JP2012078094A - Inspection object acceptor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an inspection object acceptor from which a liquid does not outflow in the case where the inspection object acceptor is attached on an inspection apparatus which vertically holds the inspection object acceptor.SOLUTION: In a plate-like member 2 of an inspection object acceptor 1, there are formed: a liquid sump portion 5 including a recessed portion having a predetermined depth; a flow channel 7 through which liquid outflows from the liquid sump portion 5; a measurement portion 10 which measures, by a predetermined amount, the liquid outflowing through the flow channel 7; a surplus portion 11; a receiving portion 12; and so on. Additionally, on the inspection object acceptor 1, a cover 3 is attached, and, in the cover 3, an inlet 4 for injecting the liquid is formed. In the state where a bottom face of the plate-like member 2 is parallel to the gravity direction (the arrow A direction), and where the inspection object acceptor 1 is revolved around an axis parallel to the gravity direction so as to apply a centrifugal force to the inspection object acceptor 1, an outflow port 9 is provided above the inlet 4 and the liquid sump portion 5 is provided below the outflow port 9 in the gravity direction.

Description

  The present invention relates to a test target receptor, for example, a test target receiver for performing chemical, medical, and biological tests.

  Conventionally, in the field of chemical, medical, and biological examinations, when detecting and quantifying DNA (Deoxyribo Nucleic Acid), enzymes, antigens, antibodies, proteins, viruses, cells and other biological materials, and chemical substances An inspection object receiver called a microchip or an inspection chip used for the above has been proposed (see, for example, Patent Document 1). As shown in FIG. 13, in this test target receptacle 100, the test target liquid or reagent is injected into the internal liquid supply paths 101 and 103, the test target receiver is held horizontally and revolved, Using the centrifugal force generated by the revolution, the liquid is moved to a plurality of mixing tanks in the flow path formed in the test object receptacle, and the inspection is performed. In this apparatus, blood is centrifuged, and then a reaction reagent and its diluted solution are mixed, and a test is performed by a process of mixing sample blood and a reaction reagent.

JP 60-238760 A

  However, when the inspection object receptacle of the invention described in Patent Document 1 is mounted on an inspection apparatus that holds and revolves in parallel with the direction of gravity (hereinafter also referred to as “vertical”), the liquid reservoir shown in FIG. The outlets 102 and 104 are directed in the direction of gravity, and the liquid or reagent to be inspected flows out from the outlets 102 and 104 of the liquid reservoir due to capillary action or gravity. For this reason, there is a problem that the amount of the liquid or reagent to be inspected changes and the inspection cannot be performed accurately.

  The present invention has been made to solve the above-described problem, and realizes an inspection target receptacle in which a liquid to be inspected does not flow out even when the inspection target receptacle is mounted on an inspection device that holds the inspection subject vertically. Objective.

  In order to achieve the above object, in the test object receptacle of the first aspect of the present invention, a liquid reservoir part for storing a liquid to be inspected and a flow path connected to the liquid reservoir part are formed inside, A test object receiver for inspecting the liquid by moving the liquid from the liquid reservoir to the flow path by applying a centrifugal force, wherein the liquid reservoir and the flow path are on the surface. A plate-like member provided in a groove shape, a cover member provided on the surface of the plate-like member, sealing the liquid reservoir and the flow path, provided in the cover member, and provided in the liquid reservoir An inlet for injecting the liquid; and an outlet provided in the liquid reservoir for allowing the liquid to flow out to the flow path. The bottom surface of the plate-like member is parallel to the direction of gravity and parallel to the direction of gravity. In a state where centrifugal force is applied by revolution with a rotation axis, the outlet is in front of the gravity direction. Than inlet provided above, and the liquid reservoir is characterized in that provided below the outlet port.

  In the test subject receptacle of this configuration, with the bottom surface of the plate-like member parallel to the gravity direction, in a state in which centrifugal force is applied by revolution with a rotation axis parallel to the gravity direction, with respect to the gravity direction, Since the outflow port is provided above the injection port and the liquid reservoir is provided below the outflow port, when the liquid to be inspected flows from the injection port, the liquid flows out from the outflow port to the flow path. Can be prevented.

  Moreover, when the said test subject receptacle is made into a horizontal state with the surface facing up, a part of bottom part of the said liquid reservoir part may become a hollow part lower than the lower end part of the said outflow port. When injecting the liquid or reagent to be inspected into the inspection object receiver, place the inspection object in a horizontal state with the surface up, and inject the inspection liquid or reagent. Even if the liquid or reagent to be inspected is vigorously injected, it first accumulates in this recess, so that the liquid in the liquid reservoir can be prevented from flowing out from the outlet.

  Moreover, you may provide the inclined surface formed toward the said outflow port from the edge part by the side of the said outflow port of the bottom face of the said hollow part. In this case, when a centrifugal force is applied to the test subject receptacle, the liquid in the recess of the liquid reservoir can be guided to the inclined surface and smoothly flow into the outlet.

  Moreover, the said hollow part may be formed to the part facing the said injection inlet. It can prevent that the liquid inject | poured from the injection port enters into a hollow part, and a liquid flows out from an outflow port to a flow path.

  In addition, a rib having a predetermined length standing from the bottom of the liquid reservoir is disposed between the side wall of the liquid reservoir on the outlet side and the inlet, and the liquid reservoir has a liquid at the back of the liquid reservoir. It may be erected at an inclination angle that flows. In this case, when the back surface of the test object receptacle is parallel to the direction of gravity and the test object receptacle is in a so-called “vertical state”, the liquid is injected from the injection port by the rib when the liquid is injected from the injection port. Thus, the liquid can be prevented from flowing directly to the outlet.

  The side wall on the outlet side of the liquid reservoir may be inclined in the direction of the centrifugal force toward the outlet. In this case, when a centrifugal force is applied, the liquid can be easily discharged from the outlet by being guided by the side wall.

3 is a plan view of the inspection device 30. FIG. It is a front view of inspection object receptacle 1 of a first embodiment. FIG. 3 is a cross-sectional view in the direction of the arrow of the test object receptacle 1 along the line XX in FIG. 2. It is a longitudinal cross-sectional view of the to-be-inspected receiving body 1 in the X-X line of the state which filled the liquid in the liquid reservoir part 5 horizontally. It is a front view of the test subject receptacle 1 in a state where the liquid reservoirs 5 and 25 are filled with liquid. It is a front view of the state which applied the centrifugal force to the to-be-inspected receiving body 1 in the arrow B direction. It is a front view of the state which rotated 90 degrees of test object receptacles 1 counterclockwise, and added the centrifugal force. It is a front view of the state which returned the inspection object receptacle 1 to the angle of the initial state, and added the centrifugal force. It is a front view of the test object receptacle 31 of 2nd embodiment. FIG. 10 is a cross-sectional view in the direction of the arrow of the inspection object receiver 31 taken along the line XI-XI in FIG. 9. It is a front view of the test object receptacle 51 of 3rd embodiment. FIG. 12 is a cross-sectional view in the direction of the arrow of the inspection object receiver 51 taken along the line XII-XII in FIG. 11. It is a front view of the conventional test object receptacle 100. FIG.

  Hereinafter, a first embodiment of the present invention will be described. In the first embodiment, the inspection apparatus 30 shown in FIG. 1 is mounted so that the bottom surface 17 of the inspection object receiver 1 shown in FIGS. 2 and 3 is parallel to the direction of gravity (the direction of arrow A shown in FIGS. 2 and 3). Revolving and centrifugal force is applied. First, the structure of the inspection apparatus 30 will be briefly described with reference to FIG. As shown in FIG. 1, a rotating disk-shaped turntable 33 is provided on the upper plate 32 of the inspection apparatus 30. A holder angle changing mechanism 34 is provided on the turntable 33. The holder angle changing mechanism 34 is provided with a pair of holders 47 that are inserted and fixed to be inspected and are rotated. A motor (not shown) is provided below the upper plate 32 so as to drive the turntable 33 to rotate. As the turntable 33 rotates about the central portion 35 as an axis, centrifugal force acts in the direction of arrow B on the test object receivers 1 inserted into the holders 47, respectively. Further, the direction of the centrifugal force acting on the test object receptacle 1 can be changed by operating the holder angle changing mechanism 34 to rotate the holder 47 by a predetermined angle.

  Next, the structure of the inspection object receiver 1 will be described with reference to FIGS. FIG. 2 is a front view when the inspection object receiver 1 is mounted on the inspection device 30, and the bottom surface 17 (see FIG. 3) of the inspection object receiver 1 is parallel to the direction of gravity (the direction of arrow A). Yes. As shown in FIGS. 2 and 3, the test object receptacle 1 is composed of a plate-like member 2 having a rectangular shape in front view and a predetermined thickness. As an example of the material of the plate-like member 2, a synthetic resin can be used. A cover member 3 that covers the surface of the inspection target receptacle 1 is attached to the inspection target receptacle 1 on the surface side (right side in FIG. 3). The cover member 3 seals the liquid reservoirs 5, 25, the flow paths 7, 27, the measuring part 10, the surplus part 11 and the receiving part 12 which will be described later. The cover member 3 is composed of a transparent thin plate of a rectangular synthetic resin having the same shape as that of the plate-like member 2 in front view. Further, the cover member 3 is formed with injection ports 4 and 24 for injecting liquid into the liquid reservoirs 5 and 25 in the test object receiver 1.

  As shown in FIGS. 2 and 3, the plate-like member 2 of the inspection object receiver 1 has liquid reservoirs 5 and 25 each formed of a concave portion dug down to a predetermined depth, and liquid is supplied from the liquid reservoirs 5 and 25. Flowing channels 7 and 27, a measuring unit 10 that measures a predetermined amount of the first liquid flowing out from the channel 7, a surplus unit 11 into which the remaining first liquid measured by the measuring unit 10 flows, and weighing The receiving portion 12 into which the first liquid measured by the portion 10 and the second liquid from the liquid reservoir portion 25 flow is formed. Here, an example of the first liquid injected into the liquid reservoir 5 is blood, and an example of the second liquid injected into the liquid reservoir 25 is a reagent.

  The liquid reservoir 5 is a portion for accumulating the first liquid to be inspected injected from the inlet 4 and is dug down to a predetermined depth as shown in FIGS. 3 and 4, and as shown in FIG. Liquid is stored between the member 3 and the bottom 6. In addition, as shown in FIG. 4, when injecting a liquid into the test object receptacle 1, the back surface (bottom surface) of the test object receptacle 1 is set in a horizontal state so as to be orthogonal to the direction of gravity (direction of arrow A). . As shown in FIGS. 2 and 3, an outlet 9 to the flow path 7 for flowing the liquid to the measuring unit 10 is opened in a rectangular shape in front view at the upper part of the liquid reservoir 5. Further, as shown in FIG. 2, the wall portion 8 on the outlet 9 side is inclined in the direction of centrifugal force (in the direction of arrow B shown in FIG. 2) generated by the revolution of the inspection target receptacle 1 by the inspection device 30. Therefore, when a centrifugal force is applied to the test object receiver 1 in the direction of arrow B shown in FIG. 2, the liquid in the liquid reservoir 5 moves up the wall 8 and flows from the outlet 9 to the flow path 7. Inflow. As shown in FIG. 2, in the upper right part of the test object receiver 1, an inlet 24 for introducing the second liquid into the receiving part 12, a liquid reservoir 25, a flow path 27, a wall 28, and The outlet 29 is formed with the same structure as the inlet 4, the liquid reservoir 5, the flow path 7, the wall 8 and the outlet 9.

  Next, the usage method of the test object receptacle 1 of said 1st embodiment is demonstrated. As shown in FIG. 4, the test object receptacle 1 is a first liquid to be inspected from the inlet 4 in a state (horizontal state) in which the bottom surface 17 of the plate-like member 2 is orthogonal to the gravity direction (arrow A direction). (As an example, blood) is injected into the liquid reservoir 5, and a second liquid (as an example, a reagent) is injected into the liquid reservoir 25 from the injection port 24. Next, as shown in FIG. 5, the inspection object receiver 1 is fixed to the holder 47 of the inspection apparatus 30 with the bottom surface 17 (see FIG. 4) of the plate-like member 2 parallel to the gravity direction (arrow A direction). (This state is called "initial state"). Then, the inspection device 30 revolves the inspection target receptacle 1 around the central portion 35 of the turntable 33 as a rotation axis, and applies centrifugal force to the inspection target receptacle 1 in the direction of arrow B as shown in FIG. . Here, the liquid accumulated in the liquid reservoir 5 moves up the wall 8 and flows out from the outlet 9 to the flow path 7, and as shown in FIG. It will be attracted to. Next, the holder 47 rotates 90 degrees counterclockwise, and the test object receiver 1 is in a state where the surplus portion 11 is in the lower direction and the receiving portion 12 is in the upper direction in the direction of gravity as shown in FIG. In this state, since the centrifugal force acts on the test object receiver 1 in the direction of arrow B, the first liquid flows from the flow path 7 into the measuring unit 10 and the remaining liquid flows into the surplus part 11. Therefore, a predetermined amount of the first liquid is measured by the measuring unit 10. Further, the second liquid flows from the flow path 27 into the receiving portion 12.

  Next, the holder 47 rotates 90 degrees clockwise, and centrifugal force is applied in the direction of the arrow to the test object receiver 1 in the state shown in FIG. 7 at the position in the initial state shown in FIG. Here, the first liquid collected and measured in the measuring unit 10 flows into the receiving unit 12 and is mixed with the second liquid. Moreover, the 1st liquid collected in the surplus part 11 is drawn near the lower end part of the surplus part 11 with a centrifugal force, as shown in FIG.

  As described above, in the test object receptacle 1 of the first embodiment, as shown in FIGS. 2 and 3, the bottom surface 17 of the plate-like member 2 is parallel to the direction of gravity (the direction of arrow A) and gravity is applied. The outlet 9 is provided above the inlet 4 with respect to the direction of gravity (in the direction of arrow A) in a state in which centrifugal force is applied by revolution having a rotation axis parallel to the direction, and a liquid reservoir. 5 is provided below the outlet 9. With this structure, it is possible to prevent the liquid from flowing out from the outlet 9 to the flow path 7 when the liquid to be inspected flows from the inlet 4.

  Next, with reference to FIG.9 and FIG.10, the test object receptacle 31 of 2nd embodiment is demonstrated. Below, a different point from the test object receiver 1 of 1st embodiment is demonstrated. The inspection object receiver 31 of the second embodiment is different from the inspection object receiver 1 of the first embodiment in that the depressions 14 and 54 are formed in the liquid reservoirs 5 and 25 and the depression 14. The inclined surface 13 is formed from the end on the outflow port 9 side toward the outflow port 9. In addition, the recessed part 54 is also provided with a slope similar to the inclined surface 13.

  The dents 14 and 54 are circular dents when viewed from the front. When the dent portions 14 and 54 are in a horizontal state with the surface of the test object receiver 31 facing upward (when FIG. 12 is rotated 90 degrees counterclockwise), the outlet 9 It is a hollow part lower than the lower end part 18. With this recess 14, the test object receiver 31 is placed in a horizontal state with the surface facing up, and when the first liquid is injected into the liquid reservoir 5 from the inlet 4, the first is first applied to the recess 14. Since it accumulates, it can prevent that the 1st liquid flows out from the outflow port 9. FIG.

  In addition, as shown in FIG. 10, in the inspection target receptacle 31, an inclined surface 13 having a predetermined inclination is formed from the end portion of the bottom portion 6 of the recess portion 14 on the outflow port 9 side toward the outflow port 9. As shown in FIG. 9, when a centrifugal force is applied to the test object receiver 31 in the direction of arrow B, the liquid in the recess 14 of the liquid reservoir 5 is guided to the inclined surface 13 and smoothly flows to the outlet 9. Can flow in.

  Next, with reference to FIG.11 and FIG.12, the test object receptacle 51 of 3rd embodiment is demonstrated. Below, a different point from the test object receiver 31 of 2nd embodiment is demonstrated. The inspection object receiver 51 of the third embodiment is different from the inspection object receiver 31 of the second embodiment in that the liquid reservoir 5 is provided with ribs 15 and the liquid reservoir 25 is provided with ribs 55. Is a point. The rib 15 is provided with a predetermined length from the bottom 6 between the inlet 4 and the wall 8 on the outlet 9 side. The rib 55 is also erected from the bottom 6 by a predetermined length between the inlet 24 and the wall 28 on the outlet 29 side. FIG. 12 shows a cross section 16 of the rib 15. As shown in FIG. 12, the rib 15 has a height that comes into contact with the cover member 3 from the bottom 6 of the liquid reservoir 5. Furthermore, as shown in FIG. 12, the lower end portion of the rib 15 (the end portion in the gravity direction of arrow A) faces the inner portion of the liquid reservoir portion 5 in the gravity direction (the arrow A direction), and the upper end portion flows. A predetermined length is extended in the direction of the outlet 9. In addition, the inclination angle of the rib 15 is an angle inclined in the direction of the wall portion 8 shown in FIG.

  In the inspection object receiver 51 of the third embodiment, the rib 15 is provided, so that the back surface of the inspection object receiver 51 is parallel to the direction of gravity and the inspection object receiver 51 is in a so-called “vertical state”. When the liquid is injected from the inlet 4, the liquid is guided by the rib 15 to the back of the liquid reservoir 5 in the direction of gravity (arrow A direction) and is difficult to flow to the outlet 9 side. The outflow from the outlet 9 can be prevented. Moreover, since the upper end part of the rib 15 is extended toward the outflow port 9, when centrifugal force is given to the arrow B direction shown in FIG. By being guided, the liquid in the liquid reservoir 5 can completely flow out from the outlet 9 to the flow path 7.

  The present invention is not limited to the above embodiment, and various modifications can be made. For example, the material of the test object receivers 1, 31, 51 is not particularly limited, and polyethylene terephthalate (PET), polybutylene terephthalate (PBT), polymethyl methacrylate (PMMA), polycarbonate (PC), polystyrene (PS), polypropylene (PP), polyethylene (PE), polyethylene naphthalate (PEN), polyarylate resin (PAR), acrylonitrile-butadiene-styrene resin (ABS), vinyl chloride resin (PVC), polymethylpentene resin (PMP), polybutadiene resin Organic materials such as (PBD), biodegradable polymer (BP), cycloolefin polymer (COP), and polydimethylsiloxane (PDMS) can be used. In addition, an inorganic material such as silicon, glass, or quartz may be used. Further, in the inspection object receivers 1, 31, 51, two liquid injection paths are provided, but one, three, four, etc. may be provided as appropriate.

DESCRIPTION OF SYMBOLS 1 Inspection object receptacle 2 Plate-shaped member 3 Cover member 4 Inlet 5 Liquid reservoir part 6 Bottom part 7 Flow path 8 Wall part 9 Outlet 10 Metering part 11 Surplus part 12 Receiving part 13 Inclined surface 14 Recessed part 15 Rib 16 Rib cross section 17 bottom 18 lower end 30 inspection device 31 inspection object receiver 32 upper plate 33 turntable 34 holder angle changing mechanism 35 central portion 47 holder 51 inspection object receiver

Claims (6)

A liquid reservoir for storing a liquid to be inspected and a flow path connected to the liquid reservoir are formed inside, and the liquid is moved from the liquid reservoir to the flow path by applying a centrifugal force. , An inspection target receiver for inspecting the liquid,
A plate-like member in which the liquid reservoir and the flow path are provided in a groove shape on the surface;
A cover member that is provided on the surface of the plate-like member and seals the liquid reservoir and the flow path;
An inlet provided in the cover member for injecting the liquid into the liquid reservoir;
An outflow port provided in the liquid reservoir and allowing the liquid to flow into the flow path;
With
With the bottom surface of the plate-like member parallel to the gravitational direction and centrifugal force is applied by revolution having a rotation axis parallel to the gravitational direction, the outlet port is more than the inlet port with respect to the gravitational direction. And the liquid reservoir is provided below the outflow port.   The bottom of the liquid reservoir is a recess that is lower than the lower end of the outlet when the test receiver is in a horizontal state with the surface facing up. 1. The test object receiver according to 1.   The test object receptacle according to claim 2, further comprising an inclined surface formed toward an outflow port from an end of the bottom surface of the hollow portion on the outflow port side.   The test object receptacle according to claim 2 or 3, wherein the hollow portion is formed up to a portion facing the injection port.   A rib of a predetermined length standing from the bottom of the liquid reservoir flows between the side wall on the outlet side of the liquid reservoir and the inlet, and allows liquid to flow into the back of the liquid reservoir. The test object receptacle according to any one of claims 1 to 4, wherein the test object receptacle is set up at an inclination angle.   6. The test object receptacle according to claim 1, wherein a side wall of the liquid reservoir on the outlet side is inclined in the direction of the centrifugal force toward the outlet.

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