JP2014517323A5

JP2014517323A5 -

Info

Publication number: JP2014517323A5
Application number: JP2014516051A
Authority: JP
Filing date: 2012-06-15
Publication date: 2016-07-21
Anticipated expiration: 2032-06-15

Description

該技術の幾つかの実施形態は、ＧＬＳの下流に配置できるＸＹ型コレクタアームに取り付け可能なコレクタアームアダプタ（例えば図３Ａを参照）をさらに備える。このコレクタアームアダプタの機能は、ひとたび流体フローが常圧条件、例えば流体フローがロボットアームのコレクタ先端部を離れる位置を受けると、発生するエアロゾルを抑制および／または低減することである。例えばコレクタアーム・アダプタは、ＧＬＳを通過した後に、微量の残留量のＣＯ_２（例えば０．００１％から１％、０．０１％から０．１％）がフローストリーム中に残存する場合に適切である。コレクタアームアダプタは、試料をコレクタラックに分散させる先端部を包囲するカバーを含む。コレクタアームアダプタは包囲部を形成して、複合ストリームがＧＬＳを通過した後に非圧縮性流体中に残った微量残留ＣＯ_２の結果として発生し得るいずれの残存エアロゾルも捕捉する。ＧＬＳをコレクタアームアダプタに連結することにより、ストリームがコレクタを出るスペースとコレクタの下のコンテナとの間の、流体フローストリーム周囲の非常に限定された範囲内に、著しく縮小した規模でエアロゾルを十分に封じ込めることができる。幾つかの実施形態において、エアロゾルは完全に低減され、この設計では見ることができない。 Some embodiments of the technology further comprise a collector arm adapter (see, eg, FIG. 3A) that can be attached to an XY-type collector arm that can be positioned downstream of the GLS. The function of this collector arm adapter is to suppress and / or reduce the aerosol that is generated once the fluid flow is subjected to atmospheric conditions, eg, where the fluid flow leaves the collector tip of the robot arm. For example, a collector arm adapter is suitable if a small amount of residual CO ₂ (eg 0.001% to 1%, 0.01% to 0.1%) remains in the flow stream after passing through the GLS. It is. The collector arm adapter includes a cover that surrounds the tip that disperses the sample in the collector rack. The collector arm adapter forms the enclosure, also captures any residual aerosol composite stream may occur as a result of trace residual CO ₂ remaining in the non-compressible fluid after passing through the GLS. By connecting the GLS to the collector arm adapter, the aerosol can be sufficiently reduced on a significantly reduced scale within a very limited area around the fluid flow stream between the space where the stream exits the collector and the container under the collector. Can be contained. In some embodiments, the aerosol is completely reduced and cannot be seen with this design.

Claims

A supercritical fluid chromatography system comprising:
A first pump for pumping a first flow stream containing a compressible fluid;
A second pump for pumping a second flow stream containing a regulator fluid, wherein the second pump is in parallel with the first pump;
A column located in a composite flow stream, wherein the column is located downstream of the first and second pumps, and wherein the composite flow stream is the first flow stream, the second flow stream and the sample. Containing, column,
A detector located downstream of the column;
A gas-liquid separator located downstream of the detector, in order to avoid cross-contamination with sample loss, while retaining most of the sample, to discharge most of the compressive fluid, the remaining flow A gas-liquid separator, which is configured to
A collector arm located downstream of the gas-liquid separator,
A collector arm adapter coupled to the collector arm, the collector arm adapter configured to reduce aerosol when the residual flow is at atmospheric pressure, and a position after the gas-liquid separator Open type XY type collector,
A supercritical fluid chromatography system comprising:

The supercritical fluid chromatography system of claim 1, further comprising an adjustable container rack coupled to the open XY collector.

The supercritical fluid chromatography system according to claim 1, wherein the gas-liquid separator is made of stainless steel, polymer or glass.

The supercritical fluid chromatography system according to claim 1, further comprising a back pressure regulator upstream of the gas-liquid separator.

The supercritical fluid chromatography system according to claim 1, wherein the compressive fluid is carbon dioxide (CO ₂ ).

A method of collecting a plurality of samples by supercritical fluid chromatography (SFC), comprising:
Pumping a first flow stream comprising a compressible fluid;
Pumping a second flow stream comprising an incompressible fluid;
Injecting a sample into the second flow stream;
Combining the first and second flow streams to form a composite flow stream;
Subjecting the flow stream to SFC conditions;
Flowing the composite flow stream through a chromatography column;
Forming a residual flow by removing at least a portion of the compressible fluid from the composite flow stream using a gas-liquid separator ;
and
A collector arm and a collector arm adapter coupled to the collector arm, the collector arm adapter configured to reduce aerosol when the residual flow is at atmospheric pressure, comprising the step of delivering the flow in an open XY type collector method.

The method of claim 6 , wherein the compressible fluid is CO ₂ .

7. A method according to claim 6 , wherein aerosolization of the sample is prevented during collection of the sample to the open XY collector.

The method of claim 6 , wherein removing at least a portion of the compressible fluid from the composite flow stream comprises removing at least 99% of the compressible fluid.