JP2014041096A - Life-style related disease determination support method using saliva examination - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a life-related disease determination support method capable of determining a life-related disease from the daily saliva examination by measuring a prescribed measurement result relating to the saliva and a prescribed measurement result relating to the blood which is different from the prescribed measurement result relating to the saliva respectively so as to previously obtain the correlation between them, and determining a life-related disease from the daily saliva examination using this correlation.SOLUTION: The life-related disease determination support method for determining a life-related disease on the basis of the saliva includes: a first preparation step of obtaining a first correlation between a prescribed component in the saliva and a prescribed component in the blood different from the prescribed component in the saliva; a collection step of collecting the saliva when performing a saliva examination; a component measurement step of measuring the prescribed component in the saliva collected by the collection step; and a first determination step of determining the life-related disease on the basis of the measurement value of the prescribed component in the saliva measured by the component measuring step and the first correlation obtained by the first preparation step.

Description

  The present invention relates to a lifestyle-related disease determination method using a saliva test. More specifically, this is a support method in which a subject can make a determination himself without diagnosing a doctor.

  Conventionally, the redox potential of body fluids (blood, urine, saliva, etc.) is measured, the degree of illness or pain is quantified, the numerical value zone of the health value and disease value is shown, and the health and redox potential values of the subject are measured. It is known to show a health standard (see Patent Document 1).

  It is also known to measure in vivo migration of fat-soluble vitamins and the like from saliva (see Patent Document 2).

  It is also known to detect ischemic disease by measuring the concentration of each human brain type prostaglandin D synthase in blood and saliva (see Patent Document 3).

  Further, it is considered that there are large daily fluctuations, daily fluctuations, and individual differences as problems of the conventional saliva test. In addition, it may be affected by intraoral lesions, and it is considered that it is difficult to determine a standard based only on saliva test data as in blood tests.

JP2002-207037 JP WO2005-085843

  Therefore, it is required to establish a method for collecting saliva in the saliva test, and to set a judgment standard in the saliva test as in the blood test. By the way, the above-mentioned patent documents 1 to 3 measure the same component in saliva and blood, and only look at the correlation between the same components, and measure the components in blood different from the components in saliva. However, the correlation between them is not obtained.

  This invention is made in view of said subject, Comprising: The predetermined | prescribed 1st measurement result (for example, measured value of a predetermined component) regarding saliva differs from the predetermined | prescribed 1st measurement result regarding the said saliva regarding blood A predetermined second measurement result (for example, a measured value of a predetermined component) is measured to obtain a correlation with each other in advance, and a daily-related saliva test is used to determine a lifestyle-related disease (including reserve forces and progress). A lifestyle-related disease determination support method is provided.

As a result of intensive studies in order to solve the above problems, the present invention has been completed. That is, the present invention is a lifestyle disease determination support method for determining lifestyle diseases based on saliva,
A first preparation step for obtaining in advance a first correlation between a predetermined component in saliva and a predetermined component in blood different from the predetermined component in saliva;
When performing a saliva test, a collection process for collecting saliva;
A component measuring step for measuring a predetermined component in the saliva collected in the collecting step;
A first determination step of determining a lifestyle-related disease based on the measured value of the predetermined component in the saliva measured in the component measurement step and the first correlation obtained in advance in the first preparation step.

  According to this configuration, the predetermined first measurement result (measured value of the predetermined component) related to blood, which is different from the predetermined first measurement result (measured value of the predetermined component) related to saliva, and the predetermined first measurement result related to the saliva. ) And the respective correlations (first correlation) are obtained in advance. And daily saliva measurement is performed, and a lifestyle-related disease can be determined from a measurement result and the correlation calculated | required previously. Because it is a saliva test, it is easy for the measurer himself / herself, and it is not necessary to go to the hospital for a blood test, and it is possible to obtain the judgment result of lifestyle-related diseases in a short time (when the blood test cannot be performed in a short time) There is also.)

  In one embodiment of the present invention, the first correlation is that when the predetermined component in the saliva is amylase, the predetermined component in the blood is triglyceride, and the first determination step includes hyperlipidemia. It is the structure which determines. Since the reference value (normal range, abnormal range) of amylase corresponding to the reference value (normal range, abnormal range) of triglyceride for hyperlipidemia is known, hyperlipidemia can be determined from the value of amylase.

  Thus, hyperlipidemia can be easily determined from a saliva test based on the correlation between triglyceride in blood and amylase in saliva. In addition, other dyslipidemia can be determined.

  As one embodiment of the present invention, the first correlation is that when the predetermined component in the saliva is IgA, the predetermined component in the blood is uric acid, and the first determination step determines gout. It is a configuration. Since the reference value (normal range, abnormal range) of IgA corresponding to the reference value (normal range, abnormal range) of uric acid for gout is known, gout can be determined from the IgA value.

  Thus, gout can be easily determined from a saliva test based on the correlation between uric acid in blood and IgA in saliva.

As one embodiment of the present invention, the lifestyle-related disease determination support method comprises:
A second preparatory step for obtaining in advance a second correlation between the pH of saliva and a predetermined component in blood;
A pH measuring step for measuring the pH of saliva collected in the collecting step;
It is the structure further including the 2nd determination process which determines lifestyle-related disease based on the pH of the saliva measured at the said pH measurement process, and the 2nd correlation previously calculated | required by the said 2nd preparation process.

  With this configuration, a predetermined second measurement result (pH value) related to saliva and a predetermined second measurement result related to blood (measured values of predetermined components) different from the predetermined second measurement result related to saliva are measured. Thus, a mutual correlation (second correlation) is obtained in advance. And daily saliva measurement is performed, and a lifestyle-related disease can be determined from a measurement result and the correlation calculated | required previously.

  As one embodiment of the present invention, the second correlation is such that the predetermined component in the blood is triglyceride, and the second determination step determines hyperlipidemia. Since the reference value of pH (normal range, abnormal range) corresponding to the reference value (normal range, abnormal range) of triglyceride for hyperlipidemia is known, hyperlipidemia can be determined from the pH value.

  Thus, hyperlipidemia can be easily determined from a saliva test based on the correlation between triglyceride in blood and the pH of saliva.

As one embodiment of the present invention, the lifestyle-related disease determination support method comprises:
A third preparatory step for obtaining in advance a third correlation between a predetermined component in saliva and blood pressure;
The determination step is configured to further determine lifestyle-related diseases based on the measured value of the predetermined component in saliva measured in the component measurement step and the third correlation obtained in advance in the third preparation step. .

  With this configuration, a predetermined third measurement result (blood pressure) relating to blood, which is different from a predetermined third measurement result relating to saliva, and a predetermined third measurement result relating to blood, which are different from the predetermined third measurement result relating to saliva, are measured. Thus, a mutual correlation (third correlation) is obtained in advance. And daily saliva measurement is performed, and a lifestyle-related disease can be determined from a measurement result and the correlation calculated | required previously.

  As one embodiment of the present invention, the third correlation is such that the predetermined component in the saliva is amylase, and the third determination step determines hypertension. Since the reference value (normal range, abnormal range) of amylase corresponding to the reference value (normal range, abnormal range) of hypertension is known, hypertension can be determined from the amylase value.

  Accordingly, hypertension can be easily determined from a saliva test based on the correlation between blood pressure and amylase in saliva.

As one embodiment of the present invention, the lifestyle-related disease determination support method comprises:
A fourth preparation step for obtaining a fourth correlation between IgA in saliva and pH of saliva in advance;
And a fourth determination step of determining a lifestyle-related disease corresponding to the other by measuring either IgA in saliva or pH of saliva.

  Thereby, based on this fourth correlation, hyperlipidemia can be determined from IgA in saliva regardless of the pH of saliva, while gout can be determined from pH of saliva regardless of IgA in saliva.

As one embodiment of the present invention, the lifestyle-related disease determination support method comprises:
A fifth preparatory step for obtaining in advance a fifth correlation between IgA and amylase in saliva;
And a fifth determination step of determining a lifestyle-related disease corresponding to the other by measuring either IgA or amylase in saliva.

  Thereby, based on this fifth correlation, hyperlipidemia can be determined from IgA in saliva regardless of amylase in saliva, while gout can be determined from amylase in saliva regardless of IgA in saliva. .

  As one embodiment of the present invention, the saliva sample collected in each of the preparation steps is configured to be collected for a predetermined number of days and after a predetermined time after brushing.

  With this configuration, it is possible to obtain a correlation between saliva and blood in consideration of daily fluctuations and daily fluctuations. The predetermined number of days is preferably, for example, 3 days or more, more preferably 5 days or more, and even more preferably 10 days or more in consideration of daily fluctuations (cancelling variation in physical condition). After a predetermined time after brushing, considering daily fluctuations (especially the influence of food and drink), for example, after a meal (morning, noon, night) brushing a few hours (after 1, 2, 3 hours, etc.) In addition, the sampling time may be set at 15:00, and the toothpaste may be set, for example, several hours before that. In order to construct the correlation of a predetermined subject, the correlation may be constructed only with the data of the subject. On the other hand, in order to construct a general saliva-blood correlation, it is necessary to construct with data of more than one person in consideration of individual differences. For example, 10 or more, 15 or more, 20 The above is preferable. In addition, it is preferable that the correlation be different for each man and woman and for each age group.

Another invention is a lifestyle-related disease determination support system for determining a lifestyle-related disease based on saliva,
A correlation storage unit that stores in advance a correlation between a predetermined measurement result related to saliva and a predetermined measurement result related to blood different from the predetermined measurement result related to saliva;
Inspecting the subject's saliva and obtaining a predetermined measurement result related to saliva;
A determination unit for determining lifestyle-related diseases based on a predetermined measurement result relating to saliva obtained by the saliva test unit and a correlation stored in the correlation storage unit;
And an output unit that outputs a result determined by the determination unit.

  According to this configuration, daily saliva measurement is performed, lifestyle-related diseases are determined from the measurement results and correlations obtained in advance, and output (displayed on a monitor, transmitted to an external device, stored in a storage medium, printed Can be included). Examples of the “predetermined measurement result regarding saliva” include pH, amylase, and IgA. Examples of the “predetermined measurement result related to blood” include triglyceride, blood pressure, and uric acid.

  The correlation storage unit is, for example, a storage medium (various media), a computer HDD, or the like. The determination unit can be configured by, for example, a hardware configuration such as a CPU and a memory of a computer and a combination configuration with a software program, a dedicated circuit, firmware, and the like. A saliva test | inspection part is a pH measuring device and an amylase measuring device, for example, transmits measurement data to a determination part, and can implement the said determination based on the measurement data which the determination part received. Alternatively, various measurement data (pH, amylase, IgA) are input using the input unit of the computer, or various measurement data are received from the communication unit, and the determination unit as one component of the computer performs the above determination. be able to.

  Although the present embodiment will be described below, the present invention is not limited to the following contents.

(Creation of correlation)
First, the correlation between saliva and blood is constructed. Obtain health checkup data and interview data from the saliva provider and confirm that they are healthy. The blood sample and various measurement values use the measurement values of the health checkup. The method for collecting saliva is as follows. First, brush your teeth after lunch, then take a saliva sample at 15:00, 2 hours later. This is done for 5 consecutive days.

(Saliva test)
The collected saliva sample is measured. Various pH measuring devices can be used for pH measurement, and examples thereof include “Twin pH” manufactured by Horiba. In addition, various amylase measuring instruments can be used for amylase measurement, for example, Nipro's “saliva amylase monitor”. In IgA measurement, the concentration of antibody or antigen contained in saliva by ELISA is quantitatively detected. The ELISA method is a general method. Saliva samples are collected for 5 days from 24 people by the above collection method. Extremely deviating data can not be used. Table 1 shows the average pH, average amylase value, and average IgA value of the 5-day saliva sample. This is because the amount of saliva sample was small and could not be measured.

(Correlation between saliva test results and blood test results)
First, the Pearson correlation coefficient is tested for the entire saliva sample (average value over 5 days). A correlation coefficient that is an index for measuring the degree of association between two variables is obtained, and the significance of the correlation coefficient is tested. That is, the null hypothesis: “no correlation between two variables” is tested by testing the population correlation coefficient ρ = 0. Also, the 100 (1-α)% confidence interval of the population correlation coefficient ρ is displayed, and when 0 is not included in the confidence interval of ρ, it means that ρ≈0, that is, the test is significant, and the bivariate It can be seen that there is a correlation between them. Specifically, in the correlation result and determination, a correlation coefficient is calculated, a P value is calculated, and a confidence interval is calculated. If the risk value is within 5% in the P value, it is determined that there is a correlation significance.

  Table 2 shows data of pH and triglyceride, amylase and blood pressure (upper value), amylase and triglyceride, IgA and uric acid, in which a significant correlation was recognized.

  As shown in Table 2, there was a significant positive correlation between pH and triglyceride, a significant negative correlation between amylase and blood pressure (upper value), and a significant negative correlation between amylase and triglyceride, There is a significant negative correlation between IgA and uric acid. Each saliva data uses an average value for 5 days.

(Correlation between saliva test items)
Table 3 shows data of IgA and pH, and IgA and amylase, in which correlations were recognized.

  As shown in Table 3, there is a clear negative correlation between IgA and pH, and there is also a clear positive correlation between IgA and amylase.

(Correlation between IgA and uric acid considering other measured values)
Next, regarding the IgA and the uric acid value, the correlation in the following extracted sample group is shown in Table 4.

  Table 4 shows the correlation between IgA and uric acid levels in people with high pH, correlation between IgA and uric acid levels in people with low amylase, correlation between IgA and uric acid levels in people with low redox potential, blood glucose levels Shows the correlation between IgA and uric acid levels in the low group. The average value of all data of each index was divided into high group and low group on the boundary. In the high pH group, the correlation coefficient between IgA and uric acid was “−0.866”, and the absolute value approached 1, and a higher correlation was observed in the high group than in the low group. In the low group of amylase, the correlation coefficient between IgA and uric acid was “−0.941” and the absolute value approached 1, and a higher correlation was observed in the low group than in the high group. In addition, the blood glucose level is divided into a high group and a low group on the basis of the average blood glucose level of the whole blood sample. In the high blood glucose group, the correlation coefficient between IgA and uric acid is “−0.843” and the absolute value is 1. The higher group showed a higher correlation than the lower group. In addition, the average value of the redox potential of the whole saliva sample is divided into a high group and a low group, and in the low group of the redox potential, the correlation coefficient between IgA and uric acid is “−0.979” and an absolute value. Was close to 1, and a higher correlation was observed in the low group than in the high group. The oxidation-reduction potential is measured by an electrochemical measurement method using a silver chloride electrode (reference electrode) and a platinum electrode (working electrode).

(Judgment flow)
From the above results, the correlation is obtained in advance. Next, in order to examine the saliva of the subject, a saliva sample is collected. Saliva collection is preferably performed in the same manner as described above, and preferably several hours after dentifrice on the day to be examined (for example, 15:00). Next, the collected saliva is measured. For example, the pH of the saliva sample is measured with a pH meter. Further, amylase in the saliva sample is measured with an amylase measuring instrument. Moreover, IgA is measured by ELISA method. Next, lifestyle-related diseases are determined based on the various measured values measured above and the correlation obtained in advance. The determined result is output. Examples of the output include monitor display, saving to another storage medium, printing, transmission to another information processing apparatus, and the like.

  In this embodiment, hyperlipidemia can be determined from pH or amylase. Also, the same hyperlipidemia can be determined based on pH and amylase, but if hyperlipidemia is determined only by one of the indicators, it is determined to be hyperlipidemic at a low level. When hyperlipidemia is determined by both indices, it can be determined that the hyperlipidemia is at a high level. That is, the determination can be made step by step using two different indexes.

  Moreover, ventilation can be determined from IgA, and it can be determined from amylase whether it is hypertension.

  Further, ventilation can be determined based on pH or amylase from the correlation between pH and IgA or amylase and IgA. The same gout can be determined based on pH or amylase (via correlation with IgA), but if ventilation is determined only with one of the indicators, it is determined that the ventilation is at a low level. When gout is determined by both indicators, it can be determined that the ventilation is at a high level. According to this, ventilation can be determined without measuring IgA.

  Hyperlipidemia can also be determined from IgA based on the correlation between pH and IgA or amylase and IgA. That is, hyperlipidemia can be determined based on the three indices.

As described above, according to the present embodiment, it is possible to easily determine a lifestyle-related disease from the measurement result related to saliva and the correlation between blood and saliva obtained in advance, regardless of the blood test.

Claims (10)

A lifestyle-related disease determination support method for determining lifestyle-related diseases based on saliva,
A first preparation step for obtaining in advance a first correlation between a predetermined component in saliva and a predetermined component in blood different from the predetermined component in saliva;
When performing a saliva test, a collection process for collecting saliva;
A component measuring step for measuring a predetermined component in the saliva collected in the collecting step;
A life including a measurement value of a predetermined component in saliva measured in the component measurement step and a first determination step of determining a lifestyle-related disease based on a first correlation obtained in advance in the first preparation step Habitual disease determination support method. The first correlation is that when the predetermined component in the saliva is amylase, the predetermined component in the blood is triglyceride,
The lifestyle-related disease determination support method according to claim 1, wherein the first determination step determines hyperlipidemia. The first correlation is that when the predetermined component in the saliva is IgA, the predetermined component in the blood is uric acid,
The lifestyle-related disease determination support method according to claim 1 or 2, wherein the first determination step determines gout. The lifestyle-related disease determination support method includes:
A second preparatory step for obtaining in advance a second correlation between the pH of saliva and a predetermined component in blood;
A pH measuring step for measuring the pH of saliva collected in the collecting step;
The second determination step of determining a lifestyle-related disease based on the saliva pH measured in the pH measurement step and the second correlation obtained in advance in the second preparation step. The lifestyle-related disease determination support method according to any one of the above. In the second correlation, the predetermined component in the blood is triglyceride,
The lifestyle-related disease determination support method according to claim 4, wherein the second determination step determines hyperlipidemia. The lifestyle-related disease determination method includes:
A third preparatory step for obtaining in advance a third correlation between a predetermined component in saliva and blood pressure;
The determination step further determines a lifestyle-related disease based on a measured value of a predetermined component in saliva measured in the component measurement step and a third correlation obtained in advance in the third preparation step. The lifestyle-related disease determination support method according to any one of 1 to 5. In the third correlation, the predetermined component in the saliva is amylase,
The lifestyle-related disease determination support method according to claim 6, wherein the third determination step determines hypertension. The lifestyle-related disease determination support method includes:
A fourth preparation step for obtaining a fourth correlation between IgA in saliva and pH of saliva in advance;
The 4th determination process which further determines the lifestyle-related disease corresponding to the other by measuring any one of IgA in saliva or the pH of saliva in any one of Claims 1-7. The lifestyle-related disease determination support method described. The lifestyle-related disease determination support method includes:
A fifth preparatory step for obtaining in advance a fifth correlation between IgA and amylase in saliva;
The method according to any one of claims 1 to 8, further comprising: a fifth determination step of determining a lifestyle-related disease corresponding to the other by measuring either IgA or amylase in saliva. Lifestyle-related disease determination support method. The lifestyle-related disease determination support method according to any one of claims 1 to 9, wherein the saliva sample collected in each of the preparation steps is collected after a predetermined time after brushing for a predetermined number of days.