JP2013023496A - Inhibitory device for fat droplet and cellulite, drug enhacing lipid inhibitory effect, and provitamin-c formulation for hyperthermia for cellulite prophylactic treatment, fat droplet inhibition and lipid metabolism improvement - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an inhibitory device for fat droplets and cellulite, the device capable of significantly increasing the inhibitory effect on fat droplets and cellulite by combining a vitamin-C agent and high-frequency hyperthermia device, and suppressing cell death due to heating to the minimum, and giving mild hyperthermia treatment to a person to be treated without any pains by heating, and to provide a drug enhancing lipid inhibitory effect.SOLUTION: The drug enhancing lipid inhibitory effect includes vitamin-C and/or a provitamin-C for hyperthermia for cellulite prophylactic treatment, fat droplet inhibition and lipid metabolism improvement.

Description

  The present invention provides a lipid droplet / cellulite suppressing apparatus capable of improving cellulite / lipid metabolism with high safety that can minimize cell death due to high-frequency heat, preferably a cell survival rate of 97% or more, and a lipid suppressing effect. The present invention relates to a provitamin C preparation for hyperthermia for potentiating drugs, cellulite prevention treatment, lipid droplet suppression and lipid metabolism improvement.

  2. Description of the Related Art Conventionally, a new composition capable of suppressing undesired expression of tissues and cells related to lipid droplets and its effect and a drug for the same have been known.

Conventionally, as a lipid droplet formation inhibitor, a lipid droplet formation inhibitor comprising one or more fullerene complexes modified or clathrated with a fullerene hydroxide and an organic compound as an active ingredient ( There is an invention of claim 1 (Patent Document 1: Japanese Patent Laid-Open No. 2008-222645).
The same document 1 describes the following in claims 2 and below. 2. The lipid droplet formation inhibitor according to claim 1, wherein the hydroxylated fullerene is a fullerene having a hydroxyl group bonded at 30 to 40 per molecule or a derivative thereof (claim 2). The fullerene complex is a complex of one or more of fullerenes and fullerene derivatives and one or more organic compounds of organic oligomers, organic polymers, cyclodextrins and crown ethers and related compounds. The lipid droplet formation inhibitor according to claim 1 (claim 3). 4. The lipid droplet formation inhibitor according to claim 3, which is a fullerene complex included in a ratio of 1.5 to 2.0 mol / mol with γ-cyclodextrin (claim 4). A protective agent for cytotoxicity derived from lipid droplets, comprising the inhibitor according to any one of claims 1 to 4 (claim 5). An adipocyte differentiation inhibitor comprising the inhibitor according to any one of claims 1 to 4 (claim 6).

JP 2008-222645 A

Patent Document 1 realizes suppression of formation of highly active lipid droplets that can replace conventional retinoids, thereby effectively preventing the occurrence of cell damage derived from lipid droplets and suppressing differentiation of fat cells themselves. It is described that it is useful as skin care, cosmetics, skin active agents and the like.
However, even if skin care, cosmetics, etc. containing a lipid droplet formation inhibitor are marketed, it cannot be said that this alone can be expected to have a sufficient effect for suppressing lipid droplet formation and cellulite.

  In addition, with conventional high-frequency thermal equipment, it has been said that a higher thermal effect can be obtained by setting a high temperature within the range where the patient can withstand the heat, but it is difficult for the patient to suffer from the heat. It was a thing.

  Furthermore, the fact that heat seems to suppress fat droplets and cellulite (uneven fat mass on the skin surface) has been vaguely known from experience, but the specific heat conditions have not been elucidated. However, analyzing the human body itself for the purpose of elucidating it is difficult to ensure a statistically sufficient number of cases, and the human body characteristic conditions are the same between the control group and the test group. In addition, it is difficult to analyze the increase and decrease of cellulite and fat droplets in the human body from the viewpoint of accuracy and sensitivity.

  The present invention has been made in view of the above points, and the object of the present invention is a provitamin C agent (which is absorbed into the body and then undergoes a chemical change to become vitamin C. It is also called a precursor. )) And a high-frequency heating device, it is possible to greatly improve the effect of suppressing lipid droplets and cellulite, and to minimize the death of cells due to heat. Is to provide a lipid droplet / cellulite suppression device and a lipid suppression effect enhancer capable of performing mild thermothermal treatment, and a provitamin C preparation for thermotherapy for cellulite prevention treatment, lipid droplet suppression, lipid metabolism improvement .

  The apparatus for suppressing lipid droplets and cellulite according to the present invention includes a means for administering a provitamin C agent to a target body part, an electrode for contacting the treatment site, and the back of the treatment target body contacting the electrode With a resistive electrode as a return electrode and a temperature adjustment unit in the temperature range of 37-41 ° C. under a high frequency of 0.45 MHz, and the heat treatment time per time is set to 1-30 minutes It is characterized by comprising a heat treatment time adjusting unit for performing the heat treatment, and a heat administration means having a total interval of 6 hours to 1 week.

  The provitamin C agent is ascorbic acid-2-0-phosphate / ascorbic acid-2-0-α-D-glucoside / ascorbic acid-2-0-phosphate-6-0-palmitate / ascorbic acid. -2-0-phosphate-6-0- (2-hexyldecanoic acid) ester / ascorbic acid-2,3,5,6-0- (2′-hexyldecanoic acid) tetraester, ascorbic acid-6-0- One or two or more members selected from the group consisting of palmitic acid ester and ascorbic acid-6-0-stearic acid ester are used.

  The fat inhibitory effect enhancer according to the present invention is characterized by containing the above-mentioned provitamin C agent and / or vitamin C for heat treatment for cellulite prevention treatment, lipid droplet suppression, and lipid metabolism improvement. In particular, in the case of vitamin C, it is composed of hydrogenated soybean lecithin / cholesterol, and the vitamin C has enhanced skin permeability as it is ascorbic acid by liposomes prepared from an ultrasonic device / extruder. It is characterized by.

  The provitamin C preparation and / or vitamin C preparation for thermotherapy for cellulite prevention treatment, lipid droplet suppression, and lipid metabolism improvement according to the present invention are obtained without decomposing the provitamin C preparation and / or vitamin C even in the heat treatment. It is characterized by containing a thermal stabilizer to be retained.

  The provitamin C preparation and / or vitamin C preparation for thermotherapy for cellulite prevention treatment, lipid droplet suppression and lipid metabolism improvement according to the present invention is selected from the group consisting of mannitol, corn starch, chitin and chitosan as a thermostabilizer It contains one or more selected substances.

  The fact that heat seems to suppress fat droplets and cellulite (skin irregularities on the skin surface) has been vaguely known from experience, but the specific heat conditions have not been elucidated. Analyzing the human body itself for the purpose of clarifying it is difficult to ensure a statistically sufficient number of cases, and to make the human body characteristic conditions identical between the control group and the test group In addition, it is difficult to analyze the increase and decrease of cellulite and fat droplets in the human body from the viewpoint of accuracy and sensitivity.

  The present invention uses preadipocytes and / or adipocytes that play a central role in the formation of lipid droplets and cellulite, and further includes a three-dimensional human skin tissue model incorporating these preadipocytes and / or adipocytes, Using a human skin-extracted tissue piece and a Yucatan mini-pig skin-extracted tissue piece, the test group and the control group were manipulated under the same skin characteristic conditions, respectively, and statistically sufficient A number of application examples were analyzed, and the optimum conditions for suppressing lipid droplets and cellulite were determined.

As a result, it was found that the combination of various provitamin C agents in addition to warm heat treatment alone makes the suppression of fat droplets and cellulite remarkable as a synergistic effect, as compared with the single use of the heat treatment apparatus.
Further, a means for performing heat treatment at intervals of a certain period of time, and a combination means for administering the specific provitamin C agent found in the present invention to the target body part at a specific concentration upon application of each heat treatment means include lipid droplets, It has been found by the present invention that it is highly effective in suppressing cellulite.

  Furthermore, since the fat inhibitory effect enhancer according to the present invention contains the above-mentioned provitamin C agent and / or vitamin C, it effectively functions in the treatment for heat for cellulite prevention treatment, lipid droplet suppression, and lipid metabolism improvement. It becomes.

  Furthermore, the provitamin C agent and / or vitamin C preparation for thermotherapy for cellulite prevention treatment, lipid droplet suppression, and lipid metabolism improvement according to the present invention is decomposed in the above-mentioned provitamin C agent and / or vitamin C even in the heat treatment. It is possible to contribute as a thermal stabilizer that is retained without being.

  The provitamin C preparation and / or vitamin C preparation of the present invention contains at least one substance selected from the group consisting of mannitol, corn starch, chitin and chitosan as a heat stabilizer, thereby preventing cellulite. -Suitable for hyperthermia for lipid droplet suppression and lipid metabolism improvement.

It is a perspective view which shows the fat droplet and a cellulite suppression apparatus by combined use of a provitamin C agent and a high frequency heating apparatus using the Yucatan mini-pig skin-extracted tissue piece. It is a state diagram which shows the case where the test piece A, B, C which administered the provitamin C agent of FIG. 1 is administered with high-frequency heat for 5 minutes under the temperature condition of 41 ° C. using the heat treatment apparatus according to the present invention. . It is a state figure which shows the change of the lipid droplet accumulation amount with respect to APS dosage. Micrographs of lipid droplets, the upper left photo shows the case of no administration of provitamin C and heat treatment apparatus, the upper right photo shows the case of administration of 0.03% APS only, and the lower left photo shows no APS. The case where only a heat treatment apparatus is applied by administration is shown, and the lower right photograph shows the state of fat droplets when the APS of 0.03% concentration and the heat treatment apparatus are applied. After 30,000 cell groups are left for 2 days, APS administration and a thermal treatment apparatus are applied, only the thermal treatment apparatus is applied after 6 hours, and the operation of applying the APS administration and the thermal treatment apparatus after 18 hours is repeated. It is explanatory drawing made into the conditions of photomicrograph photography. It is a microscope picture which shows the relative ratio of fat droplet accumulation amount. It is explanatory drawing made into the conditions of the microscope picture photography of the test piece for imaging | photography the microscope picture of FIG. It is a microscope picture which shows the state of the lipid droplet at the time of not administering APS of 0.03% density | concentration and not applying a heat processing apparatus, the case where any one is employ | adopted, and using together. It is explanatory drawing which shows the motion of the electric current of a capacitive electric transfer system. It is explanatory drawing which shows the energy distribution added between the asymmetrical electrodes. It is a rear view of the MD530 Indiver CRet System. It is a front view of indiver CRet System of MD530. It is explanatory drawing of the display part of the controls etc. displayed on the Indiva CRet System front surface of MD530.

Lipid droplet / cellulite suppression and cell death protection in adipocytes / preadipocytes were performed.
When the mesenchymal preadipocytes OP9 are cultured and replaced with the insulin-containing KSR medium from the normal medium, differentiation into adipocytes is initiated. At this time, the Capacitive-Resistive Electric-Transfer apparatus (Indiva Corp.) Electrical characteristics corresponding to Level 5, Indicator 5, 20 sec were set using a body heat apparatus of Japan product: MD530) (CRet System). The room temperature was shifted from 26.5 ° C. to 41 ° C., after which it was set to Level 2-3 of the same apparatus. Adipocytes were kept at 41 ° C. for 1 min. At this time, ascorbic acid-2-O-phosphate sodium salt (APS) as a provitamin C agent was administered at a concentration of 1 mM (300 ppm, 0.03 wt%) and repeated twice every 12 hours a day. The test group for 3 days was compared with the non-administered control group.
Lipid droplets inside the cells are stained with oil red o dye, rinsed with 10% aqueous methanol solution, and rinsed thoroughly until the rinse waste liquid has zero absorbance at 530 nm until it reaches zero. After extinguishing the oil red o dye on the cell surface, take a picture with a phase contrast microscope, extract the oil red o dye in the cell, and remove the intracellular lipid droplets with an absorption plate reader and spectrophotometer at 530 nm. Quantified. Cell death and cell survival were stained with propidium iodide and calcein aminomethoxyester, respectively, and the fluorescence was evaluated with a fluorescence plate reader.

  The MD 530 used in the present invention is a 0.45 MHz high-frequency heating device that warms human body cells deeply through circulation to a treatment site. Deep heating is performed using capacitive and resistive electrodes. When the capacitive mode is adopted, the surface of the stainless steel electro-rode circular disc is coated with plastic so that it does not directly touch human skin. The electrode acts as a condenser that transmits high-frequency energy to cells, and warms the treatment site. The transmitted high frequency energy is transmitted toward the plate-type return electrode. On the other hand, in the resistive mode, a high-frequency current is passed directly to the human body cell through the RES electrode (made of stainless steel) placed at the treatment site. This current is conducted to the return electrode. When high-frequency energy is permeated into the cell, the cell becomes a resistor, and the temperature of the tissue is increased intensively, and deep heating is performed. This device can measure and monitor the intensity of current flowing to the patient, the apparent power applied to the patient (capacitive mode), active power (resistive mode), and the resistance of the electrode and the patient. .

  FIG. 11 is a rear view of the MD 530 indiver CRet System, where 1 is a power switch, 2 is a fuse holder, 3 is a power socket, 4 is a grounding jack, 5 is a cooling fan, and 6 is a specification label. 12 is a front view of the MD 530, 7 is an LCD screen, 8 is a keypad, 9 is a capacitive output, 10 is a return electrode output, and 11 is a resistive output. FIG. 13 is an explanatory diagram of a display section of controls displayed on the front surface of the MD 530. 12 is a standby indicator lamp, 13 is an ON / OFF key, 14 is a menu key, 15 is a capacitive mode key, a resistive mode key, 17 Is a reset key, 18 is a start / stop key, 19 is a time increase key, a time decrease key, and 21 is an output increase / decrease dial knob.

  The impedance for capacitive mode output is calculated to correspond to the impedance (1,000 ohms) of the electrode placed on the skin tissue. By this method, a large amount of energy can be transmitted. The direction of circulating high frequency energy to the tissue through the electrodes is shown in FIG. The energy from the MD 530 is transmitted to the tip of B (the lowest part of the electrode) through the metal axis A. The energy is reduced because it is separated from the tissue by the insulator portion of C, and is transferred to the tissue capacitively across the entire surface of the electrode. The tissue D fixes a plurality of infinite resistances totaling 1,000 ohms, and acts like a second plate due to the nature of the capacitor. A dotted line E in FIG. 9 is defined as the second plate, which serves as a capacitor. A resistance equivalent to 1,000 ohms is actually generated between point B and point E, and the temperature rises at that point. The depth of the resistance depends on the power of this device (corresponding to level adjustment) and the size of the electrode.・ It depends on the characteristics of the tissue at the treatment site and the treatment time. In particular, in the present invention, the patient does not feel pain due to the heat for the patient, and the heat treatment can be performed by a gentle heat treatment, that is, a temperature setting in a range of 37 to 41 ° C.

The results are shown below under the above conditions.
(1) By the differentiation induction treatment into adipocytes, accumulation of fat droplets (stained in red; about 20 per cell) was observed around the cell nucleus. Cell viability was 99.3 ± 0.4%.
(2) The CRet thermal apparatus at 41 ° C. reduced the lipid droplet accumulation amount by 22% even without the provitamin C agent. The application of the CRet System thermal apparatus suppressed the accumulation of fat droplets. Although cell death was a little 0.8 ± 0.2%, it was not remarkable.

(3) Provitamin C agent (ascorbic acid derivative) APS (ascorbic acid-2-O-phosphate sodium salt) 300 ppm (0.03 wt%, 1 mM) in the case where the above-mentioned thermal apparatus is not applied Administration at 37 ° C. caused lipid droplet accumulation around the cell nucleus, but suppressed lipid droplet accumulation by 20%. Cell death due to the application of APS or CRet thermostat (1 min) was slightly seen as 0.1 ± 0.0%, but was negligible.

(4) The combined use of 300 ppm of APS and the CRet System heat apparatus suppressed lipid droplet accumulation by 54%. The combined use of the ascorbic acid derivative APS and the CRet System heating device significantly increased the lipid droplet suppression effect. The majority of cells were without lipid droplets. Although lipid droplets accumulated around the cell nucleus, APS administration suppressed lipid droplet accumulation throughout the cells, and this suppression was further increased by the combined use of the CRet System thermal apparatus. Cell viability was maintained at 99.0 ± 0.6%. Vitamin C converted from APS promotes the enzyme trimethyl lysine dioxygenase & γ-butyrobetaine dioxygenase that synthesizes the fat burning accelerator carnitine, and suggests that lipid droplet fluidization and dispersion using the CRet System thermal apparatus have been successful. It is.

(5) As a conventional method, as a result of testing a comparative example in which heating was carried out for 60 minutes each at four points of 42, 43, 44, and 45 ° C. with a normal body heating device, At temperature, the inhibition was 8.9, 11.6, 10.4, 5.2%, and cell death was 18.1 ± 4.7, 27.0 ± 5.9, 41.3 ± 11, respectively. 0.0, 61.8 ± 5.5%. Severe use of a hyperthermia device leads to decreased activity of mitochondrial dehydrogenase mainly composed of succinate dehydrogenase, which is an indicator of cell damage and cell survival. In addition, cell morphology by transmission electron microscopy is an indicator of active cell metabolism. Disappearance of microvillus, rupture of cell membrane, shrinkage (cell atrophy), pycnosis (nuclear condensation), and calorixis (nuclear fragmentation) were observed. These suggest the possibility that the application of the conventional severe thermal apparatus was carried out at the expense of causing cell death in a wide range.

Lipid droplet / cellulite suppression and cell death protection were carried out in a 3D human skin tissue model incorporating preadipocytes.
The dermis composed of type I collagen containing human dermal fibroblasts OUMS-36, the basement membrane composed of matrigel containing type IV collagen, laminin, entactin and fibronectin, and further, the mesenchymal system The amount of lipid droplet formation obtained by adding preadipocytes OP9, on which each of the epidermis composed of human skin keratinocytes HaCaT is layered, and finally the stratum corneum is formed by air lift A three-dimensional human skin tissue model for evaluation was prepared, the same test as in Example 1 was performed, and lipid droplet / cellulite suppression and cell death protection were evaluated. In addition, the skin tissue piece was ultrathin with a thickness of 4 μm using a cryostat. In the cross-sectional structure of the skin tissue in which a slice was prepared and stained with oil red o dye, the ratio of the area of the fat drop to the cross-sectional area was expressed as NI It was analyzed by -Image and Image-J software. The cell death level was evaluated by hematoxylin-eosin staining from the ratio of the degenerated cells to the total number of cells. Furthermore, the uneven shape of the skin surface was also photographed with a scanning electron microscope. For the uneven shape on the skin surface, a replica using silicone rubber was prepared, and the degree of cellulite was evaluated by converting the unevenness into a line histogram.

As a result, as a comparative example, a single application of the CRet System heating device at 41 ° C. (shift up from room temperature 34 sec, temperature parallel time after reaching set temperature 5 min, shift down to room temperature 3 min 14 sec: 5 min application ”) reduced lipid droplet accumulation by 22.4 ± 3.7% without provitamin C agent. The effect of suppressing lipid droplet accumulation by the single application of the CRet System heating device was significantly shown.
As another comparative example, when the above-mentioned thermal apparatus at a concentration of 300 ppm (0.03 wt%) of provitamin C agent (ascorbic acid derivative) APS (ascorbic acid-2-O-phosphate sodium salt) is not applied. Administration at 37 ° C. showed lipid droplet accumulation around the cell nucleus, but suppressed lipid droplet accumulation by 16.1 ± 2.9%. Cell death due to APS alone administration or CRet System heating device (5 min application) alone was 0.1 ± 0.0% and 2.2 ± 0.3%, respectively, which were negligible or minimal.

  On the other hand, in the present invention, 300 ppm administration of APS and CRet System heat apparatus are combined, or as a heat stabilizer, mannitol 500 ppm, corn starch 800 ppm, squid muscle (medium bone) chitosan (chitosan component 83 to 97%, chitin component 3 ˜17%; chitin / chitosan prepared by treatment such as deacetylation using β-type chitin as a starting material) 300 ppm administration of APS added with 350 ppm and combined use of CRet System thermal apparatus, 54.7 It was suppressed by ± 4.6% or 73.3 ± 5.8%. The combined use of the ascorbic acid derivative APS and the CRet heating device (5 min application) increased the lipid droplet suppression effect, and this effect was further promoted by the addition of a thermal stabilizer. In all of the examples of the present invention, the majority of the cells in which fat droplets were hardly detected accounted for. Lipid droplets accumulated around the cell nucleus, but APS administration suppressed lipid droplet accumulation throughout the cell, and this suppression was further increased by the combined use of the CRet System thermal apparatus, and was further marked by the thermal stabilizer. It will be. The cell death rates were also negligible levels of 0.3 ± 0.0% and 0.2 ± 0.0%, respectively.

Lipid droplets / cellulite suppression and cell death protection were performed in the skin excised tissue piece under the ear of a 48-year-old Japanese woman who submitted informed consent incorporated into the modified Bronov diffusion chamber.
After completion of the lipid droplet suppression test, the skin tissue piece isolated from the chamber was prepared with an ultrathin section having a thickness of 4 μm using a cryostat, and the lipid droplet / cellulite suppression and cell death protection were evaluated in the same manner as in Example 2.

As a result, as a comparative example, a single application of the CRet System heating device at 41 ° C. (shift up from room temperature 33 sec, temperature parallel time after reaching the set temperature 5 min, shift down to room temperature 3 min 27 sec: 5 min application)) reduced lipid droplet accumulation by 19.1 ± 2.9% even without provitamin C. The effect of suppressing lipid droplet accumulation by the single application of the CRet thermal apparatus was significantly shown.
As another comparative example, in the case where the above-mentioned heating apparatus at a concentration of 300 ppm (0.03 wt%) of provitamin C agent (ascorbic acid derivative) APS (ascorbic acid-2-O-phosphate sodium salt) is not applied. Administration at 37 ° C. caused lipid droplet accumulation around the cell nucleus, but suppressed lipid droplet accumulation by 12.0 ± 3.5%. Cell death due to APS administration or APS-free CRet System heating device (5 min application) alone was 2.0 ± 0.4% or 6.9 ± 0.8%, respectively, at negligible or negligible levels.

  On the other hand, in the present invention, 300 ppm administration of APS and CRet System thermal apparatus are combined, or as thermal stabilizer, mannitol 500 ppm, corn starch 800 ppm, squid muscle (medium bone) chitosan (chitosan component 83 to 97%, chitin component 3 to 3%) 17%; chitin / chitosan prepared by treatment such as deacetylation using β-type chitin as a starting material) 300 ppm of APS added with 350 ppm and combined use of CRet System heating device were 54.8 ± 12.6%, respectively. Alternatively, 70.4 ± 6.3% lipid droplet accumulation was suppressed. The effect of suppressing lipid droplets was clarified by the combined use of APS of provitamin C and the CRet System thermal apparatus (5 min application), and when the thermal stabilizer was used in combination, the lipid droplet suppressing effect became significant. The majority of the cells were almost undetectable lipid droplets. Lipid droplets accumulated around the cell nucleus, but APS administration suppressed lipid droplet accumulation throughout the cell, and this suppression was further increased by the combined use of the CRet System heating device, and the promotion effect by the thermal stabilizer was also increased. Admitted. The cell death rate was also negligible at 1.1 ± 0.2% or 1.7 ± 0.2%, respectively.

Fat mass and cellulite in clinical trials were evaluated by MRI of 24 healthy Japanese women aged 26-56 years old who obtained informed consent in advance at the back of the thigh and the thickest part of the buttocks. And the skin surface shape of the said part was evaluated by the skin cast skin surface replica method as cellulite skin surface unevenness degree.
Before the test, 24 people were divided into 3 groups of 8 people each so that the subcutaneous fat rate, cellulite skin degree and average age were not biased, 3 times each week, group A was APS 300 ppm without thermal stabilizer. Was applied to the thighs and buttocks at 1 mL / cm ² and the Cret System heating device was applied for 10 minutes and 15 minutes, respectively. Group B of the present invention was mannitol 500 ppm, corn starch 800 ppm, squid muscle (medium bone) as a thermal stabilizer. ) Chitosan (chitosan component 83 to 97%, chitin component 3 to 17%; chitin / chitosan prepared by a process such as deacetylation using β-type chitin as a starting material) APS300ppm added with 350ppm was applied in the same manner. The system was applied in the same manner, and the control group C was continuously tested for 3 weeks each as the CRet System application only. The MRI image data was analyzed with high accuracy by a measurement algorithm that uses 0-Gauge software to identify the boundary line with visceral fat, automatically discriminate subcutaneous fat, and calculate the fat area. Cellulite skin irregularities are 10 cm square and 15 cm square for each part of the thigh and buttocks, and the skin surface shape is duplicated by skin casting. Large (vertical) amplitude was analyzed with Image-j software as a measurement target.

  As a result, the subcutaneous fat area ratio was 84.5 ± 7.4% for the A group and 68.9 ± 4.0% for the B group after the test on the basis of the values before the test on the average of the 8 subjects. , C group is 92.5 ± 6.2%, cellulite skin roughness is 73.1 ± 4.8% for group A, 44.5 ± 2.0% for group B, 88 for group C .9 ± 9.3% and all three groups compared to before the test, both subcutaneous fat and cellulite shape decreased, and the improvement effect was significantly recognized in the A, B and C3 groups. Among these, the improvement effect of A group is large compared with C group, and it shows that the combined use effect by APS of a provitamin C agent is excellent, and B group has both subcutaneous fat and cellulite shape compared with A group. In both cases, a more remarkable improvement effect was observed, and it was verified that the effect of adding the stabilizer to the heat of APS was remarkable.

  The present invention can greatly contribute to the fields of aesthetic beauty industry, dermatology, and the like, or the medical device / beauty device industry.

A Metal shaft B Electrode C Insulator part E Second plate 9 Capacitive output 10 Return electrode output 11 Resistive output 15 Capacitive mode key 16 Resistive mode key 19 Time increase key 20 Time decrease key

Claims (4)

  A lipid inhibitory effect potentiator comprising the above-mentioned provitamin C agent and / or vitamin C for heat treatment for cellulite prevention treatment, lipid droplet suppression, and lipid metabolism improvement.   The above-mentioned provitamin C agent and / or vitamin C contains a thermal stabilizer that retains without decomposition even in thermal treatment for thermotherapy for cellulite prevention treatment, lipid droplet suppression, and lipid metabolism improvement. The lipid inhibitory effect enhancer according to claim 3.   5. The lipid inhibitory effect enhancer according to claim 3 or 4, wherein the thermal stabilizer contains one or more substances selected from the group consisting of mannitol, corn starch, and chitin / chitosan.   The above-mentioned vitamin C is composed of hydrogenated soybean lecithin / cholesterol, and is a vitamin C whose skin permeability including ascorbic acid is enhanced by liposomes prepared from an ultrasonic device / extruder. 3. The lipid inhibitory effect enhancer according to 3, 4 or 5.