP (Total Milk Protein), and in the present invention, these are collectively referred to as “MPC”.
本発明において「調合乳」とは、液状のMPCを含む組成物である。本発明において、調合乳には、液状のMPCならびに、単糖類および/または二糖類の他に、例えば、水、脂質、タンパク質、香味成分、香料、色素、ミネラル(塩類)、ビタミン、甘味料、安定化剤、および、その他の食品用の添加物などを含んでもよい。本発明の一態様において、風味や食感が自然な発酵乳を製造する観点から、調合乳には、安定化剤、添加物を含まない。本発明の一態様において、健康志向の発酵乳を製造する観点から、脂質の濃度は、調合乳に対して、例えば3重量%以下(すなわち、0〜3重量%)、好ましくは2重量%以下(すなわち、0〜2重量%)、より好ましくは1重量%以下(すなわち、0〜1重量%)、さらに好ましくは実質的に0重量%である(脂質の濃度の分析値が0.5重量%以下であり、調合乳に脂質を配合しない)。本発明の一態様において、原料乳における脂肪の濃度を上記の数値に設定することで、低脂肪・無脂肪の発酵乳を提供することができる。 In the present invention, the “formulated milk” is a composition containing liquid MPC. In the present invention, in addition to liquid MPC and monosaccharide and / or disaccharide, for example, water, lipid, protein, flavor component, flavor, pigment, mineral (salt), vitamin, sweetener, Stabilizers and other food additives may also be included. In one embodiment of the present invention, from the viewpoint of producing fermented milk having a natural flavor and texture, the formula does not contain a stabilizer or an additive. In one embodiment of the present invention, from the viewpoint of producing health-oriented fermented milk, the lipid concentration is, for example, 3% by weight or less (that is, 0 to 3% by weight), preferably 2% by weight or less, based on the formula. (That is, 0 to 2% by weight), more preferably 1% by weight or less (that is, 0 to 1% by weight), and even more preferably substantially 0% by weight (the analytical value of the lipid concentration is 0.5% by weight). % Or less, and no lipid is added to the formula). In one embodiment of the present invention, low-fat and non-fat fermented milk can be provided by setting the fat concentration in the raw milk to the above-mentioned numerical values.
本発明の一態様において、濃厚な発酵乳を製造する観点から、タンパク質の濃度は、調合乳に対して、好ましくは7.5〜20重量%、より好ましくは8〜20重量%、さらに好ましくは8.5〜20重量%、さらに好ましくは9〜20重量%、さらに好ましくは9.5〜20重量%である。本発明の一態様において、滑らかな食感を有する発酵乳を製造する観点から、タンパク質の濃度は、調合乳に対して、好ましくは7.5〜16重量%、より好ましくは7.5〜15重量%、さらに好ましくは7.5〜14重量%、さらに好ましくは7.5〜13重量%、さらに好ましくは7.5〜12重量%である。本発明の一態様において、調合乳の熱安定性の観点から、タンパク質の含有濃度は、調合乳に対して、好ましくは7.5〜13重量%、より好ましくは7.5〜12.5重量%、さらに好ましくは7.5〜12重量%、さらに好ましくは7.5〜11.5重量%、さらに好ましくは7.5〜11重量%である。本発明の一態様において、滑らかな食感を有する濃厚な発酵乳を製造する観点から、タンパク質の濃度は、調合乳に対して、好ましくは7.5〜16重量%、より好ましくは8〜15重量%、さらに好ましくは8.5〜14重量%、さらに好ましくは9〜13重量%、さらに好ましくは9.5〜12重量%である。 In one embodiment of the present invention, from the viewpoint of producing concentrated fermented milk, the protein concentration is preferably 7.5 to 20% by weight, more preferably 8 to 20% by weight, still more preferably, based on the formula. It is 8.5 to 20 weight%, More preferably, it is 9 to 20 weight%, More preferably, it is 9.5 to 20 weight%. In one embodiment of the present invention, from the viewpoint of producing fermented milk having a smooth texture, the protein concentration is preferably 7.5 to 16% by weight, more preferably 7.5 to 15%, based on the formula. % By weight, more preferably 7.5 to 14% by weight, still more preferably 7.5 to 13% by weight, and still more preferably 7.5 to 12% by weight. In one embodiment of the present invention, from the viewpoint of heat stability of the formula, the protein concentration is preferably 7.5 to 13% by weight, more preferably 7.5 to 12.5% by weight based on the formula. %, More preferably 7.5 to 12% by weight, still more preferably 7.5 to 11.5% by weight, and still more preferably 7.5 to 11% by weight. In one embodiment of the present invention, from the viewpoint of producing a concentrated fermented milk having a smooth texture, the protein concentration is preferably 7.5 to 16% by weight, more preferably 8 to 15%, based on the formula. % By weight, more preferably 8.5 to 14% by weight, still more preferably 9 to 13% by weight, and still more preferably 9.5 to 12% by weight.
本発明の一態様において、調合乳のタンパク質は、主に、液状のMPCに由来し、カゼイン、ホエイタンパク質(α-ラクトアルブミン、β-ラクトグロブリンなど)などを含む。本発明の一態様において、調合乳は、液状のMPCを主原料として含む。ここで、主原料とは、調合乳に最も多く含有される原料であり、例えば、9割以上、8割以上または7割以上で含有される原料である。 In one embodiment of the present invention, the protein of the formula is mainly derived from liquid MPC and includes casein, whey protein (α-lactalbumin, β-lactoglobulin, etc.) and the like. In one embodiment of the present invention, the formula contains liquid MPC as a main raw material. Here, the main raw material is a raw material contained most in the formula milk, for example, a raw material contained in 90% or more, 80% or more, or 70% or more.
本発明の一態様において、発酵乳の過剰な塩味や、えぐ味を低減する観点、および発酵乳の適度なコク味を付与する観点から、灰分の濃度は、調合乳に対して、好ましくは0.15〜2重量%、より好ましくは0.2〜1.8重量%、さらに好ましくは0.25〜1.6重量%、さらに好ましくは0.3〜1.4重量%である。本発明の一態様において、発酵乳の過剰な塩味や、えぐ味を低減する観点、および発酵乳の適度なコク味を付与する観点から、カルシウムの濃度は、調合乳に対して、好ましくは0.05〜0.7重量%、より好ましくは0.05〜0.6重量%、さらに好ましくは0.1〜0.5重量%。さらに好ましくは0.1〜0.4重量%である。本発明の一態様において、調合乳の灰分は、主に、液状のMPCに由来し、ナトリウム、カリウム、マグネシウム、カルシウム、リン、塩素などを含む。 In one embodiment of the present invention, the concentration of ash is preferably 0 with respect to the formula from the viewpoint of reducing the excessive salty taste and fermented taste of fermented milk and imparting an appropriate rich taste of fermented milk. .15 to 2% by weight, more preferably 0.2 to 1.8% by weight, still more preferably 0.25 to 1.6% by weight, still more preferably 0.3 to 1.4% by weight. In one embodiment of the present invention, the concentration of calcium is preferably 0 with respect to the formula from the viewpoint of reducing excessive salty taste and fermented taste of fermented milk and imparting a suitable rich taste of fermented milk. 0.05 to 0.7% by weight, more preferably 0.05 to 0.6% by weight, still more preferably 0.1 to 0.5% by weight. More preferably, it is 0.1 to 0.4% by weight. In one embodiment of the present invention, the ash content of the formula is mainly derived from liquid MPC and contains sodium, potassium, magnesium, calcium, phosphorus, chlorine, and the like.
本発明において、食品に通常で用いられる乳酸菌を調合乳に添加(配合)して、調合乳を発酵させることができる。本発明において、食品に通常で用いられる乳酸菌は、好ましくは、ブルガリア菌(Lactobacillus bulgaricus)、サーモフィラス菌(Streptococcus thermophiles)、ガセリ菌(Lactobacillus gasseri)からなる群から選択される1または2以上の乳酸菌、より好ましくは、ブルガリア菌およびサーモフィラス菌の組み合わせである。すなわち、本発明において、コーデックス規格でヨーグルトスターターとして規格化されているブルガリア菌およびサーモフィラス菌の混合スターターを好適に用いることができる。本発明において、この混合スターター(ヨーグルトスターター)を用いて、発酵条件(発酵温度、発酵時間など)を勘案した上で、さらに、食品に通常で用いられるビフィズス菌、プロピオン酸菌、酵母などの有用な微生物を調合乳に添加してもよい。また、本発明において、食品に通常で用いられるビフィズス菌、プロピオン酸菌、酵母などの有用な微生物を調合乳に添加してもよい。 In the present invention, the lactic acid bacteria usually used in foods can be added (blended) to the formula to ferment the formula. In the present invention, the lactic acid bacteria usually used in foods are preferably one or more lactic acid bacteria selected from the group consisting of Bulgarian bacteria (Lactobacillus bulgaricus), Thermophilus bacteria (Streptococcus thermophiles), Gasseri bacteria (Lactobacillus gasseri), More preferred is a combination of Bulgarian bacteria and Thermophilus bacteria. That is, in the present invention, a mixed starter of Bulgarian bacteria and Thermophilus bacteria standardized as a yogurt starter in the Codex standard can be suitably used. In the present invention, this mixed starter (yoghurt starter) is used in consideration of fermentation conditions (fermentation temperature, fermentation time, etc.), and further useful for bifidobacteria, propionic acid bacteria, yeast, etc. that are usually used in foods. New microorganisms may be added to the formula. In the present invention, useful microorganisms such as bifidobacteria, propionic acid bacteria, and yeast that are commonly used in foods may be added to the formula.
本発明において、調合乳の発酵では、通常の発酵乳の製造と同様の条件を設定することができる。本発明において、調合乳の発酵温度を適宜設定すればよく、好ましくは30〜45℃、より好ましくは35〜44℃、さらに好ましくは37〜43℃に設定することができる。本発明において、調合乳の発酵時間を適宜設定すればよく、好ましくは1〜36時間、より好ましくは2〜24時間、さらに好ましくは3〜12時間、さらに好ましくは4〜8時間に設定することができる。 In the present invention, the same conditions as in the production of normal fermented milk can be set for fermented formula. In this invention, what is necessary is just to set the fermentation temperature of formula milk suitably, Preferably it is 30-45 degreeC, More preferably, it is 35-44 degreeC, More preferably, it can set to 37-43 degreeC. In this invention, what is necessary is just to set the fermentation time of formula milk suitably, Preferably it is 1-36 hours, More preferably, it is 2-24 hours, More preferably, it is 3-12 hours, More preferably, it should set to 4-8 hours. Can do.
本発明において、調合乳の加熱殺菌処理では、通常の発酵乳の製造と同様の条件を設定することができる。本発明において、調合乳の加熱殺菌温度と加熱殺菌時間の組合せを適宜設定すればよく、例えば、規格化または汎用されている加熱殺菌条件として、75℃・達温、95℃・達温、75℃〜95℃・15〜60秒間、110℃〜150℃・1〜5秒間に設定することができる。本発明の一態様において、滑らかな食感を有する発酵乳を製造する観点から、調合乳の加熱殺菌温度と加熱殺菌時間の組合せを、好ましくは110℃〜130℃・2〜4秒間に設定することができる。 In the present invention, in the heat sterilization treatment of formula milk, the same conditions as in the production of normal fermented milk can be set. In the present invention, the combination of the heat sterilization temperature and the heat sterilization time of the formula may be set as appropriate. For example, as standardized or widely used heat sterilization conditions, 75 ° C./attainment temperature, 95 ° C./attainment temperature, 75 The temperature can be set to 110 ° C. to 95 ° C. for 15 to 60 seconds and 110 ° C. to 150 ° C. for 1 to 5 seconds. In one aspect of the present invention, from the viewpoint of producing fermented milk having a smooth texture, the combination of the heat sterilization temperature and the heat sterilization time of the formula is preferably set to 110 ° C. to 130 ° C. · 2 to 4 seconds. be able to.
本発明において、液状のMPCならびに、単糖類および二糖類からなる群から選択される1または2以上の糖類を配合してなる調合乳を調製する。本発明において、単糖類および二糖類は、とくに限定されないが、風味や食感が良好な発酵乳を製造する観点から、単糖類は、例えば、ブドウ糖、マンノース、アラビノース、フルクトースなどであり、二糖類は、例えば、ショ糖、乳糖、麦芽糖、トレハロース、ラクツロースなどであり、単糖類は、好ましくは、ブドウ糖であり、二糖類は、好ましくは、ショ糖、乳糖である。かかる糖類は、増粘剤などの添加物やクリームなどの脂肪分を添加することなく、MPCを主原料とした発酵乳の食感を滑らかにする効果を有する。 In the present invention, a formula comprising a liquid MPC and one or more saccharides selected from the group consisting of monosaccharides and disaccharides is prepared. In the present invention, monosaccharides and disaccharides are not particularly limited, but monosaccharides are, for example, glucose, mannose, arabinose, fructose and the like from the viewpoint of producing fermented milk having a good flavor and texture. Is, for example, sucrose, lactose, maltose, trehalose, lactulose, the monosaccharide is preferably glucose, and the disaccharide is preferably sucrose or lactose. Such saccharides have the effect of smoothing the texture of fermented milk made mainly of MPC without adding additives such as thickeners and fats such as cream.
本発明において、滑らかな食感を有する発酵乳を製造する観点から、調合乳の調製に際して、単糖類および二糖類からなる群から選択される1または2以上の糖類の配合(添加)濃度は、調合乳に対して、例えば1〜18重量%、好ましくは1.5〜18重量%、より好ましくは1.5〜17重量%、さらに好ましくは1.5〜15重量%、さらに好ましくは1.5〜12重量%、さらに好ましくは1.5〜10重量%、さらに好ましくは2〜10重量%、さらに好ましくは2〜8重量%、さらに好ましくは2.5〜8重量%、さらに好ましくは2.5〜6重量%、さらに好ましくは3〜6重量%、さらに好ましくは3〜5重量%である。本発明において、例えば、ブドウ糖、ショ糖および乳糖からなる群から選択される1または2以上の糖類の総量として、前記の濃度の範囲になるように、糖類を配合すればよい。本発明の一態様において、ブドウ糖、ショ糖または乳糖の配合濃度は、調合乳に対して、例えば1〜18重量%、好ましくは1.5〜18重量%、より好ましくは1.5〜17重量%、さらに好ましくは1.5〜15重量%、さらに好ましくは1.5〜12重量%、さらに好ましくは1.5〜10重量%、さらに好ましくは2〜10重量%、さらに好ましくは2〜8重量%、さらに好ましくは2.5〜8重量%、さらに好ましくは2.5〜6重量%、さらに好ましくは3〜6重量%、さらに好ましくは3〜5重量%である。なお、前記の糖類の配合濃度に、液状のMPCに由来する糖類の濃度は含まれないものとする。 In the present invention, from the viewpoint of producing fermented milk having a smooth texture, when preparing the formula, the blending (addition) concentration of one or more saccharides selected from the group consisting of monosaccharides and disaccharides is: For example, 1 to 18% by weight, preferably 1.5 to 18% by weight, more preferably 1.5 to 17% by weight, still more preferably 1.5 to 15% by weight, and still more preferably 1. 5 to 12 wt%, more preferably 1.5 to 10 wt%, more preferably 2 to 10 wt%, more preferably 2 to 8 wt%, more preferably 2.5 to 8 wt%, more preferably 2 0.5 to 6% by weight, more preferably 3 to 6% by weight, and still more preferably 3 to 5% by weight. In the present invention, for example, saccharides may be blended so that the total amount of one or more saccharides selected from the group consisting of glucose, sucrose, and lactose is within the above-mentioned concentration range. In one embodiment of the present invention, the concentration of glucose, sucrose or lactose is, for example, 1 to 18% by weight, preferably 1.5 to 18% by weight, more preferably 1.5 to 17% by weight based on the formula. %, More preferably 1.5 to 15% by weight, more preferably 1.5 to 12% by weight, more preferably 1.5 to 10% by weight, still more preferably 2 to 10% by weight, more preferably 2 to 8%. % By weight, more preferably 2.5-8% by weight, more preferably 2.5-6% by weight, more preferably 3-6% by weight, and still more preferably 3-5% by weight. In addition, the density | concentration of the saccharide derived from liquid MPC is not included in the compounding density | concentration of the said saccharide | sugar.
本発明の一態様において、商業的に許容可能な発酵時間で制御する観点から、調合乳における乳糖の濃度は、3重量%以上、好ましくは3.2重量%以上、より好ましくは3.4重量%以上、さらに好ましくは3.6重量%以上、さらに好ましくは3.8重量%以上である。本発明の一態様において、商業的に許容可能な発酵時間で制御する観点から、調合乳における乳糖の濃度は、18重量%以下、好ましくは16重量%以下、より好ましくは14重量%以下、さらに好ましくは12重量%以下、さらに好ましくは10重量%以下、さらに好ましくは8重量%以下、さらに好ましくは6重量%以下、さらに好ましくは5重量%以下、さらに好ましくは4.8重量%以下である。なお、前記の乳糖の濃度に、液状のMPCに由来する乳糖の濃度は含まれるものとする。 In one embodiment of the present invention, from the viewpoint of controlling with a commercially acceptable fermentation time, the concentration of lactose in the formula is 3% by weight or more, preferably 3.2% by weight or more, more preferably 3.4% by weight. % Or more, more preferably 3.6% by weight or more, and still more preferably 3.8% by weight or more. In one embodiment of the present invention, the concentration of lactose in the formula is 18% by weight or less, preferably 16% by weight or less, more preferably 14% by weight or less, from the viewpoint of controlling with a commercially acceptable fermentation time. Preferably it is 12% by weight or less, more preferably 10% by weight or less, more preferably 8% by weight or less, more preferably 6% by weight or less, more preferably 5% by weight or less, and even more preferably 4.8% by weight or less. . The concentration of lactose derived from liquid MPC is included in the concentration of lactose.
本発明の一態様において、原料乳を濃縮する前に、原料乳を酸性化処理し、MPCから苦味の原因のカルシウム(Ca)を効率的に低減する(除去する)工程を含む。すなわち、本発明では、MPCからCaを低減するため、原料乳を濃縮する前に、原料乳のCaを可溶化させる酸性化処理を含むことができる。かかる酸性化処理は、Caを効率的に可溶化する観点および乳タンパク質を安定化する観点から、原料乳のpHを、例えば5.5〜6.5、好ましくは5.5〜6.2、より好ましくは5.5〜6.0、さらに好ましくは5.5〜5.8に調整し、例えば0.5時間以上、好ましくは1時間以上、より好ましくは1.5時間以上、さらに好ましくは2時間以上で保持する。かかる酸性化処理は、原料乳のpHを調整できれば、とくに限定されないが、例えば、塩酸、リン酸、クエン酸などを原料乳に添加する。 In one embodiment of the present invention, the method includes a step of acidifying the raw milk and efficiently reducing (removing) calcium (Ca) causing bitterness from the MPC before concentrating the raw milk. That is, in this invention, in order to reduce Ca from MPC, the acidification process which solubilizes Ca of raw material milk can be included before concentrating raw material milk. From the viewpoint of efficiently solubilizing Ca and stabilizing milk protein, the acidification treatment is performed by adjusting the pH of the raw milk to, for example, 5.5 to 6.5, preferably 5.5 to 6.2. More preferably, it is adjusted to 5.5 to 6.0, more preferably 5.5 to 5.8, for example, 0.5 hours or more, preferably 1 hour or more, more preferably 1.5 hours or more, further preferably Hold for at least 2 hours. Such acidification treatment is not particularly limited as long as the pH of the raw material milk can be adjusted. For example, hydrochloric acid, phosphoric acid, citric acid and the like are added to the raw material milk.
本発明の一態様において、液状のMPCに水を添加して、限外ろ過処理し、MPCから灰分および乳糖を効率的に低減する工程を含む。すなわち、本発明では、MPCから灰分および乳糖を低減するため、液状のMPCに加水する限外ろ過処理である透析ろ過(DF)処理を含むことができる。かかる透析ろ過処理は、液状のMPCから灰分および乳糖を効率的に低減(除去)できる観点およびタンパク質を効率的に濃縮できる観点から、複数回であってもよい。 In one embodiment of the present invention, the method includes a step of adding water to liquid MPC and performing ultrafiltration to efficiently reduce ash and lactose from MPC. That is, in the present invention, in order to reduce ash and lactose from MPC, a diafiltration (DF) process, which is an ultrafiltration process for adding water to liquid MPC, can be included. Such diafiltration may be performed a plurality of times from the viewpoint of efficiently reducing (removing) ash and lactose from liquid MPC and from the viewpoint of efficiently concentrating proteins.
本発明の一態様において、調合乳を発酵する前に、液状のMPCを中和(中性化)処理し、液状のMPCまたは調合乳の加熱殺菌処理における熱安定性を付与する工程を含む。すなわち、本発明では、液状のMPCまたは調合乳の加熱殺菌処理における熱安定性を付与するため、酸性化処理した液状のMPCの中和処理を含むことができる。かかる中和処理は、乳タンパク質を安定化する観点から、液状のMPCのpHを、例えば6.5〜7.0、好ましくは6.5〜6.9、より好ましくは6.5〜6.8、さらに好ましくは6.5〜6.7に調整する。 One embodiment of the present invention includes a step of neutralizing (neutralizing) liquid MPC before fermenting the formula and imparting heat stability in the heat sterilization process of the liquid MPC or formula. That is, in this invention, in order to provide the thermal stability in the heat sterilization process of liquid MPC or formula milk, the neutralization process of the acidified liquid MPC can be included. In the neutralization treatment, the pH of the liquid MPC is, for example, 6.5 to 7.0, preferably 6.5 to 6.9, more preferably 6.5 to 6. 8, More preferably, it is adjusted to 6.5 to 6.7.
以下に、本発明に係る方法を実施例に基づき、さらに詳しく説明するが、本発明は、これらに限定されるものではない。 Hereinafter, the method according to the present invention will be described in more detail based on examples, but the present invention is not limited thereto.
[実施例1]
(a1)液状のMPCの調製
生乳(原乳)を遠心分離処理(50℃)して、脱脂乳を調製し、この脱脂乳を冷却(5℃)した後に、リン酸を用いて、この脱脂乳のpHを5.7に調整して2時間で保持(酸性化処理)した。そして、この脱脂乳を限外ろ過(UF)処理(スパイラル型膜、Koch社 HpHT 3838−K131−NYV、分画分子量:10,000Da、膜面積:6.1m2×並列2本)し、濃縮倍率で3.2倍に濃縮して、濃縮液を調製した。そして、この濃縮液のpHを6.7に中和して調整し、液状の乳タンパク質濃縮物(MPC)を調製した。
[Example 1]
(A1) Preparation of liquid MPC Raw milk (raw milk) is centrifuged (50 ° C.) to prepare skim milk, and this skim milk is cooled (5 ° C.). The pH of the milk was adjusted to 5.7 and held for 2 hours (acidification treatment). Then, this skim milk is subjected to ultrafiltration (UF) treatment (spiral type membrane, Koch HpHT 3838-K131-NYV, molecular weight cut off: 10,000 Da, membrane area: 6.1 m 2 × 2 in parallel), and concentrated. The concentrate was concentrated by a factor of 3.2 to prepare a concentrate. And the pH of this concentrate was neutralized and adjusted to 6.7, and the liquid milk protein concentrate (MPC) was prepared.
(b1)糖類を配合した液状のMPCの発酵乳(ハードタイプ、ソフトタイプ)の製造
発明品として、(a1)で調製した液状のMPCに水を添加し、全固形分の濃度が15重量%、タンパク質の濃度が9.7重量%となるように調整し、これを3等分し、それぞれに、ブドウ糖を4重量%、ショ糖を4重量%または乳糖(無水)を3重量%で配合して、調合乳を調製した。そして、プレート式熱交換器(Power point International 社)を用いて、この調合乳を130℃、2秒間で連続式に加熱した後に、この調合乳を冷却(43℃)し、乳酸菌スターター(明治ブルガリアヨーグルトから分離した)を2重量%で添加してから、pHが4.6になるように発酵(43℃、340分間)させて、ハードタイプおよびソフトタイプの発酵乳を製造した。このとき、ソフトタイプの発酵乳は、ハードタイプの発酵乳をスリーワンモーターで攪拌してから、フィルター(孔径:250μm)を通過させて製造した。
(B1) Manufacture of fermented milk (hard type, soft type) of liquid MPC mixed with saccharide As an invention product, water is added to liquid MPC prepared in (a1), and the concentration of total solids is 15% by weight Adjust the protein concentration to 9.7% by weight and divide it into three equal parts, each containing 4% by weight glucose, 4% by weight sucrose or 3% by weight lactose (anhydrous). Thus, a formula was prepared. Then, using a plate-type heat exchanger (Power point International), this formula is heated continuously at 130 ° C. for 2 seconds, and then this formula is cooled (43 ° C.), and then a lactic acid bacteria starter (Meiji Bulgaria). (Separated from yogurt) was added at 2% by weight and then fermented (43 ° C., 340 minutes) to a pH of 4.6 to produce hard type and soft type fermented milk. At this time, the soft type fermented milk was produced by stirring the hard type fermented milk with a three-one motor and then passing it through a filter (pore size: 250 μm).
[比較例1]
(a2)液状のMPCの調製
実施例1の(a1)と同様にして、液状のMPCを調製した。
[Comparative Example 1]
(A2) Preparation of liquid MPC A liquid MPC was prepared in the same manner as in (a1) of Example 1.
(b2)糖無添加の液状のMPCの発酵乳(ハードタイプ、ソフトタイプ)の製造
対照(比較対照)品として、(a1)で調製した液状のMPCに水を添加し、全固形分の濃度が15重量%、タンパク質の濃度が9.7重量%となるように調整し、調合乳を調製した。そして、プレート式熱交換器を用いて、この調合乳を130℃、2秒間で連続式に加熱した後に、この調合乳を冷却(43℃)し、乳酸菌スターター(明治ブルガリアヨーグルトから分離した)を2重量%で添加してから、pHが4.6になるように発酵(43℃、340分間)させて、ハードタイプおよびソフトタイプの発酵乳を製造した。このとき、ソフトタイプの発酵乳は、ハードタイプの発酵乳をスリーワンモーターで攪拌してから、フィルター(孔径:250μm)を通過させて製造した。
(B2) Production of fermented milk (hard type, soft type) of liquid MPC without addition of sugar As a control (comparison control) product, water is added to the liquid MPC prepared in (a1), and the concentration of total solids Was adjusted to 15 wt% and the protein concentration was 9.7 wt% to prepare a formula. And after heating this formula milk continuously at 130 degreeC for 2 second using a plate-type heat exchanger, this formula milk is cooled (43 degreeC), Lactic acid bacteria starter (separated from Meiji Bulgaria yogurt) After adding at 2% by weight, fermentation was performed (43 ° C., 340 minutes) so that the pH was 4.6 to produce hard type and soft type fermented milk. At this time, the soft type fermented milk was produced by stirring the hard type fermented milk with a three-one motor and then passing it through a filter (pore size: 250 μm).
[試験例1]
(c)ハードタイプおよびソフトタイプの発酵乳の官能検査
発明品と対照品の発酵乳について、風味と食感を官能検査(専門パネル:10名、評価:5段階)した。ブドウ糖を配合したハードタイプおよびソフトタイプの発酵乳の官能検査の結果と、糖無添加のハードタイプおよびソフトタイプの発酵乳の官能検査の結果の比較図を、それぞれ図1および図2に示す。ショ糖を配合したハードタイプおよびソフトタイプの発酵乳の官能検査の結果と、糖無添加のハードタイプおよびソフトタイプの発酵乳の官能検査の結果の比較図を、それぞれ図3および図4に示す。乳糖を配合したハードタイプおよびソフトタイプの発酵乳の官能検査の結果と、糖無添加のハードタイプおよびソフトタイプの発酵乳の官能検査の結果の比較図を、それぞれ図5および図6に示す。
[Test Example 1]
(C) Sensory test of hard type and soft type fermented milk The sensory test of the flavor and texture of the fermented milk of the invention product and the control product (special panel: 10 persons, evaluation: 5 stages) A comparison diagram of the results of the sensory test of the hard type and soft type fermented milk blended with glucose and the result of the sensory test of the hard type and soft type fermented milk added with no sugar is shown in FIGS. 1 and 2, respectively. FIG. 3 and FIG. 4 show a comparison of the results of the sensory test of hard type and soft type fermented milk mixed with sucrose and the results of the sensory test of hard type and soft type fermented milk without addition of sugar, respectively. . FIG. 5 and FIG. 6 show a comparison diagram of the results of sensory test of hard type and soft type fermented milk mixed with lactose and the result of sensory test of hard type and soft type fermented milk without addition of sugar, respectively.
ブドウ糖を配合した発酵乳では、糖無添加の発酵乳に比べて、食感の滑らかさと甘味が向上され、えぐ味が低減された。ショ糖を配合した発酵乳では、糖無添加の発酵乳に比べて、食感の滑らかさと甘味が向上され、酸味とえぐ味が低減された。乳糖を配合した発酵乳では、糖無添加の発酵乳に比べて、食感の滑らかさと乳風味が向上され、えぐ味が低減された。 Compared with fermented milk without addition of sugar, the fermented milk blended with glucose improved the smoothness and sweetness of the texture and reduced the taste. The fermented milk blended with sucrose improved the smoothness and sweetness of the texture and reduced the acidity and gummy taste, compared to the fermented milk without sugar. In fermented milk blended with lactose, compared to fermented milk with no sugar added, the smoothness of the texture and the milk flavor were improved, and the gummy taste was reduced.
[試験例2]
(d)低カルシウムのMPCの調製
生乳(原乳)を遠心分離処理(50℃)して、脱脂乳を調製し、この脱脂乳を冷却(5℃)した後に、この脱脂乳のpHを未調整にするか(pHは6.7である)、塩酸を用いて、この脱脂乳のpHを6.1および5.7に調整して2時間で保持(酸性化処理)した。そして、この脱脂乳を、それぞれ限外ろ過(UF)処理(スパイラル型膜、Koch社 HpHT 3838−K131−NYV、分画分子量:10,000Da、膜面積:6.1m2×並列2本)し、濃縮倍率で4倍に濃縮して、濃縮液を調製した。そして、この濃縮液のpHを6.7に中和して調整し、液状のMPCを調製した。これらの液状のMPCの組成を表1に示す。
[Test Example 2]
(D) Preparation of low-calcium MPC Raw milk (raw milk) is centrifuged (50 ° C.) to prepare skim milk, and after this skim milk is cooled (5 ° C.), the pH of the skim milk is not adjusted. The pH of the skim milk was adjusted to 6.1 and 5.7 using hydrochloric acid, and maintained for 2 hours (acidification treatment). And this skim milk was each subjected to ultrafiltration (UF) treatment (spiral type membrane, Koch HpHT 3838-K131-NYV, molecular weight cut-off: 10,000 Da, membrane area: 6.1 m 2 × 2 in parallel). Then, the solution was concentrated 4 times at a concentration ratio to prepare a concentrated solution. And the pH of this concentrate was neutralized and adjusted to 6.7, and liquid MPC was prepared. Table 1 shows the composition of these liquid MPCs.
原料乳(脱脂乳)を酸性化処理して、原料乳のpHを変化させることにより、所望のCaの濃度を有するMPCを調製することができる。そして、このMPCを主原料として、発酵乳を製造することができる。 MPC having a desired Ca concentration can be prepared by acidifying raw milk (skim milk) and changing the pH of raw milk. And fermented milk can be manufactured by using this MPC as a main raw material.
本発明は、MPCを主原料とした、高タンパク質でありながら、滑らかな食感を有する濃厚な発酵乳を合理的に製造する方法、および、そのようにして製造された発酵乳を提供する。 The present invention provides a method for rationally producing concentrated fermented milk having a smooth texture while having a high protein, using MPC as a main raw material, and the fermented milk thus produced.
Claims (9)
液状のMPCならびに、単糖類および二糖類からなる群から選択される1または2以上の糖類を配合してなる調合乳を調製すること、および
調合乳を発酵することを含む、発酵乳の製造方法。 Concentrating raw milk to obtain liquid MPC;
A method for producing fermented milk, comprising preparing a formula comprising a liquid MPC and one or more saccharides selected from the group consisting of monosaccharides and disaccharides, and fermenting the formula .
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