JP2016130229A - Method for producing amino acid dissolved product and amino acid dissolved product produced by the production method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for producing an amino acid dissolved product that allows valine, leucine and isoleucine to be dissolved with excellent stability, and an amino acid dissolved product produced by the production method.SOLUTION: A method for producing an amino acid dissolved product is characterized in that (A) amino acids are valine, leucine and isoleucine, and (A) the amino acids are blended with (B) N-acetylglucosamine for dissolution. There is also provided an amino acid dissolved product produced by the production method. Preferably, (A) the amino acids are valine 0.1-2.0 mass%, leucine 0.1-4.0 mass% and isoleucine 0.1-2.0 mass%, and (B) N-acetylglucosamine is 0.1-4.0 mass%.SELECTED DRAWING: None

Description

  The present invention relates to a method for producing an amino acid lysate and an amino acid lysate produced by the production method, and more particularly, to a method for producing an amino acid lysate capable of dissolving valine, leucine and isoleucine in a stable state, and the production method. It relates to the amino acid lysate produced.

  Valine, leucine and isoleucine are one of the essential amino acids called BCAA (Branched Chain Amino Acids) and have been conventionally incorporated into foods and the like for the purpose of sports anemia and recovery from fatigue. In addition, since it is a component constituting the protein of human epidermis, it has also been incorporated into external preparations such as cosmetics and pharmaceuticals.

  For example, for food use, Patent Document 1 discloses a solid solution composed of three amino acids of valine, isoleucine and leucine, and Patent Document 2 discloses L-arginine, L-glutamine, L-valine, L-isoleucine and 5 types of amino acids of L-leucine are contained in a ratio of 10 to 40 parts by weight, 10 to 40 parts by weight, 5 to 20 parts by weight, 8 to 30 parts by weight and 10 to 35 parts by weight, respectively. An oral amino acid composition for improving skin condition is disclosed.

  As the use of the external preparation, Patent Document 3 includes glycine, alanine, valine, leucine, isoleucine, serine, threonine, aspartic acid, glutamic acid, methionine, lysine, oxylysine, arginine, histidine, phenylalanine, tyrosine, proline and oxy. A collagen production promoter comprising an amino acid mixture composed of proline is disclosed, and Patent Document 4 discloses that component (A) an amino acid having a positive Hydropathy Index and (B) one type selected from menthol, menthol derivatives, or camphor A moisturizing agent containing at least two kinds as active ingredients is disclosed.

  On the other hand, N-acetylglucosamine is a kind of amino sugar and is an important carbohydrate component that is also present in the human body, so it has traditionally been incorporated into skin preparations such as foods, cosmetics, and pharmaceuticals. It had been.

  For example, Patent Document 5 contains at least one amino acid selected from valine, leucine or isoleucine together with α-lipoic acid and coenzyme Q10, and further contains vitamin A, vitamin B, vitamin C and vitamin E. , Vitamin K, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid, hydroxylysine, arginine, ornithine, histidine, Taurine, collagen, glucosamine, acetylglucosamine, hyaluronic acid, isoflavone, polyphenol, biotin, whey peptide, soybean peptide, royal jelly, phytonadione, An internal use composition containing at least one selected from naquinone, menadione, menadiol, ferulic acid, γ-oryzanol, orotic acid, rutin, eriocitrin, hesperidin or a salt thereof is disclosed. A) A hair treatment composition containing glucosamines, wherein the (A) glucosamine is at least one of glucosamine and acetylglucosamine, and further contains (B) acidic amino acids and (C) basic amino acids. A hair treatment composition characterized by the above is disclosed.

International Publication No. 2010/050168 Pamphlet JP 2005-247738 A JP 2007-51087 A JP2013-227286A JP 2006-151909 A JP 2010-285390 A

  However, as described in Patent Document 1, the solubility of valine, isoleucine, and leucine at 20 ° C. is 5.75 g, 4.02 g, and 2.38 g, which are soluble in water. Since the carbon chain is hydrophobic, a phenomenon such as floating on the water surface occurs, and the stability of the amino acid solution containing valine, leucine and isoleucine was extremely poor. This problem cannot be solved by the techniques described in Patent Documents 1 to 4, and improvements have been desired.

  In addition, although the techniques described in Patent Documents 5 and 6 are described in which N-acetylglucosamine is blended, the stability of the amino acid lysate blended with valine, leucine and isoleucine is not taken into consideration. This technique also did not mention the solubility effect of valine, leucine and isoleucine by adding N-acetylglucosamine.

  Accordingly, an object of the present invention is to solve the above-mentioned problems of the prior art, a method for producing an amino acid solution capable of dissolving valine, leucine and isoleucine in a good stability state, and an amino acid solution produced by the production method Is to provide.

  As a result of intensive studies to solve the above problems, the present inventor has found that the object can be achieved by blending a specific amino acid and N-acetylglucosamine and dissolving them in water. It came to be completed.

That is, the method for producing the amino acid lysate of the present invention comprises:
(A) the amino acids are valine, leucine and isoleucine,
The (A) amino acid and (B) N-acetylglucosamine are mixed and dissolved.

  Moreover, the manufacturing method of the amino acid melt | dissolution of this invention WHEREIN: The said (A) amino acid is valine 0.1-2.0 mass%, leucine 0.1-4.0 mass%, and isoleucine 0.1-2.0. It is preferable that the (B) N-acetylglucosamine is 0.1 to 4.0% by mass.

  Furthermore, in the method for producing an amino acid lysate of the present invention, it is preferable to mix (C) anserine and dissolve, and (D) to mix and dissolve an acrylic acid amide water-soluble thickener, E) It is preferable to mix and dissolve at least one selected from the group consisting of PEG-20 sesquistearate and PEG-20 dioleate.

  Furthermore, in the method for producing an amino acid solution of the present invention, the amino acid solution is preferably a food or drink, the amino acid solution is preferably a skin external preparation, and the amino acid solution is a hair restorer. It is preferable.

  The amino acid lysate of the present invention is characterized by being produced by the method for producing an amino acid lysate.

  ADVANTAGE OF THE INVENTION According to this invention, the manufacturing method of the amino acid solution which can melt | dissolve a valine, a leucine, and isoleucine in a favorable state can be provided, and the amino acid solution manufactured by this manufacturing method can be provided.

Hereinafter, the method for producing an amino acid solution of the present invention and the amino acid solution produced by the production method will be specifically described.
The method for producing an amino acid lysate according to the present invention is characterized in that (A) the amino acid is valine, leucine and isoleucine, and the (A) amino acid and (B) N-acetylglucosamine are mixed and dissolved. is there. The amino acid lysate of the present invention is characterized by being produced by the method for producing such an amino acid lysate. The valine, the leucine, and the isoleucine were mixed with the (B) N-acetylglucosamine and dissolved in water, and this was caused by forced mixing with a thickening agent or the like with high viscosity. A phenomenon such as valine, leucine and isoleucine floating on the water surface in the lysate can be prevented, and an amino acid lysate having good dissolution stability of valine, leucine and isoleucine can be provided. In addition, (B) by adding N-acetylglucosamine, the valine, the leucine and the isoleucine can be dissolved well in the amino acid solution, and the permeability into the body, skin and hair is improved. By ingesting or applying an amino acid solution, fatigue of muscles and the like can be prevented. Specifically, the muscle fatigue of the foot during standing work can be reduced by oral intake of the amino acid solution or application to thighs or calves. In the present invention, the “compounding” may be included in the formulation, and the addition method and procedure are not limited.

  In the present invention, the valine, the leucine, and the isoleucine are not limited as long as they can be used for ordinary foods and drinks, external preparations, and the like. For example, L-valine (trade name) manufactured by Ajinomoto Healthy Supply Co., Ltd. , L-leucine (trade name), L-isoleucine (trade name) and the like.

  In the present invention, the (B) N-acetylglucosamine is not limited as long as it can be used for ordinary foods and drinks, external preparations, and the like. For example, the shell of shellfish such as crabs and shrimps is derived. The natural polysaccharide chitin is obtained by a known method such as hydrolysis with an acid and an enzyme, and the production method is not particularly limited. Specific examples include Marine Sweet F (trade name) and Marine Sweet YSK (trade name) manufactured by Yaizu Suisan Chemical Co., Ltd.

  Furthermore, the method for producing the amino acid lysate of the present invention comprises the valine 0.1 to 2.0% by mass, the leucine 0.1 to 4.0% by mass and the isoleucine 0.1 to 2.0% by mass, The (B) N-acetylglucosamine 0.1 to 4.0% by mass is preferably blended, and the valine 0.5 to 1.0% by mass, the leucine 0.5 to 2.0% by mass, and More preferably, 0.5 to 1.0 mass% of the isoleucine and 0.5 to 2.0 mass% of (B) N-acetylglucosamine are blended. By setting the blending amount in such a range, the dissolution stability of the valine, the leucine and the isoleucine in the amino acid solution can be improved, and the effect of preventing the fatigue of muscles and the like due to the intake or application of the amino acid solution can be further increased. be able to.

  In the method for producing an amino acid lysate of the present invention, the blending ratio of the valine, the leucine and the isoleucine and the (B) N-acetylglucosamine is preferably 1: 2: 1: 2. More preferably, 0.5% by mass of the valine, 1.0% by mass of the leucine, 0.5% by mass of the isoleucine, and 1.0% by mass of the (B) N-acetylglucosamine are blended. By setting leucine to a blending ratio of about twice, the effect of preventing the fatigue of muscles and the like caused by ingestion or application of an amino acid solution can be further enhanced.

  Furthermore, in the method for producing an amino acid lysate of the present invention, it is preferable to mix and dissolve an imidazole dipeptide compound. Examples of such imidazole dipeptide compounds include anserine (β-alanyl-1-methylhistidine), Carnosine (β-alanylhistidine), valenine (β-alanyl-3-methylhistidine) and the like can be mentioned. The imidazole dipeptide compounds are contained in fish meat, poultry meat, livestock meat, etc. For example, anserine is contained in meat such as skipjack, tuna, cow, chicken, etc., and carnosine is contained in pork. Valenin is abundant in whale meat (eg, baleen whales). As the imidazole dipeptide compounds, those obtained by purifying extracts extracted therefrom by a method such as water extraction, hot water extraction, alcohol extraction, supercritical extraction or the like can be used.

  Furthermore, in the method for producing an amino acid lysate of the present invention, it is preferable to dissolve (C) anserine (β-alanyl-1-methylhistidine), particularly 0.1 to 3.0% by mass. It is preferable to do. The (C) anserine is not limited as long as it can be used for ordinary foods and drinks, external preparations, etc. For example, anserine can be obtained as follows. First, an extract was prepared from meat such as skipjack, tuna, cow, chicken, etc. according to a conventional method, and water was added as appropriate to adjust the Brix (Bx.) Value (refractive sugar content reading) of the extract to 1 to 10%. Then, high molecular protein is removed using an ultrafiltration membrane (fraction molecular weight 5,000-50,000), and a low molecular weight peptide fraction is collect | recovered. Then, according to the method of the literature (Suyama et al: Bull. Japan. Soc. Scient. Fish., 33, 141-146, 1967), ion exchange chromatography using a strongly acidic resin was performed by appropriately concentrating the low molecular peptide fraction. And collect the eluate. The eluate can be obtained by desalting, adjusting the pH, and drying by freeze drying or the like.

  By dissolving the imidazole dipeptide compound, particularly (C) anserine, it is possible to further enhance the effect of preventing the fatigue of muscles and the like due to the intake or application of the amino acid solution.

  Moreover, it is preferable that the manufacturing method of the amino acid melt | dissolution of this invention mix | blends and dissolves (D) acrylic-amide-type water-soluble thickener. The (D) acrylic acid amide-based water-soluble thickener is not limited as long as it can be used for ordinary foods and beverages, external preparations, and the like. For example, (hydroxyethyl acrylate / acryloyldimethyltaurine Na) copolymer, (Acrylic acid Na / acryloyl dimethyl taurine Na) copolymer, (acryloyl dimethyl tauric ammonium ammonium / VP) copolymer, (acryloyl dimethyl tauric ammonium ammonium behenes-25) crosspolymer, and the like. Among them, (hydroxyethyl acrylate / An acryloyldimethyltaurine Na) copolymer and an (acrylic acid Na / acryloyldimethyltaurine Na) copolymer are particularly preferred, and one or two or more kinds selected from the above-mentioned acrylic amide water-soluble thickeners are used. Door can be.

  Furthermore, as the (D) acrylic acid amide-based water-soluble thickener, specifically, as (Hydroxyethyl acrylate / acryloyldimethyltaurine Na) copolymer, SEPIPLUS S (trade name) manufactured by SEPPIC, SEPINOV EMT10 (trade name), SIMULGEL NS (trade name), and the like (Na / acrylic dimethyl taurine Na) copolymer may include SIMULGEL EG (trade name) manufactured by SEPPIC, Examples of the (acryloyldimethyltaurine ammonium / VP) copolymer include Aristoflex AVC (trade name) manufactured by Clariant Co., Ltd., and (acryloyldimethyltaurine ammonium methacrylate behenes-25) cross poly. Examples of the mer include Aristoflex HMB (trade name) manufactured by Clariant.

  By blending the (D) acrylic acid amide-based water-soluble thickener, the dissolution stability of the valine, the leucine and the isoleucine in the amino acid solution can be further improved, and the muscle produced by the application of the amino acid solution The effect of preventing fatigue such as this can be further enhanced.

  Furthermore, it is preferable that the manufacturing method of the amino acid melt | dissolution of this invention mix | blends and melt | dissolves 1 or more types chosen from the group which consists of (E) sesquistearic acid PEG-20 and dioleic acid PEG-20 methyl glucose. The (E) sesquistearic acid PEG-20 and dioleic acid PEG-20 methyl glucose are not limited as long as they can be used for ordinary foods and beverages, external preparations, etc., for example, Glucamate SSE manufactured by Nippon Lubrizol Corporation -20 (trade name), glucamate DOE-120 (trade name) and the like. Thereby, the dissolution stability of the valine, the leucine and the isoleucine in the amino acid solution can be further improved, and the effect of preventing fatigue of muscles and the like due to the application of the amino acid solution can be further enhanced.

  Moreover, in this invention, as water which melt | dissolves the said valine, the said leucine, the said isoleucine, and the said (B) N-acetylglucosamine in an amino acid melt, if it can be used for a normal food and drink, an external preparation, etc. Without being limited, purified water or the like can be used.

  Furthermore, in the method for producing an amino acid solution of the present invention, the amino acid solution is preferably a food or drink, the amino acid solution is preferably a skin external preparation, and the amino acid solution is a hair restorer. Is preferred. Here, the food and drink is a broad concept including health foods such as beverages, foods, and capsules, and may be anything that dissolves the valine, the leucine, the isoleucine, and the like in water. By taking it orally as a food or drink, fatigue such as muscles can be prevented. Further, the external preparation for skin may be any one that dissolves the valine, the leucine, the isoleucine, etc. in water, and can be used on the human body, hair, etc. Drugs can be targeted. Here, cosmetics and the like include basic cosmetics such as pack agents, makeup cosmetics, cosmetics for hair, bathing agents, and the like. By applying it to the skin as a skin external preparation or pack, fatigue of muscles and the like can be prevented, and by applying it to the hair as a hair restorer, a hair growth effect can be exhibited.

  Further, in the present invention, other components and the like can be appropriately added as long as the effects of the present invention are not impaired. Arbitrary ingredients other than the above can be blended in the qualitative and quantitative ranges, and ingredients usually blended in foods and drinks, external preparations, etc., for example, oily ingredients, moisturizers, antioxidants, preservatives, fragrances, Various vitamins, chelating agents, coloring agents, ultraviolet absorbers, medicinal ingredients, inorganic salts and the like can be blended.

  EXAMPLES Hereinafter, although this invention is demonstrated further in detail using an Example, this invention is not limited to these Examples. In addition, the numerical value in prescription shows the mass% or a mass part.

(Example 1, Comparative Example 1)
According to the formulation shown in Table 1 below, the skin external preparations of Example 1 and Comparative Example 1 were prepared. The obtained skin external preparations of Example 1 and Comparative Example 1 were subjected to the following stability test and fatigue recovery test, and are also shown in Table 1. In addition, the numerical value in prescription shows the mass%. In Table 1, * 1 is SEPIPULUS S (trade name) manufactured by SEPPIC.

(Stability test)
The test sample was stored at 40 ° C. for 1 month, and the presence or absence of precipitation or floating of valine, leucine and isoleucine was confirmed.

(Fatigue recovery test)
Targeting 28 healthy female volunteers, the sample of Example 1 was applied to the thigh and calf of the subject's right foot, the sample of Comparative Example 1 was applied to the thigh and calf of the subject's left foot, and standing for 8 hours. The fatigue feeling after the evaluation was evaluated according to the following evaluation criteria, and the total score of 28 people was determined.

(Fatigue evaluation criteria)
No fatigue 2 points Weak fatigue 1 point Strong fatigue 0 points

  From the results shown in Table 1, the skin external preparation of Example 1 had good stability and good fatigue recovery effect. On the other hand, the external preparation for skin of Comparative Example 1 was poor in stability and did not provide a sufficient fatigue recovery effect.

(Example 2, comparative example 2)
According to the formulation shown in Table 2 below, pack agents of Example 2 and Comparative Example 2 were prepared. The obtained pack agents of Example 2 and Comparative Example 2 were subjected to the above stability test and the following tension test, and are also shown in Table 2. In addition, the numerical value in prescription shows the mass%. In Table 2, * 2 is Tornale (trade name) manufactured by Hayashibara Co., Ltd.

(Stress test)
Targeting 28 healthy female volunteers, the sample of Example 2 is packed on the right half of the subject's face, the sample of Comparative Example 2 is packed on the left half of the subject's face, and the elasticity after 2 hours is shown below Evaluation was made according to the evaluation criteria, and a total score of 28 people was determined.

(Evaluation criteria for elasticity)
Sense of elasticity 2 points Weakness of elasticity 1 point No sensation 0 points

  From the results shown in Table 2, the pack agent of Example 2 had good stability and a good feeling of firmness. On the other hand, the pack agent of Comparative Example 2 was poor in stability and did not provide sufficient firmness.

(Example 3, Comparative Example 3)
According to the formulation shown in Table 3 below, hair restorers of Example 3 and Comparative Example 3 were prepared. The obtained hair growth agents of Example 3 and Comparative Example 3 were subjected to the above stability test and the following hair growth feeling test, and are also shown in Table 3. In addition, the numerical value in prescription shows the mass%. In Table 3, * 1 is SEPIPULUS S (trade name) manufactured by SEPPIC.

(Hair growth test)
Targeting 20 healthy male volunteers, the sample of Example 3 was applied to the right hair and head of the subject, and the sample of Comparative Example 3 was applied to the left hair and head of the subject. The hair growth feeling was evaluated according to the following evaluation criteria, and judged by the total score of 20 people.

(Evaluation criteria for hair growth feeling)
Hair growth effect 2 points Weak hair growth effect 1 point No hair growth effect 0 points

  From the results shown in Table 3, the hair restorer of Example 3 had good stability and good hair growth effect. On the other hand, the hair restorer of Comparative Example 3 was poor in stability and could not obtain a sufficient effect of hair growth.

(Example 4, comparative example 4)
According to the prescription of the following Table 4, the drinking water of Example 4 and Comparative Example 4 was produced. The drinking water obtained in Example 4 and Comparative Example 4 was subjected to the above stability test and the following intake fatigue recovery test, and are shown in Table 4 together. In addition, the numerical value in prescription shows a mass part.

(Intake fatigue recovery test)
For 28 healthy female volunteers, both when the drinking water of Example 4 was ingested and when the drinking water of Comparative Example 4 was ingested, the fatigue feeling after standing for 8 hours after ingestion Evaluation was carried out according to the following evaluation criteria, and a total score of 28 people was determined.

(Fatigue evaluation criteria)
No fatigue 2 points Weak fatigue 1 point Strong fatigue 0 points

  From the results in Table 4, the drinking water of Example 4 had good stability and good intake fatigue recovery effect. On the other hand, the drinking water of Comparative Example 4 was poor in stability and could not obtain a sufficient intake fatigue recovery effect.

(Example 5)
The skin external preparation of Example 5 was produced according to the prescription of Table 5 below. About the obtained skin external preparation of Example 5, said stability test and fatigue recovery test were done. In Table 5, * 1 is SEPIPULUS S (trade name) manufactured by SEPPIC.

  The skin external preparation of Example 5 had good stability and good fatigue recovery effect.

(Example 6)
A pack agent of Example 6 was produced according to the formulation shown in Table 6 below. With respect to the obtained pack agent of Example 6, the stability test and the firmness test were performed. In Table 6, * 2 is Tornale (trade name) manufactured by Hayashibara Co., Ltd.

  From the results shown in Table 6, the pack agent of Example 6 had good stability and a good feeling of elasticity.

(Example 7)
The hair restorer of Example 7 was produced according to the prescription in Table 7 below. The obtained hair growth agent of Example 7 was subjected to the above stability test and hair growth feeling test. In Table 7, * 1 is SEPIPULUS S (trade name) manufactured by SEPPIC.

  The hair restorer of Example 7 had good stability and a good effect on hair growth feeling.

(Example 8)
The drinking water of Example 8 was produced according to the prescription in Table 8 below. About the obtained drinking water of Example 8, said stability test and said intake fatigue recovery test were done.

  The drinking water of Example 8 had good stability and good intake fatigue recovery effect.

Example 9
The lotion of Example 9 was produced according to the prescription in Table 9 below. The obtained lotion of Example 9 was subjected to the above stability test and fatigue recovery test.

  The lotion of Example 9 had good stability and good fatigue recovery effect.

(Example 10)
The lotion of Example 10 was produced according to the prescription in Table 10 below. The obtained lotion of Example 10 was subjected to the above stability test and fatigue recovery test.

  The lotion of Example 10 had good stability and good fatigue recovery effect.

(Example 11)
A cream of Example 11 was prepared according to the formulation shown in Table 11 below. The obtained cream of Example 11 was subjected to the above stability test and fatigue recovery test.

  The cream of Example 11 had good stability and good fatigue recovery effect.

(Example 12)
A cream of Example 12 was prepared according to the formulation shown in Table 12 below. The obtained cream of Example 12 was subjected to the above stability test and fatigue recovery test.

  The cream of Example 12 had good stability and good fatigue recovery effect.

(Example 13)
A cosmetic solution of Example 13 was prepared according to the formulation shown in Table 13 below. The above-described stability test and fatigue recovery test were performed on the obtained beauty serum of Example 13.

  The cosmetic liquid of Example 13 had good stability and good fatigue recovery effect.

(Example 14)
A cosmetic solution of Example 14 was prepared according to the formulation shown in Table 14 below. The above-described stability test and fatigue recovery test were performed on the obtained beauty serum of Example 14.

The cosmetic liquid of Example 14 had good stability and good fatigue recovery effect.

Claims (9)

(A) the amino acids are valine, leucine and isoleucine,
The manufacturing method of the amino acid melt | dissolution characterized by mix | blending and dissolving the said (A) amino acid and (B) N-acetylglucosamine. Said (A) amino acid is valine 0.1-2.0 mass%, leucine 0.1-4.0 mass%, and isoleucine 0.1-2.0 mass%,
(B) 0.1-4.0 mass% of N-acetylglucosamine,
The method for producing an amino acid lysate according to claim 1.   (C) The manufacturing method of the amino acid melt of Claim 1 or 2 which mix | blends and melt | dissolves anserine.   (D) The manufacturing method of the amino acid melt as described in any one of Claims 1-3 which mix | blends and melt | dissolves an acrylic acid amide type water-soluble thickener.   (E) 1 or more types chosen from the group which consists of PEG-20 sesquistearic acid PEG-20 and dioleic acid PEG-20 methyl glucose, and melt | dissolves the amino acid melt as described in any one of Claims 1-4. Production method.   The method for producing an amino acid solution according to any one of claims 1 to 3, wherein the amino acid solution is a food or drink.   The method for producing an amino acid solution according to any one of claims 1 to 5, wherein the amino acid solution is an external preparation for skin.   The method for producing an amino acid lysate according to any one of claims 1 to 5, wherein the amino acid lysate is a hair restorer. An amino acid lysate produced by the method for producing an amino acid lysate according to any one of claims 1 to 8.