JP2017222596A - Pesticide comprising microbial metabolite - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a control agent that exhibits excellent controlling effect against pest insects, nematodes and mites at low dosage, and yet, in addition, that is comparatively quick to decompose in the environment and does not exhibit cross-resistance to existing synthetic insecticides.SOLUTION: A control agent containing lenoremycin, which is an antibiotic from microbial metabolite, is used.EFFECT: The control agent exhibits excellent control effect against cotton aphid, Prodenia litura, Diamondback moth, Brown planthopper etc., mites such as two-spotted spider mite, and nematodes such as sweet potato root-knot nematode, which are pests in the fields of fruit trees, vegetables, and other crops as well as flowers.SELECTED DRAWING: None

Description

The present invention relates to useful insecticides containing microbial metabolites or salts thereof.

  Various microbial metabolites have been identified and studied so far for the purpose of utilizing microbial metabolites. In Non-Patent Documents 1 and 2, it is known that microbial metabolites (lenomycin) have antibiotic activity. . However, there is no description regarding insecticidal activity against pests in the fields of agriculture, veterinary drugs, quasi drugs and the like.

ANTIBIOTIC A-130, isolation and charactarization: K Tokyo, H Hiroshi, et al; Journal of Antibiotics (1975), 28 (12), 931-4 Studies on a new polytheantiantiotic, Ro 21-6150: L Chao-Min, E Ralph, et al; Journal of Antibiotics (1976), 29 (1), 21-8

In crop production such as agriculture, the damage caused by various pests is large, with low dosage for the purpose of generating resistance to existing drugs and reducing environmental burden, etc., and with relatively fast degradation in the environment, existing synthesis Development of novel insecticides using microbial metabolites that do not exhibit cross-resistance to insecticides is desired. An object of the present invention is to provide a microbial metabolite having insecticidal activity in order to meet these demands.

  As a result of intensive studies to solve the above problems, the present inventors have newly discovered that microbial metabolites (lenoremycin) have insecticidal activity.

」、に関する。 The present invention
“(1) Insecticide containing a microbial metabolite represented by formula (1) or a salt thereof

".

The microbial metabolite of the present invention exhibits an excellent insecticidal activity against pests, nematodes and spider mites, and exhibits an excellent control effect.

The microbial metabolite represented by the formula (1) of the present invention is available from the market.

The insecticide containing the microbial metabolite represented by the formula (1) of the present invention or a salt thereof can be used for pest control in fruit trees, vegetables, other crops, and flowering fields.

The pests to be used for the insecticide of the present invention are not particularly limited, and the insect species to be controlled in the present invention (the insect species in which the compound represented by the formula (1) exhibits a controlling effect) is not limited. It can be used to control various pests. Preferable species of insects to be controlled include, for example, lepidoptera pests (for example, moths such as Spodoptera litura, Shirochimojiyotou, Ayayoto, Yotoga, Tamanayaga, Trichopulsia, Heliotis spp, Helicoverpa sppp, etc .; Common moths, such as Japanese corn borer, European corn borer, European corn borer, Hydra corn borer, Shibatatsuga, Watanomeiga, Noshimemadaranoga, white butterflies such as white butterflies; Antelope moths; Limantria genus, Euproctinis genus moths, Saga moths, etc .; Cotton moths, etc .; Birds such as birds, leafhoppers, aphids such as peach aphids, cotton aphids; leafhoppers such as leafhoppers, leafhoppers, white-spotted leafhoppers; leafhoppers such as leafhoppers; Stink bugs such as red-bellied beetle, Chabanae stink bug, Minamia stink bug, Hoso-heli beetle, etc .; For example, weevil such as maize weevil, rice weevil, azuki bee weevil; beetle such as chrysanthemum beetle; beetle such as beetle moth, beetle; Potato beetles, western corn root worms, southern corn root worms, etc .; epidermis such as rice beetle, blue fly agaric beetle, euglena, nematode moth, etc .; Spider mites such as apple spider mite, oligonicus spp .; mites such as tomato spider mite, citrus spider mite, and chrysanthemum mite; dust mites such as mite dust mites; mite mites; etc .; Eye pests (eg, grasshoppers), diptera pests (eg, house flies; house moths; anopheles; chironomids; black flies; sand flies; sand flies; fly flies; legumes, tomatoes, tomatoes) D. Flies; flies; flies; Drosophila; butterflies; flyfish; fly species; fly flies; etc. Thrips, Thrips thrips, Thrips thrips, Thrips thrips, etc., plant parasitic nematodes (eg root-knot nematodes; Nepenthes nematodes; Cyst nematodes; Aferencoides, such as rice hump nematodes; Parasitic nematodes (for example, fecal nematodes; couperia; torsion stomachworms; whipworms; roundworms; nodules; More preferred are lepidopterous insects, semilepidopterous insects, Coleoptera insects, mite insects, diptera insects, thrips insects, or plant parasitic nematodes. More preferably, hemipod pests such as cotton aphids, tobacco whiteflies and leafhoppers, thrips pests such as the southern thrips, lepidopterous insects such as the scallops, koga and chahamaki; Examples include, but are not limited to, sex nematodes.

When the microbial metabolite represented by the formula (1) is used as an insecticide, it may be used as it is, but a suitable solid carrier, liquid carrier, gaseous carrier, etc., a surfactant, a dispersant, and other formulation aids A preparation may be prepared by mixing with an agent and the like. As the preparation, preferably, emulsion, EW agent, liquid agent, suspension agent, wettable powder, granular wettable powder, powder, DL powder, powder, granule, tablet, oil, aerosol, flowable, dry flowable Agents, microcapsules, and the like. It can be used as a dosage form arbitrarily selected as these preparations. The carrier in the present invention refers to a solid carrier, a liquid carrier, a gaseous carrier and the like.

Examples of the solid carrier include talc, bennite, clay, kaolin, diatomaceous earth, vermiculite, white carbon, calcium carbonate, acid clay, silica sand, silica, zeolite, perlite, attapulgite, pumice, ammonium sulfate, sodium sulfate, urea, and the like. It is done.

Examples of the liquid carrier include alcohols such as methanol, ethanol, n-hexanol, ethylene glycol, and propylene glycol; ketones such as acetone, methyl ethyl ketone, and cyclohexanone; and aliphatic hydrocarbons such as n-hexane, kerosene, and kerosene. , Aromatic hydrocarbons such as toluene, xylene and methylnaphthalene, ethers such as diethyl ether, dioxane and tetrahydrofuran, esters such as ethyl acetate, nitriles such as acetonitrile and isobutyronitrile, dimethylformamide, dimethylacetamide and the like Acid amides, vegetable oils such as soybean oil and cottonseed oil, dimethyl sulfoxide, water and the like.

Examples of the gaseous carrier include LPG, air, nitrogen, carbon dioxide gas, dimethyl ether and the like.

Examples of the surfactant and the dispersant include alkyl sulfates, alkyl (aryl) sulfonates, polyoxyalkylene alkyl (aryl) ethers, polyhydric alcohol esters, lignin sulfonates, and alkyl sulfosuccinic acids. Salt, formalin condensate of alkyl naphthalene sulfonate, polycarboxylate, POE polystyryl phenyl ether sulfate and phosphate, POE / POP block polymer, and the like.

Furthermore, examples of the adjuvant for preparation include carboxymethyl cellulose, hydroxypropyl cellulose, polyvinyl alcohol, xanthan gum, pregelatinized starch, gum arabic, polyethylene glycol, liquid paraffin, calcium stearate, and antifoaming agent, preservative and the like. It is done.

The above-mentioned various carriers, surfactants, dispersants, and formulation adjuvants can be used alone or in combination as necessary.

The content of the microbial metabolite represented by formula (1), which is an active ingredient in the preparation, is not particularly limited, but is preferably 0.1 to 75% by weight for the emulsion and 0.03 to 50% for the powder. %, 0.1 to 90% by weight for wettable powders and 0.05 to 10% by weight for granules.

The pesticides according to the present invention can be used as they are or after dilution, but other pesticides, fungicides, insecticides for the purpose of controlling pests to be controlled, the expansion of the control period, or for the purpose of reducing the dose. It can be mixed or used in combination with mites, herbicides, plant growth regulators, fertilizers and the like.

Examples of drugs that can be mixed or used in combination include, for example, the Pesticide Manual (issued by The 16th Edition The British Crop Protection Council) and Shibuya Index (SHIBUYA INDEX 16th Edition, 2012, published by SHIBUYA INDEX RESARCH GROUP Y; 17) Edition, 2014, published by SHIBUYA INDEX RESEARCH GROUP) and Irac Mode of Action Classification (Mode of Action Classification Version 7.3, published by IRAC), Mode of Action of Fungiside e 2015 edition of the Annual Book, include those described in FRAC issue).

More specifically, the pesticides are, for example, alaniccarb, aldicarb, bendiocarb, bendiocarb, benfurcarb, butocalboxix, butoxycarbbox, carbaryl, carbofuran, carbosulfan, ethiofencarb, fenobucarb, formethanate, formethanate (Methiocarb), methomyl (methamil), thiomil (trimical) Carbamate compounds such as xylylcarb, metolcarb, phenothiocarb, phenoxycarb, acephate, azamethiphos, azinethiphos-thiethyl-phosphine zynphos-methyl, ethylthiomethone, chlorethoxyphos, chlorous fos, chlorpyfos, chlormefos, chlormefos methyl, coumaphos, cyanophos, demethon-S-methyl, diazinon, dichlorvos, dicrotophos, dimethoe Dimethoate, Dimethylvinphos, EPN, Ethion, Etoprophos, Famfur, Fenamifos, Fentithione, Fenthito, Fenthito, Fenthito Heptenophos, imicyafos, isofenphos, isopropyl = O- (methoxyaminebothiophosphoryl) salicylate [isopropyl O- (methoxyaminothiophosphoryl), isoxathionate, Malathion, mecarbam, methamidophos, methidathion, mevinphos, monocrotophos, naledo, oxymethione, methodithone (Parathion), parathion-methyl, PAP, forte, fosarone, phosmet, phosphamidon, phoxim, pyrimiphos-methyl (pirimimiphos) , Profenofos, Propetanfos, Prothiofos, Pyraclofos, Pyridaphenthion, Quinalphos, Sulfotep, Sulfotep , Tetrachlorvinphos, thiomethon, triazophos, trichlorfon, bamithiothion, chlorpyrifos-methyl ), Disulfoton, sulprofos, flupyrazofos, phentoate, phonofos, organophosphate compounds such as tribufos, endosulfan (endosulfan) Organochlorine compounds such as alpha-endosulfan, gamma-HCH (gamma-HCH), dicofol, chlordane, dieldrin, methoxychlor, acetoprole, fipronil ), Ethiprole (ethipr) le), pyrafluprole, pyriprole, phenylpyrazole compounds such as flufiprole, metadiamide compounds such as brofuranilide, axoxaner, ruralaner, lu Isoxazoline-based compounds such as saroraner, acrinathrin, allethrin, d-cis-trans allethrin, d-trans allethrin, bifenthrin, kappa birin-kappa birin -Cyclopentenyl (bioalle) thrin S-cyclopentenyl), violesmethrin, cycloprotorin, cyfluthrin, beta-cyfluthrin, halothrin (cyhalothrin) gamma-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, theta-cypermethrin-theta-cypermethrin-theta-cypermethrin-thetamethrin ypermethrin, cyphenothrin, deltamethrin, empentrin, esfenvalerate, etofenprox, fenpropravenate, fenpropravenate fluthytrinate, flumethrin, tau-fulvalinate, halfenprox, imiprothrin, kadesthrin, permethrin, permethrin enothrin, praretrin, pyrethrin, resmethrin, silafluofen, tefluthrin, kappa-tefluthrin, kappa-tefluthrin, kappa-tefluthrin, kappa-tefluthrin, kappa-tefluthrin ), Transfluthrin, methoxadiazone, methfluthrin, profluthrin, pyrethrum, teraretrin, mon Lumorosrin, heptafluthrin, meperfluthrin, tetramethylfluthrin, dimethylfluthrin, protoflubutrin Neonicotinoid compounds, such as (chlothidian), dinotefuran, imidacloprid, nitenpyram, thiacloprid, thiamethoxam, sulfoxa Sulfoxamine-based compounds such as floroxaflor, butenolide-based compounds such as flupyradidifurone, triflumezopyrim, meso-ionic compounds such as dichloromezothiaz, spiro-nezotium (spiro-nezotiz) spinosyn compounds such as spinetoram, abamectin, ivermectin, emamectin benzoate, macroremine compounds such as milbemectin, lepimectin, and hydropylene compounds such as lepictin. Kinopre Juvenile hormone-like compounds such as quinoprene, diophenolan and methoprene, 4-phenoxyphenoxy compounds such as pyriproxyfen, pyridine azomethine compounds such as pymetrozine Pyridinecarboxamide compounds such as flonicamid, oxazole compounds such as etoxazole, B.I. t. subsp. israelensis, B.I. t. subsp. aizawai, B.I. t. subsp. Kurstaki, B.I. t. subsp. Bacillus thuringiensis and Bacillus sphaericus agents such as tenebrionis and insecticidal proteins produced by them, insecticidal proteins produced by the above-mentioned Bt crops, thiourea compounds such as diafenthiuron, azocyclotin Organic metal compounds such as cyhexatin, fenbutatin oxide, sulfite ester-diphenyl ether compounds such as propargite, diphenylsulfone compounds such as tetradiphon, chlorfenapyr ), Tralopyril (tral) a pyrrole compound such as pyril, a dinitro compound such as DNOC, a benultap, a cartap, a thiocyclam, a thiosultap, and a nereistapine such as thiosultap sodium. Analogues, bistrifluron, chlorfluazuron, diflubenzuron, flucycloxuron, flufenoxuron, hexafluururon, hexflufluron Novaluro ), Noviflumuron (Noviflumuron), teflubenzuron (Teflubenzuron), triflumuron (Triflumuron), benzoyl urea compounds such as bistrifluron (Bistrifluron) thiadiazine compounds such as buprofezin (buprofezin). Triazole compounds such as cyromazine, chromafenozide, halofenozide, methoxyphenozide, amid hydrazine compounds such as tebufenozide, tehydrenodine Amidinohydrazone compounds such as methylmethylnon, naphthoquinone compounds such as acequinocyl, fluacrylpyrim, pyriminostrobin, flufenoxystrobin such as flufenoxystrobin Lovirlin compounds, quinazoline compounds such as fenazaquin, phenoxypyrazole compounds such as fenpyroximate, phenoxyethylamine compounds such as pyrimidifene, pyridazinone compounds such as pyridaben. Pyrazole carboxamide compounds such as tebufenpyrad, tolfenpyrad, pyflubumide, hydrazine carboxamide compounds such as metaflumizone, spirodiclofenet (spirodiclofenet) ), Tetronic and tetramic acid compounds such as spiromesifene, beta-ketonitrile compounds such as cyflumetofen, cienopyrafen, and phthalamide compounds such as flubendiamide , Anthranilic acid amide compounds such as chlorantraniliprole, cyantraniliprole, cyclaniliprole, tetraniliprole, quinothione methionate Compound, hexythiazox ( a thiazolidinone compound such as hexythiazox, a hydrazine compound such as bifenazate, a pyridinamine compound such as flufenerim, an aminoquinazoline compound such as pylofluquinazoin, a flotoquinoin such as lotokin. 6-phenoxyquinoline compounds, pyridinylethylbenzamide compounds such as fluopyram, 8-chloro-N-((2-chloro-5-methoxyphenyl) sulfonyl) -6- (trifluoromethyl) imidazo It may be a sulfonamide compound such as [1,2-alpyridine-2-carboxamide], amidoflumet.

Other insecticides include nicotine, chloropicrin, sulfuryl fluoride, crylotie, clofentezine, diflovidazine, diflovidazine Xoxacarb (indoxacarb), piperonyl butoxide, chlordiform, pyridalyl, azadirachtin, benzoxifide, benzoximate Thioxazafen, fluensulfone, benclothiaz, carzole, insecticidal soap, dimethylhypo, nithiazine, borate meta, salt aldehyde Ryanodine, sulfuramid, (E) -N- (1-((6-chloropyridin-3-yl) methyl) pyridine-2 (1H) -ylidene) -2,2,2-trifluor And compounds such as loacetamide, 5- (trifluoromethyl) -2- (4- (4- (trifluoromethyl) phenoxy) piperidin-1-yl) pyridine. Furthermore, the insecticide according to the present invention can be used in combination or in combination with microbial pesticides such as entomopathogenic bacteria, entomopathogenic viruses and entomopathogenic fungi.

The fungicides used are, for example, metalaxyl, metalaxyl-M, oxadixyl, ofurase, benalaxyl, benalaxyl-M, Phylamide-type compounds such as offuras, furalaxyl, cyprofuram, buplymate, dimethylimole, hydroxypyrimidine-type compounds such as ethilimol, Isoxazole (hydroxyisoxaz) le) isoxazole compounds, oxathiapiprolin piperidinyl thiazole isoxazoline compounds, isothiazolone compounds such as octylinone, carboxylic acids such as oxolinic acid Compounds such as benomyl, thiophanate-methyl, carbendazim, benzoids such as thiabendazole, thiabendazole, decarbcarb N-Feni Carbamate compounds, toluamide compounds such as zoxamide, ethylaminothiazole carboxamide compounds such as ethaboxam, phenylurea compounds such as pencyclon, and pyridinides such as flupicolide Pyrimidineamine compounds such as rumethylbenzamide compounds, diflumetorim, bupirimate, pyrazole carboxamide compounds such as tolfenpyrad, benodanil, flutolanil, protililil Benzanilide compounds such as Phenyloxoethylthiophenamide compounds such as isofetamid, pyridinylethylbenzamide compounds such as fluopyram, furan carboxamide compounds such as fenfuram, oxycarboxin, carboxy Oxatiin carboxamide compounds such as carboxin, thiazole carboxamide compounds such as thifluzamide, floxapyroxad, furametopyr, penflufen, entiopyradop Diflupill (bizovindiflupyr), bi Pyrazole-4-carboxamide compounds such as bixafen, isopyrazam, sedaxane, pyridinecarboxamide compounds such as boscalid, azoxystrobin, komethoxystrobin ( cosmetrostrobin, cresoxym-methyl, trifloxystrobin, picoxystrobin, pyracrostrobin, dimoxystrobin, dimoxystrobin, dimoxystrobin ory astrobin, fluoxastrobine, pyraoxystrobin, pyramethostrobin, flufenoxystrobin, phenaminestrobin (en), enoxastrobin, enoxastrobin Strobilurin compounds such as stroxybin, mandestrobin, triclopyricarb, oxazolidinedione compounds such as famoxadone, imidazolinones such as fenamidone Benzylcarbamate compounds such as triclopyricarab, pyribencarb, cyanoimidazole compounds such as cyazofamid, sulfamoyltriazole compounds such as amisulbrom, binapacyl (bin) acryl Dinitrophenyl croton compounds such as meptyldinocarb, dinocap, 2,6-dinitroaniline compounds such as fluazinam, and pyrimidinone hydrazone compounds such as ferimzone. , Acetate-fentin, fentin chloride (fe organic / inorganic metal compounds such as ntin chloride, fentin hydroxide, triphenyltin hydroxide, triphenyltin acetate, oxine copper, silthiofam ), Thiophene carboxamide compounds, triazolopyrimidine amine compounds such as amethoctradin, mepanipyrim, nitrapyrin, pyrimethanil, and cyprodinyl (cyprodinol) , Blasticidin S (blasticidi Enopyranuronic acid antibiotics such as -S), kasugamycin, hexopyranosyl antibiotics such as kasugamycin hydrochloride hydrate, glucopyranosyl antibiotics such as streptomycin, Tetracycline antibiotics such as quinoxyphen, allyloxyquinoline compounds such as quinoxyfen, quinazoline compounds such as proquinazid, fludioxonil, cyanopyrrole compounds such as fenpiclonil, fluoroimide ( fluor imid, procymidone, iprodione, dicarboximide-based compounds such as vinclozolin, edifenphos, iprobenfos, phospholothios-based compounds such as pyrazothios Dithiolane compounds such as isoprothiolane, propamocarb, propyl carbamate compounds such as propamocarb hydrochloride, strains of Bacillus subtilis (QST 713MB, BST Bactericidal proteins produced, bactericidal proteins produced by the above Bt crop, terpene hydrocarbons and terpene alcohols such as an extract of Goseika Yupte, piperazine compounds such as triforine, Pyridine compounds such as pyrifenox, pyrisoxazole, pyrimidine compounds such as fenarimol, nuarimol, azaconazole, bromuconazole, bromuconazole , Diniconazole-M, epoxiconazole, fluquinconazo Fluquinconazole, oxpoconazole, pefurazoate, difenoconazole, fenbuconazole, imibenconazole. Tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole, imazalil, bitterol, bitterol Lumizole (trifluolazole), etaconazole (epaconazole), propiconazole (propiconazole), penconazole (fluconazole), flutriazole (b), crotriazole (pro), ), Cyproconazole, tebuconazole, hexaconazole, prochloraz, simoneazole (simeconazole), aldimorph (al) imorph, dodemorph, dodemorph acetate, tridemorph, fenpropimorph, dimethorph, phulmorph, pylmorph Piperalin, piperidine compounds such as fenpropidin, spiroketal amine compounds such as spiroxamine, hydroxyanilide compounds such as fenhexamide, fenpyrazamine, and the like. Aminopyrazolinone compounds Ferbam, metam, metasulfocarb, methiram, thiram, manzeb, manneb, zineb, ziram, polyram polycarbamate, propineb, thiuram, thiocarbamate and dithiocarbamate compounds such as pyributicalcarb, glucopyranosyl antibiotics such as validamycin, myrdiomycin Nucleoside antibiotics such as Bench Avaricarb (benth avalamides such as valivalamide carbamates such as iavalicarb, benthiavalcarb-isopropyl, valifenalate, iprovalivalb, and amanamides such as mandipropamide. ), An isobenzofuranone compound such as pyrroloquinone, a pyrroloquinolinone compound such as pyroquilone, a triazolobenzothiazole compound such as tricyclazole, a cyclopropanecarboxamide compound such as carpropamide, and diclocimet ( dicyclome ), Carboxamide compounds such as phenoxanil, propionamide compounds such as phenoxanil, benzothiadiazole compounds such as acibenzolar-S-methyl, and benzoisothiazoles such as probenazole. Compounds, thiadiazole carboxamide compounds such as thiadinil, isothiazole carboxamide compounds such as isotianil, cyanoacetamide = oxime compounds such as cymoxanil, ethyl such as fosetyl Phosphonate compounds, phthalamic acid compounds such as techlophthalam, Benzotriazine compounds such as triazoxide, benzenesulfonic acid compounds such as flusulfamide, pyridazinone compounds such as dicromezine, phenylacetamide compounds such as cyflufenamide, metolaphenone benzophenone compounds such as metfafenopne, benzoylpyridine compounds such as pyriophenone, cyanomethylene thiazolidine compounds such as flutianil, 4-quinolyl acetate compounds such as tebufloquin, fosetyl aluminum (Fosetyl-aluminium), Torr Organophosphorous compounds such as chlorfos-methyl, 1,2,4-thiadiazole compounds such as echromazole, trifluoroethyl carbamate compounds such as tolprocarb, Bordeaux ) Mixture, copper acetate, basic copper sulfate, copper oxychloride, copper hydroxide, oxyquinoline copper (oxine-copper) Copper compounds, inorganic compounds such as copper and sulfur, captan, captafol, holpet and so on A halogenothioalkyl-based compound, anilazine, chlorothalonil, dichlorophen, pentachlorophenol and salts thereof, an organic chlorine such as hexachlorobenzene, a quintozene , Iminoctadine triacetate salt, iminoctadine albesylate, guanidine, dodin, dodine free base, guazatine acetate, guazatine acetate, guazatine salt salt), guanidine compounds such as albesylate, anthraquinone compounds such as dithianon, quinoxaline compounds such as quinomethionate, maleimide compounds such as fluorimide, tolylfluanid There may be a sulfenic acid-based compound such as (tolylfluorid) and diclofluuride, a dinitrophenol-based compound such as dinobuton, and a cyclic dithiocarbamate-based compound such as dazomet. In addition, as other fungicides, dipymethitrone, pyraziflumide, picarbutrazox, technazen, nitralt-isopropyl, dicyclometol, dicyclometyl ), Prohexadione-calcium, bronopol, diphenylamine, flumetover, bentoxazine, biphenyl, chloroneob, chloroneodo Rub (iodcarb), prothiocarb, N- (1- (2,4-dichlorophenyl) -1-methoxypropan-2-yl) -2-iodobenzamide, 3- (5-fluoro-3,3, 4,4-tetramethyl-3,4-dihydroisoquinolin-1-yl) quinoline, 3- (4,4-difluoro-3,3-dimethyl-3,4-dihydroisoquinolin-1-yl) quinoline, 3- (4,4,5-trifluoro-3,3-dimethyl-3,4-dihydroisoquinolin-1-yl) quinoline and the like.

<Example 1> Cotton aphid test A square leaf piece having a side of 3.5 cm was cut out from a cucumber leaf. It was placed on absorbent cotton (7 cm in diameter) moistened with water to create a leaf disk. Two adult aphids were released here. On the next day, it was confirmed that 5 to 15 instar larvae were infested, and adults were removed. 100 μL of a sample aqueous solution prepared by adjusting the microorganism metabolite represented by the formula (1) to a predetermined concentration was sprayed on a leaf disk using an airbrush (Tamiya SPRAY-WORK BASIC AIRBRUSH). After air drying, the leaf disc was transferred to a plastic cup, covered and left in a constant temperature room (25 ° C.). Five days after the release, the death rate was calculated according to the formula (I). Abnormal insects were included in dead insects.

Death rate (%) = {number of dead insects / (number of surviving insects + number of dead insects)} × 100 (1)

As a result of this test, the treatment with the sample aqueous solution having a concentration of 200 ppm showed a mortality rate of 100%.

<Example 2> Nami spider mite test A square leaf piece having a side of 2 cm was cut out from a kidney bean leaf. It was placed on absorbent cotton (5.5 cm in diameter) moistened with water to create a leaf disk. 100 μL of a sample aqueous solution prepared by adjusting the microorganism metabolite represented by the formula (1) to a predetermined concentration was sprayed on a leaf disk using an airbrush (Tamiya SPRAY-WORK BASIC AIRBRUSH). After air-drying, the leaf disc was transferred to a plastic cup, and 3 female spider mites were released here, covered, and left in a constant temperature room (25 ° C.). Three days after the release, the next-generation density suppression rate was calculated according to the formula (II).

Next-generation density suppression rate (%) = {1− (number of next-generation larvae in treated group / number of next-generation larvae in untreated group)} × 100... Formula (II)

As a result of this test, the treatment of the sample aqueous solution having a concentration of 400 ppm showed a next-generation density suppression rate of 80% or more.

<Example 3> Hasmonyoto test 100 microliters of sample aqueous solution which prepared the microorganism metabolite represented by Formula (1) to predetermined | prescribed density | concentration was dripped with respect to 300 mg of artificial feed (Insector LFS; Nippon Agricultural Industry Co., Ltd.). After air-drying the surface of the artificial feed so that it was not too dry, 3 2nd instar larvae were released, covered and left in a constant temperature room (25 ° C.). Three days after release, the death rate was calculated according to the formula (III). Abnormal insects were included in dead insects.

Mortality rate (%) = {number of dead insects / (number of surviving insects + number of dead insects)} × 100... Formula (III)

As a result of this test, a 100% death rate was exhibited by spraying a sample aqueous solution having a concentration of 400 ppm.

<Example 4> A sample aqueous solution in which the microbial metabolite represented by the formula (1) is adjusted to a concentration twice as high as a predetermined test concentration with respect to the suspension water of the second stage larvae of the sweet potato nematode isolated from the test eggplant. Was added to the nematode suspension to treat the drug solution. Six days after the treatment, the corrected abnormal immobility was calculated according to the formula (IV).

Corrected abnormal immobility rate (%) = {(number of abnormal insects + number of immobile insects) / (number of normal insects + number of abnormal insects + number of immobile insects)-(number of abnormal untreated insects + number of untreated immobile insects) / (no processing Number of normal insects + number of untreated abnormal insects + number of untreated immobile insects)} / {number of normal untreated insects / (number of normal untreated insects + number of untreated abnormal insects + number of untreated immobile insects)} × 100... Formula (IV)

As a result of this test, a corrected abnormal immobility rate of 50% or more was shown in the treatment of the sample aqueous solution having a concentration of 400 ppm.

<Example 5> A circular leaf piece having a diameter of 3 cm was cut out from a cabbage test cabbage leaf, and the microbial metabolite represented by the formula (1) was immersed in an aqueous sample solution prepared to a predetermined concentration by adding 1500 times of Grameen S. After air-drying, this was put in a plastic cup, and three third-instar larvae were released. Thereafter, the plastic cup was covered and left in a constant temperature room (25 ° C.). The insects were observed for life and death 3 days after the release, and the death rate was calculated according to the formula (V). Abnormal insects were included in dead insects.

Death rate (%) = {Number of deaths / (Number of surviving insects + Number of dead insects)} × 100 Formula (V)

As a result of this test, a 100% death rate was exhibited by spraying a sample aqueous solution having a concentration of 400 ppm.

<Example 6> A white planthopper test One rice seedling was taken, and Grameen S1500 times was applied, and the microorganism metabolite represented by the formula (1) was immersed in 200 μL of a sample aqueous solution prepared to a predetermined concentration and air-dried. . After air drying, it was held in a glass cylinder (inner diameter 2.8 cm × 15 cm) using urethane and placed in a 100 ml conical beaker containing 30 ml of water. To this, 10 3rd-instar larvae were released. The lid was covered with medicine wrapping paper and left in a constant temperature room (25 ° C.). The mortality of the larvae was observed 3 to 5 days after release, and the mortality rate was calculated according to the formula (VI). Abnormal insects were included in dead insects.

Mortality (%) = {number of deaths / (number of surviving insects + number of dead insects)} × 100... Formula (VI)

As a result of this test, the treatment with the sample aqueous solution having a concentration of 400 ppm showed a mortality rate of 100%.

Claims (1)

An insecticide containing the microbial metabolite represented by formula (1) or a salt thereof.